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1.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 236-245, 2024.
Article in Chinese | WPRIM | ID: wpr-1006289

ABSTRACT

Alzheimer's disease (AD) is one of the most common diseases in the elderly population. Its etiology involves multiple pathogenic factors and pathological links such as abnormal deposition of β amyloid protein (Aβ), hyperphosphorylation of Tau protein, abnormalities of the cholinergic system, oxidative stress, and inflammatory response. However, its specific pathogenesis has not been clarified, and no specific therapeutic drugs have been found. In recent years, more and more studies have paid attention to the potential of chemical components of traditional Chinese medicine (TCM) in the treatment of AD. However, the diversity and complexity of the chemical components of TCM may have a positive impact on multiple pathological links of AD. Researchers have isolated many active components from TCMs, and the effects of treating AD have been confirmed by modern pharmacological studies. Through literature analysis, this article found that the main chemical components of TCM with anti-AD effects were saponins (31%), flavonoids (24%), polysaccharides (20%), lactones (8%), alkaloids (7%), phenols (3%), and other compounds (7%). Among them, ginsenoside, notoginsenoside, epimedium flavones, puerarin, baicalein, schisandra polysaccharide, angelica polysaccharide, ganoderma lucidum polysaccharide, pachyman, huperzine A, berberine, andrographolide, curcumin, emodin, and gastrodin have been extensively studied in terms of their anti-AD effects, and their mechanisms of pharmacological action have been involved in many aspects of AD pathogenesis. This article reviews the anti-AD activities and possible mechanisms of chemical components of TCM, so as to provide a reference for the development of new drugs for the prevention and treatment of AD.

2.
Arq. ciências saúde UNIPAR ; 27(10): 5537-5548, 2023.
Article in Portuguese | LILACS-Express | LILACS | ID: biblio-1511661

ABSTRACT

A Aloysia gratíssima, popularmente conhecida como alfazema-do-brasil é uma importante planta medicinal, sendo o óleo essencial rico em terpenos. Determinar o horário de colheita que resulte em maior rendimento, teor de óleo essencial e compostos químicos, são informações importantes para toda cadeia produtiva de plantas medicinais. Neste sentido, o objetivo do trabalho foi analisar o rendimento, teor e composição química do óleo essencial de A. gratíssima, em diferentes horários de colheita. O experimento foi realizado na Universidade Tecnológica Federal do Paraná - Campus Dois Vizinhos, nos anos de 2018 e 2019. Os tratamentos foram diferentes horários de colheita (9:00, 11:00, 15:00 e 17:00 horas) de massa fresca de A. gratíssima. O óleo essencial foi obtido pelo método de hidrodestilação e a composição química determinada por cromatografia gasosa-espectrometria de massa. Os melhores resultados de teor (1,18 e 0,55%) e rendimento de óleo essencial (0,71 e 0,33 g planta-1) foram obtidos nos horários de colheita das 9:00 e 11:00 horas, respectivamente, quando as plantas estavam no estádio de floração, e também as condições climáticas favoráveis, como temperaturas amenas. A cromatografia gasosa-espectrometria de massa revelou que os principais componentes dos óleos essenciais foram classificados como terpenos. Eucaliptol (39,30%) foi o componente majoritário do óleo essencial no horário de colheita das 9:00 horas. Os resultados desse estudo podem contribuir para otimizar o período de colheita de A. gratíssima, quanto à quantidade e qualidade dos óleos essenciais para indústria de produtos farmacêuticos e cosméticos.


Aloysia gratissima, popularly known as Brazilian lavender, is an important medicinal plant, the essential oil being rich in terpenes. Determining the harvest time that results in greater yield, essential oil content and chemical compounds, are important information for the entire production chain of medicinal plants In this sense, the objective of this work was to analyze the yield, content and chemical composition of the essential oil of A. gratíssima, at different harvest times. The experiment was carried out at the Universidade Tecnológica Federal do Paraná - Campus Dois Vizinhos, in the years 2018 and 2019. The treatments were different harvest times (9:00 am, 11:00 am, 3:00 pm and 5:00 pm) of fresh mass by A. gratissima. The essential oil was obtained by the hydrodistillation method and the chemical composition determined by gas chromatography-mass spectrometry. The best results for essential oil content (1.18 and 0.55%) and yield (0.71 and 0.33 g plant-1) were obtained at 9:00 am and 11:00 am, respectively, when the plants were in the flowering stage, and also favorable climatic conditions, such as mild temperatures. Gas chromatography-mass spectrometry revealed that the main components of essential oils were as terpenes. Eucalyptol (39.30%) was the major component of the essential oil at the 9:00 am harvest time. The results of this study may contribute to optimizing the period of harvesting A. gratissima, regarding the quantity and quality of essential oils for the pharmaceutical and cosmetics industry.


