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1.
Article in Chinese | WPRIM | ID: wpr-879138

ABSTRACT

In this paper, network pharmacology method and molecular docking technique were used to investigate the target genes of Olibanum and Myrrha compatibility and the possible mechanism of action in the treatment of rheumatoid arthritis(RA). Our team obtained the main active components of Olibanum-Myrrha based on literatures study, relevant traditional Chinese medicine systematic pharmacological databases and literature retrieval, and made target prediction of the active components through SwissTargetPrediction database. At the same time, RA-related targets were collected through DrugBank, GeneCards and Therapeutic Target Database(TDD) databases; and VENNY 2.1 was use to collect intersection targets to map common targets of drug and disease of Venn diagram online. The team used STRING database to construct PPI protein interaction network diagram, and screen out core targets according to the size of the interaction, and Cytoscape 3.6.0 software was used to construct network models of "traditional Chinese medicine-component-target" "traditional Chinese medicine-component-target-disease" and core target interaction network model. The intersection target was analyzed by using DAVID 6.8 online database for GO function analysis and KEGG pathway enrichment analysis, and Pathon was used to visualization. AutoDock Vina and Pymol were used to connect the core active components with the core targets. Sixteen active components of Olibanum-Myrrha pairs were found and collected in the laboratory, and 320 relevant potential targets, 468 RA-related targets and 62 intersection targets were obtained through the Venn diagram. It mainly acted on multiple targets, such as IL6, TNF, IL1 B and MAPK1, involving TNF signaling pathway and Toll-like receptor signaling pathway in RA treatment. Finally, in this study, possible targets and signaling pathways of Olibanum-Myrrha compatibility therapy for RA were discussed, and molecular docking between core targets and core active components was conducted, which could provide scientific basis for the study on the mechanism of Olibanum-Myrrha compatibility.


Subject(s)
Arthritis, Rheumatoid/genetics , Drugs, Chinese Herbal , Frankincense , Humans , Medicine, Chinese Traditional , Molecular Docking Simulation
2.
Article in Chinese | WPRIM | ID: wpr-879015

ABSTRACT

The volatile oil from Mastiche and Olibanum medicinal materials was extracted by steam distillation, and the chemical components of the volatile oil were analyzed by GC-MS technology. The differences of the volatile oil components were compared and study on the Helicobacter pylori in vitro antimicrobial activitiy was conducted. The results showed that the yields of the volatile oil from Mastiche and Olibanum were 11.93% and 2.40%, respectively. A total of 46 compounds(91.31%) were identified from the volatile oil from Mastiche annd 35 compounds(92.49%) from Olibanum. The classification and comparison study of the components showed that the content of monoterpenes in the volatile oil from Mastiche was the highest(40.69%), followed by alcohols(28.48%); while the content of alcohols in the volatile oil from Olibanum was the highest(35.81%), followed by esters(24.92%). There were significant differences in the components of volatile oil from Mastiche and Olibanum, which might be one of the reasons for the difference in efficacy and application. In vitro bacteriostatic experiments showed that the minimum inhibitory concentration(MIC) of the volatile oil from Mastiche against H. pylori was 1 mg·mL~(-1), and the MIC of the volatile oil from Olibanum against H. pylori was more than 1 mg·mL~(-1). In combination with the results of the oil yield experiment, Mastiche had the advantage of inhibiting H. pylori activity. The research results provide scientific basis for the rational application of Mastiche and Olibanum.


Subject(s)
Anti-Bacterial Agents/pharmacology , Frankincense , Gas Chromatography-Mass Spectrometry , Helicobacter pylori , Monoterpenes/analysis , Oils, Volatile/pharmacology
3.
Article in Chinese | WPRIM | ID: wpr-921801

ABSTRACT

Five compounds were isolated from the alcohol extract of Olibanum by MCI, silica gel, ODS, and Sephadex LH-20 column chromatographies and preparative high-performance liquid chromatography(HPLC). On the basis of spectral data and literature data, the compounds were identified as:(1S,3R,4S,7R,11S,12R)-1:12,4:7-diepoxisonane-8(19)-ene-3,11-diol(1), boscartin A(2),(+)-resinolin(3),(+)-5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one(4), and acerogenin A(5). Compound 1 is a new compound, and compounds 3-5 were isolated from Olibanum for the first time. The structure of compound 1 was determined by spectroscopic analysis and single-crystal X-ray diffraction. Compounds 1 and 2 were tested for PC12 neurotoxicity, and the results showed that they were both safe compounds.


