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1.
Braz. j. biol ; 84: e255529, 2024. tab, graf
Article in English | LILACS-Express | MEDLINE, LILACSEXPRESS, LILACS, VETINDEX | ID: biblio-1364534

ABSTRACT

Abstract Reports from popular medicine usually act as a basis for the development of new drugs from natural compounds with therapeutic actions for serious diseases and prevalence such as cancer. Bromelia antiacantha Bertol. is a species of the Bromeliaceae family, considered an unconventional food plant, found in the south and midwest regions of Brazil. Despite the high nutritional content and pharmacological potential of its fruits, few scientific studies report its biological actions. Thus, this study evaluates the phytochemical profile of aqueous and ethanol extracts obtained from B. antiacantha fruits, as well as their possible antioxidant, antitumor, and cytotoxic activities. The aqueous extract exhibited phenolic compounds and flavonoids, while ethanol extracts indicated the presence of flavonoids and coumarin in their composition, regardless of the region of collection. The ethanolic extract demonstrated a more promising antioxidant effect than the aqueous extract and also induced a significant inhibition in the viability of human cervical cancer cells of the SiHa strain. In addition, treatment with both extracts did not alter the viability of non-tumor cells of the immortalized human keratinocyte lineage (HaCaT). These results bring new data about extracts obtained from a native plant, edible and traditionally used in popular medicine, opening new perspectives for its possible therapeutic application.


Resumo Relatos da medicina popular costumam atuar como referencial para o desenvolvimento de novos fármacos a partir de moléculas naturais com ações terapêuticas para doenças de alta gravidade e prevalência como o câncer. Bromelia antiacantha Bertol. é uma espécie da família Bromeliaceae, considerada uma planta alimentícia não convencional (PANC), encontrada nas regiões sul e centro-oeste do Brasil. Apesar do alto teor nutritivo e potencial farmacológico de seus frutos, poucos estudos científicos relatam suas ações biológicas. Desta forma, este estudo avalia o perfil fitoquímico de extratos aquoso e etanólico obtidos de frutos de B. antiacantha, bem como a sua possível ação antioxidante, antitumoral e citotóxica. O extrato aquoso apresentou compostos fenólicos e flavonoides, enquanto os extratos etanólicos apontam a presença de flavonóides e cumarina em sua composição, independente da região de coleta. O extrato etanólico demonstrou efeito antioxidante mais promissor do que o extrato aquoso e também induziu uma inibição significativa na viabilidade de células humanas de câncer cervical da linhagem SiHa. Além disso, o tratamento com ambos extratos não alterou a viabilidade de células não tumorais da linhagem de queratinócitos humanos imortalizados (HaCaT). Estes dados trazem novas informações sobre extratos obtidos de uma espécie vegetal nativa, comestível e já utilizada tradicionalmente, mas abrindo novas perspectivas quanto a possíveis aplicações terapêuticas.

2.
Säo Paulo med. j ; 140(3): 349-355, May-June 2022. tab, graf
Article in English | LILACS | ID: biblio-1377377

ABSTRACT

ABSTRACT BACKGROUND: Cervical cancer is a type of cancer caused by human papillomavirus (HPV). OBJECTIVE: To determine the relationship between awareness of cervical cancer and HPV infection and attitudes towards HPV vaccine among women aged 15-49 years. DESIGN AND SETTING: Cross-sectional study conducted at Karabük Training and Research Hospital, Turkey. METHODS: 500 women who visited the gynecology outpatient clinic of a public hospital between July 15 and December 31, 2019, were selected through random sampling. Data were collected using a sociodemographic questionnaire comprising nine questions (created by the researchers), the HPV and Cervical Cancer Awareness Questionnaire and the Carolina HPV Immunization Attitudes and Beliefs Scale. RESULTS: The relationship between the awareness questionnaire and the beliefs scale was explained through simple effect modeling of a structural equation. The women's knowledge score regarding cervical cancer and HPV infection was 4.69 ± 4.02 out of 15. Women were afraid of being diagnosed with cervical cancer and HPV infection, but they did not have sufficient information. They had poor information about the HPV vaccine, did not know how to obtain the vaccine and did not have enough information about its benefits and harmful effects. Women who were afraid of getting cervical cancer, and who thought that they were at risk, had more information about the HPV vaccine. CONCLUSION: Women need information about cervical cancer, HPV infection and the HPV vaccine. Midwives, nurses and physicians who provide healthcare services in gynecological follow-ups should provide information to women about the HPV vaccine and cervical cancer.


Subject(s)
Uterine Cervical Neoplasms/prevention & control , Papillomavirus Infections , Papillomavirus Vaccines , Health Knowledge, Attitudes, Practice , Cross-Sectional Studies , Surveys and Questionnaires
3.
Medicina (B.Aires) ; 82(1): 142-146, feb. 2022. graf
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1365139

