Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 107
Filter
1.
Article in Chinese | WPRIM | ID: wpr-862544

ABSTRACT

Objective To investigate the noninvasive indicators of indications for antiviral therapy in HBeAg-negative chronic hepatitis B virus (HBV) infection patients with alanine aminotransferase (ALT) ≤40 U/L under the guidance of liver pathology. MethodsA retrospective analysis was performed for the clinical data of 377 HBeAg-negative chronic HBV infection patients with ALT ≤40 U/L who were hospitalized in Affiliated Hospital of Yan’an University, from October 2013 to August 2018 and underwent liver biopsy, among whom the patients with inflammatory activity <A2 and fibrosis stage <F2 were enrolled as non-antiviral therapy group(n=266), and the patients with inflammatory activity ≥A2 or fibrosis stage ≥F2 were enrolled as antiviral therapy group(n=111). The chi-square test was used for comparison of categorical data between two groups; the t-test was used for comparison of normally distributed continuous data between two groups, and the Mann-Whitney U test was used for comparison of non-normally distributed continuous data between two groups; univariate and multivariate binary logistic regression analyses were used to screen out the influencing factors for the initiation of antiviral therapy; the receiver operating characteristic (ROC) curve was plotted to evaluate the diagnostic efficiency of each indicator in determining the need for antiviral therapy in HBeAg-negative chronic HBV infection patients with ALT ≤40 U/L. ResultsOf all 377 patients, 266 (70.6%) did not need antiviral therapy for the time being, and 111 (29.4%) had marked liver damage and thus needed active antiviral therapy. The multivariate analysis showed that liver stiffness measurement (LSM) (odds ratio [HR]=2.003, 95% confidence interval [CI]: 1.647-2.437, P<005), HBsAg (HR=1.563, 95% CI: 1.110-2.200, P<0.05), HBV DNA (HR=1.519, 95% CI: 1173-1.966, P<0.05), and albumin (HR=0.939, 95% CI: 0.884-0.998, P<0.05) were independent influencing factors for the initiation of antiviral therapy. The ROC curve analysis showed that the area under the ROC curve (AUC) was 0.749 (95% CI: 0.699-0799) for LSM, 0642 (95% CI: 0.586-0.699) for HBV DNA, and 0.565 (95% CI: 0.507-0.623) for HBsAg, and the combination of LSM, HBV DNA, and HBsAg had a larger AUC of 0.779 (95% CI: 0.732-0.827). ConclusionThe levels of LSM, HBV DNA, and HBsAg have a reference value in determining the initiation of antiviral therapy in HBeAg-negative chronic HBV infection patients with ALT≤40 U/L.

2.
Acta Pharmaceutica Sinica B ; (6): 3806-3819, 2021.
Article in English | WPRIM | ID: wpr-922442

ABSTRACT

Dioxin-like molecules have been associated with endocrine disruption and liver disease. To better understand aryl hydrocarbon receptor (AHR) biology, metabolic phenotyping and liver proteomics were performed in mice following ligand-activation or whole-body genetic ablation of this receptor. Male wild type (WT) and

3.
Article in Chinese | WPRIM | ID: wpr-910893

ABSTRACT

Objective:To explore the necessity of lowering the cut-off value of alanine aminotransferase (ALT) in identifying chronic HBV infection patients with significant liver histological changes.Methods:The clinical data of 123 chronic HBV infection patients with normal ALT according to domestic criteria who underwent liver biopsy in the Department of Hepatology of Taizhou People’s Hospital from June 2016 to March 2021 were analyzed retrospectively. According to the cut-off ALT values recommended by 2018 version of AASLD guidelines (male 35 U/L, female 25 U/L), the patients were divided into two groups: high normal value group (HNALT, ALT≥AASLD and<domestic standard) and normal low value group (LNALT, ALT<AASLD value). The cases with significant liver histological changes (G/S≥2) were compared between the two groups. Univariate and multivariate Logistic regression analysis were used to explore the risk factors of G/S≥2.Results:There were 68(68/123, 55.3%) cases with significant liver histological changes (G/S≥2) in this series. Among 83 patients in the LNALT group, there were 35 case of G≥2 (42.2%), 26 cases of S≥2 (31.3%) and 39 cases of G/S≥2 (47.0%); while among 40 patients in the HNALT group, there were 27 cases of G≥2 (67.5%), 21 cases of S≥2 (52.5%), and 29 cases of G/S≥2 (72.5%), respectively. There were significant differences between the two groups ( χ2=6.928, 5.126 and 7.107, all P values <0.05). Univariate analysis showed that ALT at high normal values ( χ2=7.107), albumin levels ( t=2.248), glutamyltransferase ( Z=-2.885) and international normalized ratio (INR) ( t=-3.152) were significantly associated with liver histological changes in patients ( P<0.05 ro <0.01). Multivariate Logistic regression analysis showed that ALT at high normal value ( OR=3.492, 95% CI 1.369-8.907) and INR ( OR=1.529, 95% CI 1.054-2.218) were independent risk factors of significant liver histological changes. Conclusion:Lowering the cut-off value of ALT may contribute to identify patients who potentially need antiviral treatment. It is recommended that patients with high normal value of ALT according to domestic criteria should undergo liver biopsy or non-invasive liver fibrosis examination to evaluate the histological changes of the liver and treat them if necessary.