Aloysia gratissima, conocida popularmente como lavanda brasileña, es una importante planta medicinal, siendo el aceite esencial rico en terpenos. Determinar el tiempo de cosecha que resulte en mayor rendimiento, contenido de aceites esenciales y compuestos químicos, son información importante para toda la cadena productiva de las plantas medicinales. En ese sentido, el objetivo de este trabajo fue analizar el rendimiento, contenido y composición química del aceite esencial de A. gratíssima, en diferentes épocas de cosecha. El experimento se realizó en la Universidad Tecnológica Federal de Paraná - Campus Dois Vizinhos, en los años 2018 y 2019. Los tratamientos fueron diferentes tiempos de cosecha (9:00 am, 11:00 am, 3:00 pm y 5:00 pm ) de masa fresca por A. gratissima. El aceite esencial se obtuvo por el método de hidrodestilación y la composición química determinada por cromatografía de gases-espectrometría de masas. Los mejores resultados para contenido de aceite esencial (1.18 y 0.55%) y rendimiento (0.71 y 0.33 g planta-1) se obtuvieron a las 9:00 am y 11:00 am, respectivamente, cuando las plantas se encontraban en etapa de floración, y también condiciones climáticas favorables, como temperaturas suaves. La cromatografía de gases-espectrometría de masas reveló que los principales componentes de los aceites esenciales se clasificaron como terpenos. El eucaliptol (39,30%) fue el componente mayoritario del aceite esencial a las 9:00 a. m. del tiempo de cosecha. Los resultados de este estudio pueden contribuir a optimizar el período de cosecha de A. gratissima, en cuanto a la cantidad y calidad de los aceites esenciales para la industria farmacéutica y cosmética.

3.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 257-264, 2023.
Article in Chinese | WPRIM | ID: wpr-988204

ABSTRACT

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by mechanisms including excessive deposition of β amyloid (Aβ), neuroinflammatory responses, and hyperphosphorylation of microtubule-associated protein (Tau protein). Currently, there are no effective clinical treatments available for AD. Traditional Chinese herbal drugs have gained attention for their potential to exert anti-AD effects through multi-component, multi-target approaches. As a traditional Chinese herbal drug with over 600 years of clinical use, Geranii Herba has substantial medicinal potential and wide application prospects. Geranium medicinal plants contain chemical components such as tannins, flavonoids, organic acids, and volatile oils. Research has indicated that various tannin compounds found in Geranii Herba possess pharmacological activities like enhancing learning and memory abilities, and improving cognitive function. These effects are linked to mechanisms involving anti-Aβ effects, Tau protein regulation, antioxidation, anti-inflammation, and inhibition of acetylcholinesterase activity. These compounds can act through multiple pathways and targets to inhibit neurodegenerative changes in neurons, thus effectively preventing and treating neurodegenerative diseases like AD. Based on relevant literature, this study focused on reviewing various tannin components in Geranium plants and their role in preventing and treating AD and identified potential drug components for treating neurodegenerative diseases such as AD by exploring the tannin components and their mechanisms in Geranium plants, thereby providing a theoretical foundation and research direction for further development and clinical application.

4.
China Journal of Chinese Materia Medica ; (24): 1249-1263, 2023.
Article in Chinese | WPRIM | ID: wpr-970596

ABSTRACT

The chemical components of Huanglian Decoction were identified by ultra-performance liquid chromatography-quadrupole-time-of-flight-tandem mass spectrometry(UPLC-Q-TOF-MS/MS) technology. The gradient elution was conducted in Agilent ZORBAX Extend-C_(18) column(2.1 mm×100 mm, 1.8 μm) with the mobile phase of 0.1% formic acid aqueous solution(A)-acetonitrile(B) at a flow rate of 0.3 mL·min~(-1) and the column temperature of 35 ℃. The MS adopted the positive and negative ion mode of electrospray ionization(ESI), and the MS data were collected under the scanning range of m/z 100-1 500. Through high-resolution MS data analysis, combined with literature comparison and confirmation of reference substances, this paper identified 134 chemical components in Huanglian Decoction, including 12 alkaloids, 23 flavonoids, 22 terpenes and saponins, 12 phenols, 7 coumarins, 12 amino acids, 23 organic acids, and 23 other compounds, and the medicinal sources of the compounds were ascribed. Based on the previous studies, 7 components were selected as the index components. Combined with the network pharmacology research and analysis me-thods, the protein and protein interaction(PPI) network information of the intersection targets was obtained through the STRING 11.0 database, and 20 core targets of efficacy were screened out. In this study, UPLC-Q-TOF-MS/MS technology was successfully used to comprehensively analyze and identify the chemical components of Huanglian Decoction, and the core targets of its efficacy were discussed in combination with network pharmacology, which laid the foundation for clarifying the material basis and quality control of Huanglian Decoction.


