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1.
Article in Chinese | WPRIM | ID: wpr-846293

ABSTRACT

Objective: To establish a method for the simultaneous content determination of 14 chemical components such as D- amygdalin, puerarin, hesperidin in Fenghan Ganmao Granules (FGG) by UHPLC-UV wavelength switching method, and chemometric analysis was used to analyze the quality differences. Methods: Separation was performed on an Agilent Poroshell 120 EC-C18 (150 mm × 2.1 mm, 2.7 μm) with a gradient elution of acetonitrile and 0.1% phosphoric acid. The content of 14 chemical components in 68 batches of samples from five manufacturers was determined by switching wavelength (210, 254, 310 nm). The radar chart, similarity evaluation, heat map and hierarchical clustering analysis, and principal component analysis (PCA) were used for data analysis. Results: The content of each component was as follows, D-amygdalin 0.063-3.885 mg/g, 3'-hydroxy puerarin 0.012-1.540 mg/g, puerarin 0.036-4.017 mg/g, 3'-methoxy puerarin 0.016-1.837 mg/g, puerarin-6″-O-xyloside 0.004-0.449 mg/g, mirificin 0.021-2.076 mg/g, daidzin 0.010-1.527 mg/g, prim-O-glucosylcimifugin 0.007-0.471 mg/g, 5-O-methylvisammioside 0.062-1.029 mg/g, hesperidin 0.210-8.453 mg/g, rosmarinic acid 0.001-0.237 mg/g, oxypeucedanin hydrate 0.007-0.204 mg/g, glycyrrhizic acid 0.056-1.311 mg/g, oxypeucedanin 0.002-0.042 mg/g, respectively. Chemometric analysis showed that there were some differences among the samples from different manufacturers, and the samples from the same manufacturer were more consistent. Conclusion: The method is simple, reproducible, and specific, which provides a reference method for the overall quality evaluation of FGG.

2.
Article in Chinese | WPRIM | ID: wpr-846044

ABSTRACT

Objective: To establish the fingerprint of Jinzhen Oral Liquid (JOL) by HPLC-UVD-ELSD and determine the main 13 representative components (gallic acid, liquiritin, aloe-emodin-8-O-β-D-glucopyranoside, liquiritigenin, baicalin, chrysin-7-O-β-D- glucoronide, oroxyloside, wogonoside, chrysophal-1-O-β-D-glucopyranoside, chrysophal-8-O-β-D-glucopyranoside, rhein, glycyrrhizic acid, hyodeoxycholic acid and cholic acid) simultaneously, in order to provide reference for the overall quality control of JOL. Methods: The separation was developed on Cosmosil-C18 column (250 mm × 4.6 mm, 5 μm) by gradient elution with methanol-water [containing 0.1% formic acid] at 254 nm, the temperature of drift tube was maintained at 115 ℃ and the carrier gas flow rate was 2.0 L/min. An HPLC-UVD-ELSD fingerprint of JOL was set up, and 15 batches of JOL were evaluated by similarity assay. Furthermore, the contents of the main 13 representative components were determined on the premise of small disparities among batches. Results: The HPLC-UVD-ELSD fingerprint of JOL was established with good separation, and 13 chemical components were determined simultaneously. Fifteen main characteristic peaks [gallic acid (peak 1), liquiritin (peak 5), aloe-emodin-8-O-β-D-glucopyranoside (peak 7), liquiritigenin (peak 11), baicalin (peak 13), chrysin-7-O-β-D-glucoronide (peak 16), oroxyloside (peak 17), wogonoside (peak 18), chrysophal-8-O-β-D-glucopyranoside (peak 19), chrysophal-1-O-β-D-glucopyranoside (peak 20), rhein (peak 24), glycyrrhizic acid (peak 26), (18β,20α)-glycyrrhizic acid (peak 27), hyodeoxycholic acid (peak 28), cholic acid (peak 29)] from four formula of JOL were chemically identified and 29 main characteristic peaks were assigned to individual herbs (peaks 8, 12, 13, 15-18 originate from Scutellariae Radix, peaks 3-5, 10, 11, 25-27 originate from Glycyrrhizae Radix et Rhizoma, peaks 1, 6, 7, 9, 14, 19, 20, 24 originate from Rhei Radix et Rhizoma, peak 2 originates from Fritillariae Ussuriensis Bulbus, peaks 28, 29 originate from Bovis Calculus Artifactus, peaks 21-23 originate from auxiliary materials). The similarity of 15 batches of JOL was about 0.968 to 1.000. Moreover, good linear relationships were found (R2=0.999 0-0.999 9), and the average recovery rates were 96.90%-102.84%. The content range of quantitative components in 15 batches of JOL (gallic acid 51.82-148.27 μg/mL, liquiritin 75.04-130.00 μg/mL, aloe-emodin-8-O-β-D-glucopyranoside 31.72-39.84 μg/mL, liquiritigenin 14.24-43.65 μg/mL, baicalin 610.37-867.40 μg/mL, chrysin-7-O-β-D-glucoronide 12.87-34.09 μg/mL, oroxyloside 62.45-101.48 μg/mL, wogonoside 155.41-205.86 μg/mL, chrysophal-1-O-β-D-glucopyranoside 11.56-23.72 μg/mL, chrysophal-8-O-β-D- glucopyranoside 16.14-36.87 μg/mL, glycyrrhizic acid 222.97-310.32 μg/mL, hyodeoxycholic acid 177.48-239.70 μg/mL, cholic acid 98.54-132.85 μg/mL) was determinated. Conclusion: The qualitative and quantitative methods of HPLC-UVD-ELSD mentioned above provided important evidence for further improving the overall quality standard of JOL.