4.
Acta Pharmaceutica Sinica B ; (6): 3244-3261, 2021.
Article in English | WPRIM | ID: wpr-922791

ABSTRACT

Major challenges for cancer treatment are how to effectively eliminate primary tumor and sufficiently induce immunogenic cell death (ICD) to provoke a robust immune response for metastasis control. Here, a self-assembled cascade bioreactor was developed to improve cancer treatment with enhanced tumor penetration and synergistic therapy of starvation, chemodynamic (CDT) and photothermal therapy. Ultrasmall FeS-GOx nanodots were synthesized with glucose oxidase (GOx) as template and induced by paclitaxel (PTX) to form self-assembling FeS-GOx@PTX (FGP)

5.
Article | IMSEAR | ID: sea-194634

ABSTRACT

Background: Alcohol is one of the most common etiology for chronic liver disease. There are several enzymes which remain elevated in both excessive Alcohol consumption and Alcohol induced liver cirrhosis1. But none is sensitive or specific. The ratio of Aspartate transaminase (AST) with Alanine transaminase (ALT) is one of the best marker for alcohol liver disease. Current study mainly compares the ratio of AST/ALT with both Alcoholic liver disease and excessive Alcohol consumption patients.Methods: Observational, cross sectional study conducted on 50 patients diagnosed with alcoholic liver disease and 50 patients of alcohol withdrawal syndrome. Either admitted or seen on outpatient basis at Bangalore medical college and research institute and data was compared among the groups and appropriate statistical methods are applied.Results: The mean ratio of AST/ALT ratio in 50 patients of alcoholic liver disease group was 3.45, whereas the mean ratio in 50 patient of alcohol withdrawal was about 99. When compared statistically this ratio was significant in chronic liver disease group.Conclusions: Most of the patients with heavy alcohol drinking had high AST and alt levels. But ratio of AST/ALT levels was significant high and suggest chronic liver disease secondary to alcohol.

6.
Article | IMSEAR | ID: sea-194627

ABSTRACT

Background: The true prevalence of both NAFLD and NASH are elusive but estimates based on imaging and autopsy studies suggest that about 20-30% of the adults in United States and western countries have excess fat accumulation in the liver. About 10% of these, strictly speaking about 2-3% of the adult population fulfils the criteria of NASH. True prevalence of NAFLD in Indian patients is not known. So, this study was planned to look for current trend of NAFLD in Indian patients.Methods: This prospective observational study was conducted in the Department of Medicine on 65 patients with ultra-sonography finding of fatty liver disease with no history of alcohol, in one year study duration.Results: It is observed that maximum patients are of middle age from age 31-60 years comprising 76% of patients. Out of total patients, 34% and 66% were males and females respectively. Out of 65 patients, 45(69%) had obesity and maximum number of the patients had waist hip ratio and neck circumference more than the cut off value. Out of 65 patients, 19(29%) had hypercholesterolemia and 42(65%) had hyper-triglyceridemia. Out of 65 patients, 32(49%) had higher alanine transaminase (ALT) level and 17(29%) patients had higher AST level. Out of 65 patients, 29(45%) had the homeostasis model assessment of insulin resistance (HOMA-IR) less than cut off value (less than 2.25) and remaining 36(55%) were having HOMA-IR more than 2.25. The sensitivity for the cut off value for HOMA-IR is 72.7% and specificity is 49.1%.Conclusions: Obesity, neck circumference, and waist hip ratio are higher than its cut off value for both sex, insulin resistance evaluated through HOMA- IR directly relates to the causation of NAFLD but at some extents higher triglyceride levels are also associated but the values of ALT and AST levels did not give any clue in these cases of NAFLD.

