ABSTRACT
Objective:To explore the effects and mechanism of zedoary turmeric oil and its active components on the vascular endothelial growth factor A （VEGFA）， signal transducer and activator of transcription 3 （STAT3）， and mechanistic target of rapamycin （mTOR） in the ovarian cancer （OC）. Method:Network pharmacology technology was employed to analyze the mechanism of Curcumae Rhizoma on OC. Bioinformatics was used to analyze the expression of VEGFA， STAT3， and mTOR in OC and the effect on the prognosis of OC to explore the feasibility of zedoary turmeric oil in regulating VEGFA， STAT3， and mTOR in OC.The xenograft tumor model of nude mice was established， and the effects of zedoary turmeric oil and its active components on VEGFA， STAT3， and mTOR in OC were observed by hematoxylin-eosin （HE） staining， real-time fluorescence-based quantitative polymerase chain reaction （Real-time PCR）， Western blot， and immunohistochemistry （IHC）. Result:Bioinformatics analysis and literature research showed that VEGFA， STAT3， and mTOR played a special regulatory role in the occurrence and development of OC， and were potential key targets for the proliferation of OC. Network pharmacology analysis revealed that Curcumae Rhizoma could regulate multiple disease targets of OC， and mediate VEGFA， STAT3， and mTOR in OC through these multiple targets. As demonstrated by HE staining， the tumor cells in the model group were densely arranged， with no erosion on the edge and no vesicles inside. Compared with the model group， the cell density in other treatment groups was reduced， and strip-shaped erosion on the edge and small empty vesicles were observed in the tumor tissue， especially in the zedoary turmeric oil group. According to the results of Real-time PCR and IHC， zedoary turmeric oil and its active components could inhibit the mRNA and protein expression of VEGFA， STAT3， and mTOR in the OC tissue （<italic>P</italic><0.05）. Conclusion:Zedoary turmeric oil and its active components could reduce the expression of VEGFA， STAT3， and mTOR in tumor tissue of nude mice， and inhibited the proliferation of OC through VEGFA， STAT3， and mTOR.
ABSTRACT
Because of no specific drug for the treatment of coronavirus disease 2019 (COVID-19), a number of treatment plans for COVID-19 had been released by national medical treatment authorities. The efficacy of zedoary turmeric oil and its preparation for antiviral and pulmonary fibrosis treatment had been confirmed by many basic studies and clinical applications. It was speculated that Zedoary Turmeric Oil Injection could be used in the clinical diagnose and treatment of COVID-19, especially in the treatment of pulmonary fibrosis caused by pulmonary interstitial changes, antidiarrheal and fever. In addition, the compatibility of Zedoary Turmeric Oil Injection with other antiviral and antibiotic drugs suggested that it could be used to reduce drug-induced liver injury in the treatment of COVID-19 patients, and improve the therapeutic effect, which provided a theoretical basis for the scientific use of zedoary turmeric oil and its injection in the treatment of COVID-19.
ABSTRACT
OBJECTIVE: To prepare zedoary turmeric oil compound liposomes (ZTOC-LPS) and evaluate its quality.METHODS: The preparation method of liposome, the addition amount of soybean phosphatidylcholine (SPC), ratio of SPC to cholesterol (CH) in lipid, drug-lipid ratio of zedoary turmeric oil (ZTO), drug-lipid ratio of tretinoin in formulation, and water bath temperature were screened using encapsulation efficiency and drug-loading amount of ZTO (represented by germacrone) and tretinoin as investigation indexes. Quality evaluation and primary stability investigation were conducted for liposomes prepared by optimal preparation technology. RESULTS: The optimal preparation method was ethanol injection method; The optimal preparation technology were SPC 4 mg in 1 mL lipid, the mass ratio of SPC to CH 3:1, the ratio of ZTO to lipid 1:9, the ratio of tretinoin to lipid 1:70, water bath temperature of 55 ℃. Encapsulation efficiencies of ZTO and tretinoin were (64. 63 ± 1. 00)％ and (90. 33 土 0. 72)％ in 3 batches of ZTOC-LPS, respectively. Drug-loading amount of ZTO and tretinoin were (9. 09 ± 0. 14)％ and (1. 43 ± 0. 02)％, respectively. Particle size was (257. 41 ± 7. 58) nm, Zeta potential was (-38. 77 ± 0. 81) mV,PDI was 0. 10 ± 0. 04; the results of centrifugal acceleration test showed that the liposomes had good physical stability. No obvious change was found in each investigation index of ZTOC-LPS that stored at (4 ± 2) ℃ for 1 month. CONCLUSIONS: Established preparation technology is simple and feasible, the quality of the prepared ZTOC-LPS conforms to the requirements, and it can provide reference for the following research of ZTOC-LPS.