Subject(s)
Chromatography, High Pressure Liquid , Diterpenes , Frankincense , Molecular Structure
4.
Article in Chinese | WPRIM | ID: wpr-921752

ABSTRACT

Two terpenes, 3-keto-tirucalla-8,24-dien-21-oic acid(KTDA) and 2-methoxy-5-acetoxy-furanogermacr-1(10)-en-6-one(FSA), are isolated from Olibanum and Myrrha respectively, which are characterized by high yield and easy crystallization during the preparation. The present study explored the regulatory targets and anti-inflammatory mechanism of KTDA and FSA based on network pharmacology and cell viability assay. First, the drug-likeness of KTDA and FSA was predicted by Swiss ADME. The target prediction of active components was carried out by Swiss Target Prediction and Pharmmapper. TTD, Drug Bank, and Gene Cards were searched for inflammation-related target genes of KTDA and FSA. Protein-protein interaction(PPI) analysis was performed on the inflammatory targets of KTDA and FSA by STRING, and Cytoscape was used to conduct topological analysis of the interaction results and construct the PPI network. GO function and KEGG pathway enrichment analyses of inflammatory targets of KTDA and FSA were carried out by DAVID, and a " component-target-pathway" network was constructed. Finally, lipopolysaccharide(LPS)-induced RAW264. 7 cells were treated with KTDA and FSA at different concentrations, and nitric oxide(NO) concentration and protein and m RNA expression levels were detected. The results showed that both KTDA and FSA showed good drug-likeness. A total of 157 and 142 inflammation-related targets of KTDA and FSA were screened out. PPI network analysis showed that MAPK1, AKT1, MAPK8, PIK3 CA,PIK3 R1, EGFR, etc. might be the key proteins for the anti-inflammatory effect. PI3 K/AKT and MAPK signaling pathways were obtained by KEGG and GO-BP enrichment. Cell experiment results showed that KTDA and FSA could exert anti-inflammatory effects by inhibiting NO production, reducing the phosphorylation levels of JNK, p38, and AKT proteins, and down-regulating the m RNA expression of interleukin(IL)-1β and IL-6. Meanwhile, FSA could also inhibit ERK phosphorylation. The results indicated that KTDA and FSA had significant anti-inflammatory activity, which provided a scientific basis and important support for the further research,development, and utilization of Olibanum and Myrrha.


Subject(s)
Animals , Ants , Drugs, Chinese Herbal/pharmacology , Frankincense , Lipopolysaccharides , Molecular Docking Simulation
5.
Article in Chinese | WPRIM | ID: wpr-846558

ABSTRACT

Objective: To optimize the extraction and purification process of the boswellic acids components in the frankincense. Methods: The extraction amount and extract yield of 13 boswellic acids (3-oxotirucall-8,24-dien-21-oic acid, 3α-acetoxy-tirucall-7, 24-dien-21-oic acid, 3-hydroxytirucall-8,24-dien-21-oic acid, acetyl 11α-methoxy-β-boswellic acid, 3α-hydroxy tirucall-7,24-dien- 21-oic acid, 11-keto-boswellic acid, 3-O-acetyl-α-boswellic acid, 3α-acetyloxylanosta-8,24-dien-21-oic acid, 3β-acetoxy-5α-lanosta- 8,24-dien-21-oic acid, 3-acetyl-11-keto-β-boswellic acid, 3-acetyloxy-tirucall-8,24-dien-21-oic acid, α-boswellic acid, β-boswellic acid) in frankincense were detected by UPLC-TQ/MS, the extraction method was investigated by single factor and response surface, the extraction solvent, ratio of solid to liquid, extraction time and extraction times were investigated on the extraction process, and the extract was purified by alkali dissolving acid precipitation method and the purification process parameters were investigated by single factor and orthogonal test to determine the best purification process. Results: The optimum condition for the extraction of the frankincense is that twenty times of 95% ethanol for four times reflux extraction and 62 min for each time. Optimum purification process was as following: Dissolve in lye pH 12-13, and the solution was precipitated at 0-4 ℃ with pH < 2 for 30 min. The purity of boswellic acids was 73.87%. Conclusion: The optimized extraction and purification process is stable and feasible, which is suitable for the extraction and purification of effective fraction of frankincense and beneficial to give full play to the medicinal value of frankincense and provide scientific basis for material basis research of frankincense.

