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1.
Article in Chinese | WPRIM | ID: wpr-920377

ABSTRACT

Objective In order to understand the contamination status of food-borne pathogens in food in Nantong City, and to provide a scientific basis for food safety risk assessment and supervision. Methods The monitoring was performed according to the plan of food safety risk surveillance in Nantong City and the manual on monitoring microbes and its pathogenic factors in foodstuffs of Jiangsu Province. A total of 1025 food samples of 11 kinds were monitored from 2017 to 2020 for detecting of pathogenic bacteria such as Listeria monocytogenes, Salmonella, Vibrio parahaemolyticus, Bacillus cereus and Staphylococcus aureus, and the test results were summarized, analyzed and evaluated. Results A total of 142 pathogenic bacteria were detected, with a total positive rate of 13.85%. The detection rate was the highest in 2018 (25.65%), and the lowest in 2017 (4.98%). The highest detection rate was found in meat and meat products(25.38%), followed by aquatic products and animal products (17.92%), cooked rice and flour products around the school (14.29%), the detection rate of barreled drinking water was the lowest (2.86%). 170 strains of pathogenic bacteria were detected from 1025 samples. The higher positive rate was Bacillus cereus in the cooked rice flour products around the school, and 5 strains of Bacillus cereus were detected, with the detection rate was 14.29%. 18 strains of Staphylococcus aureus were detected in meat and meat products, and the detection rate was 13.85%; 70 Vibrio parahaemolyticus were detected in aquatic products, and the detection rate was 13.49%; 16 Listeria monocytogenes were detected in meat and meat products, and the detection rate was 12.31%. The positive rate of catering links is higher than that of circulation links, and the positive rate of urban areas is higher than that of rural areas. Conclusions Pathogenic bacteria were detected in different kinds of food, and the overall situation of food safety is stable. In the future, the monitoring scope will be expanded continuously, the monitoring continuity will be strengthened, the sampling quantity will be increased, the monitoring quality will be improved, the trend of food pollution change in the city shall be mastered in time, food safety warning shall be well done, and food safety education shall be strengthened for food practitioners, food pollution risk shall be reduced and food safety shall be effectively guaranteed.

2.
rev. udca actual. divulg. cient ; 24(2): e2139, jul.-dic. 2021. tab
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1361220

ABSTRACT

RESUMEN El fríjol caupí es una leguminosa importante en la región Caribe de Colombia, por su contribución a la dieta de sus pobladores y por ser una alternativa de ingresos para pequeños productores; sin embargo, los rendimientos obtenidos no superan los 600 kg ha-1, debido al desconocimiento del efecto de las prácticas culturales en la especie, como en los diversos cultivares. El objetivo del estudio fue evaluar el efecto de diferentes espaciamientos e hidrogel sobre características vegetativas y reproductivas del fríjol caupí cultivar Caupicor 50, en el departamento de Sucre, del Caribe colombiano. Se utilizó el diseño experimental parcelas sub-subdivididas, con tres repeticiones (bloques). Se combinaron dos distancias entre hileras: 60 y 80 cm en la parcela principal, tres distancias entre plantas: 20, 30 y 40 cm en la subparcela e hidrogel: 0 y 5 g/planta en la sub-subparcela. Las variables evaluadas fueron: altura de planta, área foliar, índice de área foliar, tasa de crecimiento del cultivo, número de vainas/planta, longitud de vaina, número de semillas/vaina, peso de cien semillas y rendimiento de semillas/ha. El mayor rendimiento, se estimó con 20 cm entre plantas y 60 cm entre hileras (83.333 plantas ha-1), con un rendimiento de 2.027 kg ha-1, asociado a mayor área foliar, índice de área foliar y tasa de crecimiento del cultivo y, a menor número de vainas por planta, longitud de vaina y peso de cien semillas.


ABSTRACT Cowpea bean is an important legume in the Caribbean region of Colombia, for its contribution to the diet of its habitants and being an alternative income for small producers. Nevertheless, the yields obtained do not exceed 600 kg ha-1, due to the unknown of the effect of cultural methods on the species, as in the different cultivars. The purpose of the study was to evaluate the effect of different spacing and hydrogel on vegetative and reproductive characteristics of cowpea beans, cultivar Caupicor 50 in the department of Sucre of the Caribbean region of Colombia. A split-plot design was used, with three replications (blocks). Two distances between rows: 60 and 80 cm in the main plot, three spaces between plants: 20, 30, and 40 cm in the subplot, and hydrogel: 0 and 5 g / plant in the sub-sub plot were combined. The variables evaluated were: plant height, leaf area, leaf area index, crop growth rate, number of pods per plant, pod length, number of seeds per pod, the weight of one hundred seed, and seed yield/ha. The highest yield, 2,027 kg ha-1, was estimated with 20 cm between plants and 60 cm between rows (83,333 plants ha-1) and was associated with greater leaf area, leaf area index and crop growth rate, and a lower number of pods per plant, pod length and weight of one hundred seeds.

3.
Rev. peru. biol. (Impr.) ; 28(4): e21227, Oct.-Dec 2021. tab, graf
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1361075

ABSTRACT

Resumen En la Amazonía colombiana, los insectos constituyen un recurso natural abundante, el cual es aprovechado mediante el consumo de especies comestibles. Históricamente, los pueblos indígenas son entomofágicos y han incluido a insectos y otros artrópodos en sus dietas. En el presente estudio, se analiza el uso tradicional de insectos comestibles en las comunidades del oriente amazónico de Colombia. Durante el período de agosto 2020 a marzo 2021, se realizaron entrevistas abiertas y semi-estructuradas a habitantes del municipio de Mitú (Vaupés) y de 41 comunidades indígenas aledañas, las cuales indagaron sobre la percepción, aprovechamiento y comercialización de insectos comestibles. De acuerdo con su valor de uso, Rhynchophorus palmarum (Coleoptera: Curculionidae), Syntermes spinosus (Blattodea: Termitidae), Atta laevigata (Hymenoptera: Formicidae), Lusura chera (Lepidoptera: Notodontidae) y Polybia liliacea (Hymenoptera: Vespidae), constituyen las especies de insectos comestibles más representativas de la región. Para cada especie se estableció su calendario ecológico y se documentaron sus técnicas y métodos de recolecta, sus formas tradicionales de preparación y consumo, y sus actividades de comercialización. Se resalta el potencial de las especies de insectos comestibles analizadas para el fortalecimiento de estrategias de uso sostenible direccionadas a mejorar la seguridad alimentaria de la población y el mantenimiento de los conocimientos tradicionales de las comunidades.


