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1.
Article in Chinese | WPRIM | ID: wpr-847128

ABSTRACT

BACKGROUND: Heterotopic ossification is a common complication of acetabular fracture. Its pathogenesis is still not completely clear, and it is mainly caused by skeletal muscle trauma, hip surgery or nervous system injury. From the research in the past 10 years, the risk factors for ectopic ossification after acetabular fracture surgery are numerous, but its prevention and treatment are controversial. OBJECTIVE: To summarize the relevant literature on the diagnosis, prevention and treatment of ectopic ossification after acetabular fracture surgery in recent years and the risk factors that may affect the prognosis, so as to provide a basis for the clinical prevention and treatment of ectopic ossification after acetabular fracture surgery. METHODS: The databases of Wanfang Medical Network, CNKI and PubMed were searched using computer. The key words were “heteropic ossification; acetabular fractures surgery; NSAIDs; risk factors; radiotherapy; treatment” in Chinese and English. According to the inclusion and exclusion criteria, 67 related articles were finally included and summarized. RESULTS AND CONCLUSION: (1) Although the etiology of heterotopic ossification is not fully understood, it is generally accepted that the formation of heterotopic ossification is determined by both systemic factors and local tissue changes. The risk factors of heterotopic ossification are various, and some preventive measures should be taken for high-risk patients. (2) The prevention of heterotopic ossification after acetabular fracture surgery mainly includes drug, radiotherapy and combined prevention, among which drug prevention, especially non-steroidal anti-inflammatory drugs, is still the most economical and effective prevention method. (3) The treatment of heterotopic ossification is mainly ectopic bone resection and hip arthroplasty. The corresponding treatment methods based on the biomolecular mechanism are still in the stage of animal experimental research, but it points out the direction for the treatment of heterotopic ossification. (4) Among the existing treatment methods, surgery is recognized as the most effective treatment.

2.
International Eye Science ; (12): 450-454, 2021.
Article in Chinese | WPRIM | ID: wpr-873442

ABSTRACT

@#Physical exercise is a low-cost, easy-to-implement therapeutic intervention. In recent years, it has been found that exercise has a protective effect on retinal diseases such as diabetic retinopathy, age-related macular degeneration and retinitis pigmentosa. The mechanism may be related to enhancing retinal defence against oxidative stress, reducing glutamate secretion and inhibiting retinal cell apoptosis through BDNF/TrkB pathway. This paper reviews the recent literature related to the protective effect of exercise on retina from animal experiments, clinical studies and mechanism investigation.

3.
Article in Chinese | WPRIM | ID: wpr-906527

ABSTRACT

Objective:To review and summarize the current research status of traditional Chinese medicine(TCM) for the treatment of chronic atrophic gastritis(CAG),provide references and hints for relevant studies,and contribute to the further understanding of TCM and the application of TCM in the treatment of CAG with scientific evidence. Method:The PubMed and Web of Science databases were searched for relevant literature on the treatment of CAG with TCM from their establishment to August 31,2020. Eligible randomized controlled trials (RCTs) and animal studies were included according to the inclusion and exclusion criteria,and then the information of the included studies was extracted,summarized,and organized for further analysis. Result:A total of 4 RCTs and 21 animal studies (including 13 papers on compound studies,3 papers on single herb studies,and 5 papers on monomer studies) about TCM treatment for CAG were included in this study. RCTs showed that TCM could work well in improving the pathological state of gastric mucosa and clinical symptoms in patients. However,there were problems of low study quality,and non-uniform diagnostic criteria for gastric mucosal pathology and clinical efficiency evaluation. Animal experiments mainly focused on the study of drug mechanism exploration,and their results showed that TCM treatment of CAG was characterized by multi-target action. However,the animal experiments also had some problems such as inconsistence of CAG animal model establishment,positive drug selection,drug intervention methods as well as intervention cycles among different experiments. Conclusion:The efficacy of TCM in the treatment of CAG has gradually gained global recognition,but there is still a need for further standardization and unification of research methods. In the future,high-quality clinical trials and standardized animal experiments are still needed to conduct in-depth studies on the time for intervention,intervention methods,active ingredients and mechanisms of TCM,so as to make contributions to the full understanding and application of TCM in the treatment of CAG.