3.
Chinese Pharmaceutical Journal ; (24): 1504-1510, 2018.
Article in Chinese | WPRIM | ID: wpr-858230

ABSTRACT

OBJECTIVE: To establish the fingerprint of Xianling Gubao Capsules (XLGB) by multi-wavelength HPLC-UVD-ELSD and determine the main representative components simultaneously, in order to provide reference for the overall quality control of XLGB.METHODS: The separation was developed on Poroshell 120 EC-C18 column (4.6 mm×100 mm, 2.7 μm) by gradient elution with methanol-water at 240, 270 and 334 nm, the temperature of drift tube was maintained at 105 ℃ and the carrier gas flow rate was 2.0 L•min-1. An HPLC-UVD-ELSD fingerprint of XLGB was set up, and 15 batches of XLGB were evaluated by similarity assay. Furthermore, the contents of the main representative components were determined on the premise of small disparities between batches. RESULTS: The multi-wavelength HPLC-UVD-ELSD fingerprint of XLGB was established with good separation, and six chemical components were determined simultaneously. Thirty-one main characteristic peaks from six herbs of XLGB were chemically identified and 26 main characteristic peaks were assigned to individual herbs. The similarity of 15 batches of XLGB was about 0.943 to 0.997. Moreover, good linear relationships were found (r=0.999 0-0.999 6), and the average recovery rates were 98.5%-104.9%. CONCLUSION: The method can be used for the overall quality control of XLGB with good specificity and high efficiency and is close to the actual production, which is promising to be further improved to be used as an industry internal control standard.

4.
Chinese Hospital Management ; (12): 73-75, 2018.
Article in Chinese | WPRIM | ID: wpr-706626

ABSTRACT

The quality control centers of the city level supervise and manage the medical quality of related clinical and technical specialties of medical institutions.The paper researches the comprehensive control and management of various professional subjects according to the standard of quality control,improving the medical quality of the Department,improving the level of discipline development,promoting the continuous improvement of medical quality in hospitals.

5.
Article in English | WPRIM | ID: wpr-812547

ABSTRACT

Boiling processing is commonly used in post-harvest handling of White Paeony Root (WPR), in order to whiten the herbal materials and preserve the bright color, since such WPR is empirically considered to possess a higher quality. The present study was designed to investigate whether and how the boiling processing affects overall quality of WPR. First, an ultra-high performance liquid chromatography quadrupole/time-of-flight mass spectrometry-based metabolomics approach coupled with multivariate statistical analysis was developed to compare the holistic quality of boiled and un-boiled WPR samples. Second, ten major components in WPR samples boiled for different durations were quantitatively determined using high performance liquid chromatography to further explore the effects of boiling time on the holistic quality of WPR, meanwhile the appearance of the processed herbal materials was observed. The results suggested that the boiling processing conspicuously affected the holistic quality of WPR by simultaneously and inconsistently altering the chemical compositions and that short-time boiling processing between 2 and 10 min could both make the WPR bright-colored and improve the contents of major bioactive components, which were not achieved either without boiling or with prolonged boiling. In conclusion, short-term boiling (2-10 min) is recommended for post-harvest handling of WPR.


Subject(s)
Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Chemistry , Reference Standards , Hot Temperature , Mass Spectrometry , Methods , Paeonia , Chemistry , Plant Roots , Chemistry , Technology, Pharmaceutical , Water
6.
Article in Chinese | WPRIM | ID: wpr-467084

ABSTRACT

In order to improve students' overall quality,Biomedical Engineering College of Chongqing Medical University has carried out quality development training in the 6 session of the 450 students.The content includes development projects,the queue training,humanities lectures,inspirational film etc.; the objective is to develop the students' mind,develop their team spirit and interpersonal communication skills.College has recruited and assisted professional instructors in the whole training.The centralized training in specific situations gives students a strong spiritual shock and strong positive energy.Before and after the training we use the same questionnaire to students,the results of which have shown that their comprehensive ability,EQ,and cohesion of the class have been improved.

7.
Article in Chinese | WPRIM | ID: wpr-427735

ABSTRACT

We changed the form of literature reading from simple reading and translation to comprehensive lecture,analysis,discussion and exchange under the guidance of teachers in order to promote postgraduates' capability of learning professional English and to enhance their overall quality.The new form not only improves students' English proficiency but also enhances their overall quality,such as literature quality,teaching ability,competitive consciousness,psychological quality,etc.At the same time,it is also beneficial for teachers.

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