6.
Article in Chinese | WPRIM | ID: wpr-846064

ABSTRACT

As a commonly used traditional Chinese medicine, chemical components of olibanum are mainly triteterpenes, diteterpenes, monoterpenes, which exhibit a range of biological activities including anti-inflammatory, anti-tumor, hepatoprotective, antibacterial, antifibrosis and so on. It is commonly used in the treatment of rheumatism and rheumatoid arthritis. In this review, the chemical constituents and pharmacological activities of olibanum are summarized in order to provide a reference for the further development and rational utilization of this plant resource.

7.
Article in Chinese | WPRIM | ID: wpr-878815

ABSTRACT

As a representative foreign medicinal material, olibanum(Ruxiang) was imported to China since the Qin and Han Dynasties. Olibanum was first described as a medicinal by the name "Xunluxiang" in Miscellaneous Records of Famous Physicians(Ming Yi Bie Lu). This study investigated historical records on olibanum and conducted the herbalogical study. It was found that olibanum came from the resin mainly obtained from the bark of Pistacia lenticus before the Tang Dynasty. With the prosperity of the Maritime Silk Road, instead, the resin obtained from the bark of Boswellia carterii was mainly used as olibanum. In ancient time, the oleo-gum-resin secreted from the cut bark was collected in spring and summer, and the quality was judged based on transparency and shape. The processing methods of olibanum went through many evolutions, which changed from simple methods such as grinding and frying to complex methods such as levigating and grinding with wine, and now to frying and processing with vinegar. The usage of olibanum included alchemy, folk and religious incense, bathing, cosmetic and medicinal since ancient times. From the Song Dynasty, olibanum had been mainly used as medicinal because of its good effect to treat wounds. In traditional Chinese medicine, olibanum unblocks menstruation, relieves pain and reduces swelling and generated muscles. The medicinal efficacy of olibanum is not much different from ancient to modern. Only the efficacy of replenishing energy and promoting the movement of Qi was rarely mentioned in modern reference. In this article, the historical evolutions of olibanum about original plants, processing and medicinal efficacy were sorted out. The results could provide historical basis for the further development and clinical utilization of olibanum.


Subject(s)
China , Drugs, Chinese Herbal , Frankincense , Medicine, Chinese Traditional , Resins, Plant
8.
China Pharmacist ; (12): 1007-1010, 2018.
Article in Chinese | WPRIM | ID: wpr-705649

ABSTRACT

Objective: To establish the HPLC fingerprint of Olibanum. Methods: The column was Agilent ZORBAX SBC18(250 mm×4. 6 mm,5 μm) with the mobile phase of acetonitrile-0. 1% phosphoric acid (gradient elution) at a flow rate of 1. 0 ml·min-1, the detection wavelength was 270 nm,the column temperature was 30℃,and the injection volume was 10 μl. Olibanum samples from different regions were detected for the characteristic fingerprint. Similarity evaluation software for chromatographic fingerprint of tradi-tional Chinese medicine (2012 edition) was used for the common peaks identification and similarity evaluation. Results: The HPLC fingerprint analysis method for Olibanum was established. The fingerprint consisted of 10 common peaks. The similarities of the finger-prints of twelve samples from different regions were above 0. 95. Conclusion: The method is simple and rapid with good reproducibili-ty, which provides basis for the quality control and evaluation standard of Olibanum.

9.
Article in Chinese | WPRIM | ID: wpr-852872

ABSTRACT

Objective: To optimize the technology and evaluate the influence of micronization on Olibanum physic-chemical characteristics and dissolution. Methods: The single factor test was employed to investigate the effect of the size and freezing time of common powder particle, the ratio of excipients, and grinding time on the yield of ultramicro powder; Additionally a comparative research between ultramicro powder and common powder was designed, including micromeritic properties (angle of repose, bulk density, tap density, and squeezing degree), effective components concentration [volatile oil, octyl acetate and 3-acetyl-11-keto-β-boswellic acid (AKBA)], and cumulative dissolution rate. Results: The optimal technology of ultramicro powder was as follows: the medicinal material was ground into fine powder, followed by 4 h freezing at -20℃ and 15 min superfine grinding with excipient and fine powder in ratio of 1:5; As the degree of smashing enhanced, the angle of repose of powders increased, bulk density and tap density decreased, and compressibility had a tendency of increase as flowability declined; The concentration of octyl acetate had an increased tendency and then decreased. While the concentration of octyl acetate in ultrafine powder prepared by optimal technology was the highest, the cumulative dissolution rate of AKBA in the ultramicro powder was higher than that in common powder. Conclusion: The application of optimal superfine pulverizing technique to Olibanum is feasible, and appropriate degree of micronization is helpful for the extraction and dissolution of effective components in Olibanum.

