ABSTRACT
Objective To establish a method for comprehensive quality evaluation of Tongjing ointment by multi-component quantification combined with chemometrics and grey correlation analysis(GRA).Methods With curcumin as the internal reference substance,HPLC-QAMS method was used to simultaneously determine the contents of limonin,evodiamine,rutaecarpine,bisdemethoxycurcumin,demethoxycurcumin,curcumin,6-gingerol,8-gingerol,10-gingerol,tetrahydropalmatine,dehydrocorydalin,dorydaline in Tongjing ointment.The quality of Tongjing ointment was evaluated by cluster analysis,principal component analysis,orthogonal partial least square discriminant analysis and GRA.Results The determination of 12 components manifested a good linear relationship in the range of mass concentration(r≥0.999 1).The average recovery was between 96.58％and 100.19％(RSD＜2.0％,n=9).There were no significant difference between the measured value of external standard method and the calculated value of HPLC-QAMS(P＞0.05).Tongjing ointment samples were classified into three eategories by chemometrics and it showed that curcumin,6-gingerol,bisdemethoxycurcumin,demethoxycurcumin,limonin and tetrahydropalmatine were the main potential markers affecting the quality of Tongjing ointment.GRA showed that the relative correlation degree was in the range of 0.317 3-0.624 8,and there were some differences in the quality of Tongjing ointment.Conclusion The established method can comprehensively evaluate the quality of Tongjing ointment.
ABSTRACT
OBJECTIVE:To explore the regularity of relative correction factor (RCF) in multi-components by single marker (QAMS). METHODS:With the source of CNKI,literature retrieval was used to collect the relevant documents of multi-compo-nents by single marker (QAMS) and extract the calculated values by quantitative analysis of multi-components by single marker QAMS and measured values by external standard method (ESM),SPSS 19.0 software was used to calculate the percentage value (RSD,%) of relative standard deviation between calculated values and measured values,and the correlation between RSD and RCF was analyzed by drawing a scatter plot. RESULTS:Most of RCF values distributed in the range of >0.62-1.53,accounting for 76.7%to all data. Calculated values and measured values showed high similarity in this range and the RSD<3.0%data account-ed for 88.8% to all data,which had good credibility. Meanwhile,the similarity of calculated values and measured values was influ-enced by the differences of structure and physicochemical property of parent nucleus between other components under measured and internal references. The high degree of similarity between calculated values and measured values were decisive by very similar struc-tures of parent nucleus;the low degree was decisive by discrepant structures;and the low degree was also decisive by very similar structures and discrepant physicochemical property of parent nucleus. CONCLUSIONS:RCF will affect the credibility of content de-termination results for components under measured in QAMS,it is necessary to establish database of RCF,RSD and related param-eters.