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1.
Braz. j. biol ; 82: e254291, 2022. tab, graf
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1355847

ABSTRACT

Abstract Medicinal plants have long been prescribed in Thailand for centuries. Different constituents of extracts have been used for treating of various infectious diseases. However, there is even less information available regarding the use in fungal skin infection. In order to assess traditional Thai claims about the therapeutic potential, this study is focused on exploring the anti-dermatophyte property of the plants that are currently used as traditional medicines. The potential of four different plant species were selected for investigate in vitro anti-dermatophyte activity. Ethanolic extracts of Chromolaena odorata (L.), Ageratina adenophora (Spreng.), Eclipta prostrate (Linn.), and Acorus calamus (L.). were analysed for their total phenolic content as well as total flavonoid content and were then subjected to test of their anti-dermatophyte properties using agar well diffusion method. Qualitative flavonoids and phenolics analysis of the extracts showed their biologically active constituents. Among the species examined, the result indicated that most of the extracts demonstrated anti-dermatophyte activity. In particular, A. calamus showed the highest efficacy against test organisms. The experiment confirmed the chemical constituents and efficacy of some selected plants and provides a scientific confirmation of the use of Thai plants in traditional medicine for fungal skin infections.


Resumo As plantas medicinais são prescritas há muito tempo na Tailândia, há séculos. Diferentes constituintes de extratos têm sido usados para o tratamento de várias doenças infecciosas. No entanto, existem ainda menos informações disponíveis sobre o uso em infecções fúngicas da pele. A fim de avaliar as alegações tradicionais tailandesas sobre o potencial terapêutico, este estudo está focado em explorar a propriedade antidermatófita das plantas que são usadas atualmente como medicamentos tradicionais. O potencial de quatro espécies de plantas diferentes foi selecionado para investigar a atividade antidermatófita in vitro. Extratos etanólicos de Chromolaena odorata (L.), Ageratina adenophora (Spreng.), Eclipta prostrate (Linn.) e Acorus calamus (L.) foram analisados quanto ao seu conteúdo fenólico total, bem como ao conteúdo de flavonoides totais. E então submetidos ao teste de suas propriedades antidermatófitas usando o método de difusão em ágar bem. A análise qualitativa de flavonoides e fenólicos dos extratos mostrou seus constituintes biologicamente ativos. Entre as espécies examinadas, o resultado indicou que a maioria dos extratos demonstrou atividade antidermatófita. Em particular, A. calamus mostrou a maior eficácia contra organismos de teste. O experimento confirmou os constituintes químicos e a eficácia de algumas plantas selecionadas e fornece uma confirmação científica do uso de plantas tailandesas na medicina tradicional para infecções fúngicas da pele.


Subject(s)
Plants, Medicinal , Phenols/analysis , Thailand , Plant Extracts/pharmacology , Medicine, Traditional
2.
Rev. Ciênc. Méd. Biol. (Impr.) ; 20(1): 120-124, maio 5, 2021. fig, tab
Article in Portuguese | LILACS | ID: biblio-1355053

ABSTRACT

Introduction: dermatophytoses or "tineas" are characterized by being mycoses caused by fungi of the genera Epidermophyton, Trichophyton and Microsporum. These mycotic infections can present themselves as a form of lesions that affect the skin, hair and nails of individuals of both genders and all ages. Objective: to elucidate the epidemiological profile of dermatophytoses in patients examined by a private clinical analysis laboratory in João Pessoa-PB, between 2015 and 2019. Methodology: this is an epidemiological, analytical, retrospective and documentary study, in which data collection took place at the Clinical Pathology Laboratory ­ "HEMATO", located in João Pessoa ­ PB. Results: the profile of those affected was predominantly female (58.5%), 18 to 59 years old (38.4%), white (53.6%) and with lesions, mainly in skin glabrous (38.5%), feet (33.3%) and nails (12.8%). When relating the age group to the injury site, it was noticed that injuries on glabrous skin, feet and nails, were more frequent in individuals aged 18 to 59 years, while injuries to the scalp were mostly found in individuals younger than 18 years old. The most prevalent species were M. canis (31.9%) and T.rubrum (31.9%). When correlating the fungal species with the lesion site, it was noted that M. canis was the main agent responsible for lesions in glabrous skin, scalp and hands, while T. rubrum was predominantly observed in nails and T. mentagrophytes in feet. Conclusion: it is concluded that the data present in this research can promote the development of indicators and public policies for the population most susceptible to dermatophytosis.


Introdução: dermatofitoses ou tineas se caracterizam por serem micoses causadas por fungos dos gêneros Epidermophyton, Trichophyton e Microsporum. Essas infecções micóticas podem se apresentar na forma de lesões que acometem pele, pelo e unhas de indivíduos de ambos os gêneros e todas as idades. Objetivo: elucidar o perfil epidemiológico de dermatofitoses de pacientes atendidos por um laboratório privado de análises clínicas em João Pessoa-PB, entre 2015 a 2019. Metodologia: trata-se de um estudo epidemiológico, analítico, retrospectivo e documental, em que a coleta de dados ocorreu no Laboratório de Patologia Clínica ­ HEMATO, localizado em João Pessoa ­ PB. Resultados: o perfil de acometidos foi predominantemente de indivíduos do sexo feminino (58,5%), com 18 a 59 anos de idade (38,4%), brancos (53,6%) e com lesões, principalmente, em pele glabra (38,5%), pés (33,3%) e unhas (12,8%). Ao relacionar a faixa etária com o local da lesão, percebeu-se que lesões em pele glabra, pés e unhas, foram mais frequentes em indivíduos de 18 a 59 anos, enquanto que lesões no couro cabeludo foram majoritariamente encontradas em indivíduos menos de 18 anos. As espécies mais prevalentes foram M. canis (31,9%) e T. rubrum (31,9%). Ao correlacionar a espécie fúngica com o local da lesão, notou-se que M. canis foi o principal agente responsável por lesões em pele glabra, couro cabeludo e mãos, enquanto T. rubrum foi predominantemente observado em unhas e T. mentagrophytes em pés. Conclusão: os dados obtidos nesta pesquisa podem fomentar o desenvolvimento de indicadores e políticas públicas para a população mais susceptível às dermatofitoses.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Aged , Tinea , Arthrodermataceae , Fungi , Analytical Methods , Epidemiologic Methods , Retrospective Studies
3.
Rev. chil. pediatr ; 91(1): 131-138, feb. 2020. tab, graf
Article in Spanish | LILACS | ID: biblio-1092798

ABSTRACT

Resumen: La onicomicosis (OM) es una infección fúngica de las uñas, cuyo principal agente causal es el Tricophytum rubrum. Si bien es una patología infrecuente en niños, se ha observado un aumento en la prevalencia en el último tiempo. Hasta la fecha, existen diversos estudios y guías clínicas de OM en adultos. Sin embargo, la literatura en edad pediátrica es escasa, lo que dificulta el tratamiento en pediatría. En el presente articulo se revisa la literatura actual, los métodos diagnosticos de OM, datos epidemiológicos locales y globales, y se presentan las opciones de tratamiento disponibles conside rando su eficacia y perfil de seguridad en población pediátrica.


