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1.
Rev. cuba. anestesiol. reanim ; 20(3): e751, 2021. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1351978

ABSTRACT

Introducción: La epicondilitis constituye uno de los motivos de consulta más frecuentes tanto en la asistencia primaria como especializada y sin duda alguna, es uno de los problemas que tiene mayor repercusión en la persona que la padece. El tratamiento de las epicondilitis constituye un reto para la medicina debido a enormes implicaciones sanitarias, sociolaborales y el dolor e impotencia funcional que provoca. Objetivo: Evaluar la efectividad del lisado plaquetario autólogo como alternativa de tratamiento en pacientes enfermos con epicondilitis. Método: Se realizó un estudio cuasi experimental analítico longitudinal prospectivo en el que se evaluó el uso de lisado plaquetario autólogo como alternativa de tratamiento en pacientes con epicondilitis. El universo estuvo constituido por los pacientes que acudieron a consulta de Ortopedia y traumatología con el diagnóstico de epicondilitis, durante el periodo comprendido entre octubre de 2014 y julio de 2018. La muestra quedo constituida por 80 pacientes que cumplieron con los criterios de inclusión y exclusión. Resultados: El grupo de edad entre 36-56 años y del sexo femenino son los de mayor representación en padecer esta enfermedad. Las infiltraciones de lisado plaquetario autólogo aportan mejores resultados al convencional y se observa la mayor representación de pacientes que tuvieron una remisión total. Las complicaciones fueron mucho más evidentes en el tratamiento convencional. También es relevante el costo-beneficio del tratamiento con lisado plaquetario autólogo. Conclusiones: El tratamiento con lisado plaquetario autólogo puede ser una alternativa para mejorar la calidad de vida de los pacientes con epicondilitis(AU)


Introduction: Epicondylitis is one of the most frequent reasons for attending consultation in both primary and specialized care; while it is undoubtedly one of the problems with the greatest impact on the person who suffers from it. The managment epicondylitis is a challenge for medicine, due to the enormous health-related and social implications, as well as the pain and functional impotence that it causes. Objective: To assess the effectiveness of autologous platelet lysate as a treatment alternative in patients with epicondylitis. Method: A prospective, longitudinal, analytical and quasiexperimental study was carried out, in which the use of autologous platelet lysate as an alternative treatment in patients with epicondylitis was assessed. The universe consisted of patients who attended the orthopedics and traumatology consultation, during the period between October 2014 and July 2018, with a diagnosis of epicondylitis. The sample was made up of eighty patients who met the inclusion criteria; exclusion criteria were also considered. Results: The age group between 36 and 56 years, together with the female sex, are the most represented with respect to suffering from this disease. Infiltrations of autologous platelet lysate provide better outcomes than the conventional one, while greater representation of remitted patients is observed. Complications were much more evident in conventional treatment. The cost-benefit relationship of treatment with autologous platelet lysate is also relevant. Conclusions: Treatment with autologous platelet lysate can be an alternative to improve the quality of life of patients with epicondylitis(AU)


Subject(s)
Humans , Orthopedics , Primary Health Care , Quality of Life , Blood Platelets/physiology , Traumatology , Referral and Consultation
2.
Rev. medica electron ; 43(5): 1409-1417, 2021.
Article in Spanish | LILACS | ID: biblio-1352120

ABSTRACT

RESUMEN El envejecimiento es un proceso complejo que trae consigo cambios celulares, histológicos y cutáneos. Estos últimos son una de sus manifestaciones más evidentes. El plasma rico en plaquetas es una fuente fiable de obtención de células para regenerar tejidos; por su fácil disponibilidad es un material inocuo. La bioestimulación con el mismo, por su parte, es un conjunto de procedimientos para activar las funciones anabólicas de los fibroblastos, producción de colágeno, elastina y ácido hialurónico. La tendencia al empleo de este en tratamientos antiedad es cada vez mayor. El objetivo de este trabajo fue realizar una actualización del tema, para exponer aspectos importantes sobre formas de aplicación, indicaciones, complicaciones y contraindicaciones. Existen varios métodos para la bioestimulación facial, tales como la realización de pápulas, napagge y retroinyección. Se han empleado en alopecia androgénica, areata, envejecimiento cutáneo, etc. Las complicaciones más observadas son dolor, eritema, ardor y sangrado local. Entre las contraindicaciones más comunes se observan el herpes simple recidivante, coagulopatías, tratamiento con anticoagulantes, colagenopatías y neoplasias (AU).


ABSTRACT Aging is a complex process that brings with it cellular, histological and cutaneous changes, the latter being one of its most obvious manifestations. Platelet-rich plasma is a reliable source of cells to regenerate tissues; due to its easy availability, it is a harmless material. Bio-stimulation with it is a set of procedures to activate the fibroblasts anabolic functions and the production of collagen, elastin and hyaluronic acid. The tendency to use it in anti-aging treatments increases faster and faster. The objective of this work was updating the topic to expose important aspects about application methods, indications, complications and contraindications. There are several methods of applying facial bio-stimulation such as performing papules, napagge, and retroinjection. It has been used in androgenic alopecia, alopecia areata, cutaneous ageing, etc. The most commonly found complications are pain, erythema, burning and local bleeding. The most common contraindications include recidivist herpes simplex, coagulopaties, anticoagulant treatment, collagen-related diseases and neoplasms (AU).


