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1.
Braz. j. biol ; 84: e254234, 2024. tab, graf
Article in English | LILACS-Express | MEDLINE, LILACSEXPRESS, LILACS, VETINDEX | ID: biblio-1364499

ABSTRACT

Abstract Due to the severe side effects revealed by most of the currently used antidiabetic medicines, search for finding new and safe drugs to manage diabetes is continued. Naphthoquinones possessing strong antioxidant properties have been employed as candidates for diabetes therapy. Present study is aimed at finding the antioxidant and hypoglycaemic potential of some novel derivatives of 2-phenylamino-1,4-naphthoquinones (PAN) including chloro, nitro, methyl and bromo (5a-d) derivatives synthesized by single pot experiment. Product crystals were purified by TLC and characterized by FT-IR. The antioxidant potential of the compounds was assayed through DPPH radical scavenging and reducing power activities noted as UV-vis. absorbance. The DPPH assay has showed the powerful antioxidant activity of nitro and bromo derivatives, while the nitro derivative showed the significant reduction potential towards FRAP assay. Hypoglycaemic potential of the compounds was studied in rat animal model. All synthesized compounds revealed better hypoglycaemic activity; however, the chloro-derivative exhibited the more potent hypoglycaemic activity showing about 43% reduction in the mean blood glucose levels of the treated animals. As the bioreduction of naphthoquinones may be influenced by changing its redox properties, it has been noticed that the e-donating resonance effect (+R) of 'chloro' group has shown the significant effects on biological activity through stabalization of its imine form which limits the potential of generation of free radicals during bioreduction of quinones and thus has been proposed as the reason of its hypoglycaemic activity. Future studies employing the properties of e-donating groups of PAN may optimize the drug-receptor interaction for better drug designing and drug development strategies against diabetes and also for the clinical trials.


Resumo Em razão dos graves efeitos colaterais causados pela maioria dos medicamentos antidiabéticos atualmente utilizados, continua a busca por novos medicamentos seguros para o controle do diabetes. As naftoquinonas, que possuem fortes propriedades antioxidantes, têm sido empregadas como candidatas à terapia do diabetes. O presente estudo visa encontrar o potencial antioxidante e hipoglicemiante de alguns novos derivados de 2-fenilamino-1,4-naftoquinonas (PAN), incluindo derivados de cloro, nitro, metil e bromo (5a-d) sintetizados por experimento em pote único. Os cristais do produto foram purificados por TLC e caracterizados por FT-IR. O potencial antioxidante dos compostos foi testado por meio de atividades de sequestro de radicais DPPH e redução de energia observada como absorção no UV-vis. O ensaio DPPH mostrou a poderosa atividade antioxidante dos derivados nitro e bromo, enquanto o derivado nitro mostrou o potencial de redução significativo para o ensaio FRAP. O potencial hipoglicêmico dos compostos foi estudado em modelo animal de rato. Todos os compostos sintetizados revelaram melhor atividade hipoglicemiante; no entanto, o derivado cloro apresentou atividade hipoglicêmica mais potente, com redução de 43% nos níveis médios de glicose no sangue dos animais tratados. Como a biorredução de naftoquinonas pode ser influenciada pela alteração de suas propriedades redox, notou-se que o efeito da doação eletrônica por ressonância (+R) do grupo "cloro" tem sido significativo na atividade biológica por meio da estabilização de sua forma imina, que limita o potencial de geração de radicais livres durante a biorredução de quinonas, e, portanto, tem sido proposto como a razão de sua atividade hipoglicemiante. Estudos futuros empregando as propriedades de grupos de doação eletrônica de PAN podem otimizar a interação droga-receptor para melhor planejamento de medicamentos e estratégias de desenvolvimento de medicamentos contra o diabetes e também para os ensaios clínicos.

2.
Braz. j. biol ; 83: e245329, 2023. graf
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1285618

ABSTRACT

Abstract The cold storage of milt implies potentials alterations in its quality because the storage generates as main process, free radicals that produce spermatozoa membrane lipids damage with the consequent motility and fertilising capacity disruptions. To decrease the damage generated by free radicals the cells have antioxidant defences (proteins, enzymes, and low molecular weight substances). The objective of the present study evaluated the time storage effect and different antioxidants prepared in spermatic diluents on sperm viability of O. mykiss milt stored at 4°C. The two-way ANOVA denoted that the time storage and antioxidant influence have significant effects separated or combined on viability parameters (sperm motility and viability, proteins concentrations and superoxide dismutase enzymatic activity in seminal plasma). In contrast, only the storage time affected the fertilising capacity and catalase enzymatic activity in seminal plasma. The resulting analysis can conclude that the antioxidant presence improves the viability of cold stored milt, especially the transport conditions and the antioxidants allow the fecundity despite motility decrease.


Resumo O armazenamento a frio de leite implica potenciais alterações em sua qualidade, pois gera como processo principal radicais livres que provocam danos aos lipídios da membrana dos espermatozoides, com as consequentes alterações na motilidade e na capacidade de fertilização. Para diminuir os danos causados pelos radicais livres, as células têm defesas antioxidantes (proteínas, enzimas e substâncias de baixo peso molecular). O presente estudo avaliou o efeito do tempo de armazenamento e diferentes antioxidantes preparados em diluentes espermáticos no armazenamento de viabilidade de O. mykiss milt a 4°C. A ANOVA de duas vias denotou que o armazenamento no tempo e a influência antioxidante têm efeitos significativos separados ou combinados nos parâmetros de viabilidade (motilidade espermática, viabilidade espermática, concentrações de proteínas e atividade enzimática da superóxido dismutase no plasma seminal), enquanto apenas o tempo de armazenamento afetou a capacidade de fertilização e atividade enzimática da catalase no plasma seminal. A análise resultante pode concluir que a presença de antioxidante melhora a viabilidade do leite frio, especialmente as condições de transporte, e os antioxidantes permitem a fecundidade apesar da diminuição da motilidade.


Subject(s)
Animals , Male , Semen Preservation/veterinary , Oncorhynchus mykiss , Sperm Motility , Spermatozoa , Cryopreservation , Antioxidants
3.
Arch. argent. pediatr ; 120(3): 174-179, junio 2022. tab
Article in English, Spanish | LILACS, BINACIS | ID: biblio-1368143

ABSTRACT

Introducción. La exposición ambiental a plomo (Pb) aún constituye un problema de salud pública, particularmente para los niños. El estrés oxidativo podría representar un mecanismo primario asociado a su toxicidad. El objetivo del presente estudio fue determinar los niveles de Pb en sangre (Pb-S) en niños de 1 a 6 años de La Plata y alrededores con exposición ambiental, y su relación con biomarcadores de estrés oxidativo. Población y métodos. Estudio analítico de corte transversal. Se evaluaron niños clínicamente sanos de 1 a 6 años. Se determinaron los niveles de Pb-S, las actividades de enzimas antioxidantes y el grado de peroxidación lipídica. Se utilizó el paquete estadístico R versión 3.5.1. Resultados. Participaron 131 niños, mediana de edad 2,33 años. La media geométrica de los niveles de Pb-S fue 1,90 µg/dL; el 32 % presentó plombemias cuantificables y el 3 %, niveles ≥5 µg/dL (referencia internacional). Al comparar los biomarcadores de estrés oxidativo según los niveles de Pb-S, solo se observó diferencia significativa entre las medianas de las sustancias reactivas al ácido tiobarbitúrico (TBARS): 12,0 versus 10,0 nmol MDA/mL plasma; p = 0,02. Asimismo, la correlación entre las plombemias y las TBARS fue positiva (r = 0,24; p = 0,012). Conclusiones. La mayoría de los niños mostraron niveles de Pb-S menores a los límites recomendados por agencias internacionales, que si bien, no producen alteraciones en la actividad de enzimas antioxidantes, sí inducen peroxidación lipídica. Estos resultados reflejan la utilidad de este biomarcador como una herramienta diagnóstica temprana para evaluar los efectos subtóxicos del Pb.