ABSTRACT
Objective To explore the effects of zedoary turmeric oil on proliferation and apoptosis of SW1463 cell line and the expression of Caspase-3,Bax and Bcl-2.Methods Volatile oil from Curcumae Rhizoma in Guizhou was extract by steam distillation,which was used to intervene SW1463 cells for 24,48 and 72 h at concentration of 40,80,120,160,200,240 and 280 mg/mL.MTT method was used to detect the inhibitory rate of zedoary turmeric oil on SW1463 cell proliferation.Effects of different concentrations of zedoary oil on apoptosis of SW1463 cells were observed by Giemsa staining.Western blotting was used to detect Capase-3,Bax and Bcl-2 protein expression.Results Zedoary turmeric oil inhibited the proliferation of SW1463 cells and showed a time dose correlation,and half maximal inhibitory concentration (IC50) of 24,48 and 72 h was 144.33,134.11 and 120.04 mg/L,respectively.Giemsa staining showed obvious morphological characteristics of apoptotic cells.Western blotting results showed that compared with control group,the expression of Caspase-3 and Bax in cells treated with zedoary turmeric oil for 24 h were significantly up-regulated (P ＜ 0.05),and the expression of Bcl-2 protein was significantly down-regulated (P ＜ 0.05).Conclusion Zedoary turmeric oil can obviously inhibit the proliferation of SW1463 cells and induce apoptosis,which may be related to the up-regulation of Caspase-3 and Bax protein expression and down-regulation of Bcl-2 protein expression.
ABSTRACT
Objective To explore the effects of zedoary turmeric oil on proliferation and apoptosis of SW1463 cell line and the expression of Caspase-3,Bax and Bcl-2.Methods Volatile oil from Curcumae Rhizoma in Guizhou was extract by steam distillation,which was used to intervene SW1463 cells for 24,48 and 72 h at concentration of 40,80,120,160,200,240 and 280 mg/mL.MTT method was used to detect the inhibitory rate of zedoary turmeric oil on SW1463 cell proliferation.Effects of different concentrations of zedoary oil on apoptosis of SW1463 cells were observed by Giemsa staining.Western blotting was used to detect Capase-3,Bax and Bcl-2 protein expression.Results Zedoary turmeric oil inhibited the proliferation of SW1463 cells and showed a time dose correlation,and half maximal inhibitory concentration (IC50) of 24,48 and 72 h was 144.33,134.11 and 120.04 mg/L,respectively.Giemsa staining showed obvious morphological characteristics of apoptotic cells.Western blotting results showed that compared with control group,the expression of Caspase-3 and Bax in cells treated with zedoary turmeric oil for 24 h were significantly up-regulated (P ＜ 0.05),and the expression of Bcl-2 protein was significantly down-regulated (P ＜ 0.05).Conclusion Zedoary turmeric oil can obviously inhibit the proliferation of SW1463 cells and induce apoptosis,which may be related to the up-regulation of Caspase-3 and Bax protein expression and down-regulation of Bcl-2 protein expression.