Subject(s)
Humans , Male , Female , Platelet-Derived Growth Factor/therapeutic use , Dermatology/methods , Platelet-Derived Growth Factor/biosynthesis , Skin Aging/drug effects , Blood-Derivative Drugs
3.
Rev. cuba. oftalmol ; 34(3): e925, 2021.
Article in Spanish | LILACS, CUMED | ID: biblio-1352031

ABSTRACT

La enfermedad de la superficie ocular incluye a un grupo de patologías con diversas etiologías, síntomas y hallazgos clínicos que comparten la producción de reacción inflamatoria y daño de esta superficie. El uso de derivados hemáticos para el tratamiento de patologías de la superficie ocular se ha incrementado en el área de la oftalmología, ya que su composición es análoga a la de la lágrima natural. Con el objetivo de mostrar la terapia celular como una nueva disciplina científica a aplicar en nuestro medio, se realizó una búsqueda automatizada sobre el tema, teniendo en cuenta las publicaciones de los últimos 5 años. Se utilizó la plataforma Infomed, cuya información fue resumida para la elaboración del informe final, donde se expone que los colirios de hemoderivados proveen estrategias de tratamiento eficaces y seguras para pacientes con afecciones oftálmicas. El colirio de plasma rico en plaquetas ofrece una opción exitosa de tratamiento en numerosas afecciones de la superficie ocular. Sin embargo, estudios adicionales son necesarios para establecer la seguridad y la eficacia de este tipo de terapias(AU)


Ocular surface diseases are a group of conditions of different etiologies, symptoms and clinical findings with the common features of developing an inflammatory reaction and damaging the ocular surface. Use of blood-derived products for the treatment of ocular surface disorders has increased in ophthalmic care, since their composition is similar to that of natural tears. With the purpose of presenting cell therapy as a new scientific discipline that could be used in our environment, an automated search was conducted about the topic which included publications from the last five years. The search was performed on the Infomed platform, and the information obtained was summarized into a final report stating that blood-derived eye drops provide effective and safe treatment strategies for patients with ophthalmic conditions. Platelet-rich plasma eye drops are a potentially successful treatment option for many ocular surface disorders. However, further studies are required to establish the safety and effectiveness of this type of therapy(AU)


Subject(s)
Humans , Ophthalmic Solutions/adverse effects , Blood-Derivative Drugs , Platelet-Rich Plasma/drug effects , Review Literature as Topic , Databases, Bibliographic , Research Report
4.
Rev. méd. Urug ; 37(3): e37302, set. 2021. tab, graf
Article in Spanish | LILACS, BNUY | ID: biblio-1341550

ABSTRACT

Resumen: Las úlceras de pie diabético (UPD) generan un alto costo para el paciente y el sistema de salud. Una deficiente vascularización, la inhibición de la generación de factores de crecimiento y la migración celular, entre otros factores, influyen en su cronicidad. Los factores de crecimiento derivados de las plaquetas (PDGF) estimulan la quimiotaxis, la neovascularización y la regeneración tisular. Objetivo: evaluar la efectividad de una formulación de plasma rico en plaquetas autólogo (PRP) como una modalidad complementaria para el tratamiento de UPD, valorando su seguridad y efectos adversos. Materiales: estudio observacional. Se aplicó PRP a seis pacientes con UPD crónicas, Wagner II, III, con una media en el retraso de la cicatrización de 94 semanas. Se administró mediante punción y como gel sobre la lesión una vez por semana hasta el cierre de la úlcera o durante12 semanas. Las UPD se analizaron con respecto al área y su progresión mediante Mobile Wound Analyzer (MOWA), software de análisis de imágenes de úlceras. El porcentaje de reducción del área se calculó entre la medición inicial y la semana 12. Como criterio de mejoría se eligió una reducción >70% de la lesión. Resultados: predominó el sexo masculino con una media de edad de 53 años ± 7,3. Luego de 12 semanas de tratamiento, se reportó una reducción media del tamaño de la úlcera de 78%, con dos cicatrizaciones completas y dos en 99%. Un paciente recibió una amputación luego de presentar infección no controlada. Conclusión: la aplicación del PRP mejoró la regeneración de tejidos, acortando la duración de la úlcera, promoviendo su curación sin efectos adversos, al tiempo que eliminó la necesidad de procedimientos de manejo en una úlcera crónica.


Summary: Diabetic foot ulcers imply high expenditure for both patients and the health system. Vascular impairment, growth factor inhibition and cell migration, among other factors, affect the chronicity of the condition. Platelet-derived growth factors (PDGFs) stimulate chemotaxis, neovascularization and tissue regeneration. Objective: to evaluate effectiveness of a formulation of autologous platelet-rich plasma to complement the treatment of diabetic foot ulcer, assessing safety and adverse effects. Material: observational study. Platelet rich plasma was applied to six patients with chronic diabetic foot ulcer Wagner II, III, with an average delayed healing of 94 weeks. PRP gel was administered by puncture technique on the lesion, once a week until the ulcer healed or for 12 weeks. Diabetic foot ulcers were analysed in regards to surface area and progression through the Mobile Wound Analyzer (MOWA) software to analyse ulcer images. The area reduction percentage was calculated comparing the initial measurement and that of week 12. The improvement criteria was defined in a reduction that is >70% of the lesion. Results: men prevailed with an average age of 53 years ± 7.3. After 12 weeks of treatment an average reduction of 78% of the size of the ulcer was reported, two complete healings and two 99% healed. One patient required amputation because of uncontrolled infection. Conclusion: the application of PRP improved tissue regeneration and shortened the duration of the ulcer, promoting healing with no adverse effects, and it likewise eliminated the need for procedures to handle chronic ulcers.