Abstract In the Colombian Amazon, insects are an abundant natural resource, and part of the use of this resource is the consumption of some edible species. Historically, indigenous peoples are entomophagic and have included insects and other arthropods in their diets. In this paper, the traditional use of edible insects in the communities of the eastern Amazon of Colombia is analyzed. During August 2020 to March 2021, opened and semi-structured interviews carried out with inhabitants of the municipality of Mitú (Vaupés) and 41 surrounding indigenous communities were applied, inquiring about the perception, use and commercialization of edible insects. According to its use value, Rhynchophorus palmarum (Coleoptera: Curculionidae), Syntermes spinosus (Blattodea: Termitidae), Atta laevigata (Hymenoptera: Formicidae), Lusura chera (Lepidoptera: Notodontidae), and Polybia liliacea (Hymenoptera: Vespidae), are the most representative species of edible insects in the region. For each species, its ecological calendar was established, and its collecting techniques and methods, traditional forms of preparation and consumption, and marketing activities were documented. The potential of the edible insect species documented is highlighted to promote sustainable use strategies, with the aim of improving the food security of the population and maintaining the traditional knowledge of these communities.

4.
Arq. bras. med. vet. zootec. (Online) ; 73(4): 781-790, Jul.-Aug. 2021. tab, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1285278

ABSTRACT

The objective of the present study was to Standardize a Polymerase Chain Reaction (PCR) protocol for the authentication of bovine and buffalo milk, and to detect the presence of Salmonella spp. and Listeria monocytogenes. For this, the target DNA was extracted, mixed, and subjected to a PCR assay. Milk samples were defrauded and experimentally contaminated with microorganisms to assess the detection of target DNA at different times of cultivation, bacterial titers, and concentration of genetic material. In addition, the protocol was tested with DNA extracted directly from food, without a pre-enrichment step. The proposed quadruplex PCR showed good accuracy in identifying target DNA sequences. It was possible to simultaneously identify all DNA sequences at the time of inoculation (0h), when the samples were contaminated with 2 CFU/250mL and with 6h of culture when the initial inoculum was 1 CFU/250mL. It was also possible to directly detect DNA sequences from the food when it was inoculated with 3 CFU/mL bacteria. Thus, the proposed methodology showed satisfactory performance, optimization of the analysis time, and a potential for the detection of microorganisms at low titers, which can be used for the detection of fraud and contamination.


O objetivo do presente estudo foi padronizar um protocolo de reação em cadeia da polimerase (PCR) para a autenticação de leite bovino e bubalino e a detecção da presença de Salmonella spp. e Listeria monocytogenes. Para isso, o DNA-alvo foi extraído, misturado e submetido ao ensaio de PCR. Amostras de leite foram fraudadas e contaminadas experimentalmente com os micro-organismos, para se avaliar a detecção do DNA-alvo em diferentes tempos de cultivo, os títulos bacterianos e a concentração de material genético. Além disso, o protocolo foi testado com DNA extraído diretamente do alimento, sem a etapa de pré-enriquecimento. A PCR quadriplex proposta mostrou boa precisão na identificação de sequências de DNA-alvo. Foi possível identificar simultaneamente todas as sequências de DNA no momento da inoculação (0h), quando as amostras estavam contaminadas com 2 UFC/250mL, e com seis horas de cultura, quando o inóculo inicial foi de 1 UFC/250mL. Também foi possível detectar diretamente as sequências de DNA do alimento quando este foi inoculado com 3 UFC/mL de bactérias. Dessa forma, a metodologia proposta apresentou desempenho satisfatório, otimização do tempo de análise e potencial para detecção de micro-organismos em baixos títulos, podendo ser utilizada para detecção de fraude e contaminação.


Subject(s)
Animals , Cattle , Salmonella/isolation & purification , Buffaloes , Milk/microbiology , Fraud/prevention & control , Listeria monocytogenes/isolation & purification , Food Safety/methods , Multiplex Polymerase Chain Reaction/veterinary
5.
Vitae (Medellín) ; 28(3)2021-08-11. Ilustraciones
Article in English | LILACS, COLNAL | ID: biblio-1363266

ABSTRACT

BACKGROUND: Biosensing techniques have been the subject of exponentially increasing interest due to their performance advantages such as high selectivity and sensitivity, easy operation, low cost, short analysis time, simple sample preparation, and real-time detection. Biosensors have been developed by integrating the unique specificity of biological reactions and the high sensitivity of physical sensors. Therefore, there has been a broad scope of applications for biosensing techniques, and nowadays, they are ubiquitous in different areas of environmental, healthcare, and food safety. Biosensors have been used for environmental studies, detecting and quantifying pollutants in water, air, and soil. Biosensors also showed great potential for developing analytical tools with countless applications in diagnosing, preventing, and treating diseases, mainly by detecting biomarkers. Biosensors as a medical device can identify nucleic acids, proteins, peptides, metabolites, etc.; these analytes may be biomarkers associated with the disease status. Bacterial food contamination is considered a worldwide public health issue; biosensor-based analytical techniques can identify the presence or absence of pathogenic agents in food. OBJECTIVES: The present review aims to establish state-of-the-art, comprising the recent advances in the use of nucleic acid-based biosensors and their novel application for the detection of nucleic acids. Emphasis will be given to the performance characteristics, advantages, and challenges. Additionally, food safety applications of nucleic acid-based biosensors will be discussed. METHODS: Recent research articles related to nucleic acid-based biosensors, biosensors for detecting nucleic acids, biosensors and food safety, and biosensors in environmental monitoring were reviewed. Also, biosensing platforms associated with the clinical diagnosis and food industry were included. RESULTS: It is possible to appreciate that multiple applications of nucleic acid-based biosensors have been reported in the diagnosis, prevention, and treatment of diseases, as well as to identify foodborne pathogenic bacteria. The use of PNA and aptamers opens the possibility of developing new biometric tools with better analytical properties. CONCLUSIONS: Biosensors could be considered the most important tool for preventing, treating, and monitoring diseases that significantly impact human health. The aptamers have advantages as biorecognition elements due to the structural conformation, hybridization capacity, robustness, stability, and lower costs. It is necessary to implement biosensors in situ to identify analytes with high selectivity and lower detection limits