Introduction. Environmental exposure to lead is still a major public health problem, especially in children. Oxidative stress may be a primary mechanism associated with toxicity. Theobjective of this study was to measure blood lead levels (BLLs) in children aged 1 to 6 years expos to lead in La Plata and suburban areas and their relation to oxidative stress biomarkers. Population and methods. Cross-sectional,analytical study. Clinically healthy children aged1 to 6 years were analyzed. BLLs, antioxidant enzyme activity, and extent of lipid peroxidation were measured. The statistical softwarepackage R, version 3.5.1, was used. Results. A total of 131 children participated; their median age was 2.33 years. The geometric mean of BLLs was 1.90 µg/dL; 32% showed a measurable BLL and 3%, BLLs ≥ 5 µg/dL (international reference). The comparison ofoxidative stress biomarkers based on BLshowed a significant difference in median thiobarbituric acid reactive substances (TBARS):12.0 versus 10.0 nmol MDA/mL of plasma;p = 0.02. In addition, the correlation between BLLs and TBARS was positive (r = 0.24; p = 0.012 Conclusions. Most children had a BLL below the limit recommended by international agencies; although such BLLs do not affantioxidant enzyme activity, they can induce lipid peroxidation. These results demonstrate theusefulness of this biomarker as an early diagnosistool to assess subtoxic lead effects.


Subject(s)
Humans , Infant , Child, Preschool , Child , Lead/analysis , Lead Poisoning/diagnosis , Argentina , Biomarkers , Cross-Sectional Studies , Thiobarbituric Acid Reactive Substances , Oxidative Stress , Environmental Exposure/adverse effects , Antioxidants
4.
Journal of Clinical Hepatology ; (12): 392-396, 2022.
Article in Chinese | WPRIM | ID: wpr-920890

ABSTRACT

Objective To investigate the effect of the mitochondria-targeted antioxidant SS-31 on acute liver injury in a mouse model of sepsis. Methods A total of 24 adult male C57BL/6J mice were randomly divided into control group, control+SS-31 group, lipopolysaccharide (LPS) group, and LPS+SS-31 group, with 6 mice in each group. The mice were given intraperitoneal injection of LPS (10 mg/kg) to establish a model of sepsis and acute liver injury, followed by intraperitoneal injection of SS-31 (10 mg/kg) for treatment, and the mice in the control group were given intraperitoneal injection of an equal volume of PBS solution, followed by intraperitoneal injection of an equal volume of normal saline. After 12 hours, ELISA was used to measure the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), reactive oxygen species (ROS), superoxide dismutase (SOD), tumor necrosis factor-α (TNFα), interleukin-1β(IL-1β), and interleukin-6 (IL-6), and HE staining was used to observe liver histopathological changes. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t -test was used for further comparison between two groups. Results Compared with the LPS group, the LPS+SS-31 group had significant reductions in the serum levels of ALT (140.05±12.22 U/L vs 102.64±8.75 U/L, P < 0.05) and AST (80.22±4.71 U/L vs 69.26±5.37 U/L, P < 0.05) and the levels of ROS (1 030.21±115.87 pg/mL vs 847.84±63.65 pg/mL, P < 0.05), TNFα (767.18±60.60 ng/mL vs 698.89±16.99 ng/mL, P < 0.05), IL-1β (29.97±1.37 ng/mL vs 26.70±3.09 ng/mL, P < 0.05), and IL-6 (59.13±7.09 pg/mL vs 49.29±3.41 pg/mL, P < 0.05) in liver tissue. Compared with the control group based on HE staining, the LPS group showed destruction of hepatic lobular structure, inflammatory cell infiltration, ambiguous intercellular space, and hepatocyte swelling, while the LPS+SS-31 group showed alleviation of inflammatory cell infiltration and hepatocyte swelling. Conclusion The mitochondria-targeted antioxidant SS-31 can reduce the production of ROS, downregulate the highly expressed inflammatory factors in sepsis, and alleviate sepsis-related acute liver injury in mice.

5.
CoDAS ; 34(4): e20210076, 2022. tab, graf
Article in Portuguese | LILACS-Express | LILACS | ID: biblio-1356171

ABSTRACT

RESUMO Objetivo Investigar os efeitos da suplementação antioxidante com extrato de açaí no incômodo com o zumbido crônico e a relação com os níveis de ansiedade e metabolismo oxidativo, não excluindo a sobreposição de enfermidades. Método Ensaio clínico, randomizado, controlado por placebo. Participaram 30 indivíduos, com média de 50,5 anos, 14 do sexo masculino e 16 do feminino, com limiares auditivos normais ou perda auditiva sensorioneural até grau leve bilateralmente, divididos em dois grupos: Grupo Placebo (sem ativo) e Grupo Açaí (100mg de extrato de açaí). Aplicaram-se os seguintes procedimentos antes e após três meses dos tratamentos: Tinnitus Handicap Inventory (THI), Inventário de Ansiedade de Beck (BAI) e amostras de sangue para avaliação de biomarcadores de estresse oxidativo (Peroxidação Lipídica e Carbonilação de proteínas). Resultados Houve redução do incômodo do zumbido para o grupo açaí, verificado por meio do THI (p=0,006). Diferenças significativas foram constatadas na pontuação dos sintomas comuns para os quadros de ansiedade no grupo placebo (p=0,016) porém, o mesmo não foi observado para os biomarcadores de metabolismo oxidativo, apesar de haver uma diminuição dos valores pós-tratamento para os grupos. Conclusão A suplementação antioxidante oral, com extrato de açaí, manifestou efeitos favoráveis no zumbido, reduzindo o desconforto com o sintoma, independente da etiologia de base, podendo ser considerada uma modalidade de tratamento. Entretanto, o efeito dessa suplementação nos sintomas de ansiedade e em biomarcadores de estresse oxidativo precisa de maior investigação.


