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1.
Article in Chinese | WPRIM | ID: wpr-690005

ABSTRACT

<p><b>OBJECTIVE</b>To explore function and related molecular mechanism of osteopractic total flavone (OTF) on tendon healing in rats.</p><p><b>METHODS</b>Ten male rats aged for 8 weeks were collected and weighted from 180 to 220 g. Tendon stem cells were cultivated, the third tendon stem cells were used for experiment. OTP treated with 0, 0.1, 1, 10 ng/ml were added into tendon stem cells, and expression change of ALP, Runx2, OCN, VEGF, P-S6, P-4E/BP1 were detected after 14 days. Forty male rats aged for 8 weeks (weighted 180 to 220 g) were established extra-articular tendon-bone transplanting healing model, and divided into experimental group and control group. Experimental group were treated with OTF(100 mg·kg⁻¹·d⁻¹), while control group was treated by normal saline with the same volume. Tendon-bone healing degree were detected by biomechanical testing at 3 and 6 weeks after surgery, histological detection were applied to detect tendon-bone healing and number of new vessles.</p><p><b>RESULTS</b>After treated by OTP, ALP staining and active index detection showed there were statistical differences among 0, 0.1, 1, 10 ng/ml group. After 14 days' cultivation, western blotting results showed mTOR downstream marker protein P-S6 protein expression were gradually increased with increase of density of OTP, expression of P-4E/BP1 was reduced, while expression of Runx2, OCN, VEGF were increased. Biological detection results showed that there was no significant difference in mechanical strength between experimental group(0.78±0.05) N/mm and control group (0.51±0.02) N/mm at 3 weeks after surgery, while mechanical strength in experimental group (1.36±0.09) N/mm was higher than control group (1.01±0.08) N/mm at 6 weeks after surgery. Histological results showed maturity of tendon-bone surface cell were higher at 3 and 6 weeks in experimental group, sharpey fiber growth more density, calcification extent of mesenchyme was high, and new bone, vessels were increased.</p><p><b>CONCLUSIONS</b>OTF could promote osteogenic differentiation of tendon stem cells through mTOR signaling in vitro, and stimulate tendon-bone healing in bone tunnel and enhance connection quality between tendon and bone.</p>


Subject(s)
Animals , Biomechanical Phenomena , Bone Transplantation , Cell Differentiation , Cells, Cultured , Flavones , Pharmacology , Male , Osteogenesis , Rats , Stem Cells , Cell Biology , TOR Serine-Threonine Kinases , Metabolism , Tendons , Cell Biology , Transplantation , Wound Healing
2.
Chinese Pharmaceutical Journal ; (24): 1359-1365, 2018.
Article in Chinese | WPRIM | ID: wpr-858238

ABSTRACT

OBJECTIVE: To study the chemical constituents from the rhizome of Drynaria fortunei and the protective effects of them on PC12 cells induced by Aβ25-35. METHODS: The compounds were isolated and purified by silica gel, Sephadex LH-20, ODS column chromatography, and their structures were identified on basis of spectroscopic METHODS:, such as MS and NMR. PC12 cells were treated with Aβ25-35 to establish the Alzheimer' s disease models. The compounds of different concentrations were added into culture medium to detect the protection. MTT assay was used to detect cell vitality and to observe the protective effects of compounds on PC12 cells induced by Aβ25-35. RESULTS: Nine compounds were isolated and identified as naringin(1), neoeriocitrin(2), 5,7-dihydroxychromone-7-neohesperidoside(3), (E)-4-O-β-D-glucopyranosyl caffeic acid(4), kaempferol(5), luteolin(6), protocatechoic acid(7), psoralen(8), and β-sitosterol(9). The cell experiments were performed on the compounds 1-8 and the RESULTS: showed they can promote the proliferation of PC12 cells. The cell vitality increase with concentration rising, and the difference is statistically significant (P<0.05). CONCLUSION: Compounds 1-8 play an important role in protecting Aβ25-35-induced injury in PC12 cells and they are the main active components of Drynaria fortunei in the protection of central nervous function.

3.
Chinese Journal of Orthopaedics ; (12): 177-183, 2016.
Article in Chinese | WPRIM | ID: wpr-483451

ABSTRACT

Objective To explore the angiogenesis&neovascularization effects of naringin treatment in ovariectomized rats’fracture healing. Methods Upper 1/3 transverse tibial fracture model 4 weeks later after ovariectomized were estimated and randomly divided into the naringin group and control group. Microfil perfusion technique was used to analysis the angiogene?sis situation at two weeks after bone fracture. HE staining was used to evaluate the level of angiogenesis&neovascularization of tis?sue from histological point of view. The relative expression of VEGF in the callus was identified by real?time polymerase chain re?action. Immunohistochemical technique was used to observe the vessel endothelial growth factor?2 in the callus of the two groups. Maximum fracture load was tested by three?point bend test. Results The vascular volume and vascular density were more in nar?ingin group than control group. The HE staining of the 2 week group slices shows that the VA, VN2 of the unit of high magnifica?tion vision of the naringin group was significantly larger compared to the control group. Real?time PCR revealed that the compara?tive expression of VEGF is more in naringin group than in control group; the positive number of VEGFR?2 is more in naringin group than in control group. Naringin can promote the maximum load of the callus. Conclusion Naringin can promote ovariecto?mized rats’angiogenesis&neovascularization in the early process of fracture healing. It may be act on the signaling pathway of VEGF/VEGFR?2.

