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Article in Chinese | WPRIM | ID: wpr-463332


Objective To find lymphoma cell markers characteristic of gas for breath monitoring application and provide experimental evidence for the early diagnosis of lymphoma patients,assessing solid phase micro extraction-gas chromatography / mass spectrometry( SPME-GC / MS)is applied to the lymphatic the feasibility of detecting tumor cells in the headspace. To investigate the value of VOCs in the diagnosis of hematologic malignancies. Meth-ods The air samples from the headspace of non-Hodgkin lymphoma cell JEOK,the human lymphoidcell lines and culture medium were collected by syringes,and then determined by means of SPME-GC / MS in order to have a bet-ter understanding of the concentration distributions and changes of VOCs in JEOK cells headspace. Using Mann-Whitney U test,we found the characteristic volatile markers of non-Hodgkin′s lymphoma cells. Results SPME-GC /MS for JEOK cell lines,human lymphocytecell line and a blank incubation were detected headspace analysis,found JEOK cell culture headspace can be detected dimethyl,sulfide,toluene,o-xylene,1,3-Di-tert-butylbenzene,aceto-pheno-ne,dodecane,representing human lymphocytes increased;and alcohol,benzaldehyde,hexanal is signifi-cantly reduced. Conclusion Non-Hodgkin′s lymphoma cell lines can cause flasks JEOK headspace change in the composition of VOCs,increased alkanes and aromatic compounds,aldehydes and reducing alcohol content,these substances may be used as acharacteristic of lymphoma cells markers;SPME-GC / MS as a trace substance detection method can be used to JEOK cell lines VOCs bottle headspace detection training.