Subject(s)
Tandem Mass Spectrometry , Network Pharmacology , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal/chemistry , Technology
5.
Digital Chinese Medicine ; (4): 426-437, 2023.
Article in English | WPRIM | ID: wpr-1011486

ABSTRACT

Objective @#To identify the main components in the extracts of different parts of Juandan Baihe (Lilium lancifolium) by ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technology and investigate their hypoglycemic activities.@*Methods@#The MS fragmentation pathways of the main types of compounds in Juandan Baihe (Lilium lancifolium) were studied, and the main components in the extracts were systematically identified using MS fragmentation pathways combined with MS mining technology. Based on the hyperglycemia male mouse model [specific pathogen free (SPF)-grade Kunming mice] induced by streptozotocin (intragastric administration of 80 mg/kg for 3 d), the hypoglycemic effects of extracts of Juandan Baihe (Lilium lancifolium) roots, stems, corms, leaves, and flowers were evaluated by measuring the changes of blood glucose, daily water consumption, daily food intake, and body weight.@*Result@#The MS fragmentation pathways of regalosides, dioscins, phenylpropanoids, flavonoids, and chlorogenic acids in Juandan Baihe (Lilium lancifolium) were clarified, and a mining method for compounds in this plant was constructed. A total of 58 compounds, including 6 chlorogenic acids, 14 regalosides, 13 phenylpropanoids, 5 flavonoids, and 20 dioscins, were identified from the roots, stems, corms, leaves, and flowers of Juandan Baihe (Lilium lancifolium). Among them, 30 compounds were reported for the first time from this plant. The root and corm extracts demonstrated significant hypoglycemic activities by reducing blood glucose levels from 23.76 ± 1.21 and 24.29 ± 1.35 mmol/L to 17.21 ± 1.23 and 18.78 ± 1.49 mmol/L, respectively (P < 0.05). The roots and corms extracts could also attenuate the symptoms of polydipsia (P < 0.01), polyphagia (P < 0.05), and weight loss caused by diabetes.@*Conclusion@#This study clarifies that the roots of Juandan Baihe (Lilium lancifolium) are rich in regalosides and dioscins for the first time, and have significant hypoglycemic activities, providing the foundation for the comprehensive utilization of this plant and the development of hypoglycemic drugs.

6.
Chinese Journal of Natural Medicines (English Ed.) ; (6): 658-669, 2023.
Article in English | WPRIM | ID: wpr-1010979

ABSTRACT

Pien Tze Huang (PTH) was documented as an imperial prescription composed of Notoginseng Radix, Calculus Bovis, Snake Gallbladder, and Musk. It is famous in China and Asian countries due to its excellent effects in heat clearing, detoxifying, swelling reduction, and pain relieving. Modern pharmacological studies demonstrate that PTH shows excellent effects against various inflammatory diseases, liver diseases, and cancers. This review summaries the pharmacological effects, clinical applications, and mainchemical components of PTH. More importantly, its potential quality markers (Q-markers) were then analyzed based on the "five principles" of Q-markers under the guidance of Traditional Chinese Medicine theory, including transfer and traceability, specificity, efficacy, compatibility, and measurability. As a result, ginsenosides Rb1, ginsenoside Rg1, ginsenoside Rd, ginsenoside Re, notoginsenoside R1, dencichine, bilirubin, biliverdin, taurocholic acid, and muscone are considered as the Q-markers of PTH. These findings will provide guidance and assistance for the construction of a quality control system for PTH.


Subject(s)
Humans , Ginsenosides/pharmacology , Drugs, Chinese Herbal/pharmacology , Medicine, Chinese Traditional , Neoplasms , Quality Control , China
7.
China Pharmacy ; (12): 1358-1362, 2023.
Article in Chinese | WPRIM | ID: wpr-974685

ABSTRACT

OBJECTIVE To explore the extraction process of volatile oil from the stems, leaves and roots of Glehnia littoralis, analyze the chemical components of the volatile oil from the stems, leaves and roots of G. littoralis, and preliminarily evaluate its in vitro antifungal activity. METHODS Based on the steam distillation method, single factor test and orthogonal experiment were conducted to optimize the extraction method of volatile oil from the stems, leaves and roots of G. littoralis. The chemical components of the volatile oil from the stems, leaves and roots of G. littoralis were identified by using gas chromatography-mass spectrometry (GC-MS) technology and their relative contents were calculated. The antifungal activity of volatile oils from the stems, leaves and roots of G. littoralis against Fusarium solani, Fusarium incarnatum, Fusarium oxysporum, Aspergillus parasiticus and Aspergillus flavus was determined by paper diffusion method. RESULTS The optimal extraction process of G. littoralis was solid-liquid ratio of 1∶15, distillation time of 5 hours, and KCl concentration of 15%. Eleven components were identified from the volatile oil of the stems and leaves of G. littoralis, and a total of eight components were identified from the volatile oil of the roots. Ginsenethinol was a common component in the volatile oil from the stems, leaves and roots of G. littoralis, its contents in the stems and leaves, roots were 38.21% and 74.02%, respectively. The volatile oil from the stems, leaves and roots of G. littoralis had a certain E-mail:zwhjzs@126.com inhibitory effect on F. solani, F. incarnatum, F. oxysporum, A. parasiticus and A. flavus, especially volatile oil from the stems and leaves. CONCLUSIONS There is a significant difference in chemical components of the volatile oil between the roots, stems and leaves of G. littoralis, both of which have certain in vitro antifungal activity.

