ABSTRACT
Methods: Twenty Sprague Dawley rats were randomly divided into 2 groups ( n=10). DermalGen were implanted subcutaneously into the dorsum of rats in experimental group, and the rats in control group were treated with sham-operation. At 3, 7, and 15 days and 1, 3, 6, and 12 months after operation, the samples of experimental group were harvested and gross observation, histological observation, CD31 immunohistochemical staining, and transmission electron microscope observation were taken to observe the inflammatory reaction, angiogenesis, and collagen arrangement. The skin tissues of the control group at 12 months were observed and compared.
ABSTRACT
Objectives To establish an ideal cellular model for the study of infection of porcine endogenous retrovirus in intro and in vivo and to lay the foundation for evaluating the biological safety of xenotransplantation. Methods Porcine skin fibroblasts were cultured by tissue pieces, cold trypsin, and heat trypsin, respectively. The merit and defect of these methods were analyzed. Results Porcine skin fibroblast line was established and the tissue piece method was superior to trypsin methods. Conclusion Culture of porcine skin fibroblasts with tissue pieces is superior to trypsin method, with the characteristics of low cost and easy operation. The established porcine skin fibroblast cell line supplies an ideal cell model for the study of infection of porcine endogenous retrovirus in vitro and in vivo, provides a new method for the evaluation of biological safety of xenotransplantation, and plays an important role in the skin culture model of production of cloned animals.