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1.
Arq. bras. cardiol ; 118(4): 756-765, Apr. 2022. tab, graf
Article in Portuguese | LILACS-Express | LILACS | ID: biblio-1374346

ABSTRACT

Resumo Fundamento Demonstrou-se recentemente que a aplicação de ultrassom de alta energia com microbolhas, técnica conhecida como sonotrombólise, causa a dissolução de trombos intravasculares e aumenta a taxa de recanalização angiográfica no infarto agudo do miocárdio com supradesnivelamento do segmento ST (IAM-CSST). Objetivo Avaliar o efeito da sonotrombólise nos índices de motilidade e perfusão miocárdicas em pacientes com IAM-CSST, utilizando a ecocardiografia com perfusão miocárdica em tempo real (EPMTR). Método Uma centena de pacientes com IAM-CSST foram randomizados em dois grupos: Terapia (50 pacientes tratados com sonotrombólise e angioplastia coronária primária) e Controle (50 pacientes tratados com angioplastia coronária primária). Os pacientes realizaram EPMTR para analisar a fração de ejeção do ventrículo esquerdo (FEVE), o índice de escore de motilidade segmentar (IEMS) e o número de segmentos com defeito de perfusão miocárdica, 72 horas após o IAM-CSST e com 6 meses de acompanhamento. Foi considerado significativo p < 0,05. Resultados Pacientes tratados com sonotrombólise apresentaram FEVE mais alta que o grupo Controle em 72 horas (50 ± 10% vs. 44 ± 10%; p = 0,006), e essa melhora foi mantida em seis meses (53 ± 10% vs. 48 ± 12%; p = 0,008). O IEMS foi similar nos grupos Terapia e Controle em 72 horas (1,62 ± 0,39 vs. 1,75 ± 0,40; p = 0,09), mas tornou-se menor no grupo Terapia em 6 meses (1,46 ± 0,36 vs. 1,64 ± 0,44; p = 0,02). O número de segmentos com defeito de perfusão não foi diferente entre os grupos em 72 horas (5,92 ± 3,47 vs. 6,94 ± 3,39; p = 0,15), mas ficou menor no grupo Terapia em 6 meses (4,64 ± 3,31 vs. 6,57 ± 4,29; p = 0,01). Conclusão A sonotrombólise em pacientes com IAM-CSST resulta na melhora dos índices de motilidade e perfusão ventricular ao longo do tempo.


Abstract Background It has recently been demonstrated that the application of high-energy ultrasound and microbubbles, in a technique known as sonothrombolysis, dissolves intravascular thrombi and increases the angiographic recanalization rate in patients with ST-segment-elevation myocardial infarction (STEMI). Objective To evaluate the effects of sonothrombolysis on left ventricular wall motion and myocardial perfusion in patients with STEMI, using real-time myocardial perfusion echocardiography (RTMPE). Methods One hundred patients with STEMI were randomized into the following 2 groups: therapy (50 patients treated with sonothrombolysis and primary coronary angioplasty) and control (50 patients treated with primary coronary angioplasty). The patients underwent RTMPE for analysis of left ventricular ejection fraction (LVEF), wall motion score index (WMSI), and number of segments with myocardial perfusion defects 72 hours after STEMI and at 6 months of follow-up. P < 0.05 was considered statistically significant. Results Patients treated with sonothrombolysis had higher LVEF than the control group at 72 hours (50% ± 10% versus 44% ± 10%; p = 0.006), and this difference was maintained at 6 months of follow-up (53% ± 10% versus 48% ± 12%; p = 0.008). The WMSI was similar in the therapy and control groups at 72 hours (1.62 ± 0.39 versus 1.75 ± 0.40; p = 0.09), but it was lower in the therapy group at 6 months (1.46 ± 0.36 versus 1.64 ± 0.44; p = 0.02). The number of segments with perfusion defects on RTMPE was similar in therapy and control group at 72 hours (5.92 ± 3.47 versus 6.94 ± 3.39; p = 0.15), but it was lower in the therapy group at 6 months (4.64 ± 3.31 versus 6.57 ± 4.29; p = 0.01). Conclusion Sonothrombolysis in patients with STEMI resulted in improved wall motion and ventricular perfusion scores over time.