8.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 209-221, 2023.
Article in Chinese | WPRIM | ID: wpr-973151

ABSTRACT

Trib. Lorantheae used as traditional Chinese materia medica has a long history. There are 41 genera of Trib. Lorantheae, of which 6 belong to China, all have medicinal value, mainly distributed in Southwest, Southern, and Central and Southern China, with abundant resources. Twenty-two species of Trib. Lorantheae are used as medicinal materials or herbs in China. It mainly includes Taxillus. chinensis, T. sutchuenensis, Scurrula parasitica, Loranthus tanakae, Dendrophthoe pentandra, S. ferruginea, etc., of which T. chinensis is the most widely used. The main chemical components of Trib. Lorantheae include flavonoids, terpenoids, sterols, phenylpropanoids, curcumins, phenolic acids, violate oils, sugars, and other compounds. Modern studies show that the extracts and monomer compounds of Trib. Lorantheae have various pharmacological effects such as anti-inflammation, anti-tumor, anti-oxidation, anti-osteoporosis, bacteriostasis, anti-virus, and lowering blood sugar, blood pressure, and lipid. It is believe that most active components related to their pharmacological effects are flavonoids, most of which are the main pharmacodynamic substances of the parasitic plants of Trib. Lorantheae, playing an important role in anti-inflammation, anti-tumor, anti-oxidation, anti-osteoporosis, and other pharmacological effect. This paper systematically summarized the literature and data on plants of Trib. Lorantheae and reviewed their chemical components and pharmacological effects, which provided references for the research, development, and utilization of Trib. Lorantheae.

9.
Chinese journal of integrative medicine ; (12): 89-95, 2023.
Article in English | WPRIM | ID: wpr-971322

ABSTRACT

The primary chemical components of Astragalus membranaceus include polysaccharides, saponins, flavonoids, and amino acids. Recent studies have shown that Astragalus membranaceus has multiple functions, including improving immune function and exerting antioxidative, anti-radiation, anti-tumor, antibacterial, antiviral, and hormone-like effects. Astragalus membranaceus and its extracts are widely used in clinical practice because they have obvious therapeutic effects against various autoimmune diseases and relatively less adverse reaction. Multiple sclerosis (MS) is an autoimmune disease of central nervous system (CNS), which mainly caused by immune disorder that leads to inflammatory demyelination, inflammatory cell infiltration, and axonal degeneration in the CNS. In this review, the authors analyzed the clinical manifestations of MS and experimental autoimmune encephalomyelitis (EAE) and focused on the efficacy of Astragalus membranaceus and its chemical components in the treatment of MS/EAE.


Subject(s)
Animals , Humans , Astragalus propinquus/chemistry , Multiple Sclerosis/drug therapy , Encephalomyelitis, Autoimmune, Experimental/metabolism , Drugs, Chinese Herbal/chemistry , Polysaccharides
10.
China Pharmacy ; (12): 303-308, 2023.
Article in Chinese | WPRIM | ID: wpr-961663