7.
Article in Chinese | WPRIM | ID: wpr-873194

ABSTRACT

Objective:To study the protective effect of Ficus pandurata extract on acute alcoholic liver injury based on pyroptosis mechanism.Method:The 56 male C57BL/6 mice were randomly divided into normal control group, model control group, positive control group(60 mg·kg-1), fresh medicine water extract group(48 g·kg-1), dry drug water extract group(48 g·kg-1),dry drug 50% alcohol extract group(48 g·kg-1) and dry drug 95% alcohol extract group (48 g·kg-1), 8 mice in each group.Positive control and different solvent extract groups of Ficus tenuifolia were intragastrically administrated for 18 days,once a day,while normal group and model group were given the same volume of pure water intragastrically. After 15 days of continuous gavage, mice received 50% ethanol(12 mL·kg-1)intragastrically for 3 days to induce acute alcoholic liver injury model except for the normal control group. At 14 h after the last treatment,serum and liver samples were obtained,the serum content of alanine aminotransferase (ALT) and aspartate transaminase(AST) were determined, the histopathologic changes of the hepatic tissues were observed by hematoxylin ecosin(HE) staining.The content of malondialdehyde (MDA) in liver was determined by thiobarbituric acid (TBA) and the content of lactate dehydrogenase (LDH) was determined by microplate method. Western blot and TUNEL assay kit was used to detect the cell pyroptosis rate.Result:Compared with normal group, ALT, AST, MDA and LDH levels in the model group were significantly increased, liver index was significantly increased,TUNEL staining positive, inflammatory factors and pyroptosis related protein expressions were significantly increased (P<0.05). Compared with model control group, the ALT,AST ,MDA and LDH of the drug intervention group decreased significantly (P<0.05). The liver index decreased in different degrees, and the expression of inflammatory factors and pyroptosis related protein in the water extract treatment group decreased significantly (P<0.05).Conclusion:The root extract of Ficus pandurata Hance has protective effect on acute alcoholic liver injury, and the mechanism of water extract might relate to inhibiting hepatocyte pyroptosis.

8.
Article in Chinese | WPRIM | ID: wpr-870405

ABSTRACT

Objective To study the relationship between preoperative serum ALT and clinicopathological factors in patients with gastric cancer after radical gastrectomy.Methods At the Department of Gastrointestinal Surgery,Tumor Hospital of Harbin Medical University from Jan 2008 to Dec 2010,491 patients were grouped according to ROC curve cut-off point of serum ALT.The relationship between ALT and clinical factors was analyzed,and single-factor and multi-factor survival analysis was performed.Results There were 201 patients with ALT ≤ 20.05 U/L,and 290 patients with ALT > 20.05 U/L Serum ALT was associated with age (x2 =11.231,P < 0.001),depth of tumor invasion (x2 =23.178,P<0.001),GGT(x2 =19.190,P<0.001) and AST(x2 =30.771,P<0.001).The 1-,3-,and 5-year survival rates of patients with ALT ≤ 20.05U/L and ALT > 20.05U/L were 83.5%,51.4%,42.1% and 66.2%,27.4%,15.7%.There was significant difference between the two groups (x2 =41.711,P<0.001).Muhivariate analysis showed that tumor TNM stage(HR =1.882,95% CI:1.468-2.413,P <0.001),tumor infiltration depth (HR =1.161,95% CI:1.020-1.322,P =0.024),lymph node metastasis (HR =1.177,95% CI:1.042-1.329,P =0.009),Hb (HR =0.726,95% CI:0.579-0.909,P =0.005),neutrophil to lymphocyte rate(HR =1.275,95% CI:1.002-1.623,P =0.048) and ALT(HR =2.191,95% CI:1.754-2.738,P < 0.001) were independent risk factors for the prognosis.Conclusions Serum ALT is an independent risk factor for the prognosis of gastric cancer patients after radical gastrectomy.

