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1.
Braz. dent. j ; 33(3): 8-17, July-Sept. 2022. tab, graf
Article in English | LILACS-Express | LILACS, BBO | ID: biblio-1384037

ABSTRACT

Abstract This study evaluated the effect of photodynamic therapy (PDT) on infected root canals. Twenty-one human teeth were selected, and 18 were infected by E. faecalis for 60 days. The antimicrobial strategies tested were: G1. Root canal preparation (RCP) using Niquel-Titanium (NiTi) rotary instruments, 2.5% NaOCl, and final irrigation with 17% EDTA, followed by PDT with methylene blue photosensitizer and laser diode low power; G2. RCP using stainless steel files and the same irrigation and PDT protocols as G1; G3. Same RCP protocol as G1 without PDT; G4. Only irrigation with 2.5% NaOCl; G5. Same PDT protocol as G1 without RCP; G6. Negative control; G7. Positive control. Samples for microbiological tests were collected initially (S1), after RCP (S2), and after PDT (S3). Subsequently, the roots were sectioned and prepared for Scanning Electron Microscopy (SEM) analysis. Bacterial growth was analyzed according to the turbidity of the culture medium, followed by spectrophotometric optical density (nm). The effect of PDT on the dentinal structure was evaluated at magnifications 1,600X and 5,000X and described qualitatively. The Wilcoxon test was used for the comparisons from the same specimens, and the Mann-Whitney test was used to compare groups ((=5%). Bacteria were found in all experimental groups' microbiological samples (S1, S2 and S3). The optical density of culture media was lower in S2 than in S1 of G1, 2, 3, and 4 (p> 0.05). After PDT (S3) in G1 and 2, there was an additional reduction in optical density of the culture medium, respectively (p>0.05). In Group 5, the analysis of culture media at S2 revealed an increase in optical density compared to S1(p>0.05). In SEM images of G1, 2, and 5, dentin with melting and recrystallization areas were evidenced. After preparation of the root canal with the rotary system or manually associated with 2.5% NaOCl, PDT was not able to completely eliminate E. faecalis present in the root canal.


Resumo Este estudo avaliou o efeito da terapia fotodinâmica (PDT) em canais radiculares infectados com E. faecalis. Vinte e um dentes humanos extraídos foram selecionados, e 18 foram infectados por E. faecalis por 60 dias. As estratégias antimicrobianas testadas foram: G1. Preparo do canal radicular (PCR) com instrumentos rotatórios de NiTi, NaOCl 2,5% e irrigação final com EDTA 17%, seguido de PDT com fotossensibilizador azul de metileno e laser diodo de baixa potência; G2. PCR usando limas de aço inoxidável e os mesmos protocolos de irrigação e PDT do G1; G3. Protocolo de PCR similar que G1 sem PDT; G4. Somente irrigação com NaOCl 2,5%; G5. Protocolo similar ao G1, sem PCR; G6. Controle negativo; G7. Controle positivo. Amostras para exames microbiológicos foram coletadas inicialmente (S1), após PCR (S2) e após PDT (S3). Na sequência, as raízes foram seccionadas e preparadas para análise em microscopia eletrônica de varredura (MEV). O crescimento bacteriano foi analisado de acordo com a turbidez do meio de cultura seguida pela densidade óptica espectrofotométrica (nm). O efeito da PDT na estrutura dentinária foi avaliado em aumentos de 1.600X e 5.000X, e descrito qualitativamente. O teste de Wilcoxon foi utilizado para as comparações dos mesmos espécimes e o teste de Mann-Whitney para as comparações entre os grupos ((=5%). Bactérias foram encontradas em todos os grupos experimentais, e em todas as coletas microbiológicas (S1, S2 e S3). A densidade óptica dos meios de cultura foi menor em S2 do que em S1 de G1, 2, 3 e 4 (p>0,05). Após a PDT (S3) em G1 e 2, houve redução adicional na densidade óptica do meio de cultura de 90,0% e 92,0%, respectivamente (p>0,05). No Grupo 5, a análise dos meios de cultura em S2 revelou um aumento de 3,2% na densidade óptica em comparação com S1(p>0,05). Nas imagens de MEV do G1, 2 e 5 foram evidenciadas dentina com áreas de fusão e recristalização. O PDT utilizado após preparo do canal radicular com sistema rotatório ou manual, associado ao NaOCl 2,5%, não foi capaz de eliminar completamente o E. faecalis em biofilme maduro presente no canal radicular.

2.
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1385863

ABSTRACT

RESUMEN: El objetivo del estudio experimental in vitro fue evaluar la eficacia antimicrobiana del cemento Portland (CP) comparado con el Mineral Trióxido Agregado (MTA) frente a cepas de Enterococcus faecalis (E. faecalis) y Candida albicans (C. albicans) a las 24, 48 y 72 horas después del sembrado. Para ello se utilizó la técnica de difusión en agar. La capa base fue realizada con el Agar Müller-Hinton en el que se hicieron agujeros de 6 mm de diámetro a fin de contener los cementos de estudio y medir posteriormente la zona de inhibición microbiana alrededor de los materiales mediante un calibrador vernier digital. Se utilizó la prueba T de Student para comparar la diferencia entre el CP y MTA y el análisis de ANOVA para evaluar las diferencias entre los tres tiempos de estudio. Los resultados mostraron que tanto frente a E. faecalis y C. albicans el CP tuvo un mayor halo de inhibición que el MTA, siendo estas diferencias estadísticamente significativas (p0.05). Asimismo, la C. albicans presentó valores mayores de inhibición que el E. faecalis en los dos cementos estudiados. Se concluyó que el CP tuvo mayor eficacia antimicrobiana que el MTA independientemente del tiempo evaluado.


ABSTRACT: The aim of the experimental in vitro study was to evaluate the antimicrobial efficacy of Portland cement (CP) compared with Mineral Trioxide Aggregate (MTA) against Enterococcus faecalis (E. faecalis) and Candida albicans (C. albicans) strains at 24, 48 and 72 hours after sowing. For this, the agar diffusion technique was used. The base layer was made with Müller-Hinton Agar in which 6 mm diameter holes were made in order to contain the study cements and subsequently measure the zone of microbial inhibition around the materials using a digital vernier caliper. The Student's t test was used to compare the difference between CP and MTA and the ANOVA analysis to evaluate the differences between the three study times. The results showed that both against E. faecalis and C. albicans, CP had a greater inhibition than MTA, these differences being statistically significant (p 0.05). Likewise, C. albicans presented higher inhibition values than E. faecalis in the two cements studied. It was concluded that CP had greater antimicrobial efficacy than MTA regardless of the time evaluated.

