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1.
Braz. j. biol ; 84: e251733, 2024. tab, graf
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1355875

ABSTRACT

Abstract Mining is vital for human sustenance and a crucial sector in the state economy. However, its impacts on the environment and biodiversity cannot be underestimated. Which are potent to the attract government's attention. Environment and wildlife are subject to the harmful impacts of mining and its related activities. In this study, districts, namely Mardan and Mohmand have been targeted with respect to mining impacts. The assessment was carried out on wildlife adversely affected by the mining sector. The fauna has been keenly observed to bring the calculated risks and threat perception of the regional wildlife. Total 9 species of mammals, 21 species of birds, were recorded in District Mardan. While in District Mohmand 2 species of mammals, 9 species of birds, and 4 species of reptiles were studied. The Study explored that mining primarily responsible for land degradation. Which lead to food and agriculture losses. Several other factors like blasting, pollution, hunting, deforestation, habitat loss was also observed. Deforestation surfaced one of the major causes for extinction of fauna in the said region. preemptive measures are needed to seize the man-made catastrophe.


Resumo A mineração é vital para o sustento humano e um setor crucial na economia do Estado. No entanto, seus impactos sobre o meio ambiente e a biodiversidade não podem ser subestimados, visto serem potentes para atrair a atenção do governo. O meio ambiente e a vida selvagem estão sujeitos aos impactos prejudiciais da mineração e de suas atividades relacionadas. Neste estudo, os distritos, nomeadamente Mardan e Mohmand, foram selecionados ​​no que diz respeito aos impactos da mineração. A avaliação foi realizada em animais selvagens afetados negativamente pelo setor de mineração. A fauna tem sido observada atentamente para trazer os riscos calculados e a percepção de ameaça à vida selvagem regional. Um total de nove espécies de mamíferos, 21 espécies de pássaros, foi registrado no Distrito Mardan. Enquanto no Distrito Mohmand, duas espécies de mamíferos, nove espécies de pássaros e quatro espécies de répteis foram estudadas. O estudo explorou essa mineração que é principalmente responsável pela degradação do solo, acarretando perdas de alimentos e na agricultura. Vários outros fatores como explosões, poluição, caça, desmatamento, perda de habitat também foram observados. O desmatamento veio à tona como uma das principais causas de extinção da fauna da região, e tem-se que medidas preventivas são necessárias para dimensionar a catástrofe provocada pelo homem.


Subject(s)
Humans , Animals , Biodiversity , Animals, Wild , Mining , Pakistan
2.
Braz. j. biol ; 83: e249847, 2023. tab, graf
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1339414

ABSTRACT

Abstract The Indian crested porcupine (Hystrix indica) is a vertebrate pest of agricultural lands and forest. The study was aimed to report the damage to local crops by the Indian crested porcupine (Hystrix indica) in the Muzaffarabad District. A survey was conducted to identify the porcupine-affected areas and assess the crop damage to the local farmers in district Muzaffarabad Azad Jammu and Kashmir (AJK) from May 2017 to October 2017. Around 19 villages were surveyed, and a sum of 191 semi-structured questionnaires was distributed among farmers. Crop damage was found highest in village Dhanni where a porcupine destroyed 175 Kg/Kanal of the crops. Regarding the total magnitude of crop loss, village Danna and Koomi kot were the most affected areas. More than half (51.8%) of the respondents in the study area suffered the economic loss within the range of 101-200$, and (29.8%) of the people suffered losses in the range of 201-300$ annually. Among all crops, maize (Zea mays) was found to be the most damaged crop ranging between 1-300 Kg annually. In the study area, porcupine also inflicted a lot of damages to some important vegetables, including spinach (Spinacia oleracea), potato (Solanum tuberosum) and onion (Allium cepa). It was estimated that, on average, 511Kg of vegetables are destroyed by porcupine every year in the agricultural land of Muzaffarabad. It was concluded that the Indian crested porcupine has a devastating effect on agriculture which is an important source of income and food for the local community. Developing an effective pest control strategy with the help of the local government and the Wildlife department could help the farmers to overcome this problem.