ABSTRACT Purpose To investigate the effects of antioxidant supplementation with açaí extract on the discomfort with chronic tinnitus and the relationship with the levels of anxiety and oxidative metabolism, not excluding the overlap of diseases. Methods Randomized, placebo-controlled clinical trial. 30 individuals participated, with an average of 50.5 years, 14 males and 16 females, with normal hearing thresholds or sensorineural hearing loss up to mild degree, divided into two groups: Placebo Group (without active) and, Açaí Group (100mg of açaí extract). The following procedures were applied before and after three months of treatments: Tinnitus Handicap Inventory (THI), Beck's Anxiety Inventory (BAI) and blood samples for evaluation of oxidative stress biomarkers (Lipid Peroxidation and Protein Carbonylation). Results There was a reduction in the discomfort of tinnitus for the açaí group verified through THI (p = 0.006). Significant differences were found in the score of common symptoms for anxiety disorders in the placebo group (p = 0.016), however, the same was not observed for oxidative metabolism biomarkers, although there was a decrease in post-treatment values for all groups. Conclusion Oral antioxidant supplementation, with açaí extract, showed favorable effects on tinnitus, reducing discomfort with the symptom, regardless of the underlying etiology, and can be considered a treatment modality. However, the effect of this supplementation on anxiety symptoms and oxidative stress biomarkers needs further investigation.

6.
Braz. oral res. (Online) ; 36: e034, 2022. tab, graf
Article in English | LILACS-Express | LILACS, BBO | ID: biblio-1364593

ABSTRACT

Abstract: This study aimed to analyze oxidative stress and the activity of antioxidant enzymes in the salivary glands of streptozotocin (STZ)-induced diabetic rats with ad libitum consumption of chamomile tea in substitution of water for 21 days. Rats were divided in two control groups (untreated control and treated control) and two diabetic groups (untreated diabetic and treated diabetic). Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activities, total antioxidant status (TAS), and malondialdehyde (MDA) concentrations were determined. The chemical composition of the chamomile essential oil revealed 39 compounds, accounting for 93.5% of the total oils. The polyphenolic profile of the tea showed the presence of apigenin, luteolin, umbelliferone, and esculetin. SOD, GPx, CAT, and TAS levels were lower in the parotid (PA) diabetic glands, but treatment increased their concentration in both the submandibular (SM) and PA diabetic salivary glands. Increased MDA levels were observed in the PA diabetic glands, which were decreased by the consumption of chamomile tea with a reduction in hyperglycemia compared to that in untreated diabetic rats. However, the SM diabetic glands showed no difference in the MDA content. The consumption of chamomile tea prevented oxidative stress in the PA glands of diabetic rats, exhibiting hypoglycemic and antioxidant effects. Thus, chamomile tea could be a potential candidate for preventing oral complications in diabetes mellitus.

7.
Arq. bras. cardiol ; 118(2): 435-445, 2022. tab, graf
Article in English, Portuguese | LILACS | ID: biblio-1364333

ABSTRACT

Resumo Fundamento O pterostilbeno (PS), um composto polifenólico natural e antioxidante, surge como uma intervenção promissora para minimizar danos do infarto agudo do miocárdio (IAM). Objetivo Este estudo teve como objetivo avaliar o desempenho do PS na promoção da homeostase redox nos pulmões e no ventrículo direito (VD) de animais infartados. Métodos Ratos Wistar machos (60 dias de idade) foram randomizados em três grupos: SHAM, IAM (infarto) e IAM+PS (IAM + pterostilbeno). Sete dias após o procedimento de IAM, os ratos foram tratados com PS (100 mg/kg/dia) por gavagem por oito dias. Os animais foram depois sacrificados e os pulmões e VD foram coletados para análise do balanço redox (diferenças foram consideradas significativas quando p<0,05). Resultados Nossos resultados mostram que o IAM desencadeia a interrupção redox no VD e nos pulmões, o que pode contribuir para danos induzido pelo IAM nesses órgãos. Consistentemente, o PS mitigou o estresse oxidativo e restaurou as defesas antioxidantes (Glutationa - GSH nos pulmões: SHAM = 0,79 ± 0,07; IAM = 0,67 ± 0,05; IAM + PS = 0,86 ± 0,14; p<0,05), indicando seu papel protetor neste cenário. Conclusão Nosso trabalho evidencia o potencial do uso de PS como abordagem terapêutica adjuvante após IAM para proteção dos tecidos pulmonares e cardíacos direitos.


Abstract Background Pterostilbene (PS), a natural and antioxidant polyphenolic compound emerges as a promising intervention in improving the myocardial infarction (MI) damages. Objetives This study aimed to evaluate PS actions in promoting redox homeostasis in lungs and right ventricle (RV) of infarcted animals. Methods Male Wistar rats (60 day-old) were randomized into three groups: SHAM, MI (infarcted), and MI+PS (MI+pterostilbene). Seven days after MI procedure, rats were treated with PS (100 mg/kg/day) via gavage for eight days. Animals were euthanized and the lungs and RV were harvested for analyses of redox balance (Differences were considered significant when p<0.05). Results Our results show that MI triggers a redox disruption scenario in RV and lungs, which can contribute to MI-induced damage on these organs. Consistently, PS mitigated oxidative stress and restored antioxidant defenses (GSH in lungs: SHAM= 0.79±0.07; MI=0.67±0.05; MI+PS=0.86±0.14; p<0.05), indicating its protective role in this scenario. Conclusions Our work evidences the PS potential use as an adjuvant therapeutic approach after MI focusing on protecting pulmonary and right-sided heart tissues.


Subject(s)
Animals , Male , Rats , Stilbenes/pharmacology , Oxidative Stress/drug effects , Heart Ventricles/drug effects , Lung/drug effects , Myocardial Infarction/complications , Myocardial Infarction/drug therapy , Rats, Wistar
8.
Acta biol. colomb ; 26(3): 414-422, sep.-dic. 2021. tab, graf
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1360036

ABSTRACT

RESUMEN Solanum ferruginium es una planta que crece en lugares perturbados como maleza, es de uso medicinal poco utilizada que presenta un gran potencial como fuente de antioxidantes debido a su alto contenido en polifenoles. Debido a esto se analizó el contenido de polifenoles, flavonoides, proantocianidinas y capacidad antioxidante in vitro (ensayo de captación de radicales DPPH) en hojas, tallos y planta completa de tres localidades (Las agujas, Parque el Nabo y Bosque la Primavera) de Zapopan, Jalisco, así como su toxicidad subcronica en hojas. En general se observó diferencia significativa (p≤ 0,05), en las muestras de las tres localidades, las hojas presentaron el mayor contenido de polifenoles (15,3±0,7 a 22±0,4 mg expresado como equivalente de ácido gálico (EAG/g) en muestras de Parque el Nabo, flavonoides (7,8±0,3 a 13,3±0,3 mg EC/g) y proantocianidinas (3,4±0,1 a 4,2±0,05 mg expresado como equivalente de catequina (EC/g) en el Bosque la Primavera. La capacidad antioxidante fue similar en todas las muestras, con valores de 8,3 a 17 μg/mL de concentración media inhibitora (CI50). En la prueba toxicológica, los ratones no mostraron signos de toxicidad a ninguna dosis por efecto de la administración de la planta en estudio, por lo que la dosis letal media (DL50) es > 15 000 mg/kg de peso corporal. El contenido de polifenoles y actividad antioxidante en S. ferruginium sobre todo en hojas indican un alto potencial con propiedades farmacológicas además de su inocuidad, por lo que es importante realizar estudios de sus compuestos fenólicos individuales, antes de ser utilizada en farmacología.