Abstract: Onychomycosis (OM) is a fungal infection of the nails, whose main etiologic agent is Trichophytum rubrum. Although, it is an unusual pathology in children, in the last years an increase in its preva lence has been observed. To date, there are several studies and clinical guidelines for OM in adults. However, literature in children is scarce, which makes pediatric treatment difficult. The objective of this publication was to review the current literature in order to establish diagnostic methods for OM, national and international epidemiological data, and to provide treatment options taking into account their efficiency and safety profile in the pediatric population.


Subject(s)
Humans , Infant , Child, Preschool , Child , Adolescent , Onychomycosis/diagnosis , Onychomycosis/microbiology , Onychomycosis/drug therapy , Onychomycosis/epidemiology , Pediatrics , Global Health , Antifungal Agents/therapeutic use
4.
Rev. Nac. (Itauguá) ; 11(2): 30-40, DICIEMBRE 2019.
Article in Spanish | LILACS-Express | LILACS, BDNPAR | ID: biblio-1046299

ABSTRACT

Introducción: las micosis superficiales o dermatomicosis se encuentran entre las enfermedades infecciosas de piel más comunes. Se localizan en las capas superficiales de la piel y sus anexos (pelo y uñas), por esta razón son motivo de consulta dermatológica. De estas micosis, las dermatofitosis, las malasseziosis y las candidiasis cutáneas son las más frecuentes. Objetivo: describir las características epidemiológicas de las micosis superficiales de piel lampiña. Metodología: estudio observacional, retrospectivo y de corte transverso, donde se analizaron fichas epidemiológicas de pacientes que acudieron al Centro de Especialidades Dermatológicas en el periodo comprendido entre julio del 2017 hasta agosto del 2019, con diagnóstico presuntivo de micosis superficiales de piel lampiña. Resultados: se observaron 341 casos de micosis superficiales de piel lampiña. Entre los agentes causales, los dermatofitos fueron los responsables del 56 % (190/341) de los casos; Malassezia spp. 42 % (142/341) y Candida spp. 2 % 9/341. Se observaron 3 casos de micosis mixtas, donde Candida spp. y dermatofitos fueron los agentes causales. Conclusión: entre las micosis superficiales que afectan piel lampiña, se encuentran en primer lugar los dermatofitos seguidos por las malasseziosis. Este reporte constituye un aporte importante para el conocimiento de la epidemiología de las micosis superficiales en nuestro país.


Introduction: superficial mycoses or dermatomycosis are among the most common skin infectious diseases. These are located in the superficial layers of the skin and its annexes (hair and nails), for this reason it´s a frecuent cause of medical consult. Of these mycoses, dermatophytosis, malasseziosis and cutaneous candidiasis are the most frequent. Objectives: describe the epidemiological characteristics of superficial mycoses of skin. Methodology: observational, retrospective and cross-sectional study, where epidemiological records of patients who were attended at the Dermatological Specialties Center were analyzed in the period from July 2017 to August 2019, with presumptive diagnosis of superficial mycoses of skin. Results: 341 cases of superficial mycosis of skin were observed. Among the causative agents, dermatophytes were responsible for 56 % (190/341) of the cases; Malassezia sp. 42 % (142/341) and Candida sp. 2 % (9/341). Three cases of mixed mycosis were observed; Candida spp. and Dermatophytes were the causative agents. Conclusion: among the superficial mycoses of skin, dermatophytes followed by malaseziosis are in the first places. This report constitutes an important contribution to the knowledge of the epidemiology of superficial mycoses in our country.

5.
Rev. Nac. (Itauguá) ; 11(2): 19-29, DICIEMBRE 2019.
Article in Spanish | LILACS-Express | LILACS, BDNPAR | ID: biblio-1046298

ABSTRACT

Introducción: la tiña de la cabeza es una micosis superficial producida por dermatofitos, más común en niños y excepcional en los adultos. Objetivo: conocer las características epidemiológicas y micológicas de la tiña de la cabeza en pacientes que acudieron al Centro de Especialidades Dermatológicas en la ciudad de San Lorenzo. Metodología: estudio observacional retrospectivo de corte transverso, donde se analizaron fichas y registros de todos los pacientes con sospecha clínica de tiña de la cabeza remitidos al laboratorio del Centro de Especialidades Dermatológicas en el periodo julio 2017 - agosto 2019. Los pacientes fueron sometidos a estudios micológicos directos y cultivos para la confirmación del diagnóstico e identificación del agente etiológico. Se consideró el sexo, edad, residencia, posesión de mascotas domésticas y agente etiológico aislado. Resultados: se estudiaron 132 pacientes con diagnóstico de tiña de la cabeza, 69 (52.3 %) varones y 63 (47.7 %) mujeres. La edad de presentación fue desde 1 año hasta 14 años, con una media de 5 años. El principal agente etiológico aislado fue Microsporum canis 99/132 (75 %) seguido de Trichophyton tonsurans 29/132 (22 %). Conclusiones: los hallazgos epidemiológicos obtenidos coinciden con otros trabajos científicos, destacándose M. canis y T. tonsurans como los principales agentes etiológicos.


Introduction: tinea capitis is a superficial mycosis produced by dermatophytes, more common in children and exceptional in adults. Objectives: to know the epidemiological and mycological characteristics of tinea capitis in patients who were attended in the Dermatological Specialties Center of San Lorenzo. Methodology: retrospective, observational and cross-sectional study, in which the files and records of all patients with clinical suspicion of tinea capitis, referred to the laboratory of the Dermatological Specialties Center in the period july 2017 - august 2019 were analyzed. The patients underwent direct mycological studies and cultures for confirmation of the diagnosis and identification of the etiologic agent. The sex, age, residence, possession of domestic pets and isolated etiological agent were considered. Results: 132 patients with a diagnosis of tinea capitis, 69 (52.3 %) men and 63 (47.7 %) women were studied. The age of presentation was from 1 year to 14 years, with an average of 5 years. The main etiological agent isolated was Microsporum canis 99/132 (75 %) followed by Trichophyton tonsurans 29/132 (22 %). Conclusions: the epidemiological findings obtained are agree with other scientific studies, highlighting M. canis and T. tonsurans as the main etiological agents.