3.
Braz. dent. j ; 33(1): 96-104, jan.-fev. 2022. tab, graf
Article in English | LILACS-Express | LILACS, BBO | ID: biblio-1364485

ABSTRACT

Abstract Bacteria are related do different oral diseases, such as dental caries and periodontal disease. Therefore, the control or/and eradication of microorganisms and their by-products is primordial for the success of their treatment. An alternative for decrease bacterial load is the use of plant extracts used in popular medicine. The cytotoxicity and antimicrobial action of extracts of Cariniana rubra Gardiner ex Miers, Senna martiniana, Anadenanthera colubrina (Vell.) Brenan and Spiranthera odoratissima St. Hil. against strains of Streptococcus mutans, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Aggregatibacter actinomyces- tencomitans and Candida albicans were investigated. Cytotoxicity was assessed at concentrations of 1, 10, 40, 80, 100 and 1000 μg/mL by means of the MTT test and compared to a control group with untreated cells. Those with acceptable cytotoxicity had the antimicrobial action measured by the XTT test. As a positive control, sodium hypochlorite was used. Cariniana rubra Gardiner ex Miers had the highest citototoxicity results while Spiranthera odoratissima St. Hil. had the best results, but all extracts showed acceptable cytotoxicity at different concentrations. The plant extracts showed higher activity against A. actinomycetencomitans: Anadenanthera columbrina (Vell.) Brenan (80.52%) at 40 μg/mL, Spiranthera odoratissima St. Hil (78.48%) in 1 μg/mL, Senna martiniana (73.28%) in the concentration of 40 μg/mL and Cariniana rubra Gardiner ex Miers (70.50%) in 10 μg/mL. All extracts analyzed showed acceptable cytotoxicity at different concentrations and were promising for inhibition of the pathogenic microorganisms studied.


Resumo Bactérias estão relacionadas a diferentes doenças bucais, como a cárie dentária e a doença periodontal. Assim, o controle e/ou erradicação de microrganismos e seus subprodutos é primordial para o sucesso dos tratamentos. Uma alternativa para diminuir a carga bacteriana é a utilização de extratos vegetais utilizados na medicina popular. A citotoxicidade e ação antimicrobiana de extratos de Cariniana rubra Gardinerex Miers, Senna martiniana H.S. Irwin & Barneby, Anadenanthera colubrina (Vell.) Brenan e Spiranthera odoratissima St. Hil. contra cepas de Streptococcus mutans, Enterococcusfaecalis, Staphylococcus aureus, Escherichia coli, Agartibacter actinomycetencomitans e Candida albicans foram investigados. A citotoxicidade foi avaliada nas concentrações de 1, 10, 40, 80, 100 e 1000 μg/mL por meio do teste MTT. Aqueles com citotoxicidade aceitável tiveram a ação antimicrobiana medida pelo teste XTT. Cariniana rubra Gardinerex Miers apresentou os maiores resultados de citototoxicidade, enquanto Spiranthera odoratissima St. Hil. obteve os melhores resultados, mas todos os extratos apresentaram citotoxicidade aceitável em diferentes concentrações. Os extratos vegetais apresentaram maior atividade contra A. actinomycetencomitans: Anadenanthera columbrina (Vell.) Brenan (80,52%) a 40 μg/mL, Spiranthera odoratissima St. Hil (78,48%) em 1 μg/mL, Senna martiniana H.S. Irwin & Barneby (73,28%) na concentração de 40 μg/mL e Cariniana rubra Gardinerex Miers (70,50%) em 10 μg/mL. Todos os extratos analisados apresentaram citotoxicidade aceitável em diferentes concentrações e foram promissores na inibição dos microrganismos patogênicos estudados.

4.
repert. med. cir ; 31(1): 63-70, 2022. tab.
Article in English, Spanish | LILACS, COLNAL | ID: biblio-1366995

ABSTRACT

Introducción: los enterococos son responsables de múltiples infecciones y por su creciente patrón de resistencia se ha vuelto de interés en el país y en el mundo. Objetivo: caracterizar las infecciones por Enterococcus spp. Metodología: estudio descriptivo, retrospectivo observacional transversal desde enero 2015 hasta enero 2018 en un hospital regional. Resultados: la prevalencia de las infecciones por Enterococcus spp. fue de 0,154%. El E. faecalis fue el más aislado, seguido del E. faecium. La resistencia a ampicilina fue de 19% y a vancomicina de 10%; 32% de los pacientes tuvieron terapia empírica con vancomicina y 22% con piperacilina tazobactam, la mediana de antibioticoterapia fue de 10 días. Discusión: el interés por los Enterococcus spp. se ha incrementado debido a que representan una carga importante en las infecciones asociadas con la atención en salud (IAAS). La mayoría se dan en hombres con una edad mediana de 40 a 60 años, hospitalizados en UCI, con infecciones urinarias y comorbilidades como inmunosupresión y cirugías previas. Conclusión: como ha venido reportándose aumento en las tasas de resistencia a vancomicina y ampicilina, se recomienda el uso responsable de la terapia antibiótica, con la finalidad de erradicar en forma eficaz al patógeno y prevenir nuevas resistencias.


Introduction: enterococci can cause multiple infections and due to their increasing resistance to antibiotics they have become of national and global concern. Objective: to characterize infections caused by Enterococcus spp.Methodology: descriptive, retrospective observational cross-sectional study conducted from January 2015 to January 2018 in a regional hospital. Results: the prevalence of Enterococcus spp. infections was 0.154%. E. faecalis was the most commonly isolated, followed by E. faecium. Antibiotic resistance was 19% and 10% for ampicillin and vancomycin respectively, 32% of patients received empirical therapy with vancomycin and 22% with piperacillin tazobactam. The median duration of antibiotic therapy was 10 days. Discussion: interest in Enterococcus spp. has increased for they are recognized as an important burden in healthcare-associated infections (HAIs). Enterococcal infection occurs mainly among men, median age 40 to 60 years, hospitalized in the ICU, with urinary tract infections and comorbidities such as immunosuppression and previous surgeries. Conclusion: as an increased rate of vancomycin and ampicillin resistance in enterococci has been reported, a responsible use of antibiotic therapy is recommended in order to effectively eradicate the pathogen and prevent the emergence of new bacterial resistances.