Resumo O porco-espinho de crista indiano (Hystrix indica) é uma praga vertebrada de terras agrícolas e florestais. No estudo atual, o dano às plantações locais pelo porco-espinho de crista indiano (Hystrix indica) foi relatado pela primeira vez no distrito de Muzaffarabad. O estudo foi projetado para investigar a perda econômica causada pelo porco-espinho de crista indiano (Hystrix indica) nos distritos de Muzaffarabad, Azad Jammu e Caxemira (AJK) de maio de 2017 a outubro de 2017. Um estudo baseado em pesquisa foi conduzido para identificar as áreas afetadas por porcos-espinhos e avaliar os danos às colheitas para os agricultores locais. Cerca de 19 aldeias foram pesquisadas e um total de 191 questionários semiestruturados foi distribuído entre os agricultores. Os danos às colheitas foram encontrados mais intensamente na aldeia Dhanni, onde um porco-espinho destruiu 175 kg / Kanal das colheitas. Em relação à magnitude total da perda de safra, as aldeias Danna e Koomi Kot foram as áreas mais afetadas. Mais da metade (51,8%) dos entrevistados na área de estudo sofreu perdas econômicas na faixa de 101-200 $, e 29,8% das pessoas sofreram perdas na faixa de 201-300 $ anualmente. Entre todas as culturas, o milho (Zea mays) foi considerado a cultura mais danificada, variando entre 1-300 kg anualmente. Na área de estudo, o porco-espinho também causou muitos danos a alguns vegetais importantes, incluindo espinafre (Spinacia oleracea), batata (Solanum tuberosum) e cebola (Allium cepa). Estimou-se que, em média, 511 kg de vegetais são destruídos pelo porco-espinho todos os anos nas terras agrícolas de Muzaffarabad. Concluiu-se que o porco-espinho de crista indiano tem um efeito devastador na agricultura, que é importante fonte de renda e alimento para a comunidade local. O desenvolvimento de uma estratégia eficaz de controle de pragas com a ajuda do governo local e do Departamento de Vida Selvagem pode ajudar os agricultores a superar esse problema.


Subject(s)
Humans , Animals , Porcupines , Pakistan , Crops, Agricultural , Agriculture , Animals, Wild
3.
Rev. psicanal ; 28(1): 103-120, Abril 2021.
Article in Portuguese | LILACS | ID: biblio-1252999

ABSTRACT

O presente trabalho parte da ideia de caracterizar o disruptivo no pensamento freudiano. Como ponto de partida, toma o trabalho de 1914, À guisa de introdução ao narcisismo, por reconhecer nele um momento primeiro de ruptura na teoria pulsional vigente: libido do Eu versus libido objetal. Durante o trajeto, sinaliza marcas desse processo e direciona-se para o disruptivo que se instala em termos metapsicológicos, com maior consistência, com o advento da pulsão de morte. A pulsão de destruição, como agente do disruptivo em sua relação com Eros, desenhará caminhos que permitem vislumbrar destinos tanáticos ou criativos. Com essa concepção metapsicológica como indicador, busca-se refletir a respeito da interação entre o disruptivo da pandemia viral e o disruptivo da virulência do racismo e seus desdobramentos criativos na efetivação, pelo coletivo da humanidade, de posturas antirracistas. Tal contexto alberga uma interrogação pontual: como a pandemia, em seu efeito disruptivo, está relacionada com a percepção em toda a sua sensorialidade, em grande escala, de norte a sul, daquilo que mantinha-se parcialmente silencioso e invisível, o racismo? (AU)


The present article begins from the idea of characterize the disruptive in the freudian's thoughts. Is takes as a starter point the work of 1914, On narcissism: an introduction, for recognize it as a first moment of rupture in the current drive theory: self libido versus object libido. In this path, it signals marks of this process and orientate to the disruptive that develops in metapsychological terms, with great consistency, with the advent of the death drive. The destruction drive, as a disruptive agent, in its relation with Eros, will draw paths that allow glimpse its tanatic fate or criative fate. From this metapsychological conception, as an indicator, seeks to reflect the interaction between the disruptive in the viral pandemic and the disruptive in the racism virulence, and its criatives developments in the effectuation of anti-racist postures, by the humanity collective. Context that holds an punctual interrogation: how the pandemic, with its disruptive effect, is related with the perception in all its sensoriality, in big scale, from north to south, with what was, in part, silence and inivisible: the racism? (AU)


