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Background: The genetic diversity and stability analysis based on agro-morphological traits among rice genotypes developed through SSR marker-assisted backcrossing was evaluated at college of agricultural science (CAS), Ebonyi State niversity, Abakaliki, Nigeria. Methods: In this study, 9 rice accessions sourced from the Iinstitute of tropical agriculture and food security (ITAFoS), universiti Putra, Malaysia and Ebonyi, Nigeria; were evaluated for yield stability performance. The experiments were laid out in randomized complete block design (RCBD) with three replications each for two seasons. Results: Result of the evaluated rice accessions showed highly marked morphological variability based on the mean performance and were grouped into three main clusters. Cluster I had CP, cluster II contained Line4, Line5, Line6, and Line8, and cluster III had Line1, Line7, Line12, and Putra-1. The first, 3 principal components were identified and retained for having the Eigen-value >1. The first PC had an eigenvalue of (6.30) with 52.5% proportion of which plant height accounted most for this variation with (0.359) whereas panicle length contributed the least (-0.13). For stability analysis, CP had highest values for most of the parameters. High heritability value was recorded for all nine traits which range between 71.04% and 95.06%. The highest heritability value (95.06%) was found in Days 50% heading while grain width had the lowest (71.04%). Conclusions: It could be concluded that CP, Putral-1, and line7 were the most stable in terms of yield across the two seasons of this study; hence, recommended for parental materials for hybridization.
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Introducción: El desarrollo de vacunas seguras y eficaces contra el SARS-CoV-2 supuso un enorme reto para enfrentar la pandemia de la COVID-19. La aparición de nuevas variantes del SARS-CoV-2 representa un reto en la evaluación de la efectividad de las vacunas, diferentes candidatos vacunales y terapéuticos desarrollados por la comunidad científica. Objetivos: Caracterizar la diversidad genética de aislamientos virales cubanos en el periodo comprendido entre junio de 2020 y diciembre de 2022. Métodos: Se obtuvo el ARN de SARS-CoV-2 de 27 aislamientos a partir de sobrenadante de cultivo celular y se secuenció el gen S. Las secuencias generadas se emplearon para la identificación y posterior caracterización molecular de las variantes genéticas del virus mediante análisis filogenético y el uso de las herramientas disponibles en la base de datos GISEAD. Resultados: Las variantes detectadas en los aislamientos cubanos de SARS-CoV-2 estudiados se correspondieron a las identificadas en los estudios de vigilancia genómica realizados en las diferentes etapas pandémicas de la COVID-19 en Cuba. El 33,3 % de los aislamientos secuenciados correspondieron a los diferentes linajes de la variante Ómicron, seguido de la variante Beta B 1.351 (29,6 %), otros linajes de SARS-CoV-2 (25,9 %), Alfa B 1.1.7 (7,4 %) y Delta B.1.575 (3,7 %). Se detectó la mutación D614G en todos los aislamientos de SARS-CoV-2 estudiados. Conclusiones: La caracterización molecular de los aislamientos cubanos de SARS-CoV-2 tiene una elevada diversidad genética. Posibilita evaluar in vitro e in vivo los candidatos vacunales y agentes terapéuticos desarrollados por la industria biofarmacéutica cubana.
Introduction: The development of safe and effective vaccines against SARS-CoV-2 posed a huge challenge to face the COVID-19 pandemic. The appearance of new variants of SARS-CoV-2 represents a challenge in evaluating the effectiveness of vaccines, different vaccine and therapeutic candidates developed by the scientific community. Objectives: Characterize and analyze the genetic diversity of Cuban viral isolates, in the period between June 2020 and December 2022. Methods: SARS-CoV-2 RNA was obtained from 27 isolates from cell culture supernatant and the S gene was sequenced. The generated sequences were used for the identification and subsequent molecular characterization of the genetic variants of the virus through phylogenetic analysis and the use of the tools available in the GISEAD database. Results: The variants detected in the Cuban SARS-CoV-2 isolates corresponded to those identified in the genomic surveillance studies carried out in the different stages of the COVID-19 pandemic in Cuba. 33.3% of the sequenced isolates corresponded to the different lineages of the Omicron variant, followed by Beta B 1.351 (29.6%), other SARS-CoV-2 lineages (25.9%), Alpha B 1.1.7 (7.4%) and Delta B.1.575 (3.7%). The D614G mutation was detected in all SARS-CoV-2 isolates studied. Conclusions: The molecular characterization of the Cuban isolates of SARS-CoV-2 has a high genetic diversity. It makes it possible to evaluate in vitro and in vivo vaccine candidates and therapeutic agents developed by the Cuban biopharmaceutical industry.