Resumo: As úlceras do pé diabético (UPD) geram alto custo para o paciente e para o sistema de saúde. A vascularização deficiente, a inibição da geração de fatores de crescimento e migração celular, entre outros fatores, influenciam sua cronicidade. Os fatores de crescimento derivados de plaquetas (PDGF) estimulam a quimiotaxia, a neovascularização e a regeneração de tecidos. Objetivo: avaliar a eficácia de uma formulação autóloga de plasma rico em plaquetas (PRP) como modalidade complementar para o tratamento da UPD, avaliando sua segurança e efeitos adversos. Materiais: estudo observacional. O PRP foi aplicado a 6 pacientes com UPD crônica, Wagner II, III, com um atraso médio na cicatrização de 94 semanas. Foi administrado por punção e como gel sobre a lesão uma vez por semana até o fechamento da úlcera ou por 12 semanas. As UPD foram analisadas em relação à área e sua progressão, utilizando o software de análise de imagem de úlcera Mobile Wound Analyzer (MOWA). A redução percentual da área foi calculada entre a medição inicial e a semana 12. Como critério de melhora, optou-se pela redução > 70% da lesão. Resultados: predominou o sexo masculino com média de idade de 53 ± 7,3 anos. Após 12 semanas de tratamento, foi relatada uma redução média no tamanho da úlcera de 78%, sendo 2 com cicatrização completa e 2 em 99%. Um paciente foi amputado após desenvolver infecção descontrolada. Conclusão: a aplicação do PRP melhorou a regeneração tecidual, encurtando o tempo de duração da úlcera, promovendo sua cicatrização sem efeitos adversos, ao mesmo tempo que eliminava a necessidade de procedimentos de manejo na úlcera crônica.


Subject(s)
Diabetic Foot/therapy , Platelet-Rich Plasma , Foot Ulcer/therapy
5.
Rev. cuba. estomatol ; 58(2): e3053, 2021. graf
Article in Spanish | LILACS, CUMED | ID: biblio-1289405

ABSTRACT

Introducción: Los desórdenes temporomandibulares son un grupo de trastornos que afectan la articulación temporomandibular y/o los tejidos musculoesqueléticos asociados. Objetivo: Determinar la efectividad de los concentrados de plaquetas en el tratamiento de los desórdenes temporomandibulares. Métodos: La búsqueda de la literatura fue realizada desde enero del 2014 hasta abril del 2019, en las bases de datos biomédicas: PubMed, Embase, SciELO, Scopus, Science Direct, Sistema de información sobre literatura gris en Europa, Literatura Latinoamericana y del Caribe en Ciencias de la Salud, Google Académico y el Registro Central de Ensayos Clínicos Cochrane. Se definieron como criterios de selección de los estudios que fueran ensayos clínicos aleatorizados, con una antigüedad máxima de cinco años, que reportarann la efectividad (reducción del dolor y aumento de apertura máxima) de los concentrados plaquetarios en el tratamiento de los desórdenes temporomandibulares. El riesgo de sesgo de los estudios fue analizado por medio del Manual Cochrane de revisiones sistemáticas de intervenciones. Resultados: La estrategia de búsqueda resultó en nueve artículos, de los cuales el 100 por ciento reportó que no había diferencia en la reducción del dolor y el aumento de apertura máxima de los concentrados plaquetarios en el tratamiento de los desórdenes temporomandibulares. Conclusiones: La literatura revisada sugiere que existe una ligera evidencia de los beneficios potenciales de las inyecciones intraarticulares de los concentrados plaquetarios en pacientes con desórdenes temporomandibulares. Sin embargo, es necesario establecer un protocolo estandarizado para la preparación y aplicación de estos concentrados(AU)


Introduction: Temporomandibular disorders are a group of dysfunctions which affect the temporomandibular joint and/or associated musculoskeletal tissues. Objective: Determine the effectiveness of platelet concentrates in the treatment of temporomandibular disorders. Methods: A bibliographic search was conducted from January 2014 to April 2019 in the biomedical databases PubMed, Embase, SciELO, Scopus, Science Direct, System for Information on Gray Literature in Europe, Latin American and Caribbean Health Sciences Literature, Google Scholar, and Cochrane Central Register of Clinical Trials. The following selection criteria were defined for the studies: randomized clinical trials published in the last five years and reporting on the effectiveness (pain reduction and maximum opening increase) of platelet concentrates in the treatment of temporomandibular disorders. Bias risk analysis was based on the Cochrane manual of systematic reviews of interventions. Results: Nine papers were retrieved, of which 100 percent reported no differences in pain reduction or maximum opening increase resulting from the use of platelet concentrates in the treatment of temporomandibular disorders. Conclusions: The literature review conducted suggests that there is slight evidence of the potential benefits of intra-articular injections of platelet concentrates in patients with temporomandibular disorders. However, a standardized protocol should be established for the preparation and application of these concentrates(AU)


Subject(s)
Humans , Temporomandibular Joint Disorders/therapy , Platelet-Rich Fibrin , Injections, Intra-Articular/methods , Review Literature as Topic , Databases, Bibliographic
6.
Arq. gastroenterol ; 58(2): 185-189, Apr.-June 2021. tab, graf
Article in English | LILACS | ID: biblio-1285312

