Effects of G-CSF Gene Therapy bn the Number and Functions of Peripheral Neutrophils of Colon Adenocarcinoma-Bearing Mice Receiving High-Dose Chemotherapy / 中国肿瘤生物治疗杂志

Neutrophils play important role in anti - tumor response of tumor-bearing host as a kind of important effector cells. In order to identify the anti-tumor mechanisms of G-CSF gene therapy, we investigated the number and functions of the peripheral neutrophils in the C-26 colon adenocarcinoma-bearing mice receiving G - CSF gene therapy and high - dose chemotherapy. After G - CSF gene therapy, the number of neutrophils in peripheral blood was increased markedly in C-26 mice receiving high - dose 5 - Fu as compared with control groups including in vivo administration of rhG - CSF. The more potent cytotoxicity to C - 26 cells could be detected. The phagocytic activity, the secretion of IL-1, TNF, NO of the neutrophils were significantly enhanced. These data showed that G - CSF gene therapy can increase the number of neutrophils, activate the functions more effectively than in vivo administration of rhG - CSF.

Antitumor Effects of G-CSF Gene Therapy on Colon Adenocarcinoma-Bearing Mice Receiving High-Dose Chemotherapy / 中国肿瘤生物治疗杂志

Granulocyte colony - stimulating factor (G - CSF) is a hematopoietic growth factor that is responsible for the differentiation and proliferation of hematopoietic progenitor cells to mature granulocyte, and can increase the number of peripheral neutrophils. It has been demonstrated that it could inhibit the metastasis of the murine tumors in spontaneous and experimental metastasis models by in vivo administration of recombinant human G - CSF. In order to examine the antitumor effect of G - CSF gene therapy on mice receiving high - dose chemotherapy, C - 26 colon adenocarcinoma - bearing mice which were prepared by S. c. injection of 1?105C-26 cells were i. p. injected with rhG-CSF (2?g/day?14day) or implanted with 1?107 collagen encapsulated NIH3T3-G-CSF cells which secrete high level of G - CSF after gene transfection. In our experiment, rhG-CSF could inhibit the tumor growth and extend the survival time of early stage C-26 bearing mice. However, G - CSF gene therapy could inhibit the tumor growth and prolong the survival both in early or middle stage C-26 mice. The results showed that both rhG - CSF and G - CSF gene therapy have exact antitumor effect and G - CSF gene therapy show more effective than rhG - CSF in vivo. Then we investigated the therapeutic effects of G - CSF gene therapy on C-26-bearing mice receiving high - dose chemotherapy (5-Fu 150mg/mice i. p.) . More effective results could be observed in C-26 - bearing mice receiving high dose chemotherapy after G - CSF gene therapy. The results also suggested that G-CSF gene therapy can inhibit the tumor growth more effectively both in C-26-bearing mice or C-26-bearing mice receiving high - dose chemotherapy.

Treatment of Erythroplasia of Queyrat with Topical 5 % 5 - Fluorouracil Cream under Occlusion / 대한피부과학회지

We herein reported a typical case of erythroplasia of Queyrat in a 35-year-old male patient who had a 6 month-duration of a erythroplastic lesion on the glans penis and coronal sulcus. He was treated 5% 5-fluorouracil cream under occlusion twice a day for 10 days. Three months after treatment, no erythroplastic lesion was found and rebiopsy showed marked improvement histopathologically.

A Case of Porokeratosis Plantaris Plamaris et Disseminata / 대한피부과학회지

Porokeratosis plantaris palmaris et disseminata is a rare variant of porokeratosis. A 37-year-old patient has been affected for about 2 years by mildly discornforting numerous, 1-3mm sized, keratotic lesions of the trunk and limbs, especially on the palms and soles. Histopathologically the diagnosis of porokeratosis was established by demonstration of a cornoid larnella. The family tree of the patient is compatible with autosomal dominant inheritance. The patient has been treated with topical 5-fluorouracil with little auccess.