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Säo Paulo med. j ; 140(1): 12-16, Jan.-Feb. 2022. tab
Article in English | LILACS-Express | LILACS | ID: biblio-1357469


ABSTRACT BACKGROUND: Coronavirus disease 2019 (COVID-19) is an ongoing global health threat. However, currently, no standard therapy has been approved for the disease. OBJECTIVES: To evaluate the clinical effectiveness of convalescent plasma (CP) in patients with acute respiratory distress syndrome (ARDS) due to COVID-19. DESIGN AND SETTING: Retrospective study conducted at Kayseri City Education and Research Hospital, Kayseri, Turkey. METHODS: The case group consisted of adult patients (> 18 years) with ARDS due to COVID-19 who received CP in combination with antiviral and supportive treatment. These patients were compared with others who only received antiviral and supportive treatment. RESULTS: During the study period, a total of 30 patients with ARDS due to COVID-19 were included. Eleven patients (36%) received CP in combination with antiviral and supportive treatment, whereas nineteen patients (64%) in the control group only received antiviral and supportive treatment. On admission, the median age, demographic and clinical data and initial laboratory test results were similar between the groups (P > 0.05). On the 14th day of treatment, the laboratory values remained similar between the groups (P > 0.05). The mortality rates were not significantly different between the groups. CONCLUSION: CP treatment did not affect mortality or lead to clinical improvement for COVID-19 patients with ARDS.

Hematol., Transfus. Cell Ther. (Impr.) ; 44(1): 1-6, Jan.-Mar. 2022. tab, graf
Article in English | LILACS | ID: biblio-1364895


Abstract Introduction As the Coronavirus Disease 2019 (COVID-19) pandemic unfolds around the world; answers related to the antibody response against the virus are necessary to develop treatment and prophylactic strategies. We attempted to understand part of the immune response of convalescent plasma donation candidates. Method We carried out a cross-sectional, observational, non-intervention study, testing 102 convalescent plasma donation candidates for antibodies against the virus, relating these data to the time interval between symptom onset and sample collection, age, disease severity, and gender. Results In our sample, the individuals who developed a greater antibody response were the ones who had a longer time interval between symptom onset and sample collection, the ones who had been hospitalized and the subjects above 35 years old. Moreover, 17 individuals did not present any reactive antibodies. Conclusion These results are important in that they raise questions about the role of the humoral response against the virus, as some individuals do not develop antibodies to fight it. In addition, they help develop recruitment strategies for convalescent plasma donors, who should be asymptomatic for at least 21 days and are possibly more likely to have reactive antibodies after 35 days without symptoms.

Humans , Male , Female , Adolescent , Adult , Middle Aged , Young Adult , Coronavirus Infections , COVID-19 Serological Testing , COVID-19 , Plasma , Blood Donors , SARS-CoV-2
Journal of Preventive Medicine ; (12): 101-105, 2022.
Article in Chinese | WPRIM | ID: wpr-907092


Objective @#To establish a microwave-assisted digestion-inductively coupled plasma mass spectrometry (ICP-MS) with an octopole reaction system for simultaneous determination of six heavy metals in peanuts, including Cr, Ni, As, Cd, Pb, Hg. @*Methods @#Peanut samples were shelled and crushed evenly, and 0.350 0 g was accurately weighed and digested with 5 mL nitric acid and 1 mL hydrogen peroxide in a digestion tank. Following microwave-assisted digestion, pure water was used to quantify the samples, and internal standards and an octopole reaction system were used to remove the interference. Then, the contents of six heavy metals were determined in peanuts by ICP-MS. The accuracy and precision of ICP-MS were evaluated using national criteria ( GBW 10013 and GBW 10044 ) and spike-and-recovery testing. @*Results @#The six heavy metals showed good linearity at the selected linear range ( r≥0.999 8 ). The detection limits of ICP-MS ranged from 0.001 4 to 0.023 8 ng/mL, and the spike-and-recovery rates ranged from 94.7% to 98.8%, with the relative standard deviations ranging from 0.7% to 3.6%. In addition, the determination results of the standard reference materials were all within the normal reference range. The detection of six heavy metals was 100.0% in 60 peanut samples, and the contents of six heavy metals were all low.@*Conclusion @#The established ICP-MS assay is feasible for simultaneous determination of multiple heavy metals in peanuts.

Journal of Clinical Hepatology ; (12): 268-272, 2022.
Article in Chinese | WPRIM | ID: wpr-920866


Acute liver failure (ALF) is a rare and extremely severe clinical form of Wilson's disease (WD), characterized by progressive aggravation of jaundice and significant coagulation disorder with acute intravascular hemolysis. There is a high risk of severe complications such as hepatic encephalopathy and acute renal failure, and the disease progresses rapidly after onset and has a high mortality rate. At present, it is difficult to diagnose WD presenting as ALF in the early stage due to a lack of unified indicators for rapid diagnosis. Liver transplantation was considered the only effective treatment method for this disease in the past; however, recent studies have shown that medical treatment without liver transplantation can achieve autologous liver relief and recovery in some patients with WD-ALF.

