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1.
Article in Chinese | WPRIM | ID: wpr-697446

ABSTRACT

Objective: To observe the effects of He-Ne laser on the expression of extracellular matrix and its regulatory factors in degenerated temporomandibular joint(TMJ) of rabbits. Methods: 40 New Zealand adult white rabbits were randomly divided into normal group,sham model group,TMJOA model group and laser treatment group(n = 10),the rats in treatment group were treated by He-Ne laser irradination at acupoints. The rats in each group were divided into 1 d and 11 d groups(n = 5). The animals were respectively killed 1 d and 11 d after operation and HE staining was used to observe the histomorpholy. The protein expression of extracellular matrix and its regulatory factors were examined by Western blot. Results: After He-Ne laser treatment,the fiber layer of condylar cartilage was slightly loose,part of the fiber was newly produced. The level of Col-2,PRG-4,TIMP-1,BMP-2 was up-regulated and the of MMP-13 was down-regulated in the 11 d treatment group. Conclusion: He-Ne laser irradiation on acupoints may up-regulate the expression of extracellular matrix (Col-2 and PRG-4) and its regulatory factors (TIMP-1 and BMP-2),down-regulate the expression of MMP-13.

2.
International Eye Science ; (12): 1371-1373, 2017.
Article in Chinese | WPRIM | ID: wpr-641124

ABSTRACT

AIM: To evaluate the effect of He-Ne laser combined with TDP irradiation in the treatment of herpes zoster ophthalmicus.METHODS: Totally 22 patients (22 eyes) with herpes zoster ophthalmicus were treated by He-Ne laser irradiation combined with TDP (Group A).Another 22 patients (22 eyes) with herpes zoster ophthalmicus (Group B) were treated by medication.The duration of the disease, the incidence of complications, and the time of pain relief were compared between the two groups.RESULTS: The cure rate of Group A was 91%, that of Group B was 73%, there was no significant difference between the two groups (x2=1.375, P>0.05).The average cure time of Group A was 8±2.22d, Group B was 12±1.88d, the difference between the two groups was statistically significant (t=5.22, P<0.05).The complication rate of Group A was 18%, Group B was 59%, the difference between the two groups was statistically significant (x2=6.86, P<0.05).The average pain relief time of Group A was 5±1.23d, Group B was 10±1.34d, the difference between the two groups was statistically significant (t=11.17, P<0.05).CONCLUSION: He-Ne laser combined with TDP irradiation can shorten the treatment time of herpes zoster ophthalmicus, shorten the course of disease, reduce the incidence of complications, relieve pain, with no significant effect on the cure rate of herpes zoster ophthalmicus.

3.
Journal of Medical Postgraduates ; (12): 741-744, 2016.
Article in Chinese | WPRIM | ID: wpr-493430

ABSTRACT

Objective The medical code of biology-psychology-societyplays a vital role in modern treatment .The arti-cle aimed to investigate the clinical value of He-Ne laser combined with psychological intervention in the treatment of acute herpes and zoster. Methods Retrospective analysis was made on the clinical data of 81 patients with acute herpes and zoster in our hospital from Jan 2015 to Jan 2016 .The patients were divided into 3 groups randomly .Patients in group A were treated with antiviral and neu-rotrophic medication , psychological intervention and analgesic drugs . Patients in group B were treated with antiviral and neurotrophic medi-cation, psychological intervention and He-Ne laser.Patients in group C were treated with antiviral and neurotrophic medication , psychologi-cal intervention, analgesic drugs and He-Ne laser.Visual analogue scale ( VAS) was evaluated before treatment in hospital on the 3rd day, the 5th day of treatment, and telephone follow -up after 4 weeks of treatment.VAS and average length of stay were analyzed . Results No difference was found as to VAS before treatment in hospital among three groups (8.1 ±1.1, 8.1 ±0.9 vs 7.9 ±1.0, P<0.05).VAS was decreased in all groups after nursing propaganda , psychological intervention and placebo (P<0.05).Compared with the VAS before treatment, VAS in three groups decreased significantly on the 3rd day and the 5th day of treatment.From the re-sults on the 5th day and telephone follow -up after 4 weeks of treatment , VAS was still at a low level in group A and group B , on the contrary, VAS was decreased continuously in group C . Conclusion Combined treatment of antiviral , neurotrophic, analgesic drugs and He-Ne laser can relieve pain , decrease VAS and shorten the average length of stay for patients with acute herpes and zoster .

