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1.
Article in English | WPRIM | ID: wpr-929253

ABSTRACT

Two new neolignans and one new lignan (1-3) were obtained from the roots of Paeonia lactiflora. Their structures were unambiguously elucidated based on extensive spectroscopic analysis, single-crystal X-ray crystallography, and the calculated and experimental electronic circular dichroism (ECD) spectra. Compound 1 was a racemic mixture and successfully resolved into the anticipated enantiomers via chiral-phase HPLC. Compound 3 demonstrated moderate inhibitory activity against human carboxylesterase 2A1 (hCES2A1) with an IC50 value of 7.28 ± 0.94 μmol·-1.


Subject(s)
Chromatography, High Pressure Liquid , Humans , Lignans/chemistry , Paeonia , Plant Roots/chemistry , Stereoisomerism
2.
Article in Chinese | WPRIM | ID: wpr-927979

ABSTRACT

Paeonia lactiflora is an important medicinal resource in China. It is of great significance for the protection and cultivation of P. lactiflora resources to find the suitable habitats. The study was based on the information of 98 distribution sites and the data of 20 current environmental factors of wild P. lactiflora in China. According to the correlation and importance of environmental factors, we selected the main environmental factors affecting the potential suitable habitats. Then, BCC-CSM2-MR model was employed to predict the distribution range and center change of potential suitable habitat of wild P. lactiflora in the climate scenarios of SSP1-2.6, SSP2-4.5, SSP3-7.0, and SSP5-8.5 during 2021-2100. The ensemble model combined with GBM, GLM, MaxEnt, and RF showed improved prediction accuracy, with TSS=0.85 and AUC=0.95. Among the 20 environmental factors, annual mean temperature, monthly mean diurnal range of temperature, temperature seasonality, mean temperature of the warmest quarter, precipitation of the wettest month, precipitation seasonality, precipitation of the driest quarter, and elevation were the main factors that affected the suitable habitat distribution of P. lactiflora. At present, the potential suitable habitats of wild P. lactiflora is mainly distributed in Inner Mongolia, Heilongjiang, Jilin, Liaoning, Hebei, Beijing, Shaanxi, Shanxi, Shandong, Gansu, Xinjiang, Tibet, and Ningxia, and concentrated in the northeastern Inner Mongolia, central Heilongjiang, and northern Jilin. Under future climate conditions, the highly sui-table area of wild P. lactiflora will shrink, and the potential suitable habitat will mainly be lost to different degrees. However, in the SSP5-8.5 scenario, the low suitable area of wild P. lactiflora will partially increase in the highlands and mountains in western China including Xinjiang, Tibet, and Qinghai during 2061-2100. The distribution center of wild P. lactiflora migrated first to the northeast and then to the southwest. The total suitable habitats were stable and kept in the high-latitude zones. The prediction of the potential geo-graphical distribution of P. lactiflora is of great significance to the habitat protection and standardized cultivation of this plant in the future.


Subject(s)
China , Climate , Climate Change , Ecosystem , Paeonia
3.
China Pharmacy ; (12): 2613-2618, 2021.
Article in Chinese | WPRIM | ID: wpr-904519

ABSTRACT

OBJECTIVE:To optimize the processing technology of Paeonia lactiflora stir-baked with wine ,and to investigate its in vitro anticoagulant effect. METHODS :The weight coefficient of each index was determined and the comprehensive score was calculated with analytic hierarchy process (AHP),Criteria Importance Though Intercrieria Correlation (CRITIC)and AHP-CRITIC weighting method ,using the contents of oxypaeoniflorin ,albiflorin,paeoniflorin,benzoic acid and water-soluble extract as index. Box-Behnken response surface methodology was used to optimize the parameters of P. lactiflora stir-baked with wine ,such as moistening time ,frying time and frying temperature. Then in vitro anticoagulant experiment was used to investigate the pharmacodynamics of P. lactiflora stir-baked with wine prepared according to the optimal processing technology. RESULTS :The weight coefficients of albiflorin ,oxypaeoniflorin,paeoniflorin,benzoic acid and water-soluble extract determined by AHP-CRITIC weighting method were 0.233 9,0.131 7,0.183 3,0.078 9,0.372 3,respectively;the optimal processing technology of P. lactiflora stir-baked with wine included moistening time of 35 min,frying time of 20 min and frying temperature of 120 ℃. The results of in vitro anticoagulant test showed that P. lactiflora and P. lactiflora stir-baked with wine could significantly prolong the time of thrombin time ,prothrombin time ,activated partial thrombin time of plasma ;the effects of P. lactiflora stir-baked with wine were significantly better than those of P. lactiflora (P<0.05). CONCLUSIONS :The optimized processing technology of P. lactiflora stir-baked with wine is stable and feasible. The in vitro anticoagulant effect of P. lactiflora stir-baked with wine prepared by this tehcnology is better than that of raw products.