4.
Braz. j. med. biol. res ; 54(4): e10117, 2021. tab, graf
Article in English | LILACS | ID: biblio-1153531

ABSTRACT

The long noncoding RNA (lncRNA) H19 is involved in the pathogenesis of endometriosis by modulating the proliferation and invasion of ectopic endometrial cells in vitro, but related in vivo studies are rare. This study aimed to investigate the role of lncRNA H19 in a nude mouse model of endometriosis. Ectopic endometrial stromal cells (ecESCs) were isolated from ectopic endometrium of patients with endometriosis and infected with lentiviruses expressing short hairpin RNA (shRNA) negative control (LV-NC-shRNA) or lncRNA-H19 shRNA (LV-H19-shRNA). The ecESCs infected with LV-NC-shRNA and LV-H19-shRNA were subcutaneously implanted into forty 6- to 8-week-old female nude mice. The size and weight of the endometriotic implants were measured at 1, 2, 3, and 4 weeks after implantation and compared, and lncRNA H19 levels in endometriotic implants were evaluated using real-time polymerase chain reaction (RT-PCR). All nude mice survived the experimental period, and no significant differences in body weight were observed between the experimental group and the control group. All nude mice developed histologically confirmed subcutaneous endometriotic lesions with glandular structures and stroma after 1 week of implantation. The subcutaneous lesions in the LV-NC-shRNA group after 1, 2, 3, and 4 weeks of implantation were larger than those in the LV-H19-shRNA group, and lncRNA H19 levels in subcutaneous lesions in the LV-NC-shRNA group were significantly higher than those in the LV-H19-shRNA group. Knockdown of lncRNA H19 suppresses endometriosis in vivo. Further study is required to explore the underlying mechanism in the future.


Subject(s)
Humans , Animals , Female , Rabbits , Endometriosis/genetics , RNA, Long Noncoding/genetics , RNA, Small Interfering/genetics , Cell Proliferation/genetics , Endometrium , Mice, Nude
5.
Article in Chinese | WPRIM | ID: wpr-847640

ABSTRACT

BACKGROUND: It has been shown that compared with stem cells from other sources, umbilical cord-derived mesenchymal stem cells have lower immunogenicity, and their application has significant effect in rats with myocardial infarction. OBJECTIVE: To systematically evaluate the effects of umbilical cord-derived mesenchymal stem cells on cardiac function of rats with myocardial infarction. METHODS: PubMed, Cochrane, Embase, CBM, CNKI, WanFang, VIP and CJD databases were retrieved for the literature concerning umbilical cord-derived mesenchymal stem cells for treating rats with myocardial infarction published before June 2019. Two researchers independently completed literature screening, data extraction and methodological quality evaluation according to the inclusion criteria. Meta-analysis was conducted on Stata 14.0. RESULTS AND CONCLUSION: A total of 9 articles were included, involving 216 rats. Meta-analysis showed that: (1) Umbilical cord-derived mesenchymal stem cells significantly increased the left ventricular ejection fraction after myocardial infarction in rats [95% confidence interval (CI) (3.16, 3.76), P < 0.001]. (2) Umbilical cord-derived mesenchymal stem cells significantly increased the left ventricular short axis shortening rate after myocardial infarction in rats [95%CI (0.18, 0.54), P < 0.001]. (3) Umbilical cord-derived mesenchymal stem cells significantly shortened the left ventricular end-diastolic internal diameter [95%CI (-1.90,-0.99), P=0.042] and left ventricular end-systolic internal diameter [95%CI (-6.56,-4.65), P < 0.001]. (4) Umbilical cord-derived mesenchymal stem cells significantly improved the left ventricular end-diastolic volume [95%CI (-2.01,-1.11), P < 0.001] and left ventricular end-systolic volume [95%CI (-3.44,-2.17), P < 0.001]. In summary, umbilical cord-derived mesenchymal stem cell transplantation is effective and safe in the treatment of myocardial infarction in rats. Due to the limitation of the quality of the included literature, the above conclusions need to be validated by high-quality and large-sample randomized controlled trials.