ABSTRACT

Resumen Una mujer de 40 años se internó de forma programada para recibir quimioterapia por una leucemia linfoblástica aguda (LLA) B común de alto riesgo, diagnosticada 10 meses antes a raíz de hemato mas y petequias en los miembros inferiores, y metrorragia. En ese momento, presentaba trombocitopenia y una ecografía ginecológica transvaginal normal. Al ingreso de la internación programada, se quejó de dolor inguinal izquierdo con irradiación al muslo e impotencia funcional de 3 meses de evolución, dolor en fosa ilíaca derecha y diarrea. En el examen físico tenía dolor a la palpación profunda en la fosa ilíaca derecha y signo del psoas positivo a la izquierda. La tomografía de abdomen y pelvis reveló una imagen compatible con un absceso del psoas izquierdo y signos de tiflitis. La biopsia del psoas izquierdo demostró infiltración por nidos y cordones de carcinoma escamoso queratinizante moderadamente diferenciado. El examen ginecológico dirigido evidenció anomalías macroscópicas del cuello uterino correlacionadas con el mismo diagnóstico histopatológico. Los se gundos cánceres primarios más frecuentemente asociados a LLA son linfoma de Hodgkin, cáncer escamoso de piel, tumores endocrinos, cáncer renal, linfoma no-Hodgkin y cáncer de mama. Las metástasis musculares de tumores sólidos son infrecuentes, y habitualmente provienen del pulmón, riñón, tiroides y melanoma. El síndrome del psoas maligno es causado por infiltración neoplásica del músculo. El diagnóstico diferencial debe realizarse con el absceso del psoas, que puede originarse en una tiflitis si es secundario. No hemos podido encontrar registros de cáncer de cuello uterino como segundo cáncer primario luego de LLA.


Abstract A 40-year-old woman was scheduled to receive chemotherapy for a high-risk common B-cell acute lymphoblastic leukemia (ALL), diagnosed 10 months earlier in the wake of lower limb bruising and petechiae, and metrorrhagia. At that time, she had thrombocytopenia and a normal transvaginal gynecological ultrasound. Upon admission, she complained of a 3-month history of incapacitating left groin pain radiated to the thigh, and right lower quadrant abdominal pain associated with diarrhea. On physical examination, she had tenderness in the right iliac fossa and a positive psoas sign on the left. Computerized scan of the abdomen and pelvis reported an image compatible with a left psoas abscess and signs of typhlitis. The biopsy of the left psoas muscle demonstrated infiltration by nests and cords of moderately differentiated keratinizing squamous carcinoma. Gynecological examination revealed macroscopic abnormalities of the cervix correlated with the same histopathological diagnosis. The second primary cancers most frequently associated with ALL are Hodgkin lymphoma, squamous skin cancer, endocrine tumors, kidney cancer, non-Hodgkin lymphoma, and breast cancer. Muscle metastases from solid tumors are rare, and usually arise from the lung, kidney, thyroid, and melanoma. Malignant psoas syndrome is caused by neoplastic infiltration of the muscle. The differential diagnosis should be made with a psoas abscess, which may arise from typhlitis if secondary. We have not been able to find records of cervical cancer as second primary cancer after ALL.

4.
Article in Chinese | WPRIM | ID: wpr-920391

ABSTRACT

Objective To understand the HPV vaccination situation in the vaccination clinic of Shaanxi Provincial Center for Disease Control and Prevention, and to guide the rational use of the vaccine. Methods The vaccination information and the information on HPV vaccine inoculated subjects in the clinic of Shaanxi Provincial Center for Disease Control and Prevention from 2018 to 2019 were analyzed. Results A total of 5 714 HPV vaccination subjects were analyzed, among which the largest proportion (48.97%) was in the 20-26.5 years old group, and the smallest proportion (1.12%) was in the 9-15 years old group. The 9-valent HPV vaccination accounted for 98.72% of the 20-26.5-year-old group. There were statistically significant differences in the distribution of vaccination populations between the bivalent and quadrivalent HPV vaccines (P<0.01,χ2=252.85), and between the bivalent and 9-valent HPV vaccines (P<0.01,χ2=258.15). The vaccination rate of the bivalent HPV vaccine was 88.25% (894/1 013), and the vaccination rate of the quadrivalent HPV vaccine was 94.43% (1 915/2 028). The difference was statistically significant (Z=-2.02, P=0.043). Conclusion Vaccination subjects tend to choose high-valent HPV vaccines, and the proportion of HPV vaccination in the younger age group is seriously insufficient. Both bivalent and quadrivalent HPV vaccines have relatively high overall vaccination rates. The next step should be to strengthen the vaccination publicity for younger age groups.

5.
Esc. Anna Nery Rev. Enferm ; 26: e20210137, 2022. tab, graf
Article in Portuguese | LILACS, BDENF | ID: biblio-1350740

ABSTRACT

Resumo Objetivo avaliar o tempo de atuação de médicos e enfermeiros na Atenção Primária à Saúde (APS) e qualidade das ações desenvolvidas para controle do câncer cervicouterino (CC). Métodos estudo transversal, conduzido de janeiro a março de 2019 em região de saúde compreendida em 19 municípios localizada no estado da Bahia, Brasil. A amostra foi de 241 médicos e enfermeiros da APS. Utilizou-se a linha de cuidado do CC como condição traçadora. Elegeram-se o desfecho tempo de atuação na APS no mesmo município, categorizado em < 2 anos e ≥ 2 anos, e indicadores representativos da qualidade da APS. Os testes χ2 de Pearson e exato de Fisher foram empregados. Resultados a prevalência de tempo de atuação na APS foi 43,57% (IC95%: 37,40%; 49,94%) para < 2 anos e 56,43% (IC95%: 50,06%; 62,60%) para ≥ 2 anos. Observaram-se maiores prevalências, com diferença estatística significativa, dos indicadores de qualidade para o maior tempo de atuação. Conclusões e implicações para a prática a rotatividade profissional parece afetar o cuidado longitudinal de mulheres na linha de cuidado eleita. Sugere-se a ampliação do número e do papel dos enfermeiros, especialmente nos serviços de APS, para maior resolutividade e eficiência do sistema de saúde.