ANTECEDENTES: Las técnicas de biodetección han sido objeto de un interés cada vez mayor debido a ventajas, tales como alta selectividad y sensibilidad, facilidad de manejo, bajo costo, tiempo de análisis corto, preparación sencilla de muestras y detección en tiempo real. Los biosensores se han desarrollado integrando la especificidad única de las reacciones biológicas y la alta sensibilidad de los sensores físicos. Por lo tanto, las técnicas de biodetección han tenido un amplio campo de aplicación y hoy en día son omnipresentes en diferentes áreas del medio ambiente, la salud y la seguridad alimentaria. Se han utilizado biosensores para estudios ambientales, detectando y cuantificando contaminantes en el agua, el aire y el suelo. Los biosensores también mostraron un gran potencial para desarrollar herramientas analíticas con innumerables aplicaciones en el diagnóstico, prevención y tratamiento de enfermedades, principalmente mediante la detección de biomarcadores. Los biosensores como dispositivo médico pueden utilizarse para identificar ácidos nucleicos, proteínas, péptidos, metabolitos, etc. Estos analitos pueden ser biomarcadores asociados al estado de la enfermedad. La contaminación bacteriana de los alimentos se considera un problema de salud pública mundial; se pueden utilizar técnicas analíticas basadas en biosensores para determinar la presencia o ausencia de agentes patógenos en los alimentos. OBJETIVOS: La presente revisión tiene por objeto establecer los últimos adelantos en la utilización de biosensores basados en ácidos nucleicos y su novedosa aplicación para la detección de ácidos nucleicos. Se hará hincapié en las características del desempeño, las ventajas y los desafíos. Además, se examinarán las aplicaciones de los biosensores basados en ácidos nucleicos para la inocuidad de los alimentos. MÉTODOS: Se examinaron artículos de investigación recientes relacionados con los biosensores a base de ácidos nucleicos, los biosensores para la detección de ácidos nucleicos, los biosensores y la inocuidad de los alimentos, y los biosensores para la vigilancia del medio ambiente. También se incluyeron plataformas de biosensores asociadas al diagnóstico clínico y a la industria alimentaria. RESULTADOS: Es posible apreciar que se han reportado múltiples aplicaciones de biosensores basados en ácido nucleico para el diagnóstico, prevención y tratamiento de enfermedades, así como para identificar bacterias patógenas transmitidas por los alimentos. El uso de PNA y aptámeros abre la posibilidad de desarrollar nuevas herramientas biométricas con mejores propiedades analíticas. CONCLUSIONES: Los biosensores pueden ser considerados como los instrumentos más importantes para la prevención, el tratamiento y la vigilancia de las enfermedades que tienen un impacto significativo en la salud humana. Los aptámeros tienen ventajas como elemento de biorreconocimiento debido a la conformación estructural, capacidad de hibridación, robustez, estabilidad y menores costos. Es necesario implementar biosensores in situ para identificar analitos con alta selectividad y menores límites de detección


Subject(s)
Humans , Biosensing Techniques , Nucleic Acids , Biomarkers , Diagnosis , Noxae
6.
Diaeta (B. Aires) ; 39(175): 13-24, abr. 2021. graf
Article in Spanish | LILACS, BINACIS, UNISALUD | ID: biblio-1360383

ABSTRACT

Resumen Introducción: los tradicionales estilos de vida y alimentación campesina se han venido reconfigurando por la influencia de la vida urbana, ya no tan lejana; por lo que resulta de interés conocer sus actuales características y garantías que ofrece sobre los niveles de seguridad alimentaria en hogares rurales y periurbanos del Oriente venezolano. Materiales y método: se ha realizado un estudio descriptivo transversal con una muestra de 195 familias, a través de una encuesta que contemplaba datos sociodemográficos, frecuencia habitual de ingesta de alimentos y preparaciones que constituyen las comidas principales, autoproducción domiciliaria, así como la percepción de experiencias de falta de alimentos en el hogar. Resultados: socioeconómicamente el 14,9% de las familias estudiadas se caracterizaron como no pobres, 68,2% en pobreza relativa y un 16,9% en pobreza crítica. El 57,9%, estructuran su dieta en base a la combinación de tres comidas principales diarias y un 94,8% de las familias poseían espacios agrícolas productivos activos en sus domicilios. El 25,1% de la muestra se encontraba en inseguridad alimentaria severa, 18,9% en moderada y el 20,5% en leve. Conclusión: existe inseguridad alimentaria en los sectores periurbano y rurales estudiados, que se agudiza en los estratos socioeconómicos más bajos. La reducción de la inseguridad alimentaria debe priorizar y promover en los hogares estudiados la producción y consumo más variado de alimentos estratégicos, para alcanzar una dieta más equilibrada y sustentable con los recursos locales.


Abstract Introduction: traditional peasant lifestyles and food have been reconfigured by the influence of urban life, not so far away; therefore, it is of interest to know its current characteristics and guarantees that it offers on the levels of food security in rural and peri-urban households in eastern Venezuela. Materials and method: a cross-sectional descriptive study was carried out with a sample of 195 families, through a survey that included sociodemographic data, habitual frequency of food intake and preparations that constitute the main meals, as well as the perception of experiences of lack of food in the home. Results: socioeconomically, 14.87% of the families studied were characterized as non-poor, 68.2% in relative poverty and 16.9% in critical poverty. 57.9% structured their diet based on the combination of three main daily meals and 94.8% of the families had active productive agricultural spaces in their homes. 25.1% of the sample was severely food insecure, 18.9% moderate and 20.5% mild. Conclusion: there is Food Insecurity in the peri-urban and rural sectors studied, and this is linked to poverty; in the lower socioeconomic strata this situation is exacerbated. The reduction of food insecurity should prioritize and promote in the studied households the production and more varied consumption of strategic foods, to achieve a more balanced and sustainable diet with local resources.