ABSTRACT
OBJECTIVE:To study the distribution characteristics of germacrone from zedoary turmeric oil (ZTO) in each tis-sue of mice,and to provide reference for further application of zedoary turmeric oil. METHODS:30 KM mice were given zedoary turmeric oil 0.5 mL;6 mice were randomly selected 1,2,4,8,12 h after medication,respectively. The contents of germacrone in heart,liver,spleen,lung and kidney tissues were determined by HPLC. 15 KM mice were selected,medication and sampling method were same as above;3 mice were collected at each time point respectively. The fluorescence intensity of germacrone in above sections were observed by fluorescence. The same number of mice were selected as control in 2 trials. RESULTS:The con-centration of germacrone in each tissue 1-4 h increased gradually as time and reached the peak value at 4 h. The contents of ger-macrone in liver and spleen were significantly higher than in heart and lung. The concentrations of germacrone in each tissue were ranked as liver>spleen>kidney>heart>lung. The results of fluorescence intensity observation was same as above results. CON-CLUSIONS:Results of 2 methods show same distribution characteristics of germacrone in mice tissues,and indicate that ger-macrone is distributed more in liver,spleen and kidney tissues and less in heart and lung.
ABSTRACT
Objective: To establish the optimal prescription and technique for preparing zedoary turmeric oil (ZTO) microcapsule. Methods: Using the diameter of microcapsule, microcapsule form, and embedding rate as indexes, the ratio of capsule materials, ratio of core material and capsule material, content of dry matter, content of additive, speed of emulsifying, time of emulsifying, temperature of wind and the power of feed were studied. Results: The optimal conditions for preparation of spray drying of ZTO microcapsule were as follows: gum Arabic-gelatin (1.0∶1.0), core material-capsule material (0.30∶1.0), PEG6000 content of 2%, dry matter content of 20%; The optimal technique was as follows: The emulsion speed was 10 600 r/min, emulsifying time was 9 min, temperature of inlet air was 160 ℃, and feed power was 6%. According to the optimum experimental conditions, the microcapsule was round relatively, the surface density was well, the particle size of microcapsule appeared uniform distribution, most concentrates in 1.0—2.5 μm, and showed good normal distribution, the average particle size was 1.913 μm, the embedding rate could reach 75.4%. Conclusion: This experiment can increase the stability of ZTO, cover up its bad smell, and raise the utilization ratio of drugs, the repeatability is also good.
ABSTRACT
OBJECTIVE:To investigate toxic reaction of Compound zedoary turmeric oil cream in experimental rats with long-term consecutive transdermal administration,and to provide reference for safe use of it in the clinic. METHODS:60 SD rats were randomly divided into blank control (cream matrix) group,Compound zedoary turmeric oil cream intact skin and damaged skin low-dose and high-dose(5%,10%)groups,with 12 rats in each group,half male and half female. All of them were given relevant medicine twice a day. 92 d consecutive medication later,general situation of rats were observed,and body weight,blood routine(WBC,RBC,HGB,LYMPH,etc.)and blood biochemical indicators(AST,ALT,PA,etc.)were all detected;systemati-cal observation of organs,organ coefficient calculation and histopathology examination were carried out. RESULTS:There was no statistical significance in those indicators between Compound zedoary turmeric oil cream groups and blank control group (P>0.05),except hemoglobin decreased in intact skin low-dose group,while hemoglobin decreased,LYMPH and PA increased in dam-aged skin high-dose group(P<0.05). Pathology results showed that Compound zedoary turmeric oil cream had no significant toxici-ty for the main organs. CONCLUSIONS:Compound zedoary turmeric oil cream has no long-term toxicity to experimental rats.
ABSTRACT
AIM:To develop a new method of gas chromatography for simultaneously determining curcumol contentin and β-elemene in Zedoary Turmeric Oil.METHODS:Separation and quantification were achieved on a quartz capillary column(30 m×0.32 mm)of PEG with a column temperature gradient from 80℃(holding for 7 min)to 140℃ at a rate of 20℃/min,holding for 15 min,taking N2 as loader.The temperature for gasification was 210℃;the temperature for detection was 200℃;the compressive stress of column was 0.27 MPa.RESULTS:Under the optimal chromatographic conditions,the linear range of curcumol contentin was within 0.230 5-3.23 mg/mL and the linear range of the β-elemene was within 0.237 5-3.33 mg/mL.The average recovery of curcumol contentin was 100.4% with a relative standard deviation(RSD)of 1.1% and β-elemene was 100.2% with a RSD of 2.2%.CONCLUSION:The results show that it is a simple,rapid,accurate and sensitive method which is suitable for analysis of complex component(curcumol contentin and β-elemene)in Zedoary Turmeric Oil at the same time.