Resumen Objetivo evaluar el tiempo de actuación de médicos y enfermeros en la Atención Primaria de Salud (APS) y la calidad de las acciones desarrolladas para el control del cáncer cérvicouterino (CC). Métodos estudio transversal realizado de enero a marzo de 2019, en una región sanitaria que comprende 19 municipios en el estado de Bahía, Brasil. La muestra fue de 241 médicos y enfermeros de APS. La línea de cuidados de CC fue la condición trazadora. Se eligió el resultado tiempo trabajando en APS en el mismo municipio, categorizado en < 2 años y ≥ 2 años e indicadores representativos de calidad de APS. Se utilizaron pruebas exactas de chi-cuadrado de Pearson y Fisher. Resultados la prevalencia del tiempo de actuación en APS fue del 43,57% (IC95%: 37,40%; 49,94%) para < 2 años y del 56,43% (IC95%: 50,06%; 62,60%) para ≥ 2 años, considerado incipiente. Se observó una mayor prevalencia, con diferencia estadísticamente significativa, de los indicadores de calidad para un mayor tiempo de actuación. Conclusiones e implicaciones para la práctica la rotación de profesionales parece afectar la atención longitudinal de las mujeres en la línea de cuidado elegida. Se sugiere la ampliación del número y el papel de enfermería, especialmente en los servicios de APS, para una mayor resolutividad y eficiencia del sistema sanitario.


Abstract Objective to assess the working length of physicians and nurses in Primary Health Care (PHC) and the quality of actions taken to control cervical cancer (CC). Methods this is a cross-sectional study, conducted from January to March 2019, in a health region comprised of 19 municipalities located in the state of Bahia, Brazil. The sample consisted of 241 PHC physicians and nurses. The CC care line was used as a tracer condition. The outcome experience length in PHC in the same municipality was chosen, categorized as < 2 years and ≥ 2 years and representative indicators of PHC quality. Pearson's χ2 and Fisher's exact tests were used. Results the prevalence of length of experience in PHC was 43.57% (95%CI: 37.40%; 49.94%) for < 2 years, and 56.43% (95%CI: 50.06%; 62.60%) for ≥ 2 years. There was a higher prevalence, with a statistically significant difference, of the quality indicators for the longest working length. Conclusions and implications for practice professional turnover seems to affect the longitudinal care of women in the chosen care line. It is suggested to expand the number and role of nurses, especially in PHC services, for greater resolution and efficiency of the health system.


Subject(s)
Humans , Male , Female , Adult , Primary Health Care , Quality of Health Care/statistics & numerical data , Health Evaluation/statistics & numerical data , Uterine Cervical Neoplasms/prevention & control , Health Personnel , Mass Screening , Cross-Sectional Studies , Papanicolaou Test
6.
Infectio ; 25(4): 270-275, oct.-dic. 2021. tab, graf
Article in Spanish | LILACS, COLNAL | ID: biblio-1286721

ABSTRACT

Resumen Objetivo: Comparar la expresión de mRNA y proteínas de SFRP1, PTPRN, CDO1, EDNRB, CDX2, EPB41L3 y HAND1 en pacientes con lesión intra-epitelial cervical de bajo y alto grado, con posterior progresión o regresión. Material y Método: Se realizó análisis de expresión de genes mediante RT-PCR y análisis de expresión de proteínas por inmunohistoquímica. El análisis estadís tico fue realizado con las pruebas: Wilcoxon, coeficiente de correlación de Spearman e índice de concordancia. Las muestras fueron pareadas en momento 1 y momento 2. Resultados: SFRP1 mostró tendencia de mayor expresión de mRNA en lesión intra-epitelial de bajo grado (momento 2) Vs. alto grado (momento 1). La expresión de proteínas por inmunohistoquímica de SFRP1 en casos de progresión (83,3 %) mostró disminución en su graduación (p = 0,0313*); los demás genes en estudio no tuvieron cambios estadísticamente significativos. Discusión: SFRP1 mostró comportamiento ajustado a resultados de estudios previos donde se encontró hipermetilado en lesiones intra-epiteliales de alto grado; su subexpresión por hipermetilación se reportó en otros canceres, proceso que colabora con su silenciamiento y transición epitelial-mesenquimatosa del cáncer de cuello uterino. Conclusiones. SFRP1 es potencial biomarcador en lesiones preneoplásicas del cuello uterino asociadas al virus de papiloma humano.


Abstract Objective. The aim of this work was to compare the expression of mRNA and proteins of SFRP1, PTPRN, CDO1, EDNRB, CDX2, EPB41L3 and HAND1 in patients with low and high grade cervical intraepithelial lesion, with subsequent progression or regression. Material and Methods: Gene expression analysis was conducted through RT-PCR and protein expression analysis was performed by immunohistochemistry. The statistics analysis were Wilcoxon test, Spearman's correlation coefficient and concordance index. The samples were paired during moment 1 (initial patient diag nosis) and moment 2 (follow-up histological diagnosis). Results: SFRP1 showed a trend of higher mRNA expression in low-grade intra-epithelial lesions (moment 2) Vs. high-grade (moment 1). The expression of proteins by immunohistochemistry of SFRP1 in progression cases (83.3%) showed a decrease in its graduation (p = 0.0313*); the other genes under study had no statistically significant. Discussion: SFRP1 showed a biological behavior adjusted to the results of previous studies where hypermethylation was found in high-grade intra-epithelial lesions; its subexpression by hypermethylation has been reported in other cancers, a process that collaborates with its silencing and epithelial-mesenchymal tran sition of cervical. Conclusions. SFRP1 is a potential biomarker in preneoplastic lesions of the cervix associated with human papillomavirus.