Subject(s)
Food Supply , Rural Population , Eating , Food , Life Style
7.
Arq. bras. med. vet. zootec. (Online) ; 73(2): 487-494, Mar.-Apr. 2021. tab
Article in English | LILACS, VETINDEX | ID: biblio-1248939

ABSTRACT

The aim of this study was to evaluate the influence of different periods of pre-slaughter fasting (F1: 2 to 24 hours and F2: 48 to 72 hours) on the counts of hygiene indicator microorganisms and the presence of Salmonella spp. in carcasses of bullfrogs. Two different stages of the slaughter process were analyzed: after bleeding (A) and after the final carcasses cleaning (B). Samples from each fasting period were analyzed to count hygiene indicator microorganisms (n=30) and Salmonella spp. (n=140). For aerobic mesophilic microorganisms, the variation in fasting periods caused a reduction of 0.69 log10 CFU / g (P<0.05) in F2 when compared to F1 at point B of the slaughter. Coliforms at 35º C and Escherichia coli showed no differences (P >0.05) between the fasting analyzed periods. Considering the presence of E. coli, it was observed that F2 resulted in a reduction of 30% (P<0.05) positivity on point B. For Salmonella spp., the results showed that F2 contributed to an 11.5% reduction in the presence of this bacteria at point B. (P<0.05). Therefore, it is concluded that 48 to 72 hours of pre-slaughter fasting resulted in a positive impact on the microbiological quality of bullfrog carcasses.(AU)


O objetivo deste estudo foi avaliar a influência de diferentes períodos de jejum pré-abate (F1: duas a 24 horas e F2: 48 a 72 horas) nas contagens de micro-organismos indicadores de higiene e na presença de Salmonella spp. em carcaças de rãs-touro. Foram analisadas duas etapas do processo de abate: após a sangria (A) e após a toalete final da carcaça (B). As amostras de cada período de jejum foram utilizadas para contagem de indicadores de higiene (n = 30) e Salmonella spp. (n = 140). Para aeróbios mesófilos, a variação no tempo de jejum causou uma redução de 0,69 log10 UFC/g (P<0,05) em F2 quando comparado a F1 na etapa B do abate. Os coliformes a 35ºC e Escherichia coli não apresentaram diferenças (P>0,05) entre os dois períodos de jejum analisados. Considerando a presença de E. coli, F2 resultou em uma redução de 30% (P<0,05) de positividade na etapa B. Para Salmonella spp., os resultados mostraram que F2 contribuiu para uma redução de 11,5% na presença desse micro-organismo na etapa B. Portanto, conclui-se que 48 a 72 horas de jejum pré-abate tiveram um impacto positivo na qualidade microbiológica das carcaças de rã-touro.(AU)


Subject(s)
Animals , Rana catesbeiana/microbiology , Salmonella/isolation & purification , Food Hygiene , Escherichia coli/isolation & purification , Food Safety , Fasting , Animal Culling
8.
Semina cienc. biol. saude ; 42(1): 29-36, jan./jun. 2021. Tab
Article in English | LILACS | ID: biblio-1247833

ABSTRACT

The population interest for fish consumption has increased, mainly due to several beneficial nutritional properties presented by this food. In this context, oriental culinary also brings different eating habits as consume raw food, such as sashimi. A relevant food contaminant of fecal origin is Escherichia coli, able to become potentially harmful, when it acquires virulence factors, as Shiga toxin-producing E. coli (STEC). This study aimed to evaluate 30 samples of salmon sashimi regarding the presence of E. coli, as well as perform the genotypic characterization of virulence factors associated with STEC. Three samples were collected from 10 different restaurants, specialized in Japanese culinary in the city of Londrina - PR. The E. coli identification was performed using the Colilert® chromogenic substrate technique and biochemical tests, and for the investigation of virulence genes, stx1 and stx2, the polymerase chain reaction (PCR) was used. Among the 30 samples analyzed, 15 (50%) presented contamination by E. coli. However, in no sample were detected virulence factors associated with STEC. Although human diseases associated with STEC are poorly described in Brazil, it is possible to verify that fish, mainly those consumed raw, are potential transmitters of E. coli to humans. This can compromise the food safety of these products and, thus, characterize them as unsuitable for consumption. Therefore, it is necessary the adoption of preventive measures of contamination by E. coli in products intended to human consumption, beyond more research that can verify the potential of STEC as a fish contaminant. (AU)


O interesse da população pelo consumo de peixe tem aumentado, principalmente devido às diversas propriedades nutricionais benéficas apresentadas por este alimento. Neste contexto, a culinária oriental também traz diferentes hábitos alimentares, como consumir alimentos crus, tais como o sashimi. Um relevante contaminante alimentar de origem fecal é Escherichia coli, capaz de tornarse potencialmente danosa ao adquirir fatores de virulência, como E. coli produtora de toxina Shiga (STEC). Este estudo objetivou avaliar 30 amostras de sashimi de salmão quanto à presença de E. coli, bem como realizar a caracterização genotípica de fatores de virulência associados com STEC. Três amostras foram coletadas de 10 diferentes restaurantes especializados em culinária japonesa da cidade de Londrina - PR. A identificação de E. coli foi realizada utilizando a técnica de substrato cromogênico Colilert® e testes bioquímicos, e para a investigação dos genes de virulência, stx1 e stx2, a reação em cadeia da polimerase (PCR) foi utilizada. Dentre as 30 amostras analisadas, 15 (50%) apresentaram contaminação por E. coli. Contudo, em nenhuma das amostras foram detectados fatores de virulência associados com STEC. Embora as doenças humanas associadas com STEC sejam pouco descritas no Brasil, é possível verificar que os peixes, principalmente aqueles consumidos crus, são potenciais transmissores de E. coli aos humanos. Isto pode comprometer a segurança alimentar destes produtos e, assim, caracterizá-los como impróprios para o consumo. Portanto, é necessária a adoção de medidas preventivas de contaminação por E. coli nos produtos destinados ao consumo humano, além de mais pesquisas que possam verificar o potencial de STEC como um contaminante de peixes. (AU)


Subject(s)
Humans , Restaurants , Salmon , Escherichia coli , Shiga-Toxigenic Escherichia coli , Raw Foods , Food
9.
Semina cienc. biol. saude ; 42(1): 71-80, jan./jun. 2021. Tab
Article in Portuguese | LILACS | ID: biblio-1247939

ABSTRACT

O mel de abelha é um produto de consumo relevante pela população devido aos seus fatores nutricionais e terapêuticos. A adulteração é um exemplo de como as características do mel podem ser afetadas, prejudicando os consumidores que desejam comprar produtos puros e de alta qualidade. Este trabalho teve como objetivo avaliar a qualidade físico-química do mel de abelha comercializado em Brasília, Distrito Federal. Foram obtidas 13 amostras de mel de abelhas: uma de um apicultor da região com certificação de produção (usada como controle negativo), seis de lojas de produtos naturais e hipermercados no centro de Brasília e seis de feira livre e pequenos estabelecimentos de uma região administrativa do Distrito Federal, das quais cinco não tinham o selo do Serviço de Inspeção Federal (SIF) na embalagem. Também foi usada uma amostra comercial de xarope de glicose como controle positivo. Para verificar a adulteração, foram adotados os testes de Lund, Fiehe e Lugol, bem como as análises de pH, acidez titulável e umidade, para serem comparados com os parâmetros de qualidade do mel estabelecidos pela legislação vigente. As análises verificaram a qualidade dos méis comercializados com o selo SIF, com algumas ressalvas para a possível alteração de temperatura. Já as que não possuíam selo, apenas uma foi aprovada nos testes de qualidade, porém sua venda não é regularizada. A maioria das amostras analisadas apresentaram resultados consistentes com os padrões exigidos pela legislação vigente, com maiores irregularidades na comercialização de méis em feira livre, mostrando a necessidade de maior controle de qualidade e inspeção de produtos não certificados.(AU)