6.
Rev. bras. anal. clin ; 51(3): 202-207, 20190930. graf, tab
Article in Portuguese | LILACS | ID: biblio-1047635

ABSTRACT

Objetivo: As infecções fúngicas, atualmente, constituem um problema de saúde pública, devido à elevada prevalência e frequência com que acometem a população. Fatores predisponentes como condições climáticas, região geográfica e características culturais e socioeconômicas favorecem a ocorrência destas infecções. O objetivo do presente estudo foi determinar a prevalência das infecções fúngicas em pacientes atendidos em um laboratório de análises clínicas na cidade de Veranópolis, Rio Grande do Sul (RS). Métodos: Foi realizado um estudo retrospectivo, por meio da consulta ao banco de dados, de 403 laudos de exames micológicos, direto e cultural, realizados no laboratório durante o período de abril de 2014 a abril de 2017. Resultados: Dentre os laudos analisados no estudo, houve positividade em 251 (62,28%) exames micológicos diretos (EMD) e em 226 (56,08%) exames culturais. As micoses superficiais e cutâneas constituíram as principais infecções diagnosticadas, sendo os fungos dermatófitos os agentes etiológicos mais isolados (69,47%), representados majoritariamente pela espécie Trichophyton mentagrophytes, seguidos por leveduras (20,35%), especialmente as pertencentes ao gênero Candida. Houve predomínio de infecções em pacientes do sexo feminino (64,54% EMD e 61,5% cultura) e adultos de 31 a 60 anos foram os mais acometidos pelas micoses diagnosticadas (58,96% EMD e 60,62% cultura). Conclusão: O conhecimento do perfil epidemiológico pode servir como guia na correta identificação do patógeno no diagnóstico laboratorial, influenciando diretamente no prognóstico e na escolha da conduta terapêutica adequada.


Objective: The fungal infections are a public health issue, because ofthe high prevalence and frequency that they victimize the population. Predisposal factors as bioclimatic conditions, geographic region and cultural and socioeconomic characteristics favor the occurrence of this infections, generating, this way, variation in the prevalence of species in each region. The goal of the present study was to determinate the prevalence of fungal infections in patients in a clinical analysis laboratory in the city of Veranópolis, Rio Grande do Sul (RS). Methods: A retrospective study was made, using the consult of data basis, of 403 direct and culture mycological examination reports made in the laboratory during the period of April 2014 to April 2017. Results: In the analyzed examination reports in the study, there was positivity in 251 (62,28%) mycological direct exams (EMD) and, in 226 (56,08%) cultural exams. The superficial and cutaneous mycosis constituted the mainly diagnosed infections, being the dermatophytic fungi the most isolated etiological factors (69,47%), represented mainly, by the Trichophyton mentagrophytes species, followed by yeasts (20,35%), especially those who belong to the Candida genre. There was prevalence of infections in women patients (64,54% EMD and 61,5% culture). Adults among 31 and 60 years old were the most victimized by the diagnosed mycosis (58,96% EMD e 60,62% cultural). Conclusion: Knowledge of the epidemiological profile can serve as guide in the correct identification of the pathogen in the laboratory diagnosis, influencing directly in the prognosis and in the choice of the right therapeutic conduct


Subject(s)
Humans , Male , Female , Infant, Newborn , Infant , Child, Preschool , Child , Adolescent , Adult , Candida , Epidemiology , Dermatomycoses , Arthrodermataceae , Fungi
7.
Arq. bras. med. vet. zootec. (Online) ; 71(4): 1143-1148, jul.-ago. 2019. tab, ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1038631

ABSTRACT

Objetivou-se neste estudo padronizar um protocolo de reação em cadeia da polimerase (PCR) para detecção de Microsporum canis em amostras de pelos e/ou crostas de cães e gatos. Foram selecionadas 48 amostras previamente identificadas por meio de cultura. Destas, 23 foram positivas para dermatófitos no cultivo. Padronizou-se a PCR a partir de primers desenhados para o alvo M. canis. Sessenta e um por cento (14/23) das amostras positivas para dermatófitos foram identificadas como M. canis em cultura. Desse total, 71,4% (10/14) apresentaram um fragmento de 218pb compatível com o esperado para a espécie fúngica alvo dessa reação. Observou-se uma sensibilidade de 71,4% e especificidade de 100% na PCR, além de uma boa concordância entre essas técnicas de diagnóstico (Kappa: 0,78; P<0,0001). O protocolo utilizado neste estudo apresentou alta especificidade na detecção de M. canis diretamente de amostras de pelos e/ou crostas de cães e gatos, viabilizando um diagnóstico mais rápido e específico, podendo esse protocolo ser empregado como um método confirmatório para agilizar a detecção de M. canis.(AU)


The aim of this study was to standardize a Polymerase Chain Reaction protocol (PCR) for the detection of Microsporum canis in fur and/or crusts of dogs and cats. 48 samples previously identified by culture were selected. Of these, 23 were positive for dermatophytes in culture. PCR was standardized from drawn primers whose target is M. canis. A total of 61% (14/23) of the dermatophyte positive samples were identified as M. canis in culture. Of this total, 71.4% (10/14) presented a fragment of 218bp compatible with that expected for the fungal species target of the reaction. A sensitivity of 71.4% and specificity of 100% in the PCR were observed, in addition to a good agreement between the techniques (Kappa: 0.78; P<0.0001). The protocol used in this study showed high specificity in the detection of M. canis directly from fur and/or crusts of dogs and cats, making possible a faster and more specific diagnosis. This protocol could be used as a confirmatory method, speeding the detection of M. canis.(AU)


Subject(s)
Animals , Cats , Dogs , Polymerase Chain Reaction/methods , Polymerase Chain Reaction/veterinary , Dermatomycoses/diagnosis , Dermatomycoses/veterinary , Animal Fur/microbiology , Microsporum , Molecular Diagnostic Techniques/veterinary
8.
Pesqui. vet. bras ; 38(9): 1824-1828, set. 2018. tab, graf
Article in Portuguese | LILACS, VETINDEX | ID: biblio-976504

ABSTRACT

Objetivou-se padronizar uma reação do tipo multiplex PCR (mPCR) para detectar Microsporum canis, Microsporum gypseum e o complexo Trichophyton mentagrophytes em amostras de pelos e/ou crostas de cães e gatos. 250 amostras de pelos e/ou crostas de cães e gatos foram analisadas por meio de exame direto e cultura, o DNA das mesmas foi extraído para mPCR. Primers foram desenhados e como controle positivo da reação utilizou-se o DNA extraído de colônias de M. canis (URM 6273), M. gypseum (URM 6921) e T. mentagrophytes (URM 6211), provenientes da Coleção de Culturas (Micoteca URM), Departamento de Micologia, Centro de Ciências Biológicas da Universidade Federal de Pernambuco (CCB/UFPE). Como controles negativos de reação, utilizou-se água destilada esterilizada e DNA extraído de Alternaria sp. para verificar a especificidade dos primers. Do total de amostras analisadas, 15 (6%) foram identificadas, em cultura, como dermatófitos, e destas, 10 foram M. canis, três M. gypseum e dois T. mentagrophytes (complexo). Destas 15 amostras positivas, 11 (73,3%) foram detectadas por meio da mPCR. Além destas, seis outras, negativas em cultura, foram identificadas como M. gypseum. Verificou-se uma boa concordância entre os resultados da cultura e mPCR (Kappa: 0,66). O protocolo padronizado neste estudo pode ser utilizado como um método de triagem, por apresentar uma sensibilidade maior que a da cultura, usado paralelamente aos exames de rotina, permitindo um diagnóstico em menor tempo.(AU)