Subject(s)
Humans , Female , Adult , Papilloma , DNA Probes, HPV , Viruses , Proteins , Uterine Cervical Neoplasms , Disease Progression , Alphapapillomavirus
7.
Ciênc. Saúde Colet ; 26(10): 4497-4509, out. 2021. tab, graf
Article in English, Portuguese | LILACS | ID: biblio-1345699

ABSTRACT

Resumo Este artigo analisa as políticas e ações de controle do câncer do colo do útero no Brasil e no Chile, com foco na prevenção e no rastreamento. Adotou-se a abordagem comparativa, buscando identificar semelhanças e diferenças entre as diretrizes e as estratégias de prevenção e rastreamento do câncer do colo do útero entre os países. A pesquisa compreendeu revisão bibliográfica, análise documental e de dados secundários e consultas a especialistas, técnicos e dirigentes do programa. Em que pesem as diferenças nas estratégias de rastreamento do câncer do colo do útero, o Chile possui um programa bem estruturado, com concentração de decisões no nível nacional e um sistema que permite o monitoramento das ações. O Brasil enfrenta recorrentes problemas relacionados à falta de uma coordenação das ações e falhas no segmento das mulheres com exames alterados. As principais dificuldades para a consolidação de programas de rastreamento do câncer do colo do útero (não realização da busca ativa da população em risco, ausência de sistema de controle de qualidade dos exames e seguimento inadequado de mulheres com resultados alterados) são mais evidentes no Brasil. Em ambos os países há necessidade de aumento da cobertura e implantação do rastreamento organizado.


Abstract This article analyzes cervical cancer control policies and actions in Brazil and Chile, focusing on prevention and screening. We adopted a comparative approach to identify similarities and differences in guidelines and cervical cancer prevention and screening strategies between the two countries. We used the following data collection techniques: analysis of official documents and secondary data, consultations with experts, government officials and program coordinators, and literature review. The findings show that Chile has a well-structured program with centralized decision-making and a system that permits monitoring of actions. Brazil on the other hand faces ongoing issues with lack of coordination and shortcomings in the follow-up of women with abnormal test results. The following challenges to consolidating cervical cancer screening stand out in Brazil: lack of active tracking of the target population; absence of a test quality assurance system; and inadequate follow-up of women with abnormal test results. Both countries need to increase coverage and implement organized screening.


Subject(s)
Humans , Female , Uterine Cervical Neoplasms/diagnosis , Uterine Cervical Neoplasms/prevention & control , Brazil , Chile , Mass Screening , Early Detection of Cancer
8.
Bol. latinoam. Caribe plantas med. aromát ; 20(4): 394-405, jul. 2021. ilus
Article in English | LILACS | ID: biblio-1352427

ABSTRACT

In this study, it was aimed to determine the antioxidant and anticancer activities of Sideritis perfoliata methanolic extract (SPE) on cervical cancer cells (HeLa). Different doses (25, 50,100 and 200 µg/mL) of SPE were used to determine proliferation of HeLa cells by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) staining method. Induction of apoptosis was determined by Annexine-V and propidium iodide staining method. Interleukin (IL) 6-8 levels were measured by ELISA method. Antioxidant activities of SPE were determined by DPPH, DNA (plasmid pBR322) protecting and cellular antioxidant activity tests. Some phytochemicals of SPE were also screened by LC-MS-MS. It was determined that SPE reduced the proliferation of HeLa cells and also induced apoptosis. IL6-8 levels importantly decreased at 200 µg/mL. SPE exhibited moderately antioxidant activities in tests used. Among the phenolics identified, vanillic acid had the highest amount. As a result, it was determined to have the anticancer activity of SPE by decreasing cell proliferation, inducing apoptosis and decreasing IL6-8 in HeLa cells.


En este estudio, se tuvo como objetivo determinar las actividades antioxidantes y anticancerígenas del extracto metanólico de Sideritis perfoliata (SPE) en las células de cáncer de cuello uterino (HeLa). Se utilizaron diferentes dosis (25, 50, 100 y 200 µg/mL) de SPE para determinar la proliferación de células HeLa mediante el método de tinción con bromuro de 3-[4,5-dimetiltiazol-2-il] -2,5-difenil-tetrazolio (MTT). La inducción de apoptosis se determinó mediante el método de tinción con anexina-V y yoduro de propidio. Los niveles de interleucina (IL) 6-8 se midieron mediante el método ELISA. Las actividades antioxidantes de SPE se determinaron mediante pruebas de DPPH, protección de ADN (plásmido pBR322) y actividad antioxidante celular. Algunos fitoquímicos de SPE también se analizaron mediante LC-MS-MS. Se determinó que SPE redujo la proliferación de células HeLa y también indujo apoptosis. Los niveles de IL6-8 disminuyeron de manera importante a 200 µg/mL. SPE mostró actividades moderadamente antioxidantes en las pruebas utilizadas. Entre los fenólicos identificados, el ácido vainílico tuvo la mayor cantidad. Como resultado, se determinó que tenía la actividad anticancerígena de SPE al disminuir la proliferación celular, inducir apoptosis y disminuir la IL6-8 en las células HeLa.