ABSTRACT Solanum ferrugineum is a plant that grows like a weed. It is a new medicinal plant with great potential as an antioxidant source due to its high polyphenol content. Because of this, polyphenol, flavonoid, proanthocyanidin, and antioxidant capacity in vitro (radical scavenging test by DPPH) were analyzed in leaves, stems, and whole plants from three localities (Las agujas, Nabo Park, and La Primavera Forest) as well as the subchronic toxicity evaluation in leaves. In general, there was a significant difference (p≤ 0.05) in all samples from the three localities. The leaves showed the highest polyphenol content (15.3±0.7 to 22±0.4 mg AGE/g) in samples from the Nabo Park, flavonoid (7.8±0.3 to 13.3±0,3 mg CE/g) and proanthocyanidins (3.4±0.1 to 4.2±0.05 mg CE/g) in La Primavera Forest. Antioxidant capacity was similar across all samples, showing values of 8.3 to 17 μg/mL of IC50. During the toxicology assay, animal specimens showed no signs of toxicity to the doses resulting from the administration of the plant under study so that LD50 > 15 000 mg/kg Bodyweight. The polyphenol content and antioxidant capacity obtained from S. ferruginium leaves, together with its safety, indicate a high pharmacological potential of this plant. Therefore, it is important to carry out studies of its phenolic compounds before being used in pharmacology.

9.
Acta toxicol. argent ; 29(2): 21-30, dic. 2021. graf
Article in Portuguese | LILACS-Express | LILACS | ID: biblio-1364282

ABSTRACT

Resumo Venenos sao uma substancia tóxica (composta por uma ou mais toxinas) que podem causando lesao fisiológica dependente da dose. As toxinas sao moléculas bioativas formadas principalmente por compostos enzimáticos e nao enzimático que porque provocam consequéncias indesejáveis nas presas, além disso, exibem atividades biológicas únicas, diversas e específicas que perturbam os processos fisiológicos normais. Entretanto, muitas toxinas, de diferentes animais, tém sido isoladas e muitas delas sao consideradas ótimas ferramentas para pesquisa básica e alvos terapéuticos. Foi relatado que o estresse oxidativo desempenha um papel fundamental na patogénese de várias doengas, como distúrbios neurodegenerativos, distúrbios cardiovasculares e cáncer. O mecanismo pelo qual as toxinas animais atuam nos parametros de estresse oxidativo em várias doengas, ainda nao está estabelecido. O foco principal desta revisao é destacar os principais estudos com toxinas animais como ferramenta terapéutica em diversas doengas, atuando no balango redox do organismo.


Abstract Venoms are a toxic substance (comprised of one or more toxins) that can cause dose-dependent physiological injury. Toxins are bioactive molecules formed primarily by enzymatic and non-enzymatic compounds that cause undesirable conse-quences in prey, in addition, exhibit unique, diverse and specific biological activities that disrupt normal physiological processes. However, many toxins, from different animals, have been isolated and many of them are considered great tools for basic research and therapeutic targets. Oxidative stress has been reported to play a key role in the pathogenesis of various diseases such as neurodegenerative disorders, cardiovascular disorders and cancer. How animal toxins act on oxidative stress parameters in several diseases is not yet established. The main focus of this review is to highlight the main studies with animal toxins as a therapeutic tool in several diseases, acting on the organism's redox balance.


Resumen Los venenos son sustancias tóxicas (compuestas por una o más toxinas) que pueden causar daño fisiológico dependiente de la dosis. Las toxinas son moléculas bioactivas formadas principalmente por compuestos enzimáticos y no enzimáticos que debido a que causan consecuencias indeseables en las presas, además, exhiben actividades biológicas únicas, diversas y específicas que alteran los procesos fisiológicos normales. Sin embargo, se han aislado muchas toxinas de diferentes animales, y muchos de ellos se consideran grandes herramientas para la investigación básica y dianas terapéuticas. Se ha informado que el estrés oxidativo juega un papel clave en la patogenia de diversas enfermedades, como los trastornos neurodegenerativos, enfermedades cardiovasculares y cáncer. El mecanismo por el cual las toxinas animales actúan sobre los parámetros de estrés oxidativo en vários enfermedades, aún no está establecido. El enfoque principal de esta revisión es resaltar los principales estudios con toxinas animales como herramienta terapéutica en diversas enfermedades, actuando en el equilibrio redox del organismo.

10.
NOVA publ. cient ; 19(37): 121-134, jul.-dic. 2021. tab
Article in English | LILACS-Express | LILACS | ID: biblio-1365195

ABSTRACT

Abstract Aim. In vitro antimicrobial activities of seven wines (5 reds and 2 whites) from the Douro region (Iberian Peninsule) against eleven clinical strains of Helicobacter pylori were evaluated. Methods. The disk diffusion method, using Columbia Agar supplemented with horse blood (CAB), were used to determine the antimicrobial properties of some wine components against H. pylori strains. Potential interactions of antioxidants contained in the wines and two antimicrobials (amoxicillin and metronidazole) were studied by the disk diffusion method. Results. All the tested strains showed growth in CAB supplemented with 9% of the tested wines but none of them grew in media supplemented with 45% and 67.5% of wine. Similarly, all the tested strains grew in media with the concentration of proanthocyanidins present in the different types of the studied wines. The Minimal Inhibitory Concentration (MIC) values of the wine antioxidant components tested (benzoic acid, catechin, quercetin, and resveratrol) indicate that resveratrol was the most powerful inhibitory substance against H. pylori. An effect of potentiation between amoxicillin and metronidazole and the antioxidants tested was also established. The interaction of amoxicillin and resveratrol or metronidazole and catechin increased the antimicrobial activity against H. pylori. Conclusions. The results obtained suggested a potential role of resveratrol as a chemopreventive agent for H. pylori infection.