The aim of this study was to standardize a multiplex PCR (mPCR) reaction to detect Microsporum canis, Microsporum gypseum and the Trichophyton mentagrophytes complex in dog and cat fur and/or crusts. 250 fur and/or crusts samples from dogs and cats were analyzed by direct examination and culture, DNA from them was extracted for mPCR. Primers were designed and the DNA extracted from colonies of M. canis (URM 6273), M. gypseum (URM 6921) and T. mentagrophytes (URM 6211) from the Collection of Cultures - URM Micoteca - Department of Mycology, Biological Sciences Center of the Federal University of Pernambuco (CCB / UFPE). As negative controls, sterile distilled water and DNA extracted from Alternaria sp., were used to verify the specificity of the primers. Of the total samples analyzed, 15 (6%) were identified in culture as dermatophytes, and of these, 10 were M. canis, three M. gypseum and two T. mentagrophytes (complex). Of these 15 positive samples, 11 (73.3%) were detected by mPCR. Besides these, six others, negative in culture, were identified as M. gypseum. There was good agreement between culture results and mPCR (Kappa: 0.66). The protocol standardized in this study can be used as a screening method, because it has a sensitivity greater than that of the culture, used in parallel to the routine exams, allowing a diagnosis in a shorter time.(AU)


Subject(s)
Animals , Cats , Dogs , Arthrodermataceae , Multiplex Polymerase Chain Reaction/statistics & numerical data , Keratins , Microsporum/classification
9.
Rev. Ciênc. Méd. Biol. (Impr.) ; 17(2): 190-193, maio -jun. 2018. ilus
Article in Portuguese | LILACS | ID: biblio-1224415

ABSTRACT

Introdução: as dermatofitoses são micoses causadas por fungos, denominados dermatófitos, que produzem lesões na pele, pelos ou unhas. Nas academias, o risco de contaminação é maior pois trata-se de um ambiente fechado com grande fluxo de pessoas, constante uso dos mesmos equipamentos, além do suor proveniente de atividade física e limpeza inadequada que influenciam na proliferação desses fungos. Objetivos: a realização desta pesquisa teve como objetivo identificar a presença de dermatófitos nos tatames e sacos de pancada das academias de artes marciais localizadas na região central, norte e sul do município de Palmas ­ TO. Metodologia: utilizou-se a técnica de "swab umedecido" para a coleta de 18 amostras em três academias de artes marciais, posteriormente semeadas e isoladas em duplicata, em meios Sabouraud Dextrose Ágar e Batata Dextrose Ágar para identificação macroscópica das colônias com características filamentosas ou leveduriformes. Posteriormente foram realizadas identificações presuntivas das estruturas microscópicas para análise morfológicas dos fungos dermatófitos de interesse. Resultados: verificou-se que das 36 amostras submetidas à análise, observando-se apenas o gênero Trichophyton sp. em 4,6% das amostras, Zygomicetes sp. em 5,4%, entretanto, o gênero Aspergillus sp. com 89.8% de presença, apresentou-se com maior frequência nesse estudo. Conclusão: de acordo com os resultados do estudo, os produtos de limpeza que são utilizados diariamente, sendo o álcool um dos principais, pode ter contribuído para a inibição do crescimento fúngico de dermatófitos.


Introduction: dermatophytoses are fungal mycoses, called dermatophytes, that produce lesions on the skin, hair or nails. In the academies, the risk of contamination is higher because it is a closed environment with a great flow of people, constant use of the same equipment, besides the sweat coming from physical activity and inadequate cleaning that influence the proliferation of these fungi. Objectives: the objective of this research was to identify the presence of dermatophytes on the tatami mats and punching bags of the martial arts academies located in the central, northern and southern regions of the municipality of Palmas ­ TO. Methodology: the "swab moistened" technique was used to collect 18 samples in three academies of soft arts, later sown and isolated in duplicate, in media Sabouraud Dextrose Agar and Potato Dextrose Agar for macroscopic identification of colonies with filamentous or yeast characteristics. Subsequently, presumptive identifications of the microscopic structures for morphological analysis of the dermatophytic fungi of interest were carried out. Results: it was verified that of the 36 samples submitted to the analysis, observing only the genus Trichophyton sp. in 4,6% of the samples, Zygomicetes sp. in 5,4%, however, the genus Aspergillus sp. with a 89,8% presence, was more frequent in this study. Conclusion: according to the results of the study, cleaning products that are used daily, being the alcohol one of the main ones, may have contributed to the inhibition of the fungal growth of dermatophytes.


Subject(s)
Fungi
10.
Infectio ; 22(2): 105-109, abr.-jun. 2018. tab, graf
Article in English | LILACS, COLNAL | ID: biblio-892761

ABSTRACT

Abstract Introduction: Onychomycosis are fungal nail infections that can be caused by dermatophytes, non-dermatophytic molds and yeasts, which are capable of breaking down keratin. Mixed onychomycosis are a controversial subject and they are the outcome of the combination of two dermatophytes, dermatophytes/nondermatophytic molds or dermatophytes/yeast. Objetives: To determine the frequency of total dystrophic onychomycosis caused by more than one etiological agent (mixed onychomycosis) in outpatients from a Dermatologic Center in Guatemala and to establish the characteristics associated with this fungal infection. Methods: Prospective observational study from August to December of 2012. Nail samples were obtained from patients with total dystrophic onychomycosis to identify the causal agents by culture in Sabouraud dextrose and Mycosel® agar. Results: 32 of 130 patients had mixed onychomycosis. 68.5% were associated to tinea pedis. The most common association was between T. rubrum + Candida, T. rubrum + M. canis and T. rubrum + opportunist fungi. Conclusions: Mixed onychomycosis represent 25% of the total dystrophic onychomycosis in Guatemala. We observed an important relationship between diabetes and the main association was T. rubrum with Candida spp.


Resumen Introducción: Las onicomicosis son infecciones fúngicas de las uñas que pueden ser causadas por dermatofitos, mohos no dermatofitos y levaduras, que son capaces de degradar la queratina. Las onicomicosis mixtas son un tema polémico y es el resultado de la combinación de dos dermatofitos, dermatofitos / mohos no dermatofitos o dermatofitos / levadura. Objetivos: Determinar la frecuencia de la onicomicosis distrófica total causada por más de un agente etiológico (onicomicosis mixta) en pacientes ambulatorios de un Centro Dermatológico en Guatemala y establecer las características asociadas a esta infección fúngica. Métodos: Estudio observacional prospectivo de agosto a diciembre de 2012. Se obtuvieron muestras de uñas de pacientes con onicomicosis distrófica total para identificar los agentes causales en cultivo de agar dextrosa Sabouraud y Mycosel®. Resultados: 32 de 130 pacientes tenían onicomicosis mixta. 68.5% se asociaron a tinea pedis. La asociación más común fue entre T. rubrum + Candida, T. rubrum + M. canis y T. rubrum + hongos oportunistas. Conclusiones: La onicomicosis mixta representa el 25% de la onicomicosis distrófica total en Guatemala. Observamos una relación importante entre la diabetes y la asociación principal fue T. rubrum con Candida spp.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Onychomycosis , Dermatology , Tinea Pedis , Candida , Agar , Arthrodermataceae , Fungi , Guatemala , Infections , Nails
11.
Arq. bras. med. vet. zootec. (Online) ; 70(6): 1747-1753, nov.-dez. 2018. tab
Article in English | LILACS, VETINDEX | ID: biblio-970323