9.
Journal of Clinical Hepatology ; (12): 2446-2449, 2020.
Article in Chinese | WPRIM | ID: wpr-829630

ABSTRACT

ObjectiveTo investigate the clinical significance of highly sensitive nucleic acid detection in precise antiviral therapy for patients with liver cirrhosis and its association with aminotransferase level. MethodsA total of 377 patients with hepatitis B cirrhosis who were hospitalized or attended the outpatient service from May 2013 to April 2019 were enrolled and tested by both domestic HBV DNA detection and highly sensitive Cobas HBV DNA detection. All patients underwent biochemical examination, four blood coagulation tests, routine blood test, and upper abdominal computed tomography or ultrasound. Sensitivity of different HBV DNA detection reagents was compared in liver cirrhosis patients with a low viral load, and the correlation between alanine aminotransferase (ALT) level and viral load was analyzed. The paired t-test was used for comparison of continuous data before and after treatment. The receiver operating characteristic (ROC) curve was used to screen out the optimal predictive values of ALT at different cut-off values of HBV DNA. ResultsAmong the 377 patients with hepatitis B cirrhosis, 215 tested positive and 162 tested negative by domestic HBV DNA, and among these 162 patients, 104 (64.2%) tested positive by Cobas HBV DNA detection, with a mean level of 267.5±42.3 IU/ml. After 24 weeks of antiviral therapy, the 104 patients with hepatitis B cirrhosis had significant improvements in viral replication level, ALT, and Child-Pugh score for liver function; HBV DNA decreased from 267.5±32.2 IU/ml before treatment to 59.6±7.7 IU/ml after treatment (t=3.486, P=0.002), ALT decreased from 871±10.8 U/L before treatment to 36.5±7.6 U/L after treatment (t=3.235, P=0.020), and the Child-Pugh score decreased from 6.5±0.7 before treatment to 5.7±0.5 after treatment (t=2.928, P=0.041). The ROC curve analysis of ALT in predicting HBV DNA decision point showed that an ALT level of 29 IU/L was the most sensitive cut-off value for predicting HBV DNA <20 IU/ml, with an area under the ROC curve of 0.904, a sensitivity of 1.0, and a specificity of 0.237. ConclusionPrecise detection helps to guarantee the precise clinical treatment of patients with hepatitis B cirrhosis and improve their treatment outcome and prognosis. An ALT level of 29 IU/L is a sensitive indicator for predicting patients with negative Cobas HBV DNA, so as to achieve individualized precise screening and treatment.

10.
Acta méd. peru ; 36(4): 253-258, oct.-dic 2019. ilus, tab
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1141955

ABSTRACT

Objetivo: Evaluar la alanina aminotransferasa (ALT) como marcador en el diagnóstico de síndrome metabólico (SM) y riesgo cardiovascular (RCV) en niños con obesidad exógena. Materiales y métodos: Estudio transversal. Se incluyeron niños con obesidad exógena de 2 a 14 años, atendidos en la Unidad de Endocrinología Pediátrica del Hospital Nacional Cayetano Heredia, entre el 2014 al 2018. Se definió enfermedad hepática no alcohólica (EHNA) considerando dos puntos de corte de ALT; en mujeres: >22,1U/L y >44U/L, en varones: >25,8U/L y >50U/L. Se definió SM según la Academia Americana de Pediatría y RCV con TG/HDL-C ≥3,5. Aplicamos Chi cuadrado, considerándose significativo p<0,05. Estimamos sensibilidad(S), especificidad (E) y likelihood ratios (LR). Resultados: Se incluyeron 347 niños obesos (54,7% varones). La frecuencia de EHNA fue de 23,1%. La sensibilidad y especificidad para el diagnóstico de SM con ALT >22,1U/L y >25,8U/L fue 79,4% y 37,6% respectivamente y, con ALT>44U/L y >50U/L fue 28,6% y 83,3%. La ALT con punto de corte mayor en conjunto con TG/HDL-C≥3,5 mostró una especificidad del 96,9% y un likelihood ratio + (LR+) de 6,7. Conclusión: La ALT con un punto de corte >44U/L en mujeres y >50U/L en varones, es un marcador bioquímico útil en la identificación de SM y riesgo cardiovascular en niños con obesidad exógena desde los primeros años de vida.


Objective: To assess alanine aminotransferase (ALT) as a marker for diagnosing metabolic syndrome (MS) and cardiovascular risk in children with exogenous obesity. Materials and Methods: This is a cross-sectional study. Children with exogenous obesity between 2 and 14 years of age seen in the Pediatric Endocrinology Unit at Cayetano Heredia Hospital between 2014 and 2018 were included. Non alcoholic liver disease was defined considering two ALT cutoff points: >22.1 U/L and >44 U/L in females and >25.8 U/L and >50 U/L in males. MS was defined according to the American Academy of Pediatrics criteria, and cardiovascular risk was defined with TG/HDL-C ratio ³3.5. We used chi-square test, and p<0.05 was deemed as significant. We estimated sensitivity (S), specificity (E) and likelihood ratios (LR). Results: Three-hundred and forty-seven obese children were included (54.7% were male). Non-alcoholic liver disease frequency was 23.1%. Sensitivity and specificity values for diagnosing MS with ALT >22.1 U/L and >25.8 U/L were 79.4% and 37.6%, respectively; and with ALT >44 U/L and 50 U/L these values were 28.6% and 83.3%. ALT levels with higher cutoff values, together with a ³3.5 TG/HDL-C ratio showed 96.9% specificity and 6.7 likelihood ratio (LR+). Conclusion: ALT levels with a >44 U/L cutoff value in females and >50 U/L in males is a useful biochemical marker for identifying MS and determining cardiovascular risk in children with exogen obesity during their early years of life.