ABSTRACT

ABSTRACT BACKGROUND: Surgical treatment of recurrent anal fistulas can lead to numerous complications, including fecal incontinence. Therefore, sphincter preserving techniques are gaining more popularity. OBJECTIVE: The aim of the study was to assess effectiveness of platelet-rich plasma (PRP) therapy in the patients with recurrent cryptoglandular anal fistulas. METHODS: A cohort of 18 patients with anal fistulas was enrolled into a preliminary and prospective trial. They were divided into two groups consisting of eight and ten patients respectively. PRP was injected locally in all patients, however in the group II it was applied after 7 days drainage of fistulas with polyurethane foam or negative pressure wound therapy. On average, three doses of PRP were administered, but with the opportunity to double the number of applications if it was clinically justified. The patients were evaluated in an out-patient department after fortnight and then in 1, 6, and 12 months following the last PRP application. RESULTS: Anal fistulas were closed in 4 (50%) patients from the group I and in 7 (70%) patients form the group II. Although, the difference between both groups was not statistically significant, PRP therapy should be preceded with fistulous tract drainage in all patients. Summarizing, that successful result was achieved in 11 (60%) patients from the entire group of 18 participants. CONCLUSION: The rate of recurrent cryptoglandular anal fistulas closure reaching 60%, after topical treatment with PRP, exceeds the results of other sphincter-saving methods of treatment. Therefore, it might become a novel method of anal fistulas therapy.


RESUMO CONTEXTO: O tratamento cirúrgico de fístulas anais recorrentes pode levar a inúmeras complicações, incluindo incontinência fecal. Portanto, as técnicas de preservação do esfíncter estão ganhando mais popularidade. OBJETIVO: O objetivo do estudo foi avaliar a eficácia da terapia de plasma rico em plaquetas (PRP) nos pacientes com fístulas anais criptoglandulares recorrentes. MÉTODOS: Uma coorte de 18 pacientes com fístulas anais foi inscrita em ensaio preliminar e prospectivo. Eles foram divididos em dois grupos compostos por 8 e 10 pacientes, respectivamente. PRP foi injetado localmente em todos os pacientes, porém no grupo II foi aplicado espuma de poliuretano ou terapia de feridas por pressão negativa após 7 dias de drenagem de fístulas. Em média, foram administradas três doses de PRP, mas com a oportunidade de dobrar o número de aplicações se fosse clinicamente justificado. Os pacientes foram avaliados em ambulatório após quinze dias e depois em 1, 6 e 12 meses após a última aplicação do PRP. RESULTADOS: As fístulas anais foram fechadas em 4 (50%) pacientes do grupo I e em 7 (70%) pacientes do grupo II. Embora a diferença entre ambos os grupos não tenha sido estatisticamente significante, a terapia PRP deve ser precedida de drenagem do trato fístulo em todos os pacientes. Resumindo, esse resultado bem-sucedido foi alcançado em 11 (60%) pacientes de todo o grupo de 18 participantes. CONCLUSÃO: A taxa de fechamento recorrente de fístulas anais criptoglandulares chegando a 60%, após tratamento tópico com PRP, excede os resultados de outros métodos de tratamento que preservam o esfíncter. Portanto, pode se tornar um novo método de terapia das fístulas anais.


Subject(s)
Humans , Rectal Fistula , Platelet-Rich Plasma , Fecal Incontinence , Anal Canal , Prospective Studies , Treatment Outcome
7.
Arq. bras. med. vet. zootec. (Online) ; 73(3): 613-621, May-June 2021. tab, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1278361

ABSTRACT

The objective in this study was to evaluate the clinic effect of applying allogenic platelet-rich plasma (PRP) heated or not, for treating cornea ulcers, including the dosage of PDGF-BB in the cornea. The ulcers were induced, standardizing the left eye from 81 rats (Ratus norvegicus, albinus variety), assigned randomly into three groups (N=27): control group (CG) which did not receive any topic treatment; heated PRP group (GA) and PRP group (GP), which received topical treatment every eight hours for five days. Each group underwent evaluation at 24 hours (M1), three days (M3) and five days (M5). The clinical exam evaluated the opacity, vascularization and corneal repair. The corneal PDGF-BB was dosed through the ELISA method. The corneal opacity was decreased in PRP-treated animals (GA and GP) and corneal repair time reduced when compared to CG at M1 and M5. Furthermore, GP showed greater vascularization at M3 compared to M1. Applied allogenic PRP eye drops, heated or not, speed up corneal healing, and reduce corneal repair time. However, the corneal PDGF concentration was not altered in any of the treatments.(AU)


Objetivou-se avaliar o efeito clínico da aplicação de plasma rico em plaquetas alogênico (PRP) aquecido ou não, no tratamento de úlceras de córnea, como a dosagem de PDGF-BB na córnea. As úlceras foram induzidas, padronizando-se o olho esquerdo de 81 ratos (Rattus norvegicus, variedade albinus), aleatoriamente, nos três grupos (N = 27): grupo controle (CG), que não recebeu nenhum tratamento tópico; grupo PRP aquecido (GA) e grupo PRP (GP), que receberam tratamento tópico a cada oito horas, durante cinco dias. Cada grupo foi subdividido em 24 horas (M1), três dias (M3) e cinco dias (M5). O exame clínico avaliou a opacidade, a vascularização e o reparo corneano. O PDGF-BB corneano foi dosado pelo método Elisa. Houve diminuição da opacidade da córnea nos animais tratados com PRP (GA e GP) e diminuição do tempo de reparo da córnea em comparação com CG, M1 e M5. Além disso, foi observada maior vascularização no GP no momento M3 em relação ao M1. A aplicação de colírios de PRP alogênico, aquecidos ou não, acelera a cicatrização da córnea, além de reduzir o tempo de reparo da córnea. No entanto, a concentração de PDGF na córnea não se alterou em nenhum dos tratamentos.(AU)