Article in Chinese | WPRIM | ID: wpr-920655


@#An innovative approach to quantitatively analyze the histamine and its precursor histidine simultaneously in biological matrices was established for the first time based on double adsorption combined with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).The internal standard was 2-dihydroxybenzoic acid (DHB).The plasma and brain tissue homogenate was protein precipitated with 3-fold acetonitrile, and the supernatant was then sampled for injection analysis.The chromatographic separation of the target components was achieved on an amino chromatography column (ODS-SPXBridge? Amide).Gradient elution was carried out with the mobile phase consisting of solvent A (0.1% formic acid and 1mmol/L ammonium formate in water) and solvent B (acetonitrile).Mass spectrometry was employed for quantitative analysis with ESI ion source in multiple reaction monitoring (MRM) mode.In order to improve the specificity and accuracy, activated carbon and calcite were used for the double adsorption of biological matrices for the first time.The adsorbed matrix was then used for methodology validation.The results showed that histamine and histidine were linear in the quantitative range (correlation coefficient r ≥ 0.999).Accuracy, precision, extraction recovery, matrix effect and stability all met the requirements of biological sample analysis.All results suggested that the present method could not only be efficiently and reliably used for simultaneous quantitative analysis of histamine and histidine in biological samples, but also provide reference for the detection of other endogenous substances.

Article in Chinese | WPRIM | ID: wpr-920549


Objective@# To summarize the clinical manifestations of IgG4-related diseases in the head and neck, explore treatment methods.@*Methods @#The clinical data of 21 patients diagnosed with IgG4-related diseases were retrospectively analyzed. The clinical data and the results of glucocorticoid and immunosuppressive therapy were studied retrospectively.@* Results@#All patients had swollen sclerotic masses, and CT showed irregular high-density masses with uniform enhancement in the enlarged glands. Some patients had mucosal thickening and mass-like changes in theoral cavity, nose, sinuses, throat and other tissues, and most of the patients had cervical lymphadenopathy and elevated serum IgG4 levels (≥ 1.35 g/L). Histopathological examination of affected exosine glands and affected mucosa and lymph nodes in all patients showed infiltration of lymphocytes, plasma cells and IgG4+ plasma cells. In 21 patients, the mass in the affected glands and mucosa (including head, neck and other tissues) disappeared, and the clinical symptoms were relieved after the application of glucocorticoids. However, with a reduction in glucocorticoids, the mass recurred or even worsened.@*Conclusion @#For patients with a single mass in the submandibular gland, parotid gland and other salivary glands, as well as lymph node enlargement, CT is the first choice to identify the nature of gland neoplasms. Combined with pathological examination, related auxiliary examination and peripheral blood examination are also needed to obtain a definitive diagnosis. Glucocorticoid therapy is used to achieve a good prognosis, and long-term follow-up and timely adjustment of medication regimens are required.

International Eye Science ; (12): 485-489, 2022.
Article in Chinese | WPRIM | ID: wpr-920437


@#AIM: To observe the preliminary therapeutic effect of low-temperature plasma ablation combined with drugs in the treatment of infectious corneal ulcer. <p>METHODS: Retrospective case study. A total of 30 eyes were selected from 30 patients with infectious corneal ulcer who were admitted to the ophthalmology department of the First Affiliated Hospital of Chongqing Medical University from December 2018 to March 2020. All patients had positive etiological examination and ulcer or infiltration depth ≤1/2 of corneal thickness. Local low-temperature plasma ablation combined with eye drops was applied to the cases whose corneal ulcer did not improve significantly or corneal infiltration continued to worsen after 3-7d of conventional anti-infection treatment. Postoperative follow-up was 3-6mo to observe the clinical effect.<p>RESULTS: After low-temperature plasma ablation combined with eye drops treatment, the infection in 12 eyes of 12 patients with bacterial corneal ulcer was controlled in 11 eyes of 11 patients and ulcer healed gradually. Keratoplasty was performed in 1 eye due to aggravated infiltration. Among 18 eyes of 18 cases with fungal corneal ulcer receiving low-temperature plasma ablation and combined local antifungal treatment, 13 eyes of 13 cases gradually healed; 2 eyes of 2 patients showed lichen-like changes after the first ablation, but the lesion area was significantly smaller than before and gradually improved after re-ablation treatment; there was no effect in 3 eyes of 3 cases and keratoplasty was finally performed. All the patients were followed up for 3-6mo, 26 eyes of 26 patients were cured and the infection had no recurrence with remaining corneal pannus or leukoplakia. Confocal microscopy did not detect mycelium in cured patients with fungal infection.<p>CONCLUSION: In this preliminary observation, low-temperature plasma ablation combined with drug therapy can effectively control infection, promote healing and improve visual acuity for infective corneal ulcer with infiltration less than 1/2 corneal thickness, and no obvious complications were observed.

Einstein (Säo Paulo) ; 20: eRC6550, 2022. graf
Article in English | LILACS-Express | LILACS | ID: biblio-1360401


ABSTRACT The coronavirus disease 19 (COVID-19) is responsible for the current worldwide pandemic. Treatment and prophylaxis are still under investigation. Convalescent plasma therapy could be an alternative. We report a case of a 41-year-old patient, at 28 weeks of gestation, was hospitalized with COVID-19. On the 10th day after onset of symptoms, the clinical picture worsened, and she required high-flow oxygen therapy (30L/minute), with 92% oxygen saturation, and chest X-ray showing mild bilateral opacities at lung bases. Blood tests showed D-dimer 1,004ng/mL, C-reactive protein 81mg/L, pro-calcitonin 0.05ng/mL and interleukine-6 42.9pg/mL. The therapy chosen was Tazocin® 12g/day, vancomycin 2g/day, and methylprednisolone 40mg/day. In addition, convalescent plasma therapy was administered (275mL) uneventfully, including SARS-CoV-2 antibodies and neutralizing antibodies >1:160. The patient had a fast recovery. The early administration of convalescent plasma, with high titers of neutralizing antibodies, may be an alternative option for severe COVID-19 during pregnancy, until further studies demonstrate an efficient and safe treatment or prophylaxis.