4.
Article in English | WPRIM | ID: wpr-922765

ABSTRACT

Guided by cell-based anti-anaphylactic assay, eighteen cage-like monoterpenoid glycosides (1-18) were obtained from the bioactive fraction of P. lactiflora extract. Among these, compounds 1, 5, 6, 11, 12, 15, and 17 significantly reduced the release rate of β-HEX and HIS without or with less cytotoxicity. Furthermore, the most potent inhibitor benzoylpaeoniflorin (5) was selected as the prioritized compound for the study of action of mechanism, and its anti-anaphylactic activity was medicated by dual-inhibiting HDC and MAPK signal pathway. Moreover, molecular docking simulation explained that benzoylpaeoniflorin (5) blocked the conversion of L-histidine to HIS by occupying the HDC active site. Finally, in vivo on PCA using BALB/c mice, benzoylpaeoniflorin (5) suppressed the IgE-mediated PCA reaction in antigen-challenged mice. These findings indicated that cage-like monoterpenoid glycosides, especially benzoylpaeoniflorin (5), mainly contribute to the anti-anaphylactic activity of P. lactiflora by dual-inhibiting HDC and MAPK signal pathway. Therefore, benzoylpaeoniflorin (5) may be considered as a novel drug candidate for the treatment of anaphylactic diseases.


Subject(s)
Animals , Glucosides , Mice , Mice, Inbred BALB C , Molecular Docking Simulation , Monoterpenes , Paeonia , Plant Roots
5.
Article in Chinese | WPRIM | ID: wpr-887957

ABSTRACT

A new phenolic acid ester, 4'-hydroxyphenylethyl 4,8(R)-dihydroxyphenylpropionate(1), was isolated from an endophytic fungus Colletotrichum capsici of Paeonia lactiflora roots, along with eight known phenolic derivatives, tyrosol(2), 2-(4-hydroxyphenyl) ethyl acetate(3), methyl p-hydroxyphenylacetate(4), methyl m-hydroxyphenylacetate(5), 4-(4-hydroxyphene-thoxy)-4-oxobutanoic acid(6), 4-hydroxyphenethyl methyl succinate(7), trichodenol B(8) and 4-hydroxyphenethyl 2-(4-hydroxyphenyl) acetate(9). Their structures were identified by a combination of high-resolution electrospray ionization mass spectrometry(HR-ESI-MS), nuclear magnetic resonance(NMR) spectroscopy, ultraviolet(UV) spectroscopy and electronic circular dichroism(ECD) spectroscopy. Compounds 2-9 were isolated from this fungus for the first time.


Subject(s)
Colletotrichum , Esters , Hydroxybenzoates , Paeonia
6.
Article in Chinese | WPRIM | ID: wpr-878890

ABSTRACT

According to human carboxylesterase 2(hCE2) inhibitors reported in the literature, the pharmacophore model of hCE2 inhibitors was developed using HipHop module in Discovery Studio 2016. The optimized pharmacophore model, which was validated by test set, contained two hydrophobic, one hydrogen bond acceptor, and one aromatic ring features. Using the pharmacophore model established, 5 potential hCE2 inhibitors(CS-1,CS-2,CS-3,CS-6 and CS-8) were screened from 20 compounds isolated from the roots of Paeonia lactiflora, which were further confirmed in vitro, with the IC_(50) values of 5.04, 5.21, 5.95, 6.64 and 7.94 μmol·L~(-1), respectively. The results demonstrated that the pharmacophore model exerted excellent forecasting ability with high precision, which could be applied to screen novel hCE2 inhibitors from Chinese medicinal materials.