Bee honey is a product of relevant consumption by the population due to its nutritional and therapeutic factors. Tampering is an example of how honey characteristics can be affected and harming consumers who want to buy a high quality and pure product. This work aimed to evaluate the physicochemical quality of bee honey commercialized in Brasília, Federal District. Thirteen samples of bee honey were obtained: one from a certified local beekeeper (used as a negative control), six from natural products and hypermarkets in central Brasília and six from street fair and small establishments in one of the administrative region of the Federal District, of which five did not have the seal of the Federal Inspection Service (SIF) on the packaging. Also, a sample of commercial glucose syrup was used as positive control. To verify tampering, the Lund, Fiehe and Lugol tests were adopted, as well as the pH, titratable acidity and humidity analysis, to be compared honey quality parameters established by the current legislation. The analyzes verified the quality of the honeys sold with SIF seal, with some restraints for the possible temperature changes. As for those that did not have a seal, only one passed the quality tests, but its sale is not regularized. Most of the samples analyzed showed results consistent with the standards requirements of the current legislation, with greater irregularities in the marketing of honey in street fair, showing the need for greater quality control and inspection of non-certified products.(AU)


Subject(s)
Animals , Bees , Quality Management , Honey , Quality Control , Product Packaging
10.
Article in English | WPRIM | ID: wpr-922194

ABSTRACT

Antimicrobial resistance (AMR) is increasingly becoming a threat to global public health, not least in low- and middle-income countries (LMICs) where it is contributing to longer treatment for illnesses, use of higher generation drugs, more expenditure on antimicrobials, and increased deaths attributed to what should be treatable diseases. Some of the known causes of AMR include misuse and overuse of antimicrobials in both humans and animals, unnecessary use of antimicrobials in animals as growth promoters, and lack of awareness among the public on how to protect antimicrobials. As a result, resistant organisms are circulating in the wider environment, and there is a need to consider the One Health approach to minimise the continuing development of AMR. Environmental Health, specifically water, sanitation and hygiene (WASH), waste management, and food hygiene and safety, are key components of One Health needed to prevent the spread of antimicrobial-resistant microorganisms particularly in LMICs and reduce the AMR threat to global public health. The key Environmental Health practices in the prevention of AMR include: (1) adequate WASH through access and consumption of safe water; suitable containment, treatment and disposal of human excreta and other wastewater including from health facilities; good personal hygiene practices such as washing hands with soap at critical times to prevent the spread of resistant microorganisms, and contraction of illnesses which may require antimicrobial treatment; (2) proper disposal of solid waste, including the disposal of unused and expired antimicrobials to prevent their unnecessary exposure to microorganisms in the environment; and (3) ensuring proper food hygiene and safety practices, such as sale and consumption of animal products in which adequate antimicrobial withdrawal periods have been observed, and growing vegetables on unpolluted soil. Environmental Health is therefore crucial in the prevention of infectious diseases that would require antimicrobials, reducing the spread of resistant organisms, and exposure to antimicrobial residues in LMICs. Working with other professionals in One Health, Environmental Health Practitioners have a key role in reducing the spread of AMR including health education and promotion, surveillance, enforcement of legislation, and research.


Subject(s)
Developing Countries , Disease Transmission, Infectious/prevention & control , Drug Resistance, Microbial , Environmental Health/standards , Food Safety , Health Personnel/standards , Humans , Hygiene/standards , Role , Sanitation/standards , Waste Management/standards
11.
Ciênc. rural (Online) ; 51(11): e20200967, 2021. tab, graf
Article in English | LILACS-Express | LILACSEXPRESS, LILACS, VETINDEX | ID: biblio-1278891

ABSTRACT

ABSTRACT: Buffalo milk is rich in nutrients and can serve as a substrate for the proliferation of microorganisms. Thus, the objective of the present study was to evaluate the growth kinetics of Salmonella Typhimurium and Listeria monocytogenes in buffalo milk under different processing and storage conditions. Samples of raw and pasteurized milk were inoculated with 1 CFU of each bacterium, separately and together, per 25 mL. After contamination, samples were stored at 8 °C or 37 °C, and bacterial counts were performed at 24, 48, and 168 h. In addition, the accompanying microbiota growth, pH, and the effect of these variables on the growth kinetics of microorganisms were monitored. The pathogens tested were able to proliferate under most conditions tested, reaching high titers throughout the experimental period. At 37 °C, there was a decrease in pH and an increase in the accompanying microbiota that interfered with the microbial growth curve. It was also observed that pasteurized milk subjected to 8 °C provided better conditions for the multiplication of bacteria. Therefore, it was concluded that care throughout the production chain, storage, and commercialization of milk must be adopted to guarantee the microbiological safety of this food.


RESUMO: O leite bubalino é rico em nutrientes e pode servir de substrato para a proliferação de micro-organismos. Assim, o objetivo do presente estudo foi avaliar a cinética de crescimento de Salmonella Typhimurium e Listeria monocytogenes em leite bubalino, em diferentes condições de processamento e armazenamento. Para isso, utilizaram-se amostras de leite cru e pasteurizado, que foram inoculadas com 1 UFC/25 mL de cada bactéria separadamente e em conjunto. Após a contaminação, as amostras foram armazenadas nas temperaturas de 8 ºC e 37 ºC e realizadas contagens bacterianas em 24, 48 e 168h. Além disso, foi acompanhado o crescimento da microbiota acompanhante, o pH e o efeito dessas variáveis sobre a cinética de crescimento dos micro-organismos. Os patógenos testados conseguiram se proliferar na maioria das condições testadas, atingindo altos títulos durante todo o período experimental. Na temperatura de 37 ºC, houve uma diminuição do pH e um aumento da microbiota acompanhante, o que interferiu na curva de crescimento microbiana. Observou-se também que o leite pasteurizado e submetido a 8 ºC possibilitou melhores condições para a multiplicação das bactérias. Sendo assim, concluiu-se que cuidados durante toda a cadeia de produção, armazenamento e comercialização do leite devem ser adotados para garantir a segurança microbiológica desse alimento.