Subject(s)
Plant Extracts/administration & dosage , Uterine Cervical Neoplasms , Sideritis/chemistry , Cell Proliferation/drug effects , Antioxidants/administration & dosage , Phenols/analysis , Plant Extracts/chemistry , Cell Survival , Interleukin-8/analysis , Interleukin-6/analysis , Apoptosis/drug effects , Gas Chromatography-Mass Spectrometry , Antineoplastic Agents , Antioxidants/chemistry
9.
Femina ; 49(7): 444-448, 20210731. tab
Article in Portuguese | LILACS | ID: biblio-1290595

ABSTRACT

A exenteração pélvica pode curar pacientes com câncer de colo do útero com recorrência central após radioterapia e quimioterapia. A avaliação pré-operatória é essencial para excluir doença metastática e evitar cirurgias desnecessárias nesse cenário. O objetivo do presente estudo é avaliar a sobrevida de uma série de casos de pacientes submetidas à exenteração pélvica em clínica privada de Teresina. Este é o resultado parcial de um estudo observacional, retrospectivo, transversal e descritivo, realizado em uma clínica privada especializada no tratamento do câncer em Teresina, PI, Brasil, de junho de 2002 a fevereiro de 2020. Cinco pacientes foram incluídas no estudo, com idades entre 29 e 62 anos. No presente estudo, a sobrevida mediana foi de 44,8 meses. Duas pacientes estão vivas e sem doença com seguimento de 201 e 5 meses, respectivamente.(AU)


Pelvic exenteration can heal patients with cervical cancer with central recurrence after radiotherapy and / or chemotherapy. Preoperative evaluation is essential to exclude metastatic disease and to avoid unnecessary surgery in this scenario. The objective of the present study is to evaluate the survival of a series of cases of patients submitted to pelvic exenteration in a private clinic in Teresina. This is the partial result of an observational, retrospective, cross-sectional and descriptive study, conducted at a private clinic specialized in cancer treatment in Teresina, Brazil, from June 2002 to February 2020. Five patients were included in the study, aged between 29 and 62 years. In the present study, the median survival was 44,8 months. Two patients are alive and without disease with a follow-up of 201 and 5 months, respectively.(AU)


Subject(s)
Humans , Female , Pelvic Exenteration/statistics & numerical data , Survival Analysis , Uterine Cervical Neoplasms/surgery , Neoplasm Recurrence, Local/epidemiology , Survival , Brazil/epidemiology , Cross-Sectional Studies
10.
Rev. med. Risaralda ; 27(1): 21-27, ene.-jun. 2021. graf
Article in Spanish | LILACS, COLNAL | ID: biblio-1280489

ABSTRACT

Resumen Objetivo: Analizar la tendencia de la incidencia de cáncer de cuello uterino invasivo en mujeres entre 20 y 39 años en Manizales, Colombia, 2003-2018. Materiales y métodos: Estudio observacional, descriptivo, retrospectivo, de base poblacional. Se evaluaron las tendencias temporales en la incidencia (con referencia a Población Segi, por 100.000 mujeres/año) utilizando la regresión de Joinpoint (Joint Point Regression Program v. 4.7.0.0 del National Cancer Institute de los EEUU) para estimar los cambios porcentuales anuales y años de variación significativa en las tendencias. Resultados: Se realizó el estudio con un total de 116 pacientes, cuya edad promedio al momento del diagnóstico fue 33.7 años. Sólo se hallaron 3 casos de cáncer invasivo en mujeres entre 20 y 25 años. El mayor número de casos se presentó en el grupo de mujeres de 35 a 39 años. El tipo histológico más frecuente fue el carcinoma escamocelular. El 45.6% de los casos se diagnosticaron en etapas tempranas. El Cambio Porcentual Anual Promedio calculado mediante el modelo de regresión Joinpoint mostró un descenso de 7,9% en la tasa de incidencia entre 2003 y 2018, con un punto de quiebre en el año 2010. Conclusiones: El cáncer de cuello uterino en Manizales tiene escasa frecuencia en mujeres menores de 25 años y para edades entre 20 y 39 años muestra una tendencia a la disminución en el tiempo, en particular desde el año 2010 en adelante.


Abstract Objective: This article analyzes the invasive uterine cancer occurrences in women between 20 and 39 years old in Manizales, Colombia, 2003-2018. Materials and methods: Observational, descriptive, retrospective, population-based study. Temporary trends of occurrences were evaluated (as referring to Population Segi, per 100,000 women / year) by using the Joinpoint regression (Joint Point Regression Program v. 4.7.0.0 of the National Cancer Institute of the USA), in order to estimate annual percentage changes and number of years of significant variation in trends. Results: The study was carried out with a total of 116 patients, whose average age at the time of diagnosis was 33.7 years. Only 3 invasive cancer cases were found in women between the ages of 20 and 25. The greatest number of cases occurred in the group of women between 35 and 39 years old. The most frequent histological type was squamous cell carcinoma. 45.6% of cases were diagnosed in early stages. The Average Annual Percentage Change calculated using the Joinpoint regression model showed a 7.9% decrease in the incidence rate between 2003 and 2018, with a breaking point in 2010. Conclusions: Cervical cancer in Manizales is infrequent in women under the age of 25; and, for ages between 20 and 39, it has shown a tendency to decrease over time, particularly from 2010 onwards.


Subject(s)
Humans , Female , Adult , Uterine Neoplasms , Uterine Cervical Neoplasms , Carcinoma, Squamous Cell , Colombia , Diagnosis , Joints , Methods , Neoplasms
11.
Rev. medica electron ; 43(1): 2784-2794, tab
Article in Spanish | LILACS, CUMED | ID: biblio-1156775