Subject(s)
Carboxylesterase/metabolism , Humans , Hydrogen Bonding , Hydrophobic and Hydrophilic Interactions
7.
Article in Chinese | WPRIM | ID: wpr-872998

ABSTRACT

Objective::To investigate the chemical constituents from Paeonia veitchii. Method::P. veitchii samples (30 kg) were extracted with 95% ethanol for four times and then filtrated, and the combined filtrates were concentrated under vacuum to get the extracts. After suspension with water, exaction was conducted with petroleum ether, dichloromethane, ethyl acetate, n-butanol and water successively to obtain five corresponding fractions. The compounds were isolated and purified by silica gel, Sephadex LH-20, ODS column chromatography and prep-HPLC, and the structures of these compounds were determined by such spectrum technologies as infrared spectroscopy (IR), mass spectroscopy (MS), and nuclear magnetic resonance (NMR). Result::Sixteen compounds were isolated and their structures were identified as follows (1S, 5R, 6R)-1, 8-dihydroxypin-2-en-4-one (1), (2-hydroxyl)-phenyl-methyl-β-D-xylopyranoside (2), flufuran (3), 6′-O-vanillylpaeoniflorin (4), methyl 2, 5-dihydroxycinnamate (5), (1S, 2S, 5R, 6R)-1, 8-dihydroxypin-4-one (6), palbinone (7), 4-O-methylpaeoniflorin (8), 4-O-ethylpaeoniflorin (9), benzoyloxypaeoniflorin (10), benzoic acid (11), gallic acid (12), methyl gallate (13), ethyl gallate (14), β-sitosterol (15), and 1, 2, 3, 4, 6-penta-O-galloyl-β-D-glucopyranose (16). Conclusion::Compounds 1, 2 were new natural compounds; compound 3 was isolated from genus Paeonia for the first time, and compounds 4-7 were isolated from this plant for the first time.

8.
China Pharmacy ; (12): 1595-1600, 2020.
Article in Chinese | WPRIM | ID: wpr-822625

ABSTRACT

OBJECTIVE:To study the improvement effects of total glucosides of Paeonia l actiflora(TGPL)combined with cisplatin on tumor inhibition and renal injury in gastric cancer model rats. METHODS :Totally 75 rats were divided into control group(normal saline ,i.g.),model group (normal saline ,i.g.),cisplatin group (25 mg/kg,i.p.),TGPL group (200 mg/kg,i.g.), TGPL+cisplatin group (200 mg/kg TGPL ,i.g.+25 mg/kg cisplatin ,i.p.),with 15 rats in each group. Except for control group , gastric cancer rat models were established in other groups by subcutaneous inoculation of gastric cancer BGC 823 cell suspension. After modeling ,they were given relevant medicine intraperitoneally/intragastrically ,for consecutive 28 d. The tumor weight and tumor inhibition rate were measured. The apoptosis rate in tumor tissue was detected by flow cytometry ;Western blotting assay was used to detect the levels of Bcl- 2 and Bax in tumor tissue ;ELISA or biochemical analyzer were used to determine the levels of IL-18 and KIM- 1 in urine ,BUN and Scr in serum ,as well as SOD, MDA and GSH in renal tissue ; histopathological 015)changes of renal tissue in rats were detected by HE staining. 65896961。E-mail:taolitianxia999@163.com RESULTS: Compared with control group , there was no statistical significance in the levels of IL- 18 and KIM- 1 in urine,serum levels of BUN and Scr ,the levels of SOD MDA and GSH in renal tissue of model group (P>0.05);no obvious histopathological change was found in renal tissue. Compared with model group ,tumor weight in cisplatin group was decreased significantly ,while cell apoptosis was increased significantly,while expression level of Bax in tumor tissue was increased significantly ,the expression level of Bcl- 2 was decreased significantly(P<0.05);the levels of IL- 18 and KIM- 1 in urine ,BUN and Scr in serum and MDA in kidney were increased significantly(P<0.05);the levels of SOD and GSH in renal tissue were decreased significantly (P<0.05);renal tubules were dilated,vacuoles of epithelial cells in basal layer and cast in renal tubules formed . Compared with cisplatin ,tumor weight in TGPL+cisplatin group was decreased significantly ,while apoptotic rate was increased significantly ,while the level of Bax was increased significantly ,expression level of Bcl- 2 was decreased significantly (P<0.05);the levels of IL- 18 and KIM- 1 in urine , BUN and Scr in serum and MDA in renal tissue were significantly reduced (P<0.05);the levels of SOD and GSH in renal tissue were increased significantly (P<0.05);there was no tubule dilation ,but vacuoles were occasionally found in basal epithelial cells,and the cast formation in renal tubules was rare. Compared with TGPL group,tumor weight in TGPL +cisplatin group was significantly reduced ,the tumor inhibition rate was significantly increased ,the apoptosis rate was significantly increased ,while the expression level of Bax in tumor tissue was increased significantly ,expression level of Bcl- 2 was decreased significantly (P< 0.05);there was no statistical significance in the levels of IL- 18 and KIM- 1 in urine ,the levels of BUN and Scr in serum ,the levels of SOD ,MDA and GSH in renal tissue (P>0.05);a small amount of inflammatory cells infiltrated occasionally in renal tissue. CONCLUSIONS :TGPL combined with cisplatin can inhibit tumor growth ,promote tumor cell apoptosis and improve renal injury induced by cisplatin.