12.
Article in Chinese | WPRIM | ID: wpr-881474

ABSTRACT

Since July 2020, nucleic acids of novel coronavirus SARS-CoV-2 were frequently detected in cold chain food imported to China, making imported food being a potential risk to the COVID-19 outbreak. Based on the epidemiological characteristics of SARS-CoV-2, imported cold-chain food and their packaging may become the carriers of SARS-CoV-2 in long-distance cross-border transportation. In each aspect of stakeholders including governmental supervision, manufacturers and consumers, China should strengthen the risk management of imported cold-chain food to ensure the safety, especially pay attention to the health protection of industry workers in close contact with cold -chain food.

13.
Article in Chinese | WPRIM | ID: wpr-881473

ABSTRACT

Objective:To determine the current situation of food safety risk monitoring ability of centers for disease control and prevention (CDCs) in a province, and provide reference for strengthening the construction of food safety monitoring ability in the future. Methods:A self-administered questionnaire survey was conducted among 14 CDCs who are responsible for food safety risk monitoring in the province in 2018. Results:The ratio of food inspection staff with senior, middle, and primary professional titles was 1∶1∶1. The educational background of food inspection staff at the provincial and municipal levels was mainly bachelor degree or above, while that of county level directly under the administration of the province was mainly college degree. The age composition of the staff at the provincial level tended to be elder; however, there were more staff with less working experience at the county level. The qualification proportion of laboratory area was 50%. The distribution proportion of food inspection equipment in the CDC at all levels was 83.3%, 79.5% and 36.3%, respectively. In total, 14 CDCs completed the monitoring task as required. They were all accredited with laboratory qualifications; however, one CDC did not conduct re-evaluation after the certification of food inspection qualification expired. Each CDC ensured the accuracy and reliability of monitoring data through the supervision in food testing. Conclusion:The food safety risk monitoring ability of the CDCs in the province is continuously improved, which can meet the needs of routine monitoring. However, the composition of professional titles of food inspection staff is not very reasonable. There is still a gap between the equipment configuration and the national requirements. The ability of food inspection needs to be improved, in which quality management and laboratory qualification should be further strengthened.

14.
Article in English | WPRIM | ID: wpr-880373

ABSTRACT

BACKGROUND@#Food safety and hygiene are currently a global health apprehension especially in unindustrialized countries as a result of increasing food-borne diseases (FBDs) and accompanying deaths. This study aimed at assessing knowledge, attitude, and hygiene practices (KAP) of food safety among street-cooked food handlers (SCFHs) in North Dayi District, Ghana.@*METHODS@#This was a descriptive cross-sectional study conducted on 407 SCFHs in North Dayi District, Ghana. The World Health Organization's Five Keys to Safer Food for food handlers and a pretested structured questionnaire were adapted for data collection among stationary SCFHs along principal streets. Significant parameters such as educational status, average monthly income, registered SCFHs, and food safety training course were used in bivariate and multivariate logistic regression models to calculate the power of the relationships observed.@*RESULTS@#The majority 84.3% of SCFHs were female and 56.0% had not attended a food safety training course. This study showed that 67.3%, 58.2%, and 62.9% of SCFHs had good levels of KAP of food safety, respectively. About 87.2% showed a good attitude of separating uncooked and prepared meal before storage. Good knowledge of food safety was 2 times higher among registered SCFHs compared to unregistered [cOR=1.64, p=0.032]. SCFHs with secondary education were 4 times good at hygiene practices of food safety likened to no education [aOR=4.06, p=0.003]. Above GHc1500 average monthly income earners were 5 times good at hygiene practices of food safety compared to below GHc500 [aOR=4.89, p=0.006]. Registered SCFHs were 8 times good at hygiene practice of food safety compared to unregistered [aOR=7.50, p<0.001]. The odd for good hygiene practice of food safety was 6 times found among SCFHs who had training on food safety courses likened to those who had not [aOR=5.97, p<0.001].@*CONCLUSIONS@#Over half of the SCFHs had good levels of KAP of food safety. Registering as SCFH was significantly associated with good knowledge and hygiene practices of food safety. Therefore, our results may present an imperative foundation for design to increase food safety and hygiene practice in the district, region, and beyond.


Subject(s)
Adult , Attitude , Cooking/methods , Cross-Sectional Studies , Female , Food Safety , Ghana , Health Knowledge, Attitudes, Practice , Humans , Hygiene , Male , Middle Aged , Socioeconomic Factors , Young Adult
15.
Chinese Journal of Biotechnology ; (12): 378-383, 2021.
Article in Chinese | WPRIM | ID: wpr-878568

ABSTRACT

In recent years, to solve the increasingly prominent problem of the contradiction between human social development and environmental resources, artificial meat has appeared in public view more and more. Generally speaking, the artificial meat can be divided into vegetable protein meat and cell cultured meat. Among them, vegetable protein meat has gradually begun to be commercialized, and cell cultured meat is cultured with animal cells, which is more similar to the real meat. Based on the analysis of the essence of cell cultured meat, we explore the positive significance of cell cultured meat technology for the meat production industry, consumer groups, and the sustainable development of mankind in the future. From the perspective of bioethics, the research, development and production of cell cultured meat can help ensure the sustainable development of human society, improve animal welfare, reduce resource demand, improve the nutritional function of meat products, and provide new growth points for the development of other industries. In addition, the ethical risks of food safety, technology abuse and technical supervision involved in cell cultured meat production are put forward for deep consideration, hoping to provide reference for the sustainable development of artificial meat industry from the perspective of bioethics.


Subject(s)
Animal Welfare , Animals , Food Safety , Humans , Meat , Meat Products
16.
Article in Chinese | WPRIM | ID: wpr-877085

ABSTRACT

Objective To analyze the epidemiological characteristics of food poisoning in Shenzhen from 2009 to 2018, and to provide a scientific basis for formulating control strategies of food poisoning. Methods The data of food poisoning events in Shenzhen from 2009 to 2018 was analyzed with descriptive epidemiological methods. Results There were 182 reported food poisoning events, resulting in 1786 cases and 3 death. The incidence of food poisoning events had a seasonal peak during May to September, and a total of 119 reported food poisoning events (65.39%, 119/182) occurred in that period of time with 1156 cases (64.73%, 1156/1786). There were 77 food poisoning events (42.31%) occurring in collective canteens with 927 cases (51.91%), making them the most frequent locations for food poisoning. The main pathogenic factors were bacteria, poisonous plants and chemicals. Incidents of bacterial food poisoning were mainly caused by Vibro parahemolyticus (51.73%, 60/116), Salmonella (26.73%, 30/116), and Staphylococcus aureus enterotoxin (11.21%, 13/116), etc. Hyacinth bean toxin and mushroom were responsible for 33.33% of the incidents caused by poisonous plants. Incidents of chemical food poisoning were mainly caused by nitrite (8.33%). Conclusion The prevention and control of food poisoning in Shenzhen should be focused on reducing bacterial food poisoning such as Salmonella and Vibrio parahaemolyticus, and supervision of food safety of catering units and collective canteens should be strengthened. Additionally, health education on prevention and control of food poisoning should be targeted at the high-risk population to reduce the risk of food poisoning.