2.
Article in Chinese | WPRIM | ID: wpr-932390

ABSTRACT

Objective:To evaluate the feasibility and applicability of using phospholipid-hybridization method for preparing biomimetic microbubbles (Bio-MBs) ultrasound contrast agents.Methods:Leukocyte biomimetic microbubbles (MB leu), platelet biomimetic microbubbles (MB pla) and erythrocyte biomimetic microbubbles (MB ery) were prepared by multiple steps: film-hydration, phospholipid-hybridization, mechanical oscillation. The size and zeta potential of Bio-MBs were measured by dynamic light scattering. A laser scanning confocal microscopy experiment was performed to confirm the presence of membrane proteins on the shell of Bio-MBs. The fluorescence of FITC-labeled typical membrane protein was evaluated using a flow cytometer. Sodium dodecyl sulfate polyacrylamide gel electrophoresis was used to characterize the membrane protein. Biosafety of Bio-MBs was evaluated by CCK-8 counting kit, blood and major organs. The contrast enhancement effect and stability were observed in vitro and in vivo. An in vivo fluorescence imaging system was performed to evaluate the distribution of Bio-MBs. The application value of biomimetic microbubbles was measured by ultrasound molecular imaging by using ischemia-reperfusion rat models and acute hepatitis rat models. Results:Bio-MBs with spherical shape distributed homogenously, without obvious aggregation. The membrane proteins were successfully integrated into the shell of Bio-MBs.The diameter of three Bio-MBs was similar to that of control microbubbles (MB con) ( P>0.05), three Bio-MBs had a lower zeta potential than MB con ( P<0.05). The Bio-MBs had an appreciable performance in vitro and in vivo biosafety. The Bio-MBs retained the main proteins inherited from cell membrane. Contrast enhanced ultrasound imaging in vitro and in vivo showed that the Bio-MBs had a stable imaging ability.MB leu and MB pla have good targeted imaging effect in two disease models. Conclusions:A series of Bio-MBs ultrasound contrast agents, which have high stability, biosafety and targeted imaging efficiency, were successfully prepared by using phospholipid-hybridization method. This fabrication method for obtaining Bio-MBs can be applied to different clinical scenarios with different cell types in the future.

3.
Radiol. bras ; 54(5): 321-328, Sept.-Oct. 2021. graf
Article in English | LILACS-Express | LILACS | ID: biblio-1340580

ABSTRACT

Abstract Although contrast-enhanced ultrasound has been shown to provide considerable benefits, particularly in pediatric patients, it is still used relatively rarely in Brazil. It has proven to be a safe technique, and adverse effects are rare. In this review, we address the technique and main applications of contrast-enhanced ultrasound in the pediatric population, including the evaluation of focal liver lesions, abdominal trauma, kidney grafts, liver grafts, bowel loops, and vesicoureteral reflux. It is important for pediatric radiologists to be acquainted with this promising tool, understanding its applications and limitations.


Resumo A ultrassonografia com contraste de microbolhas é um método ainda pouco utilizado no Brasil, mas que tem grandes vantagens, ainda mais na faixa pediátrica, como ausência de radiação e dispensa de sedação/anestesia. O contraste de microbolhas tem se mostrado bastante seguro e com raríssimas reações adversas graves. Apresentaremos nesta revisão a técnica para o uso desse contraste na ultrassonografia, bem como as principais aplicações na faixa pediátrica, como avaliação das lesões hepáticas focais, do trauma abdominal, do enxerto hepático e renal nos transplantes, das alças intestinais e da pesquisa de refluxo vesicoureteral. É importante que radiologistas que exerçam funções em centros pediátricos conheçam essa ferramenta promissora.

4.
Acta Pharmaceutica Sinica ; (12): 761-770, 2021.
Article in Chinese | WPRIM | ID: wpr-876524

ABSTRACT

Blood-brain barrier (BBB) is the most challengeable obstacle for brain-targeted drug delivery. The combination of focused ultrasound (FUS) and microbubbles provides a new way for brain-targeted drug delivery across BBB based on the cavitation effect. This review briefly described the recent research of FUS combined with microbubbles to enhance the BBB permeability for brain-targeted drug delivery. The contents included the FUS mechanism, the types of the commercial microbubbles, shell materials, inner gas and preparation techniques of microbubbles, the opening mechanism of FUS with microbubbles, and the safety consideration. FUS combined with microbubbles may be the effective strategy to improve the BBB permeability for brain-targeted delivery, which could provide references for the clinical applications.

5.
Pesqui. vet. bras ; 41: e06785, 2021. tab, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1250490

ABSTRACT

The objective of this study was to verify the applicability of B-mode ultrasonography, ARFI elastography and CEUS in the diagnosis of chronic kidney disease and its Stages in dogs. 24 healthy dogs and 28 with CKD were included. In B-mode, the echogenicity, echotexture and cortico-medullary ratio of the kidneys were verified. By elastography, the shear-wave velocity of the cortical (SWVcort) and medullary (SWVmed) regions were determined and tissue deformity was evaluated. Wash-in, wash-out and peak enhancement (TPic) of the contrast in the renal parenchyma were calculated and homogeneity, presence of filling gaps and distinction of filling phases were evaluated by CEUS. Changes in echogenicity, echotexture and cortico-medullary ratio were observed only in sick patients. There was an increase in SWVcort in CKD, with a cutoff point >2.91m/s. Healthy kidneys were non-deformable and 25% had changes in gray scales. There was an increase in wash-in and TPic, changes in filling characteristics, filling failures and difficulty in distinguishing between the Stages in CEUS in CKD. It was found that dogs with CKD 2, 3 and 4 had greater SWVcort and wash-in values than CKD 1. Elastographic and CEUS changes were observed in dogs with CKD, demonstrating the applicability of ultrasonographic techniques in their diagnosis.(AU)