Ann. Health Res. (Onabanjo Univ. Teach. Hosp.) ; 8(1): 40-48, 2022. figures, tables
Article in English | AIM, AIM | ID: biblio-1362986


Background: The cessation of ovarian functions at menopause and the accompanying decline in the production of ovarian steroid hormones creates a unique set of health concerns for women. Reductions in sex steroid levels, particularly oestrogen, have been associated with various diseases and conditions, including bleeding disorders, coronary heart disease (CHD), osteoporosis, cognitive dysfunction, urinary incontinence, hot flushes, and mood changes, among others. Objective: To determine changes in haemorheological and clotting profile in post-menopausal women. Methods: Two hundred participants comprising one hundred and fifty post-menopausal women and fifty healthy pre-menopausal control subjects were studied. The investigations carried out include whole blood viscosity, plasma viscosity, fibrinogen concentration, Prothrombin time (PT), Activated partial thromboplastin time with kaolin (APTTK) levels and complete blood count using standard methods. Results: The mean age (p=0.01), platelet count (p= 0.013), neutrophil (p= 0.03), neutrophil to lymphocyte ratio (p= 0.045) and platelet to lymphocyte ratio (p=0.044) in postmenopausal women were significantly higher while lymphocyte count (p= 0.004) was significantly lower in postmenopausal compared to premenopausal women. Similarly, plasma oestradiol (p= 0.001), plasma viscosity (p= 0.03), relative blood viscosity (p= 0.03), whole blood viscosity (p= 0.03) and PTTK(p= 0.04) were significantly lower among postmenopausal women compared to premenopausal control subjects. Conclusion: Relative plasma viscosity correlated positively with age. There were significantly lower levels of haemorheological and clotting profile in post-menopausal women. These changes may be due to age or a decline in circulating oestrogen levels.

Humans , Female , Blood Coagulation , Blood Viscosity , Menopause , Postmenopause
Rev. cuba. anestesiol. reanim ; 20(3): e751, 2021. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1351978


Introducción: La epicondilitis constituye uno de los motivos de consulta más frecuentes tanto en la asistencia primaria como especializada y sin duda alguna, es uno de los problemas que tiene mayor repercusión en la persona que la padece. El tratamiento de las epicondilitis constituye un reto para la medicina debido a enormes implicaciones sanitarias, sociolaborales y el dolor e impotencia funcional que provoca. Objetivo: Evaluar la efectividad del lisado plaquetario autólogo como alternativa de tratamiento en pacientes enfermos con epicondilitis. Método: Se realizó un estudio cuasi experimental analítico longitudinal prospectivo en el que se evaluó el uso de lisado plaquetario autólogo como alternativa de tratamiento en pacientes con epicondilitis. El universo estuvo constituido por los pacientes que acudieron a consulta de Ortopedia y traumatología con el diagnóstico de epicondilitis, durante el periodo comprendido entre octubre de 2014 y julio de 2018. La muestra quedo constituida por 80 pacientes que cumplieron con los criterios de inclusión y exclusión. Resultados: El grupo de edad entre 36-56 años y del sexo femenino son los de mayor representación en padecer esta enfermedad. Las infiltraciones de lisado plaquetario autólogo aportan mejores resultados al convencional y se observa la mayor representación de pacientes que tuvieron una remisión total. Las complicaciones fueron mucho más evidentes en el tratamiento convencional. También es relevante el costo-beneficio del tratamiento con lisado plaquetario autólogo. Conclusiones: El tratamiento con lisado plaquetario autólogo puede ser una alternativa para mejorar la calidad de vida de los pacientes con epicondilitis(AU)

Introduction: Epicondylitis is one of the most frequent reasons for attending consultation in both primary and specialized care; while it is undoubtedly one of the problems with the greatest impact on the person who suffers from it. The managment epicondylitis is a challenge for medicine, due to the enormous health-related and social implications, as well as the pain and functional impotence that it causes. Objective: To assess the effectiveness of autologous platelet lysate as a treatment alternative in patients with epicondylitis. Method: A prospective, longitudinal, analytical and quasiexperimental study was carried out, in which the use of autologous platelet lysate as an alternative treatment in patients with epicondylitis was assessed. The universe consisted of patients who attended the orthopedics and traumatology consultation, during the period between October 2014 and July 2018, with a diagnosis of epicondylitis. The sample was made up of eighty patients who met the inclusion criteria; exclusion criteria were also considered. Results: The age group between 36 and 56 years, together with the female sex, are the most represented with respect to suffering from this disease. Infiltrations of autologous platelet lysate provide better outcomes than the conventional one, while greater representation of remitted patients is observed. Complications were much more evident in conventional treatment. The cost-benefit relationship of treatment with autologous platelet lysate is also relevant. Conclusions: Treatment with autologous platelet lysate can be an alternative to improve the quality of life of patients with epicondylitis(AU)

