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This research work investigated modulatory effects of Bryophylum pinnatum extract on BDNF expression, and cognitive functions in repetitive pain-induced oxidative stress in Wistar rats. Animals weighing between 80–100g were acquired from the animal house of the Department of Human Physiology, Faculty of Basic Medical Sciences, University of Port Harcourt, and all animals received standard laboratory rat feeds and water ad libitum. The study was designed to assess the time dependent effects with a total of 30 rats divided into 6 groups. Group 1(Control), Group 2 (Pain Only), Group 3 (Pain + 5mg/kg Morphine), Group 4 (Pain + 10mg/kg Morphine), Group (Pain + 25mg/kg Bryophylumm Pinnatum), Group 6 (Pain + 50mg/kg Bryophylumm Pinnatum), Hydromethanolic extracts was prepared accordingly, and Gas Chromatography Mass Spectrometry (GC/MS) analysis were carried out. Neurobehavioral studies were conducted weekly to assess the effects of the interventions on cognitive and neurological parameters, using radial maze and navigational maze test. Assay of BDNF was done using the Elisa method. The Results showed that Morphine and Bryophyllum pinnatum both significantly improved BDNF expression, showed antioxidant effects, improved cognitive functions, and provided possible mechanisms of pain relief. Pharmacokinetic studies on the binding affinities and drug-likeness properties of the active compounds from these extracts revealed some favorable properties with regard to management of oxidative stress and promotion of cognitive function in states of pain. The study therefore provide indication of the therapeutic potential of Bryophyllum pinnatum in the effective management of Pain, oxidative stress and cognitive functions.

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Background: Lung cancer is the most common cause of cancer mortality worldwide. Smoking is undoubtedly the major risk factor of lung cancer in both genders. Adenocarcinoma is the most common form of lung cancer in both men and women and the most prevalent subtype in non-smokers. Lung cancer in never-smokers is a distinct entity with sparse studies. We studied the clinico-pathologic profile of lung adenocarcinoma and pattern of p53 expression in smokers and non-smokers. Methods: A prospective study involving 100 lung adenocarcinoma cases from January 2020 to June 2021 examined p53 expression using immunohistochemistry. Trucut biopsies, fine needle aspiration cytology (FNAC) cell blocks, and pleural effusion were analyzed to identify the predominant morphological subtype of the lung adenocarcinoma. Results: The most common histological pattern of lung adenocarcinoma was solid, and the presenting symptoms were cough and dyspnoea in both smokers and non-smokers. The incidence of lung adenocarcinoma was higher in non-smokers in the study. p53 expression had a significant correlation with smoking but not with stage of disease or morphological subtype of lung adenocarcinoma. Conclusions: p53 mutation has a statistical correlation with smoking in adenocarcinomas in our population. Among the adenocarcinoma cases in our study, non-smokers predominate (n=53). Even though our study showed the p53 mutation has no statistical correlation with the stage of the disease or histological subtype in adenocarcinoma, more cases need to be studied to prove this observation.

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Single-cell RNA sequencing (scRNA-Seq) technology provides the scope to gain insight into the interplay between intrinsic cellular processes as well as transcriptional and behavioral changes in gene–gene interactions across varying conditions. The high level of scarcity of scRNA-seq data, however, poses a significant challenge for analysis. We propose a complete differential co-expression (DCE) analysis framework for scRNA-Seq data to extract network modules and identify hub-genes. The performance of our method has been shown to be satisfactory after validation using an scRNA-Seq esophageal squamous cell carcinoma (ESCC) dataset. From comparison with four other existing hub-gene finding methods, it has been observed that our method performs better in the majority of cases and has the ability to identify unique potential biomarkers that were not detected by the other methods. The potential biomarker genes identified by our framework, differential co-expression analysis method for single-cell RNA sequencing data (scDiffCoAM), have been validated both statistically and biologically.