4.
Article in Chinese | WPRIM | ID: wpr-447587

ABSTRACT

Objective To compare healing effects of surgical incisions using 635 /808 nm dualwavelength semiconductor laser and He-Ne laser irradiation.Methods 168 cases of non-malignant tumor surgery patients were randomly divided into 2 groups:test group including 83 cases which were treated on the surgical incisions by laser irradiation of the semiconductor illumination with low-intensity power; positive control group with 85 cases treated with He-Ne laser.Observation was carried out on incision healing by the clinic manifestations including redness,heat,swelling,pain,exudation,wound open,adverse event and the incision length offset.Results There was no significant difference between experimental group and the control group on incision healing (P>0.05).Conclusions The treatment efficacy of the dual-wavelength semiconductor laser on surgical incisions healing is similar with that of the He-Ne laser.

5.
Article in Chinese | WPRIM | ID: wpr-434352

ABSTRACT

Objective To investigate the effects of He-Ne laser on the treatment of pericoronitis.Methods 75 clinical patients with pericoronitis were randomly divided into the experimental group and control group.Local conventional therapy was given to the two groups.The experimental group was given routine treatment with He-Ne laser local exposure for 15 minutes every day,while the control group took no other treatment.3 days and 7 days later,pain and inflammation degree were recorded by three-degree pain scoring method.Total treatment effects were evaluated under a comprehensive clinical treatment standard.Results After 3 days' treatment,78.9% patients in the experimental group and 51.4% patients in control group felt their pain disappeared.After 7 days' treatment,97.4% patients in experimental group and 91.9% patients in control group felt their pain disappeared.Treatment comprehensive evaluation showed that there were statistical significance differences between two groups (P<0.05).Conclusion He-Ne laser treatment of pericoronitis has significant anti-inflammatory analgesic effect.

6.
Article in English | WPRIM | ID: wpr-629191

ABSTRACT

The effect of low power 0.95 mW He-Ne laser irradiation (λ = 632.8 nm) on the subpopulations of human blood parameters such as hemoglobin concentration (HGB), mean cellular volume of red blood cell (MCV), and mean cellular hemoglobin (MCH) were investigated by electronic sizing at the Wellness Centre of Universiti Sains Malaysia (USM). These parameters were correlated with human characteristics such as age, gender, ethnic, and blood types. The correlations were obtained by finding patterns in changes of blood parameters after radiation, non-parametric tests using SPSS version 11.5, centroid and peak positions, and flux variations. The analysis revealed significant changes according to human characteristics, for age (p = 0.067), gender (p = 0.044), ethnic (p = 0.094), and blood types (p = 0.099). This finding shows that the centroid and peak positions, flux peak and total flux, were highly correlated with human characteristics and can become a significant indicator for blood analysis. Furthermore, the encircled flux analysis demonstrated a good future prospect in blood research, thus leading the way as a vibrant diagnosis tool to clarify diseases associated with blood.

7.
International Eye Science ; (12): 1650-1653, 2010.
Article in Chinese | WPRIM | ID: wpr-641411

ABSTRACT

AIM: To investigate the influence of He-Ne laser on connective tissue growth factor (CTGF) expression and collagen formation of fibroblast in filtration site after trabeculectomy in rabbit, and to discuss the mechanism for preventing scar formation with He-Ne laserin vivo.METHODS: The upper nasal limbus area next to the upper rectus muscle in right eyes Received 10 minutes He-Ne laser irradiation (200mW/cm2) every day for three days, the left eyes served as control. Twenty-four hours after the last irradiation, both eyes of the rabbits were took trabeculectomy surgery. The expressions of CTGF in the filtration area were tested on the 7th, 14th and 28th day after surgery and collagen density was tested on the 14th and 28th day after surgery. Each of the time point had 7 rabbits. RESULTS: The expression of CTGF was lower than that of the control group's on the 7th and 14th day after trabeculectomy surgery (P=0.01, P=0.005). When examined on the 14th and 28th day, the collagen density of irradiation group were significantly lower than that of the control group's (P=0.013, P=0.01).CONCLUSION: Pretreating the filtration area with 200mW/cm2 He-Ne laser may be helpful in preventing scar formation after trabeculectomy in rabbit, possibly due to downregulation of the expression of CTGF and collagen synthesis in fibroblasts. He-Ne laser may be developed into a new scar preventing method in filtration surgery.