ABSTRACT

The aim of this study was to diagnose dermatophytosis in pets and investigate the presence of dermatophytes in their home environment. Samples from hair coat were collected from 70 pets: 47 dogs, 19 cats, three guinea pigs and one rabbit. After mycological culture, 188 samples were collected from the household environments in 26 homes: 78 from places were of predominantly used by the tutors, 66 from places used by the animals, 44 from flooring, and 24 samples from contactees. Samples were seeded on Mycosel agar, incubated at 25°C, and the colonies were identified by their macro-and-microscopic characteristics. Dermatophytes were found in 37.1% of the samples originating from the sick animals. Microsporum canis was the most prevalent species, isolated in 12 dogs and eight cats; Trichophyton quinckeanum in three guinea pigs, Microsporum gypseum in two dogs and Trichophyton mentagrophytes in one cat. Dermatophytes were found in 69.2% of the surveyed homes; 29.5% of the places/objects predominantly used by the tutors, 42.4% mainly used by the animals, 31.8% from floors, and 50% from contactees. The meeting of dermatophytes in animals and in the household environment confirms the possibility of transmission by direct or indirect contact and their importance in public health.(AU)


O objetivo deste trabalho foi diagnosticar dermatofitose em pets e pesquisar dermatófitos em seu ambiente domiciliar. Colheram-se amostras de 70 pets, 47 cães, 19 gatos, três cobaias e um coelho. Visitaram-se 26 residências dos animais positivos em cultura micológica para a doença, colhendo-se 188 amostras do ambiente: 78 de locais de uso predominante dos tutores, 66 de uso dos animais e 44 de pisos; também foram colhidas 24 amostras de animais contactantes. As amostras clínicas foram semeadas em ágar Mycosel, incubadas a 25°C, e as colônias identificadas por suas características macro e microscópicas. Dermatófitos foram isolados em 37,1% dos animais suspeitos. Microsporum canis foi o mais frequente, sendo isolado de 12 cães e oito gatos, Trichophyton quinckeanum de três cobaias, Microsporum gypseum de dois cães e Trichophyton mentagrophytes de um gato. Foram encontrados dermatófitos em 69,2% das casas pesquisadas, isolando-se esses fungos em 29,5% dos locais/objetos de uso predominante dos tutores, 42,4% de uso predominante dos animais, 31,8% de pisos e 50% dos animais contactantes. O encontro de dermatófitos nos animais e em superfícies inanimadas nas residências confirma a possibilidade de transmissão de dermatofitose por contato direto e indireto e sua importância em saúde pública.(AU)


Subject(s)
Animals , Arthrodermataceae , Pets/microbiology , Zoonoses
12.
Rev. MVZ Córdoba ; 22(3): 6334-6338, Sep.-Dec. 2017. tab
Article in English | LILACS | ID: biblio-1041180

ABSTRACT

ABSTRACT Objective. The dermatophytes are keratinophilic fungi, of importance in public health because of their anthropozoophilic nature. Given the increasing acquisition of exotic animals as pets and the scarce studies on the state of colonization by dermatophytes on these animals; we raised the objective of determine the presence of dermatophytes in clinically healthy rabbits (Oryctolagus cuniculus) from pet stores in Santiago, Chile. Materials and Methods. 42 clinically healthy rabbits were studied. Clinical specimens were obtained from the hairy mantle and cultivated on Sabouraud glucose agar and dermatophyte test medium (DTM™); the identification of the fungal isolates was performed using classic mycological procedures that included direct microscopic examination and the analysis of micromorphological features on culture. Results. Of the total number of rabbits studied, three (7.1%) presented colonization by dermatophytes, being more frequent in males and in those animals that coexisted with Guinea Pigs. The species identified in all three cases was Trichophyton mentagrophytes. Conclusions. This study evidence colonization by dermatophytes in domestic rabbits, important information for veterinarians and owners of pets, at the time of being in contact with this animal species.


RESUMEN Objetivo. Los dermatofitos son hongos queratinofílicos, de importancia en salud pública por su rol antropozoofílico. Dado el aumento en la adquisición de animales exóticos como mascotas y los escasos estudios sobre el estado de colonización por dermatofitos en estos animales, nos planteamos el objetivo de determinar la presencia de dermatofitos en conejos (Oryctolagus cuniculus) clínicamente sanos procedentes de tiendas de mascotas en Santiago de Chile. Materiales y Métodos. Se estudiaron 42 conejos clínicamente sanos. Las muestras clínicas se obtuvieron desde el manto piloso y cultivadas en agar Sabouraud glucosado y dermatophyte test medium (DTM®). La identificación del agente aislado fue realizada por procedimientos micológicos clásicos que incluyeron examen microscópico directo y cultivo. Resultados. Del total de conejos estudiados, tres (7,1%) presentaron colonización por dermatofitos, siendo más frecuente en machos y en aquellos animales que convivían con cuyes. La especie identificada en los tres casos fue Trichophyton mentagrophytes. Conclusiones. Este estudio evidencia la colonización por dermatofitos en conejos domésticos, información importante para médicos veterinarios y propietarios de mascotas, al momento de estar en contacto con esta especie animal.


Subject(s)
Animals , Rabbits , Rabbits , Arthrodermataceae , Tinea
13.
Arq. ciênc. vet. zool. UNIPAR ; 20(1): 29-33, jan-mar. 2017. ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-849156

ABSTRACT

O pseudomicetoma dermatofítico (PMD) é uma profunda infecção dérmica, causada por fungos, sendo o Microsporum canis o agente etiológico mais frequentemente envolvido. O objetivo do presente trabalho é relatar um caso de pseudomicetoma em um gato persa e alertar os médicos veterinários para incluir a doença como diagnóstico diferencial em dermatopatias em gatos, principalmente os da raça persa. Um felino macho, da raça Persa, de três anos, veio para atendimento apresentando 22 nódulos subcutâneos, distribuídos em todo corpo, com diâmetros que variavam de 2 a 8 centímetros, inclusive dentro do pavilhão auricular direito. Realizou-se raspado cutâneo e citologia aspirativa por agulha fina, obtendo resultados pouco conclusivos. Após a realização de exérese cirúrgica de grande parte dos nódulos e encaminhamento para exame histopatológico, obteve-se o diagnóstico de PMD. Após tratamento com itraconazol 10mg/kg SID por 5 meses houve completa remissão do quadro. A excisão cirúrgica foi essencial para a resolução do quadro, que juntamente com o itraconazol sistêmico tornou o tratamento efetivo para o PMD.(AU)