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La necesidad de buscar alternativas que permitan la inclusión de los estudiantes con diferentes discapacidades en la práctica de la Educación Física induce a que se utilice la danza contemporánea como expresión corporal, pues impacta en el desarrollo y mejora la calidad de vida, en lo físico y psicológico, al expresarse emociones y sentimientos a través del movimiento. El trabajo que se presenta tiene como finalidad diseñar una estrategia de inclusión para estudiantes con discapacidades múltiples donde la Educación Física utilice la danza contemporánea como expresión corporal en las aulas especializadas, en la unidad educativa "Dolores Cacuango" de la parroquia Pascuales, Guayas-Guayaquil. Para el estudio se aplicaron métodos científicos como la revisión documental, la entrevista, la encuesta y la observación para recopilar información acerca de los estudiantes con diferentes discapacidades, y de los docentes involucrados en las clases de Educación Física y su combinación con la danza contemporánea. Como resultados, se diseña una estrategia de inclusión para estudiantes con discapacidades múltiples donde la Educación Física utiliza la danza contemporánea como expresión corporal. Se concluyó que las dinámicas de las actividades danzarías propuestas en la estrategia, se fundamentaron sobre bases científicas, y en correspondencia con la relación entre actividad física, condición física y salud, para fortalecer los músculos, mejorar el equilibrio, la coordinación del movimiento, la inclusión a la sociedad, y el compartir un mismo proyecto humano.

A necessidade de buscar alternativas que permitam a inclusão de alunos com diferentes deficiências na prática da Educação Física leva à utilização da dança contemporânea como expressão corporal, uma vez que impacta o desenvolvimento e melhora a qualidade de vida, física e psicológica, quando as emoções e os sentimentos são expressos através do movimento. O objetivo do trabalho apresentado é desenhar uma estratégia de inclusão de alunos com deficiência múltipla onde a Educação Física utiliza a dança contemporânea como expressão corporal em salas de aula especializadas, na unidade educacional "Dolores Cacuango" da freguesia de Pascuales, Guayas-Guayaquil. Para o estudo foram aplicados métodos científicos como revisão documental, entrevista, levantamento e observação para coletar informações sobre alunos com diferentes deficiências e professores envolvidos nas aulas de Educação Física e sua combinação com a dança contemporânea. Como resultado, desenha-se uma estratégia de inclusão para alunos com deficiência múltipla onde a Educação Física utiliza a dança contemporânea como expressão corporal. Concluiu-se que a dinâmica das atividades de dança propostas na estratégia foram baseadas em bases científicas, e em correspondência com a relação entre atividade física, condição física e saúde, para fortalecer os músculos, melhorar o equilíbrio, a coordenação do movimento, a inclusão na sociedade, e compartilhando o mesmo projeto humano.

The need to look for alternatives that allow the inclusion of students with different disabilities in the practice of Physical Education leads to the use of contemporary dance as body expression, since it impacts development and improves the quality of life, physically and psychologically, when emotions and feelings are expressed through movement. The purpose of the work presented is to design an inclusion strategy for students with multiple disabilities where Physical Education uses contemporary dance as body expression in specialized classrooms, in the "Dolores Cacuango " educational unit of the Pascuales parish, Guayas-Guayaquil. For the study, scientific methods such as documentary review, interviews, surveys and observations were applied to collect information about students with different disabilities, and teachers involved in Physical Education classes and their combination with contemporary dance. As results, an inclusion strategy is designed for students with multiple disabilities where Physical Education uses contemporary dance as body expression. It was concluded that the dynamics of the dance activities proposed in the strategy were based on scientific bases, and in correspondence with the relationship between physical activity, physical condition and health, to strengthen muscles, improve balance, coordination of movement, inclusion in society, and sharing the same human project.