8.
Article in Chinese | WPRIM | ID: wpr-559788

ABSTRACT

Objective To seek an ideal treatment for tinnitus.Methods Use the pioneering of Laser Meridian Points Therapy(LMPT).The domestic He-Ne laser therapeutic instrument was used with optical fiber wave length 632.8nm,output power 2~30mW,therapeutic power quartz optical fiber at the end of fiber power 1~10mW,diameter of optical patch at the end of the optical fiber 0.2cm,optical patch area 0.0314cm~2,power density 31.85~318.47mW/cm~2.Use the optical fiber end of the instrument to contact and irradiate the following points:Yifeng(SJ 17),Yiming(Extra),Fengch'ih(GB20),Yingyou,T'ingKung(SI 19),T'inghui(GB 2),Erhmen(SJ 21),Paihui(Du 20),et al.Each point each time was irradiated for 5~15 minutes of laser dosage(energy density)9.555~286.623J/cm~2,once a day,7~14 days a course of treatment.Results 1808 cases(3037 ears)tinnitus(patients)of 1469(81.25%)cases 2686(81.22%)ears showed disapperance,313(17.31%)cases 577(17.45%)ears showed marked or some therapeutic effectiveness,26(1.44%)cases 44(1.33%)ears was ineffective.Follow-up survey 1~9 years of 1139 case 2043 ears found 936(82.18%)cases 1678(82.14%)ears had their tinnitus disappeared,188(16.51%)cases 340(16.64%)ears showed marked effectiveness or some effectiveness,15(1.32%)cases 25(1.22%)ears was ineffective.Conclusions LMPT for the treatment of tinnitus is the pioneering technique we have developed.Better than any other therapies at present and having good short-term effectiveness and stable and ideal long-term effectiveness,LMPT is worthwhile for further study and popularized clinical application.

9.
Article in Chinese | WPRIM | ID: wpr-565015

ABSTRACT

Objective To study the effect of He-Ne laser at lower power on proliferation and differentiation of embryo midbrain nerve cell.Methods The embryo midbrain nerve cell of rat embryo were randomly divided into one control group and three experimental groups.The cells in experimental groups were respectively irradiated at different time:3 min(A组),6 min(B组),10min(C组),each day.After ten days,we counted the concentration rate and determined the content of protein and the amount of superoxide dismutase(SOD)and malonyldialolehyde(MDA).Results The concentration rate of group A and of group B significantly increased than that of the control group(P

10.
Article in Chinese | WPRIM | ID: wpr-561813

ABSTRACT

Objective The a seek in ideal of iatrotechnique is treatment of Traumatic Cerebral Tinnitus(TCT).Methods Use the pioneering of Laser Meridian Points Therapy(LMPT).The domestic He-Ne laser therapeutic instrument was used with optical fiber wave length 632.8nm,output power 2~30mW,therapeutic power quartz optical fiber at the end of fiber power 1~10 mW,diameter of optical patch at the end of the optical fiber 0.2cm,optical patch area 0.0314 cm2,power density 31.85~318.47mW/cm2.Use the optical fiber end of the instrument to contact and irradiated the following points:Yifeng(SJ 17),Yiming(Extra),Fengch'ih(GB 20),Yingyou,T'ingkung(SI 19),T'inghui(GB 2),Erhmen(SJ 21),Paihui(Du 20),et al.Each point each time was irradiated for 5~15 minutes of laser dosage(energy density)9.555~286.623J/cm2,once a day,7~14 days a course of treatment.Results 262 cases(503 ears)TCT(patients)of 215 cases(82.06%)414 ears(82.31%)showed tinnitus disapperance,45 case(17.18%)86 ears(17.10%)showed marked or some therapeutic effectiveness,2 case(0.76%)3 ears(0.59%)was ineffective.Follow-up survey 1~9 years of 157 cases 284 ears,found 141 case(89.81%)255 ears(89.78%)had their tinnitus disappeared,15 case(9.55%)27 ears(9.51%)showed marked effectiveness or some effectiveness,1 cases(0.64%)2 ears(0.70%)was ineffective.Conclusions LMPT for the treatment of TCT is the pioneering technique we have developed.Better than any other therapies at present and having good short-term effectiveness and stable and ideal long-term effectiveness.LMPT is worthwhile for further study and popularized clinical application.