Dermatophytic pseudomycetoma (DPM) is a deep skin infection. Microsporum canis is the etiologic agent most frequently involved in DPM. The purpose of this study is to report a case of DPM in a Persian cat and suggest the veterinarians to include the disease as a differential diagnosis in skin diseases in Persian cats. A male Persian cat, 3 years old, came to be attended at the Veterinary Hospital. The patient was presenting 22 subcutaneous nodules measuring 2-8 cm in diameter, in different parts of the body, including inside the right ear. Skin scraping and fine needle aspiration cytology were performed, yielding inconclusive results. After performing the surgical removal of most of the nodules and a skin biopsy, the diagnosis of DPM was obtained. The cat had a complete remission of the disease after the treatment with itraconazole 10mg/kg SID for 5 months. It is important to emphasize that, as a therapeutic point of view, surgical excision is considered essential, but not enough to establish a permanent cure. Since there can be recurrence, the systemic use of itraconazole is indicated to efficiently cure animals presenting DPM.(AU)


El pseudomicetoma dermatofitos (PMD) es una infección profunda de la piel causada por hongos, siendo el Microsporum canis el agente etiológico implicado con mayor frecuencia. El objetivo de este trabajo es presentar un caso de pseudomicetoma en un gato persa y alertar los médicos veterinarios para incluir la enfermedad como un diagnóstico diferencial de enfermedades de la piel en gatos, especialmente la raza persa. Un gato macho, de la raza persa, de tres años, llegó a la asistencia presentando 22 nódulos subcutáneos, distribuidos por todo el cuerpo, con diámetros que variaban de 2 a 8 cm, inclusive dentro de la oreja derecha. Se afeitó la piel y con citología por aspiración, con aguja fina, se obtuvo resultados poco conclusivos. Después de realizar la extirpación quirúrgica de la mayor parte de los nódulos y enrutamiento para el examen histopatológico, se ha obtenido el diagnóstico de PMD. Tras el tratamiento con itraconazol l0mg/kg SID, por cinco meses, hubo remisión completa. La escisión quirúrgica fue esencial para la resolución del cuadro, que junto con itraconazol sistémico se convirtió en un tratamiento eficaz para el PMD.(AU)


Subject(s)
Animals , Mycetoma/microbiology , Tinea/microbiology , Cats/microbiology , Microsporum
14.
CES med ; 31(1): 104-109, ene.-jun. 2017. graf
Article in Spanish | LILACS-Express | LILACS | ID: biblio-889544

ABSTRACT

Resumen El granuloma de Majocchi es una forma de infección atípica por dermatofitos con invasión de la dermis y el tejido celular subcutáneo, favorecida por el trauma de los folículos pilosos o la inmunosupresión del huésped. Siendo una infección poco común, se destaca en este artículo el caso de una paciente que luego de usar esteroides tópicos presentó pápulas y pústulas en vulva, sitio inusual de granuloma de Majocchi, siendo este el cuarto reporte a nivel mundial.


Abstract Majocchi granuloma is a form of atypical infection by dermatophytes with invasion of the dermis and subcutaneous tissue, favored by the trauma of hair follicles or host immunosuppression. Being a rare infection, in this article highlights the case of a patient with papules and pustules on the vulva after using topical steroids, unusual site Majocchi granuloma, which is the fourth global report.

15.
Rev. Nac. (Itauguá) ; 9(2): 12-19, 2017.
Article in Spanish | LILACS, BDNPAR | ID: biblio-884654

ABSTRACT

Introducción: las mascotas generalmente son reservorios y diseminadores de hongos causantes de tiñas en humanos. Objetivo: aislar e identificar hongos dermatofitos en el pelaje de gatos asintomáticos del área metropolitana de Asunción. Materiales y métodos: se incluyeron 68 gatos asintomáticos del área metropolitana de Asunción, cuyas edades estaban entre 1 mes y 14 años. Las muestras de pelos fueron obtenidas por el método del tapete de Mariat y Tapia, se cultivaron en Agar Sabouraud con cloramfenicol y cicloheximida (agar Mycosel) y se incubaron 21 días a 28 ºC. La identificación se basó en las características macroscópicas y microscópicas de las colonias. Resultados: se aislaron hongos dermatofitos en 13 gatos: 10 (14,7%) tenían Microsporum canis y 3 (4,4%) Microsporum gypseum. No se encontró diferencias significativas en cuanto a la presencia del hongo y las variables sexo, edad, hábitat y contacto con otros animales. Conclusión: en gatos de Asunción se aislaron Microsporun canis (14,7%) y Microscporum gypseum (4,4%).


Introduction: pets are generally reservoirs and disseminators of fungi causing "tinea" in humans. Objective: to isolate and identify dermatophyte fungi in hair of asymptomatic cats of the metropolitan area of Asunción. Materials and methods: 68 asymptomatic cats were included from the metropolitan area of Asunción, whose ages were between 1 month and 14 years. The hair samples were obtained by the Mariat and Tapia mat method, they were cultivated in Sabouraud Agar with chloramphenicol and cycloheximide (Mycosel agar) and incubated 21 days at 28 ºC. The identification was based on the macroscopic and microscopic characteristics of the colonies. Results: dermatophyte fungi were isolated in 13 cats: 10 (14.7%) had Microsporum canis and 3 (4.4%) Microsporum gypseum. No significant differences were found regarding the presence of the fungus and the variables sex, age, habitat and contact with other animals. Conclusion: Microsporum canis (14.7%) and Microscporum gypseum (4.4%) were isolated of Asunción cats.


Subject(s)
Animals , Male , Female , Cats , Animal Fur/microbiology , Microsporum/isolation & purification , Metropolitan Zones , Cross-Sectional Studies
16.
Rev. Nac. (Itauguá) ; 9(2): 04-11, 2017.
Article in Spanish | LILACS, BDNPAR | ID: biblio-884652

ABSTRACT

Introducción: los dermatofitos son las principales causas de micosis superficial. Su epidemiología es poco conocida en Paraguay. Objetivos: describir las especies prevalentes, tipos de tiñas y características según sexo y edad de pacientes que acudieron al Laboratorio Central de Salud Pública, Asunción- Paraguay,en el período 2000-2016. Materiales y Métodos: se estudiaron pacientes de todas las edades con diagnóstico de micosis superficiales. Las muestras procesadas fueron uñas, pelos y escamas epidérmicas Se realizaron exámenes directos con KOH y cultivos. La identificación fue por macro y microscopía de las colonias. Resultados: de 6.652 pacientes con sospecha de micosis superficiales, 803 (12%) fueron positivas para dermatofitos. Los aislamientos fueron Microsporumcanis (33,9%), Trichophytonrubrum (23,8%), Trichophytonmentagrophytes (22%), Trichophytontonsurans (12,6%), Microsporumgypseum (6,2%), Trichophytonverrucosum (1,4%), un aislamiento para Microsporummanuumy Epidermophytonfloccosum respectivamente. Los tipos de tiñas fueron: capitis (54,3%), corporis (27,1%) yunguium (9,8%), pedís (3,8%), cruris (3%) y manuum (2%). Conclusión: el hongo prevalente fue Microsporumcanis (33,9%) y la tiña más frecuente fue capitis.