Subject(s)
Humans , Male , Female , Middle Aged , Drug Resistance, Microbial , Epidemiology , Enterococcus , Delivery of Health Care , Hospitals
5.
Braz. arch. biol. technol ; 65: e22210091, 2022. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1364453

ABSTRACT

Abstract: The aim of this research was to determine the probiotic potential and safety of lactic acid bacteria (LAB) isolated from raw goat milk. Gram positive and catalase negative bacteria were isolated from raw goat milk (n = 61) and identified as LAB. LAB isolates were screened for antimicrobial, probiotic and technological characteristics. LAB isolates showed antimicrobial activity against foodborne pathogens (Staphylococcus aureus, Escherichia coli and Salmonella Typhimurium) and high survival rate at pH 2 (93.54-100.38% after 4h), in the presence of 0.3% bile salts (100.85-108.96% after 4h) and simulated gastric fluid (74.16-80.13% after 3h). Three LAB isolates (1, 3 and 13) with high antimicrobial activity against all foodborne pathogens and probiotics characteristics were subjected to 16S rRNA sequencing and identified as Enterococcus faecium strains. Enterococcus spp. exhibited milk coagulation potential, amylolytic activity, susceptibility to antibiotics and no evidence of hemolysis. Enterococcus spp. isolated from goat milk showed probiotic and technological characteristics and can be used as a starter culture after further safety evaluation.

6.
J. appl. oral sci ; 30: e20210575, 2022. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1365010

ABSTRACT

Abstract Objective: To assess the effects of different peracetic acid (PAA) formulations on smear layer (SL) removal, dentine erosion, cytotoxicity, and antibiofilm activity. Methodology: SL removal and dentine erosion were assessed using 90 premolars, distributed into six groups, according to final irrigation: PAA formulations (1% Sigma, 1% Bacterend OX, 1% Arposept, and 0.09-0.15% Anioxyde), 17% ethylenediaminetetraacetic acid (EDTA), and water (control). Cytotoxicity was assessed by methyl-thiazol-tetrazolium (MTT) and neutral red assays. Antibacterial and antibiofilm effectiveness was evaluated against Enterococcus faecalis. For cytotoxicity and antibiofilm activity assessment, the 2.5% NaOCl was also included. Results: EDTA, Sigma, and Bacterend OX removed more SL than Arposept, Anioxyde, and water (p<0.05). EDTA caused more severe dentine erosion than Sigma and Bacterend OX (p<0.05). Sigma and Bacterend OX had higher cytotoxicity than the other solutions (p<0.05). NaOCl, Bacterend OX, Sigma, and Anioxyde significantly reduced E. faecalis colony-forming units (CFU) (p<0.05). The 2.5% NaOCl solution promoted greater biofilm biomass reduction (p<0.05) than the other solutions. All PAA formulations promoted greater biomass reduction than 17% EDTA (p<0.05). Conclusions: Although Sigma and Bacterend OX had higher cytotoxicity, they had a SL removal capability similar to that of EDTA, were as effective as NaOCl against E. faecalis biofilm, and promoted less dentine erosion than EDTA. Arposept and Anioxyde failed to remove the SL, had lower cytotoxicity, and showed less bacterial activity than NaOCl.

7.
Article in Chinese | WPRIM | ID: wpr-904716

ABSTRACT

Objective@#To study the distribution of pathogenic infection and relevance in combined periodontal-endodontic lesions of periodontal origin,and provide the basis for clinical treatment. @*Methods@#Totally 43 cases of combined periodontal-endodontic lesions of periodontal origin from Jan. 2018 to Jun. 2020 treated in the hospital were selected, including a total of 43 teeth as the experimental group. Another 41 teeth from 41 cases with severe periodontitis during the same period were set as the control group. subgingival plaque samples and root canal samples of ill teeth were made for test. Quantitative Real-time PCR was used to detect the quantity of Tannerella forsythia (Tf), Porphyromonas gingivalis (Pg), Fusobacterium nucleatum (Fn), Prevotella intermedia (Pi), Treponema denticola (Td), Digestive streptococcus (Ds), Enterococcus faecalis (Ef), Porphyromanus endodontics (Pe). @*Results@# There was no significant difference in the quantity of Digestive streptococcus and Porphyromanus endodontics in the root canal tissue and subgingival plaque of the experimental group (Ρ>0.05), other six pathogenic bacteria in the subgingival plaque samples was significantly higher than that from the root canal tissue (P<0.05); No significant difference in the quantity of Digestive streptococcus was found in the subgingival plaque between the two groups (P=0.241). Other seven pathogenic bacteria in the subgingival plaque samples of the experimental group was significantly higher than that from the control group (P<0.05); The number of Ef, Pe, Pg, Td and Tf in the root canal tissue was closely related to the subgingival plaque in the experimental group, Ef (r=0.347, Ρ < 0.05), Pe (r=0.363, Ρ < 0.05), Pg (r=0.437, Ρ < 0.01), Td (r=0.471, Ρ < 0.01), Tf (r=0.679, Ρ < 0.01).@*Conclusion @# The quantity of common pathogenic bacteria in the root canal tissue of Combined periodontaI-endodontic lesions of periodontal origin was lower than that from the subgingival plaque sample, and the quantity of common pathogenic bacteria in the root canal tissue was closely related to the subgingival plaque. During clinical treatment, attention should be paid to the control of pulp tissue infection while controlling periodontal tissue infection.

8.
Organ Transplantation ; (6): 225-2022.
Article in Chinese | WPRIM | ID: wpr-920853

ABSTRACT

Objective To analyze the distribution characteristics and drug resistance of pathogens in infected donors from organ donation after citizen's death. Methods Clinical data of 465 potential donors from organ donation after citizen's death were retrospectively analyzed. The airway secretion, urine and blood samples of all donors were cultured. The infection rate of the donors, the source and composition ratio of pathogens were summarized. The drug resistance of main Gram-negative and Gram-positive pathogens was analyzed. Results Among 465 donors, 330 cases were infected and the infection rate was 71.0%. Among the positive culture samples of all donors, lower respiratory tract samples accounted for 63.8%(292/458), 18.6%(85/458) for blood samples and 17.7%(81/458) for urine samples. A total of 512 pathogens were isolated, including 75.0%(384/512) of Gram-negative pathogens, 18.2%(93/512) of Gram-positive pathogens followed by 6.8%(35/512) of fungi. Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii were the most common Gram-negative pathogens. Klebsiella pneumoniae was sensitive to quinolones, compound preparations containing β-lactamase inhibitor (piperacillin-tazobactam, cefoperazone sodium-sulbactam sodium) and carbapenem antibiotics, whereas less sensitive to cephalosporins. Pseudomonas aeruginosa was sensitive to β-lactams, quinolones and aminoglycosides. Acinetobacter baumannii was sensitive to polymyxin, tigecycline and amikacin, whereas resistant to the other antibiotics. No Gram-positive pathogens was resistant to vancomycin, linezolid and teicoplanin. Staphylococcus aureus and coagulase-negative staphylococci were the most commonly isolated Gram-positive pathogens, which yielded resistance rates of 36% and 87% to oxacillin sodium, and were generally resistant to penicillin and erythromycin. The resistance rate of Enterococcus faecalis to quinolones and erythromycin exceeded 90%, and 55% for high-concentration gentamicin. Conclusions The infection rate of organ donors from organ donation after citizen's death is relatively high, and the main infection site is lung. Gram-negative pathogens are the most commonly isolated strains, and certain strains tend to exhibit multiple drug resistance.