Subject(s)
Animals , Rats , Ophthalmic Solutions/therapeutic use , Platelet-Derived Growth Factor/analysis , Corneal Ulcer/chemically induced , Platelet-Rich Plasma , Enzyme-Linked Immunosorbent Assay/veterinary , Animals, Laboratory
8.
Rev. cuba. anestesiol. reanim ; 20(1): e683, ene.-abr. 2021.
Article in Spanish | LILACS, CUMED | ID: biblio-1156368

ABSTRACT

Introducción: La enfermedad degenerativa discal es una entidad frecuente y uno de los principales motivos de consulta. Genera altas tasas de discapacidad, años útiles perdidos, así como altos costos económicos por asistencia médica y grandes pérdidas monetarias. Su tratamiento es generalmente conservador, aunque en la actualidad se incluyen terapias biológicas novedosas. Objetivo: Describir las principales propiedades biológicas que hacen del plasma rico en plaquetas una terapéutica efectiva para la enfermedad degenerativa discal. Métodos: Se realizó una revisión no sistemática de la bibliografía basada en artículos que se publicaron en bases de datos indexadas en Infomed como Hinari, Ebsco, Scielo, Pubmed, Cubmed, Cocrhane, Scopus, LILACS; en idioma español, inglés y portugués, durante los últimos diez años. Desarrollo: Se expusieron características clínico epidemiológicas de la enfermedad degenerativa discal, así como las propiedades biológicas que le permiten al plasma rico en plaqueta tener una función activa en la regeneración del disco intervertebral o el retraso de la cascada de degradación de este. Se resaltan los principales estudios de acuerdo a la vía de administración del plasma rico en plaquetas y sus resultados. Conclusiones: De acuerdo con lo publicado por los autores, el plasma rico en plaquetas es una alternativa efectiva en el tratamiento de la enfermedad degenerativa discal por la producción de factores derivados de las plaquetas, que intervienen en la degeneración del disco intervertebral, siendo la vía intradiscal la que más se emplea(AU)


Introduction: Degenerative disc disease is a frequent condition and one of the main reasons to attend the consultation. It generates high rates of disability, useful years lost, as well as high economic costs for medical assistance and large monetary losses. Its treatment is generally conservative, although novel biological therapies are currently included. Objective: To describe the main biological properties that make platelet-rich plasma an effective therapy against degenerative disc disease. Methods: A nonsystematic review of the bibliography was carried out based on articles published, during the last ten years, in databases indexed in Infomed, such as Hinari, Ebsco, Scielo, Pubmed, Cubmed, Cocrhane, Scopus, and LILACS, in Spanish, English and Portuguese. Development: Clinical-epidemiological characteristics of degenerative disc disease were presented, as well as the biological properties that allow platelet-rich plasma to have an active function in the regeneration of the intervertebral disc or the delay of its degradation cascade. The main studies are highlighted, according to the route of administration of platelet-rich plasma and their results. Conclusions: According to what has been published by authors, platelet-rich plasma is an effective alternative in the treatment of degenerative disc disease, due to the production of factors derived from platelets, which intervene in the degeneration of the intervertebral disc, being the intradiscal pathway the most used(AU)


Subject(s)
Humans , Platelet-Rich Plasma/physiology , Intervertebral Disc Degeneration/therapy , Intervertebral Disc Degeneration/epidemiology , Intervertebral Disc Degeneration/surgery
9.
Rev. bras. oftalmol ; 80(2): 146-150, Mar.-Apr. 2021. graf
Article in English | LILACS | ID: biblio-1280111

ABSTRACT

ABSTRACT We propose a novel surgical technique in cases of aggressive recurrent pterygium non-subsidiary of treatment with conjunctival autografts or antimetabolites. Two presented cases were treated with surgical excision and a sutured plasma rich in growth factors membrane (mPRGF) followed by rich in growth factors (PRGF) eye drops treatment. After surgery, dexamethasone, tobramycin and PRGF eye drops were prescribed for 6 weeks. After a 12-month and 3-year post-surgical follow-up respectively, treated eyes with mPRGF did not present relapse, and visual acuity improved in both cases. No ocular complications, pain, eye discomfort nor other symptoms were observed. The combined use of PRGF eye drops and mPRGF seems an effective and safe therapy for recurrent pterygium.


RESUMO Nós propomos uma nova técnica cirúrgica em casos de pterígio agressivo recorrente não subsidiário de tratamento com autoenxertos conjuntivais ou antimetabólitos. Dois casos foram tratados com excisão cirúrgica e um plasma suturado rico em membrana de fatores de crescimento (mPRGF), seguido de tratamento com colírios ricos em fatores de crescimento (PRGF). Após a cirurgia, foram prescritos colírios de dexametasona, tobramicina e PRGF por 6 semanas. Após 12 meses e 3 anos de acompanhamento pós-cirúrgico respectivamente, os olhos tratados com mPRGF não apresentaram recidiva e a acuidade visual melhorou nos dois casos. Não foram observadas complicações oculares, dor, desconforto ocular ou outros sintomas. O uso combinado de colírios de PRGF e mPRGF parece uma terapia eficaz e segura para o pterígio recorrente.