O objetivo deste estudo foi verificar a aplicabilidade da ultrassonografia modo-B, elastografia ARFI e CEUS no diagnóstico da doença renal crônica e seus estágios em cães. Foram incluídos 24 cães saudáveis e 28 com DRC. Pelo modo-B, verificou-se ecogenicidade, ecotextura e relação córtico-medular dos rins. Pela elastografia, foram determinadas as velocidades de cisalhamento das regiões cortical (SWVcort) e medular (SWVmed) e foi avaliada a deformidade tecidual. Calculou-se os temos de wash-in, wash-out e pico de intensidade (TPic) do parênquima renal e avaliou-se homogeneidade, presença de falhas e distinção das fases de preenchimento por contraste por meio de CEUS. Alterações em ecogenicidade, ecotextura e relação córtico-medular foram observadas somente em pacientes doentes. Houve aumento da SWVcort na DRC, com ponto de corte >2.91m/s. Rins saudáveis foram não-deformáveis e 25% apresentou alterações nas escalas de cinza. Houve aumento do wash-in e TPic, alterações nas características de preenchimento, falhas e dificuldade para distinguir as fases de preenchimento do contraste nos rins com DRC. Verificou-se que cães com DRC graus 2, 3 e 4 tiveram valores de SWVcort e wash-in maiores que cães com DRC grau 1. Foram obsrervadas alterações elastográfica e de CEUS em cães com DRC, demonstrando a aplicabilidade destas técnicas ultrassonográficas no seu diagnóstico.(AU)


Subject(s)
Animals , Dogs , Congenital Abnormalities , Ultrasonography , Renal Insufficiency, Chronic , Elasticity Imaging Techniques , Perfusion , Kidney
6.
J. vasc. bras ; 20: e20200093, 2021. tab, graf
Article in Portuguese | LILACS | ID: biblio-1346433

ABSTRACT

Resumo Contexto O ultrassom contrastado por microbolhas (CMUS) é uma modalidade diagnóstica de acurácia bem demonstrada por estudos internacionais para seguimento de reparo endovascular do aneurisma de aorta abdominal (EVAR). Não existem, no entanto, estudos nacionais focados nesse método de seguimento. Objetivos O objetivo deste estudo foi relatar a experiência inicial com CMUS em um hospital terciário, traçando uma comparação dos achados do CMUS com o ultrassom Doppler convencional (USGD), com o intuito de verificar se a adição de contraste ao protocolo padrão de controle ultrassonográfico incorreu alteração nos achados. Métodos Entre 2015 e 2017, 21 pacientes em seguimento de EVAR foram submetidos ao USGD seguido de CMUS. Foram avaliados os achados de exame referentes à identificação de complicações, bem como à capacidade de identificação da origem da endofuga. Resultados Entre os 21 casos avaliados, 10 complicações foram evidenciadas no total: sete pacientes apresentaram endofuga (33,3%); dois pacientes apresentaram estenose em ramo de endoprótese (9,52%); e um paciente apresentou dissecção em artéria ilíaca externa (4,76%). Em 21 pacientes avaliados, o uso combinado dos métodos identificou 10 casos de complicações pós-EVAR. Em seis dos sete casos de endofugas (85,71%), o uso dos métodos combinados foi capaz de identificar a origem. O USGD isolado falhou na identificação da endofuga em dois casos (28,5%), identificando achados duvidosos em outros dois casos (28,5%), que obtiveram definição diagnóstica após associação do CMUS. Conclusões O CMUS é uma técnica de fácil execução, a qual adiciona subsídios ao seguimento de EVAR infrarrenal.


Abstract Background Microbubble contrast enhanced ultrasound (CEUS) is an accurate diagnostic method for follow-up after endovascular abdominal aortic aneurysm repair (EVAR) that has been well-established in international studies. However, there are no Brazilian studies that focus on this follow-up method. Objectives The objective of this study was to report initial experience with CEUS at a tertiary hospital, comparing the findings of CEUS with those of conventional Doppler ultrasound (DUS), with the aim of determining whether addition of contrast to the standard ultrasonographic control protocol resulted in different findings. Methods From 2015 to 2017, 21 patients in follow-up after EVAR underwent DUS followed by CEUS. The findings of these examinations were analyzed in terms of identification of complications and their capacity to identify the origin of endoleaks. Results There was evidence of complications in 10 of the 21 cases examined: seven patients exhibited endoleaks (33.3%); two patients exhibited stenosis of a branch of the endograft (9.52%); and one patient exhibited a dissection involving the external iliac artery (4.76%). In the 21 patients assessed, combined use of both methods identified 10 cases of post-EVAR complications. In six of the seven cases of endoleaks (85.71%), use of the methods in combination was capable of identifying the origin of endoleakage. DUS alone failed to identify endoleaks in two cases (28.5%) and identified doubtful findings in another two cases (28.5%), in which diagnostic definition was achieved after employing CEUS. Conclusions CEUS is a technique that is easy to perform and provides additional support for follow-up of infrarenal EVAR.