6.
Article | IMSEAR | ID: sea-200101

ABSTRACT

Background: Computer Assisted Learning (CAL) for teaching experimental pharmacology is now widely accepted as alternative to animal experiments. The objective of this study was to compare the performance of undergraduate medical students by conventional teaching methods (lecture and discussion) and CAL.Methods: This was a questionnaire based observational study involving 109 MBBS students of fifth semester. The students were taught experimental pharmacology practical by both conventional methods (lecture with discussion) and computer assisted learning (CAL). Questionnaire and their filled responses by these students were taken at the end of lecture-discussion (pre-CAL) and after the CAL experiments (post-CAL), covering the same topics of experimental pharmacology. Pre-CAL and post-CAL data were assessed statistically.Results: In the pre-CAL session, only 53.39%, 47.56%, 53.39% and 49.5% of the students were having the scores above 40% in the rabbit eye, DRC and potentiation, frog heart and dog BP experiment respectively, which was increased to 77.44%, 75.48%, 75.47% and 75.48% of the students respectively in the post-CAL assessment. A statistically significant difference (p <0.05) in the performance was observed among the students in the pre-CAL and post-CAL assessment.Conclusions: CAL is a good alternative to animal experimentation. Lecture with discussion, followed by CAL experiments on the same topics, enhanced the performance of students as shown by improvement in post-CAL scores.

7.
Article in Chinese | WPRIM | ID: wpr-775928

ABSTRACT

OBJECTIVE@#To systematically review the effectiveness and safety of electroacupuncture (EA) on hyperlipidemia combined with atherosclerosis in animal experiments.@*METHODS@#The relevant animal experiments of EA treatment for hyperlipidemia combined with atherosclerosis were retrieved from the CNKI, WANFANG, VIP, CBM, PubMed, Embase and the Cochrane Library up to March 19, 2018 since the establishment of databases. Two researchers in the project team screened the literature and extracted the information such as experiment objects, experiment steps and experiment results separately. The literature assessment was evaluated. Meta analysis was performed with RevMan 5.3 software.@*RESULTS@#Eventually, 29 eligible experimental researches were included and the average score in the quality assessment was 5.1, at the moderate level. Meta analysis showed that compared with the model control group, after EA treatment, the levels of the total cholesterol (TC) [ -2.29, 95% (-2.94, -1.64), <0.000 01], triglyceride (TG) [ -1.30, 95% (-1.76, -0.84), <0.000 01] and low density lipoprotein-cholesterol (LDL-C) [ -2.64, 95% (-3.29, -1.99), <0.000 01] were significantly reduced and the level of high density lipoprotein-cholesterol (HDL-C) [ 0.55, 95% (0.06, 1.04), =0.03] was increased, indicating the significant differences.@*CONCLUSION@#On the base of the current limited evidences, EA significantly reduces the levels of TC, TG and LDL-C and slightly increases the level of HDL-C in the animal models of hyperlipidemia combined with atherosclerosis.


Subject(s)
Animal Experimentation , Animals , Atherosclerosis , Therapeutics , Cholesterol, LDL , Electroacupuncture , Humans , Hyperlipidemias
8.
Article | IMSEAR | ID: sea-199677

ABSTRACT

Background: Computer assisted learning (CAL) in the classroom as well as laboratory in the medical profession has been rising in the present scenario worldwide. CAL can replace laboratory based animal experiments to a large extent and prevent the unnecessary harm or killing of animals. In India, only few studies have been conducted on CAL till date. Aims and objective of the study was to evaluate the knowledge and perception on CAL among undergraduate medical students.Methods: This cross sectional study was carried out on the medical students (MBBS-Second Professional) in the department of pharmacology at Guru Gobind Singh Medical College and Hospital, Faridkot, Punjab, India. Feedback was obtained from 105 students who had already performed animal experiments in CAL laboratory.Results: It was seen that Computer simulated models (CSMs) are useful for undergraduate teaching (by 98.1%), CAL enrich learning experience about drug effects (by 97.1%), CSMs in CAL is user friendly (by 97.1%) and use of CSMs in teaching is ethical (by 97.1%).Conclusions: CAL is an innovative teaching and learning technique for the medical students. This is an effective mode of teaching pharmacology to the students and it also helps the students to apply their theoretical knowledge of drugs to the practical aspects (without use of animal) on computer simulated models.