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SUMMARY: As the economy develops and living standards improve, overweight and obesity are increasingly prevalent. Currently, weight-loss medications are primarily administered orally or intravenously, which can result in poor targeting, low bioavailability, frequent administration, and high toxicity and side effects. The study aimed to address these challenges by preparing polylactic acid- polyethylene glycol staple fibers that carry the browning drug pioglitazone hydrochloride using electrostatic spinning and freeze-cutting techniques. Animal experiments were conducted to test the effectiveness of these fibers. Additionally, the study investigated the expression of uncoupling protein genes in rats exposed to different water temperatures by measuring changes in serum urea nitrogen and mRNA expression levels of skeletal muscle uncoupling protein genes. The physiological and genetic effects of low-temperature swimming exercise on changes in energy metabolism in rats were also analyzed at both the individual and molecular levels. The results revealed that serum urea nitrogen remained more stable in hypothermic swimming rats compared to rats in the swimming group. Furthermore, the study observed an induced up-regulation of uncoupling proteins in the skeletal muscle of Wistar rats in response to external temperature stimulation, and the expression of mRNA for skeletal muscle uncoupling proteins significantly increased as the temperature decreased. And the prepared short nanofibers also had a significant promotive effect on uncoupling protein gene, COX7A1, while suppressing the expression of lipogenic gene.

A medida que la economía se desarrolla y los niveles de vida mejoran, el sobrepeso y la obesidad son cada vez más frecuentes. Actualmente, los medicamentos para bajar de peso se administran principalmente por vía oral o intravenosa, lo que puede resultar en una mala focalización, baja biodisponibilidad, administración frecuente y alta toxicidad y efectos secundarios. El estudio tuvo como objetivo abordar estos desafíos mediante la preparación de fibras cortadas de ácido poliláctico y polietilenglicol que transportan el fármaco pardo clorhidrato de pioglitazona mediante técnicas de hilado electrostático y liofilización. Se realizaron experimentos con animales para probar la eficacia de estas fibras. Además, el estudio investigó la expresión de genes de proteínas desacopladoras en ratas expuestas a diferentes temperaturas del agua midiendo los cambios en el nitrógeno ureico sérico y los niveles de expresión de ARNm de genes de proteínas desacopladoras del músculo esquelético. También se analizaron los efectos fisiológicos y genéticos del ejercicio de natación a baja temperatura sobre los cambios en el metabolismo energético en ratas, tanto a nivel individual como molecular. Los resultados revelaron que el nitrógeno ureico sérico permaneció más estable en ratas nadadoras hipotérmicas en comparación con las ratas del grupo de natación. Además, el estudio observó una regulación positiva inducida de las proteínas desacopladoras en el músculo esquelético de ratas Wistar en respuesta a la estimulación de la temperatura externa, y la expresión de ARNm para las proteínas desacopladoras del músculo esquelético aumentó significativamente a medida que disminuía la temperatura. Además, las nanofibras cortas preparadas también tuvieron un efecto promotor significativo sobre el gen de la proteína de desacoplamiento, COX7A1, al tiempo que suprimieron la expresión del gen lipogénico.

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Aim: Neuroendocrine tumors are heterogenous group of neoplasms that includes benign and malignant tumors that originate from neuroendocrine or nonneuroendocrine organs. Insulinoma?associated protein 1 (INSM1) is a zinc finger transcription factor originally isolated from subtraction library of human insulinoma. The main aim was to study the INSM1 expression in a spectrum of neuroendocrine tumors and a limited spectrum of nonneuroendocrine tumors. Materials and Methods: A total of 100 cases of which 57 neuroendocrine neoplasms and 43 nonneuroendocrine neoplasms were included in the study. Immunohistochemistry (IHC) was done and expression patterns of INSM1 were analyzed. Pituitary adenoma, medullary carcinoma of thyroid, pheochromocytoma lung, gastrointestinal, and pancreatic neuroendocrine tumors were the neuroendocrine tumors that were included in the study. Papillary carcinoma of thyroid, gastrointestinal adenocarcinoma, lung adenocarcinoma, and squamous cell carcinoma were the nonneuroendocrine tumors that were included in the study. Depending upon the tissue availability, comparison of INSM1 with synaptophysin and chromogranin was also done in few neuroendocrine tumors. Results: All the 57 neuroendocrine tumors showed positive expression for INSM1 and all the nonneuroendocrine tumors were negative for INSM1. This study is statistically significant. Conclusion: Our study suggests that INSM1 is a diagnostic marker for neuroendocrine tumors with high degree of sensitivity and specificity. The study is significant and suggests that INSM1– IHC shows nuclear positivity in a spectrum of neuroendocrine tumors. Being a nuclear marker, interpretation is easy and more reliable than the cytoplasmic markers. INSM1 has a stronger positivity than synaptophysin and chromogranin in the present study especially for small cell carcinoma lung. Hence, INSM1 may be included in the routine panel for neuroendocrine tumors along with synaptophysin and chromogranin.