El objetivo inicial del presente trabajo es caracterizar lo disruptivo en el pensamiento freudiano. Se toma como punto de partida el célebre texto de 1914 Introducción del narcisismo por reconocer en él un primer momento de ruptura en la teoría pulsional vigente hasta ese momento, que distinguía la libido del Yo y la libido de objeto. En ese recorrido, se irán señalando marcas de dicho proceso orientándose hacia lo disruptivo, que se instalará con mayor consistencia, en términos metapsicológicos, con el advenimiento de la pulsión de muerte. La pulsión de destrucción, como agente de lo disruptivo, en su relación con Eros, trazará caminos que permiten vislumbrar sus destinos tanáticos o creativos. Tomando esa concepción metapsicológica como indicador, busco reflejar la interacción entre lo disruptivo de la pandemia viral y lo disruptivo de la virulencia del racismo, así como sus desdoblamientos creativos en la adopción de posturas antirracistas por parte del colectivo humano. En este contexto se plantea una interrogación puntual: ¿cómo la pandemia, con su efecto disruptivo, está relacionada con la percepción en toda su sensorialidad, en gran escala, de norte a sur, de aquello que, en parte, se mantenía silencioso e invisible, el racismo?


Subject(s)
Pandemics/prevention & control , Racism/psychology , Rupture/psychology , Virulence , Drive , Narcissism
4.
Article in Chinese | WPRIM | ID: wpr-906529

ABSTRACT

For rheumatoid arthritis, glucocorticoids or immunosuppressive agents are currently used in clinical treatment, but long-term use of these drugs has large side effect on humans, and immunosuppressive agents are expensive. To a certain extent, its wide application is limited. The treatment of rheumatoid arthritis with traditional Chinese medicine(TCM) has a long history and little toxic and side effect, but its specific mechanism of action needs further exploration. The process of autophagy is an active biological process in which cells themselves are stimulated by the outside world through intracellular signal transduction to maintain a stable internal environment. Its abnormality is involved in the occurrence of many diseases. At present, studies have shown that abnormal autophagy is closely related to the occurrence and development of rheumatoid arthritis, which can interfere with the pathological changes of RA pannus formation, synovial inflammation and bone destruction and affect the disease process. In recent years, many studies have found that traditional Chinese medicine and its active ingredients can affect the pathological development of rheumatoid arthritis by regulating autophagy. This article investigates the relevant literature on the intervention of rheumatoid arthritis by regulating autophagy through using TCM, with a view to providing new ideas for basic research, new drug development and clinical treatment of rheumatoid arthritis.

5.
Article in English | WPRIM | ID: wpr-904308

ABSTRACT

@#BACKGROUND: To investigate the clinical characteristics and risk factors of human immunodeficiency virus (HIV)-negative patients with Talaromyces marneffei (T. marneffei) infection. METHODS: We retrospectively collected the clinical information of HIV-negative patients with T. marneffei infection from January 1, 2010 to June 30, 2019, and analyzed the related risk factors of poor prognosis. RESULTS: Twenty-five cases aging 22 to 79 years were included. Manifestations of T. marneffei infection included fever, cough, dyspnea, chest pain or distress, lymphadenopathy, ear, nose, and throat (ENT) and/or skin lesions, bone or joint pain, edema and pain in the lower extremities, digestive symptoms, icterus, malaise, and hoarseness. Two cases had no comorbidity, while 23 cases suffered from autoimmune disease, pulmonary disease, cancer, and other chronic diseases. Sixteen cases had a medication history of glucocorticoids, chemotherapy or immunosuppressors. Pulmonary lesions included interstitial infiltration, nodules, atelectasis, cavitary lesions, pleural effusion or hydropneumothorax, bronchiectasis, pulmonary fibrosis, pulmonary edema, and consolidation. The incidence of osteolytic lesions was 20%. Eight patients received antifungal monotherapy, and 11 patients received combined antifungal agents. Fifteen patients survived and ten patients were dead. The Cox regression analysis showed that reduced eosinophil counts, higher levels of blood urea nitrogen (BUN), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactic dehydrogenase (LDH), myoglobin (Mb), procalcitonin (PCT), and galactomannan were related to poor prognosis (hazard ratio [HR]>1, P<0.05). CONCLUSIONS: Bone destruction is common in HIV-negative patients with T. marneffei infection. Defective cell-mediated immunity, active infection, multiple system, and organ damage can be the risk factors of poor prognosis.