Subject(s)
Humans , Male , Aged , Aged, 80 and over , Aortic Aneurysm, Abdominal/surgery , Ultrasonography, Doppler/instrumentation , Contrast Media , Microbubbles , Endovascular Procedures , Follow-Up Studies , Aortic Aneurysm, Abdominal/rehabilitation , Aortic Aneurysm, Abdominal/diagnostic imaging , Ultrasonography, Doppler/methods
7.
Article in Chinese | WPRIM | ID: wpr-845211

ABSTRACT

Because of the blood-brain barrier (BBB)and the lack of significant enhancement method for the drug penetration into and retention in brain, effective methods and drugs for the treatment of brain diseases are extremely limited. Ultrasound can effec tively, safely and reversibly open the BBB and thus is an ideal method for the targeted, non-invasive drug entry into the intracranial tis sue, which has been expected to become a new means for treating brain diseases in the near future. Following a brief introduction of some biological characteristics of brain, this review elaborates the mechanisms of the ultrasound combining microbubbles(USMB)for brain targeted drug delivery, including the opening of the tight junction, stimulation of endocytosis, inhibition of multidrug resistance protein expression, enhancement of drug concentration in target area, extension of the residence time, and adjustment of the intersti tial pressure in target tissue. Then, the application of ultrasound in the treatment of some diseases including the Alzheimer's disease (AD), Huntington's disease(HD), Parkinson's disease(PD), and brain tumors are summarized, and the existing problems in the ul trasound therapy are also discussed, in order to provide a valuable reference for further improvement in the treatment of brain diseases.

8.
Article in Chinese | WPRIM | ID: wpr-860929

ABSTRACT

Objective: To observe the value of targeted contrast enhanced ultrasound (CEUS) using anti-Mullerian canal hormone (AMH) targeted nanobubbles (AMH-NB) for in vivo quantitative evaluation on ovarian neovascular density after ovarian auto-transplantation in SD rats. Methods: The nanobubbles carrying anti-AMH antibody were prepared, and their basic physical properties were examined. Then ovarian auto-transplantation rat models were established. The targeted (AMH-NB), non-targeted (N-NB) contrast agents and SonoVue were administered on the 7th day after transplantation to obtain peak intensity (PI) and time to peak (TTP). The microvascular density (MVD) was measured using immunohistochemistry, and the correlation of PI, TTP and MVD were analyzed. Results: The particle size of AMH-NB uniformly distributed, ranged (622.67±33.65)nm, and the concentration of AMH-NB was (2.90±0.26)×108/ml. PI of ovarian angiography with AMH-NB was (7.93±0.65)dB and TTP was (42.53±1.74)s, with N-NB was (6.14±0.44)dB and (54.35±1.73)s, with Sonovue was (4.15±0.83)dB and (28.71±1.18)s, respectively (all P<0.05).Immunohistochemical analysis showed that the microvascular density was (61.20±6.84)/HP, histological analysis indicated that AMH-NB were able to penetrate blood vessels to the interstitial space and combine with AMH. PI, TTP of AMH-NB were highly both correlated with MVD (r=0.84, r=-0.84, both P<0.05). Conclusion: AMH-NB can be used to qualitatively and quantitatively evaluate the angiogenesis in transplanted ovarian of rats in vivo with CEUS.

10.
Article in Chinese | WPRIM | ID: wpr-862176

ABSTRACT

Objective: To investigate the impact of diagnostic ultrasound (DUS) with different mechanical index (MI) combined with microbubbles on tumor blood flow and chemotherapeutic drug concentration in nude mice bearing human pancreatic cancer. Methods: Pancreatic tumor models were established through injecting tumor cell suspension into bilateral hind limbs in 33 nude mice,which were divided into three groups (group A, B and C) randomly. Contrast-enhanced ultrasound (CEUS), chemotherapeutic agents injection and exposure/sham exposure with DUS were performed on all models in the three groups. For each model, one of the hind limb was chosen as therapeutic side (exposure), and the other hind limb as control side (sham exposure). CEUS was performed before and after ultrasonic exposure/sham to obtain the time-intensity curve (TIC), peak intensity (PI) and AUC. Doxorubicin was injected through the tail vein before ultrasonic exposure/sham exposure. And microbubbles were continuously injected during ultrasonic exposure/sham exposure. MI of therapeutic side for ultrasonic exposure in group A, B and C was 0.3, 0.7 and 1.1, respectively. After ultrasonic exposure/sham exposure, Doxorubicin concentration in vitro tumor tissue was detected, and then histological analysis was done. In addition, the range of peak negative pressure correspond with different MI were detected before ultrasonic exposure/sham exposure. Results: Concentration of Doxorubicin of therapeutic side in group A was obviously higher than that of control side ([1.45±0.53]μg/g vs [1.07±0.46]μg/g; t=-5.163, P=0.001). In group B and C, there was no statistically significant difference of Doxorubicin concentration between therapeutic side and control side in the drug concentration (Z=-0.297, -0.357, P=0.766, 0.721). Quantitative analysis of CEUS showed that both PI and AUC increased obviously after ultrasonic exposure/sham exposure compared with those before exposure/sham exposure (all P<0.05), except for PI of control side in group B and AUC of control side in group B and C. Neither hemorrhage nor swelling was observed in tumor tissue of both sides in any group. The range of peak negative pressure correspond with MI of 0.3 (group A), 0.7 (group B) and 1.1 (group C) was 0.81-0.83 MPa, 0.96-1.32 MPa and 2.29-2.53 MPa, respectively. Conclusion: DUS (MI=0.3) combined with microbubbles can enhance blood perfusion and improve the chemotherapeutic drug concentration of tumor tissues in nude mice bearing human pancreatic cancer.