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Background: Angiogenesis plays a role in both physiological and pathological processes such as wound healing, embryonic development, and cancer metastasis, and it is the process by which new blood vessels are formed from existing ones. Diosgenin is a naturally occurring steroidal sapogenin found in plants such as yam and fenugreek, which is a compound of interest because of its pharmacological properties, including anti-carcinogenic effects. Zebrafish embryos are used due to their translucent nature, which facilitates the direct observation of vascular development. Aims and Objectives: The purpose of this study is to utilize zebrafish embryos to examine the impact of diosgenin on angiogenesis. It specifically aims to determine whether diosgenin could inhibit the development of inter-segmental vessels (ISV) in zebrafish embryos and also to investigate its effect on vascular endothelial growth factor (VEGF-A), a key regulator of angiogenesis. Materials and Methods: To explore the anti-angiogenic properties of diosgenin, we performed in vivo experiments using the zebrafish angiogenesis model. This involved staining red blood cells with O-dianisidine and conducting semi- quantitative real-time polymerase chain reaction analysis to assess VEGF-A mRNA expression levels. Results: Diosgenin was shown to hinder the growth and formation of ISV in dosage-dependent development using a red blood cell staining assay when compared to the positive control, SU5416. In addition, there was a significant decrease in VEGF-A expression. Conclusion: In the presence of diosgenin, there is an evidence of inhibition of ISV development and suppression of VEGF mRNA expression. These findings underscore the therapeutic potential of diosgenin for targeting angiogenesis in cancer. However, further research is needed to understand the mechanism underlying diosgenin’s effect and its clinical relevance.

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RESUMEN Introducción. Las células dendríticas (CD) desempeñan un papel clave en la presentación antigénica y la activación de linfocitos T, pero su función puede ser modulada por el microambiente tumoral, lo que afecta la respuesta inmunitaria antitumoral. Este estudio se centra en la interacción entre las CD y el hepatocarcinoma (HCC), explorando cómo el entorno tumoral influye en la actividad de las CD. Objetivo. Evaluar la variación en la actividad de las CD en respuesta a la expresión de citoquinas proinflamatorias, IL-10 y receptores CXCR4 y CCR7 en un modelo murino de hepatocarcinoma (PM299L). Métodos. Se realizaron ensayos in vitro cocultivando CD murinas y una línea tumoral murina de HCC. Se evaluó la expresión de citoquinas proinflamatorias (IL-12, IL-6, IL-1β, TNF-α), inmunosupresora (IL-10) y receptores asociados a migración y maduración (CXCR4 y CCR7) mediante Qpcr a las 24, 48 y 72 horas. Los ensayos se repitieron tres veces. Resultados. Las CD expuestas al entorno tumoral de HCC mostraron una mayor expresión de citoquinas proinflamatorias y IL-10 en comparación al grupo control. Además, se observó una expresión elevada de receptores CXCR4 y CCR7 en las CD expuestas al HCC. Estos cambios en la expresión de genes ocurrieron en un período de 72 horas de cocultivo. Conclusión. La actividad de las CD se ve influenciada por el entorno tumoral de HCC y el tiempo de interacción, lo que modula su función proinflamatoria y de presentación antigénica. Estos hallazgos destacan la importancia de comprender la dinámica de la respuesta inmunitaria en el hepatocarcinoma.