Resumen Objetivo. Se evaluó las actividades antimicrobianas in vitro de siete vinos (5 tintos y 2 blancos) de la región del Duero (Peninsula Ibérica) frente a once cepas de Helicobacter pylori de origen clínico. Métodos. Para determinar las propiedades antimicrobianas de algunos componentes del vino sobre las cepas de H. pylori se utilizaron las técnicas de difusión en disco en placas de agar Columbia suplementado con sangre de caballo (CAB). La potential interacción entre las sustancias antioxidantes presentes en los vinos y dos antimicrobianos (amoxicilina y metronidazol) se determinó usando la técnica de difusión en disco. Resultados. Todas las cepas ensayadas mostraron crecimiento en CAB suplementado con el 9% de los vinos analizados, pero no se obtuvo crecimiento de ninguna de las cepas en medios suplementados con el 45% y el 67,5% de vino. Asimismo, todas las cepas ensayadas crecieron en medios con la concentración de proantocianidinas presentes en los diferentes tipos de vinos estudiados. Los valores de concentración mínima inhibitoria (CMI) de los componentes antioxidantes de los vinos ensayados (ácido benzoico, catequina, quercetina y resveratrol) indican que el resveratrol fue la sustancia más potente en la inhibición del crecimiento de H. pylori. También se estableció un efecto de potenciación entre amoxicilina y metronidazol y los antioxidantes ensayados. Las interacciones amoxicilina + resveratrol y metronidazol + catequina aumentaron la actividad antimicrobiana contra H. pylori. Conclusiones. Los resultados obtenidos sugieren un papel potencial del resveratrol como agente quimiopreventivo de la infección por H. pylori.

11.
Arch. endocrinol. metab. (Online) ; 65(6): 676-683, Nov.-Dec. 2021. tab, graf
Article in English | LILACS | ID: biblio-1349986

ABSTRACT

ABSTRACT Objectives: Paraoxonase 1 (PON1) is an enzyme that has antioxidant potential, which confers a protective effect against the atherosclerotic process. However, studies associating genetics, dietary patterns and PON1 activity in individuals with cardiovascular disease (CVD) are scarce. Thus, the aim of the current study was to evaluate the influence of dietary factors on serum PON1 in CVD patients. Subjects and methods: Cross-sectional, sub-study of the BALANCE Program Trial. All patients aged 45 years or older and had evidence of established atherosclerotic disease in the preceding 10 years. Body weight, height, waist circumference, blood pressure, lipid profile and fasting glucose were collected. Food intake was assessed with 24-h dietary recall. Data was analyzed using SAS University Edition and a P value ≤ 0.05 was considered statistically significant. Sample was divided into three groups, according to the PON1 T(-107)C genotype (CC, CT and TT) and serum PON1 activity (Low, Medium, High). Results: There were no genotype differences for major factors. However, the systolic blood pressure was lower for CT individuals (p<0.05). Intake of cholesterol, saturated fatty acids (SFA) and monounsaturated fatty acids (MUFAS) was higher in patients with lower PON1 activity. Lipid ingestion tended to be higher in patients with lower PON1 activity (p=0.08). In the multivariate logistic regression model, SFA intake (P=0.03), genotype (P=0.09), gender (P=0.04), age (P=0.07) and carbohydrate intake (P=0.16) contributed the most to the serum PON1 activity. Conclusion: Based on these findings, nutritional guidance for these patients becomes essential, since dietary components interact with serum PON1 activity more than genotype.


Subject(s)
Humans , Cardiovascular Diseases , Aryldialkylphosphatase/genetics , Cross-Sectional Studies , Fatty Acids , Genotype , Lipids
12.
Arq. bras. med. vet. zootec. (Online) ; 73(4): 799-811, Jul.-Aug. 2021. tab, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1285263

ABSTRACT

This study aimed to evaluate the ultrastructural morphometry of bovine embryos produced in vitro grown at different concentrations of antioxidants. After in vitro maturation and fertilization, the presumptive zygotes were assigned into five treatments. T1) without the addition of any antioxidants (negative control); T2) addition of 50µM/mL cysteamine; and T3, T4 and T5) adding 2.5µg/mL, 5.0µg/mL or 10.0µg/mL of the antioxidants derived from the oily extract from Lippia origanoides, respectively. On D7 of culture, the embryos in the blastocyst stage were fixed and prepared for electron transmission microscopy. These were evaluated for the proportion of cytoplasm-to-nucleus, cytoplasm-to-mitochondria, cytoplasm-to-vacuoles, cytoplasm-to-autophagic vacuoles and cytoplasm-to-lipid droplets. Blastocysts cultured in media containing oily extract of Lippia origanoides presented morphological characteristics such as high cell:mitochondria ratio and low cell:vacuoles and cell:autophagic vacuole ratio, possibly been morphological indicators of embryonic quality. Inner cell mass (ICM) from blastocysts cultured in media without any antioxidants had the highest cell:vacuole ratio. Similar results were found in the trophectoderm (TE) cells of blastocysts from treatment 2. Embryo culture media supplemented with antioxidants derived from Lippia origanoides oil produced embryos with a higher cytoplasmic proportion of organelles, such as mitochondria. Also, treatments without any antioxidants or with the addition of cysteamine presented cytoplasmic vacuolization, a characteristic related to production of poor-quality embryos.(AU)


Este estudo teve como objetivo avaliar a morfometria ultraestrutural de embriões bovinos produzidos in vitro e cultivados em diferentes concentrações de antioxidantes. Após a maturação e a fertilização in vitro, os possíveis zigotos foram divididos em cinco tratamentos: T1) sem adição de antioxidantes (controle negativo); T2) adição de 50µM/mL de cisteamina; e T3, T4 e T5) adição de 2,5µg/mL, 5,0µg/mL ou 10,0µg/mL dos antioxidantes derivados do extrato oleoso de Lippia origanoides, respectivamente. No D7 de cultivo, os embriões em estágio de blastocisto foram fixados e preparados para microscopia eletrônica de transmissão. Estes foram avaliados para a proporção entre citoplasma e núcleo, citoplasma e mitocôndria, citoplasma e vacúolos, citoplasma e vacúolos autofágicos e citoplasma e gotículas lipídicas. Blastocistos cultivados em meio contendo extrato oleoso de Lippia origanoides apresentaram características morfológicas como alta relação célula:mitocôndria e baixa relação célula:vacúolos e célula:vacúolo autofágico, possíveis indicadores morfológicos de qualidade embrionária. A massa celular interna (MCI) de blastocistos cultivados em meio sem quaisquer antioxidantes teve a maior razão célula:vacúolo. Resultados semelhantes foram encontrados nas células do trofectoderma (TE) de blastocistos do tratamento 2. Portanto, o meio de cultivo embrionário suplementado com antioxidantes derivados do óleo de Lippia origanoides produziu embriões com maior proporção citoplasmática de organelas, como mitocôndrias. Além disso, tratamentos sem antioxidantes ou com adição de cisteamina apresentaram vacuolização citoplasmática, característica relacionada à produção de embriões de baixa qualidade.(AU)


Subject(s)
Blastocyst , Cysteamine , Lippia , Embryo, Mammalian/ultrastructure , In Vitro Techniques/veterinary , Antioxidants
13.
Ces med. vet. zootec ; 16(2): 9-29, mayo-ago. 2021. tab, graf
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1364664