6.
J Cancer Res Ther ; 2020 Sep; 16(5): 1082-1087
Article | IMSEAR | ID: sea-213758

ABSTRACT

Aims: We aimed to investigate the feasibility, safety, and efficacy of radiofrequency ablation (RFA) combined with percutaneous vertebroplasty (PVP) for treating VX2 vertebral metastases with posterior margin destruction in a rabbit model. Materials and Methods: Sixty rabbit models of VX2 vertebral metastases with posterior margin destruction were constructed through computed tomography (CT)-guided percutaneous puncture and randomly divided into four groups of 15 rabbits each: Groups A, RFA+PVP; B, PVP; C, RFA; and D, control. Five rabbits in each group were sacrificed within 24 h of the procedure. Pathological examination and immunohistochemical staining revealed the presence of a biomembrane barrier at the tumor edge; furthermore, bone cement leakage into the spinal canal was observed. The survival time of the remaining rabbits per group was observed, and the differences were analyzed. Results: CT scans of Group A and C rabbits revealed a low-density band around the tumor ablation region. Bone cement leakage rate significantly differed between Groups A and B (20% vs. 100%; P < 0.05). The average postoperative survival times of Group A, B, C, and D rabbits were 16.72 ± 0.93, 7.26 ± 0.75, 7.80 ± 1.30, and 3.84 ± 1.24 days, respectively, showing a significant difference between Group A and the remaining groups (P < 0.05). Conclusions: The biomembrane barrier formed at the tumor edge after RFA can prevent bone cement leakage into the spinal canal, reducing spinal cord injury and prolonging the survival time

7.
Article | IMSEAR | ID: sea-210211

ABSTRACT

Background:Suicide is an intentional fatal act of self-destructionand largely preventable phenomenon. Early Identification of suicide risk factors, proactive preventive steps and therapeutic interventions tend to reduce robustly its epidemiological trends including associated high mortality around the world. Objective:This review study aimed to critically describe the identified potential risk factors underlying suicide together with a specific focus on its relevant preventive and management strategies. Methods:A selective e-searches of Google Scholar, PubMed/MEDLINE and ScienceDirect of relevant English literature (2000-2019) was conducted by using keywords and Boolean operators, and following exclusion and inclusion criteria included 115 most influentialarticles for this critical review.Results:Suicide is a global preventable phenomenon determined by multiple interconnected risk factors and mechanisms embedded in several levels; population (indigenous people and social media), individual (distal predisposing factors), developmental (mediating factors) and proximal (precipitating factors).Evidently suicide needs multimodal intervention approach in terms of universal, selective and indicated prevention. Conclusion:Suicide is a global heterogeneous phenomenon and needs continuing concerted efforts of multidisciplinary health team and multi-sector stakeholders because its risk pathways and protective factors dynamically changes overtime across the world

8.
Chinese Journal of Traumatology ; (6): 314-318, 2020.
Article in English | WPRIM | ID: wpr-879643

ABSTRACT

In this paper, we review the results of previous studies and summarize the effects of various factors on the regulation of bone metabolism in traumatic bone infections. Infection-related bone destruction incorporates pathogens and iatrogenic factors in the process of bone resorption dominated by the skeletal and immune systems. The development of bone immunology has established a bridge of communication between the skeletal system and the immune system. Exploring the effects of pathogens, skeletal systems, immune systems, and antibacterials on bone repair in infectious conditions can help improve the treatment of these diseases.


Subject(s)
Anti-Bacterial Agents/administration & dosage , Bone and Bones/metabolism , Cellular Microenvironment , Humans , Immune System/immunology , Lymphocyte Subsets/immunology , Osteitis/microbiology , Osteoblasts/physiology , Osteoclasts/physiology , Staphylococcal Infections
9.
Journal of Medical Biomechanics ; (6): E595-E601, 2020.
Article in Chinese | WPRIM | ID: wpr-862352

ABSTRACT

Objective To conduct dynamic impact failure test of rabbit single vertebra, and make comparison with the static compression experiment, so as to study damage mechanism of the vertebral body under the axial impact. Methods The voltage waveform diagram of the force sensor and the detailed process of the vertebral impact were obtained by the oscilloscope and high-speed photography through the drop hammer dynamic impact experimental device. Results The average static load of the thoracic and lumbar vertebra were 910 N and 947 N, respectively; the average dynamic load of the thoracic and lumbar vertebra were 1 196 N and 1 026 N, respectively; the average thoracic and lumbar dynamic load coefficients were 1.37 and 1.08; under static load, the average stress of the thoracic and lumbar vertebra was 15.28 MPa and 12.51 MPa, respectively; under dynamic load, the average stress of the thoracic and lumbar vertebra was 20.03 MPa and 13.56 MPa; during dynamic impact, the mean longitudinal strain and transverse strain was -0.3 and -0.005 (compression); under dynamic conditions, the destruction energy of vertebrae increased from 0 J to 4.4 J. Conclusions Under dynamic and static experimental conditions, the dynamic load of the same vertebral body was greater than that of the static load; the average dynamic load coefficient of the thoracic vertebra was larger than that of the lumbar vertebra; the equivalent stress of the thoracic vertebra was greater than that of the lumbar vertebra; the axial strain of vertebra under impact was greater than the transverse strain; energy growth of the vertebral body presented a slow at first and then a rapid changing process. The research findings can provide some guidance for prevention and rehabilitation of human vertebral body injury in clinic.