ABSTRACT Introduction. Dendritic cells (DCs) play a key role in antigen presentation and T cell activation, but their function can be modulated by the tumor microenvironment, affecting the antitumor immune response. This study focuses on the interaction between DCs and hepatocellular carcinoma (HCC), exploring how the tumor environment influences DC activity. Objective. To evaluate the variation in DC activity in response to the expression of proinflammatory cytokines, IL-10 and CXCR4 and CCR7 receptors in a murine model of hepatocellular carcinoma (PM299L). Methods. In vitro assays were performed co-culturing murine DCs and HCC tumor line. The expression of proinflammatory cytokines (IL-12, IL-6, IL-1β, TNF-α), immunosuppressive (IL-10) and receptors associated with migration and maturation (CXCR4 and CCR7) was evaluated by Qpcr at 24, 48 and 72 hours. The tests were repeated three times. Results. DCs exposed to the HCC tumor environment showed increased expression of proinflammatory cytokines and IL-10 compared to the control group. Furthermore, elevated expression of CXCR4 and CCR7 receptors will be observed in DCs exposed to HCC. These changes in gene expression occurred within a 72-h period of coculture. Conclusion. DC activity is influenced by HCC tumor environment and interaction time, which modulates their proinflammatory and antigen presentation function. These findings highlight the importance of understanding the dynamics of the immune response in hepatocarcinoma.

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Gastric cancer (GC) is one of the most common malignant tumors with high incidence and mortality rates. Most patients with GC are not diagnosed until the advanced stage of cancer or during tumor screening, resulting in missing the best treatment time. This study identified key modules and hub genes associated with GC by weighted gene co-expression network analysis (WGCNA). The "limma" package in R was used to identify differentially expressed genes (DEGs) in GC samples from TCGA, and a total of 4892 DEGs were identified. GO enrichment and KEGG pathway enrichment analyses were conducted to detect the related pathways and functions of DEGs. These DEGs were primarily associated with extracellular matrix organization, DNA replication, cell cycle, and p53 signaling pathway. Gene modules associated with clinical characteristics were identified with WGCNA in tumor and normal samples. Six gene modules were obtained in the WGCNA network, of which two modules were significantly correlated with GC. Hub genes of key modules were identified using survival analysis and expression analysis. Finally, one-way ANOVA was used to explore the relationship between hub gene expression in normal tissues and different pathological stages of GC. Through survival and expression analysis, a total of 19 genes with good prognosis and significantly differential expressed were identified. The hub genes were significantly differential expressed in normal tissues and different pathological stages of GC, indicating that these genes have important diagnostic value for early GC and can be used as auxiliary indicators in the diagnosis of early GC.

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Cell type-specific expression of genes plays a pivotal role in the development and evolution of multicellular organisms over millions of years. The majority of regulatory control resides within the non-coding regions of the genome, referred to as ‘dark matter’, which contains cis-regulatory modules. These cis-regulatory modules function collectively and can impact gene expression even when located far from the target gene, exhibiting context-specific behaviour. Consequently, the cis-regulatory code governing gene expression patterns is intricate, in contrast to the universally understood genetic code. This overview centres on the current knowledge regarding cis-regulatory elements, primarily enhancers and their role in governing the spatiotemporal gene expression patterns, and how they have evolved and adapted across different species.

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Introduction: Ovarian carcinomas is still maintaining pivotal role among gynecological malignancy regarding cancer death in women. This study aims to observe expression pattern of VEGF, Her2/Neu and Ki-67 in lieu of targeted therapy for ovarian carcinoma. Materials and Methods: It was retrospective observational study carried out for last 3 years (2020-2022). A total of 160 cases of Ovarian tumors were received from different departments. The specimens were processed, histopathological sections were examined under Hematoxylin and Eosin stain, Immunohistochemical stains like VEGF, Her2/Neu and Ki-67. Evaluation was done by trained and independent pathologists. Correlation of Histological type, grade, age group. FIGO stage with expression of VEGF, Her2/Neu, and Ki-67 done. Results: Out of 160 specimens of histological examination proved 143 EOC- serous type constituted maximum (53.1 %) number. Among them (63.7%) were high grade and most of the cases belonged to 41.3% (59/143) in FIGO stage I in our study. Grade 3+ HER2/neu immunostaining was identified in 22.37% cases and had significantly correlated with tumor grade (?2 = 19.7981 with Yates correction; P <0.00001) and FIGO Staging (p=0.00024). Among High grade EOC, High proliferation index (HPI) was 19.5% for Ki-67. we could observe significant statistical association of Ki-67 HPI and tumor differentiation. Moreover, significant correlations was found between the high-grade EOC and HPI of Ki-67/Her2- neu co-expression (p<0.05). Though significant association was found between tumor grade and VEGF expression (?2 = 11.1041; P = .000861) but no correlation were in VEGF/Her-2/neu HPI and the degree of tumor differentiation (chi-square, p>0.05). Conclusion: Role of Her2/Neu and Ki-67 expression and their association should be considered in the progression and tumor grade and stage of EOC.