11.
Article in Chinese | WPRIM | ID: wpr-862078

ABSTRACT

As a non-invasive thermal ablation technique, high intensity focused ultrasound (HIFU) has been widely used in treatment of uterine fibroids with affirmed efficacy. Microbubble contrast agent has important clinical value in the visualization of lesions, the evaluation of efficacy after HIFU ablation and the mechanism of synergy. The advancements of microbubble contrast agent in treatment of uterine fibroids with HIFU were reviewed in this article.

12.
Article in Chinese | WPRIM | ID: wpr-861233

ABSTRACT

Objective: To investigate the effect of ultrasound-mediated soluble programmed cell death 1 receptor (sPD-1) and miR-206 loaded nanoscale microbubbles on H22 hepatoma subcutaneous xenografts in mice. Methods: sPD-1 and miR-206 loaded nanoscale microbubbles were prepared. The mice models of H22 hepatoma xenografts were established and randomly divided into model group, microbubble control group, miR-206 microbubble group, sPD-1 microbubble group and combined group (miR-206 and sPD-1 microbubbles). Mice in each group (each n=8) were treated with normal saline or corresponding nanoscale microbubbles every 2 days by injection via tail vein, and then irradiated by ultrasound once after every injection. Tumor tissues were obtained after being treated 5 times. Tumor volume and quality were measured, the volume and quality inhibitory rates were calculated. HE staining was used to observe pathological changes of the tumors. The expressions of Bcl-2, Bax proteins were detected by immunohistochemistry. The expressions of Bcl-2, Bax, c-met, interferon-γ (IFN-γ) and programmed cell death 1 receptor ligand (PD-L1) mRNA were detected with RT-PCR. Quantitative real-time fluorescence PCR was used to detect the expression of miR-206.Results: The nanoscale bubbles were spherical and distributed uniformly. Compared with model group, tumor volume and quality decreased in other groups, and the volume and quality inhibitory rates increased (all P<0.05), especially in combined group (all P<0.05). Compared with model group, the Bax protein and mRNA expressions both increased, whereas the Bcl-2 protein and mRNA expressions decreased in other groups, especially in combined group (all P<0.01). There were significant differences of Bax, Bcl-2, c-met, PD-L1, IFN-γ and miR-206 mRNA in tumor tissues among each group (all P<0.01). Conclusion: Ultrasound-mediated sPD-1 combine miR-206 loaded nanoscale microbubbles can synergistically inhibit H22 hepatoma xenografts in mice.

13.
Article in Chinese | WPRIM | ID: wpr-754796

ABSTRACT

To investigate the effects of ultrasound combined with microbubbles on intracellular Ca2+ homeostasis in carboplatin ( CBP )‐treated A549 cell and its possible mechanisms of inhibiting A549 cell line activity . Methods According to whether SonoVue was used or not ,and the different dose of CBP ,the groups A‐F were arranged as the ultrasound( US) group( group A ) ,the ultrasound combined with microbubbles ( USMB) group( group B) ,the low dose CBP ( 100 μg/ml) + US group( group C) ,the low dose CBP+USMB group( group D) ,the high dose CBP ( 200 μg/ml)+ US group ( group E) and the high dose CBP+USMB group( group F) .A549 cells were bathed and washed by a calcium‐free buffer , loaded with Ca2+ indicator fluo‐4 AM . Real‐time images were acquired using laser confocal microscopy . T he fluorescence intensity of intracellular calcium ion concentration ( [ Ca 2+ ] i ) in individual living cell was observed and the calcium overload was analyzed . Results After ultrasound irradiation ,the normalized fluorescence intensity of [ Ca2+ ] i increased rapidly ,then returned to a new homeostasis ( selected cells in groups A ,B ,E ,F ) or experienced a second calcium oscillation ( some cells in group C and D ) . All the selected cells in group B and some cells in group C and D exhibited superimposed oscillations . T he calcium overloading time in group D was longer than those of any other groups . Four cells in group A experienced delayed calcium oscillations . Compared with group A ,the selected cells in other groups exhibited a larger amplitude of calcium oscillation ( all P < 0 .05 ) and the selected cells in group B and D exhibited calcium oscillation for a longer period of time( all P <0 .05) . Conclusions In the calcium‐free buffer ,US ,USMB , CBP+ US ,CBP + USMB are direct stimuli of calcium overload in A 549 cells . SonoVue ,CBP ,CBP +SonoVue are all synergistic stimuli of calcium overload in A 549 cells irradiated by ultrasound .US ,USMB and CBP may synergistically induce calcium release from intracellular store sites in A 549 cells . Calcium overload is a possible mechanism of ultrasound combined with microbubbles in assisting CBP chemotherapy .