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The elucidation of resources pertaining to the Chimonanthus praecox varieties and the establishment of a fingerprint serve as crucial underpinnings for advancing scientific inquiry and industrial progress in relation to C. praecox. Employing the SSR molecular marker technology, an exploration of the genetic diversity of 175 C. praecox varieties (lines) in the Yanling region was conducted, and an analysis of the genetic diversity among these varieties was carried out using the UPDM clustering method in NTSYSpc 2.1 software. We analyzed the genetic structure of 175 germplasm using Structure v2.3.3 software based on a Bayesian model. General linear model (GLM) association was utilized to analyze traits and markers. The genetic diversity analysis revealed a mean number of alleles (Na) of 6.857, a mean expected heterozygosity (He) of 0.496 3, a mean observed heterozygosity (Ho) of 0.503 7, a mean genetic diversity index of Nei՚s of 0.494 9, and a mean Shannon information index of 0.995 8. These results suggest that the C. praecox population in Yanling exhibits a rich genetic diversity. Additionally, the population structure and the UPDM clustering were examined. In the GLM model, a total of fifteen marker loci exhibited significant (P < 0.05) association with eight phenotypic traits, with the explained phenotypic variation ranging from 14.90% to 36.03%. The construction of fingerprints for C. praecox varieties (lines) was accomplished by utilizing eleven primer pairs with the highest polymorphic information content, resulting in the analysis of 175 SSR markers. The present study offers a thorough examination of the genetic diversity and SSR molecular markers of C. praecox in Yanling, and establishes a fundamental germplasm repository of C. praecox, thereby furnishing theoretical underpinnings for the selection and cultivation of novel and superior C. praecox varieties, varietal identification, and resource preservation and exploitation.
Subject(s)
Bayes Theorem , Biomarkers , Phenotype , Cluster Analysis , Genetic VariationABSTRACT
Platycodonis Radix is the dry root of Platycodon grandiflorum of Campanulaceae, which has a variety of pharmacological effects and is a commonly used bulk Chinese medicine. In this study, the chloroplast genome sequences of six P. grandiflorum from different producing areas has been sequenced with Illumina HiSeq X Ten platform. The specific DNA barcodes were screened, and the germplasm resources and genetic diversity were analyzed according to the specific barcodes. The total length of the chloroplast genome of 6 P. grandiflorum samples was 172 260-172 275 bp, and all chloroplast genomes showed a typical circular tetrad structure and encoded 141 genes. The comparative genomics analysis and results of amplification efficiency demonstrated that trnG-UCC and ndhG_ndhF were the potential specific DNA barcodes for identification the germplasm resources of P. grandiflorum. A total of 305 P. grandiflorum samples were collected from 15 production areas in 9 provinces, for which the fragments of trnG-UCC and ndhG_ndhF were amplificated and the sequences were analyzed. The results showed that trnG-UCC and ndhG_ndhF have 5 and 11 mutation sites, respectively, and 5 and 7 haplotypes were identified, respectively. The combined analysis of the two sequences formed 13 haplotypes (named Hap1-Hap13), and Hap4 is the main genotype, followed by Hap1. The unique haplotypes possessed by the three producing areas can be used as DNA molecular tags in this area to distinguish from the germplasm resources of P. grandiflorum from other areas. The haplotype diversity, nucleotide diversity and genetic distance were 0.94, 4.79×10-3 and 0.000 0-0.020 3, respectively, suggesting that the genetic diversity was abundant and intraspecific kinship was relatively close. This study laid a foundation for the identification of P. grandiflorum, the protection and utilization of germplasm resources, and molecular breeding.
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ObjectiveTo study the genetic diversity and genetic relationship of Pinellia ternata germplasm resources and provide the basis for germplasm identification， variety breeding， and resource conservation. MethodIn this study， 27 P. ternata were used as experimental materials to determine seven phenotypic characters， such as plant height， leaf length， and leaf width. Simple sequence repeats （SSR） primers were designed based on P. ternata transcriptome data， and polymerase chain reaction （PCR） amplification was performed on 27 P. ternata samples. The genetic diversity of P. ternata germplasm was analyzed by POPGENE32， PowerMarker V3.25， and NTSYS-PC 2.10e software. ResultA total of 10 pairs of highly polymorphic primers （PIC>0.5） and four pairs of moderately polymorphic primers （0.25<PIC<0.5） were selected. The average number of alleles detected was 3.928 6， and the average Nei's diversity index （H） and Shannon's index （I） were 0.557 8 and 1.002 9， respectively， indicating a high level of genetic diversity. Cluster analysis divided the Pinellia ternata into seven categories， and P. ternata in the same province were in the same categories. The SSR molecular ID cards of 27 P. ternata germplasm were constructed with 14 pairs of primers， and the rapid identification of P. ternata in each region was realized. ConclusionThe results of this study can lay a foundation for the genetic diversity and population structure of P. ternata and provide a scientific basis for the identification of P. ternata germplasm resources， map construction， and molecular-assisted breeding.