9.
Article in English | WPRIM | ID: wpr-881032

ABSTRACT

The depressant-like effects of albiflorin (AF) were studied on stressed chronic restraint stress (CRS) rats. Experimental rats were subjected to immobilization stress for a daily 6 h-restraining in a plastic restrainer for continuous 21 d and were treated with 30 or 15 mg·kg

10.
Article in Chinese | WPRIM | ID: wpr-846117

ABSTRACT

Objective: Through network pharmacology, the network relationship between the active component of Sanqi Mixture, the target of hepatic ischemia- reperfusion injury(HIRI), and biological pathway was constructed to explore the key target and mechanism of effect of Sanqi Mixture on HIRI. Method: Through literature research at home and abroad, Traditional Chinese Medicine Systems Pharmacology (TCMSP) platform, Pharm Mapper, Swiss Target Prediction and other servers, oral availability (OB) and drug-likeness (DL) were selected as the limited conditions to collect the relevant targets for Sanqi Mixture for intervention in HIRI. The OMIM database was used to screen and collate HIRI related genes and protein targets. Excel table was used to merge and sort the intersection between disease and targets through Cytoscape3.7.2 software plug-ins Network Analyzer, with topological parameters (degree) ≥ 5 (average degrees of freedom 4.5) for the filter to find the core targets; And the intersection targets were imported to the server STRING, and with Confidence Score of 0.85 or higher for the filter conditions to build the core protein interactions (Hub-PPI) network. The intersection target was introduced into FunRich 3.0 software for biological process and biological pathway analysis, and Cytoscape3.7.2 was used to construct the network of "traditional Chinese medicine-active ingredient-HIRI target-biological pathway". Result: Sanqi mixture could reduce the expression of Aspartate aminotransferase (AST) and glutamate transaminase (ALT) in HIRI mice (P < 0.01). After screening, 45 active components of Sanqi Mixture were obtained, corresponding to 3 273 targets, and the main compounds included ursolic acid, oleanolic acid, brucine, quercetin, ginsenoside F2, paeoniflorin, etc. Among the 196 targets obtained by HIRI, 46 targets were intersected with components, including 11-β-hydroxysteroid dehydrogenase (HSD11B1), adenosine receptor A3 (ADORA3), cyclooxygenase 2 (PTGS2), adenosine receptor A1 (ADORA1), protein kinase C-ε (PKC), etc. With the STRING server setting the qualified condition of Confidence Score ≥ 0.85, the PPI network with high Confidence was obtained and clustered into three categories through cluster processing. Five biological processes including protein metabolism, signal transduction, negative regulation of enzyme activity, inflammatory response and transmembrane receptor protein tyrosine kinase signal pathway were analyzed by FunRich software (P < 0.05). 16 biological pathways including integrin-linked kinase signal, TNF receptor signaling pathway, P38 mitogen-activated protein kinase signaling pathway, and TRAIL signaling pathway (P < 0.01). Conclusion: It is preliminarily discussed that Sanqi Mixture intervenes HIRI through the interaction of multiple components and multiple targets, as well as the regulation of multiple biological pathways and biological processes. However, the key core targets and the specific regulation mechanism still need further experimental verification.