Humans , Orthopedics , Primary Health Care , Quality of Life , Blood Platelets/physiology , Traumatology , Referral and Consultation
Rev. medica electron ; 43(5): 1409-1417, 2021.
Article in Spanish | LILACS | ID: biblio-1352120


RESUMEN El envejecimiento es un proceso complejo que trae consigo cambios celulares, histológicos y cutáneos. Estos últimos son una de sus manifestaciones más evidentes. El plasma rico en plaquetas es una fuente fiable de obtención de células para regenerar tejidos; por su fácil disponibilidad es un material inocuo. La bioestimulación con el mismo, por su parte, es un conjunto de procedimientos para activar las funciones anabólicas de los fibroblastos, producción de colágeno, elastina y ácido hialurónico. La tendencia al empleo de este en tratamientos antiedad es cada vez mayor. El objetivo de este trabajo fue realizar una actualización del tema, para exponer aspectos importantes sobre formas de aplicación, indicaciones, complicaciones y contraindicaciones. Existen varios métodos para la bioestimulación facial, tales como la realización de pápulas, napagge y retroinyección. Se han empleado en alopecia androgénica, areata, envejecimiento cutáneo, etc. Las complicaciones más observadas son dolor, eritema, ardor y sangrado local. Entre las contraindicaciones más comunes se observan el herpes simple recidivante, coagulopatías, tratamiento con anticoagulantes, colagenopatías y neoplasias (AU).

ABSTRACT Aging is a complex process that brings with it cellular, histological and cutaneous changes, the latter being one of its most obvious manifestations. Platelet-rich plasma is a reliable source of cells to regenerate tissues; due to its easy availability, it is a harmless material. Bio-stimulation with it is a set of procedures to activate the fibroblasts anabolic functions and the production of collagen, elastin and hyaluronic acid. The tendency to use it in anti-aging treatments increases faster and faster. The objective of this work was updating the topic to expose important aspects about application methods, indications, complications and contraindications. There are several methods of applying facial bio-stimulation such as performing papules, napagge, and retroinjection. It has been used in androgenic alopecia, alopecia areata, cutaneous ageing, etc. The most commonly found complications are pain, erythema, burning and local bleeding. The most common contraindications include recidivist herpes simplex, coagulopaties, anticoagulant treatment, collagen-related diseases and neoplasms (AU).

Humans , Male , Female , Platelet-Derived Growth Factor/therapeutic use , Dermatology/methods , Platelet-Derived Growth Factor/biosynthesis , Skin Aging/drug effects , Blood-Derivative Drugs
An. bras. dermatol ; 96(5): 544-550, Sept.-Oct. 2021. tab, graf
Article in English | LILACS | ID: biblio-1345153


Abstract Background: The treatment of cutaneous leishmaniasis is a challenge. A better understanding of the in situ mechanisms involved in the evolution and cure of the disease is essential for the development of new therapies. Objective: Correlate histopathological and immunological characteristics of cutaneous leishmaniasis lesions with clinical outcome after different treatment regimens. Methods: The authors analyzed cellular infiltration and immunohistochemistry staining for CD4, CD8 and IL-17 in biopsy samples from 33 patients with cutaneous leishmaniasis before treatment. All patients were recruited in a randomized clinical trial at Corte de Pedra (Bahia-Brazil) and assigned to receive Glucantime®, Glucantime® + Oral Tamoxifen or Glucantime® + Topical Tamoxifen. Patients were followed for 2 to 6 months to define disease outcome. Results: A similar expression of CD4, CD8 and IL-17 was observed in lesion samples regardless of clinical outcome. In general, a higher amount of CD8 cells were observed compared with CD4 cells. An important observation was that all patients whose cellular infiltrate did not contain plasma cells were cured after treatment. Study limitations: Isolated quantification of TCD8 and IL-17 using immunohistochemistry is insufficient to analyze the role of these molecules in the immunopathogenesis of cutaneous leishmaniasis. In addition, the expansion of the immunohistochemistry panel would allow a more complete analysis of the immune response in situ. Conclusions: The absence of plasma cells in cutaneous leishmaniasis lesions was related to a favorable therapeutic outcome.

Humans , Leishmaniasis, Cutaneous/drug therapy , CD4-Positive T-Lymphocytes , Treatment Outcome , CD8-Positive T-Lymphocytes , Meglumine Antimoniate
Colomb. med ; 52(3): e2054600, July-Sept. 2021. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1360376


Abstract Background: Multiple infections, nutrient deficiencies and inflammation (MINDI) occur in indigenous communities, but their associations with perinatal outcomes have not been described. Objective: To assess maternal and cord blood micronutrient and inflammation status in peripartum mothers from the Ngäbe-Buglé comarca in Panama, and their associations with placental and infant outcomes. Methods: In 34 mother-newborn dyads, placental weight and diameter were measured, and maternal and cord blood were processed for complete cell counts, serum C-reactive protein, ferritin, serum transferrin receptor (sTfR), vitamins A and D. Blood volumes were calculated using Nadler's formula. Results: Mothers had low plasma volume (<2.8 L, 96%), vitamin A (52.9%), vitamin D (29.4%), iron (58.8%) and hemoglobin (23.5%), but high hematocrit (>40%, 17.6%) and inflammation (C-reactive protein >8.1 mg/L, 85.3%). Birthweights were normal, but low placental weight (35.3%), low head circumference Z-scores (17.6%), and low cord hemoglobin (5.9%), iron (79.4%), vitamin A (14.7%) and vitamin D (82.3%) were identified. Maternal and cord vitamin D were highly correlated. Higher maternal plasma volume was associated with heavier placentae (β= 0.57), and higher cord D (β= 0.43) and eosinophils (β= 0.43) with larger placentae. Hemoconcentration (higher cord hematocrit) was associated with lower newborn weight (β= -0.48) and head circumference (β= -0.56). Inflammation [higher maternal neutrophils (β= -0.50), and cord platelets (β= -0.32)] was associated with lower newborn length and head circumference. Conclusion: Maternal-newborn hemoconcentration, subclinical inflammation and multiple nutrient deficiencies, particularly neonatal vitamin D deficiency, were identified as potential targets for interventions to improve pregnancy outcomes in vulnerable communities.