ABSTRACT

Resumen El huevo es un alimento que presenta alto valor nutritivo, aporta elevada proporción de proteínas, vitaminas y minerales, relativamente bajo de energía, lo que lo convierte en un alimento con alta densidad de nutrientes. El objetivo de este estudio fue determinar el efecto de la inclusión de materias primas autóctonas del sur de Chile que contienen compuestos bioactivos en la alimentación de las gallinas criollas sobre la composición en ácidos grasos y capacidad antioxidante de sus huevos. Para esto, se elaboraron 8 dietas experimentales y una dieta de grano comercial y una dieta control, las dietas experimentales fueron formuladas en base a tres niveles de inclusión de fuentes de omega 3 (harina de salmón, chorito y microlaga) y tres niveles de fuentes de antioxidantes (harina de mora, frutilla y pepa de uva). El ensayo experimental tuvo duración de ocho semanas, donde se alimentaron 360 gallinas, distribuidas en 9 explotaciones avícolas. Al final de este período, los huevos de los tratamientos fueron liofilizados y se determinaron los ácidos grasos por cromatografía de gases y capacidad antioxidante por el método ABTS y DPPH. Los huevos, donde las gallinas fueron alimentadas con las dietas 7 y 8, compuestas por harina de alga y de pepa de uva, fueron aquellas que presentaron un mayor contenido del Ácido docosahexaenoico (DHA), 3,43 y 3,46 / 100 g respectivamente. Por otro lado, los huevos puestos por gallinas que se alimentaron de la dieta 5, compuestas por harina de salmón, chorito, mora y frutilla, fueron los que presentaron una mayor capacidad antioxidante.


Abstract The egg is a food that has high nutritional value, provides a high proportion of proteins, vitamins and minerals, relatively low in energy, which makes it a food with high nutrient density. The objective of this study was to determine the effect of the inclusion of native raw materials from southern Chile that contain bioactive compounds in the feed of Creole hens on the fatty acid composition and antioxidant capacity of their eggs. For this, 8 experimental diets were developed and a commercial grain diet and a control diet, the experimental diets were formulated based on three levels of inclusion of omega 3 sources (salmon meal, mussel and microlala) and three levels of sources of antioxidants (blackberry flour, strawberry and grape seed). The experimental trial lasted eight weeks, where 360 hens were fed, distributed in 9 poultry farms. At the end of this period, the eggs of the treatments were lyophilized and the fatty acids were determined by gas chromatography and antioxidant capacity by the ABTS and DPPH method. The eggs, where the hens were fed diets 7 and 8, composed of algae and grape seed meal, were those that presented a higher content of docosahexaenoic acid (DHA), 3.43 and 3.46 / 100 g respectively. On the other hand, the eggs laid by hens that were fed diet 5, composed of salmon meal, mussel, blackberry and strawberry, were those that presented the highest antioxidant capacity.


Resumo Os ovos têm alto valor nutricional, com uma alta proporção de proteínas, vitaminas e minerais, e uma ingestão energética relativamente baixa, tornando-os um alimento com alta densidade nutricional. Este estudo tem por objetivo analisar o efeito da inclusão de matérias-primas nativas do sul do Chile que contêm compostos bioativos na ração de galinhas crioulas sobre a composição de ácidos graxos e a capacidade antioxidante de seus ovos. Para tanto, foram elaboradas 8 dietas experimentais e uma dieta comercial de grãos, além de uma dieta controle. As dietas experimentais foram formuladas com base em três níveis de inclusão de fontes ômega 3 (farinhas de salmão, de mexilhão e de microalgas) e três níveis de fontes antioxidantes (farinhas de amora, de morango e de semente de uva). O ensaio experimental durou oito semanas. Durante o experimento, 360 galinhas em 9 granjas foram alimentadas com as dietas. Ao final desse período, os ovos dos tratamentos foram liofilizados, os ácidos graxos mensurados por cromatografia gasosa e a capacidade antioxidante, pelos métodos ABTS e DPPH. Os ovos de galinhas alimentadas com as dietas 7 e 8, compostas de farelo de algas e grainha de uva, apresentaram maior teor de ácido docosaexaenoico (DHA), 3,43 e 3,46 / 100 g, respectivamente. Por sua vez, os ovos postos por galinhas alimentadas com a dieta 5, composta por farinha de salmão, mexilhão, amora e morango, apresentaram a maior capacidade antioxidante.

14.
Bol. latinoam. Caribe plantas med. aromát ; 20(4): 416-426, jul. 2021. tab, ilus
Article in English | LILACS | ID: biblio-1369485

ABSTRACT

Reactive oxygen species are implicated in multiple pathological conditions including erectile dysfunction. This study evaluated the in vitro and in vivo antioxidant potential of the methanolic extracts of Inula glomerata and Salacia kraussii. The plant materials were pulverized and extracted with methanol. The phytochemical analysis, ability of the crude extracts to scavenge free radicals (ABTS, DPPH, NO.) in vitroas well as the total phenolic and flavonoid contents was investigated. In vivo, antioxidant potentials of the crude extracts (50/250 mg/kg body weight) were determined in an erectile dysfunction rat model. The phytochemical analysis revealed that both plants contain flavonoids, tannins, terpenoids, and alkaloids. The crude extracts at varying degree of efficiency, scavenged ABTS and DPPH radicals. The crude extracts at low concentrations (50 mg/kg b.w) significantly (p<0.05) diminished the level of malondialdehyde, augmented catalase activities and elevated glutathione levels. However, SOD activities were significantly boosted in a dose-dependent manner by the crude extracts. Therefore, I. glomerataand S. kraussiipossess antioxidant properties, hence, can serve as a therapeutic modality in the treatment of oxidative stress-induced erectile dysfunction.


Las especies reactivas de oxígeno están implicadas en múltiples condiciones patológicas, incluyendo la disfunción eréctil. Este estudio evaluó el potencial antioxidante in vitro e in vivo de extractos metanólicos de Inula glomeratay Salacia kraussii. Los materiales vegetales fueron pulverizados y extraídos con metanol. A estos extractos crudos se les llevó a cabo el análisis fitoquímico junto con el contenido total de fenólicos y flavonoides, así como se les investigó la capacidad in vitro para atrapar radicales (ABTS, DPPH, NO.). Los potenciales antioxidantes in vivo de los extractos crudos (50/250 mg/kg de peso corporal) se determinaron en un modelo en ratas con disfunción eréctil. El análisis fitoquímico reveló que ambas plantas contuvieron flavonoides, taninos, terpenoides y alcaloides. Los extractos crudos con un grado variable de eficiencia, atraparon a los radicales ABTS y DPPH. Los extractos crudos a bajas concentraciones (50 mg/kg p.c) significativamente (p<0.05) disminuyeron el nivel de malondialdehído, aumentaron las actividades de catalasa y elevaron los niveles de glutatión. Sin embargo, las actividades de SOD por los extractos crudos fueron significativamente dosis-dependientes. Así, los extractos de I. glomeratay S. kraussii mostraron propiedades antioxidantes, y por lo tanto, podrían servir como una alternativa terapéutica en el tratamiento de disfunción eréctil inducida por estrés oxidativo.