ABSTRACT
AIM: To establish the method of fingerprinting analysis onZedoary turmeric oil by GC. Zedoary turmeric oil for the quality control of reference. METHODS: GC method was used to determine the fingerprint and its similarity of Zedoary turmeric oil calculated on Excel and cluster analysis. RESULTS: Seventeen samples of Zedoary turmeric oil indicated that there was a little similarity among them. Based on the cluster analysis, all the Zedoary turmeric oils could be divided into two categories, self-made and market purchasing oil, the latter oils purchased from Liaoning province and Jiangsu province varied considerably. Every category had a common fingerprint mode with clear resemblance. CONCLUSION: When finding the difference between the self-made and the marketpurchasing Zedoary turmeric oil, quality control requires serious consideration.
ABSTRACT
Objective To observe therapeutic effects of compounded zedoary turmeric oil suppository com-bined with microwave therapy on cervical erosion. Methods Women with cervical erosion were randomly divided in-to two groups: single microwave therapy group (control group) and compounded zedoary turmeric oil suppository com-bined with microwave therapy group (observation group). The chnical cure rate, obvious effective rate and ineffective rate were compared between two groups. Results In mild erosion, the clinical cure rate, obvious effective rate and ineffective rate were 87.8%, 12.2% ,0 respectively in the observation group. In tbe control group were 85.7%, 14.3%,0 respectively, and there was no significant difference between them (P >0.05); In moderate erosion, were 86.1%, 12.8%, 1.2% respectively in the observation group; were 60.9%, 31.9%, 7.2% respectively in the control group and there was significant difference between them (P < 0.01); In severe erosion, were 59.4%, 34.4%, 6.2% respectively in the observation group; were 29.17%, 41.7%, 29.2% respectively in the control group, and there was significant difference between them (P < 0.05). Conclusion Single microwave therapy to the mild erosion was suf-ficient; nevertheless, compounded zedoary turmeric oil suppository combined with microwave therapy to moderate and severe erosion was better than single microwave therapy.
ABSTRACT
Objective To observe the therapetic effects of rotavirus enterocolitis by zedoary turmeric oil and glucose injection. Methods 120 cases were divided into therapatic group and control group at random,with 60 cases in each group. In the control group,ribavirin was used,while in the therapetic group,zedoary turmeric oil and glucose injection was added,without antibiotics in both groups. If dehydration were found,ORS or rehydration by vein would be used to rectify the disorder of water and electrolyte. Results In the therapetic group, it took much shorter time to stop diarrhea than the contrast group( P < 0.05 ). The difference between total disease processes of these two groups is extremely significant(P< 0.01 ). Conclusion Using zedoary turmeric oil and glucose injection with ribavirin to treat rotavirus enterocolitis has good therapetic effect.