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Phosphodiesterase 2A (PDE2A) plays a pivotal role in modulating cyclic nucleotide metabolism. Recent studies have shown that PDE2A is associated with some tumors, but its expression profiles, prognostic significance, and immunological roles in diverse cancer types remain unclear. Utilizing advanced bioinformatics tools, we performed a comprehensive analysis of PDE2A gene expression in multiple human cancers. Our study revealed that PDE2A expression was significantly reduced in the majority of cancer types and clinicopathological stages (I to IV) compared to normal tissues. Additionally, PDE2A expression was closely related to the prognosis of cancers such as stomach adenocarcinoma (STAD), ovarian serous cystadenocarcinoma (OV), and liver hepatocellular carcinoma (LIHC). Cox regression analyses indicated that PDE2A can act as an independent prognostic factor for these cancers. The level of PDE2A DNA methylation was significantly decreased in most cancers. Genetic alterations in PDE2A predominantly manifest in the form of amplifications. Moreover, infiltrating cells and immune checkpoint genes, including PDCD1, exhibited notable correlations with PDE2A expression. Significant associations were observed between PDE2A expression and tumor mutation burden as well as microsatellite instability. Single cell sequencing revealed PDE2A's crucial role in regulating differentiation and angiogenesis of cancer cells. Functional enrichment analysis emphasized the important role of PDE2A in synaptic transmission and tumor development. Aberrant expression of PDE2A influenced the sensitivity of various antitumor and chemotherapy drugs. This research provided a comprehensive analysis of PDE2A in human cancers, highlighting its potential as both a prognostic marker and an immunotherapy target for future research.

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Background: Evaluation of the diagnostic performance of estimated expression levels of microRNAs 203a, 206 and 576 in the cellular aspirate of solitary thyroid nodule (STN) in comparison to cytological examination for identification of malignant TN. Methods: The 74 patients with STN underwent clinical and US evaluation and gave blood samples for estimation of serum levels of thyroglobulin (TG) and anti-thyroglobulin antibodies (ATG). Then, fine-needle aspiration was performed to obtain cellular aspirate for cytological examination and estimation of levels of microRNAs. The appropriate surgical procedure was undertaken and the excised specimens were sent for pathological diagnosis. Results: Pathologically, 19 nodules had papillary thyroid carcinoma (PTC), 6 nodules had follicular carcinoma and one medullary carcinoma, while 48 nodules were benign nodules. Cytological examination diagnosed malignancy in 5 specimens, while specimens were suspicious of malignancy, 25 specimens were diagnosed as benign and 14 specimens as non-diagnostic. Serum levels of TG and ATG were significantly higher with malignancy. Expression levels of miR-203a and 206 were significantly lower, while miR-576 were significantly higher in malignant than benign aspirates. Multivariate regression analysis showed more significant ability for miR-576 level than cytological examination as screening and for miR-203a than miR-206 as diagnostic for malignant aspirate and for PTC Conclusions: Estimation of expression levels of microRNAs in cellular aspirate of STN is feasible and accurate for detection of the malignancy. Down-expression of miR-203a and over-expression of miR-576 in tissue aspirate are more diagnostic for TC and can specify PTC cases out of other TC cases.