ABSTRACT

OBJECTIVE To provide reference for exploring alternative resources of Gentiana rigescens from the plants of Gentiana. METHODS The contents of four components (gentiopicroside, swertiamarin, swertioside and amarogentin) in the roots and rhizomes from 3 plants of Gentiana (G. rigescens, G. cephalantha, G. delavayi) were determined by high-performance liquid chromatography (HPLC). The chemical compositions in the above roots and rhizomes were identified by ultra-performance liquid chromatography electrospray ionization quarter-time of flight mass spectrometry (UPLC-ESI-Q-TOF-MS), and the differences were analyzed by principal component analysis (PCA). RESULTS Four active components such as gentiopicroside, swertiamarin, swertioside and amarogentin were detected in the roots and rhizomes of G. rigescens and G. cephalantha, and the contents of the four components were similar in both. The contents of gentiopicroside in the root and rhizome of G. cephalantha and G.rigescens were more than four times of the limit standard of the Chinese Pharmacopoeia (Part Ⅰ) in 2020; However, only swertiamarin, swertioside and amarogentin were detected in the roots and rhizomes of G.delavayi, and the contents of swertioside and amarogentin were 34.12 and 8.81 times of those of G. rigescens, respectively. In addition, a total of 33 compounds 术。E-mail:515227235@qq.com were identified from the roots and rhizomes of 3 plants of Gentiana by UPLC-ESI-Q-TOF-MS, mainly iridoids. Additionally, G. rigescens and G. cephalantha contained xantones, G. delavayi contained flavonoids. PCA showed that there was a small difference between G. rigescens and G. cephalantha; however, there was a big difference between G. delavayi and G. rigescens. CONCLUSIONS The difference between the roots and rhizomes of G. cephalantha and G. rigescens from the same origin is small and there is substitutability; while the difference in the chemical components from roots and rhizomes between G. delavayi and G. rigescens is great and G. delavayi cannot be used as medicine instead of G. rigescens.

11.
China Journal of Chinese Materia Medica ; (24): 4115-4123, 2023.
Article in Chinese | WPRIM | ID: wpr-1008607

ABSTRACT

Eight heterocyclic compounds and twelve phenolic glycosides were separated from the water extract of Dendrobium officinale flowers through chromatographic techniques, such as Diaion HP-20 macroporous adsorption resin column chromatography(CC), silica gel CC, ODS CC, Sephadex LH-20 CC, and preparative high performance liquid chromatography(PHPLC). According to the spectroscopic analyses(MS, ~1H-NMR, and ~(13)C-NMR) and optical rotation data, the compounds were identified as dendrofurfural A(1), 2'-deoxyadenosine(2), 4-[2-formyl-5-(hydroxymethyl)-1H-pyrrol-1-yl] butanoic acid(3), 4-[2-formyl-5-(methoxymethyl)-1H-pyrrol-1-yl] butanoic acid(4), 1-(2-hydroxyethyl)-5-(methoxymethyl)-1H-pyrrole-2-carbaldehyde(5), 5-(methoxymethyl)-1H-pyrrole-2-carbaldehyde(6), methyl 5-(hydroxymethyl)-furan-2-carboxylate(7),(S)-5-hydroxymethyl-5H-furan-2-one(8), 2-methoxyphenyl-1-O-β-D-glucopyranoside(9), arbutin(10), isotachioside(11), 2,6-dimethoxy-4-hydroxyphenol-1-O-β-D-glucopyranoside(12), orcinol glucoside(13), tachioside(14), gastrodin(15), 4-O-β-D-glucopyranosylvanillyl alcohol(16), 2,6-dimethoxy-4-hydroxymethylphenol-1-O-β-D-glucopyranoside(17), icariside D_2(18), 4-formylphenyl-β-D-glucopyranoside(19), and vanillin-4-O-β-D-glucopyranoside(20). Among them, compound 1 is a new furfural benzyl alcohol condensate, with the skeleton first found in Dendrobium. Compounds 2-9, 11, 13, and 19 are reported from Dendrobium for the first time, and compounds 14 and 18 are reported for the first time from D. officinale. Compounds 11 and 14 showed moderate DPPH radical scavenging capacity, and compounds 11-14 demonstrated potent ABTS radical scavenging capacity, possessing antioxidant activity.


Subject(s)
Dendrobium , Butyric Acid , Glycosides/analysis , Phenols/analysis , Heterocyclic Compounds , Flowers/chemistry
12.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 1-12, 2023.
Article in Chinese | WPRIM | ID: wpr-969593

ABSTRACT

ObjectiveTo characterize the chemical constituents of Dayuanyin based on ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap MS). MethodThe detection was performed on a Thermo Acclaim™ RSLC 120 C18 column(2.1 mm×100 mm, 2.2 μm), the mobile phase was acetonitrile(A)-0.1% formic acid aqueous solution(B) for gradient elution (0-7.5 min, 10%-19%A; 7.5-12 min, 19%-22.5%A; 12-23 min, 22.5%-27%A; 23-27 min, 27%-56%A; 27-35 min, 56%-84%A; 35-36 min, 84%-90%A), the flow rate was 0.3 mL·min-1, and the column temperature was 30 ℃. The data were collected in the positive and negative ion modes by heated electrospray ionization(HESI), and the detection range was m/z 80-1 200. Combining the retention time of the reference substance, fragment ions, databases such as PubChem and related literature, Xcalibur 3.0 was used to identify the chemical constituents of Dayuanyin. ResultA total of 161 compounds were identified, including 14 alkaloids, 60 flavonoids, 16 terpenoids, 26 saponins, 18 phenylpropanoids, 16 organic acids and 11 others. ConclusionThe established method can effectively and quickly identify the chemical components in Dayuanyin, and clarify its chemical composition, which can provide a basis for the development of compound preparations of this famous classical formula.