11.
Article | IMSEAR | ID: sea-202594

ABSTRACT

Introduction: Alcohol is consumed all over the world. Itis toxic to liver. It causes different liver problems, like fattyliver, alcoholic hepatitis and cirrhosis. The disorders causedby alcohol use are main causes of mortality and morbidity.Excessive consumption of alcohol is one of the top 5 riskfactors for death and disability globally. The present studywas conducted to diagnose alcoholic liver diseases so thatby proper approach patient can be brought to normal life.Material and methods: This prospective observational studycomprising of 50 patients was conducted at SHKM Govt.Medical College, Nalhar, Haryana from April 2018 to March2019. The detail history, Audit scoring, physical examinations,lab investigations and ultrasound studies were done. Properethical norms were maintained and statistical analysis wascarried out.Results: Out of total of 50 patients, 12% patients wereasymptomatic, 68% patients had fatty liver on the basis ofultrasonography and AST and ALT levels. 12% patients hadalcoholic hepatitis and 8% patients had cirrhosis. AST andALT were raised in most patients. AUDIT score was morethan 8 in all patients of alcoholic hepatitis and cirrhosis.Discussion: Alcoholic liver disease affects only smallpercentage of regular drinkers. Since alcoholic liver diseasein most of the patients is potentially reversible, hence afterproper diagnosis and with a sober approach, regular efforts,psychological counselling and use of pharmacological agentswe can treat the patients of alcoholic liver disease and bringthem to the normal life, which is the aim of this study.

12.
Acta cir. bras ; 34(6): e201900607, 2019. tab, graf
Article in English | LILACS | ID: biblio-1019264

ABSTRACT

Abstract Purpose Coleus forskohlii Briq., a medicinal plant originally from India, has been indicated against heart disease, expiratory disorders, convulsions, and hepatic changes, among others. In view of the broad pharmacological potential of the plant and the scarce information about its effects, the objective of the present study was to investigate the effect of its use for pretreatment of partially hepatectomized rats. Methods The animals were divided into two experimental groups: Control (CG) receiving physiological saline for 10 days before partial hepatetctomy, and Treated (TG) receiving 40 mg Coleus forskohlii/kg/day for 10 days before partial hepatectomy. The treatments were performed by gastric gavage. After the surgical procedure, treatment was continued according to the following groups: CG 24 h, CG 48 h, TG 24 h, and TG 48 hs, and liver tissue and intracardiac blood samples were obtained for histological and biochemical analysis, respectively. Results No significant differences were observed in mitotic or apoptotic index or in the concentrations of the enzymes AST, ALT and alkaline phosphatase, and no areas of fibrosis were detected. Conclusion Treatment with Coleus forskohlii did not interfere with the course of hepatic hyperplasia.


Subject(s)
Animals , Male , Rats , Plant Extracts/administration & dosage , Plectranthus/chemistry , Hepatectomy/methods , Liver/pathology , Aspartate Aminotransferases/blood , Biomarkers/blood , Hepatocytes/drug effects , Alanine Transaminase/blood , Alkaline Phosphatase/blood , Hyperplasia/drug therapy , Liver/surgery , Liver/drug effects
13.
Article in English | WPRIM | ID: wpr-761557

ABSTRACT

BACKGROUND/AIMS: The serum aminotransferase level is usually elevated in rhabdomyolysis, and these enzymes originate from the skeletal muscle. On the other hand, there is limited data showing whether the degree of elevation of these enzymes differs according to the concurrent liver disease. METHODS: Patients with rhabdomyolysis were selected when their serum creatinine kinase level was >1,000 U/L. They were categorized as the group with and without concurrent liver disease. The AST and ALT levels in both groups were compared. In addition, the aminotransferase level was compared between those with rhabdomyolysis and those with alcoholic liver disease. RESULTS: Among the 165 patients with rhabdomyolysis, 19 had concurrent liver disease. The median peak AST was higher in the group with concurrent liver disease (332 U/L [interquartile range (IQR), 127–1,604] vs. 219 U/L [IQR, 115–504]). In addition, the median peak ALT was higher in the group with concurrent liver disease (107 U/L [IQR, 74–418] vs. 101 U/L [IQR, 56–218]). On the other hand, there was no significant difference in both enzymes between the two groups. The median peak AST level was significantly higher in those with rhabdomyolysis than in those with alcoholic liver disease (221 U/L [IQR, 118–553] vs. 103 U/L [IQR, 59–206]), but the median peak ALT was not significantly different (102 U/L [IQR, 58–222] vs. 51 U/L [IQR, 26–117]). CONCLUSIONS: Rhabdomyolysis showed an elevated AST-dominant aminotransferase level, which is not different according to concurrent liver disease. Therefore, it is recommended that rhabdomyolysis be considered first in cases of elevated aminotransferase levels in patients with a suspicious skeletal muscle injury.