11.
Article in Chinese | WPRIM | ID: wpr-846008

ABSTRACT

Objective: To study the constituents from the roots of Paeonia lactiflora. Methods: The chemical constituents were isolated by various chromatographic techniques and their structures were elucidated by physicochemical properties and NMR data. Results: Eleven compounds were obtained and characterized as (4S)-perillic acid 6-O-α-L-arabinopyranosyl-(1'→6'')- β-D-glucopyranosyl (1), 4,9-dihydroxy-8,10-dehydrothymol-1-O-β-D-glucoside (2), emodin-8-O-β-D-glucoside (3), resveratrol (4), β-D-glucopyranosyl benzoate (5), ilexperphenoside A (6), catechol (7), methyl gallate (8), ethyl gallate (9), 3-methoxygallic acid (10), 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranoside (11). Conclusion: Compound 1 is identified as a new compound, named perillic acid glycoside, compounds 2 and 5 are identified from the genus Paeonia for the first time.

12.
Article in Chinese | WPRIM | ID: wpr-828069

ABSTRACT

A new lignan glucoside,(+)-fragransin A_2-4-O-β-D-glucopyranoside(1), has been isolated from the dry root of Paeonia lactiflora by column chromatography on silica gel, Sephadex LH-20, and MCI-gel resin, as well as preparative RP-HPLC. The structure of the new compound was elucidated by spectroscopic data analysis(MS, UV, IR, CD, 1 D and 2 D NMR) and chemical method. Compound 1 showed moderate inhibition against lipopolysaccharide induced nitric oxide production in RAW264.7 macrophages, with an IC_(50) value of 21.3 μmol·L~(-1).


Subject(s)
Chromatography, High Pressure Liquid , Glucosides , Lignans , Paeonia , Plant Extracts
13.
China Pharmacy ; (12): 441-446, 2020.
Article in Chinese | WPRIM | ID: wpr-817289

ABSTRACT

OBJECTIVE:To establish the method for content determination of 6 active ingredients in Paeonia lactiflora during different harvest periods ,and to investigate its variation rules so as to determine the optimal harvesting period. METHODS :HPLC method was adopted to determine the contents of gallic acid , catechin, alibiflorin, paeoniflorin, benzoic acid and benzoylpaeoniflorin,and principal component analysis was conducted . The determination was performed on Thermo C 18 column with mobile phase consisted of acetonitrile- 0.1% phosphoric acid aqueous solution (gradient elution )at the flow rate of 1.0 mL/min. The detection wavelength was 230 nm,and column temperature was 35 ℃. The sample size was 10 µL. RESULTS :The linear range of the above 6 ingredients were 0.013 2-0.25 mg/mL(r=0.999 2),0.013 2-0.25 mg/mL(r=0.999 9),0.026 8-0.51 mg/mL(r=0.999 7), 0.42-8.01 mg/mL(r=0.999 2),0.016-0.31 mg/mL(r=0.999 4),0.02-0.38 mg/mL(r=0.999 8),respectively. The limits of quantification were 0.009 3,0.008 5,0.016 3,0.021 7,0.011 3,0.017 4 mg/mL,and the limits of detection were 0.003 3,0.002 7, 0.005 4,0.007 3,0.003 8,0.005 9 mg/mL. RSDs of precision ,stability and repeatability tests were less than 2%. The recoveries were 96.01%-99.43%(RSD=1.23%,n=9),97.95%-100.45%(RSD=0.79%,n=9),97.98%-100.11%(RSD=0.68%,n= 9),98.83% -100.09% (RSD=0.65% ,n=9),98.58% - 100.95%(RSD=1.35%,n=9),96.28%-103.26%(RSD= 1.76%,n=9). The contents of above 6 ingredients in Radix Paeoniae Rubra (roots) were 0.016% -0.057% ,0-0.067% , 0.207%-0.640%,2.350%-5.887%,0.030%- 0.245%,0.054%- 0.381%,respectively. On May 30th,the drying rate of Radix 0451-87266873。E-mail:wangzhen_yue@163.com Paeoniae Rubra was the lowest (about 33%),and on Sept. 15th,the drying rate was the highest (about 49%). The contents of gallic acid and paeoniflorin in the leaves of P. lactiflora were higher than the root during Jul.-Oct. Results of principal component analysis showed that the variance contribution rates of the first two principal components were 71.845% and 18.170%,respectively;cumulative variance contribution rate was 90.015%. The months with higher comprehensive scores were May to Jun. and Sept. to Oct. CONCLUSIONS :Established method is simple , accurate,reproducible and precise. It can be used to determine the contents of 6 active ingredients in Radix Paeoniae Rubra during different harvest periods. Sept. 30th to Oct. 15th is the optimum harvesting periods for Radix Paeoniae Rubra ,and leaves can be harvested around Jul. 15th.