10.
Article in Chinese | WPRIM | ID: wpr-848066

ABSTRACT

BACKGROUND: Studies have shown that the loss of sex combing protein 1 (Asxl1) can lead to the occurrence of bone dysplasia and bone defects, but the relationship between this factor and bone destruction in the microenvironment of apical periodontitis has not been reported. OBJECTIVE: To study the effect of Asxl1 on proliferation and differentiation of osteoblasts in an inflammatory microenvironment. METHODS: MC3T3-E1 cells were excited by lipopolysaccharide to establish an in vitro inflammatory microenvironment. The best concentration and optimal action time of lipopolysaccharide were screened by cell counting kit-8 test. MC3T3-E1 cells were then stimulated with 20 mg/L lipopolysaccharide for 24 hours. The expression levels of Asxl1 protein and mRNA were detected by immunofluorescence and real-time PCR, respectively. After lipopolysaccharide stimulated the formation of inflammatory microenvironment, Asxl1-Si RNA was transfected for 24 hours, cell counting kit-8 was applied to detect the activity of cell proliferation, and real-time PCR was used to detect the expression levels of Asxl1 and osteogenic related genes ALP and RUNX2 mRNA. RESULTS AND CONCLUSION: After lipopolysaccharides stimulation of MC3T3-E1 cells, the expression levels of Asxl1 protein and mRNA were decreased. Under the inflammatory microenvironment, the proliferation activity of MC3T3-E1 cells transfected with Asxl1-Si RNA for 24 hours was significantly decreased, and the expression levels of Asxl1, ALP and RUNX2 mRNA were markedly decreased. These findings indicate that Asxl1 may influence the proliferation and differentiation of osteoblasts by involvement in the process of inflammatory reaction, thereby participating in bone destruction.

11.
Article in Chinese | WPRIM | ID: wpr-847970

ABSTRACT

BACKGROUND: MicroRNA-21 (miR-21) is a regulator of osteoclastogenesis and a promoter of osteoclast differentiation, but its role in periodontitis remains unclear. OBJECTIVE: To investigate whether miR-21 is involved in bone destruction in periodontitis. METHODS: Real-time PCR was used to detect and analyze the differential expression of miR-21 in periodontitis samples. Using liposome transfection method, miR-21 mimics (up-regulating miR-21) or miR-21 inhibitor (down-regulating miR-21) was used to transfect osteoclasts. Expressions of miR-21 and bone destruction markers TRAP and CTSK were detected by real-time PCR. Cell counting kit-8 was used to detect the miR-21 effect on osteoclast proliferation. RESULTS AND CONCLUSION: (1) MiR-21 expression increased in periodontitis samples. (2) When miR-21 mimics was transfected into osteoclasts, miR-21, TRAP and CTSK mRNA expression increased; when miR-21 inhibitor was transfected into osteoclasts, miR-21, TRAP and CTSK mRNA expression decreased. (3) Transfection with miR-21 mimics promoted the proliferation of osteoclasts, while transfection with miR-21 inhibitor inhibited the proliferation of osteoclasts. To conclude, miR-21 can be used as an important target for the treatment of periodontitis.

12.
Article in Chinese | WPRIM | ID: wpr-847448

ABSTRACT

BACKGROUND: In recent years, the PI3K/AKT signaling pathway is closely related to bone destruction-related diseases. Inhibitors targeting this signaling pathway are also undergoing extensive research, which provides new ideas for clinical treatment of bone destruction-related diseases. OBJECTIVE: To review the research of PI3K/AKT signaling pathway in bone destruction. METHODS: WanFang and PubMed databases were searched for relevant articles published from January 1998 to August 2019. The retrieval key words were “PI3K/Akt signaling pathway; osteoclasts; osteoblasts; bone destruction” in Chinese and English, respectively. To exclude duplicate studies by reading the title and abstract, finally 67 articles were included in result analysis. RESULTS AND CONCLUSION: An appropriate balance between osteoblast and osteoclast-mediated osteogenesis and bone resorption is necessary to maintain bone homeostasis, and an imbalance between these two biological processes will result in bone destructi on. PI3K/Akt signaling pathway is a signal transduction pathway that regulates cell activities and plays an important role in cell proliferation, apoptosis and differentiation. The PI3K/Akt signaling pathway is involved in the inhibition of osteoporosis by promoting osteoblast proliferation, differentiation and bone formation. These results reveal that further exploration on the inhibitors of the PI3K/Akt signaling pathway related to bone destruction will provide new ideas for drug therapy in clinical practice.