10.
Article in Chinese | WPRIM | ID: wpr-695938

ABSTRACT

This study was aimed to quickly identify Chinese medicine Olibanum.Thermal analysis method was used on the quality analysis of Chinese materia medica (CMM).A total of 25 batches of Olibanum on the market were collected.This study examined three important factors of temperature range,heating rate,powder mesh on the TGA and DTA thermal analysis experiments.And a method of rapid authentication of medicinal materials using TGA and DTA feature maps was built.Methods of the first-order points,connection on thermogravimetric analysis and heat enthalpy calculation were adopted in the quantitative analysis of Olibanum.The results showed that the best condition of TGA and DTA experiment on Olibanum was confirmed.The temperature range is 50-750℃.The heating rate is 20℃· min-1.The powder mesh is 100 mesh.Under these conditions,good quality goods of Olibanum,counterfeit Olibanum and adulterants of Olibanum could be distinguished through the characteristic peak (T1=447 ± 5℃,T2=549 ± 5℃,T3=350 ± 5℃),thermogravimetric analysis (TV-max,△W2+△W3) and thermal enthalpy analysis (△H).It was concluded that the TGA-DTA technology was simple.It was thought to be a rapid,accurate and simple new method for Olibanum identification and quality analysis.

11.
National Journal of Andrology ; (12): 157-163, 2017.
Article in Chinese | WPRIM | ID: wpr-812793

ABSTRACT

Objective@#To study the effects of muskolibanum combination on the proliferation and differentiation of prostate stem cells.@*METHODS@#We cultured prostate epithelial cells and urogenital sinus mesenchymal (UGSM) cells from 7-10 d old C57BL/6 mice and 16-18 d old pregnant C57BL/6 mice, transplanted the mixed suspension of the two types of cells under the kidney envelope of SCIDCB.17 male mice, and harvested the transplants 30 days later. We randomly divided the SCIDCB.17 mice into four groups to be treated intragastrically with musk (n = 8), olibanum (n = 8), musk+olibanum (n = 7), and normal saline (blank control, n = 8)) respectively, all for 14 days. Then we collected the kidney tissue for observation of the morphology of the glandular tubes and differentiation of different subsets of stem cells by HE staining and determination of the expressions and distribution of P63, CD133, CD117 and Sca1 by immunohistochemistry and Western blot.@*RESULTS@#A system was successfully established for the isolation and mixed culture of Sca1 Lin+ CD49f+ (LSC) cells of prostate stem cells and UGSM cells of the mouse embryonic prostate. Immunohistochemistry showed positive expressions of P63, CD133, Sca1, and CD117 in the prostatic acinar epithelia and proved the presence of prostatic acinar epithelial structure in the transplants. Compared with the blank control group, the expressions of CD133, Sca1 and CD117 were significantly increased in the musk, olibanum, and musk+olibanum groups (P< 0.05), higher in the musk+olibanum than in the musk or olibanum group (P< 0.05), and their protein expressions were even more elevated in the musk+olibanum group (P< 0.01), with statistically significant difference from the olibanum group (P< 0.05).@*CONCLUSIONS@#The combination of musk and olibanum can improve the proliferation and differentiation of prostate stem cells.


Subject(s)
Animals , Cell Differentiation , Cell Proliferation , Drug Therapy, Combination , Epithelial Cells , Cell Biology , Fatty Acids, Monounsaturated , Pharmacology , Female , Frankincense , Pharmacology , Male , Mesenchymal Stem Cells , Cell Biology , Mice , Mice, Inbred C57BL , Mice, SCID , Pregnancy , Prostate , Cell Biology , Random Allocation , Receptor Protein-Tyrosine Kinases , Receptors, Cholinergic , Stem Cells , Cell Biology
12.
Article in English | WPRIM | ID: wpr-812277

ABSTRACT

One of the leading causes of death worldwide is cardiovascular disease, hence searching for a cure is an important endeavor. The totally safe, edible, and inexpensive Boswellia plant exudate, known as olibanum or frankincense, is considered to possess diverse medicinal values in traditional medicine and from recent biological studies. Investigating the cardioprotective and antioxidant activities of olibanum from a Boswellia species, family Bursearaceae, namely Boswellia carteri Birdw. was the aim of this study. Cardioprotective activity was evaluated using a model of myocardial infarction induced by isoprenaline (ISO), while antioxidant activity was tested adopting nitric oxide scavenging (NOS) and azino-bis-3-ethyl benzthiazoline-6-sulfonic acid (ABTS) assays. The results revealed a mild cardioprotective effect and weak antioxidant activity.


