Inhibitory Effects of Tripterine on Wear Particle-Induced Cellular Inflammatory Reaction / 天津医药

Objective To investigate the effect of tripterine on wear particle-induced inflammatory reaction by cell culture in vitro. Methods Wear particles from artificial joints were prepared by vacuum ball milling, and made into the par-ticle suspension by using serum-free medium. RAW264.7 cells were cultured, subcultured and divided randomly into four groups according to different treatment factors:blank control group (group A), wear particle group (group B), wear particle+tripterine group (group C) and tripterine group (group D). After 24 hours, the toxicity of tripterine was detected by CCK-8 as-say. ELISA was used to detect the expressions of tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β). RT-PCR was used to detect the expressions of TNF-α, IL-1βand nuclear factor-κB (NF-κB) at the gene level. Western blot assay was used to detect the expression of NF-κB at the protein level. Results The results showed that 1 mg/L tripterine was little cytotoxic. The expressions of pro-inflammatory cytokines and the activation of NF-κB were significantly higher in wear particle group than those in blank control group (P＜0.05), which were significantly decreased after the treatment with tripter-ine (group C, P＜0.05). Conclusion Tripterine can inhibit the wear particle-induced expression and release of pro-inflam-matory cytokines at the gene level and protein level and the activation of NF-κB in RAW264.7 cells.

In vitro studies of multiwalled carbon nanotube/ultrahigh molecular weight polyethylene nanocomposites with osteoblast-like MG63 cells

Carbon nanotubes are highly versatile materials; new applications using them are continuously being developed. Special attention is being dedicated to the possible use of multiwalled carbon nanotubes in biomaterials contacting with bone. However, carbon nanotubes are also controversial in regards to effects exerted on living organisms. Carbon nanotubes can be used to improve the tribological properties of polymer/composite materials. Ultrahigh molecular weight polyethylene (UHMWPE) is a polymer widely used in orthopedic applications that imply wear and particle generation. We describe here the response of human osteoblast-like MG63 cells after 6 days of culture in contact with artificially generated particles from both UHMWPE polymer and multiwalled carbon nanotubes (MWCNT)/UHMWPE nanocomposites. This novel composite has superior wear behavior, having thus the potential to reduce the number of revision hip arthroplasty surgeries required by wear failure of acetabular cups and diminish particle-induced osteolysis. The results of an in vitro study of viability and proliferation and interleukin-6 (IL-6) production suggest good cytocompatibility, similar to that of conventional UHMWPE (WST-1 assay results are reported as percentage of control ± SD: UHMWPE = 96.19 ± 7.92, MWCNT/UHMWPE = 97.92 ± 8.29 percent; total protein: control = 139.73 ± 10.78, UHMWPE = 137.07 ± 6.17, MWCNT/UHMWPE = 163.29 ± 11.81 µg/mL; IL-6: control = 90.93 ± 10.30, UHMWPE = 92.52 ± 11.02, MWCNT/UHMWPE = 108.99 ± 9.90 pg/mL). Standard cell culture conditions were considered as control. These results, especially the absence of significant elevation in the osteolysis inductor IL-6 values, reinforce the potential of this superior wear-resistant composite for future orthopedic applications, when compared to traditional UHMWPE.

Preparation of metallic wear particles around joint prosthesis by vacuum ball mill in vitro / 中国矫形外科杂志

[Objective]To investigate a method of in vitro preparation and separation for metallic wear particles around joint prosthesis,and evaluate its feasibility in medical experiments of prosthetic loosening.[Method]Metallic wear particles were made around joint prosthesis by vacuum ball mill in vitro.Particle size,size distribution,chemical composition and shape were characterized by element trace analysis,laser countersizer,scanning electron microscopy.Metal particles were co-cultured with RAW264.7 cells,and its cytotoxicity was detected.[Result]Metal particles made by vacuum ball mill in vitro was similar to particles extracted in vivo(

Experimental study on a method of in vitro preparation and seperation for metallic prosthesis wear particles / 中国矫形外科杂志

[Objective]To design a method of in vitro preparation and seperation for metallic wear particles around joint prosthesis and evaluate its feasibility in medical experiments.[Method]Ti-6Al-4V and Co-Gr-Mo alloys were used to make two friction jars respectirely. National inventive patent applied number 03142073.7.Lots of quadrate blocks made of the same materials are put into the jars respectively,which were then.lubri cated by man-made body fluid and vibrated on a bottle shaker.After 21 days the fluid was harvested and centrifuged to get the produced wear particles.The collected particles were studied by using element trace analysis,laser countersizer and scanning electron microscopy.The J774.A1 macrophages cultured together with these particles for 24 hours were observed under inverted phase contrast microscopy and transmission electron microscopy.[Result]A got great amounts of metallic particles with 1?m diameter coned beproduce using this method.The aver age diameter of titanium alloys(Dv90) is 1.011 and that of Co-Gr-Mo is 1.010.Particle size distribution had good consistency in different materials.Under scanning electron microscopy ,the particles had irregular shapes just like those got from revision operations.The particles taken into the J774.A1 macrophages could be seen under inverted p hase contrast microscopy and transmission electron microscopy.[Conclusion]This method is good enough to producl lots of metallic wear particles mosth like those around total joint prosthesis and can be used in further in vivo and in vitro studies about joint prosthesis loosening.

Preventive and Mitigative Effects of Alendronate on Osteolysis induced by Particulate Debris: Quantitative and Comparative Analysis Using Mouse Calvarial Model / 대한정형외과학회잡지

PURPOSE: Wear particle induced osteolysis is a serious complication in total joint arthroplasty. The purpose of this study was to compare the preventive and mitigative effects of alendronate on particle induced osteolysis among particles using mouse calvarial model. MATERIALS AND METHODS: Seventy-two mice were randomized into sham group, Ti6Al4V group, CoCr- Mo group and Ultra High Molecular Weight Polyethylene (UHMWPE) group according to the particles treated to the calvarial tissues. Each group was divided into control subgroup which received no alendronate, preventive subgroup which received alendronate for 4 weeks after particle distribution and therapeutic subgroup which received alendronate for 3 weeks after 1 week delay from particle distribution. We evaluated the effects quantitatively using histomorphometry. Number of osteoclast, fibrous thickness, eroded bone surface area, bone thickness and bone volume were measured and compared. RESULTS: Alendronate showed significant preventive and mitigative effects on osteolysis induced by Ti6Al4V particles and showed significant preventive and somewhat less mitigative effect on osteolysis by CoCrMo particles. On UHMWPE particles, alendronate showed no preventive and mitigative effects. CONCLUSION: Alendronate may be useful in preventing osteolysis induced by metal particles but had no effect on ostelysis induced by UHMWPE particles.

An Inguinal Mass associated with Polyethylene Wear Debris after a Total Hip Arthroplasty: A Case Report / 대한정형외과학회잡지

Ostelolysis and implant loosening by wear particles after a total hip arthroplasty is a well-documented phenomenon. Howerver, the occurrence of a soft tissue mass around the hip joint after a total hip arthroplasty is rare. We experienced a case of an inguinal mass associated with polyethylene wear particles. An excision of the mass and revision arthroplasty for a polyethylene liner and metal head were performed successfully via a separate incision. We describe this rare case with a review of the relevant literature.