14.
Article in Chinese | WPRIM | ID: wpr-745154

ABSTRACT

Objective To investigate the effects of ultrasound combined with microbubbles on intracellular Ca2+ homeostasis in carboplatin ( CBP )-treated A549 cell and its possible mechanisms of inhibiting A549 cell line activity . Methods According to whether SonoVue was used or not ,and the different dose of CBP ,the groups A-F were arranged as the ultrasound(US) group(group A) ,the ultrasound combined with microbubbles ( USMB) group( group B) ,the low dose CBP ( 100 μg/ml) + US group( group C) ,the low dose CBP+USMB group( group D) ,the high dose CBP ( 200 μg/ml)+ US group ( group E) and the high dose CBP+USMB group( group F) .A549 cells were bathed and washed by a calcium-free buffer , loaded with Ca2+ indicator fluo-4 AM . Real-time images were acquired using laser confocal microscopy .The fluorescence intensity of intracellular calcium ion concentration ([Ca2+ ]i) in individual living cell was observed and the calcium overload was analyzed . Results After ultrasound irradiation ,the normalized fluorescence intensity of [Ca2+ ]i increased rapidly ,then returned to a new homeostasis (selected cells in groups A ,B ,E ,F) or experienced a second calcium oscillation ( some cells in group C and D) . All the selected cells in group B and some cells in group C and D exhibited superimposed oscillations . The calcium overloading time in group D was longer than those of any other groups . Four cells in group A experienced delayed calcium oscillations . Compared with group A ,the selected cells in other groups exhibited a larger amplitude of calcium oscillation( all P < 0 .05) and the selected cells in group B and D exhibited calcium oscillation for a longer period of time( all P <0 .05) . Conclusions In the calcium-free buffer ,US ,USMB , CBP+ US ,CBP + USMB are direct stimuli of calcium overload in A 549 cells . SonoVue ,CBP ,CBP +SonoVue are all synergistic stimuli of calcium overload in A 549 cells irradiated by ultrasound .US ,USMB and CBP may synergistically induce calcium release from intracellular store sites in A 549 cells . Calcium overload is a possible mechanism of ultrasound combined with microbubbles in assisting CBP chemotherapy .

15.
Article in Chinese | WPRIM | ID: wpr-745139

ABSTRACT

Objective To explore the application value of IL-8 monoclonal antibody microbubble combined with ultrsound targeted microbubble destruction ( UTMD) on alleviating myocardial ischemia reperfusion/injury ( MIRI) in rabbits .Methods Forty-two rabbits were randomly divided into closed chest group ( n =7) ,open chest control group ( n = 7) and ischemia-reperfusion ( I/R) group ( n = 28) .I/R group were randomly divided into 30 min reperfusion group( n =7) ,60 min reperfusion group( n =7) ,120 min reperfusion group ( n = 7 ) and 180 min reperfusion group ( n = 7 ) .All rabbits were examined by electrocardiogram , echocardiography and HE staining after MIRI . Targeted myocardial contrast echocardiography ( MCE) was performed and ELISA was used to detect IL-8 content in rabbit myocardium before and after UTMD . Results Electrocardiogram and wall motion returned to normal at 60 min after reperfusion .Targeted MCE showed that with the prolongation of reperfusion after I/R ,the video intensity of myocardium in reperfusion area increased gradually , reaching its peak at 120 min and 180 min after reperfusion .After UTMD ,the video intensity decreased ,and the change rate of video intensity in 30 min reperfusion group was higher than those in other reperfusion groups(all P<0 .05) .The content of IL-8 and its neutralization rate in the ELISA results were consistent with the video intensity and rate of change of targeted MCE .HE staining and scanning electron microscopy showed that myocardial injury was found in I/R group .With the prolongation of reperfusion time ,the degree of myocardial injury was gradually aggravated ,and the injury was alleviated after irradiation .Conclusions IL-8 monoclonal antibody combined with UTMD has the advantages of non-invasive and highly effective in alleviating MIRI .It provides a new way to treat MIRI .