13.
Article in Chinese | WPRIM | ID: wpr-843931

ABSTRACT

Objective: To improve the traditional teaching technique of using probe to destroy the brain tissue and spinal cord of toads. Methods:On the basis of the traditional teaching content, the distance of the probe entering the skull cavity and the specific feeling mark of destroying the brain tissue and spinal cord were added. Students were randomly divided into normal group and improved group according to the class. We observed the success rate and time spent on both the first and the second operations in the two groups. Results: The success rate of destroying the brain tissue and spinal cord in the improved group was high (93.2% vs. 72.3% for the first time). The secondary success rate was 97.7% vs. 85.1%, and it took less time (311.7±89.3) seconds vs. (511.6±171.1) seconds for the initial operation, The secondary operation time was (161.3±63.5) seconds vs. (266.0±98.2) seconds, with significant differences between the two groups (P<0.05).Conclusion: After the improvement of the content of destroying the brain tissue and spinal cord of toads in traditional teaching, it is easy to be mastered by students, with high success rate, less time spent, greater consistency with the 3R principle, and easier to be popularized and applied in teaching.

14.
Article in Chinese | WPRIM | ID: wpr-799091

ABSTRACT

Objective@#To investigate the effects of modified acidic fibroblast growth factor (MaFGF) mediated by nanoliposomes combined with ultrasound-targeted microbubble destruction (UTMD) on left ventricular systolic function in early diabetes mellitus(DM) rats.@*Methods@#The nanoliposomes containing MaFGF(MaFGF-nlip) were prepared by reverse phase evaporation method. Among 60 male Sprague Dawley (SD) rats, 50 rats were randomly selected and were induced to be DM models by streptozotocin(STZ) through intraperitoneal injecting, the other 10 rats as control group. Then DM rats were randomly divided into 4 groups: DM model group, MaFGF solution group, MaFGF-nlip group and MaFGF-nlip+ UTMD group. After the successful induction of DM model, the intervention was performed twice a week.After 12 weeks of intervention, all rats underwent conventional echocardiography and velocity vector imaging (VVI). Left ventricular ejection fraction (LVEF) and left ventricular fraction shortening(LVFS) were measured by conventional echocardiography. The mean peak systolic radial velocity (Vs), radial strain (Sr) and radial strain rate (SRr) of six walls at the papillary muscle level were measured in left ventricular short-axis view by VVI. At last, myocardial tissue of all rats were stained with Sirius red to evaluate myocardial interstitial fibrosis. The level of myocardial apoptosis was evaluated by TUNEL staining, and the changes of myocardial ultrastructure were observed by transmission electron microscopy.@*Results@#The prepared MaFGF-nlip were more rounded, evenly dispersed, and of good stability and high encapsulation efficiency. Twelve weeks later after intervention, LVEF, LVFS, Vs, Sr and SRr in the DM model group were significantly lower than those in the control group (all P<0.05). LVEF, LVFS, Vs, Sr and SRr in the MaFGF-nlip+ UTMD group were significantly higher than those of the DM model group and other intervention groups (all P<0.05). The results of Sirius red staining and Tunel staining showed that CVF and AI in the DM model group were significantly higher than those in the control group (all P<0.05). For MaFGF-nlip+ UTMD group, CVF and AI were significantly decreased compared with the DM model group and other intervention groups(all P<0.05). According to the results of transmission electron microscopy, compared with the DM model group, the improvement of myocardial ultrastructure was the most obvious in the MaFGF-nlip+ UTMD group.@*Conclusions@#MaFGF delivered by using nanoliposomes combined with UTMD can improve the left ventricular systolic function in diabetic rats by inhibiting the myocardium cardiac fibrosis and reducing the cardiomyocyte apoptosis.