17.
Rev. bras. parasitol. vet ; 30(2): e029320, 2021. tab
Article in English | LILACS-Express | MEDLINE, LILACSEXPRESS, LILACS, VETINDEX | ID: biblio-1288693

ABSTRACT

Abstract Toxoplasmosis occurs worldwide causing economic losses to the animal production and problems to the public health. The study aimed to detect Toxoplasma gondii and Sarcocystis spp.in 141 meat products from commercial meat cuts of pork, beef, and kibbeh sold in commercial markets from Botucatu, SP, Brazil. Samples were bioassayed in mice to isolate the parasite, and the parasite DNA detected by PCR targeting the 529 base pairs repeat element region (PCR-529-bp). All samples resulted negative on bioassay, whereas PCR positive for 9 (6,38%), distributed as 5/48 beef, 3/49 pork, and 1/44 kibbeh. PCR-positive were investigated for the the parasite genotype using multiplex-, nested-, and RFLP-PCR for 11 markers (SAG1, 5'-3'SAG2, alt.SAG2, SAG3, B-TUB, GRA6, L358, c22-8, c29-6, PK1, Apico). Complete genotype was determined on just one PCR-positive sample that matched MAS, TgCkBr89 and TgCkBr147 isolates already identified. In addition, nested- and RFLP-PCR targeting 18S rRNA was run for all PCR-positive samples and, the products, sequenced and aligned to the GenBank at NCBI website. Four samples showed 100% homology with T. gondii (GenBank #L37415.1), three with Sarcocystis hominis (GenBank #AF006471.1), two Sarcocystis cruzi (GenBank #AF176934.1), and one Sarcocystis hirsuta (GenBank #AF006469.1), indicating the circulation of T. gondii and Sarcocystis spp.


Resumo A toxoplasmose está mundialmente distribuída e causa perdas na produção animal e problemas de saúde pública. Objetivou-se detectar Toxoplasma gondii e Sarcocystis spp. em 141 produtos cárneos de origem suína (49), bovina (48) e de quibe cru (44), comercializados em mercados de Botucatu, SP, Brazil. Realizou-se bioensaio das amostras em camundongos para isolamento do parasita, e detecção do DNA pela reação em cadeia pela polimerase, tendo como alvo a região do elemento repetitivo de 529 pares de bases (PCR-529-bp). Todas as amostras foram negativas ao bioensaio e 9 (6,38%) positivas à PCR, sendo 5/48 bovinas, 3/49 suínas e 1/44 quibe. Determinou-se a genotipagem das amostras positivas pela multiplex-, nested- e RFLP-PCR com 11 marcadores (SAG1, 5'-3'SAG2, alt.SAG2, SAG3, B-TUB, GRA6, L358, c22-8, c29-6, PK1, Apico). Obteve-se genótipo completo em uma amostra, semelhante a outros já identificados (MAS, TgCkBr89 e TgCkBr147). Nested- e RFLP-PCR do gene 18S rRNA das amostras positivas à PCR foram realizadas, e os produtos da nested-PCR, sequenciados e alinhados com dados do GenBank no NCBI. Quatro apresentaram 100% de homologia com T. gondii (L37415.1), duas Sarcocystis hominis (AF006471.1), duas Sarcocystis cruzi (AF176934.1), uma Sarcocystis hirsuta (AF006469.1), indicando a circulação de T. gondii e Sarcocystis spp.

18.
Article in English | WPRIM | ID: wpr-888618

ABSTRACT

BACKGROUND@#Food handlers can play a vital role into reducing foodborne diseases by adopting appropriate food handling and sanitation practices in working plants. This study aimed to assess the factors associated with food safety knowledge and practices among meat handlers who work at butcher shops in Bangladesh.@*METHODS@#A cross-sectional study was conducted among 300 meat handlers from January to March, 2021. Data were collected through in-person interviews using a structured questionnaire. The questionnaire consisted of three parts; socio-demographic characteristics, assessments of food safety knowledge, and food safety practices. A multiple logistic regression model was used to identify the factors associated with food safety knowledge and practices.@*RESULTS@#Only 20% [95% confidence interval, (CI) 15.7-24.7] and 16.3% (95% CI 12.3-20.7) of the respondents demonstrated good levels of food safety knowledge and practices, respectively. The factors associated with good levels of food safety knowledge were: having a higher secondary education [adjusted odds ratio (AOR) = 4.57, 95% CI 1.11-18.76], income above 25,000 BDT/month (AOR = 10.52, 95% CI 3.43-32.26), work experience of > 10 years (AOR = 9.31, 95% CI 1.92-45.09), ≥ 8 h per day of work (AOR = 6.14, 95% CI 2.69-13.10), employed on a daily basis (AOR = 4.05, 95% CI 1.16-14.14), and having food safety training (AOR = 8.98 95% CI 2.16-37.32). Good food safety knowledge (AOR = 5.68, 95% CI 2.33-13.87) and working ≥ 8 h per day (AOR = 8.44, 95% CI 3.11-22.91) were significantly associated with a good level of food safety practice.@*CONCLUSIONS@#Poor knowledge and practices regarding food safety were found among Bangladeshi meat handlers. Findings may help public health professionals and practitioners develop targeted strategies to improve food safety knowledge and practices among this population. Such strategies may include education and sensitization on good food safety practices.