Introduction: dermatophytes are the main causes of superficial mycosis. Its epidemiology is less known in Paraguay. Objectives: to describe the prevalent species, types of tinea and features according to sex and age of patients who attended the Central Laboratory of Public Health, Asunción-Paraguay, in the period 2000-2016. Materials and Methods: patients of all ages with a diagnosis of superficial mycosis were studied. The samples processed were nails, hairs and epidermal scales. Direct tests were carried out with KOH and cultures. The identification was by macro scopy and microscopy of the colonies. Results: of 6,652 patients with suspected superficial mycoses, 803 (12%) were positive for dermatophytes. The isolates were Microsporum canis (33.9%), Trichophyton rubrum (23.8%), Trichophyton mentagrophytes (22%), Trichophyton tonsurans (12.6%), Microsporum gypseum (6.2%), Trichophyton verrucosum (1,4%), isolation for Microsporum manuum and Epidermophyton floccosum respectively. The types of tinea were: capitis (54.3%), corporis (27.1%) and unguium (9.8%), pedis (3.8%), cruris (3%) and manuum (2%). Conclusion: The prevalent fungus was Microsporum canis (33.9%) and the most frequent tinea was capitis.


Subject(s)
Humans , Male , Female , Child , Adolescent , Adult , Middle Aged , Aged , Young Adult , Dermatomycoses/epidemiology , Paraguay/epidemiology , Tinea/epidemiology , Tinea Capitis/epidemiology , Cross-Sectional Studies , Retrospective Studies , Microsporum/isolation & purification
17.
Caracas; s.n; 20160000. 73 p. Tablas, Gráficos.
Thesis in Spanish | LILACS, LIVECS | ID: biblio-1368536

ABSTRACT

Las micosis superficiales son infecciones causadas por hongos que afectan la parte más superficial de la piel, mucosa, pelos y uñas. En los últimos años no se han creado nuevas drogas para el tratamiento de estas micosis. Por lo que es necesario investigar y desarrollar nuevos compuestos con actividad antifúngica. El aceite de girasol ozonizado, que aún está en estudio, representa una posible alternativa. Se planteó el objetivo de evaluar actividad antifúngica in vitro del aceite de girasol ozonizado en aislados clínicos de micosis superficiales. Se recopilaron cultivos de Candida albicans, Microsporum canis, Trichophyton rubrum, Fusarium spp., los cuales fueron ensayados para determinar su sensibilidad frente al aceite de girasol ozonizado, que consta de una fase oleosa y otra acuosa, empleando el método de referencia por microdilución en caldo según el CLSI y el método de unidades formadoras de colonias. Los datos obtenidos fueron analizados en base a valores numéricos y medidas de tendencia central. Las comparaciones se llevaron a cabo por la prueba estadística U de Mann-Whitney para análisis de variables no paramétricas. Por el método de unidades formadoras de colonias empleando la cepa de C. albicans ATCC90028 se obtuvieron tiempos de letalidad entre 15 y 60 minutos de exposición a las muestras ozonizadas. Por el método de microdilución en caldo se obtuvieron CIM de 0,4% ­ 0,75% para C. albicans; entre 0,75% y 1,5% para Fusarium spp., frente al aceite de girasol ozonizado. Frente a la fracción acuosa ozonizada se obtuvieron CIM entre 1,5% y 6,25% para C. albicans, entre 0,4% y 1,5% para Fusarium spp. Para los dermatofitos, las CIM y CFM frente a la porción acuosa ozonizada fueron ≤ 0,2% y ≤ 0,75%. Se concluye que el aceite de girasol ozonizado, tanto en su fracción oleosa como acuosa, tiene mayor actividad antifúngica que el aceite de girasol virgen.


Superficial fungal infections are caused by fungus that affect the most superficial part of the skin, mucosa, hair and nails. In recent years there have not created new drugs to treat these fungal infections. So it is necessary to research and develop new compounds with antifungal activity. Ozonated sunflower oil, which is still under study, represents a possible alternative. In order to evaluate in vitro antifungal activity of ozonized sunflower oil in clinical isolates of superficial mycosis, it was raised cultures of Candida albicans, Microsporum canis, Trichophyton rubrum, Fusarium spp., which were tested for their sensitivity to ozonized sunflower oil, consisting of an oil phase and another aqueous was collected using the reference method microdilution CLSI broth according to the method and the colony forming units. The data obtained were analyzed based on numerical values and measures of central tendency. Comparisons were carried out by the statistical U Mann-Whitney test for nonparametric analysis variables. By the method of colony forming units using strain C. albicans ATCC90028 lethality times were obtained between 15 and 60 minutes of exposure to ozonized samples. By the method of microdilution, MICs there were obtained 0.4% - 0.75% for C. albicans; between 0.75% and 1.5% for Fusarium spp., against ozonized sunflower oil. Opposite the ozonized aqueous fraction was obtained MIC between 1.5% and 6.25% for C. albicans, from 0.4% to 1.5% for Fusarium spp. For dermatophytes, MIC and MFC against the ozonized aqueous portion were ≤0.2% and ≤0.75%. It is concluded that ozonized sunflower oil, both aqueous and oil fractions has antifungal activitygreater than virgin sunflower oil.


Subject(s)
Humans , Male , Female , Trichophyton , Candida albicans , Onychomycosis , Dermatomycoses , Sunflower Oil , Microsporum , Antifungal Agents
18.
Invest. clín ; 57(1): 47-58, mar. 2016. tab
Article in Spanish | LILACS-Express | LILACS | ID: biblio-841098

ABSTRACT

Las micosis superficiales son muy comunes y por ello son motivo de consulta médica frecuente. El objetivo de este trabajo fue conocer la frecuencia de diagnóstico de las micosis superficiales en el Departamento de Micología del Instituto Nacional de Higiene “Rafael Rangel” en Caracas, Venezuela, durante 14 años (2001-2014). Se realizó un estudio transversal y retrospectivo de revisión de historias micológicas de pacientes con diagnóstico presuntivo de micosis superficial. Las muestras procesadas fueron uñas, pelos y escamas epidérmicas. La identificación de los hongos se realizó mediante observación macro y microscópica de las colonias y pruebas de identificación bioquímicas y fisiológicas, según requerimiento del agente aislado. Para la investigación de Malassezia spp. solo se realizó examen directo. De las 3228 muestras procesadas, 1098 (34%) resultaron positivas y su distribución según el agente etiológico fue: 79,5% dermatofitos; 10,9% levaduras; 5,1% hongos no dermatofitos y 4,5% Malassezia spp. El dermatofito más aislado fue el Complejo Trichophyton rubrum (70,1%), seguido del Complejo T. mentagrophytes (15,1%), Microsporum canis (9,4%) y Epidermophyton floccosum (4%). Las tiñas más frecuentes fueron: Tinea unguium (66,8%), seguida de Tinea pedis (16,4%) y Tinea capitis (8,1%). En el grupo de levaduras el Complejo Candida parapsilosis (37,5%) fue el más aislado y entre los hongos no dermatofitos el más frecuente fue Fusarium spp. (53,6%), seguido de Aspergillus spp. (19,6%) y Acremonium spp. (10,7%). La identificación del agente etiológico es fundamental para orientar un tratamiento adecuado. Esta casuística constituye un aporte importante para el conocimiento de la epidemiología de las micosis superficiales en nuestro país.