9.
Rev. Soc. Bras. Med. Trop ; 55: e0353, 2022. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1394694

ABSTRACT

ABSTRACT Background: Domestic pigeons carry pathogens in their droppings, posing a potential public health problem. Methods: The phenotypic and genotypic antimicrobial resistances of Staphylococcus aureus and Enterococcus faecium in the feces of urban pigeons near hospitals with intensive care units were measured. Results: Twenty-nine samples showed Enterococcus growth, whereas one was positive for S. aureus. The S. aureus isolate was sensitive to the antibiotics tested via antibiogram, however resistance genes were identified. E. faecium isolates showed phenotypic resistance to gentamicin, erythromycin, and ciprofloxacin. Conclusions: Antimicrobial profiles harmful to health were demonstrated in bacterial pathogens isolated from the external environment of hospitals.

10.
Braz. j. biol ; 81(4): 954-961, Oct.-Dec. 2021. tab, graf
Article in English | LILACS | ID: biblio-1153438

ABSTRACT

Abstract The aim of present study is to characterize the resistance and virulence profile of enterococci isolated from aquaculture excavated ponds and masonry tanks (6 samples) in southern Brazil. Samples were cultured in selective medium, 10 colonies were randomly selected from each sample, which were identified by MALDI-TOF and tested against 13 antimicrobials. The presence of resistance (tetL, tetM, tetS, ermB and msrC) and virulence (ace, esp, agg, cylA and gelE) genes were determined by PCR. A total of 79 enterococci were identified, and Entecococcus faecalis (44.3%) and E. casseliflavus (36.7%) were the most prevalent species isolated. Sixty-five strains (82.3%) were resistant to at least one of the antimicrobials tested, whereas 27 (34.2%) strains were multiresistant. The overall percentages of antimicrobial resistant isolates were: 58.2% to rifampicin, 40.5% to fluoroquinolones, 36.7% to erythromycin and 30.4% to tetracycline. The tetL and tetM genes were found in 57.7% of the tetracycline-resistant strains; and msrC in 31.01% of erythromycin-resistant strains. The most frequently detected virulence factors were ace and gelE genes. Although limited to a single farm, these data suggest that aquaculture may be a reservoir of resistant and virulent enterococci. This study is the first step towards enhancing our understandingof distribution, resistance and virulence profile in enterococci isolated from fish farming environments in the south Brazil.


Resumo O objetivo do estudo apresentado é caracterizar o perfil de resistência e virulência de enterococos isolados de viveiros escavados e tanques de alvenaria (6 amostras) de uma pisicultura no Sul do Brasil. As amostras foram cultivadas em meio seletivo, 10 colônias foram selecionadas aleatoriamente de cada amostra, que foram identificadas por MALDI-TOF e testadas contra 13 antimicrobianos. A presença de genes de resistência (tetL, tetM, tetS, ermB e msrC) e virulência (ace, esp, agg, cylA e gelE) foi determinada por PCR. Foram identificados 79 enterococos, sendo Entecococcus faecalis (44,3%) e E. casseliflavus (36,7%) as espécies mais frequentes isoladas. Sessenta e cinco cepas (82,3%) eram resistentes a pelo menos um dos antimicrobianos testados, enquanto 27 (34,2%) eram multirresistentes. As porcentagens gerais de isolados resistentes a antimicrobianos foram: 58,2% para rifampicina, 40,5% para fluoroquinolonas, 36,7% para eritromicina e 30,4% para tetraciclina. Os genes tetL e tetM foram encontrados em 57,7% das cepas resistentes à tetraciclina; e msrC em 31,01% das cepas resistentes à eritromicina. Os fatores de virulência mais comumente detectados foram ace e gelE. Embora limitados a uma única fazenda, esses dados indicam que a aquicultura pode ser uma fonte de enterococos resistentes e virulentos. Este estudo é o primeiro passo para melhorar nosso entendimento da distribuição, resistência e perfil de virulência em enterococos isolados de ambientes de piscicultura no sul do Brasil.


Subject(s)
Animals , Enterococcus/genetics , Drug Resistance, Bacterial/genetics , Brazil , Microbial Sensitivity Tests , Agriculture
12.
Odontol. sanmarquina (Impr.) ; 24(4): 357-363, oct.-dic. 2021.
Article in Spanish, English | LILACS-Express | LILACS | ID: biblio-1342087

ABSTRACT

El objetivo de este estudio es conocer la efectividad del gel de clorhexidina y pasta de hidróxido de calcio con clorhexidina como medicamento intracanal en la eliminación del Enterococcus faecalis (E. faecalis). Se realizó una revisión bibliográfica mediante una búsqueda exhaustiva en diferentes bases de datos como: PubMed, ScienceDirect, SciELO, Springer, Google Scholar. Se seleccionaron mediante los criterios de inclusión y exclusión 37 artículos publicados en el periodo 2003-2020, los cuales fueron enfocados especialmente en E. faecalis y medicación intracanal. Teniendo como resultados que la clorhexidina en mayor porcentaje resultó ser el medicamento intracanal más eficaz en un tiempo promedio de 7 días, seguida de la combinación de hidróxido de calcio más clorhexidina que actuó de igual manera eliminando el E. faecalis de los canales radiculares, mientras que el hidróxido de calcio no presentó actividad antimicrobiana frente al E. faecalis.


The aim of this study was to know the effectiveness of chlorhexidine gel and calcium hydroxide paste with chlorhexidine as intracanal medicaments in Enterococcus faecalis(E. faecalis) elimination. A bibliographic review was done through an exhaustive search in different databases such as: PubMed, ScienceDirect, Scielo, Springer, Google Scholar. Using inclusion and exclusion criteria, 37 articles published in the period 2003-2020 were selected, which were especially focused on E. faecalis and intracanal medication. The analysis showed that chlorhexidine in a higher percentage was the most effective intracanal medication in an average time of 7 days, followed by the combination of calcium hydroxide plus chlorhexidine that acted in the same way eliminating E. faecalisfrom the root canals, while calcium hydroxide did not show any antimicrobial activity against E. faecalis.