Resumen Antecedentes: Las Múltiples Infecciones, Nutrición Deficiente e Inflamación (MINDI), son frecuentes en comunidades indígenas, sin embargo, sus asociaciones con resultados de salud perinatales no han sido descritos. Objetivo: Evaluar la inflamación y los micronutrientes en sangre materna y de cordón de madres en trabajo de parto en la comarca Ngäbe-Buglé en Panamá, así como sus asociaciones con medidas placentarias y del recién nacido. Métodos: En 34 pares madre-recién nacido, se midieron peso y diámetro placentario, y se analizaron muestras de sangre materna y de cordón umbilical para hemograma completo, proteína-C reactiva (PCR), ferritina, receptor sérico de transferrina (RsTf), vitaminas A y D. Se usó la fórmula de Nadler para calcular volúmenes sanguíneos. Resultados: Las madres presentaron volumen plasmático (<2.8 L, 96%), vitamina A (52.9%), vitamina D (29.4%), hierro (58.8%) y hemoglobina (23.5%) bajos, pero el 17.6% presentaron hematocrito >40% y 85.3% presentaron inflamación (PCR >8.1 mg/L). Los pesos al nacer fueron normales, pero se identificó bajo peso placentario (35.3%), bajo puntaje-z de circunferencia cefálica neonatal, y en sangre de cordón, bajos hemoglobina (5.9%), hierro (79.4%), vitamina A (14.7%) y vitamina D (82.3%). Se encontró una fuerte correlación positiva entre la vitamina D materna y de sangre de cordón. Un mayor volumen plasmático materno se asoció con placentas de mayor peso (β= 0.57), en tanto que concentraciones más altas de vitamina D (β= 0.43) y mayor número de eosinófilos (β= 0.43) se asociaron con mayor diámetro placentario. Una mayor hemoconcentración (hematocrito en cordón más alto) se asoció con menores peso al nacer (β= -0.48) y circunferencia cefálica (β= -0.56). La inflamación [mayor número de neutrófilos maternos (β= -0.50) y plaquetas en sangre de cordón (β= -0.32)] se asoció con menor talla y circunferencia cefálica neonatales. Conclusión: La hemoconcentración materna y del recién nacido, la inflamación subclínica y las múltiples deficiencias en micronutrientes, particularmente la deficiencia de vitamina D neonatal, se identificaron como potenciales áreas de intervención para mejorar los resultados de salud del embarazo en comunidades vulnerables.

Rev. Ciênc. Méd. Biol. (Impr.) ; 20(2): 349-356, set 29, 2021.
Article in Portuguese | LILACS | ID: biblio-1354668


Introdução: o processo de senescência do indivíduo ocorre de forma lenta e contínua e pode gerar inúmeras alterações, dentre elas o envelhecimento cutâneo, derivado do declínio das atividades celulares. Inúmeros recursos têm sido criados ao longo dos anos com a finalidade de frear e ou reverter os aspectos inestéticos da pele, ocasionados por esse processo. Um novo recurso terapêutico vem sendo usado com o objetivo de promover o rejuvenescimento através da diminuição de rugas e linhas de expressão, trata-se do Jato de plasma. Objetivo: avaliar o padrão de variação térmica do tecido tratado com jato de plasma, através da termografia infravermelha. Metodologia: trata-se de uma série de casos clínicos que envolveu 5 pacientes mulheres, com idade acima de 40 anos que apresentavam rugas faciais. As pacientes foram avaliadas e anestesiadas previamente. Em seguida, foi realizada a terapia com o jato de plasma, na região supraorbital. As pacientes foram avaliadas termograficamente antes e depois da anestesia e pósterapia. Resultado: constatou-se através da análise termográfica, uma significativa variação no coeficiente de temperatura da pele onde foi aplicado o anestésico e em seguida o jato de plasma (ΔT > 0,4 °C), em todas as pacientes. Conclusão: o presente estudo comprovou através da utilização da termografia, que o jato de plasma foi capaz de gerar um aumento da temperatura local. Os autores sugerem que tal variação térmica pode ser resultante de um processo de vasodilatação na região tratada.