14.
Acta Pharmaceutica Sinica ; (12): 168-176, 2020.
Article in Chinese | WPRIM | ID: wpr-780571

ABSTRACT

The whole chloroplast genome ofthe medicinal plant Paeonia mairei H. Lév. was sequenced using the Illumina HiSeq X Ten platform and then assembled, annotated, and characterized by bioinformatic methods in this study. The complete chloroplast genome of P. mairei is 152 731 bp in length with the typical quadripartite structure, which consists of a large single copy-region (LSC, 84 402 bp), a small single copy-region (SSC, 16 969 bp), and a pair of inverted repeat regions (IRa and IRb, 25 680 bp), with an overall GC content of 38.4%. A total of 136 predicted genes, including 90 protein-coding genes, 38 tRNA genes and eight rRNA genes were identified. Among these, seven protein-coding genes, seven tRNA genes and four rRNA genes were found duplicated in the IR regions. In addition, 28 dispersed repeats, 10 tandem repeats, and 64 simple sequence repeats were detected within the whole chloroplast genome of P. mairei. Comparative analyses between 12 Peaonia species showed that the chloroplast genomes are highly conserved in length, gene content, gene order, and GC content. Meanwhile, the noncoding sequences (intergenic regions and introns) show a higher variation than the protein coding sequences, and sequences from the LSC region and SSC region are more variable than those from the IR regions. P. mairei was inferred forming in a distinct clade with P. lactiflora, P. obovate, and P. anomala subsp. veitchii with a 100% bootstrap value and is phylogenetically closest to P. lactiflora. These results may provide a basis for further genetic studies and the development and utilization of medicinal P. mairei.

15.
Clinical Medicine of China ; (12): 451-456, 2019.
Article in Chinese | WPRIM | ID: wpr-754334

ABSTRACT

Objective To investigate the efficacy of total glucosides of paeony ( TGP ) in the treatment of hematuria and proteinuria purpura nephritis ( HSPN ) and serum interleukin?6 ( IL?6 ), interleukin?1 beta (IL?1 beta) and interleukin?18 (IL?18).Methods From June 2017 to December 2018, 64 cases of children with primary hematuria and proteinuria purpura nephritis admitted to the department of pediatrics affiliated hospital of Xuzhou medical university were selected as study subjects,and were divided into control group (33 cases) and observation group (31 cases) according to random number method.The children in the control group were given oral administration of poniasone acetate,and the observation group was treated with TGP on the basis of the control group.Both groups received continuous treatment for 4 weeks.Two groups′ before and after treatment expression level of serum interleukin?6, interleukin?1β and interleukin?18,and level of clinic indicator such as Urinary red biood cell (URBC),24 hour urinary protein quantity, urinary immune globulin G ( IGU ), micro?albuminuria ( urinary microalbumin, MAU ), α?Microglobulin (α1?MG) and urinary transferrin ( TFRU) is made a comparison.Results After 4weeks of treatment,the total clinical effective rate of the observation group (93.5%(29/31)) was significantly higher than that of the control group (72.7%(24/33)),and the difference between the two groups was statistically significant (χ2=4.868,P<0.05).Before treatment,difference between serum interleukin?6,interleukin?1β and interleukin?18 from two groups and each clinic indicators level has no statistic significance (all P>0.05) .After treatment,in the observation group,Serum IL?6 ( (16.68±6.83) ng/L and (32.24±6.99) ng/L,t=12.373),IL?1β((63.83 ±8.97)ng/L and (85.59±9.42) ng/L,t=9.758),IL?18((64.52±5.46) ng/L and (88.50±5.54) ng/Ll,t=18.899),24 h urinary protein quantification ( 0.3 (0.21,0.36) g/24 h and 1.0 (0.65,1.23) g/24 h,Z=-4.861),URBC (15.30 (3.80,36.80)×106/L and 168.9 (58.4, 324.0)×106/L,Z=-4.840),were lower than before treatment ( all P<0.05); After treatment, in the control group,Serum IL?6 ((23.62±5.95) pg/ml and (33.44±4.68) pg/ml,t=9.149),IL?1β ((68.67 ±6.31) pg/ml and (86.59±8.71) pg/ml,t=10.617),IL?18((71.25±9.69) pg/ml and (89.87±6.68) pg/ml,t=11.506),24 h urinary protein quantification (0.42 (0.33,0.56) g/24 h and 0.94 (0.74,1.25) g/24 h,Z=-5.013),URBC ( 57.00 ( 39.25,77.50)×106/L and 145.60 ( 58.20,360.85)×106/L,Z=-4.762),were lower than before treatment ( all P<0.05).The difference between the two groups was statistically significant (P<0.05).Conclusion The adjuvant effect of total glucoside of white peony root on hematuria and proteinuria purpura nephritis is significant, which can reduce the level of hematuria and proteinuria and improve the renal symptoms of children.Its mechanism of action may be to reduce renal inflammation and protect the kidney by down?regulating the expression of serum IL?6,IL?1 and IL?18.