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The current eccentric environment changes are causing serious impacts on food crops, including rice. Such environmental circumstances encompass abiotic stress conditions such as drought, cold, heat, and inappropriate levels of metals. To cope with abiotic stresses, plants initiate various metabolic pathways that modify themselves according to such stresses. In this study, we have computationally analyzed genes in rice that exhibit differential expression under various abiotic stress conditions. A widely used database was accessed for gene expression data on rice in response to drought, salt, and cold stresses. The research uncovered that 7722 genes, 3040 genes, and 1705 genes were expressed differently in rice under drought, salt, and cold conditions, respectively. Two hundred and twenty-two up-regulated and 58 down-regulated genes were identified which express under all stresses and were regarded as significant differentially expressed genes. Functional annotation showed that these genes encode beta-amylase, cytochrome P450, cyclin-dependent protein kinases, and the NAC domain-containing protein which plays crucial roles during stress. Pathway enrichment analysis revealed that significant genes participate in biological pathways such as the phenylpropanoid pathway, abscisic acid signaling, cell wall organization, and choline biosynthesis. Thus, our

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Background and Objectives: Despite recent advances in understanding the gastric cancer (GC) biology, the precise molecular mechanism of gastric carcinogenesis and role of deregulated immune responses in GC progression are still not well understood. In this study, mRNA levels of human leukocyte antigen (HLA)?DRA and ?DQA1 were assessed in GC patients to find a potential association between expression of these HLA?II molecules and gastric carcinogenesis. Methods: Using quantitative real?time (qRT)?PCR, mRNA levels of HLA?DRA and ?DQA1 were assessed in 20 pairs of matched GC and normal tissues. Results: Our results showed that overall mRNA level of HLA?DRA was decreased in the tumor samples relative to control tissues (median fold change [FC] = 0.693; P = 0.445). Overall HLA?DQA1 level was increased in the tumor samples relative to control tissues (median FC = 1.659; P = 0.5117). However, the mentioned data were not statistically significant. Meanwhile, using a ? 2.5 FC as the cutoff to determine upregulation or downregulation, 35% of patients showed a downregulated expression of HLA?DRA, while 10% of those showed upregulation in HLA?DRA expression. Upregulation and downregulation of HLA?DQA1 expression were detected, respectively, in 35% and 25% of samples. A strong positive correlation was determined between HLA?DRA and HLA?DQA1 levels in tumor tissues ( r = 0.7298; P = 0.0003). Conclusion: The results reported here along with future studies can be useful to understand the interplay between immune system and GC, therefore, may be helpful to design an effective immune?based therapy.

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Objective To screen key autophagy-related genes in alcoholic hepatitis (AH) and investigate potential biomarkers and therapeutic targets for AH. Methods Two AH gene chips in Gene Expression Omnibus (GEO) and autophagy-related data sets obtained from MSigDB and GeneCards databases were used, and the key genes were verified and obtained by weighted gene co-expression network analysis (WGCNA). The screened key genes were subject to gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), protein-protein interaction (PPI) and immune infiltration analyses. Messenger RNA (mRNA)- microRNA (miRNA) network was constructed to analyze the expression differences of key autophagy-related genes during different stages of AH, which were further validated by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) in the liver tissues of AH patients and mice. Results Eleven autophagy-related genes were screened in AH (EEF1A2, CFTR, SOX4, TREM2, CTHRC1, HSPB8, TUBB3, PRKAA2, RNASE1, MTCL1 and HGF), all of which were up-regulated. In the liver tissues of AH patients and mice, the relative expression levels of SOX4, TREM2, HSPB8 and PRKAA2 in the AH group were higher than those in the control group. Conclusions SOX4, TREM2, HSPB8 and PRKAA2 may be potential biomarkers and therapeutic targets for AH.

