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1.
Article in Chinese | WPRIM | ID: wpr-1004146

ABSTRACT

【Objective】 To learn the production efficient of platelet components among prefecture-level blood stations in China, to provide supporting data for those blood stations to optimize the production mode of platelet components and continuously improve production efficiency and supply capacity. 【Methods】 The data from 2017 to 2020 was obtained from 24 prefecture-level blood stations who were the members of the practice comparison network for blood institutes in China. The collection units of apheresis platelets, the number of dual-collections of apheresis platelets and plasma, the average apheresis units of one platelet apheresis procedure, the discarded rate of apheresis platelets, the amount of expired apheresis platelets and the amount of apheresis platelets issued were collected. For concentrated platelets, the prepared amount of platelet concentrates and the amount of expired platelet concentrates were collected; both the quantity of qualified and issued concentrated platelets were submitted for statistical analysis.The total output and efficiency of platelet components were calculated based on the collected data. 【Results】 The average annual growth rate of apheresis platelets collection in 24 prefecture-level blood stations was 12.23%, accounting for 99.80% of the total platelet output; the average collection unit of one platelets apheresis procedure was 1.75; from 2019 to 2020, only 5 blood stations performed dual-collection of platelet and plasma during one apheresis procedure; the discarded rate of apheresis platelets was 0.28%, of which 0.007% was due to expiration. A total of 1 621.2 therapeutic units of concentrated platelets were prepared, and 13.03% of them was discarded due to the expiration. The production efficiency of platelet components was 97.56%, of which the production efficiency of apheresis platelets was 97.61% and the production efficiency of concentrated platelets was 74.43%. 【Conclusion】 There are large regional differences in the supply capacity of platelet components in prefecture-level blood stations. Apheresis platelets are the main resource of platelet components product, and the collection capacity is increasing over the years with the characteristics of high production efficiency and low expiration scrapping rate. However, the preparation of concentrated platelets are still limited with relatively low production and high expiration discarded rate.

2.
Article in Chinese | WPRIM | ID: wpr-607463

ABSTRACT

Objective To establish a nucleic acid testing assay for bacterial detection with meeting automatic requirement,and use it preliminarily to detect bacterial contamination in concentrated platelet.Methods Optimizing the bacterial DNA extraction methods,evaluating the sensitivity and specificity of the optimized assay with different bacterial strains,and applying the assay on bacterial detection for 100 units of concentrated platelet.Results The optimized extraction methods met the criteria of automatic testing.The sensitivity of the assay is 10,150,65,15 CFU/mL for Escheriehia coli,Stphylococcus aureus,Bacillus subtilis,and Pseudomonas aeruginas respectively.There was no non-specific amplification detected.And the assay could detect 1 contaminated bacterial from 100 units of concentrated platelet as same as BactALT bacterial culture.Conclusions Nucleic acid testing for bacterial genome 16S DNA could detect common contaminated bacterial sensitively and fast from platelet product.This assay could have the same effect when compared to bacterial culture methods when it was applied in concentrated platelet products.

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