16.
Article in Chinese | WPRIM | ID: wpr-858106

ABSTRACT

OBJECTIVE: To establish an HPLC method for simultaneous determination of 15 monoterpene glycosides in Radix Paeoniae Alba and seed cake of peony. METHODS: The separation was performed on an Agilent Zorbax SB C18-Aq column, using acetonitrile (A) and potassium dihydrogen phosphate solution (B) (pH 2.8) as the mobile phase by gradient elution. The elution program was as follows: 0 min(A, 9%)→8 min(A, 9%)→10 min(A, 15%)→25 min(A, 20%)→42 min(A, 35%)→50 min(A, 35%). The flow rate was 1.0 mL•min-1. The detection wavelength was maintained at 260 nm. The column temperature was set at 30 ℃. RESULTS: The linear range was 5.1-81.6 μg•mL-1 for pyrindylpaeoniflorin, 12.95-207.2 μg•mL-1 for mudanpioside F, 6.2-99.2 μg•mL-1 for oxyalbiflorin, 12.2-195.2 μg•mL-1 for oxypaeoniflorin, 8.0-128.0 μg•mL-1 for 10-hydroxypaeoniflorin, 5.5-88.0 μg•mL-1 for albiflorin, 6.0-96.0 μg•mL-1 for paeoniflorin, 6.0-96.0 μg•mL-1 for oxypaeonidanin, 5.6-89.6 μg•mL-1 for 4-O-methyl-oxypaeoniflorin, 4.7-75.2 μg•mL-1 for galloylpaeoniflorin, 5.4-86.4 μg•mL-1 for 4-O-methyl-paeoniflorin, 5.0-80.0 μg•mL-1 foralbiflorin R1, 5.7-91.2 μg•mL-1 for paeonidanin, 5.4-86.4 μg•mL-1 for benzoyloxypaeoniflorin and 6.7-107.2 μg•mL-1 for benzoylpaeoniflorin. The average recoveries of the 15 monoterpene glycosides, were between 96.8%-105.6% and the RSD values were 1.05%-3.41%. CONCLUSION: The method is simple, accurate and can be used for the quality control of peony.

17.
Article in Chinese | WPRIM | ID: wpr-851292

ABSTRACT

Objective This study focused on the secondary metabolites of endophytic fungus Alternaria alternate in Paeonia lactiflora. Methods Compounds were isolated from the EtOAc extract by chromatography technology and their structures were elucidated on the basis of comprehensive spectroscopic analysis. Results A total of 15 compounds were isolated and their structures were identified as methyl 4-acetamido-3-hydroxybenzoate (1), (E)-methyl5-hydroxy-3-methylpent-2-eno (2), isobenzofuranone A (3), 4-hydroxyacetophenone (4), 6-hydroxy-isosclerone (5), talarojlavone (6), 7-hydroxy-2-hydroxymethyl-5-methyl-4H-chromen-4-one (7), alternarienonic acid (8), 7-hydroxy-2,5-dimethyl-4H-1-benzopyran-4-one (9), 5-hydroxy-epialtenuene (10), alternariol (11), methyl 3-hydroxybenzoate (12), stemphyperylenol (13), altenusin (14), and altenuene (15). Conclusion Compounds 1, 3, 5-10, 12, and 13 are isolated from Alternaria alternatefor the first time.