13.
China Pharmacy ; (12): 796-801, 2023.
Article in Chinese | WPRIM | ID: wpr-969574

ABSTRACT

OBJECTIVE To identify the chemical components of Changtong oral liquid (CTOL),and to provide reference for the basic research and secondary development of its pharmacological substances. METHODS UHPLC-Orbitrap HRMS technique was adopted. CTOL sample was separated on a Hypersil Gold column with mobile phase consisted of 0.1% formic acid (containing 5 mmol/L ammonium formate)-acetonitrile (gradient elution). The eluent was detected in positive and negative ion modes using an electrospray ionization source. The data was processed by Xcalibur 4.3 and Compound Discoverer 3.3 software. The primary and secondary mass spectra data of each compound were collected. The unknown compounds were identified according to the mass spectrometry library of the instrument and the network databases mzCloud,mzVault,etc. Through matching with the pharmacology database and analysis platform of the traditional Chinese medicine system,the chemical components could be attributed to the traditional Chinese medicine. RESULTS Fifty-three chemical components were identified and analyzed from CTOL,such as 24 flavonoids,8 quinones,5 phenylpropanoids,4 sugars and glycosides,5 organic acids,3 amino acids,1 alkaloid,1 phenolic and 2 other compounds. Among them,12 components were derived from Salvia miltiorrhiza,9 from Citrus aurantium,7 from Rheum palmatum,4 from Angelica sinensis,1 from Magnolia officinalis,16 from Glycyrrhiza uralensis,and 4 from many kinds of medicinal materials. CONCLUSIONS CTOL mainly contains flavonoids,quinones and phenylpropanoid compounds,and its chemical components mainly come from G. uralensis,S. miltiorrhiza and C. aurantium.

14.
China Pharmacy ; (12): 666-670, 2023.
Article in Chinese | WPRIM | ID: wpr-965502

ABSTRACT

OBJECTIVE To analyze chemical components of Shenqi tiaoshen formula (SQTS). METHODS UPLC-QE-MS method was adopted. The determination was performed on ACQUITY UPLC BEH C18 column with mobile phase consisted of 0.1% formic acid solution-0.1% formic acid acetonitrile solution (gradient elution) at the flow rate of 0.4 mL/min. The column temperature was set at 40 ℃ , and the sample size was 5 μL. The electrospray ionization source was used to scan positive and negative ions, and the scanning range was m/z 100-1 500. Combined with TCMSP, PubChem and other databases, SQTS active component database was established and the components were identified in combination with relevant literature. RESULTS & CONCLUSIONS Totally 131 chemical components were identified from SQTS, including 23 terpenoids, 22 flavonoids, 21 phenylpropanoids, 12 alkaloids, 11 phenols, 9 amino acid derivatives, 4 fatty acyls, 3 organic acids and others, such as rutin, citrinin, synephrine, cinnamic acid and ginsenoside Rg1,etc. The cracking process of the main components involved the breaking of glycosidic bonds, dehydration, etc.

15.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 275-282, 2023.
Article in Chinese | WPRIM | ID: wpr-964969

ABSTRACT

Malignant tumor poses a threat to human health and life. The incidence and fatality rate of malignant tumor have been on the rise. The currently available therapies are radiotherapy and chemotherapy, which cause severe adverse reactions and irreversible damage and thus influence the quality of life of patients. Therefore, it is urgent to find new, safe and effective antitumor drugs. Chinese medicinals are safe with little adverse reactions and long-lasting effect in the treatment of tumor, which have attracted the attention of scholars. Amid the advancement of medical research, more and more anti-tumor components have been extracted from Chinese medicinals. Phellinus is a valuable Chinese medicinal material, and the chemical components mainly include polysaccharides, flavonoids, triterpenes, and polyphenols, which have anti-inflammatory, hypoglycemic, liver-protecting, and anti-tumor effect. The chemical components of Phellinus can inhibit various malignant tumors such as lung cancer, gastric cancer, colon cancer, and melanoma. It exerts the anti-tumor effect by inhibiting proliferation and metastasis of tumor cells, inducing apoptosis and autophagy of the cells, suppressing tumor angiogenesis, and regulating the immunity. In addition, it can enhance efficacy, reduce toxicity, and boost the sensitivity in the radiotherapy and chemotherapy. In this paper, articles were retrieved from China National Knowledge Infrastructure (CNKI), Web of Science, Pubmed, and Google Scholar with keywords such as "Phellinus, chemical components, and anti-tumor", and then the chemical components of Phellinus and the anti-tumor mechanisms were summarized. The findings are expected to lays a basis for further development and clinical application of this medicinal.