15.
Article in Chinese | WPRIM | ID: wpr-826328

ABSTRACT

Ultrasound contrast agent microbubbles combined with low frequency ultrasound named as low-frequency ultrasound-targeted microbubble destruction technology has become an effective and non-invasive anti-tumor therapy for deep tumors.It can enhance the efficacies of chemotherapy,gene therapy,immunotherapy,and anti-angiogenic therapy by improving cell membrane permeability and destroying tumor neovasculature.It can be applied to sonodynamic therapy and realize multimodal synergistic therapy on the basis of nanoparticles,which increases the anti-tumor efficiency and offers a promising target therapy for tumors.


Subject(s)
Contrast Media , Genetic Therapy , Humans , Microbubbles , Neoplasms , Ultrasonography
16.
Article in Chinese | WPRIM | ID: wpr-777985

ABSTRACT

Objective@# To explore the clinical, imaging and pathological characteristics of mandibular peripheral primitive neuroectodermal tumors, and to review relevant literature to improve the understanding and diagnosis of pPNET in mandible.@* Methods@# The clinical and imaging features, pathological examination, treatment and prognosis of a case of mandibular pPNET diagnosed and treated at the First Affiliated Hospital of Xinjiang Medical University were observed, and analyzed a literature review. @* Results @#The patient′s clinical manifestations were an enlarged mass of the mandible, hard texture, unclear borders, involving loose teeth, and numbness of the lower lip; CT and MRI showed osteolytic and aggressive growth patterns. The mandibular tumor was resected and the mandibular partial truncated resection was performed on the titanium plate. Postoperative pathological sections showed small round cell tumors under HE staining and Vimentin and Fli-1 were positive, and the pathological diagnosis was pPNET. The patient did not undergo chemoradiotherapy after surgery and died of tumor recurrence after 9 months of follow-up. A review of the relevant literature revealed that pPNETs are a group of small round cell tumors, which are more common in children and adolescents. pPNETs have a high degree of malignancy, a short course of disease and fast metastasis. The main route of metastasis is through the blood circulation. Most patients die within 2 years, the 3-year survival rate is only 30%, and the 5-year survival rate is less than 10%. Imaging is generally nonspecific; therefore, pPNETs are easily misdiagnosed. The final tumor type is determined by pathological HE staining and immunohistochemical characteristics. Current treatment methods are mainly complete surgical resection combined with postoperative radiotherapy and chemotherapy, but it is critical to provide individualized treatment to patients when necessary.@*Conclusion@# pPNETs have a high degree of malignancy, easy recurrence and poor prognosis, so early diagnosis and treatment are extremely important.

17.
Article in Chinese | WPRIM | ID: wpr-772646

ABSTRACT

OBJECTIVE@#To observe the effect of Bruton's tyrosine kinase (BTK) on the proliferation and differentiation of osteoclasts and to explore the mechanism of BTK on bone destruction in periapical periodontitis.@*METHODS@#After RAW264.7 cells induced with 100 ng·L⁻¹ receptor activator for nuclear factor-κB ligand (RANKL) for 5 days, osteoclast induction was confirmed by light microscopy, tartrate-resistant acid phosphatase (TRAP) staining, and quantitative real-time PCR (RT-qPCR). Then, BTK-small interfering RNA (BTK-siRNA) was transfected into cells induced for 5 days. After 24 h, the expression of TRAP mRNA was measured using RT-qPCR, and the proliferation and differentiation of osteoclasts were detected using CCK-8 and TRAP activity assay. Statistical analysis was performed.@*RESULTS@#After RAW264.7 was induced with RANKL for 5 days, a large number of round, ellipse, irregularly protuberant, and TRAP-positive macrophages were observed under light microscopy. The expression of TRAP mRNA significantly reduced after 24 h of BTK-siRNA transfection (P<0.05). The detection of CCK-8 and TRAP activities showed that the proliferation and differentiation of osteoclasts significantly decreased (P<0.05).@*CONCLUSIONS@#Silencing of BTK can inhibit the proliferation and differentiation of osteoclasts. BTK can be used as a new target for the inhibition of osteoclasts.