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@#Culicoides oxystoma Kieffer is a vector of viruses, filarial nematodes and protozoa of the genus Leishmania transmitted to humans and other animals. Understanding genetic diversity, genetic structure and genetic relationships among geographically widespread populations will provide important information related to disease epidemiology. In this study, genetic diversity, genetic structure and genetic relationships between Thai C. oxystoma and those reported from other countries were inferred based on mitochondrial cytochrome oxidase I (COI) and nuclear internal transcribed spacer 1 (ITS-1) sequences. A high level of genetic diversity was found in C. oxystoma from Thailand. The maximum K2P intraspecific genetic divergence for COI gene and ITS-1 sequences were 4.29% and 6.55%, respectively. Despite high genetic diversity, no significant genetic differentiation was found within the 13 Thai populations. This could be a result of unspecialized habitat requirement of the larval habitat, abundance and continuous distribution of host blood sources, potential for long distance movement with host via trading. Mitochondrial genealogy analysis of the global population of C. oxystoma revealed three (A, B and C) genetically divergent lineages. Specimens from Thailand were included in the main lineage (A) with those from all other countries except those from Senegal that formed lineage B and those of Lineage C that was exclusively found in Bangladesh. The nuclear (ITS-1) genetic markers genealogy indicated that Thai C. oxystoma belong to the same genetic lineage with those from East, South and Southeast Asia which presumably the true C. oxystoma.
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By reviewing the research history on quality comparison between wild and cultivated Chinese crude drugs， this paper systematically combed the relevant research reports since the 1950s， and summarized and analyzed the results of existing comparative studies， and found that the existing comparative research on the quality of wild and cultivated Chinese crude drugs were mainly focused on several aspects， including characteristics， microstructures， chemical compositions， pharmacodynamic effects， and genetic diversity. Among these， comparative studies of chemical compositions have been the dominant approach， with a particular emphasis on comparing the contents of index components. However， research on pharmacodynamic effects remained relatively limited. Due to various factors such as sample quantity， sample origin， growth period and cultivation methods， the differences in quality between wild and cultivated Chinese crude drugs vary significantly. In general， most wild Chinese crude drugs exhibited higher quality than cultivated products， with significant differences in their characteristics. The contents and proportions of some chemical components underwent noticeable changes， particularly with a marked increase in the proportion of primary metabolites after cultivation. The quality of cultivated Chinese crude drugs is closely related to the cultivation practices employed. Chinese crude drugs produced through wild nurturing， simulated wild planting， ecological cultivation， and other similar methods demonstrate quality levels comparable to those of wild Chinese crude drugs. Based on the analysis results， it is recommended to explicitly specify the cultivation practices and cultivation period of cultivated Chinese crude drugs in comparative studies of the quality between wild and cultivated Chinese crude drugs. Multiple technical approaches， including characteristics， microscopy， non-targeted metabolomics combined with quantitative analysis of differential components， and bioefficacy evaluation， should be employed to comprehensively assess the quality disparities between wild and cultivated Chinese crude drugs. Moreover， research efforts should be intensified to investigate the changes in pharmacodynamic effects resulting from differences in plant cell wall composition， primary metabolites， and secondary metabolites， in order to guide the production of high-quality Chinese crude drugs.
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Abstract Several species of Cichla successfully colonized lakes and reservoirs of Brazil, since the 1960s, causing serious damage to local wildlife. In this study, 135 peacock bass were collected in a reservoir complex in order to identify if they represented a single dominant species or multiple ones, as several Cichla species have been reported in the basin. Specimens were identified by color pattern, morphometric and meristic data, and using mitochondrial markers COI, 16S rDNA and Control Region (CR). Overlapping morphological data and similar coloration patterns prevented their identification using the taxonomic keys to species identification available in the literature. However, Bayesian and maximum likelihood from sequencing data demonstrated the occurrence of a single species, Cichla kelberi. A single haplotype was observed for the 16S and CR, while three were detected for COI, with a dominant haplotype present in 98.5% of the samples. The extreme low diversity of the transplanted C. kelberi evidenced a limited number of founding maternal lineages. The success of this colonization seems to rely mainly on abiotic factors, such as increased water transparency of lentic environments that favor visual predators that along with the absence of predators, have made C. kelberi a successful invader of these reservoirs.