ABSTRACT

RESUMEN Introducción: el cáncer cérvico uterino constituye un problema de salud para la humanidad. El estudio de sus factores de riesgo representa prioridad para el sistema de salud cubano. Objetivo: conocer el comportamiento de los factores de riesgo del cáncer cérvico uterino, en estudiantes de las carreras de Higiene-Epidemiología-Vigilancia y Lucha Antivectorial de la Universidad de Ciencias Médicas de Matanzas. Materiales y métodos: investigación descriptiva, transversal, retrospectiva que se insertó en el Programa Ramal Enfermedades Crónicas no Transmisibles. Se trabajó con el universo de 50 alumnos de las carreras de Higiene- Epidemiología-Vigilancia y Lucha Antivectorial de la Universidad de Ciencias Médicas de Matanzas. Resultados: según edad de inicio de las relaciones sexuales, se observó mayor predominio a los 14 años de edad. Presencia de múltiples compañeros sexuales, relaciones sexuales desprotegidas, uso de tabletas anticonceptivas, hábito de fumar, manipulaciones ginecológicas, así como infecciones de transmisión sexual. Especial significación tuvo la evidencia de cifras significativas de múltiples compañeros sexuales y el uso de tabletas anticonceptivas. Entre la distribución de motivos que impulsaron al inicio de relaciones sexuales, se destacaron los de experimentar sensaciones nuevas con el 28 %, y búsqueda de placer con la pareja en 55 %. El 92 %, desconocían los factores de riesgo de la enfermedad. Conclusiones: los estudiantes presentaron factores de riesgo de considerable magnitud de padecer cáncer cérvico uterino (AU).


SUMMARY Introduction: cervical cancer is a health problem for the humankind. The study of its risk factors stands as a priority for the Cuban health system. Objective: to know the behavior of cervical cancer risk factors in students of the studies of Hygiene-Epidemiology-Vector Surveillance and Control of the Matanzas University of Medical Sciences. Materials and methods: retrospective, cross-sectional, descriptive research inserted into the Branch Program of Chronic Non-communicable Diseases. It dealt with the universe of 50 students of the studies of Hygiene-Epidemiology-Vectorial Surveillance and Control of the Matanzas University of Medical Sciences. Results: in relation to the age of beginning to have sexual relationships, it was observed the predominance of the age of 14 years. The presence of multiple sexual partners, unprotected intercourse, use of contraceptive tablets, smoking habit, gynecological manipulations, and sexually transmitted infections are other risk factors found. Special significance had the existence of multiple sexual partners and the use of contraceptive tables. Among the motivations leading to early beginning to have sexual relationships, experiencing new sensations with 28 % and seeking for pleasure with the couple with 55 % stood out. 92 % did not know the disease's risk factors. Conclusions: the students presented pronounced magnitude risk factors of suffering cervical cancer (AU).


Subject(s)
Humans , Adolescent , Young Adult , Uterine Neoplasms/epidemiology , Health Knowledge, Attitudes, Practice , Chronic Disease , Risk Factors , Uterine Neoplasms/prevention & control , Epidemiology, Descriptive , Retrospective Studies , Vector Control , Epidemiologic Surveillance Services
12.
Article in Chinese | WPRIM | ID: wpr-910317

ABSTRACT

Objective:To compare the dosimetric difference between IMRT and VMAT plans for ovarian protection after cervical cancer ovarian transposition surgery.Methods:Thirty-one patients who had received both cervical cancer resection and ovarian transposition were selected for adjuvant radiotherapy. The 9-field evenly divided IMRT and the dual-arc VMAT technology were performed for the treatment planning. The difference of the ovarian mean dose between the two techniques was explored. The relationship between the position of the ovarian-target interval and the ovarian dose was also analyzed.Results:A total of 54 ovaries in 31 patients were effectively transposed and moved out of the target area. Among them, 9 ovaries were located above the upper boundary of the PTV. For these cases, the ovarian mean dose of IMRT and VMAT were (177.8±90.7) and (166.7±70.6) cGy, respectively, which was not statistically different( P>0.05).45 ovaries were located in the same level with PTV. For these cases, the ovarian mean dose of IMRT and VMAT were (459.1±239.9) and (428.3±238.2) cGy, respectively ( z=3.11, P=0.002). The ovarian mean dose has the highest correlation and negative correlation with the closest lateral distance from the ovarian volume center to the PTV surface (IMRT, r=-0.922, P=0.001; VMAT, r=-0.865, P=0.001). To reduce the ovarian mean dose to 500 cGy, the lateral closest distance between the ovarian volume center and the PTV surface should be 3.6 cm and 3.3 cm for IMRT and VMAT respectively. Conclusions:There is no difference between the two planned ovarian doses when the ovaries were located above the upper boundary of the PTV. When the ovaries were located in the same level with PTV, the VMAT plan is better than IMRT in both ovarian dose and treatment efficiency. The ovarian dose could be predicted by the lateral closest distance from the ovarian volume center to the PTV.

13.
Article in Chinese | WPRIM | ID: wpr-910316

ABSTRACT

Objective:To design a knowledge-based cervical cancer planning model and apply it to cases of endometrial cancer and rectal cancer in order to explore the generalization of the model.Methods:A total of 179 cases of pelvic regions with different prescribed doses of dual-arc volumetric modulated arc therapy clinical plans were collected, of which 99 cases of cervical cancer clinical plans with a prescribed dose of 50.4 Gy were used as the training set to establish the RapidPlan model, and the remaining clinical plans were divided into 4 validation groups with 20 cases in each group. The clinical plans for cervical cancer and endometrial cancer with a prescription dose of 50.4 Gy were named groups A and B, while the clinical plan for endometrial cancer and rectal cancer with a prescription dose of 45 Gy were named groups C and D. The model was used to redesign the clinical plans in the 4 groups and the automatic plans were obtained. The planning target volume (PTV) and organ at risk (OAR) dosimetry parameters were compared between automatic plans and clinical plans.Results:The conformity index (CI) of the automatic plans in the A, B, C, and D groups were equivalent to that of the clinical plans ( P>0.05). The homogeneity index (HI) and D2% of the automatic plans in groups A, B, and C were all lower than those in clinical plans(HI, Z=-3.248, -3.360, -2.329, P<0.05; D2%, Z=-2.987, -3.397, -2.442, P<0.05). The HI and D2% of the automatic plans in group D were similar those in the clinical plans ( P>0.05). While ensuring the PTV coverage, the average value of OAR dosimetry parameters in all automatic plans groups were lower than that of the clinical plans. Conclusions:The RapidPlan model established by the cervical cancer clinical plans can complete the automatic plan design for endometrial cancer and rectal cancer under different prescription doses, which preliminarily proves the possibility of the generalization of the RapidPlan model.