Subject(s)
Alanine Transaminase , Aspartate Aminotransferases , Creatinine , Hand , Humans , Liver Diseases , Liver Diseases, Alcoholic , Liver , Muscle, Skeletal , Phosphotransferases , Rhabdomyolysis
14.
Acta Pharmaceutica Sinica B ; (6): 421-432, 2019.
Article in English | WPRIM | ID: wpr-774977

ABSTRACT

Prodrug nanoassemblies, which can refrain from large excipients, achieve higher drug loading and control drug release, have been placed as the priority in drug delivery system. Reasoning that glutathione (GSH) and reactive oxygen species (ROS) are highly upgraded in tumor tissues which makes them attractive targets for drug delivery system, we designed and synthetized a novel prodrug which utilized mono thioether bond as a linker to bridge linoleic acid (LA) and docetaxel (DTX). This mono thioether-linked conjugates (DTX-S-LA) could self-assemble into nanoparticles without the aid of much excipients. The mono thioether endowed the nanoparticles redox sensitivity resulting in specific release at the tumor tissue. Our studies demonstrated that the nanoassemblies had uniform particle size, high stability and fast release behavior. DTX-S-LA nanoassemblies outperformed DTX solution in pharmacokinetic profiles for it had longer circulation time and higher area under curve (AUC). Compared with DTX solution, the redox dual-responsive nanoassemblies had comparable cytotoxic activity. Besides, the antitumor efficacy was evaluated in mice bearing 4T1 xenograft. It turned out this nanoassemblies could enhance anticancer efficacy by increasing the dose because of higher tolerance. Overall, these results indicated that the redox sensitivity nanoassemblies may have a great potential to cancer therapy.

15.
Acta Pharmaceutica Sinica B ; (6): 1050-1060, 2019.
Article in English | WPRIM | ID: wpr-774923

ABSTRACT

Chemotherapy is among the limited choices approved for the treatment of hepatocellular carcinoma (HCC) at intermediate and advanced stages. Preferential and prolonged drug exposure in diseased sites is required to maximize the therapeutic index of the drug. Here, we report an injectable supramolecular peptide hydrogel as an intraperitoneal depot for localized and sustained release of triptolide for the treatment of orthotopic HCC. We chose peptide amphiphile C-GNNQQNYKD-OH-based nanofibers as gelators and carriers for triptolide. Sustained triptolide release from the hydrogel was achieved over 14 days , with higher accumulation in and cytotoxicity against human HCC Bel-7402 in comparison with L-02 fetal hepatocytes. After intraperitoneal injection, the hydrogel showed prolonged retention over 13 days and preferential accumulation in the liver, realizing HCC growth inhibition by 99.7 ± 0.1% and animal median survival extension from 19 to 43 days, without causing noticeable pathological changes in the major organs. These results demonstrate that injectable peptide hydrogel can be a potential carrier for localized chemotherapy of HCC.

16.
Article in Chinese | WPRIM | ID: wpr-798138

ABSTRACT

Objective@#To explore the clinical value of alanine aminotransferase(ALT), aspartate aminotransferase(AST) and gamma-glutamine transpeptidase(GGT) in the diagnosis of hepatitis B-related hepatocellular carcinoma, and to provide reference for clinical diagnosis.@*Methods@#From November 2015 to November 2017, 118 patients with hepatitis B-related primary liver cancer (hepatitis B-related primary liver cancer group) admitted to Tongji Huangzhou Hospital of Huazhong University of Science and Technology were selected.And 116 patients with cirrhosis (hepatocirrhosis group) and 114 healthy people were selected as control group.The levels of ALT, AST and GGT and the specificity and sensitivity of each index were compared among the three groups, and the ROC curve was analyzed.@*Results@#The levels of serum ALT, AST and GGT in the hepatitis B-related primary hepatocellular carcinoma group were higher than those in the cirrhosis group and the control group, and the above serum indicators in the cirrhosis group were higher than those in the control group[(267.1±131.5)U/L vs.(31.2±11.3)U/L vs.(17.4±4.6)U/L, (76.6±23.2)U/L vs.(45.2±13.1)U/L vs.(24.3±6.6)U/L, (125.3±42.6)U/L vs.(53.1±17.6)U/L vs.(22.4±4.4)U/L, all P<0.05]. ROC curve showed that the area under GGT curve was 0.85, under ALT curve was 0.78, and under AST curve was 0.73, the area under the combined diagnostic curve of the three indicators was 0.95.The sensitivity and specificity of three combined diagnosis were higher than those of ALT, AST and GGT.@*Conclusion@#The levels of serum ALT, AST and GGT in patients with hepatitis B-related primary liver cancer are elevated, and the combined diagnosis of the three indicators has high sensitivity and specificity, which is worthy of clinical reference.