The superficial mycoses are very common infectious diseases and therefore are a frequent reason for medical consultation. The aim of this study was to determine the diagnostic frequency of superficial mycoses in the Mycology Department of the Instituto Nacional de Higiene “Rafael Rangel” during 14 years (2001-2014). A retrospective cross-sectional study was performed to review the mycological records of patients with presumptive diagnosis of superficial mycosis. Nails, hairs and epidermal scales were the processed samples. The identification of fungi was performed by macro and microscopic observation of colonies and biochemical and physiological tests, as required of the isolated agent. For the investigation of Malassezia spp. only direct examination was performed. Of the 3 228 samples processed, 1 098 (34%) were positive and their distribution according to the etiological agent was: dermatophytes 79.5%; 10.9% yeasts; non-dermatophytes fungi 5.1% and 4.5% Malassezia spp. The most frequently isolated dermatophyte was Trichophyton rubrum Complex (70.1%), followed by T. mentagrophytes complex (15.1%), Microsporum canis (9.4%) and Epidermophyton floccosum (4%). The most frequent ringworms were: Tinea unguium (66.8%), followed by Tinea pedis (16.4%) and Tinea capitis (8.1%). Candida parapsilosis complex (37.5%) was the most frequently isolated yeast and Fusarium spp. (53.6%) was the most isolated among non-dermatophyte fungi, followed by Aspergillus spp. (19.6%) and Acremonium spp. (10.7%). The identification of the etiological agent is essential to guide appropriate treatment. This study constitutes an important contribution to the knowledge of the epidemiology of superficial mycoses in our country.

19.
Clin. biomed. res ; 36(4): 230-241, 2016. tab
Article in Portuguese | LILACS | ID: biblio-831587

ABSTRACT

As dermatofitoses têm ocorrência mundial, sendo mais prevalentes em países de clima tropical e subtropical. Dados epidemiológicos indicam que essas micoses estão entre as infecções fúngicas de maior ocorrência. O quadro clínico mais comum de dermatofitose inclui despigmentação, placas anulares, prurido e perda de cabelo, com lesões tipicamente conhecidas como tineas, ocasionadas por fungos filamentosos dermatofíticos de três gêneros anamórficos: Microsporum, Trichophyton e Epidermophyton. O tratamento das dermatofitoses, em geral, está relacionado ao uso de antifúngicos tópicos e/ou sistêmicos, apresentando como problemática o surgimento de espécies multirresistentes. Esta revisão aborda as dermatofitoses e seus agentes etiológicos de forma aprofundada em aspectos epidemiológicos, apresentando a importância clínica do tema, com ênfase na causa, prevenção, tratamento e prognóstico dessa micose cutânea (AU)


Dermatophytoses have worldwide occurrence with higher prevalence in tropical and subtropical countries. Epidemiological data show that these mycoses are among the most frequent fungal infections. The most common symptoms of dermatophytoses include depigmentation, annular plaques, itching and hair loss, with lesions such as tinea, caused by dermatophytic filamentous fungi of three anamorphic genera: Microsporum, Trichophyton and Epidermophyton. Topical and/or systemic antifungalmedications are used in the treatment of dermatophytoses in general, resulting in problems such as the emergence of multidrug-resistant species. This review discusses dermatophytoses and their etiological agents with a focus on epidemiological aspects, presenting the clinical importance of the issue, with emphasis on cause, prevention, treatment and prognosis of this skin mycosis (AU)


Subject(s)
Humans , Antifungal Agents/therapeutic use , Arthrodermataceae/classification , Tinea , Coinfection , Tinea/classification , Tinea/diagnosis , Tinea/drug therapy , Tinea/epidemiology , Tinea/etiology , Tinea/microbiology , Tinea/prevention & control
20.
Rev. Soc. Venez. Microbiol ; 35(1): 31-34, nov. 2015.
Article in Spanish | LILACS-Express | LILACS | ID: lil-780211

ABSTRACT

Debido al pleomorfismo y variabilidad macroscópica que presenta el género Trichophyton, 1os métodos de identificación basados exclusivamente en caracteres morfológicos no son suficientes para su clasificación. El objetivo fue evaluar las características morfológicas y bioquímicas de aislados clínicos identificados como Trichophyton spp. Se estudiaron 98 cultivos, identificados previamente por macro y micromorfología y se reevaluaron por: microcultivo en lámina, perforación del pelo in vitro, hidrólisis de urea y uso de agares Trichophyton y BCP-MS-G. El análisis morfológico y las pruebas de hidrólisis de urea y perforación del pelo arrojaron 51% T. rubrum, 24% T. mentagrophytes, 16,6% T. interdigitale, 7% T. tonsurans y 2% Trichophyton spp. La prueba de urea evidenció un error de 9,4%. La evaluación mediante el BCP-MS-G permitió diferenciar T. rubrum tipo A algodonoso de T. interdigitale, e identificar Trichophyton spp. como T. rubrum tipo B granular. La concordancia entre la evaluación previa y la reevaluación fue de un 70% y entre las pruebas morfofisiológicas y BCP-MS-G fue de 96%. Se detectó un 30% de sobrediagnóstico de T. rubrum. Se sugiere el empleo del medio BCP-MS-G para diferenciar T. interdigitale de T. rubrum tipo A y otros biotipos de T. rubrum tipo B.


Due to the pleomorphism and macroscopic variability of the Trichophyton genus, identification methods based solely on the morphological characteristics are insufficient for its classification. The objective was to evaluate the morphological and biochemical characteristics of clinical isolates of Trichophyton spp. The study included 98 isolates previously identified by macro and micromorphology that were reassessed by means of microculture sheet, in vitro hair perforation, urea hydrolysis and culture on Trichophyton and BCP-MS-G agars. For morphological analysis, urea hydrolysis tests and in vitro hair perforation results were: T. rubrum 51%, T. mentagrophytes 24%, T. interdigitale 16.6%, T. tonsurans 7% and Trichophyton spp. 2%. The urea hydrolysis test showed 9.4% error. Culture on the BCP-MS-G medium allowed to differentiate T. rubrum cottony A type from T. interdigitale and the identification of Trichophyton spp. as T. rubrum granular B type. The agreement between the previous assessment and reassessment was 70% and when morphophysiological and BCP-MS-G culture were considered, agreement reached 96%. There was 30% overdiagnosis for T. rubrum. Cultures on BCP-MS-G agar are suggested to differentiate T. interdigitale from T. rubrum A type and other biotypes of T. rubrum B type.

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