13.
J. res. dent ; 9(5): 1-6, sep.-oct2021.
Article in English | LILACS-Express | LILACS | ID: biblio-1362910

ABSTRACT

Introduction: The methods used to evaluate the apical sealing of root canal filling materials have some limitations and great variability of results can be observed. Aim: The aim of this study was to compare, in an ex vivo apexification model, the results from bacterial and glucose leakage tests, which were applied in mineral trioxide aggregate (MTA) apical plugs. Materials and Methods: Sixty root segments (12mm) were randomly divided into 2 experimental groups (n=30): G1) MTA; G2) MTA + phosphate-buffered saline intracanal. Half of the specimens in each group were submitted to bacterial leakage test with E. faecalis for 70 days. The other half was submitted to the glucose leakage test under pressure (103KPa) for 60 min. The results from the two tests were compared based on the number of specimens presenting leakage. Data were analyzed by Fisher's test (p < 0.05). Results: There was no significant difference between tests for both groups analyzed (p > 0.05).Conclusion: The results of the present ex vivo study demonstrated that there was no difference between glucose and bacteria leakage evaluation methods, within the parameters of the present study and regardless

14.
Rev. colomb. cardiol ; 28(5): 483-488, sep.-oct. 2021. graf
Article in English | LILACS-Express | LILACS | ID: biblio-1357217

ABSTRACT

Abstract Infective endocarditis (IE) is a rare but severe disease, due to an infection of the endocardial surface of the heart. The annual incidence ranging from 3 to 7 per 100 000 person-years, with an overall mortality rates of 25%. Staphylococci and Streptococci accounted for approximately 80% of IE cases. Enterococci are the third leading cause accounted for approximately 5-18% of all cases and are increasingly linked to health-care contact. The increasing number of cardiovascular electronic devices, prosthetic valves implants along with frequent invasive diagnostic or therapeutic procedures performed in elderly, may also contribute to the rise of IE in this population. Blood cultures and echocardiographic findings are the cornerstone of the IE diagnosis, confirmed or rejected according to modified Duke criteria. Other imaging modalities as Cardiac Computed Tomography, Cardiac Magnetic Resonance Imaging and 18Ffluorodeoxyglucose Positron Emission Tomography - Computed Tomography (18FDG-PET/CT) can determine a more correct diagnosis and identify many of the endocarditis-related complications. Here, we describe a patient with aortic prosthetic valve and Enterococcus faecalis (EF) IE. Cerebral and spinal MRI and 18FDG-PET/CT, performed during the hospitalization, showed multi-organ silent periferic embolization. Furthermore, the cultural examination of the valvular surgical specimen revealed a methicillin-sensitive Staphylococcus aureus suggestive for polymicrobial endocarditis. Some Authors demonstrated a possible false-positive valve culture due to a postoperative contamination. Since contaminated cultures usually yield microorganisms compatible with endocarditis, such as coagulase-negative Staphylococci, viridans group Streptococcus species, may be difficult for the physician not to treat the patient. This case represents need for high level of suspicion to diagnose IE. Multimodality assessment improves the diagnosis and allows the detection of the complications. Moreover, a multidisciplinary team and specialized centers determine a better patient’s outcome.


Resumen La endocarditis infecciosa (EI) es una enfermedad poco frecuente pero severa, dada por una infección del endocardio. La incidencia anual oscila entre 3 y 7 por cada 100 000 personas-año, con una tasa de mortalidad general del 25%. Los estafilococos y los estreptococos representaron aproximadamente un 80% de los casos de EI. Los enterococos son la tercera causa, aportando aproximadamente 5% a 18% de todos los casos, y se vinculan cada vez más al contacto con la atención médica. El número creciente de dispositivos electrónicos cardiovasculares e implantes de válvulas protésicas, junto con los frecuentes procedimientos invasivos diagnósticos o terapéuticos en las personas ancianas, también podrían contribuir al incremento de EI en esta población. Los hemocultivos y los hallazgos ecocardiográficos son la piedra angular del diagnóstico de EI, confirmado o rechazado de acuerdo con los criterios modificados de Duke. Otras modalidades de imagenología tales como la Tomografía Computarizada Cardíaca (TCC), la Resonancia Magnética Cardíaca (RMC) y la Tomografía por Emisión de Positrones - Tomografía Computarizada con 18F-fluorodeoxiglucosa (18F-FDG PET/TC) pueden determinar un diagnóstico más preciso e identificar muchas de las complicaciones asociadas a la endocarditis. Aquí describimos un paciente con una válvula aórtica protésica y EI por EF. Una RM del cerebro y la columna vertebral y una 18F-FDG PET/TC practicadas durante la hospitalización evidenciaron embolia periférica multiorgánica silenciosa. Además, el examen por cultivo de la muestra quirúrgica valvular reveló Staphylococcus aureus sensible a la meticilina (SASM), sugestivo de endocarditis polimicrobiana. Algunos autores demostraron un posible falso positivo del cultivo valvular dado por contaminación postoperatoria. Ya que los cultivos contaminados generalmente producen microorganismos compatibles con la endocarditis, tales como estafilococos coagulasa negativos y estreptococos del grupo viridans, le puede resultar difícil al médico no tratar al paciente. Este caso representa la necesidad de tener un alto nivel de sospecha para diagnosticar la EI. La valoración multimodal mejora el diagnóstico y permite detectar complicaciones. Además, un equipo multidisciplinario y los centros especializados determinan un mejor desenlace para el paciente.

15.
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1385781

ABSTRACT

RESUMEN: El objetivo del estudio fue evaluar el efecto antimicrobiano de tres cementos selladores endodónticos frente a Streptococcus mutans, Enterococcus faecalis y Candida albicans. Se usó el método de difusión en agar, realizando pozos de 5 mm de diámetro por 4 mm de profundidad, en donde se colocaron los diferentes cementos que fueron preparados según las indicaciones del fabricante. Para S. mutans y E. faecalis se empleó agar cerebro-corazón, mientras que para C. albicans se empleó agar tripticasa soya como medios de cultivo. Las placas fueron incubadas por 48 horas a 37 ºC. Los halos de inhibición de crecimiento microbiano se midieron con un compás Vernier. Los resultados mostraron diferencias significativas entre todos los grupos p 0,05), siendo bastante inferior para E. faecalis (6,642 ? 0,659 mm) y C. albicans (8,781 ? 0,735 mm). El cemento a base de hidróxido de calcio (Sealer 26) fue más efectivo frente a S. mutans (13,010 ? 2,006 mm) que ante E. faecalis (6,165 ? 0,978 mm) y C. albicans (5,640 ? 0,280 mm). Como conclusión, el cemento a base de óxido de zinc y eugenol (Endofill) mostró el mejor efecto antimicrobiano contra las tres cepas, seguido de los cementos a base de resina (Vioseal) y finalmente hidróxido de calcio (Sealer 26).