Introduction: individual's senescence process takes place slowly and continuously beyond generates several changes including skin aging, since the decline in cellular activities. Countless resources have been created over the years, with the goal of stopping and / or reversing the unsightly aspects of the skin caused by this process. Thus, plasma jet, a new therapeutic resource has been used in order to promote rejuvenation through the reduction of wrinkles and expression lines. Objective: To evaluate the pattern of thermal variation of the tissue treated with a plasma jet, using infrared thermography. Methodology: this was a series of clinical cases involving 5 female patients over the age of 40 who had facial wrinkles. Patients were previously evaluated and anesthetized. Then, plasma jet therapy was performed in the supra-orbital region. Patients were evaluated for thermal imaging before and after anesthesia and post-therapy. Result: it was found through thermographic analysis, a significant variation in the temperature coefficient of the skin of all patients where the anesthetic and plasma jet were applied (ΔT > 0.4 °C). Conclusion: the present study proved through the use of thermography that the plasma jet was able to generate an increase in local temperature. The authors suggest that such thermal variation may be the result of a process of vasodilation in the treated region.

Humans , Female , Adult , Rejuvenation , Thermography , Case Reports , Epidemiology, Descriptive
Rev. cuba. oftalmol ; 34(3): e925, 2021.
Article in Spanish | LILACS, CUMED | ID: biblio-1352031


La enfermedad de la superficie ocular incluye a un grupo de patologías con diversas etiologías, síntomas y hallazgos clínicos que comparten la producción de reacción inflamatoria y daño de esta superficie. El uso de derivados hemáticos para el tratamiento de patologías de la superficie ocular se ha incrementado en el área de la oftalmología, ya que su composición es análoga a la de la lágrima natural. Con el objetivo de mostrar la terapia celular como una nueva disciplina científica a aplicar en nuestro medio, se realizó una búsqueda automatizada sobre el tema, teniendo en cuenta las publicaciones de los últimos 5 años. Se utilizó la plataforma Infomed, cuya información fue resumida para la elaboración del informe final, donde se expone que los colirios de hemoderivados proveen estrategias de tratamiento eficaces y seguras para pacientes con afecciones oftálmicas. El colirio de plasma rico en plaquetas ofrece una opción exitosa de tratamiento en numerosas afecciones de la superficie ocular. Sin embargo, estudios adicionales son necesarios para establecer la seguridad y la eficacia de este tipo de terapias(AU)

Ocular surface diseases are a group of conditions of different etiologies, symptoms and clinical findings with the common features of developing an inflammatory reaction and damaging the ocular surface. Use of blood-derived products for the treatment of ocular surface disorders has increased in ophthalmic care, since their composition is similar to that of natural tears. With the purpose of presenting cell therapy as a new scientific discipline that could be used in our environment, an automated search was conducted about the topic which included publications from the last five years. The search was performed on the Infomed platform, and the information obtained was summarized into a final report stating that blood-derived eye drops provide effective and safe treatment strategies for patients with ophthalmic conditions. Platelet-rich plasma eye drops are a potentially successful treatment option for many ocular surface disorders. However, further studies are required to establish the safety and effectiveness of this type of therapy(AU)

Humans , Ophthalmic Solutions/adverse effects , Blood-Derivative Drugs , Platelet-Rich Plasma/drug effects , Review Literature as Topic , Databases, Bibliographic , Research Report
Rev. cuba. med ; 60(3): e1383, 2021.
Article in Spanish | LILACS, CUMED | ID: biblio-1347520


Introducción: El nuevo coronavirus tiene material genético. Esto permite a un receptor transformarse en el sitio de acción polibásica y es capaz de infectar a través de múltiples receptores de entrada y resaltar a las proteínas de tipo espiga (S). La proteína 'espiga' es una proteína de fusión viral de la Covid-19, es por ello que en la búsqueda terapéutica se establece las siguientes preguntas, ¿la hibridación de anticuerpos logrará ganar la guerra contra la pandemia de la Covid-19?; o ¿el plasma rico en anticuerpos puede mantener a las personas fuera de las unidades de cuidados intensivos? Estas preguntas radican en que los estudios actuales no establecen la verdadera utilidad de la terapia inmunológica. Objetivo: Presentar la utilidad de anticuerpos híbridos ante la actual pandemia de la Covid-19 y otros coronavirus. Métodos: Se desarrolló una revisión bibliográfica a partir de la evidencia existente acerca del panorama de la proteómica en el estudio del sistema inmune para combatir infecciones. Se utilizó un margen de tiempo entre el año 1999 al 2020. Se seleccionaron un total de 37 documentos que cumplen con los protocolos de inclusión en idioma inglés o español; en la búsqueda se utilizaron términos MeSH. Se escogieron estudios de orden observacional o analíticos; de carácter experimental, reporte de casos que dataran aspectos bioquímicos, biológicos, patológicos y clínicos del sistema inmune como blanco terapéutico ante la pandemia actual. El análisis documental fue realizado por el Grupo de Investigación en Salud de la Universidad del Cauca-Popayán, con apoyo y dirección de la Universidad de Houston, Texas (EEUU), con el apoyo de profesorado del Programa de Investigación Humana de la NASA. Resultados: La respuesta positiva para controlar esta pandemia está basada en los cuidados preventivos y en las posibles terapias innovadoras ante los nuevos coronavirus que logren transmitirse de animales a humanos. Se resalta el posible uso de anticuerpos de dominio único híbridos para frenar infecciones víricas nuevas. Conclusiones: Se resalta el posible uso de anticuerpos de dominio único híbridos para frenar infecciones víricas nuevas(AU)