9.
Journal of Practical Radiology ; (12): 783-785, 2018.
Article in Chinese | WPRIM | ID: wpr-696911

ABSTRACT

Objective To evaluate the change of serum creatinine (Scr) before and after administration of contrast agent in different dose,to observe the difference of dog's kidney tissue with electron microscopy and investigate the effect of contrast agent on renal function.Methods Twelve dogs were divided into four groups randomly:the control group,the low dose group,the moderate dose group and high dose group.After the administration of different doses of iodine contrast agent at the same rate,the changes of Scr and microscopic structure were compared before administration and 48 hours later.Results The differences of Scr before and 48 hours after administration were (4.6±1.6) μmol/L,(6.7±2.5) μmol/L,(6.9±4.5) μmol/L,(5.1± 1.9) μmol/L for control group,low dose group,moderate dose group and high dose group,respectively.There was no statistically significant difference among the groups (P >0.05).In high dose group,the mitochondria of tubular epithelial cells were swelling and obvious vacuoles were observed.Only a small amount of vacuoles existed in the renal tubular epithelial cells in low dose group.Conclusion Compared with the moderate and high dose group,the low-dose iodine contrast agent have less damage to the kidney cells of the dogs.

10.
Article in English | WPRIM | ID: wpr-713484

ABSTRACT

The aim of this study is to evaluate the reporting quality of animal experiments in Korea using the Animals in Research: Reporting In Vivo Experiments (ARRIVE) guideline developed in 2010 to overcome the reproducibility problem and to encourage compliance with replacement, refinement and reduction of animals in research (3R's principle). We reviewed 50 papers published by a Korean research group from 2013 to 2016 and scored the conformity with the 20-items ARRIVE guideline. The median conformity score was 39.50%. For more precise evaluation, the 20 items were subdivided into 57 sub-items. Among the sub-items, status of experimental animals, housing and husbandry were described under the average level. Microenvironment sub-items, such as enrichment, bedding material, cage type, number of companions, scored under 10%. Although statistical methods used for the studies were given in most publications (84%), sample size calculation and statistical assumption were rarely described. Most publications mentioned the IACUC approval, but only 8% mentioned welfare-related assessments and interventions, and only 4% mentioned any implications of experimental methods or findings for 3R. We may recommend the revision of the present IACUC proposal to collect more detailed information and improving educational program for animal researchers according to the ARRIVE guideline.


Subject(s)
Animal Care Committees , Animal Experimentation , Animals , Compliance , Ethics , Friends , Housing , Humans , Korea , Sample Size
11.
Article in Chinese | WPRIM | ID: wpr-619765

ABSTRACT

Animal experiment on influenza virus infection carries certain biohazard risk, with a threat to the health of researchers and public health.The risk levels differ by influenza virus types and subtypes.This article combs the domestic and national laws and rules, and explores the biosafety management of animal study on influenza virus.

12.
Article in Chinese | WPRIM | ID: wpr-668694

ABSTRACT

The precision medical research in China is moving towards a new vigorous development stage. As an important contributor to clinical research, laboratory animals are becoming the key support and important guarantee to promote the development of precision medical diagnosis and treatment. At present, the evaluation system for animal experiments in China is not perfect enough,especially in the field of precision medicine. Under the background of domestic research and policy drive,we should learn from foreign experience and grasp the opportunities to promote and improve the evaluation system suitable for animal experiments in China.