ABSTRACT: The objective of the study was to evaluate the antimicrobial effect of three endodontic sealant cements for Streptococcus mutans, Enterococcus faecalis and Candida albicans. The agar diffusion method was used, making wells 5mm in diameter by 4mm deep, where the different cements that were prepared according to the manufacturer's instructions were placed. For S. mutans and E. faecalis, brain-heart agar was used, while for C. albicans trypticase soy agar was used as culture media. The plates were incubated for 48 hours at 37 ºC. The microbial growth inhibition halos were measured with a Vernier compass. The results showed significant differences between all groups p0.05), being considerably lower for E. faecalis (6.642 ? 0.659 mm) and C. albicans (8.781 ? 0.735 mm). Calcium hydroxide-based cement (Sealer 26) was more effective against S. mutans (13.010 ? 2.006 mm) than against E. faecalis (6.165 ? 0.978 mm) and C. albicans (5.640 ? 0.280 mm). In conclusion, cement based on zinc oxide and eugenol (Endofill) showed the best antimicrobial effect against the three strains, followed by resin-based cements (Vioseal) and finally calcium hydroxide (Sealer 26).

16.
Braz. dent. j ; 32(3): 1-9, May-June 2021. tab, graf
Article in English | LILACS, BBO | ID: biblio-1345506

ABSTRACT

Abstract Decontamination of the root canal (RC) system is essential for successful endodontic therapy. In this in vitro study, it was evaluated the disinfection potential of an electrofulguration device, the Endox® Endodontic System (EES), in RC infected with Enterococcus faecalis. Sixty-five human lower premolars were instrumented with MTwo® system. The specimens were distributed into six experimental groups (n = 10), according to the irrigation protocol: 2% chlorhexidine gel (CHX); CHX + EES; 5.25% sodium hypochlorite (NaOCl); NaOCl + EES; saline solution (SS); and SS + EES. Five specimens were untreated (control). RC samples were collected before (C1) and after EES treatment (C2), after chemo-mechanical preparation (CMP) (C3), and after final EES treatment (C4). All samples were plated for colony forming units (CFU/mL) onto solid media. The data were analyzed by Wilcoxon and Friedman tests for intragroup comparisons and by Kruskal Wallis test followed by Dunn's test for intergroup comparisons (α = 0.05). Treatment with the EES did not significantly reduce the number of CFU/mL as compared to baseline levels (C1 vs. C2, p> 0.05). After CMP (C3), all groups showed a significantly reduced amount of CFU/mL (p <0.05), with no difference between CHX- and NaOCl-treated samples (p >0.05). Lastly, treatment with the EES following CMP (C4) did not significantly reduce the amount of CFU/mL (C3 vs. C4, p> 0.05). To conclude, the use of the Endox® Endodontic System did not result in considerable bacterial reduction at all operative times, while treatment with NaOCl and CHX was equally efficient for this purpose.


Resumo A descontaminação do sistema do canal radicular (CR) é essencial para o sucesso da terapia endodôntica. Neste estudo in vitro, foi avaliado o potencial de desinfecção de um dispositivo de eletrofulguração, o Endox® Endodontic System (EES), em CR infectado com Enterococcus faecalis. Sessenta e cinco pré-molares inferiores humanos foram instrumentados com o sistema MTwo®. As amostras foram distribuídas em seis grupos experimentais (n = 10), de acordo com o protocolo de irrigação: clorexidina gel a 2% (CHX); CHX + EES; Hipoclorito de sódio 5,25% (NaOCl); NaOCl + EES; solução salina (SS); e SS + EES. Cinco amostras não foram tratadas (controle). As amostras de CR foram coletadas antes (C1) e após o tratamento com EES (C2), após preparo químico-mecânico (PQM) (C3) e após o tratamento final com EES (C4). Todas as amostras foram plaqueadas para unidades formadoras de colônias (CFU / mL) em meio sólido. Os dados foram analisados pelos testes de Wilcoxon e Friedman para comparações intragrupos e pelo teste de Kruskal Wallis, seguidos pelo teste de Dunn para comparações intergrupos (α = 0,05). O tratamento com o EES não reduziu significativamente o número de CFU / mL em comparação com os níveis basais (C1 vs. C2, p> 0,05). Após PQM (C3), todos os grupos apresentaram uma quantidade significativamente reduzida de CFU / mL (p <0,05), sem diferença entre as amostras tratadas com CHX e NaOCl (p> 0,05). Por fim, o tratamento com o EES após PQM (C4) não reduziu significativamente a quantidade de CFU / mL (C3 vs. C4, p> 0,05). Concluindo, o uso do Endox® Endodontic System não resultou em redução bacteriana considerável em todos os momentos operatórios, enquanto os tratamentos com NaOCl e CHX foram igualmente eficientes para esse fim.


Subject(s)
Humans , Root Canal Irrigants , Dental Pulp Cavity , Sodium Hypochlorite , Chlorhexidine , Enterococcus faecalis , Root Canal Preparation
17.
Braz. dent. j ; 32(3): 10-20, May-June 2021. tab, graf
Article in English | LILACS, BBO | ID: biblio-1345504

ABSTRACT

Abstract The host defense response to microbial challenge emerging from the root canal system leads to apical periodontitis. The aim of this study was to evaluate the expression of inflammatory cytokines and Nitric Oxide (NO) by macrophages after interaction with Enterococcus faecalis in the: plankton and dislodged biofilm mode; intact biofilm mode stimulated by calcium hydroxide (CH), CH and chlorhexidine (CHX) or Triple Antibiotic Paste (TAP). For this purpose, culture of macrophages from monocytes in human peripheral blood (N=8) were exposed to the different modes of bacteria for 24 hours. Subsequently, the cytokines, such as, Tumor Necrotic Factor- alfa (TNF-α), interleukin (IL)-1β, IL-6, IL-10; and NO were quantified by Luminex xMAP and Greiss reaction, respectively. In addition to the potential therapeutic effects of the intracanal medication, their antimicrobial activity against Enterococcus faecalis biofilm were also tested in vitro by confocal microscopy. The experiments` data were analyzed by the Kruskal-Wallis test with the Dunn post hoc test (α < 0.05). Bacteria in dislodged biofilm mode were shown to be more aggressive to the immune system than bacteria in plankton mode and negative control, inducing greater expression of NO and TNF-α. Relative to bacteria in intact biofilm mode, the weakest antimicrobial activity occurred in Group CH. In Groups CH/CHX and TAP the percentage of dead bacteria was significantly increased to the same extent. Interestingly, the biofilm itself did not induce the release of pro-inflammatory cytokines - except for NO - while the biofilm treated with TAP and CH based pastes enhanced the levels of IL-6 and TNF-α; and IL-1 β, respectively. In contrast, the levels of a potent anti-inflammatory (IL-10) were increased in Group TAP.