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Cellulose synthase (CesA), one of the key enzymes in the biosynthesis of cellulose in plants, plays an important role in plant growth and plant resistance. In this study, a total of 21 AsCesA genes from Aquilaria sinensis were systematically identified and the physico-chemical characteristics were analyzed based on genome database and bioinformatical methods. The phylogenetic tree was constructed and the gene location on chromosome, cis-acting elements in the 2 000 basepairs upstream regulatory regions and conservative motifs were analyzed. The AsCesA proteins were mainly located on the plasma membrane. The number of amino acids of the proteins ranged from 390 to 1 261. The isoelectric point distributed from 5.67 to 8.86. All of the 21 AsCesA proteins possessed the transmembrane domains, the number of which was from 6 to 8. The genes were classified into 3 groups according to the phylogenetic relationship. Obvious differences were observed in motif composition in genes from different groups. However, motif2, motif6, motif7 and motif10 were observed in all of AsCesA proteins. Analysis of cis-acting elements indicated that AsCesA genes family has cis-acting elements related to plant hormones, abiotic stresses, and biological processes. Seven AsCesA genes with differential expression were selected according to the calli transcriptome data induced by NaCl at different times and their expression levels under different abiotic stresses were analyzed by quantitative real-time PCR. The results indicated that salt, low temperature, drought, and heavy metal stresses could affect the expression level of AsCesA genes, and the abundance of AsCesA1, AsCesA3 and AsCesA20 showed a significant change, implying their potential important roles to the abiotic stresses. The accumulation pattern of cellulose content under different abiotic stresses was similar to the expression trend of AsCesA genes. Our results provide valuable insights into the role of cellulose synthase in A.sinensis in plant defense.

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@#Lung adenocarcinoma is a prevalent histological subtype of non-small cell lung cancer with different morphologic and molecular features that are critical for prognosis and treatment planning. In recent years, with the development of artificial intelligence technology, its application in the study of pathological subtypes and gene expression of lung adenocarcinoma has gained widespread attention. This paper reviews the research progress of machine learning and deep learning in pathological subtypes classification and gene expression analysis of lung adenocarcinoma, and some problems and challenges at the present stage are summarized and the future directions of artificial intelligence in lung adenocarcinoma research are foreseen.

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OBJECTIVE@#To obtain detailed understanding on the gene regulation of natural compounds in altering prognosis of head and neck squamous cell carcinomas (HNSC).@*METHODS@#Gene expression data of HNSC samples and peripheral blood mononuclear cells (PBMCs) of HNSC patients were collected from Gene Expression Omnibus (GEO). Differential gene expression analysis of GEO datasets were achieved by the GEO2R tool. Common differentially expressed gerres (DEGs) were screened by comparing DEGs of HNSC with those of PBMCs. The combination was further analyzed for regulating pathways and biological processes that were affected.@*RESULTS@#Totally 110 DEGs were retrieved and identified to be involved in biological processes related to tumor regulation. Then 102 natural compounds were screened for a combination such that the expression of all 110 commonly DEGs was altered. A combination of salidroside, ginsenoside Rd, oridonin, britanin, and scutellarein was chosen. A multifaceted, multi-dimensional tumor regression was showed by altering autophagy, apoptosis, inhibiting cell proliferation, angiogenesis, metastasis and inflammatory cytokines production.@*CONCLUSIONS@#This study has helped develop a unique combination of natural compounds that will markedly reduce the propensity of development of drug resistance in tumors and immune evasion by tumors. The result is crucial to developing a combinatorial natural therapeutic cocktail with accentuated immunotherapeutic potential.

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WRKYs is a unique family of transcription factors (TFs) in plants, and belongs to the typical multifunctional regulator. It is involved in the regulation of multiple signaling pathways. This type of transcription factor is characterized to contain about 60 highly conservative amino acids as the WRKY domain, and usually also has the Cys2His2 or Cys2His-Cys zinc finger structure. WRKYs can directly bind to the W-box sequence ((T)(T) TGAC (C/T)) in the promoter region of the downstream target gene, and activate or inhibit the transcription of the target genes by interacting with the target protein. They may up-regulate the expression of stress-related genes through integrating signal pathways mediated by abscisic acid (ABA) and reactive oxygen species (ROS), thus playing a vital role in regulating plant response to abiotic stresses. This review summarizes the advances in research on the structure and classification, regulatory approach of WRKYs, and the molecular mechanisms of WRKYs involved in response to drought and salt stresses, and prospects future research directions, with the aim to provide a theoretical support for the genetic improvement of crop in response to abiotic stresses.