Resumo Muitas espécies de Cichla colonizaram com sucesso lagos e reservatórios do Brasil desde os anos 1960, causando graves prejuízos à vida selvagem nesses locais. Neste estudo, 135 tucunarés foram coletados em um complexo de reservatórios a fim de identificar se representavam uma espécie dominante ou múltiplas espécies, uma vez que diversas espécies de Cichla foram registradas na bacia. Os espécimes foram identificados com base na coloração, dados morfométricos e merísticos, e por marcadores mitocondriais COI, 16S rDNA e Região Controle (RC). A sobreposição dos dados morfométricos e o padrão similar de coloração impediram a identificação utilizando as chaves de identificação disponíveis na literatura. Entretanto, as análises bayesiana e de máxima verossimilhança de dados moleculares demonstraram a ocorrência de uma única espécie, Cichla kelberi. Um único haplótipo foi observado para o 16S e RC, enquanto três foram detectados para o COI, com um haplótipo dominante presente em 98,5% das amostras. A baixa diversidade nos exemplares introduzidos de C. kelberi evidenciou um número limitado de linhagens maternas fundadoras. O sucesso da invasão parece depender de fatores abióticos, como a maior transparência da água de ambientes lênticos que favorece predadores visuais que, atrelado à ausência de predadores, fez do C. kelberi um invasor bem-sucedido nesses reservatórios.
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Abstract Domestic donkey plays a key role as a draft animal in rural economy of Pakistan where its population is increasing every year. The complete mtDNA control region of forty randomly sampled donkeys was PCR- amplified and sequenced bi-directionally using specific primers. Distinct mtDNA haplotypes obtained in the current study (KY446001KY446011) were subjected to haplotype (h) and nucleotide diversity () measures using DnaS as well as to phylogenetic, Network, and AMOVA analyses. There were a total 27 polymorphic sites present within 11 unique mtDNA haplotypes from the studied 40 animals from different regions. Neighbor-joining network and median-joining network both illustrated the splitting of all these haplotypes into two well-defined Nubian and Somali lineages, confirming African maternal origin of Pakistani domestic donkey. Diversity parameters h (0.967± 0.037) and (0.02917± 0.00307) were found to reveal high levels of genetic diversity in Pakistani donkeys. AMOVA demonstrated only 1% of genetic differences between two mtDNA maternal lineages, pointing to lack of population substructure in Pakistani donkeys as is the case with worldwide domestic donkey population. Pakistani donkeys have African maternal origin and high levels of mtDNA diversity. High genetic diversity may be due to non-selective breeding and heteroplasmy. We herein provide the first report on mtDNA diversity of control region in Pakistani domestic donkey.
Resumo O burro doméstico possui um papel fundamental como animal de tração na economia rural do Paquistão, onde a população desse animal está aumentando a cada ano. A região de controle de mtDNA completa de 40 burros amostrados aleatoriamente foi ampliada por PCR e sequenciada bidirecionalmente por intermédio de primers específicos. Haplótipos distintos de mtDNA obtidos no estudo atual (KY446001 KY446011) foram submetidos a medidas de haplótipo (h) e diversidade de nucleotídeos () por meio de DnaS, bem como análises filogenéticas, de rede e AMOVA. Havia um total de 27 sítios polimórficos presentes em 11 haplótipos de mtDNA exclusivos dos 40 animais estudados de diferentes regiões. A rede de união de vizinhos e a rede de união mediana ilustram a divisão de todos esses haplótipos em duas linhagens núbias e somalis bem definidas, confirmando a origem materna africana do burro doméstico do Paquistão. Os parâmetros de diversidade h (0,967 ± 0,037) e (0,02917 ± 0,00307) revelaram altos níveis de diversidade genética em burros paquistaneses. AMOVA demonstrou apenas 1% de diferenças genéticas entre as duas linhagens maternas de mtDNA, apontando a falta de subestrutura populacional em burros paquistaneses, como é o caso da população mundial de burros domésticos. Os burros paquistaneses têm origem materna africana e altos níveis de diversidade de mtDNA. A alta diversidade genética pode ser por causa da reprodução não seletiva e de heteroplasmia. Aqui, fornecemos o primeiro relatório sobre a diversidade do mtDNA da região de controle em burros domésticos do Paquistão.