16.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 53-60, 2022.
Article in Chinese | WPRIM | ID: wpr-940796

ABSTRACT

ObjectiveTo explore the meridian tropism of components in Bupleuri Radix (Chaihu, CH) based on the model of nonalcoholic steatohepatitis (NASH) and clarify the substance basis of the meridian tropism of CH in Xiaoyaosan (XYS) by means of principal component analysis. MethodEighty SPF male C57BL/6 mice were randomly assigned into 8 groups, with 10 mice in each group. Except that the blank group was fed with the methionine choline-sufficient (MCS) diet, the other mice were fed with methionine choline-deficient (MCD) diet for 4 weeks to establish the nonalcoholic steatohepatitis (NASH) model. After the established model was confirmed by hematoxylin-eosin (HE) staining, the mice were administrated with corresponding drugs by gavage once a day for 4 weeks. Specifically, the 8 groups were XYS group (2.874 g·kg-1), XYS-CH group (2.445 g·kg-1), XYS-CH+volatile oils (Vol, 0.163 mg·kg-1) group, XYS-CH+polysaccharides (Pol, 24.067 mg·kg-1) group, XYS-CH+flavones (Fla, 2.241 mg·kg-1) group, and XYS-CH+saponins (Sap, 2.746 mg·kg-1) group. The model group and the blank group were administrated with the same volume of normal saline. After the last administration, the mice were sacrificed for the collection of blood and liver tissue. The pathological changes of liver were observed by HE staining and oil red O staining. Enzyme linked immunosorbent assay (ELISA) kits were used to determine the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) in serum as well as malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in liver. SPSS Statistics 23 was used for principal component analysis and comprehensive evaluation to determine the substance basis of the meridian tropism of CH in NASH mice. ResultCompared with the blank control group, the modeling led to hepatocyte swelling, increased fat vacuoles, and appearance of inflammatory cells. Further, the modeling elevated the levels of ALT, AST, TG, TC, and LDL and lowered the HDL level in serum, and it increased the MDA level and decreased the SOD, CAT, and GSH-Px levels in liver. Compared with the model group, the administration of XYS and XYS-CH in combination with the components of CH alleviated the oxidative damage in liver (P<0.05). The comprehensive score of the pharmacological efficacy was in a descending order as follows: XYS > XYS-CH+Sap > XYS-CH+Fla > XYS-CH+Pol > XYS-CH+Vol > XYS-CH. Among the chemical components of CH, Sap had the best effect. ConclusionSap lowers the blood lipid level, regulates the abnormal lipid metabolism, and alleviates the oxidative damage of liver, which is the substance basis for CH to exert the meridian tropism in liver.

17.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 217-226, 2022.
Article in Chinese | WPRIM | ID: wpr-940639

ABSTRACT

Smallanthus sonchifolius, a plant resource with both medicinal and edible values, has been taken as fruit for a long history. Studies have proved that phenolic acids, flavonoids, sesquiterpene lactones, and fructooligosaccharides are the major compounds in S. sonchifolius. The extract of S. sonchifolius demonstrates noticeable antioxidant, anti-inflammatory, antimicrobial, and anti-cancer effects, as well as the activities of lowering blood glucose level, regulating intestinal function and so on. The rhizomes and leaves of S. sonchifolius contain abundant phenolic acids, mainly caffeic acid and its derivatives, which endow S. sonchifolius with remarkable antioxidant effect. Moreover, these substances can reduce blood glucose by improving insulin sensitivity. Fructooligosaccharides are abundant in the tuber of this plant, which can improve intestinal function by regulating intestinal flora. The sesquiterpene lactones in glandular trichomes on the leaf surface can inhibit the proliferation of cancer cells, among which uvedafolin and enhydrofolin have particularly strong activities. Furthermore, the sesquiterpene lactones have obvious inhibitory effect on Gram-positive bacteria. In terms of structure, the number of epoxy groups is linked to the strength of anticancer and antimicrobial effects. In addition, S. sonchifolius contains other compounds such as volatile oils, fatty acids, sterols, diterpenes, p-hydroxyacetophenone derivatives, and octulosonic acid derivatives, thereby exhibiting the pharmacological effects of treating Alzheimer's disease, protecting kidney, and lowering blood lipids. However, the isolation and identification of the main compounds in S. sonchifolius need further exploration, and the mechanism of action remains to be studied. Here we summarized the principal chemical components and pharmacological activities of S. sonchifolius, aiming to give a clue for the comprehensive development and utilization of this plant.