Subject(s)
Animals , Rats , Plant Extracts/pharmacology , Plant Extracts/chemistry , Inula/chemistry , Salacia/chemistry , Antioxidants/pharmacology , Antioxidants/chemistry , Sulfonic Acids/metabolism , Flavonoids/analysis , Reactive Oxygen Species , Rats, Sprague-Dawley , Oxidative Stress/drug effects , Asteraceae/chemistry , Celastraceae/chemistry , Benzothiazoles/metabolism , Phenolic Compounds/analysis , Phytochemicals/analysis , Nitric Oxide/metabolism
15.
Rev. med. vet. (Bogota) ; (42): 51-57, ene.-jun. 2021. graf
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1365910

ABSTRACT

Resumen El selenio (Se) es un componente indispensable dentro de los sistemas antioxidantes del organismo animal, lo que permite su correcto mantenimiento y desarrollo. Por este motivo, su deficiencia se encuentra relacionada con una serie de trastornos oxidativos, que se traducen en una mala salud, reproducción y rendimiento productivo en general. El objetivo del presente estudio fue evaluar el balance metabólico nutricional de Se, a través de la medición de las concentraciones sanguíneas del mineral en un establecimiento rural ubicado en la zona central de Chile, en donde existen antecedentes de episodios de mortandad compatibles con su deficiencia. Para esto, se tomaron muestras sanguíneas de caprinos pertenecientes al establecimiento (n = 32), las cuales fueron analizadas mediante espectrofotometría de absorción atómica en el laboratorio de bioquímica de la Estación Experimental Agropecuaria del Instituto Nacional de Tecnología Agropecuaria, ubicado en Balcarce, Argentina. Se obtuvo un promedio de Se sanguíneo de 34,16 ± 14,81 ppb, en donde el 100 % de los animales analizados presentaron valores inadecuados para la especie. En el caso de los caprinos juveniles (n = 5), se obtuvo un promedio de 18,48 ± 8,51 ppb. En el ganado adulto (n = 27), los valores bordearon concentraciones de 8,12 y 63,35 ppb. Se concluye que existe una deficiencia de Se crítica en los animales muestreados pertenecientes al establecimiento ubicado en la zona central de Chile.


Abstract Selenium (Se) is a component indispensable for the antioxidant systems of animal bodies. It enables a correct maintenance and development. For this reason, a Se deficiency is associated with some oxidative disorders that result in bad health, bad reproduction and poor productive performance in general. This study aims to evaluate the Se nutritional metabolic balance by measuring the blood concentrations thereof in a farm located in Central Chile, with existing background of mortality events compatible with a Se deficiency. To do so, blood samples were taken from goats belonging to this farm (n = 32) that were subject to atomic absorption spectrophotometry at the biochemistry laboratory in the Agricultural Experimental Station, Instituto Nacional de Tecnología Agropecuaria, located in Balcarce, Argentina. Blood average Se was measured at 34.16 ± 14.81 ppb and 100% of the studied animals showed inappropriate values for this species. In the young goats (n = 5) the average value was 18.48 ± 8.51 ppb. In the adult goats (n = 27) the values were near 8.12 and 63.35 ppb. It is concluded that there is a critical Se deficiency in the sample taken from the farm located in Central Chile.

17.
Arq. bras. cardiol ; 116(6): 1127-1136, Jun. 2021. tab, graf
Article in English, Portuguese | LILACS | ID: biblio-1278317

ABSTRACT

Resumo Fundamento O suco de laranja (SL) é rico em polifenóis com propriedades anti-inflamatórias e antioxidantes. Após o infarto do miocárdio (IM), mudanças complexas ocorrem na estrutura e na função cardíacas, processo conhecido como remodelação cardíaca (RC). O estresse oxidativo e a inflamação podem modular esse processo. Nossa hipótese foi a de que o consumo de SL atenua a RC após o IM. Objetivos Avaliar a influência do SL sobre a RC após IM pela análise de variáveis funcionais, morfológicas, de estresse oxidativo, de inflação, e de metabolismo energético. Métodos Um total de 242 ratos machos pesando entre 200 e 250g foram submetidos a um procedimento cirúrgico (ligação da artéria coronária ou cirurgia simulada). Sete dia após a cirurgia, os animais sobreviventes foram divididos para um dos quatro grupos: 1) SM, animais sham que receberam água e maltodextrina (n= 20); 2) SSL, animais sham que receberam SL (n= 20); 3) IM, animais infartados que receberam água e maltodextrina (n= 40); e 4) ISL, animais infartados que receberam SL (n = 40). A análise estatística foi realizada pelo teste de ANOVA com dois fatores com o teste de Holm-Sidak. Os resultados foram apresentados em média ± desvio padrão, e o nível de significância adotado foi de 5%. Resultados Três meses depois, o IM levou à hipertrofia do ventrículo esquerdo (VE), com disfunção sistólica e diastólica, e aumento nos mediadores inflamatórios e de estresse oxidativo. Os animais que consumiram SL apresentaram menor atividade da glutationa peroxidase e maior expressão da heme-oxigenase-1 (HO-1). Conclusão O SL atenuou a RC, e a HO-1 pode exercer um importante papel nesse processo.


Abstract Background Orange juice (OJ) is rich in polyphenols with anti-inflammatory and antioxidant properties. After myocardial infarction (MI), complex changes occur in cardiac structure and function, which is known as cardiac remodeling (CR). Oxidative stress and inflammation can modulate this process. We hypothesized that the consumption of OJ attenuates the CR after MI. Objectives To evaluate the influence of OJ on CR after MI by analysis of functional, morphological, oxidative stress, inflammation, and energy metabolism variables. Methods A total of 242 male rats weighing 200-250 g were submitted to a surgical procedure (coronary artery ligation or simulated surgery). Seven days after surgery, survivors were assigned to one of the four groups 1) SM, sham animals with water and maltodextrin (n= 20); 2) SOJ, sham animals with OJ (n= 20); 3) IM, infarcted animals with water and maltodextrin (n= 40); and 4) IOJ, infarcted animals with OJ (n = 40). Statistical analysis was performed by the two-way ANOVA supplemented by Holm-Sidak. Results are presented as mean ± standard deviation, the level of significance adopted was 5%. Results After 3 months, MI led to left ventricular (LV) hypertrophy, with systolic and diastolic dysfunction, and increased oxidative stress and inflammatory mediators. OJ intake reduced LV cavity and improved systolic and diastolic function. The OJ animals presented lower activity of glutathione peroxidase and higher expression of heme-oxygenase-1 (HO-1). Conclusion OJ attenuated CR in infarcted rats and HO-1 may be play an important role in this process.


