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Duazary ; 16(3): 7-24, 2019. tab
Article in English | LILACS, COLNAL | ID: biblio-1052201


This study focuses on the knowledge, perceptions and practices of 171 university students regarding Zika virus and its vector in Santa Marta (Colombia) in 2016. A survey was conducted, and the answers about causative agent and mode of transmission were classified into three levels of knowledge. Altogether, 32.1% of the students stated that they had suffered from Zika. A total of 60% stated that they knew what the disease was; however, only 29.2% knew what the causative agent was, and 45.6% knew the mode of transmission. Regarding the level of knowledge, only 14.6% knew the causative agent and the mode of transmission (Level 2). In general, the students recognize the symptoms of Zika virus, and 53.8% of them consider Zika to be very serious. More than half of them believe that they, the community, and the government are responsible for controlling the vector. Even though more than half the students know the most important strategies to control the vector, they do not apply them; the reasons for this might be their everyday habits, the lack of organization in their communities, a deficient public health system, and climate change. It is recommended to implement permanent strategies for vector control that take into account the sociocultural characteristics of at-risk populations.

Este estudio analiza el nivel de conocimiento, percepciones y prácticas de 171 estudiantes universitarios sobre el virus del Zika y su vector en Santa Marta (Colombia) en 2016. Se aplicó una encuesta y las respuestas sobre el agente causativo y el modo de transmisión se clasificaron en tres niveles de conocimiento. Se encontró que el 32,1% de los estudiantes manifiestan haber sufrido Zika. Un 60% afirmó que conocían la enfermedad, sin embargo, solo el 29,2% conocía el agente causal y el 45,6% el modo de transmisión. Sólo el 14,6% conocía el agente causal y el modo de transmisión (Nivel 2). En general, los estudiantes reconocen los síntomas del Zika. Para el 53,8% es una enfermedad muy grave y más de la mitad considera que ellos, la comunidad y el gobierno son responsables de controlar el vector. Aunque más de la mitad de los estudiantes conocen las estrategias más importantes para controlar el vector, no las practican; esto puede explicarse debido a sus prácticas cotidianas, falta de organización comunitaria, deficiencia en el sistema de salud pública y el cambio climático. Se recomienda implementar estrategias permanentes de control de vectores que consideren las características socioculturales de las poblaciones en riesgo.

Zika Virus
Article in English | WPRIM | ID: wpr-764240


Zika virus (ZIKV) is one of the pathogens which is transmitted world widely, but there are no effective drugs and vaccines. Whole genome sequencing (WGS) of viruses could be applied to viral pathogen characterization, diagnosis, molecular surveillance, and even finding novel pathogens. We established an improved method using direct RNA sequencing with Nanopore technology to obtain WGS of ZIKV, after adding poly (A) tails to viral RNA. This established method does not require specific primers, complimentary DNA (cDNA) synthesis, and polymerase chain reaction (PCR)-based enrichment, resulting in the reduction of biases as well as of the ability to find novel RNA viruses. Nanopore technology also allows to read long sequences. It makes WGS easier and faster with long-read assembly. In this study, we obtained WGS of two strains of ZIKV following the established protocol. The sequenced reads resulted in 99% and 100% genome coverage with 63.5X and 21,136X, for the ZIKV PRVABC59 and MR 766 strains, respectively. The sequence identities of the ZIKV PRVABC59 and MR 766 strains for each reference genomes were 98.76% and 99.72%, respectively. We also found that the maximum length of reads was 10,311 bp which is almost the whole genome size of ZIKV. These long-reads could make overall structure of whole genome easily, and WGS faster and easier. The protocol in this study could provide rapid and efficient WGS that could be applied to study the biology of RNA viruses including identification, characterization, and global surveillance.

Bias , Biology , Diagnosis , DNA , Genome , Genome Size , Methods , Nanopores , Polymerase Chain Reaction , RNA Viruses , RNA , RNA, Viral , Sequence Analysis, RNA , Tail , Vaccines , Zika Virus
Article in Chinese | WPRIM | ID: wpr-693365


Zika virus(ZIKV)is an arbovirus transmitted mainly through Aedes aegypti,which is associated with adult Guil-lain-Barré syndrome and microcephaly,as well as other neurological deficits in the neonate.On Feb.1,2016,the outbreak of ZIKV disease was regarded as an international public health emergency by WHO,and anti-ZIKV has become a worldwide medical chal-lenge.There are currently no vaccines and specific antiviral therapeutics available for ZIKV.In this paper,the possible pathogenesis of ZIKV and the new progress in the treatment drugs are reviewed.