18.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 250-258, 2022.
Article in Chinese | WPRIM | ID: wpr-940576

ABSTRACT

Malignant tumors are currently seriously endangering human health and life, which has become one of the main causes of death in China. In modern Western medicine, they are mainly tackled by surgery, chemotherapy, and radiotherapy, but the death toll continues to rise year by year. At present, most of the anti-tumor chemotherapeutics used in clinical practice have toxic and side effects, affecting the anti-tumor efficacy and the conditions after treatment. Long-term medication will also induce drug resistance, making the good anti-tumor effect difficult to be achieved. With the vigorous development of traditional Chinese medicine (TCM), it has played a crucial role in the fight against tumors. It is believed in TCM that "heat toxin" is one of the important causes of tumors. Therefore, the methods of clearing away heat and removing toxin are often emphasized in the treatment of tumors, and the resulting outcomes are satisfactory. There are many Chinese herbs and Chinese herbal compounds classified into the heat-clearing and toxin-removing type. Xihuangwan, a classic heat-clearing prescription, is composed of Calculus Bovis, Moschus, Olibanum, and Myrrh and has the effects of clearing away heat, removing toxin, eliminating edema, and dissipating mass, which is mainly used to treat carbuncle, pustule, scrofula, multiple abscess, and cancer caused by heat-toxin obstruction. In modern clinical practice, it has been employed in patients with lung cancer, breast cancer, gastric cancer, liver cancer, colorectal cancer, and other malignant tumors, especially during the advanced stage, as a routine or adjuvant treatment for alleviating their clinical symptoms and improving their quality of life. The main active components of Xihuangwan are pentacyclic triterpenoids (such as masticinic acids), volatile oils, steroids (like porcine deoxycholic acid), and bilirubin, which have been proved effective in anti-tumor. This paper reviewed the prescription source, pharmaceutical research, clinical anti-tumor research, and pharmacological mechanisms of Xihuangwan, which has provided reference for further expanding the anti-tumor applications of Xihuangwan and enhancing its secondary development.

19.
Journal of Pharmaceutical Practice ; (6): 339-346, 2022.
Article in Chinese | WPRIM | ID: wpr-935033

ABSTRACT

Objective To rapidly explore the chemical components of Xiaotan Tongfu formula, and to provide scientific basis for the basic research and clinical treatment of the formula. Methods Analysis was performed on an Agilent 1290 ultra-performance liquid chromatography system coupled with an Agilent 6530 accurate quality Q-TOF/MS system, by using a Waters ACQUITY UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm), with a gradient elution applying 0.1% aqueous formic acid solution and acetonitrile as a mobile phase. The flow rate was 0.3 ml/min. The column temperature was 30°C. The injection volume was 1 μl, and the detection wavelength was 254 nm. Mass spectrometry (MS) data were collected in both positive and negative ESI ion modes. Components in the formula were identified by using the in-house compound database, and comparing the retention time (tR), MS1 and MS2 data with the standard compounds, and the online compound MS database. Results A total of 55 compounds were identified from Coptis coptidis, Pseudomonas solani, Rhubarb, Araceae artemisiae and Pinellia chinensis. Conclusion The established UHPLC-Q-TOF/MS method could systematically and accurately identify the chemical components from Xiaotan Tongfu formula, and provided a reference for the quality marker selection and the research on the active ingredient.

20.
China Journal of Chinese Materia Medica ; (24): 2480-2490, 2022.
Article in Chinese | WPRIM | ID: wpr-928136

ABSTRACT

The fingerprint of Boenninghausenia albiflora var. albiflora was established by ultra performance liquid chromatography(UPLC), and the content of 12 active components including chlorogenic acid was determined. Multivariate statistical analysis was used to explore the indicator components of B. albiflora var. albiflora and a comprehensive evaluation system was created for the quality of B. albiflora var. albiflora. In this study, 33 batches of B. albiflora var. albiflora with different sources were collected and studied, and the UPLC fingerprint of B. albiflora var. albiflora was developed. There were 37 common peaks, of which 12 components were identified, and the content of these 12 components was measured. In combination of the common peaks and the content of chemical components, multivariate statistical analysis was performed, and the results showed that 6 components [daphnoretin, isoimperatorin, astragalin, imperatorin, neochlorogenic acid, and isoquercitrin(weight coefficient>0.1)] were selected as chemical markers for the quality of B. albiflora var. albiflora. Technique for order of preference by similarity to ideal solution(TOPSIS) analysis and chemometrics revealed that the quality of S32, S28 and S29 were superior, while that of S12, S7 and S16 were inferior. The quality evaluation method of B. albiflora var. albiflora constructed in this study was accurate and reliable, with simpleness and easiness to operate. It is suggested that the 6 above-mentioned active components could be used as indicator components for quality control of B. albiflora var. al-biflora. The samples were harvested during the flowering and fruiting period, which is from the beginning of July to the end of August.


Subject(s)
Chromatography, High Pressure Liquid/methods , Chromatography, Liquid , Drugs, Chinese Herbal/chemistry , Multivariate Analysis , Quality Control
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