13.
Article in Chinese | WPRIM | ID: wpr-668618

ABSTRACT

Objective To evaluate the clinical effectiveness of venipuncture using the fluorescent intravenous indwelling catheter in dark environment in dog experiments. Methods Six dogs were randomly divided into three groups of two dogs each, and each dog was subjected to 40 times of venipuncture performed on a foreleg with normal intravenous indwelling catheter and fluorescent intravenous indwelling catheter, respectively, under high(101 -105 lux), moderate (10.2-100 lux)or low(5 -10 lux)brightness conditions. The success rates and time consumption of those two procedures were analyzed and compared statistically. Results The success rate and time consumption for venipuncture using fluorescent and normal intravenous indwelling catheter under low brightness were 82.5% and(204.36 ± 13.13)s vs. 40.0% and(249.35 ± 17.98)s, those performed under moderate(simulating morning and dawn)brightness were 90.0% and(194.86 ± 8.60)s vs. 67.5% and(206.37 ± 9.70)s, all showed a significantly higher efficiency of the venipuncture using fluorescent intravenous indwelling catheter(P < 0.05). Conclusions The use of fluorescent intravenous indwelling catheter has the advantages of higher success rate and shorter time consumption for venipuncture under low and moderate brightness conditions.

14.
Braz. dent. j ; 27(3): 278-283, May-June 2016. tab, graf
Article in English | LILACS | ID: lil-782828

ABSTRACT

Abstract The aim of this study was to evaluate histomorphometrically the influence of two techniques of dental implant site preparation on bone healing around titanium implants. Fifteen male Wistar rats (±300 g) were used in the study. Each tibia was randomly assigned to receive the implant site preparation either with a conventional drilling technique (control - DRILL group) or with a piezoelectric device (PIEZO group). The animals were sacrificed after 30 days and then the following histomorphometric parameters were evaluated (percentage) separately for cortical and cancellous regions: proportion of mineralized tissue (PMT) adjacent to implant threads (500 μm adjacent); bone area within the threads (BA) and bone-implant contact (BIC). The results demonstrated that there were no statistically significant differences between both groups for cancellous BIC (p>0.05) and cortical PMT (p>0.05). On the other hand, a higher percentage of BA was observed in the PIEZO group in the cortical (71.50±6.91 and 78.28±4.38 for DRILL and PIEZO groups, respectively; p<0.05) and cancellous regions (9.62±4.06 and 19.94±14.18 for DRILL and PIEZO groups, respectively; p<0.05). The piezosurgery also showed higher PMT values in the cancellous zone (9.35±5.54 and 18.72±13.21 for DRILL and PIEZO groups, respectively; p<0.05). However, the DRILL group presented better results for BIC in cortical region (80.42±10.88 and 70.25±16.93 for DRILL and PIEZO groups, respectively; p<0.05). In conclusion, for the implant site preparation, the piezosurgery was beneficial to bone healing rates in the cancellous bone region, while the drill technique produced better results in the cortical bone.


Resumo O objetivo deste estudo foi avaliar histomorfometricamente a influência de duas técnicas de preparo para implante dentário sobre a reparação óssea ao redor de implantes de titânio. Foram utilizados 15 ratos machos Wistar, com aproximadamente 300 g. Uma tíbia dos animais foi aleatoriamente selecionada para o preparo do leito para instalação de um implante de titânio com um motor rotatório convencional (Grupo ROTATÓRIO) e a outra com ultrassom cirúrgico piezoelétrico (Grupo PIEZO). Após 30 dias, os animais foram sacrificados e foram avaliados os seguintes parâmetros histomorfométricos (em porcentagem), separadamente, para a região cortical e medular: a proporção de tecido mineralizado (PTM) na região adjacente ao implante (500 μm adjacentes); área de tecido mineralizado (AO) dentro dos limites das roscas do implante e a extensão de tecido ósseo em contato direto (CD) com a superfície do implante. Os resultados deste estudo mostraram que não foram observadas diferenças para CD na região medular (p>0,05) e para PTM na região cortical (p>0,05). Por outro lado, um maior preenchimento das roscas foi observado quando utilizou-se ultrassom cirúrgico piezoelétrico tanto na região cortical (71,50±6,91 e 78,28±4,38 para os grupos ROTATÓRIO e PIEZO, respectivamente; p<0,05) quanto na região medular (9,62±4,06 e 19,94±14,18 para os grupos ROTATÓRIO e PIEZO, respectivamente; p<0,05). Resultados semelhantes foram observados para o parâmetro PTM na região medular (9,35±5,54 e 18,72±13,21 para os grupos ROTATÓRIO e PIEZO, respectivamente; p<0,05). No entanto, o grupo ROTATÓRIO foi superior ao grupo PIEZO em relação a CD na região cortical (80,42±10,88 e 70,25±16,93 para os grupos ROTATÓRIO e PIEZO, respectivamente; p<0,05). Pode-se concluir que, para o preparo do leito para implantes, a piezocirurgia favoreceu o reparo ósseo na região medular, enquanto a técnica convencional promoveu melhores resultados no osso cortical.