Introduction: The new coronavirus has genetic material. This allows a receptor to transform into the polybasic site of action and it is capable of infecting through multiple entry receptors and highlighting spike-like (S) proteins. The spike protein is a viral fusion protein of Covid-19, which is why in the therapeutic search the following questions are established, will antibody hybridization succeed in winning the war against the Covid-19 pandemic? Or can antibody-rich plasma keep people out of intensive care units? These questions are that current studies do not establish the true utility of immune therapy. Objective: To settle the usefulness of hybrid antibodies to the current Covid-19 pandemic and other coronaviruses. Methods: A bibliographic review was developed from the existing evidence about the panorama of proteomics in the study of the immune system to fight infections. A time frame was used between 1999 and 2020. A total of 37 documents, in English or Spanish, that comply with the inclusion protocols were selected; MeSH terms were used in the search. Observational or analytical studies were chosen; experimental, case report dating biochemical, biological, pathological and clinical aspects of the immune system as a therapeutic target in the current pandemic. The documentary analysis was carried out by the Health Research Group of the Universidad del Cauca-Popayán, with the support and direction of the University of Houston, Texas (USA), with the support of faculty from NASA Human Research Program. Results: The positive response to control this pandemic is based on preventive care and possible innovative therapies for the new coronaviruses that manage to be transmitted from animals to humans. Conclusions: The possible use of hybrid single domain antibodies to stop new viral infections is highlighted(AU)

Humans , Immunoglobulins/therapeutic use , COVID-19/transmission , Antibodies, Viral/therapeutic use
Rev. méd. Urug ; 37(3): e37302, set. 2021. tab, graf
Article in Spanish | LILACS, BNUY | ID: biblio-1341550


Resumen: Las úlceras de pie diabético (UPD) generan un alto costo para el paciente y el sistema de salud. Una deficiente vascularización, la inhibición de la generación de factores de crecimiento y la migración celular, entre otros factores, influyen en su cronicidad. Los factores de crecimiento derivados de las plaquetas (PDGF) estimulan la quimiotaxis, la neovascularización y la regeneración tisular. Objetivo: evaluar la efectividad de una formulación de plasma rico en plaquetas autólogo (PRP) como una modalidad complementaria para el tratamiento de UPD, valorando su seguridad y efectos adversos. Materiales: estudio observacional. Se aplicó PRP a seis pacientes con UPD crónicas, Wagner II, III, con una media en el retraso de la cicatrización de 94 semanas. Se administró mediante punción y como gel sobre la lesión una vez por semana hasta el cierre de la úlcera o durante12 semanas. Las UPD se analizaron con respecto al área y su progresión mediante Mobile Wound Analyzer (MOWA), software de análisis de imágenes de úlceras. El porcentaje de reducción del área se calculó entre la medición inicial y la semana 12. Como criterio de mejoría se eligió una reducción >70% de la lesión. Resultados: predominó el sexo masculino con una media de edad de 53 años ± 7,3. Luego de 12 semanas de tratamiento, se reportó una reducción media del tamaño de la úlcera de 78%, con dos cicatrizaciones completas y dos en 99%. Un paciente recibió una amputación luego de presentar infección no controlada. Conclusión: la aplicación del PRP mejoró la regeneración de tejidos, acortando la duración de la úlcera, promoviendo su curación sin efectos adversos, al tiempo que eliminó la necesidad de procedimientos de manejo en una úlcera crónica.

Summary: Diabetic foot ulcers imply high expenditure for both patients and the health system. Vascular impairment, growth factor inhibition and cell migration, among other factors, affect the chronicity of the condition. Platelet-derived growth factors (PDGFs) stimulate chemotaxis, neovascularization and tissue regeneration. Objective: to evaluate effectiveness of a formulation of autologous platelet-rich plasma to complement the treatment of diabetic foot ulcer, assessing safety and adverse effects. Material: observational study. Platelet rich plasma was applied to six patients with chronic diabetic foot ulcer Wagner II, III, with an average delayed healing of 94 weeks. PRP gel was administered by puncture technique on the lesion, once a week until the ulcer healed or for 12 weeks. Diabetic foot ulcers were analysed in regards to surface area and progression through the Mobile Wound Analyzer (MOWA) software to analyse ulcer images. The area reduction percentage was calculated comparing the initial measurement and that of week 12. The improvement criteria was defined in a reduction that is >70% of the lesion. Results: men prevailed with an average age of 53 years ± 7.3. After 12 weeks of treatment an average reduction of 78% of the size of the ulcer was reported, two complete healings and two 99% healed. One patient required amputation because of uncontrolled infection. Conclusion: the application of PRP improved tissue regeneration and shortened the duration of the ulcer, promoting healing with no adverse effects, and it likewise eliminated the need for procedures to handle chronic ulcers.