18.
Article in Chinese | WPRIM | ID: wpr-850959

ABSTRACT

Paeoniflorin is the main effective component of Paeoniae lactiflora and Paeonia suffruticosa. A large number of studies have proven that paeoniflorin has many pharmacological effects, such as anti-depression, anti-inflammation, analgesia, anti-tumor, liver protection, nerve protection, sedation and hypnosis, immunomodulation and so on. It has little toxic and side effects and has been highly concerned by people. At present, paeoniflorin is rarely used in clinical practice in the form of monomers. This article mainly refers to the related research literatures on paeoniflorin pharmacological action in recent three years, combs and summarizes the new progress of its pharmacological action research. The relationship between the drug effect and the prescription of traditional Chinese medicine was discussed, in order to provide a reference for further development and clinical application of paeoniflorin.

19.
Article in Chinese | WPRIM | ID: wpr-845292

ABSTRACT

Objective: To establish the HPLC fingerprint of the chemical components of the decoction of Radix Paeoniae Alba(RPA)and determine the content of paeoniflorin, so as to provide a scientific method for the quality char- acterization and control of RPA and establish a correlation between the quality of standard granules in classical prescrip- tion and the quality of RPA. Methods: Thermo Syncronis C18(250 mm×4.6 mm, 5 μm)chromatographic column was used, the mobile phase was constituted by acetonitrile(A)and 0.05 % phosphate aqueous solution(B), the detection wavelength was 230 nm, the column temperature was 30℃, the flow rate was 1.0 ml/min, and the injection volume was 20 μl;the gradient eluting procedure was 0-15 min, 5% A-15% A;15-45 min, 15% A-25% A;45-65 min, 25% A-50% A. Results: The HPLC fingerprint of RPA was established, 11 common peaks were identified by HPLC-Q-TOFMS/ MS, and the similarity of 10 batches were evaluated. The content determination of paeoniflorin in 10 batches of RPA decoction-lyophilized powders was also completed. Conclusion: This study provides a scientific and reliable method for the quality characterization and control of RPA as well as a reference for the quality characterization and control of the standard decoction and granules.

20.
China Pharmacy ; (12): 2829-2834, 2019.
Article in Chinese | WPRIM | ID: wpr-817529

ABSTRACT

OBJECTIVE: To establish the method for simultaneous determination of 69 kinds of pesticide residues in Paeonia tactilora, Astragalus membranaceus, Ranunculus ternatus and Cornus officinalis. METHODS: GC-MS/MS method was adopted. The determination was performed on HP-5MS fused silica capillary column with splitless injecting samples. The injector temperature was set at 240 ℃, and sample size was 1 μL. The carrier gas was high-purity helium, the inlet mode was constant pressure, the pre-column pressure was 146 kPa, and the temperature was programmed. Triple four-pole tandem mass spectrometry was used for the detection, and electron impact ion source was used as ion source. The temperature of the ion source was 230 ℃, ionization energy was 70 eV, and the collision gas was nitrogen. The inlet temperature was 280 ℃ and four-pole temperature was 150 ℃, mass spectrometry monitoring mode was multi-reaction monitoring (MRM), and solvent delay time was 5 min. RESULTS: The linear range of 69 kinds of pesticide residue was 4.82-399.6 ng/mL (all r>0.990). LODs were all in the range of 0.001 7-0.013 3 mg/kg, and LOQs were all in the range of 0.000 5-0.004 mg/kg. RSDs of precision and stability tests were less than 10% (n=6). RSD of reproducibility test was lower than 5% (n=6, only pesticide amidine and permethrin were detected). The recoveries were in the range of 62.9%-123.5% (all RSD<10%, n=6). Among 12 batches of samples, dichlorvos and diphenylamine were detected in C. officinalis; chlordimeform and permethrin were detected in  A. membranaceus; diphenylamine and chlordimeform were detected in P. tactilora; diphenylamine and vinclozolin were detected in R. ternatus. CONCLUSIONS: The method is simple in operation and reproducible for simultaneous determination of 69 kinds of pesticide residue in   P. tactilora, A. membranaceus, R. ternatus and C. officinalis.

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