Subject(s)
Animals , Male , Rats , Dental Implants , Piezosurgery , Titanium , Rats, Wistar
15.
Article in English | IMSEAR | ID: sea-179417

ABSTRACT

To evaluate student opinion on use of CAL in the MBBS 2nd professional experimental practical pharmacology curriculum.Also to get their views on the advantages and disadvantages of using CAL over traditional methods. A cross-sectional questionnaire based study was conducted at HIMS, Dehradun and Uttarakhand. After explaining the purpose of the study, voluntary inform consent was taken from the subjects before the start of the study. Students were instructed on experiments followed by computer simulation of the same. Thereafter avalidated self-designed questionnaire form was duly filled by each respondent.Out of the total 98 students, 88 (90%) of these thought that CAL is an effective method of teaching practical aspects. They also agreed to the fact that CAL should be conducted as an adjuvant to practical classes. Majority of students agreed that using CAL was advantageous to them .CAL is accepted as a welcome change byundergraduate students. However on the other hand practical knowledge of how to do the experiment is lost.

16.
Article in Chinese | WPRIM | ID: wpr-508529

ABSTRACT

Cerebrolysin is an aqueous mixture of amino acids extracted from porcine brain.Cerebrolysin, contains a variety of amino acids and low molecular weight peptides, are permeable to the blood-brain barrier and blood-ocular barrier and can directly act on neurons in the CNS.As amino acid-based neuroprotective reagents, cerebrolysin can improve the neuronal metabolism, promote synapse formation and induce neuronal differentiation, and thus elicit neuroprotective efffectagainst insults such as ischemia , neurotoxins and so on.Cerebrolysin is clinicly used to treat eye diseases such as traumatic eye damage, glaucoma, optic atrophy ect.This paper systematically summarizes the studies on preclinical and clinical application of cerebrolysin in ophthalmology.

17.
Article in English | WPRIM | ID: wpr-202735

ABSTRACT

OBJECTIVES: The purpose of this study was to investigate the effects of low-level laser therapy (LLLT) with a diode gallium-aluminum-arsenide (Ga-Al-As) low-level laser device on the healing and attachment of titanium implants in bone. MATERIALS AND METHODS: Thirteen New Zealand white male rabbits weighing 3.0+/-0.5 kg were used for this study. Dental titanium implants (3.75 mm in diameter and 8.5 mm in length, US II RBM plus fixture; Osstem, Seoul, Korea) were implanted into both femurs of each rabbit. The rabbits were randomly divided into a LLLT group and a control group. The LLLT was initiated immediately after surgery and then repeated daily for 7 consecutive days in the LLLT group. Six weeks and 12 weeks after implantation, we evaluated and compared the osseointegration of the LLLT group and control group, using histomorphometric analysis, removal torque testing, and resonance frequency analysis (RFA). The results were statistically significant when the level of probability was 0.05 or less based on a non-parametric Mann-Whitney U-test. RESULTS: The implant survival rate was about 96%. Histologically and histomorphometrically, we observed that the titanium implants were more strongly attached in LLLT group than in control group. However, there was no significant difference between the LLLT group and control group in removal torque or RFA. CONCLUSION: Histologically, LLLT might promote cell-level osseointegration of titanium implants, but there was no statistically significant effects.