16.
Article in Chinese | WPRIM | ID: wpr-745138

ABSTRACT

Objective To prepare a novel H2 O2-loaded and phase-change nanoparticles ,combined with low intensity focused ultrasound (LIFU) to dissolve intravascular thrombosis for the evaluation of its value in the treatment of coronary artery thrombolysis in the patients with acute myocardial infarction ( AMI) . Methods H2 O2-loaded and phase-change nanoparticles were prepared by membrane hydration method . 50 ml arterial blood was collected from rabbit carotid artery and made into 400-800 mg thrombus blocks . They were divided into four groups randomly :poly butylene succinate buffer ( PBS) solution as blank control (group A) ,SonoVue microbubbles group(group B) ,PFP phase-change nanoparticles group ( group C ) , H2 O2-loaded and phase-change nanoparticles group ( group D ) . Each group of thrombus samples were placed into the artificial blood vessels of the extracorporeal thrombolysis simulation cycle device and combined with LIFU irradiation . The weights and pathological presentations were compared before and after thrombolysis . The volume of O2 in the vascular system was inspected using Dissolved Oxygen Detector ( DOD) after LIFU exposure . Results The average size of H2 O2-loaded nano-droplets was ( 456 .7 ± 31 .2) nm . Fluorescence microscopy showed that the structure of nanoparticles was stable and uniform in size . The weight loss and rates of thrombus in the four groups after thrombolysis were:group A (52.2±11.5)mg,(9.7±3.5)% ;groupB(110.0±21.9)mg,(19.4±2.4)% ;groupC(239.6±46.3)mg, (39 .7 ± 6 .3)% and group D (309 .8 ± 44 .8)mg ,(54 .2 ± 6 .6)% ,respectively . The weight loss and rates of thrombus in group C and D were higher than those in group A and B ( all P < 0 .01) ,but there was no significant difference between group C and D ( P >0 .05) . The DOD showed that along with the increase of H2O2 concentration ,O2 release was rising . Conclusions H2O2/PFP nanoparticles with LIFU performs efficient thrombolysis and provides O2 to improve hypoxia ,which provides a potential novel treatment method for AMI patients with coronary embolism .

17.
Journal of Chinese Physician ; (12): 235-238, 2019.
Article in Chinese | WPRIM | ID: wpr-744858

ABSTRACT

Objective To investigate whether the low intensity pulsed ultrasound combined the microbubbles can increase the delivery of Doxorubicin in tumor.Methods 24 tumor-bearing rabbits were divided into 3 groups randomly,namely,8 rabbits in the ultrasound microbubbles adriamycin group (Ad-US-MB),8 rabbits in the adriamycin group (Ad) and 8 rabbits in the blank control group.The animals were intravenous injected with doxorubicin hydrochloride,and then the tumor of the animals were disposed by low-intensity ulrtasound and mirobubbles for 10 minutes.Then these rabbits in each group were sacrificed immediately after treatment.To observe the spatial distribution of Adriamycin and microvessel of tumors,and to quantitatively compare the changes of Adriamycin concentration between the two groups.Results The Adriamycin concentration in Ad-US-MB group [(0.682 ±0.0001) μg/ml] was higher than that in Ad group [(0.422-± 0.357) μg/ml] (P < 0.05).The distribution of immunofluorescence in Ad-US-MB group was observed.Adriamycin was densely distributed around the microvessels of tumors,even farther away.In Ad group,only sparsely distributed adriamycin was found around the microvessels of tumors.The concentration distribution of adriamycin was not found in the blank control group without Doxorubicin injection.Conclusions The pulsed low frequency ultrasound combined with microbubbles can increase the permeability of chemotherapy drug.

18.
Article in Chinese | WPRIM | ID: wpr-743773

ABSTRACT

Objective Analysis of the effect and the mechanism of adenovirus with down regulation of matrix metalloproteinase inhibitor 1 (TIMP-1) achieved targeting by ultrasound microbubbles combined with ultrasound irradiation for liver fibrosis in rats. Methods Recombinant adenovirus-mediated with down regulation of TIMP-1 gene was constructed and a mixture of recombinant adenovirus and ultrasound contrast agent was prepared.The rat liver fibrosis model was established and divided into 5 groups : model group; adenovirus group (recombinant adenovirus); adenovirus microbubble group (mixture of recombinant adenovirus and ultrasound contrast agent); experimental group (ultrasound irradiation + mixture of recombinant adenovirus and ultrasound contrast agent); ultrasound adenovirus group (ultrasound irradiation + recombinant adenovirus). The rats were sacrificed after 24 hours and liver sections were prepared. The expression of EGFP in each group was observed and the transfection rate was analyzed. The liver slices were stained by Masson to judge the stage of liver fibrosis. ANOVA analysis was used to compare the levels of alanine aminotransferase (ALT) , aspartate aminotransferase (AST) , bydroxyproline (Hyp) , hyaluronic acid (HA) , type IV collagen (CIV) and laminin (LN) in each group. The relative expression levels of TIMP-1 and matrix metalloproteinase 13 (MMP-13) in each group were detected by Western blot. Results The transfection rate of the experimental group was higher than that of the adenovirus group (q = 3.418) , the adenovirus microbubble group (q = 3.756) and the ultrasonic adenovirus group (q = 5.502) , and the difference was statistically significant (P < 0.05); Pathological examination showed that the degree of fibrosis in the experimental group and the grade of liver fibrosis were lower than the other groups (P < 0.01). The activities of ALT, AST, HA, LN, CIV and Hyp in the experimental group were lower than those in the other 4 groups.Western blot showed that the level of TIMP-1 protein expression was highest while the level of MMP-13 protein expression was lowest in the experimental group than those in the other groups. Conclusion Adenovirus with down regulation of TIMP-1 achieved targeting by ultrasound microbubbles combined with ultrasound irradiation can inhibit the activity of TIMP-1 and improve the degree of liver fibrosis. Gene therapy is an potential therapeutic method in the application of treating liver fibrosis.