Subject(s)
Humans , Male , Middle Aged , Aged , Pterygium/surgery , Platelet-Rich Plasma , Platelet-Rich Fibrin , Ophthalmic Solutions , Recurrence , Reoperation , Ophthalmologic Surgical Procedures/methods , Biological Dressings , Fibrin/therapeutic use , Platelet Activation , Tissue Transplantation/methods , Tissue Engineering
10.
Article in English | WPRIM | ID: wpr-873901

ABSTRACT

Objective: This study aimed to examine differences in platelet-rich plasma (PRP) soak-loaded volumes of β-tricalcium phosphate (β-TCP) with or without a unidirectional porous structure.Materials and Methods: Leukocyte-rich PRP was extracted from 15 healthy volunteers by centrifugation. Two types of artificial bones were soaked for either ten seconds or ten minutes. The volume ratios of PRP soak-loaded onto the artificial bone and soaked area ratios were evaluated. Statistical analyses were performed using the Tukey-Kramer HSD test and the Games-Howell method. A P-value of <0.05 was considered statistically significant.Results: Regardless of the soaking time, the PRP soak-loaded volume ratio and soaked area ratio were significantly higher in the unidirectional porous β-TCP (UDPTCP) group than in the spherical porous β-TCP (SPTCP) group.Conclusion: PRP can be soak-loaded faster and in larger amounts onto UDPTCP compared to SPTCP. Understanding the basic biology of β-TCP soak-loaded with PRP can help develop more novel and effective β-TCP treatments for orthopedic surgery.

11.
Braz. j. med. biol. res ; 54(2): e9944, 2021. tab, graf
Article in English | ColecionaSUS, LILACS, ColecionaSUS | ID: biblio-1142581

ABSTRACT

The aim of this study was to inhibit adipogenic differentiation by transfecting two growth factors, platelet-derived growth factor (PDGF-BB) and bone morphogenic protein 2 (BMP-2), into modified rat bone marrow mesenchymal stem cells (rBMSCs) and then compounded with platelet-rich plasma (PRP). To achieve rBMSCs, the osteoporosis model of rats was established, and then the rBMSCs from the rats were isolated and identified. Co-transfection of rBMSCs with PDGF-BB-GFP and BMP-2 and detection of PDGF-BB/BMP-2 expression in transfected BMSCs was assessed by qRT-PCR and western blot, respectively. Moreover, the effect of the two growth factors transfection of rBMSCs on adipogenic differentiation was evaluated by oil red O staining and western blot, respectively. Finally, construction of the two growth factors transfection of rBMSCs compounded with PRP and detection of adipogenic differentiation were assessed by oil red O staining, CCK-8, and western blot, respectively. In vitro studies revealed that the two growth factors transfection of rBMSCs compounded with PRP promoted cell viability and inhibited adipogenic differentiation and could be promising for inhibiting adipogenic differentiation.


Subject(s)
Animals , Rats , Cell Differentiation , Adipose Tissue/cytology , Platelet-Rich Plasma , Bone Morphogenetic Protein 2/genetics , Mesenchymal Stem Cells/cytology , Becaplermin/genetics , Transfection , Cells, Cultured
12.
Pesqui. vet. bras ; 41: e06999, 2021. tab, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1351280

ABSTRACT

Platelet-rich plasma (PRP) has been considered a promising therapeutic alternative, since platelets are rich in growth factors that are used in the Regenerative Medicine field. However, fresh PRP cannot be stored for long periods. This study aimed to develop a protocol for obtaining lyophilized canine PRP capable of maintaining viability after its reconstitution. For that purpose, canine PRP extraction and lyophilization protocols were initially tested. Subsequently, assays were carried out to quantify the growth factors VEGF and TGF-β, before and after the lyophilization process, gelation test and the three-dimensional gel structure analysis of the reconstituted lyophilized PRP by electron microscopy, as well as in vitro cell proliferation test in lyophilized PRP gel. Additionally, the immunogenicity test was performed, using allogeneic samples of lyophilized PRP. The results showed that the lyophilized PRP had adequate therapeutic concentrations of growth factors VEGF and TGF-β (9.1pg/mL and 6161.6pg/mL, respectively). The reconstituted PRP gel after lyophilization showed an in vitro durability of 10 days. Its electron microscopy structure was similar to that of fresh PRP. In the cell proliferation test, an intense division process was verified in mesenchymal stem cells (MSCs) through the three-dimensional mesh structure of the lyophilized PRP gel. The immunogenicity test showed no evidence of an immune reaction. The findings were promising, suggesting the possibility of having a lyophilized canine PRP that can be marketed. New in vivo and in vitro studies must be carried out for therapeutic confirmation.(AU)


O plasma rico em plaquetas (PRP) é uma alternativa terapêutica promissora, pois as plaquetas são ricas em fatores de crescimento com ação na regeneração de tecidos. No entanto, o PRP fresco não pode ser armazenado por longos períodos. Esse trabalho teve como objetivo desenvolver um protocolo de obtenção de PRP liofilizado canino capaz de manter a viabilidade pós reconstituição. Portanto, foram testados diversos protocolos de extração e liofilização. Para validação do PRP canino liofilizado foi analisada a concentração dos fatores de crescimento VEGF e TGF-β antes e após o processo de liofilização, a estrutura tridimensional do PRP liofilizado reconstituído em forma de gel por microscopia eletrônica e seu efeito in vitro na proliferação de células-tronco mesenquimais. Os resultados demonstraram que o PRP liofilizado apresentou concentrações terapêuticas adequadas dos fatores de crescimento VEGF e TGF- β (9,1pg/ml e 6161,6pg/ml, respectivamente). O gel de PRP reconstituído após liofilização apresentou uma durabilidade in vitro de 10 dias, sua estrutura tridimensional mostrou-se semelhante ao PRP fresco e proporcionou intensa proliferação de células-tronco mesenquimais durante o cultivo. O teste de imunogenicidade não demonstrou evidências de reação imune. Os achados foram promissores, sugerindo a possibilidade de uso de PRP canino liofilizado para o mercado. Novos estudos in vivo e in vitro deverão ser conduzidos para comprovação terapêutica.(AU)