Resumo A resposta de defesa do hospedeiro ao desafio microbiano que emerge do sistema de canais radiculares leva à periodontite apical. Os objetivos deste estudo foram avaliar a expressão de citocinas pró e anti-inflamatórias e Óxido Nítrico (NO) por macrófagos após interação com Enterococcus faecalis no modo: planctônio e de biofilme desalojado; biofilme intacto estimulado por hidróxido de cálcio (CH), CH e clorexidina ou Pasta Tri Antibiótica (TAP). Para isto, a cultura de macrófagos originados de monócitos do sangue periférico de humanos (N=8) foi exposta aos diferentes tipos de bactéria por 24 horas. Então, a quantificação da produção de of Fator de Necrose Tumoral- alfa (TNF-α), interleucina (IL)-1β, IL-6, IL-10 e NO por macrófagos se deu por meio do Luminex xMAP e reação de Greiss, respectivamente. Além dos potenciais efeitos terapêuticos desses compostos, sua atividade antimicrobiana contra E. faecalis também foi testada através microscopia confocal. Os dados dos experimentos foram analisados através do teste de Kruskal-Wallis com Dunn`s post hoc (α < 0.05). Bactéria em modo de biofilme desalojado se mostrou mais agressivo ao sistema imune que as bactérias planctônicas e controle negativo induzindo a maior excreção de NO e TNF-α. Em relação ao biofilme intacto, a atividade antimicrobiana mais fraca ocorreu no grupo de CH. Os grupos CHX e TAP aumentaram significativamente a porcentagem de bactérias mortas na mesma extensão. Interessantemente, o biofilme por ele mesmo não induziu a liberação de citocinas pro-inflamatórias - exceto por NO - enquanto que o biofilme tratado com TAP ou pastas a base de CH aumentaram os níveis de IL-6; e TNF-α e IL-1 β respectivamente. Em contraste, os níveis da potente citocina anti-inflamatória (IL-10) foram aumentados pelo grupo TAP.


Subject(s)
Humans , Plankton , Biofilms , Root Canal Irrigants , Bacteria , Calcium Hydroxide , Chlorhexidine , Enterococcus faecalis , Anti-Bacterial Agents
18.
Article in English | LILACS-Express | LILACS | ID: biblio-1385761

ABSTRACT

ABSTRACT: Actually, bioceramic endodontic sealers represent an advantageous option for root canals sealing due to their multiple properties. There are several in vitro studies about their antimicrobial properties, however, their comparative antimicrobial potential, compared with other types of endodontic sealers is not entirely clear. The aim of the present research was to perform a systematic review to assess the antimicrobial effectiveness of commercially available bioceramic root canal sealers against Enterococcus faecalis in accordance with the PRISMA statement. A literature search was made in four electronic databases: MEDLINE, ClinicalKey, ScienceDirect and EBSCO. Were included in vitro studies, published in English, between 2015 and 2019. The evaluation of the selected studies was performed by two authors, independently. Four studies were included in the review. EndoSequence™ (Brasseler USA, Savannah, GA) also available on the market as TotalFill™ (Brasseler USA, Savannah, GA) and BioRoot™ were the bioceramic root canal sealers evaluated. The risk of bias was assessed according to the modified CONSORT checklist for preclinical studies. Selected studies reported positive antimicrobial activity against Enterococ cus faecalis in the bioceramic sealers evaluated. The less activity was observed on BioRoot™ sealer. All studies used a planktonic form of Enterococcus faecalis and in vitro laboratory methods of evaluation. The antimicrobial activity reported varies accordi ng to the laboratory method used, even for the same sealer, so the existence of false negatives is suggested. This systematic review identified the need for more research into the antimicrobial activity of bioceramic sealers, ideally with models using bacterial biofilms and studies in humans.


RESUMEN: En la actualidad, los cementos selladores biocerámicos son una opción ventajosa para la obturación de canales radiculares debido a sus múltiples propiedades. Existen varios estudios in vitro acerca de sus propiedades antimicrobianas, sin embargo, su potencial antimicrobiano, en comparación con otros tipos de cementos selladores endodónticos, no se está completamente claro. El propósito de la presente investigación fue realizar una revisión sistemática sobre las propiedades antimicrobianas de los cementos selladores biocerámicos disponibles en el mercado sobre Enterococcus faecalis, de acuerdo a la declaración PRIS- MA. Se realizó una búsqueda bibliográfica en cuatro bases de datos electrónicas: MEDLINE, ClinicalKey, ScienceDirect y EBSCO. Se incluyeron estudios in vitro, publicados en inglés, entre 2015 y 2019. La evaluación de los estudios seleccionados fue realizada por dos autores de forma independiente. Fueron incluidos cuatro estudios en la revisión. EndoSequence™ (Brasseler USA, Savannah, GA), también comercializado en el mercado como TotalFill™ (Brasseler USA, Savannah, GA), y BioRoot™ fueron los cementos selladores biocerámicos evaluados. El riesgo de sesgo se evaluó de acuerdo con la lista de verificación CONSORT modificada para estudios preclínicos. Los estudios seleccionados informaron actividadantimicrobiana positiva contra E. faecalis en los selladores biocerámicos evaluados. La menor actividad la presentó BioRootTM. Todos los estudios utilizaron una forma planctónica de E. faecalis y métodos de evaluación in vitro. La actividad antimicrobiana reportada varió en base al método de laboratorio utilizado, incluso para el mismo sellador, por lo que se sugiere la existencia de falsos negativos. Esta revisión sistemática identificó la necesidad de realizar más investigaciones sobre la actividad antimicrobiana de los selladores biocerámicos, idealmente con modelos que utilicen biopelículas bacterianas y estudios en humanos.

19.
Acta bioquím. clín. latinoam ; 55(2): 127-135, abr. 2021. tab
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1355555

ABSTRACT

Resumen Este trabajo tuvo como objetivo investigar la presencia de factores de virulencia, resistencia a antimicrobianos y metales pesados en 97 enterococos aislados de alimentos de origen animal. Se realizaron pruebas fenotípicas y genotípicas para la identificación a nivel de especie de los enterococos recuperados. Se identificaron 67 aislados como Enterococcus faecium, 9 como Enterococcus faecalis, 3 como Enterococcus durans, 2 como Enterococcus hirae y 1 como Enterococcus raffinosus. En 15 aislados no se logró su confirmación a nivel de especie por métodos fenotípicos ni genotípicos. La producción de exopolisacáridos fue el factor de virulencia más frecuente y se detectó en 46 enterococos, mientras que 19 exhibieron actividad gelatinasa y solo uno presentó beta-hemólisis. El estudio de la concentración inhibitoria mínima (CIM) reveló que 29 aislados resultaron resistentes a vancomicina (≥32 μg/mL) y 26 de ellos también eran resistentes a teicoplanina (≥32 μg/mL). Del total de aislamientos, 21 presentaron resistencia a tetraciclina (≥16 μg/mL) y sólo 3 exhibieron resistencia a la ampicilina (≥16 μg/mL). La prueba en agar reveló que 90 cepas resultaron resistentes al zinc (≥12 mM) mientras que todas exhibieron resistencia intermedia al cobre (4-12 mM). Los alimentos de origen animal actúan como reservorios de cepas de Enterococcus spp. que expresan factores de virulencia y resistencia a los antimicrobianos y a metales pesados. La implementación de una vigilancia continua en enterococos aislados de alimentos de origen animal es esencial para la salud humana.