Resumo: As úlceras do pé diabético (UPD) geram alto custo para o paciente e para o sistema de saúde. A vascularização deficiente, a inibição da geração de fatores de crescimento e migração celular, entre outros fatores, influenciam sua cronicidade. Os fatores de crescimento derivados de plaquetas (PDGF) estimulam a quimiotaxia, a neovascularização e a regeneração de tecidos. Objetivo: avaliar a eficácia de uma formulação autóloga de plasma rico em plaquetas (PRP) como modalidade complementar para o tratamento da UPD, avaliando sua segurança e efeitos adversos. Materiais: estudo observacional. O PRP foi aplicado a 6 pacientes com UPD crônica, Wagner II, III, com um atraso médio na cicatrização de 94 semanas. Foi administrado por punção e como gel sobre a lesão uma vez por semana até o fechamento da úlcera ou por 12 semanas. As UPD foram analisadas em relação à área e sua progressão, utilizando o software de análise de imagem de úlcera Mobile Wound Analyzer (MOWA). A redução percentual da área foi calculada entre a medição inicial e a semana 12. Como critério de melhora, optou-se pela redução > 70% da lesão. Resultados: predominou o sexo masculino com média de idade de 53 ± 7,3 anos. Após 12 semanas de tratamento, foi relatada uma redução média no tamanho da úlcera de 78%, sendo 2 com cicatrização completa e 2 em 99%. Um paciente foi amputado após desenvolver infecção descontrolada. Conclusão: a aplicação do PRP melhorou a regeneração tecidual, encurtando o tempo de duração da úlcera, promovendo sua cicatrização sem efeitos adversos, ao mesmo tempo que eliminava a necessidade de procedimentos de manejo na úlcera crônica.

Diabetic Foot/therapy , Platelet-Rich Plasma , Foot Ulcer/therapy
Hematol., Transfus. Cell Ther. (Impr.) ; 43(2): 179-184, Apr.-June 2021. tab, graf
Article in English | LILACS | ID: biblio-1286689


ABSTRACT Background Transfusion of platelets (PLTs) with high ABO antibody titres can pose a risk of hemolysis if the unit crosses the ABO type. The PLTs stored in the platelet additive solution (PAS) remove asubstantial fraction of plasma and replace it with an isotonicbuffered solution.We aimed to assess the difference in anti-A/B antibody levels in Groups O, A and B apheresis platelets (APs) suspended in plasma and PAS. Methodology Apheresis donors are categorized into two groups, Plasma (Group I) and PAS (Group II), each blood group (A, B and O) had 20 samples. The anti-A/B(IgM)antibody levels were recorded from the AP donor (Group II) and from the AP units for both groups. The reduction in the anti-A/B(IgM) antibody levels in the APs suspended in the PAS for each blood group was determined. Results The median anti-A titres in blood Groups B (p = 0.009) and O (p = 0.005) was significantly lower in Group II. However, the difference in anti-B levels was not significant in the blood groups A (p = 0.057) and O (p = 0.205). The median level of reduction in IgM antibody titres across donor samples and the PAS-stored platelets was two-fold. The regression showed a level of reduction in antibody titres which can be explained by baseline donor antibody titres in blood groups A and B compared to blood group O. Conclusion The medianABO antibody titres were lower in APs suspended in PAS than in plasma. Addition of the PAS significantly lowered the IgM antibody titres by twofold, compared to plasma.

Humans , Plasma , Blood Component Removal , ABO Blood-Group System , Platelet Transfusion
Hematol., Transfus. Cell Ther. (Impr.) ; 43(2): 201-211, Apr.-June 2021. tab, ilus
Article in English | LILACS | ID: biblio-1286682


ABSTRACT The COVID-19 pandemic has pushed the world towards social, economic, and medical challenges. Scientific research in medicine is the only means to overcome novel and complex diseases like COVID-19. To sum up the therapeutic wild-goose chase, many available antivirals and repurposed drugs have failed to show successful clinical evidence in patient recovery, several vaccine candidates are still waiting in the trial pipelines and a few have become available to the common public for administration in record time.However, with upcoming evidence of coronavirus mutations, available vaccines may thrive on the spirit of doubt about efficacy and effectiveness towards these new strains of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV2). In all these collective uncertainties, plasma therapy has shown a ray of hope for critically ill patients. To date, with very few published case studies of convalescent plasma in COVID-19, there are two school of thought process in the scientific community regarding plasma therapy efficiency and this leads to confusion due to the lack of optimal randomized and controlled studies.Without undertaking any robust scientific studies, evidence or caution, accepting any therapy unanimously may cause more harm than good, but with a clearer understanding of SARS-CoV2 immunopathology and drug response, plasma therapy might be the silver lining against COVID-19 for the global community.

Plasma , COVID-19/therapy
Cuad. Hosp. Clín ; 62(1): 63-71, jun. 2021. ilus
Article in Spanish | LILACS | ID: biblio-1284353


La enfermedad de Addison es una patología endocrinológica ocasionada por la disminución en la secreción de hormonas esteroideas por parte de la corteza adrenal; debida a múltiples etiologías (más comúnmente la tuberculosis en países en vías de desarrollo); con una evolución lenta, insidiosa y progresiva. Pudiendo llegar a una insuficiencia adrenal aguda, misma que puede llegar a ser de extrema gravedad; y en caso de no ser diagnosticada y tratada adecuadamente puede llevar a la muerte. El tratamiento primordial lo constituye la terapia hormonal sustitutiva con fármacos corticoesteroideos.

Addison's disease is an endocrinological pathology caused by the decrease in the secretion of steroid hormones by the adrenal cortex, due to multiple etiologies (most commonly tuberculosis in developing countries); with a slow, insidious and progressive evolution. Being able to reach an acute adrenal insufficiency, which can become extremely serious, and if it is not diagnosed and treated properly, it can lead to death. The primary treatment is hormone replacement therapy with corticosteroid drugs

Humans , Female , Adult , Addison Disease , Adrenal Cortex Hormones , Adrenal Insufficiency , Tuberculosis , Hormone Replacement Therapy , Hormones , Hypothyroidism