Subject(s)
Adolescent , Adult , Aged , Bangladesh , Female , Food Handling/statistics & numerical data , Food Safety/methods , Health Knowledge, Attitudes, Practice , Humans , Meat , Middle Aged , Young Adult
19.
Rev. Inst. Adolfo Lutz ; 80(Único): e37320, dez. 2021. tab, ilus
Article in English | ColecionaSUS, LILACS, ColecionaSUS, SES-SP, CONASS, SESSP-ACVSES, SESSP-IALPROD, SES-SP, SESSP-IALACERVO | ID: biblio-1368348

ABSTRACT

Freezing is an important strategy to keep fish quality and make the species available the whole year. Its effects on the nutritional value of 17 fish species were studied in samples of entire fish, fillets or pieces. One portion of homogenized flesh was analyzed just after purchase (fresh sample). The other portion was packed in polyethylene bag, sealed, quick frozen (-80°C), stored properly at -18°C and analyzed after 12 months (frozen sample). Moisture, ash and protein content were tested using Brazilian Supply, Livestock and Agriculture Ministry methodologies. Lipid content was analyzed through Bligh and Dyer method. Carbohydrate content and caloric value were calculated, using NIFEXT fraction and Atwater coefficient, respectively. When fresh and frozen samples were compared, moisture and ash content showed significant difference (p<0.05) for 17.65% and 11.77% species, respectively. Lipid and protein contents were the most affected parameters, as they were altered in 29.40% of the studied species (p<0.05), and therefore, highlighted the importance of the conservation technology used on nutritional quality of fishery products. Mullet (M. brasiliensis) and Atlantic salmon (S. salar) had their nutritional composition more affected by freezing process with five and four altered parameters, respectively, from the six studied. (AU)


O congelamento é estratégia importante para manter a qualidade do peixe e tornar inúmeras espécies disponíveis o ano todo. Seus efeitos sobre o valor nutricional de 17 espécies foram estudados em amostras de peixes inteiros, filés ou postas. A porção cárnea homogeneizada foi analisada logo após a aquisição (amostra fresca). Outra parte foi embalada em polietileno, selada, rapidamente congelada (-80°C) e analisada após 12 meses de armazenamento a -18°C (amostra congelada). O teor de umidade, cinzas e proteína foram testados com metodologias do Ministério de Agricultura, Pecuária e Abastecimento e teor de lipídios com método de Bligh e Dyer. Conteúdo de carboidrato e valor calórico foram calculados, utilizando fração NIFEXT e coeficiente de Atwater, respectivamente. Quando se comparou amostras frescas e congeladas, teor de umidade e cinzas evidenciaram diferenças significativas (p<0,05) para 17,65% e 11,77% das espécies, respectivamente. O teor de lipídios e de proteínas foram alterados em 29,40% das espécies estudadas (p<0,05), sendo os parâmetros mais afetados pelo congelamento e destacaram a importância da tecnologia de conservação utilizada sobre a qualidade nutricional do pescado. Tainha (M. brasiliensis) e salmão (S. salar) foram as mais afetadas pelo congelamento, com 5 e 4 parâmetros alterados, respectivamente, após estocagem sob congelamento. (AU)


Subject(s)
Animals , Food Quality , Fishes , Food Preservation/methods , Freezing , Food Supply , Nutritive Value
20.
Rio de Janeiro; s.n; 2021. 111 f p. tab, il.
Thesis in Portuguese | LILACS | ID: biblio-1370672

ABSTRACT

A história do combate à fome e da constituição de um campo de ação política como a Segurança Alimentar e Nutricional (SAN) no contexto brasileiro guarda algumas particularidades. Um ator que sempre é citado como central nesse processo é o Fórum Brasileiro de Soberania e Segurança Alimentar e Nutricional (FBSSAN), no entanto, embora o Fórum já tenha mais de vinte anos de trajetória, até o momento, não foi feito nenhum registro desse grupo que permita compreender, além da história, sua atuação política ao longo dos anos. O propósito deste trabalho foi analisar o processo de formação e atuação do FBSSAN, com ênfase na década de 90, buscando compreender como se deu sua incidência na consolidação da SAN no país. Para alcançá-lo, traçamos os seguintes objetivos específicos: caracterizar o contexto histórico, mapear quais foram os atores envolvidos nesse processo e analisar quais foram os principais argumentos que deram sustentação para a criação do Fórum e seus desdobramentos. Foram analisados, ao menos, 63 documentos provenientes de acervos pessoais, bem como realizadas sete entrevistas com atores-chave desse processo. Foi possível identificar que a atuação do Fórum, desde a origem, foi concentrada em um pequeno núcleo de pessoas residentes no eixo Rio-São Paulo, uma baixa renovação de quadros. Em relação à agenda, a eleição de temas prioritários era pautada pelo contexto da política nacional, ou seja, inicialmente, a agenda era mais estrutural na disputa pela institucionalização de leis, diretrizes, políticas, passando, posteriormente, a ser voltada para a criação de dispositivos de monitoramento, avaliação e fiscalização. Em cenários desfavoráveis à participação social, o FBSSAN ganha centralidade, uma vez que passa a ser uma das principais referências para a mobilização social da SAN. A multiplicidade de atores refletiu na construção de um arcabouço conceitual que contemplou a intersetorialidade que o tema exigia. Por fim, fica evidente o importante papel exercido pelo Fórum, os desafios que impõe estão intimamente relacionados à renovação e a uma atualização de formas de se comunicar e seguir mobilizando-se.


The history of the fight against hunger and the constitution of a field of political action such as Food and Nutritional Security (FNS) in the Brazilian context has some particularities. One actor that is always mentioned as central in this process is the Fórum Brasileiro de Soberania e Segurança Alimentar e Nutricional (Brazilian Forum for Food and Nutritional Sovereignty and Security - FBSSAN). The purpose of this work was to analyze the process of formation and the operation of the FBSSAN, with emphasis on the 90s, seeking to understand how its incidence on the consolidation of SAN in the country occurred. To achieve this, we set the following specific objectives: characterize the historical context, map the actors involved in this process, and analyze the main arguments that supported the creation of the Forum and its developments. At least 63 documents from personal collections were analyzed, as well as seven interviews with key players in this process. It was possible to identify that the work of the Forum, from its inception, was concentrated on a small nucleus of people residing in the Rio de Janeiro-São Paulo axis, with a low level of renovation. In relation to the agenda, the election of priority themes was guided by the context of national politics. That is, initially the agenda was more structural, in the dispute for the institutionalization of laws, guidelines, and policies, and later became focused on the creation of monitoring, evaluation, and inspection mechanisms. In scenarios unfavorable to social participation, the FBSSAN gained centrality, since it became one of the main references for social mobilization for SAN. The multiplicity of actors reflected in the construction of a conceptual framework that contemplated the intersectoriality that the theme demanded. Finally, it is evident the important role played by the Forum, and the challenges it poses are closely related to the renewal and updating of ways to communicate and continue mobilizing.


Subject(s)
Public Policy , Social Participation , Political Activism , Food Supply
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