Subject(s)
Animals , Antioxidants , Boswellia , Chemistry , Frankincense , Humans , Male , Myocardial Infarction , Drug Therapy , Pathology , Myocardium , Pathology , Rats , Rats, Wistar
13.
Article in Chinese | WPRIM | ID: wpr-461675

ABSTRACT

This study was aimed to establish a method for the determination of octylacetate in Jing-Fu-Kang (JFK) granules. The content was determined by GC with Agilent HP-5 (30 m × 0.32 mm × 0.25μm) capillary column, with hydrogen flame ionization (FID) as detector. The injector temperature was 220℃. The detector temperature was 260℃. The split ratio was 20:1. At first, the temperature should be maintained to 80℃for 2 min, and then heating up to 200℃with the speed of 10℃·min-1. This temperature was maintained for 10 min. The results showed that the good linear relationship of octylacetate was within the range of 0.156 5-7.824 2 mg·mL-1, r=0.999 9. The average recovery rate was 100.00%, and RSD was 1.91%(n=9). The lowest detection limit of octylacetate was 1.633 6 ng. It was concluded that the established method was simple and reproducible with no interference. It can be applied to the quality control of JFK granules.

14.
Chinese Pharmaceutical Journal ; (24): 989-992, 2012.
Article in Chinese | WPRIM | ID: wpr-860708

ABSTRACT

OBJECTIVE: To improve the quality standards of Huoxue Zhitong Powders and Huoxue Zhitong Capsules. METHODS: Angelicae Sinensis Radix, Olibanum and Borneolum Syntheticum were identified simultaneously by TLC method. Ferulic acid was detected by HPLC method. RESULTS: Clear spots were obtained with good separation in TLC identification. For the HPLC determination of ferulic acid, the calibration curve was linear in the range of 0.2-200 μg · mL-1, the limit of detection was 0.054 μg · mL-1 and recoveries were between 95%-105%. CONCLUSION: The proposed method is specific, accurate, simple and applicable for better control of Huoxue Zhitong Preparations' quality.

15.
Article in English | WPRIM | ID: wpr-728747

ABSTRACT

Olibanum (Boswellia serrata) has been shown to have anti-inflammatory, anti-arthritic and anti- cancer effects. This study determined the role of a water extract of olibanum in platelet-derived growth factor (PDGF)-stimulated proliferation and migration of rat aortic smooth muscle cells (RASMCs). PDGF-BB induced the migration and proliferation of RASMCs that were inhibited by olibanum extract in a dose-dependent manner. The PDGF-BB-increased phosphorylation of p38 mitogen-activated protein kinase (MAPK); the heat shock protein (Hsp) 27 was significantly inhibited by the olibanum extract. The effects of PDGF-BB-induced extracellular signal-regulated kinase1/2 was not altered by the olibanum extract. Treatment with olibanum extract inhibited PDGF-BB-stimulated sprout out growth of aortic rings. These results suggest that the water extract of olibanum inhibits PDGF-BB-stimulated migration and proliferation in RASMCs as well as sprout out growth, which may be mediated by the inhibition of the p38 MAPK and Hsp27 pathways.


Subject(s)
Animals , Boswellia , Cell Movement , Heat-Shock Proteins , Muscle, Smooth, Vascular , Myocytes, Smooth Muscle , p38 Mitogen-Activated Protein Kinases , Phosphorylation , Platelet-Derived Growth Factor , Protein Kinases , Proto-Oncogene Proteins c-sis , Rats , Water
16.
China Pharmacy ; (12)2007.
Article in Chinese | WPRIM | ID: wpr-529684

ABSTRACT

OBJECTIVE:To study the supercritical CO2 fluid extraction(SFE-CO2)technology of Olibanum.METHODS:The optimum extraction technology was obtained through orthogonal test with the content of acetic octylester determined by GC and taken as index for the evaluation of the technology.RESULTS:The optimal conditions were as follows:pressure 25 MPa,temperature 60℃ and extraction time 3h.CONCLUSION:The process of SFE-CO2 is suitable for Olibanum extraction.

17.
China Pharmacy ; (12)2001.
Article in Chinese | WPRIM | ID: wpr-529097

ABSTRACT

OBJECTIVE: To study the optimum technique for extraction of mixture volatile oil from Olibanam and Sandalwood. METHODS: The orthogonal test was adopted to optimize the extraction process using the yield rate of volatile oil as indicator. RESULTS: The optimum extraction conditions were as follows: powder forming by 10mesh, 10- fold water added, soaking for 10h and extraction for 8h. CONCLUSIONS: This extraction method is simple, efficient and suitable for industria-lized production.

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