ABSTRACT
OBJECTIVE:To observe the stabilities of zedoary turmeric oil and glucose injection mixing with 5 kinds of antibiotics METHODS:The contents of curcamol in mixed fluid were detected with UV spectrophotometry,and the external appearance and value of pH were observed RESULTS:After mixing zedoare turmeric oil and glucose injection with 5 kinds of antibiotics,no gas and sediment were found Values of pH were different in the 5 simples,and did not change markedly over 3 hours The concentration of curcumol was markedly reduced and the sample turned to brown in mixing with cefoperazone sodium;the concentration was declined in mixing with ceftriaxone sodium and decreased 6% with cefradine and was not markedly reduced in mixing with clindamycin and netilmicin CONCLUSION:This preparation is compatible with netilmicin and clindamycin,however,it is incompatible with cefoperazone and ceftriaxone
ABSTRACT
AIM: To study a method for the determination of Zedoary turmeric oil microsphere (ZTO-MS). METHODS: ZTO-MS was extracted supersonically by solvent and then colored by sulfuric acid-vanillin reagent. The absorbance was measured by visible-spectrophotometry at 508nm. At the same time, ZTO-MS also could be determined by HPLC. RESULTS: The content of Zedoary turmeric oil was above 60% and the contents of Curdione、Curcuma、Germacrone were 6.88%,0.95%,5.0%, respectively, in ZTO-MS. CONCLUSION: Two methods for the determination of ZTO-MS are accurate, reliable; the method of visible-spectrophotometry is convenient and appropriate for determination for content of oil in ZTO-MS, while the method of HPLC gains the advantage for qualitative and quantitative analysis of components in ZTO-MS.
ABSTRACT
AIM: To establish the method of fingerprinting analysis on Zedoary turmeric oil by GC.Zedoary turmeric oil for the quality control of reference. METHODS: GC method was used to determine the fingerprint and its similarity of Zedoary turmeric oil calculated on Excel and cluster analysis. RESULTS: Seventeen samples of Zedoary turmeric oil indicated that there was a little similarity among them.Based on the cluster analysis,all the Zedoary turmeric oils could be divided into two categories,self-made and market purchasing oil,the latter oils purchased from Liaoning province and Jiangsu province varied considerably.Every category had a common fingerprint mode with clear resemblance. CONCLUSION: When finding the difference between the self-made and the market purchasing Zedoary turmeric oil,quality control requires serious consideration.
ABSTRACT
90% in 20 min and more higher than the control tablets or capsule. CONCLUSION: The optimum formulation suits to slightly soluble drugs with different o/w distribution coefficient.It can provide reference for application of the SMEDDS the practical cases.
ABSTRACT
AIM: To establish a HPLC method for determination of curcumol and germacrone in Zedoary Turmeric Oil and Glucose Injections. METHODS: RP-HPLC was applied for quantitative analysis of curcumol and germacrone. Hypersil ODS2 column (250 mm? 4.6 mm, 5 ?m) and gradient elution was used. Mobile phase A was acetonitrile and mobile phase B was water. Phase A time-percentage composition was as follows:0-3 min,45%;3-30 min,45%→65%;30-38 min, 65%; 38-45 min, 65%→90%;45-55 min,90%→100%;55-65 min,100%. The flow rate was at 1.0 mL?min -1 . Diode array detector was set at 214 nm and column temperature was at 25 ?C. RESULTS: The calibration curves of curcumol and germacrone were linear in the range of 0.26 - 6.5 ?g(r= 0.999 9 ) and 0.112 5 - 2.812 5 ?g(r=1), The average recoveries of them were 102.1% and 98.8% (n=5), respectively. CONCLUSION: Curcumol and germacrone can be determined by the method, and this will improve the quality control of Zedoary Turmeric Oil and Glucose Injections.
ABSTRACT
Aim Curcumol and Zedoary Turmeric Oil (ZTO) were prepared to liposome, which were used to study the anti-hepatoma effect, and its mechanism that was expected to provide theoretical support for the clinic anticarcinomal use of Zedoray Rhizome Abstracts.Methods Curcumol and ZTO were prepared to liposome by mechanical diffusion and ultrasonic techniques.Proliferation of HepG2 cell in vitro was observed with curcumol and ZTO by MTT assay. Apoptosis of cells was observed by staining with Hoechst 33258/PI and laser confocal scanning microscopy (LCSM). Expression of COX-2 mRNA and VEGF mRNA of HepG2 cell was investigated by RT-PCR.Results Envelopment ratios of curcumol liposome and ZTO liposome were 86.2% and 74.5%,respectively.Curcumol and ZTO inhibited proliferation of HepG2 cell by MTT assay.Compared with control group, Curcumol and ZTO increased the apoptosis rate of HepG2 cell significantly (P