ABSTRACT
Domestic donkey plays a key role as a draft animal in rural economy of Pakistan where its population is increasing every year. The complete mtDNA control region of forty randomly sampled donkeys was PCR- amplified and sequenced bi-directionally using specific primers. Distinct mtDNA haplotypes obtained in the current study (KY446001−KY446011) were subjected to haplotype (h) and nucleotide diversity (π) measures using DnaS as well as to phylogenetic, Network, and AMOVA analyses. There were a total 27 polymorphic sites present within 11 unique mtDNA haplotypes from the studied 40 animals from different regions. Neighbor-joining network and median-joining network both illustrated the splitting of all these haplotypes into two well-defined Nubian and Somali lineages, confirming African maternal origin of Pakistani domestic donkey. Diversity parameters h (0.967± 0.037) and π (0.02917± 0.00307) were found to reveal high levels of genetic diversity in Pakistani donkeys. AMOVA demonstrated only 1% of genetic differences between two mtDNA maternal lineages, pointing to lack of population substructure in Pakistani donkeys as is the case with worldwide domestic donkey population. Pakistani donkeys have African maternal origin and high levels of mtDNA diversity. High genetic diversity may be due to non-selective breeding and heteroplasmy. We herein provide the first report on mtDNA diversity of control region in Pakistani domestic donkey.
O burro doméstico possui um papel fundamental como animal de tração na economia rural do Paquistão, onde a população desse animal está aumentando a cada ano. A região de controle de mtDNA completa de 40 burros amostrados aleatoriamente foi ampliada por PCR e sequenciada bidirecionalmente por intermédio de primers específicos. Haplótipos distintos de mtDNA obtidos no estudo atual (KY446001 − KY446011) foram submetidos a medidas de haplótipo (h) e diversidade de nucleotídeos (π) por meio de DnaS, bem como análises filogenéticas, de rede e AMOVA. Havia um total de 27 sítios polimórficos presentes em 11 haplótipos de mtDNA exclusivos dos 40 animais estudados de diferentes regiões. A rede de união de vizinhos e a rede de união mediana ilustram a divisão de todos esses haplótipos em duas linhagens núbias e somalis bem definidas, confirmando a origem materna africana do burro doméstico do Paquistão. Os parâmetros de diversidade h (0,967 ± 0,037) e π (0,02917 ± 0,00307) revelaram altos níveis de diversidade genética em burros paquistaneses. AMOVA demonstrou apenas 1% de diferenças genéticas entre as duas linhagens maternas de mtDNA, apontando a falta de subestrutura populacional em burros paquistaneses, como é o caso da população mundial de burros domésticos. Os burros paquistaneses têm origem materna africana e altos níveis de diversidade de mtDNA. A alta diversidade genética pode ser por causa da reprodução não seletiva e de heteroplasmia. Aqui, fornecemos o primeiro relatório sobre a diversidade do mtDNA da região de controle em burros domésticos do Paquistão
Subject(s)
Animals , Pakistan , Genetic Variation , DNA, Mitochondrial , EquidaeABSTRACT
Abstract Several species of Cichla successfully colonized lakes and reservoirs of Brazil, since the 1960's, causing serious damage to local wildlife. In this study, 135 peacock bass were collected in a reservoir complex in order to identify if they represented a single dominant species or multiple ones, as several Cichla species have been reported in the basin. Specimens were identified by color pattern, morphometric and meristic data, and using mitochondrial markers COI, 16S rDNA and Control Region (CR). Overlapping morphological data and similar coloration patterns prevented their identification using the taxonomic keys to species identification available in the literature. However, Bayesian and maximum likelihood from sequencing data demonstrated the occurrence of a single species, Cichla kelberi. A single haplotype was observed for the 16S and CR, while three were detected for COI, with a dominant haplotype present in 98.5% of the samples. The extreme low diversity of the transplanted C. kelberi evidenced a limited number of founding maternal lineages. The success of this colonization seems to rely mainly on abiotic factors, such as increased water transparency of lentic environments that favor visual predators that along with the absence of predators, have made C. kelberi a successful invader of these reservoirs.
Resumo Muitas espécies de Cichla colonizaram com sucesso lagos e reservatórios do Brasil desde os anos 1960, causando graves prejuízos à vida selvagem nesses locais. Neste estudo, 135 tucunarés foram coletados em um complexo de reservatórios a fim de identificar se representavam uma espécie dominante ou múltiplas espécies, uma vez que diversas espécies de Cichla foram registradas na bacia. Os espécimes foram identificados com base na coloração, dados morfométricos e merísticos, e por marcadores mitocondriais COI, 16S rDNA e Região Controle (RC). A sobreposição dos dados morfométricos e o padrão similar de coloração impediram a identificação utilizando as chaves de identificação disponíveis na literatura. Entretanto, as análises bayesiana e de máxima verossimilhança de dados moleculares demonstraram a ocorrência de uma única espécie, Cichla kelberi. Um único haplótipo foi observado para o 16S e RC, enquanto três foram detectados para o COI, com um haplótipo dominante presente em 98,5% das amostras. A baixa diversidade nos exemplares introduzidos de C. kelberi evidenciou um número limitado de linhagens maternas fundadoras. O sucesso da invasão parece depender de fatores abióticos, como a maior transparência da água de ambientes lênticos que favorece predadores visuais que, atrelado à ausência de predadores, fez do C. kelberi um invasor bem-sucedido nesses reservatórios.