Abstract This study was aimed at investigating the presence of virulence factors, antimicrobial and heavy metals resistance in 97 isolated enterococci from food of animal origin. Phenotypic and genotypic tests for species-level identification of the recovered isolates were carried out. This resulted in the iden tification of 67 strains as Enterococcus faecium, 9 as Enterococcus faecalis, 3 as Enterococcus durans, 2 as Enterococcus hirae and 1 as Enterococcus raffinosus. In 15 isolates, their confirmation at species level was neither achieved by phenotypic nor by genotypic methods. Exopolysaccharides production was the most frequent virulence factor and was detected in 46 isolates, while 19 displayed gelatinase activity, and only one resulted beta-hemolytic. Minimum inhibitory concentration (MIC) study revealed that 29 isolates were vancomycin-resistant (≥32 μg/mL), 26 of them were also resistant to teicoplanin (≥32 μg/mL). On the other hand, of the total isolates, 21 exhibited resistance to tetracycline (≥16 μg/mL) and only 3 enterococci exhibited resistance to ampicillin (≥16 μg/mL). Agar plates test revealed that 90 strains were resistant to zinc (≥12 mM) while all exhibited intermediate copper resistance (4-12 mM). Foods of animal origin act as a reservoir of Enterococcus spp. strains, expressing virulence factors, and resistance to antimicrobials and heavy metals. Therefore, the implementation of continuous surveillance in enterococci isolated from food of animal origin is essential to human health.


Resumo O objetivo deste trabalho foi investigar a presença de fatores de virulência, resistência a antimicrobianos e metais pesados em 97 enterococos isolados de alimentos de origem animal. Foram realizadas provas fenotípicas e genotípicas para a identificação no nível de espécie dos enterococos recuperados. Foram identificados 67 isolados como Enterococcus faecium, 9 de Enterococcus faecalis, 3 de Enterococcus durans, 2 como Enterococcus hirae e 1 como Enterococcus raffinosus. Em 15 isolados, sua confirmação em nível de espécie não foi alcançada por métodos fenotípicos e/ou genotípicos. A produção de exopolissacarídeos foi o fator de virulência mais frequente e se detectou em 46 enterococos, enquanto que 19 apresentaram atividade de gelatinase e somente um apresentou beta-hemólise. O estudo da concentração inibitória mínima (CIM) revelou que 29 isolados eram resistentes à vancomicina (≥32 μg/mL), e 26 deles foram também resistentes a teicoplanina (≥32 μg/mL). Pelo contrário, da quantidade total de isolamentos, somente 21 apresentaram resistência à tetraciclina (≥16 μg/mL) e apenas 3 cepas apresentaram resistência à ampicilina (≥16 μg/mL). A prova em ágar revelou que 90 cepas eram resistentes ao zinco (≥12 mM), enquanto que todas mostraram resistência intermediária ao cobre (4-12 mM). Os alimentos de origem animal atuam como reservatórios de cepas de Enterococcus spp. os quais expressam fatores de virulência, e resistência aos antimicrobianos e a metais pesados. A implementação de uma vigilância contínua em enterococos isolados de alimentos de origem animal é essencial para a saúde dos humanos.

20.
Salud UNINORTE ; 37(1): 84-95, ene.-abr. 2021. tab, graf
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1365969

ABSTRACT

RESUMEN Introducción: El objetivo final de la terapia endodóntica es la eliminación de microorganismos en el interior del conducto radicular. Algunas bacterias pueden sobrevivir a esta terapia; entre las comúnmente detectadas en fallas endodónticas se encuentra el E. faecalis. Los cementos biocerámicos se presentan como una alternativa eiciente en la terapia de endodoncia debido a su capacidad de sellado y sus propiedades antimicrobianas. Métodos: Estudio experimental in vitro, en el que se sembró la bacteria (E. faecalis) en una caja de Petri, se tomaron de 5 a 10 cepas y se disolvieron en caldo de Mueller Hinton, para luego depositar el inóculo más el cemento en diferentes concentraciones en una placa de 96 pozos y medir el nivel de absorbancia de cada muestra en un espectrofotómetro. Para el análisis estadístico se utilizaron las pruebas no paramétricas de Mann-Whitney y Kruskal Wallis. Resultados: Al comparar de dos en dos los datos obtenidos no se obtuvieron datos estadísticamente signiicativos, pero al comparar las diferentes concentraciones de ambos cementos se obtuvo que el cemento de Grossman al 2 % tiene mayor efecto antimicrobiano. Conclusión: No hay diferencia significativa en la actividad antimicrobiana del MTA Fillapex® y el cemento de Grossman contra el E. faecalis, pero al comparar el cemento de Grossman al 2 %, este tiene mayor efecto antimicrobiano en comparación con el mismo cemento a diversas concentraciones.


ABSTRACT Introduction: the ultimate aim of the root canal therapy is elimination or reduction of microorganisms in the root canal system. Although some bacteria can survive this therapy, among bacteria commonly detected in endodontic failure is E. faecalis. The bioceramic sealers are presented as an efficient alternative due its sealing capacity and antimicrobial properties. Methods: the research was experimental in vitro, where the bacteria (E. faecalis) was planted in a box perti, were taken from 5 to 10 strains and dissolved in broth Mueller Hinton then deposit inoculum more sealer at different concentrations in a 96 well plate and measuring the level of absorbance of each sample in a spectrophotometer. For statistical analysis, non-parametric Mann-Whitney and Kruskal Wallis were used. Results: when comparing two at the data no statistically significant data were obtained, but when comparing different concentrations of both sealers was obtained Grossman sealers 2% has greater antimicrobial effect. Conclusion: There is not significant difference in the antimicrobial activity of MTA Fillapex and Grossman cement against E. faecalis, but when comparing Grossman cement to 2%, this has a higher antimicrobial effect compared to the same cement at various concentrations.

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