Subject(s)
Animals , Dogs , In Vitro Techniques , Platelet-Rich Plasma , Mesenchymal Stem Cells , Freeze Drying , Therapeutics , Dogs
13.
Acta cir. bras ; 36(12): e361203, 2021. tab, graf
Article in English | LILACS-Express | MEDLINE, LILACSEXPRESS, LILACS, VETINDEX | ID: biblio-1355569

ABSTRACT

ABSTRACT Purpose: To evaluate whether using platelet-rich plasma (PRP) in the graft recipient bed after the resection of a neoplasia can influence its recurrence because this product stimulates angiogenesis, mitogenesis and chemotaxis. Methods: A study with 30 rats Wistar (Rattus norvegicus albinus), which were separated into group A (induction of carcinogenesis, PRP in the postoperative period) and group B (induction of carcinogenesis, absence of PRP in the postoperative period), with 15 animals in each. Carcinogenesis was induced on the skin of the animals' chest by the topical application of 0.5% dimethylbenzantracene (DMBA) diluted in acetone. After surgical resection of the induced neoplasia, PRP was used to stimulate angiogenesis before surgical wound synthesis. Data on the control and experimental groups and macroscopic and microscopic variables were evaluated using analysis of variance and the Tukey's test (5%). Results: It was possible to determine that the use of PRP is good in reconstructive surgeries, but it is contraindicated in patients during tumor resection, as it can cause changes in the surgical bed, in addition to stimulating recurrences and metastases. Conclusions: PRP may interact with tumour cells that were in the recipient site of the surgical wound during the resection of a neoplasia, and a local recurrence process can be triggered by applying this product.

14.
Chinese Journal of Trauma ; (12): 295-300, 2021.
Article in Chinese | WPRIM | ID: wpr-909868

ABSTRACT

Infectious bone defect is a serious complication after high-energy trauma, osteomyelitis and infection of implants. It has become one of the most difficult diseases in orthopedics. The formation of biofilm is an important factor of infectious bone defect. With the wide application and success of platelet-rich plasma (PRP) in orthopedics and other fields, PRP can be used as a new biomaterial to load antibiotics and become a new treatment method for infectious diseases, including infectious bone defects. The authors discuss the characteristics of PRP-loaded antibiotics combined with Masquelet technique in treatment of infectious bone defect from the aspects of advantages, operation points, indications and operation methods, so as to provide references for treatment of infectious bone defect in clinic.

16.
Article in Chinese | WPRIM | ID: wpr-921910

ABSTRACT

OBJECTIVE@#To evaluate clinical efficacy of arthroscopic with platelet-rich plasma (PRP) in treating meniscus injury.@*METHODS@#From January 2015 to December 2019, clinical control study on repair meniscus injury by arthroscopic with PRP between arthroscopic were searched by PubMed, Science Direct, Cochrane library, Chinese Journal Full-text Database, Wanfang and VIP database. Literature screening, data extraction and quality evaluation according to inclusion and exclusion criteria. Visual analogue scale(VAS) of knee joint and Lysholm score at 1, 6 and 12 months after opertaion between two groups were compared, and Western Ontario and McMaster University Osteoarthritis Index (WOMAC) at 3, 6 and 12 months after opertaion between twogroups were also compared.@*RESULTS@#Totally 9 literatures and 329 patients with meniscal injuries were screened, include 146 patients treated by arthroscopic with PRP and 183 patients treated by arthroscopic. There were no statistical differences in VAS between two groups at 1, 6 and 12 months after opertaion. There were differences in Lysholm score at 1 and 6 months after operation between two groups [@*CONCLUSION@#Arthroscopic with PRP for repair meniscus injury has short term efficacy of knee function and delay arthritis, while has similar effect in long term clinical efficacy and relieve pain.


Subject(s)
Arthroscopy , Humans , Knee Joint , Meniscus , Osteoarthritis, Knee , Platelet-Rich Plasma , Tibial Meniscus Injuries/surgery , Treatment Outcome
17.
Article in English | WPRIM | ID: wpr-920842

ABSTRACT

@#Introduction: Both short and long PFNA are employed to treat intertrochanteric fractures. Controversy exists in the choice between the two nails as each implant has specific characteristics and theoretical advantages. This retrospective study seeks to examine the operative complication rates and clinical outcomes of short versus long (Proximal Femoral Nail Antirotation) PFNA in the treatment of intertrochanteric fractures. Materials and methods: Between July 2011 and February 2015, 155 patients underwent PFNA insertion. The decision on whether to use a short or long PFNA nail, locked or unlocked, was determined by the attending operating surgeon. Visual Analogue Pain Score (VAS) Harris Hip Scores (HHS), Short-form 36 Health Questionnaire (SF-36) and Parker Mobility Scores (PMS) were collected at six weeks, six months and one year post-operatively. Results: A total of 137 (88.4%) patients were successfully followed-up. Forty-two (30.7%) patients received a short PFNA. The patients were similar in baseline characteristics of age, gender, and comorbidities. Operative time was significantly longer in the short PFNA group (62 ±17 mins) versus the long PFNA group (56±17). While the patients in both groups achieved improvement in all outcome measures, there was no significant difference between the groups in terms of HHS (61.0 ±16.0 vs 63.0 ±16.8, p=0.443), PMS (2.3±1.5 vs 2.7±2.1, p=0.545) and VAS (1.7±2.9 vs 1.8 ±2.2 p=0.454). There were 3 (7.1%) and 7 (7.4%) complications in the short versus long PFNA group, respectively. Conclusion: Both short and long PFNA had similar clinical outcomes and complication rates in the treatment of intertrochanteric fractures in an Asian population.