Subject(s)
Animals , Male , Rats , Citrus sinensis , Myocardial Infarction , Systole , Ventricular Remodeling , Heart
18.
Semina cienc. biol. saude ; 42(2): 127-134, jun./dez. 2021. Tab
Article in Portuguese | LILACS | ID: biblio-1292795

ABSTRACT

Objetivo: verificar a presença de compostos bioactivos em papas principais, recomendadas para alimentação complementar. Métodos: a amostra foi constituída por seis preparações de papas principais, escolhidas aleatoriamente, cujas receitas são encontradas em dois manuais de orientação alimentar infantil, veiculados no Brasil. Análise dos compostos bioactivos, com base nos compostos fenólicos totais, ácido ascórbico, carotenoides e a concentração de vitamina A pré-formada de cada papa, foi realizada. Resultados: os teores de compostos fenólicos variaram entre 25,8 mg e 33,8 mg/100 g. Os níveis de ácido ascórbico apresentaram valores entre 10,8 mg/100 g e 87,8 mg/100 g. Em relação ao conteúdo total de carotenoides, encontrou-se valores entre 7,4 µg/100 g a 177,8 µg/100 g. Quanto à presença de vitamina A, os valores obtidos variaram de 266 e 322 µg/100 g. Conclusão: as papas principais propostas nesses manuais apresentam quantidade significativa de compostos bioactivos.


Objective: to verify the presence of bioactive compounds in baby foods recommended for complementary feeding. Methods: the sample consisted of six baby foods preparations, chosen at random, whose recipes are found in two manuals for infant food guidance, published in Brazil. Analysis of bioactive compounds, based on total phenolic compounds, ascorbic acid, carotenoids and the preformed vitamin A concentration of each baby foods were also evaluated. Results: the contents of phenolic compounds ranged from 25.8 mg to 33.8 mg/100 g. The levels of ascorbic acid showed values between 10.8 mg/100 g and 87.8 mg/100 g. Regarding the total carotenoid content, values were found between 7.4 µg/100 g to 177.8 µg/100 g. Regarding the presence of vitamin A, the values obtained ranged from 266 and 322 µg/100 g. Conclusion: the baby foods proposed in these manuals, present a significant amount of bioactive compounds


Subject(s)
Infant , Infant , Infant Nutritional Physiological Phenomena , Antioxidants
19.
Arq. bras. med. vet. zootec. (Online) ; 73(3): 721-732, May-June 2021. tab
Article in English | LILACS, VETINDEX | ID: biblio-1278356

ABSTRACT

This study aims to evaluate effects of inclusion of moringa in Japanese quail diets on laying performance, egg quality, blood parameters, serum biochemical profile, and behavior. One hundred and forty-four Japanese quails, approximately 35-d-old, were distributed in a completely randomized design with four treatments and six replications. Treatments were diet inclusion of 0, 2, 4 and 6% of dried and ground moringa leaves. The inclusion of moringa in diets reduces the quadratic feed intake up to the level of 1.20%, increases weight of eggs with a quadratic behavior up to 3.80%, and linearly increases yolk weight. Yolk color changes with higher levels of inclusion of moringa and resulted in more intense colors. The biochemical profile of quails changed slightly but remains within the normal range. The inclusion of 4% of moringa in diets increases alkaline phosphatase. Regarding cholesterol and triglycerides, diet with 6% moringa inclusion was lower when compared to the others. The behavior of laying quails does not change due to inclusion of moringa in diets. Up to 3.83% of Moringa oleifera can be included in Japanese quail diet to improve egg quality without compromising performance, biochemical profile, blood parameters and behavior.(AU)


O objetivo deste estudo foi avaliar a inclusão de Moringa oleifera na dieta de codornas japonesas sobre o desempenho, a qualidade de ovos, os parâmetros sanguíneos, o perfil bioquímico e o comportamento. Foram utilizadas 144 codornas japonesas, com aproximadamente 35 dias de idade, as quais foram distribuídas em delineamento inteiramente ao acaso, com quatro tratamentos e seis repetições cada. Foram fornecidas dietas com 0, 2, 4 e 6% de folhas desidratadas e moídas de moringa. A inclusão de moringa nas dietas reduziu quadraticamente o consumo até o nível de 1,2%, aumentou o peso dos ovos com comportamento quadrático até o nível de 3,8% e aumentou linearmente o peso da gema. A coloração da gema se intensificou com maiores inclusões de moringa. O perfil bioquímico sofreu alterações leves, mas não saiu dos padrões normais para codornas. A inclusão de 4% de moringa nas dietas aumentou os níveis de fosfatase alcalina. Já a inclusão de 6% reduziu níveis de colesterol e triglicérides. O comportamento das codornas não se alterou com a inclusão de moringa nas dietas. A inclusão de até 3,83% de Moringa oleifera na dieta de codornas japonesas aumenta a qualidade de ovos sem comprometer o desempenho, o perfil bioquímico, os parâmetros sanguíneos e o comportamento delas.(AU)


Subject(s)
Animals , Coturnix , Moringa oleifera , Eggs/analysis , Phytochemicals/therapeutic use , Plants, Medicinal
20.
Revista Digital de Postgrado ; 10(1): 280, abr. 2021. tab
Article in Spanish | LILACS, LIVECS | ID: biblio-1147585

ABSTRACT

Se evaluó la composición proximal, el contenido de polifenoles totales y la capacidad antioxidante de un chocolate oscuro formulado a partir de granos de cacao orgánico, seco, y no sometido al proceso fermentativo. Este producto fue elaborado con insumos y procesos desarrollados en la Granja Experimental El Cairo, ubicada en la Universidad Nacional de Colombia (GEL-UN), departamento de Arauca, Colombia. El contenido de grasa se encontró entre los rangos conocidos (>50%), siendo una característica ligada al origen genético, y a las condiciones climáticas. El alto contenido de proteínas (16,21%), evidencia que se trata de granos de cacao secos no fermentados. Tal contenido juega un papel importante en la formación de los precursores del sabor y aroma, en chocolates finos y exquisitos. El pH determinado es un indicativo de un chocolate amargo, con bajo índice de fermentación y de baja acidez acética o láctica. El contenido de polifenoles totales se relaciona de manera directa con la alta actividad antioxidante de este chocolate. Se concluye que el tipo de material genético usado como insumo, así como los procesos implementados para la obtención del chocolate, en GEL-UN, contribuyeron para la obtención de un producto tipo comercial, con propiedades funcionales, entre ellas las relacionadas con la capacidad antioxidante(AU)


Thee proximal chemical composition, the content of total polyphenols, and the antioxidant capacity of a dark chocolate formulated from organic, dry cocoa beans and not subjected to the fermentation process were evaluated. This product was made with inputs and processes developed at the El Cairo Experimental Farm, located at the Universidad Nacional de Colombia (CEF-UN), Arauca, Colombia. The fat content was found between the known ranges (> 50%), being a characteristic linked to the genetic origin, and to the climatic conditions. The high protein content (16.21%) evidenced that it´s unfermented dry cocoa beans. Such content plays an important role in the formation of precursors of flavor and aroma, in fine and exquisite chocolates. The determined pH is indicative of a dark chocolate, with a low fermentation index and low acetic or lactic acidity. The content of total polyphenols is directly related to the high antioxidant activity of this chocolate. It is concluded that the type of genetic material used as input, as well as the processes implemented to obtain chocolate, in CEF-UN, contributed to obtaining a commercial type product, with functional properties, including those related to antioxidant capacity(AU)


Subject(s)
Cacao/chemistry , Polyphenols/analysis , Chocolate/analysis , Antioxidants/analysis , Fermentation , Food, Organic , Food Handling
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