Subject(s)
Animal Experimentation , Bone Density , Dental Implants , Femur , Humans , Low-Level Light Therapy , Male , New Zealand , Osseointegration , Rabbits , Seoul , Survival Rate , Titanium , Torque
18.
Article in English | IMSEAR | ID: sea-165086

ABSTRACT

Recently, a lot have been written and discussed about animal experiments and ethics. Still there is too much confusion among academicians and researchers about the future of use of animals in biomedical research and up to what extent their use in laboratory, research institutions, and medical colleges. This article highlighted and discussed about various aspects of this burning issue along with several pros and cons.

19.
Chongqing Medicine ; (36): 4917-4921, 2015.
Article in Chinese | WPRIM | ID: wpr-484031

ABSTRACT

Objective To observe the feasibility and security of the embolization with medical adhesive on renal artery in rab‐bits by animal experiments ,and explore the effectiveness of different concentration of embolic agents on the process and the effect of embolization for vascular ,and provide guidance for clinical application of medical adhesive .Methods A mixture of different ratios of lipiodol and medical adhesive were used to embolize the renal artery in 18 rabbits ,the usage and effects of embolization were ob‐served .Results Embolic agents were seen under the fluoroscopy ,easy to control and inject transcatheter ,also repeated injected by one micro‐catheter safety and effectively .Angiography review showed a reliable embolization ,no vascular recanalization and no spill‐over of contrast agent .Optical microscope ,after HE staining in postoperative pathological examination ,showed irregular shaped embolic agents or branch like refractive index of transparent material in the embolized artery .In 5∶1 group and 4∶1 group ,arteri‐ole and small artery were mainly embolized ,there was no significant difference between the two groups(P>0 .05);Whereas medium sized artery was mainly embolized in 3∶1 group ,compared with the 5∶1 group and 4∶1 group ,the differences was statistically significant(P<0 .05) .After operation ,renal function was transient damage ,leukocyte was transient increasing ,one week later ,both of renal function and leukocyte were recovered ,and there was no significantly renal function anomaly .Conclusion It is feasible ,safe and effective for medical adhesive to embolize rabbits renal artery ,the different concentration of the embolic agents can embolize the different branches of rabbit renal artery .

20.
Article in English | WPRIM | ID: wpr-217179

ABSTRACT

PURPOSE: The preferred material for bone augmentation beyond the envelope of skeletal bone is the bone block graft, due to its dimensional stability. We evaluated the necessity of rigid fixation for the bone block graft, and compared the bone regeneration and volume maintenance associated with grafting using a synthetic hydroxyapatite block (HAB) and an autogenous bone block (ABB) without rigid fixation on rabbit calvaria over two different periods. METHODS: Cylinder-shaped synthetic HAB and ABB were positioned without fixation on the rabbit calvarium (n=16). The animals were sacrificed at 4 or 8 weeks postoperatively, and the grafted materials were analyzed at each healing period using microcomputed tomography and histologic evaluation. RESULTS: Integration of the graft and the recipient bed was observed in all specimens, although minor dislocation of the graft materials from the original position was evident in some specimens (six ABB and ten HAB samples). A tendency toward progressive bone resorption was observed in the grafted ABB but not in the grafted HAB, which maintained an intact appearance. In the HAB group, the area of new bone increased between 4 and 8 weeks postoperatively, but the difference was not statistically significant. CONCLUSIONS: The nonfixed HAB was successfully integrated into the recipient bed after both healing periods in the rabbit calvaria. In spite of limited bone formation activity in comparison to ABB, HAB may be a favorable substitute osteoconductive bone material.


Subject(s)
Animal Experimentation , Animals , Bone Regeneration , Bone Resorption , Joint Dislocations , Durapatite , Hydroxyapatites , Osteogenesis , Skull , Transplants , X-Ray Microtomography
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