18.
Article in Chinese | WPRIM | ID: wpr-848020

ABSTRACT

BACKGROUND: Studies have shown that platelet-rich plasma has a strong role in promoting chondrocyte repair and proliferation. OBJECTIVE: To investigate the efficacy of platelet-rich plasma in chondrocyte repair and synovial inflammation inhibition in osteoarthritis. METHODS: The blood samples were extracted from the central artery of the ear in 40 New Zealand white rabbits and platelet-rich plasma was prepared by the method of Hokugo. The platelet, platelet-derived growth factor, transforming growth factor-3, vascular endothelial growth factor in the peripheral blood and platelet-rich plasma were detected simultaneously. Animal models were made by resection of anterior cruciate ligament and divided into experimental group and control group. In the experimental group, 0.3 mL of platelet-rich plasma was injected once a week for 10 weeks; in the control group, 0.3 mL of sterile saline was injected once a week for 10 weeks. Gross observation and histological observation of knee joints were performed; articular cartilage type II collagen and matrix metalloproteinase 13 levels were measured; and Mankin score of the cartilage tissue was performed at 2, 4, 6, 8, and 10 weeks after injection. The study protocol was approved by the Animal Experiment Ethics Committee of Chongqing Medical University. RESULTS AND CONCLUSION: (1) The concentrations of platelet, platelet-derived growth factor, transforming growth factor-3, vascular endothelial growth factor in platelet-rich plasma were 5.5, 4.8, 7.7, and 6.2 times those of the peripheral blood, respectively. And there were significant differences between the platelet-rich plasma and peripheral blood (P<0.05). (2) From the 6th week, the Mankin score of the experimental group was significantly lower than that of the control group (P<0.05). (3) The expression of type II collagen protein in the experimental group was significantly higher than that in the control group at the 4th, 6th, 8th and 10th weeks after injection (P<0.05). The expression of matrix metalloproteinase-13 was significantly lower than that in the control group at the 2nd, 4th, 6th, 8th and 10th week (P<0.05). All these findings indicate that intra-articular injection of platelet-rich plasma can inhibit the development of osteoarthritis by alleviating synovial inflammation and delaying or blocking the damage of chondrocytes.

19.
Article in Chinese | WPRIM | ID: wpr-847832

ABSTRACT

BACKGROUND: At present, platelet-rich plasma has been used in bums, plastic surgery, surgery and orthopedics. However, due to the different standards for platelet-rich plasma preparation, the results of research on bums are controversial. OBJECTIVE: To systematically evaluate the effectiveness of platelet-rich plasma in the repair of burn wounds. METHODS: A computer-based online search of CNKI, VIP, WanFang, CBMdisc, PubMed, Embase, CochraneLibrary, Web of Science databases to retrieve randomized controlled trials regarding platelet-rich plasma repair of bum wounds. In the treatment group, platelet-rich plasma therapy or platelet-rich plasma therapy combined with conventional or other therapies were used. In the control group, conventional therapy, other therapies, or placebo treatment were used. After literature screening, information extraction and quality evaluation, Review Manager 5.3 software was used for meta analysis. RESULTS AND CONCLUSION: Eleven articles were included, involving 837 patients consisting of 420 patients in the treatment group and 417 patients in the control group. Meta analysis results revealed that the wound healing rate in the treatment group was significantly higher than that in the control group [OR=12.61, 95%Cl (6.93,18.29), P<0.000 1], Wound healing time in the treatment group was significantly shorter than that in the control group [OR=-4.64, 95%Cl (-6.21, -3.06), P<0.000 01]. The number of layers of gauze soaked by dressing [OR=-4.01, 95%Cl (-4.91, -3.11), P<0.000 01], the times of changing inner gauze [OR=-3.79, 95%Cl (-5.33, -2.26), P<0.000 01, and the times of dressing change on wound surface [OR=-3.41, 95%Cl (-5.93, -0.88), P=0.008] in the treatment group were significantly less than those in the control group. The positive rate of bacterial culture in wound [OR=0.46, 95%Cl (0.22, 0.94), P=0.03] and the incidence of inflammatory reaction in wound [OR=0.30, 95%Cl (0.17, 0.56), P=0.000 1] in the treatment group were significantly lower than those in the control group. The results suggested that platelet-rich plasma can increase the wound healing rate, shorten wound healing time, reduce the number of layers of gauze soaked by dressing, the times of changing inner gauze, and the times of dressing change on wound surface, and lower the positive rate of bacterial culture and the incidence of inflammatory reaction in wound.

20.
Article in Chinese | WPRIM | ID: wpr-847220

ABSTRACT

BACKGROUND: Autologous blood concentrates are widely applied in clinical practice. They are easy to prepare, and can promote the regeneration of soft and hard tissues. Their application prospects can be broad in periodontal treatment, oral implant therapy and many other fields. OBJECTIVE: To review the development, biological characteristics and clinical application of autohemoconcentrate. METHODS: The first author searched the related literature published on PubMed, CNKI and Wanfang databases from 2004 to 2019. The key words were “autologous blood concentration, platelet-rich plasma, platelet-rich fibrin” in English, and “blood concentration, platelet-rich plasma, platelet-rich fibrin” in Chinese. The titles and abstracts were selected, and the full text was consulted. Finally, 59 articles were retained for careful reading and analysis. RESULTS AND CONCLUSION: Containing huge amount of platelets and leukocytes, autologous blood concentrate could produce a highly positive effect on the regeneration of soft and hard tissues and the reduction of postoperative inflammatory response. When it is in use alone or in combination with other bone transplantation materials, the effect of bone augmentation is positive. However, the degradation rate is high. How to control its absorption rate requires further study.

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