Subject(s)
Animals , Cichlids/genetics , Phylogeny , Genetic Variation/genetics , Haplotypes/genetics , Lakes , Bayes TheoremABSTRACT
So far, the monoclonal hypothesis of tumor occurrence and development cannot be justified. The genetic diversity selection hypothesis for the occurrence and development of lung cancer links Mendelian genetics with Darwin's theory of evolution, suggesting that the genetic diversity of tumor cell populations with polyclonal origins-monoclonal selection-subclonal expansion is the result of selection pressure. Normal cells acquire mutations in oncogenic driver genes and have a selective advantage over other cells, becoming tumor initiating cells; In the interaction with the tumor microenvironment (TME), the vast majority of initiating cells are recognized and killed by the human immune system. If immune escape occurs, the incidence of malignant tumors will greatly increase, and subclonal expansion, intratumour heterogeneity, etc. will occur. This article proposed the hypothesis of genetic diversity selection and analyzed its clinical significance. .
Subject(s)
Humans , Lung Neoplasms/genetics , Clinical Relevance , Evolution, Molecular , Mutation , Tumor MicroenvironmentABSTRACT
Aims: Owing to its export value in flower trade elsewhere in the world, Rose is the key commercial flower crop and the area under rose cultivation is ever increasing and the end-users always prefer new color variations. Hence, evolving new cultivars with novel color characteristics is the need of the hour, for which understanding genetic variation in the available cultivars is very much needed. Study Design: This investigation was conducted to analyze the genetic diversity of 11 elite and commonly cultivated rose accessions in South India by using randomly amplified polymorphic DNA (RAPD) markers.Place and Duration of Study: Department of Floriculture and Landscape Architecture, Horticultural College and Research Institute, TNAU, Coimbatore.Methodology: A total of 10 RAPD primers were employed, which was sufficient to distinguish the investigated rose cultivars.Results: Among the 44 PCR products produced by these markers, 39 (88.64%) were found to be polymorphic bands. The number of amplified products per RAPD primer varied from 3 to 8 with a mean of 4.4 bands per primer. The Un weighted paired group of arithmetic means (UPGMA) dendrogram distinguished the rose accessions into two major clusters suggesting that the accessions were different from each other. The genetic similarity coefficients were determined with this RAPD data, and they were ranged from 0.59 to 0.89.Conclusion: Molecular profiling data of this study have contributed to characterize and catalogue the rose germplasm data, which will be useful to identify the diverse rose lines for further breeding program that have the potential to improve the color variations.
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Introducción: El pargo mancha es un pez marino de alto consumo e interés comercial en Costa Rica que está sometido a una fuerte presión pesquera, la cual puede afectar la diversidad genética y generar problemas por depresión endogámica. Objetivo: Evaluar el estado genético de la población de Lutjanus guttatus mediante el uso microsatélites. Métodos: Se recolectaron muestras entre el 2018 y 2019 y se estudiaron 44 individuos de cada una de las localidades del Golfo de Nicoya y Golfo Dulce. Se realizó la extracción de ADN y la amplificación de diez loci con microsatélites mediante PCR, para la determinación del genotipo, análisis de diversidad genética y estructura poblacional. Resultados: Los parámetros de diversidad indican un elevado polimorfismo asociado con un alto número de alelos obtenidos por locus, pero con bajos niveles de heterocigosidad observada en comparación con la esperada (Ho= 0.774 y 0.800 y He= 0.948 y 0.954 para Golfo de Nicoya y Golfo Dulce, respectivamente). No hay evidencia suficiente para decir que las dos poblaciones son distintas (FST= 0.00264, P > 0.05). La desviación del Equilibrio de Hardy-Weinberg indica la posible mezcla de organismos de origen distinto a los del medio silvestre. Conclusiones: L. guttatus tiene niveles altos de diversidad genética, no hay evidencia de diferenciación en subpoblaciones genéticas, lo que en manejo pesquerías se considera una sola población panmíctica. La posible mezcla de individuos de origen distinto al silvestre sugiere la presencia de organismos de un programa de repoblación o de cultivos comerciales en la región. El uso de marcadores genéticos se recomienda para el monitoreo, además, en programas de repoblación y evaluar su efecto.