14.
Article in Chinese | WPRIM | ID: wpr-907820

ABSTRACT

Objective:To explore the effects of long non-coding RNA SATB2-AS1 in the proliferation and apoptosis of cervical cancer cells as an endogenous competitive RNA to regulate miR-373-5p/BTG3 axis.Methods:qRT-PCR was used to detect the expression of LncRNA SATB2-AS1, miR-373-5p and BTG3 in paracancerous tissue and cancerous tissue of patients with CC. The interaction between LncRNA SATB2/miR-373-5p/BTG3 was then predicted and verified. The expression of SATB2-AS1 and miR-373-5p in cells was intervened, subsequently the CC cells were divided into different groups. The proliferation activity of cells in each group was detected by MTT, and the apoptosis of cells in each group was detected by flow cytometry.Results:qRT-PCR showed that the expression of SATB2-AS1 and BTG3 in cancer tissue and CC cells was significantly decreased, while the expression of miR-373-5p was significantly increased in cancer tissue and CC cells compared with paracancerous tissue and normal cervical cells.The targeting relationship between SATB2-AS1 and miR-373-5p was confirmed. Compared with NC group, overexpression of SATB2-AS1 inhibited cell proliferation butinduced apoptosis. Overexpression of miR-373-5p promoted cell proliferation, inhibited apoptosis, but this effect was partially saved by SATB2-AS1.Conclusion:Up-regulation of LncRNA SATB2-AS1 expression regulated the miR-373-5p/BTG3 axis and participated in the progression of cervical cancer, subsequently inhibited cancer cell proliferation but induced apoptosis.

15.
Article in Chinese | WPRIM | ID: wpr-907788

ABSTRACT

Objective:To investigate the effect of low expression of human epidermal growth factor-like domain protein 7 (EGFL7) gene in cervical cancer cell Hela on its migration and invasion ability.Methods:Cells in the experimental group used small interfering RNA (siRNA) to target the human EGFL7 gene to reduce the expression of EGFL7 in human cervical cancer cells Hela, and cells in the control group were transfected with Mock-siRNA. Real-time quantitative PCR was used to detect the EGFL7 mRNA content of cancer cells in each group; Western blot was used to detect EGFL7 protein expression of cancer cells in each group; The cell scratch healing experiment and Transwell experiment were used to detect the migration and invasion ability of Hela cells in each group.Results:siRNA reduced the protein expression of EGFL7 in human cervical cancer cell Hela. The wound closure percentage of Hela cells in the control group was 74.1%±6.8%. After the expression of EGFL7 was reduced, the percentage of cervical cancer cells was 42%±4.9%, and the wound closure ability was significantly reduced ( P<0.05) . The results of Transwell cell transfer showed that the number of cells successfully transferred by Hela cells in the control group was 179.24±20.01, while the number of cells successfully transferred by Hela cells with low EGFL7 expression was 79.22±13.16. The transfer ability of cells transfected with EGFL7-siRNA was significantly reduced ( P<0.05) . The results of invasion experiments showed that the number of successfully transferred cells in the control group was 79.35±8.04, the number of cells successfully transferred in the EGFL7-siRNA group was 26.98±6.24, and the invasion ability of Hela cells with low expression of EGFL7 decreased ( P< 0.05) . The expression of E-cad in Hela cells with low expression of EGFL7 was up-regulated, and the expression of MMP2/9 protein was down-regulated (all P<0.05) . Conclusion:The low expression of EGFL7 can reduce the migration and invasion ability of cervical cancer cell Hela through the EMT pathway.

16.
Article in Chinese | WPRIM | ID: wpr-907138

ABSTRACT

In May 2018, the World Health Organization (WHO) called for the "global elimination of cervical cancer" and China committed to join 194 countries worldwide to "eliminate cervical cancer". In November 2020, WHO released the Global Strategy for Accelerating the Elimination of Cervical Cancer, specifying the 2030 strategic goal. China has a large population, which makes the task of eliminating cervical cancer difficult. Based on the global and local burden of cervical cancer,we discussed the current situation of cervical cancer in China in the achievement of the elimination. We showed that in order to achieve the phased elimination goal of cervical cancer by 2030, the supply of HPV vaccine needs to increase by 38% every year, the HPV vaccination coverage needs to increase by 45% every year, and the screening coverage of cervical cancer in appropriate-aged women needs to increase by 10% every year. In conclusion, efforts should be made to expand cervical cancer screening, and to incorporate HPV vaccination into the national immunization program.

17.
Article in Chinese | WPRIM | ID: wpr-907115

ABSTRACT

In May 2018, the World Health Organization (WHO) called for the "global elimination of cervical cancer" and China committed to join 194 countries worldwide to "eliminate cervical cancer". In November 2020, WHO released the Global Strategy for Accelerating the Elimination of Cervical Cancer, specifying the 2030 strategic goal. China has a large population, which makes the task of eliminating cervical cancer difficult. Based on the global and local burden of cervical cancer,we discussed the current situation of cervical cancer in China in the achievement of the elimination. We showed that in order to achieve the phased elimination goal of cervical cancer by 2030, the supply of HPV vaccine needs to increase by 38% every year, the HPV vaccination coverage needs to increase by 45% every year, and the screening coverage of cervical cancer in appropriate-aged women needs to increase by 10% every year. In conclusion, efforts should be made to expand cervical cancer screening, and to incorporate HPV vaccination into the national immunization program.

