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Article in Chinese | WPRIM | ID: wpr-852177

ABSTRACT

Objective To establish the method of simultaneous determination of the content of 13 nucleosides and nucleobases, including cytosine, uracil, adenine, guanine, 6-hydroxypurine, 2,6-dihydroxypurine, uridine, thymine, inosine, guanosine, adenosine, 2′-deoxyguanosine (2′-dG), beta-thymidine, in Cervi Cornu Pantotrichum (CCP) of sika deer (Cervus nippon) by UPLC, and compare the distribution differences of nucleosides and nucleobases in different zones of the CCP with different processing methods. Methods The nucleosides and nucleobases in CCP were extracted by water with assistance of ultrasound. Acquity UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm) was used as chromatographic column to separate the nucleosides and nucleobases. Thirteen target compounds were eluted with acetonitrile 100% (eluent A) and water plus 0.006% formic acid (eluent B) at a flow rate of 0.3 mL/min. The column temperature was 30 ℃, and the injection volume was 3 μL and the detection wavelength was 260 nm. Results A total of 13 nucleosides and nucleobases basically reached the baseline separation with a good linearity within linear range (r > 0.999 6). The nucleosides and nucleobases content in wax slices, powder slices, gauze slices of CCP without and with blood were respectively 4.47, 3.95, 2.68 g/kg and 4.14, 3.44, 2.51 g/kg. And those three parts of CCP with boiling and freeze-drying processing were respectively 4.60, 2.95, 2.74 g/kg and 5.06, 4.24, 2.31 g/kg. Conclusion As far as the total content of nucleosides and nucleobases were concerned, the wax slices, powder slices and gauze slices of CCP without blood were all higher than those of CCP with blood. The wax slices, powder slices of CCP with freeze-drying processing were more than those of CCP with boiling processing, while the gauze slices of which were less than those of CCP with boiling processing.

2.
Article in English | WPRIM | ID: wpr-812547

ABSTRACT

Boiling processing is commonly used in post-harvest handling of White Paeony Root (WPR), in order to whiten the herbal materials and preserve the bright color, since such WPR is empirically considered to possess a higher quality. The present study was designed to investigate whether and how the boiling processing affects overall quality of WPR. First, an ultra-high performance liquid chromatography quadrupole/time-of-flight mass spectrometry-based metabolomics approach coupled with multivariate statistical analysis was developed to compare the holistic quality of boiled and un-boiled WPR samples. Second, ten major components in WPR samples boiled for different durations were quantitatively determined using high performance liquid chromatography to further explore the effects of boiling time on the holistic quality of WPR, meanwhile the appearance of the processed herbal materials was observed. The results suggested that the boiling processing conspicuously affected the holistic quality of WPR by simultaneously and inconsistently altering the chemical compositions and that short-time boiling processing between 2 and 10 min could both make the WPR bright-colored and improve the contents of major bioactive components, which were not achieved either without boiling or with prolonged boiling. In conclusion, short-term boiling (2-10 min) is recommended for post-harvest handling of WPR.


Subject(s)
Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Chemistry , Reference Standards , Hot Temperature , Mass Spectrometry , Methods , Paeonia , Chemistry , Plant Roots , Chemistry , Technology, Pharmaceutical , Water
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