11.
Article in Chinese | WPRIM | ID: wpr-575295

ABSTRACT

Objective To evaluate the effect of low intensity He-Ne laser irradiation on the level of hydroxyproline in the Achilles tendons of rabbits after experimental tenotomy, and to determine the best irradiation cycle. Methods Twenty-two male New Zealand rabbits, aged 10-12 weeks, were randomly divided into control and experimental groups. The control group received no laser irradiation after tenotomy. The experimental animals were treated with a He-Ne laser (632.8 nm, 18.9 mW) daily for 14 days. The treated animals received 10 minutes, 20 minutes or 30 minutes of irradiation daily. At the 14th day or the 28th day after the operation, the rabbits were sacrificed and the level of hydroxyproline ( Hyp) in their Achilles tendons was measured. Results Hyp was significantly higher in all experimental animals than that in the controls. Hyp level in the 20 minutes group was significantly higher than in the 10 minutes group. Hyp level in the 20 minutes group at the 28th day were significantly higher than at the 14th day. Conclusions He-Ne laser irradiation facilitates the synthesis of collagen and enhances the healing of A-chilles tendons. The best irradiation time might be 20 minutes.

12.
Article in Chinese | WPRIM | ID: wpr-571928

ABSTRACT

Objective To explore the feasibility of He-Ne laser irradiation in promoting orthodontic tooth movement and periodontal remodeling through observing the effect of low power laser on the expression and distribution of matrix metalloproteinases- 9(MMP- 9) during orthodontic tooth movement in rabbit. Methods Thirty rabbits were divided into 1d,3d,5d,7d,14d and 21d groups, each group were given a coil spring connected to the maxillary upper first molar to the upper incisor and fixed bilaterally by a ligature wire and set force to 80g. He-Ne laser irradiation was performed on the right side of the orthodontic tooth, and the left side served as control. Radiation time ranged from 1 day to 5 days, once a day, 15min each time. All of the tissue sections were proceeded with MMP- 9 immunohistochemical staining. MMP- 9 average gray scale in periodontal tissue of the orthodontic teeth was analyzed by a Computer Image Analyzing System. Results The expression level of MMP- 9 increased earlier and lasted for a longer time in the compression and tension area on the side of irradiation by He-Ne laser as compared to those on the control side. There was significant difference between the irradiation side and the control side in terms of the MMP- 9 expression gray scale in the tension zones at 5 and 7 days post-operation, and in the pressure zones at 3,5,7 day post-operation. Conclusion During the movement of orthodontic teeth, local irradiation with He-Ne laser could increase the expression of MMP-9. It was suggested that the irradiation with low power laser accelerate blood vessel and bone remodeling.

13.
Article in Chinese | WPRIM | ID: wpr-571575

ABSTRACT

Objective To explore the effect of intra ve nous irradiation of low energy He-Ne laser on plasma endothelin(ET) levels in p atients with acute cerebral infarction (ACI). Methods Eighty-five patients with ACI were randomly divided into two groups: In group s I, the patients were treated with low energy He-Ne laser intravenous irradiat ion combined with conventional treatment (group ILIB);In group II, the patients were only received the conventional treatment (conventional control group). The levels of plasmal ET were measured using radioimmunoassay before and 10, 20 days after the treatment, simultaneously 39 healthy subjects were examined for ET le vels and served as the normal control group. Results Before treatment, the plasmal ET level of ACI was significantly higher than th at of normal control group ( P 0.05). ConclusionIt was suggested that intravenous irradiation therapy with low energy He-Ne laser could inhibit ET release and facilitate the recovery of ACI patients.

14.
Article in Chinese | WPRIM | ID: wpr-682272

ABSTRACT

Objective To determine the effect of low power He Ne laser irradiation on expression of myocardial VEGF in precordial region of wistar rat. Methods VEGF expression in myocardium was studied by immunohistochemical methods,RT PCR and computer image analysis.Sixteen rats were divided equally into control and irradiated groups.He Ne laser (632 8?nm)was applied with the dose of 60 5?J/cm 2 in the irradiated group. Results The stain of VEGF immunohistochemistry of myocardium in irradiated group was stronger than that of in control group.The optic density in irradiated group(0 246?0 015)was higher than that of control group(0 218?0 012,P