18.
China Pharmacy ; (12): 2827-2831, 2021.
Article in Chinese | WPRIM | ID: wpr-906647

ABSTRACT

OBJECTIVE:To study the effects and mechanism of oncolytic virus M 1(called M 1 virus for short )inducing the apoptosis of cervical cancer C-33A cells. METHODS :MTT assay was used to detect survival rate of C- 33A cells that were treated with different titers (0,0.001,0.01,0.1,1,10 PFU/cell)of M 1 virus. C- 33A cells were divided into control group (0 PFU/cell), low-dose,medium-dose and high-dose groups of M 1 virus(0.001,0.01,0.1 PFU/cell). After treated with corresponding titers of M1 virus for 48 h,flow cytometry was used to detect the apoptotic rate and infection rate of cells;Western blot was performed to detect the protein expression of C/EBP homologous proteins (CHOP),caspase-12,caspase-3 and cleaved-caspase- 3. RESULTS : After treated with different titers of M 1 virus,the survival rate of C- 33A cells decreased significantly (P<0.01),and showed a dose-dependent tr end. Compared with control group ,the apoptotic rate and infection rate of cells in M 1 virus groups as well as the protein expression of CHOP ,caspase-12 and cleaved-caspase- 3(except for medium-dose group )in M 1 virus medium-dose and high-dose groups were increased significantly (P<0.01). CONCLUSIONS :M1 virus can induce the apoptosis of cervical cancer C-33A cells ,and its mechanism may be related to the activation of endoplasmic reticulum stress pathway.

19.
Lao Medical Journal ; : 14-21, 2021.
Article in English | WPRIM | ID: wpr-904536

ABSTRACT

@#Objective: we aimed to assess the knowledge, attitude and practice (KAP) of healthcare providers (HCP) towards cervical cancer (CC) screening and to identify possible factors associated with its low utilization among women presenting at gynaeco-obstetrics units in the Lao PDR. Methods: A cross-sectional study was conducted between March - June 2018 on a sample of 85 (HCP) at gynaecology units in two provincial and eleven district hospitals in Luang Prabang (LPB) and Salavan (SLV) Provinces. Results: Of the 85 HCP, 63.4% were from SLV and 36.6% from LPB. 81% were females and mean ager was 32 years. Only 7% of them had good knowledge, 18.8% had good CC screening attitudes and only 1.2% had good CC screening practices. 36.2% of female HCP had been screened for CC and 48.3% had not been screened because they thought only those who had symptoms and risk factors should go for. The most common reasons for not conducting routine CC screening of patients were: lack of medical equipment (53.7%), and incompetent techniques (43.3%). HCP graduates and post graduates had a higher knowledge score (aOR = 4.09, 95% CI: 1.43-11.66, P = 0.008), and attitude score (aOR = 5.54, 95% CI: 1.55-19.75, p=0.008). Those, who had been working for more than 10 years, were more likely to have higher attitude scores (aOR = 6.07, 95% CI: 1.36-27.15, p =0.018). Conclusions: CC screening knowledge among HCP is generally fair. However, CC screening attitudes are still poor. Re-orientation courses on CC screening for HCP are urgently needed in order to move forward to the next steps in CC screening programs.

20.
Article in English | WPRIM | ID: wpr-922764

ABSTRACT

Cervical cancer (CC) is recognized as the most common neoplasm in the female reproductive system worldwide. The lack of chemotherapeutic agents with outstanding effectiveness and safety severely compromises the anti-cipated prognosis of patients. Aloperine (ALO) is a natural quinolizidine alkaloid with marked anti-cancer effects on multiple malignancies as well as favorable activity in relieving inflammation, allergies and infection. However, its therapeutic efficacy and underlying mechanism in CC are still unclear. In the current study, MTT assay was employed to evaluate the viability of HeLa cells exposed to ALO to preliminarily estimate the effectiveness of ALO in CC. Then, the effects of ALO on the proliferation and apoptosis of HeLa cells were further investigated by plate colony formation and flow cytometry, respectively, while the migration and invasion of ALO-treated HeLa cells were evaluated using Transwell assay. Moreover, nude mice were subcutaneously inoculated with HeLa cells to demonstrate the anti-CC properties of ALO in vivo. The molecular mechanisms underlying these effects of ALO were evaluated by Western blot and immunohistochemical analysis. This study experimentally demonstrated that ALO inhibited the proliferation of HeLa cells via G2 phase cell cycle arrest. Simultaneously, ALO promoted an increase in the percentage of apoptotic HeLa cells by increasing the Bax/Bcl-2 ratio. Additionally, the migration and invasion of HeLa cells were attenuated by ALO treatment, which was considered to result from inhibition of epithelial-to-mesenchymal transition. For molecular mechanisms, the expression and activation of the IL-6-JAK1-STAT3 feedback loop were markedly suppressed by ALO treatment. This study indicated that ALO markedly suppresses the proliferation, migration and invasion and enhances the apoptosis of HeLa cells. In addition, these prominent anti-CC properties of ALO are associated with repression of the IL-6-JAK1-STAT3 feedback loop.


Subject(s)
Animals , Apoptosis , Cell Line, Tumor , Cell Movement , Cell Proliferation , Feedback , Female , HeLa Cells , Humans , Interleukin-6/genetics , Janus Kinase 1 , Mice , Mice, Nude , Quinolizidines , STAT3 Transcription Factor/genetics , Signal Transduction , Uterine Cervical Neoplasms/drug therapy
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