Introduction: The spotted snapper is a high-consumption and commercially important marine fish in Costa Rica, subjected to heavy fishing pressures, which can affect genetic diversity and generate problems due to inbreeding depression. Objective: To evaluate the genetic status of the population of Lutjanus guttatus using microsatellites. Methods: Samples were collected between 2018 and 2019, and 44 individuals from each of the localities of the Gulf of Nicoya and the Gulf of Dulce were studied. DNA extraction and amplification of ten loci with microsatellites using PCR were performed, followed by genotyping, analysis of genetic diversity, and population structure. Results: Diversity parameters indicate a high polymorphism associated with a high number of alleles obtained per locus, but with low levels of observed heterozygosity compared to expected (Ho= 0.774 and 0.800, and He= 0.948 and 0.954 for the Gulf of Nicoya and Gulf of Dulce, respectively). There is not enough evidence to say that the two populations are distinct (FST= 0.00264, P > 0.05). Deviation from Hardy-Weinberg equilibrium was recorded, indicating possible mixing of organisms of different origin from the wild environment. Conclusions: L. guttatus presents high levels of genetic diversity, without evidence of differentiation in genetic subpopulations. For fisheries management purposes, they would be considered a single panmictic population. The possible mixing with wild individuals suggests the presence of organisms derived from a restocking or commercial cultivation program carried out in the region. The use of genetic markers is recommended to maintain monitoring, follow up on restocking programs and evaluate their effect.
Subject(s)
Animals , Animals, Inbred Strains/growth & development , Fishes/growth & development , Costa Rica , Genetic FitnessABSTRACT
ABSTRACT The human X-chromosome non-coding markers, such as short tandem repeats (STRs), single nucleotide polymorphisms (SNPs), insertion-deletions (INDELs) and Alu insertions, are useful for revealing relationships among populations and for the identification of individuals. In the last decades, a number of studies have been performed to determine the genetic structure of Latin American populations by using X-chromosome markers. These studies provided useful information regarding the genetic composition of these populations and their relationship with Native American, Asian and European populations. One of the most interesting findings achieved by X-chromosome studies is the bias in the sex ratio of individuals that gave rise to the current Latin American populations, as it was previously observed through the analysis of uniparental markers, and which is undoubtedly evidenced in the differential inheritance of X-chromosome in comparison to autosomes. Besides, the genetic drift process that affected Native American populations is more pronounced in X-chromosome markers than in autosomes. The present review summarizes our current knowledge concerning X-chromosome non-coding polymorphisms studied in Latin American populations.
RESUMEN Los marcadores no codificantes del cromosoma X humano, como las repeticiones cortas en tándem (STR), los polimorfismos de un solo nucleótido (SNP), las inserciones-deleciones (INDEL) y las inserciones Alu, son útiles para revelar la relación existente entre poblaciones, y también para la identificación de personas. En las últimas décadas, se han realizado una serie de estudios para determinar la estructura genética de las poblaciones latinoamericanas, utilizando marcadores de cromosoma X. Estos estudios proporcionaron información útil sobre la composición genética de estas poblaciones y su relación con las poblaciones nativas americanas, asiáticas y europeas. Uno de los hallazgos más interesantes logrados en estos estudios es el sesgo en la proporción de sexos de los individuos que originaron las poblaciones latinoamericanas actuales, tal como se observó previamente a través del análisis de marcadores uniparentales, y que queda evidenciado por la herencia diferencial del cromosoma X en comparación con los autosomas. Además, el proceso de deriva genética que afectó a las poblaciones nativas americanas actuó de manera más pronunciada en los marcadores del cromosoma X que en los autosomas. La presente revisión resume nuestro conocimiento actual sobre los polimorfismos no codificantes del cromosoma X estudiados en poblaciones latinoamericanas.
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Leguminous crops are important components of Indian horticulture, because of being an important source of nutrition in predominantly vegetarian diet. In order to investigate the genetic diversity present in the underutilized legume sword bean genotypes of North-Eastern hill region of India 20 native sword bean (Canavalia gladiata Jacq.) genotypes were collected from different agroclimatic region of North-Eastern hill region of India. The morphological characters were observed in days to seed germination i.e. (3-6 days), pod length (16.45-32.87 cm), pod width (2.28-4.33 cm), pod weight (17.92-31.93 g), pod girth (7.75-12.64 mm) and seed weight (0.75-2.35 g). Performance of CHF SB-1, CHF SB-18, CHF SB-11, CHF SB-19, CHF SB-15, CHF SB-4 and CHF SB-10 genotypes under test conditions was superior and suggested that breeders might exploit the genome of these genotypes in current pulse crop improvement programmes.