4.
Article in Chinese | WPRIM | ID: wpr-855698

ABSTRACT

Objective: To study the chemical constituents from the rhizome of Drynaria fortunei. Methods: Silica gel, ODS, Sephadex LH-20 column chromatography, and semi-preparative HPLC were used to isolate pure compounds. The compounds were identified on the basis of their physicochemical properties and spectroscopic data. Results: Fourteen compounds were isolated from the rhizome of D. fortunei, including three lignans (1-3) and eight flavonoids (4-11). By spectroscopic techniques, such as 1H-NMR, 13C-NMR, and ESI-MS, these compounds were identified as (7′R,8′S)-dihydrodehydrodiconiferyl alcohol 4′-O-β-D-glucopyranoside (1), lariciresinol 4′-O-β-D-glucopyranoside (2), (-)-secoisolariciresinol 4-O-β-D-glucopyranoside (3), eriodictyol (4), eriodictyol 7-O-β-D-glucopyranoside (5), neoeriocitrin (6), naringin (7), luteolin (8), luteolin 7-O-β-D-glucopyranoside (9), luteolin 8-C-β-D- glucopyranoside (10), 2′,4′-dihydroxydihydrochalcone (11), maltol 3-O-β-D-glucopyranoside (12), β-sitosterol (13), and daucosterol (14). Conclusion: Compounds 1-3, and 11 are isolated from the plants in Polypodiaceae family for the first time, and compounds 5 and 8-10 are isolated from the plants of Drynaria (Bory) J. Sm. for the first time. Compound 1 is present as rotamers at room temperature.

5.
Article in English | WPRIM | ID: wpr-148595

ABSTRACT

One of the traditional Korean medicine, Drynaria fortunei (D. fortunei) is one of candidates known to be effective for the treatment of inflammation, hyperlipemia, arteriosclerosis, rheumatism, and gynecological diseases such as osteoporosis and bone resorption. The present study investigated the antimicrobial activity of methanol (MeOH) extract and n-butanol (n-BuOH), chloroform (CHCl3), and ethyl acetate (EtOAc) fractions of D. fortunei against oral bacteria. The n-BuOH and CHCl3 fractions (MICs, 0.0078 to 0.3125 mg/ml; MBCs, 0.019 to 0.625 mg/ml) were demonstrated as strong antibacterial activity than the MeOH extract and EtOAc fraction. The combination effects of n-BuOH fraction with ampicillin or gentamicin were synergistic against some oral bacteria. We suggest that D. fortunei could be employed as a natural antibacterial agent in oral care products.


Subject(s)
1-Butanol , Ampicillin , Arteriosclerosis , Bacteria , Bone Resorption , Chloroform , Gentamicins , Hyperlipidemias , Inflammation , Methanol , Osteoporosis , Polypodiaceae , Rheumatic Diseases
6.
Pharmaceutical Journal ; : 18-20, 2003.
Article in Vietnamese | WPRIM | ID: wpr-6005

ABSTRACT

The acute toxicity and analgesic, sedative effects of Drynaria fortunei - Polypodiaceae were studied on experimental mice and determined LD50 by Litchfield-Wilcoxon method. Results: this drug in dosage of 4g/kg has analgesic and sedative effect. It hasn't acute toxicity in dosage 64-fold treating dosage


Subject(s)
Toxicity , Analgesics , Polypodiaceae , Medicine, Traditional
7.
Article in Vietnamese | WPRIM | ID: wpr-5375

ABSTRACT

The anti-flammatory effect of Drynaria fortunei was studied on rat’s hind paw edema induced by carrageenin. Results showed that its aqueous extract in the dose of 4g/kg body weight exerts anti-inflammatory effect. Acute effect of Drynaria fortunei is exhibited through the decreased quantity of exudation and the number of leucocyte in the exudation.


Subject(s)
Anti-Inflammatory Agents , Animal Experimentation , Medicine, Traditional
8.
Article in Chinese | WPRIM | ID: wpr-577951

ABSTRACT

Objective To establish a separation method for neoeriocitrin and naringin reference sub- stances from Drynaria fortunei.Methods The extract of D.fortunei was isolated and purified by macro porous resin,silica gel column chromatography,neutro-aluminum oxide adsorbing,Sephadex LH-20 co- lumn chromatography,and recrystallizations.The structures of neoeriocitrin and naringin were identified by MS,IR,UV,~1H-NMR,and ~(13)C-NMR.Results The contents of neoeriocitrin and naringin were 99.5% and 99.3%,respectively.Conclusion The developed method is simple with lower cost,by which neoeriocitrin and naringin can be used as reference substances for the qualitative and quantitative analysis of Chinese herbal medicine.

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