17.
Article in Chinese | WPRIM | ID: wpr-805293

ABSTRACT

Objective@#To investigate the expression and clinical significance of ribonucleotide reductase small subunit M2 (RRM2) in chronic hepatitis B virus (HBV) infection and related liver diseases.@*Methods@#A total of 428 patients with chronic HBV infection and liver disease were enrolled from Songyang County People’s Hospital from October 2017 to September 2019. There were 166 cases of chronic hepatitis B (CHB), 53 cases of HBV-related cirrhosis, 28 cases of non-HBV-related cirrhosis, 57 cases of HBV-related liver cancer, 33 cases of non-HBV-related liver cancer, and 91 cases of non-viral hepatitis. In addition, 36 healthy subjects were selected as the control group. Among 166 cases of CHB, there were 87 patients with high viral load group (HBV DNA ≥4.0 lg IU/mL) and 79 patients with low viral load group (HBV DNA <4.0 lg IU/mL); while in 87 high viral load patients, 56 had high alanine transaminase (ALT) (≥40 U/L) and 31 had normal ALT (<40 U/L). The expression level of serum RRM2 protein in patients was detected by enzyme-linked immunosorbent assay (ELISA), and the relationship of RRM2 expression with HBV DNA and liver function was analyzed. SPSS 23.0 and PRISM 8.0 statistical software were used to analyze data. Correlation analysis was performed using Spearman analysis.@*Results@#The serum ALT and RRM2 levels in patients with high viral load CHB were higher than those in low viral load group (Z=-6.68, t=6.80, P<0.01). Patients with HBV-related cirrhosis had higher serum RRM2 levels than those with non-HBV-related cirrhosis (t=9.16, P<0.01). The serum RRM2 level was higher in patients with HBV-related liver cancer than that in patients with non-HBV-related liver cancer (t=12.42, P<0.01). Among patients with high viral load CHB, there was no significant difference in serum RRM2 levels between patients with ALT ≥40 U/L group and patients with ALT <40 U/L group (t=0.51, P>0.05). The level of ALT in the non-viral hepatitis group was higher than that in the healthy control group (Z=-8.43, P<0.01), but there was no significant difference in serum RRM2 levels between the two groups (t=1.03, P>0.05). Correlation analysis showed that serum RRM2 level was positively correlated with HBV DNA load (r=0.51, P<0.01), but not correlated with liver function indicators such as ALT and aspartate aminotransferase (all P>0.05) in patients with chronic HBV infection and related liver diseases.@*Conclusions@#Serum RRM2 level is positively correlated with HBV DNA load and has no significant correlation with ALT. RRM2 might be used as a target for the development of new hepatitis B drugs.

18.
Article in Chinese | WPRIM | ID: wpr-744506

ABSTRACT

Objective To explore the clinical value of alanine aminotransferase (ALT),aspartate aminotransferase(AST) and gamma-glutamine transpeptidase(GGT) in the diagnosis of hepatitis B-related hepatocellular carcinoma,and to provide reference for clinical diagnosis.Methods From November 2015 to November 2017,118 patients with hepatitis B-related primary liver cancer (hepatitis B-related primary liver cancer group) admitted to Tongji Huangzhou Hospital of Huazhong University of Science and Technology were selected.And 116 patients with cirrhosis (hepatocirrhosis group) and 114 healthy people were selected as control group.The levels of ALT,AST and GGT and the specificity and sensitivity of each index were compared among the three groups,and the ROC curve was analyzed.Results The levels of serum ALT,AST and GGT in the hepatitis B-related primary hepatocellular carcinoma group were higher than those in the cirrhosis group and the control group,and the above serum indicators in the cirrhosis group were higher than those in the control group [(267.1 ± 131.5) U/L vs.(31.2 ± 11.3) U/L vs.(17.4 ± 4.6) U/L,(76.6 ± 23.2) U/L vs.(45.2 ± 13.1) U/L vs.(24.3 ± 6.6) U/L,(125.3 ± 42.6) U/L vs.(53.1 ± 17.6)U/L vs.(22.4 ± 4.4)U/L,all P < 0.05].ROC curve showed that the area under GGT curve was 0.85,under ALT curve was 0.78,and under AST curve was 0.73,the area under the combined diagnostic curve of the three indicators was 0.95.The sensitivity and specificity of three combined diagnosis were higher than those of ALT,AST and GGT.Conclusion The levels of serum ALT,AST and GGT in patients with hepatitis B-related primary liver cancer are elevated,and the combined diagnosis of the three indicators has high sensitivity and specificity,which is worthy of clinical reference.