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Aim: To determine the extent of genetic divergence (D2 statistics) present in sesame genotypes for different traits.Study Design: The experiment was conducted in Augmented Block Design (ABD).Place and Duration of Study: The experiment was laid out in kharif 2020 at BSP (Groundnut) Research Farm, JNKVV, Jabalpur (M.P.).Methodology: Total 160 sesame genotypes and 5 checks viz., RT346, PBTil2, GT10, TMV-7 and VRI-1 were used for diversity analysis for twelve quantitative traits for selection of diverse parents. The 165 genotypes were grouped into eight clusters based on the Mahalanobis D2 values following Tocher’s methods.Results: Maximum inter cluster distance (63.235) was exhibited between cluster III and cluster VIII and intra cluster distance (28.509) was in cluster VII. The lowest inter cluster divergence (15.881) was noticed between cluster III and V indicating that the genotypes included in them were closely related. Relative contribution of different characters to total divergence was assessed through comparison of actual D2 values for individual characters over all possible combinations. Cluster III had highest mean value for plant height (126.609), days to flower initiation (34.308), days to 50% flowering (37.462), and oil content (44.715). Cluster VII reported to be days to maturity (91.750), number of primary branches per plant (3.833) and number of secondary branches per plant (2.625) and in cluster V had capsule length (2.651). cluster VI reported for number of capsules per plant (59.484) and seed yield per plant (9.168), number of seeds per capsule (58.533) and thousand seed weight (2.848). It was revealed that number of primary branches per plant (11.32%) followed by oil content (11.06%) and number of capsules per plant (11.05%) contributed maximum to total divergence and minimum contributed by days to flower initiation (3.49%).Conclusion: So, it is expected that crosses between genotypes of cluster III with genotypes of cluster VIII will give rise to high yielding sergeants as high inter cluster distance and those clusters has recorded good mean values can be preferred in selecting germplasm lines for respective traits.
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Plantation forestry plays a vital role in combating climate change and promoting sustainable development by providing renewable resources, sequestering carbon, and supporting economic growth. This review examines the contributions of plantation forestry to climate change mitigation, including carbon sequestration and substitution effects, as well as the challenges and opportunities for sustainable development. We explore key aspects such as genetic diversity, pest and disease management, soil conservation, social dimensions, and emerging technologies. By assessing the current state of plantation forestry and identifying future directions, this review highlights the importance of plantation forestry in addressing global environmental challenges while fostering sustainable development.
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The study aimed to assess the genetic diversity of twenty-two elite capsicum (Capsicum annuum L.) germplasm lines across nine quanlitative traits. The research was conducted at the research farm of ICAR-Indian Institute Horticultural Research, Hessaraghatta, Bengaluru, Karnataka, during 2014-2015 period. The experimental design employed was a randomized complete block design with three replications. The quality parameters under study included fruit length (cm), fruit width (cm), pericarp thickness (mm), placenta length (cm), placenta width (cm), total soluble solids (°Brix), fruit to seed ratio, seed number and total capsaicinoids (Scoville Heat Units). Based on the performance of the study, a cluster analysis of twenty-two chilli germplasm lines were grouped into three clusters. Cluster I had a maximum of sixteen genotypes and clusters III had a minimum of one genotype. The highest inter–cluster distance (60.521) was observed in between clusters I and II and the lowest (17.964) in between clusters I and III. Among the traits examined, fruit length and total capsaicinoids contributed the most to the genetic divergence. Considering group cluster analysis, mean performance and variability, it is recommended to explore inter genotypic crosses between cluster I and cluster II, as well as cluster I and cluster III, for future hybridization programmes in chilli crop improvement.
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Gall midge and brown spot of rice are a fungal disease that affect both seedlings and mature plants. To investigate resistance of rice to this disease twenty nine rice cultures were evaluated under irrigated ecosystem at Regional Agricultural Research Station, Jagtial during kharif, 2020. High heritable estimates were observed for all the 8 traits studied. High estimates of heritability was recorded for important yield attributes viz., days to 50% flowering (91.4, 11.24), plant height (77.8, 9.53), 1000-grain weight (98.4, 54.5), number of grains per panicle (78.3, 40.18) and grain yield (98.7, 33.87) indicated that these traits were under the control of additive genes. Significantly positive correlations were recorded with plant height, Number of productive tillers per plant and 1000 seed weight at both genotypic and phenotypic levels, the results clearly indicated that long duration genotypes with more height and bolder grains contribute for more grain yield. Among the eight traits studied, 1000 grain weight contributed highest (67.8%) towards total divergence followed by days to 50% flowering (11.5%), number of grains per panicle (6.06) and Galls midge incidence percentage (6.06%). Genotypes, JGL 38957 of cluster II, JGL 38950 of cluster III, JGL 38935 of cluster IV and JGL 38921 of Cluster VII could be the best source to develop brown spot, gall midge resistance and high yielding rice varieties.