19.
Rev. MVZ Córdoba ; 23(1): 6552-6563, Jan.-Apr. 2018. graf
Article in English | LILACS-Express | LILACS | ID: biblio-957352

ABSTRACT

ABSTRACT This study describes the ultrasonographic techniques currently used in the evaluation of the canine pancreas. Ultrasonography was the first method to enable direct visualization of the pancreas in humans and it has been subsequently applied to animals. Currently, it is the method of choice for pancreatic evaluation and is essential as a diagnostic tool in the detection of abnormalities, especially tumors. Innovative equipment technology has led to the emergence of techniques complementary to B-mode ultrasound; such as Doppler, elastography, and contrast-enhanced ultrasonography, which have enabled more accurate diagnosis. Doppler provides information on vascular architecture and the hemodynamic aspect of blood vessels in multiple organs. ARFI elastography provides detailed images of the alterations detected by conventional examination (qualitative method) and assists in differentiating between benign and malignant processes (quantitative method). Microbubble contrast agents determine parameters related to homogeneous and heterogeneous filling of organs with microbubbles, mainly nodular areas, thus defining high and low intensity patterns.


RESUMEN Este estudio describe las técnicas ecográficas frecuentemente utilizadas para evaluar el pancreas del perro. La ecografía fue el primer método que permitió la visualización directa del páncreas en seres humanos y que luego se aplicó en animales. Es actualmente el método de elección para la evaluación del páncreas y es esencial como herramienta diagnóstica en la detección de anomalías, especialmente tumores. La tecnología innovadora de los equipos, llevó a la aparición de técnicas complementarias al modo B, tales como Doppler, elastografía, ecografía de contraste, que han permitido realizar diagnósticos más precisos. El Doppler proporciona información sobre la arquitectura vascular y aspectos hemodinámicos de los vasos sanguíneos en múltiples órganos. La elastografía ARFI ofrece imágenes detalladas de las alteraciones detectadas en exámenes convencionales (método cualitativo) y ayuda a diferenciar entre procesos benignos y malignos (método cuantitativo). Los agentes de contraste con microburbujas permiten determinar parámetros relacionados con el llenado homogéneo o heterogéneo de los órganos, principalmente áreas nodulares, definiendo, por tanto, patrones de alta o baja intensidad.

20.
J. bras. nefrol ; 40(1): 86-90, Jan.-Mar. 2018. graf
Article in English | LILACS | ID: biblio-893820

ABSTRACT

Abstract Renal oncocytoma is an infrequently reported renal neoplasm, often asymptomatic, which usually behaves as a benign entity and is identified accidentally on radiological imaging. Transplant patients under long-term immunosuppressive drugs have a high prevalence of cancers, such as skin cancers, lymphoproliferative disorders, and renal carcinomas. We present a case report of an asymptomatic renal oncocytoma in a kidney transplant recipient presenting persistent hematuria. The features of computed tomography and contrast-enhanced ultrasound (CEUS) are presented. This was the first time we used CEUS in a transplant kidney recipient presenting a renal mass, allowing the real-time visualization of contrast-enhancement patterns during all vascular phases for the differential diagnosis of renal tumors. Although the pattern of intense vascularization could mislead to an early judgment as a malignant lesion, it could help to exclude other renal lesions without inducing nephrotoxicity.


Resumo O oncocitoma renal é uma neoplasia renal raramente relatada, muitas vezes assintomática, que geralmente se comporta como uma entidade benigna e é identificada acidentalmente em imagens radiológicas. Pacientes transplantados em regimes imunossupressores de longa duração apresentam alta prevalência de neoplasias tais como câncer de pele, distúrbios linfoproliferativos e carcinomas renais. Apresentamos o relato de um caso de oncocitoma renal assintomático em receptor de transplante renal com hematúria persistente. São apresentados os achados de imagens de tomografia computadorizada e ultrassonografia (US) com contraste. Foi a primeira vez que utilizamos a US com contraste em um receptor de transplante renal que apresentava massa renal, permitindo a visualização em tempo real dos padrões de realce do contraste em todas as fases vasculares para o diagnóstico diferencial dos tumores renais. Embora o padrão de vascularização intensa possa induzir uma avaliação precoce de lesão maligna, o exame ajuda a excluir outras lesões renais sem induzir nefrotoxicidade.


Subject(s)
Humans , Male , Middle Aged , Postoperative Complications/diagnostic imaging , Kidney Transplantation , Adenoma, Oxyphilic/diagnostic imaging , Contrast Media , Kidney Neoplasms/diagnostic imaging , Ultrasonography/methods
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