19.
Article in English | WPRIM | ID: wpr-741841

ABSTRACT

PURPOSE: Vitamin D deficiency is a condition widespread throughout the world. Recent studies have suggested that vitamin D deficiency was associated with obesity and metabolic syndrome. The purpose of the study was to examine the relationship between vitamin D deficiency and nonalcoholic fatty liver disease (NAFLD) in adolescents. METHODS: The data were obtained from the Korean National Health and Nutrition Examination Survey from 2008–2014. A total of 3,878 adolescents were included in the study. Vitamin D deficiency was defined as a 25-hydroxyvitamin D concentration 30 U/L. RESULTS: Vitamin D deficiency was noted in 78.9% of the studied population. Age, body mass index, waist circumference, and blood pressure, glucose, cholesterol, and triglyceride levels were significantly higher in adolescents with suspected NAFLD than in adolescents without suspected NAFLD, while the mean vitamin D level was significantly lower in adolescents with suspected NAFLD. The multivariate-adjusted odds of suspected NAFLD were higher with increased age, male gender, obesity, and metabolic syndrome. Individuals with vitamin D deficiency were at higher risk of suspected NAFLD (odds ratio, 1.77; 95% confidence interval, 1.07–2.95) after adjusting for age, gender, obesity, and metabolic syndrome. CONCLUSION: Vitamin D deficiency was associated with suspected NAFLD, independent of obesity and metabolic syndrome, in adolescents.


Subject(s)
Adolescent , Alanine Transaminase , Blood Pressure , Body Mass Index , Child , Cholesterol , Glucose , Humans , Male , Non-alcoholic Fatty Liver Disease , Nutrition Surveys , Obesity , Triglycerides , Vitamin D Deficiency , Vitamin D , Vitamins , Waist Circumference
20.
Article in English | WPRIM | ID: wpr-787205

ABSTRACT

BACKGROUND/AIMS: The serum aminotransferase level is usually elevated in rhabdomyolysis, and these enzymes originate from the skeletal muscle. On the other hand, there is limited data showing whether the degree of elevation of these enzymes differs according to the concurrent liver disease.METHODS: Patients with rhabdomyolysis were selected when their serum creatinine kinase level was >1,000 U/L. They were categorized as the group with and without concurrent liver disease. The AST and ALT levels in both groups were compared. In addition, the aminotransferase level was compared between those with rhabdomyolysis and those with alcoholic liver disease.RESULTS: Among the 165 patients with rhabdomyolysis, 19 had concurrent liver disease. The median peak AST was higher in the group with concurrent liver disease (332 U/L [interquartile range (IQR), 127–1,604] vs. 219 U/L [IQR, 115–504]). In addition, the median peak ALT was higher in the group with concurrent liver disease (107 U/L [IQR, 74–418] vs. 101 U/L [IQR, 56–218]). On the other hand, there was no significant difference in both enzymes between the two groups. The median peak AST level was significantly higher in those with rhabdomyolysis than in those with alcoholic liver disease (221 U/L [IQR, 118–553] vs. 103 U/L [IQR, 59–206]), but the median peak ALT was not significantly different (102 U/L [IQR, 58–222] vs. 51 U/L [IQR, 26–117]).CONCLUSIONS: Rhabdomyolysis showed an elevated AST-dominant aminotransferase level, which is not different according to concurrent liver disease. Therefore, it is recommended that rhabdomyolysis be considered first in cases of elevated aminotransferase levels in patients with a suspicious skeletal muscle injury.


Subject(s)
Alanine Transaminase , Aspartate Aminotransferases , Creatinine , Hand , Humans , Liver Diseases , Liver Diseases, Alcoholic , Liver , Muscle, Skeletal , Phosphotransferases , Rhabdomyolysis
SELECTION OF CITATIONS
SEARCH DETAIL