Subject(s)
Agammaglobulinaemia Tyrosine Kinase , Cell Differentiation , Cell Proliferation , Macrophages , Osteoclasts , RANK Ligand
18.
Article in Chinese | WPRIM | ID: wpr-745139

ABSTRACT

Objective To explore the application value of IL-8 monoclonal antibody microbubble combined with ultrsound targeted microbubble destruction ( UTMD) on alleviating myocardial ischemia reperfusion/injury ( MIRI) in rabbits .Methods Forty-two rabbits were randomly divided into closed chest group ( n =7) ,open chest control group ( n = 7) and ischemia-reperfusion ( I/R) group ( n = 28) .I/R group were randomly divided into 30 min reperfusion group( n =7) ,60 min reperfusion group( n =7) ,120 min reperfusion group ( n = 7 ) and 180 min reperfusion group ( n = 7 ) .All rabbits were examined by electrocardiogram , echocardiography and HE staining after MIRI . Targeted myocardial contrast echocardiography ( MCE) was performed and ELISA was used to detect IL-8 content in rabbit myocardium before and after UTMD . Results Electrocardiogram and wall motion returned to normal at 60 min after reperfusion .Targeted MCE showed that with the prolongation of reperfusion after I/R ,the video intensity of myocardium in reperfusion area increased gradually , reaching its peak at 120 min and 180 min after reperfusion .After UTMD ,the video intensity decreased ,and the change rate of video intensity in 30 min reperfusion group was higher than those in other reperfusion groups(all P<0 .05) .The content of IL-8 and its neutralization rate in the ELISA results were consistent with the video intensity and rate of change of targeted MCE .HE staining and scanning electron microscopy showed that myocardial injury was found in I/R group .With the prolongation of reperfusion time ,the degree of myocardial injury was gradually aggravated ,and the injury was alleviated after irradiation .Conclusions IL-8 monoclonal antibody combined with UTMD has the advantages of non-invasive and highly effective in alleviating MIRI .It provides a new way to treat MIRI .

19.
Article in Korean | WPRIM | ID: wpr-787348

ABSTRACT

Post-endodontic restorations are both important and challenging for clinical success in endodontically treated posterior teeth. Several options have been proposed to restore endodontically treated molars.In pediatric dentistry, restoration using conventional single crowns, especially for partially erupted molars with insufficient retentive tooth structure, has proven to be difficult. However, the endocrown presents a conservative and esthetic restorative alternative to conventional crowns with post-and-core, as it acquires additional retention within the pulp chamber. The tooth preparation consists of a circular, equigingival, butt-joint margin and a central retention cavity in the pulp chamber that helps to construct both the crown and core as a single unit.This case report describes the esthetic and conservative endocrown restorations of erupting permanent first molars with extensive coronal destruction.


Subject(s)
Crowns , Dental Pulp Cavity , Molar , Pediatric Dentistry , Tooth , Tooth Preparation
20.
Article in Chinese | WPRIM | ID: wpr-755688

ABSTRACT

Objective To investigate the therapeutic effect and mechanism of non-mitogenic acid fibroblast growth factor 1( NMFGF1) on diabetic cardiomyopathy ( DCM) by using PEG-modified nano-liposomes combined with ultrasound-targeted microbubble destruction technique ( UTMD ) . Methods The NMFGF1 loaded PEG-modified nano-liposomes were prepared by a water-in-water emulsion method and their quality inspections were also investigated. Type 1 diabetes animal model was induced by intraperitoneal injection of streptozotocin ( 70 mg/kg) in male SD rats. The diabetic rats were raised twelve weeks after the diabetes model was established and DCM rats were selected by ultrasonic heart function examination. After two weeks of intervention, all rats were kept for another two weeks and then underwent transthoracic echocardiography examination. The rats were sacrificed and myocardial tissue was obtained to quantify myocardial collagen fraction ( CVF ) and cardiac myocyte apoptotic index by Sirius red staining and TUNEL staining. Results NMFGF1-loaded PEG-nano-liposomes showed a good morphology and 90.3%± 1.4% NMFGF1 encapsulation efficiency. Compared with DCM group, NMFGF1group, and NMFGF1-PEG-nano-liposomes group, NMFGF1-loaded PEG-nano-liposome plus UTMD group showed increased left ventricular end diastolic diameter (LVIDd) [(7.36±0.42) vs (5.75±0.24), (6.64±0.27), (6.72±0.24)mm, all P<0.05]and leftventricularfractionshortening(LVFS) [(50±3) vs (33±2), (44±5), (43±3)mm, all P<0.05], and decreased left ventricular posterior wall thickness (LVPW) [(1.65±0.07) vs (1.89±0.08), (1.73±0.11), (1.73 ±0.07) mm, all P<0.05], with decreased CVF and apoptotic index(all P<0.05). Conclusion PEG-nano-liposomes combining with UTMD technique has a greater translational potential in the delivery of NMFGF1 for the treatment of DCM by attenuating oxidative stress-induced injury and may provide a promising strategy for treating diabetes cardiomyopathy.

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