15.
Article in Chinese | WPRIM | ID: wpr-675539

ABSTRACT

Objective: To investigate the effects of He-Ne laser irradiation on orthodontic bone remodeling. Method: Thirt y rabbits were randomly divided into 6 groups with 5 in each group.Another 5 r abbits with no treatment were used as the unforced control. The animals were a nesthetized with 0.05 mol/L pentabarbetal (2 ml/kg). The maxillary first molar a nd the incisor were connected with coil spring ,fixed with ligature wire. Orthod ontic force of 7.84 N was applied on the teeth.The left side was served as contr ol and the right side received irradiation of He-Ne laser at 20 mW with the wav e length of 632.8 nm. Periodontal tissue samples of the experimental teeth were obtainned 1,3,5,7,14 and 21 days respectively after irradation. Tartrate-resist ant acid phosphatase (TRAP) in the tissues was studied with histochemistry. Results: The activity of TRAP in the irradiated tissues was higher than that in the unirradiated 3 and 7 days after treatment (P

16.
Article in Vietnamese | WPRIM | ID: wpr-2079

ABSTRACT

20 healthy male students from 20-25 years of age were studied on the changes of peripheral blood indexes after the exact Hegu laser He-Ne irradiation with intensity 130 mW/cm2 for 2 minutes/point. 10 (changed most clearly) of 20 these students were irradiated laser outside the Hegu (1cm far from the Hegu) as the control group. Before irradiating, after laser 10, 30 minutes, vein blood was taken to investigate the following indexes: RBC, WBC, HGB, HCT, MCV, MCH, MCHC, PLT, NEUT/1, LYMPH/1, MDX/1, RDW-SD, MPV, P-LCR. The results showed that PLT, WBC, LYMPH/1 reduced markly after 10, 30 minutes (p<0.05); there were no differences of the other (RBC, HGB, HCT, MCV, MCH, MCHC, NEUT/1, RDW-SD, PDW, P-LCR) between before and after laser irradiation. There was no differences between before and after outside-Hegu laser He-Ne irradiation; the time when the peripheral blood indexes changed more clearly is at the 30th minute after irradiating.


Subject(s)
Lasers , Hematology
17.
Article in Vietnamese | WPRIM | ID: wpr-2078

ABSTRACT

The purpose of this study was to investigate the effect of He-Ne Laser irradiation on the change of some biological indicies in the experimental infected mice after 1, 3, 5, 7, 10 and 15 days of irradiation. Over 240 male mice (at the same age, weight of from 180 to 200 mg) were subjected to study and divided into 4 groups: normal and non-infected animals (group1- control1), infected animals without any treatment (group 2- control 2), infected animals treated daily by Vancocin with dose of 400 mg per kg of bodyweight (group 3) and infected animals irradiated by He - Ne Laser everday with power density of 2mW/cm2 in 10 minutes (group 4). The control and experimental animals were sacrified at 1st,3nd, 5th, 7th,10th and 15th day which were time of post- irradiation in group 4. The hematological indices, immuoparameter and piece of skin flap were evaluated. The result of experimental study showed: + After one day of irradiation: in all infected groups (2, 3, 4), skin flaps were suppurative. There was not statistically significant difference of hematological indices and immunoparameters among groups. + After 3 days of irradiation, the hematological indicies and immunoparameters increased in grop 2, 3 and 4 in comparision with group 1. However, in group 4, the number of white blood cells was smaller and immunoparameters were higher than those in group 2. In addition, the process of wound healing (epitheliazation) in group 4. This proved that Hene laser irradiation started to have effectiveness at the 3rd day of post- irradiation. + After 5-10 days of irradiation, the hematological indices and immunoparameters returned to normal levels as group1 while these indices were still high in group 2. Furthermore, the skin flaps of group4 were completely wound- healed and gradually scarred over.This illustrated that there was an early regulation of biological indices the normalisation in irradiated mice. (Group 4). + Until the 15th day, the biological indices of group 2 were just normalised.


Subject(s)
Lasers , Hematologic Diseases , Animals
18.
Microbiology ; (12)1992.
Article in Chinese | WPRIM | ID: wpr-684988

ABSTRACT

The spores suspension of Streptomyces roseosporus D-38 irritated with 20mW He-Ne laser for 20 min were incubated on G1 medium plates containing 1. 9?g/mL of streptomycin. Ten percent of mutants increased the potency of daptomycin by streptomycin-resistance method, including the mutant LC-54, which could produce daptomycin 81. 2 mg/L, which was 39% higher than that of the beginning strain by flask fermentation.

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