How did coronavirus disease 2019 affect autonomic balance in young individuals? Analysis by heart rate variability

SUMMARY OBJECTIVE: The aim of this study was to demonstrate the effect of coronavirus disease 2019 on the cardiovascular autonomic system using heart rate variability in young individuals. METHODS: The study was designed retrospectively by scanning the 24-h Holter electrocardiography records of patients who applied to the Ankara Abdurrahman Yurtaslan Oncology Education and Research Hospital Cardiology outpatient clinic. The study group consisted of 492 patients under the age of 40 years, who did not have additional comorbidities or medication use and had prolonged symptoms after coronavirus disease 2019 during the pandemic. The control group, including 401 patients, was determined during the pre-pandemic period (before December 2019). Heart rate variability parameters were evaluated by scanning the 24-h Holter electrocardiography records of the patients and compared with the non-coronavirus disease 2019 group. RESULTS: The median age of participants was 30 years. Standard deviation of normal RR intervals (SDNN) ≤100 ms was more prevalent in the study group (27 (6.7%) vs 73 (14.8%), p<0.001). In univariate logistic regression analysis, the presence of coronavirus disease 2019 [(OR 2.41, 95%CI 1.52-3.83), p<0.001] and age [(OR 1.04, 95%CI 1.01-1.07), p=0.016] had a significant effect on the probability of SDNN≤100. In multivariate logistic regression analysis, the presence of coronavirus disease 2019 [(OR 2.42, 95%CI 1.52-3.85), p<0.001] and age [(OR 2.42, 95%CI 1.52-3.85), p=0.016] had a significant effect on the probability of SDNN≤100. Frequency domain measures such as, high-frequency values were significantly higher in the study group (p=0.029). The study group's low-frequency/high frequency ratio was significantly lower (p=0.019). The low-frequency/high-frequency ratio's cut-off value was ≤2.77. for determining the differentiation between coronavirus disease 2019 positive and negative cases in the receiver operating characteristic analysis. The sensitivity rate was 80.7%. The area under the curve value is 0.546 (p=0.019). CONCLUSION: This study showed that coronavirus disease 2019 causes reduced heart rate variability and increased parasympathetic activity in young patients. This may explain the prolonged symptoms after coronavirus disease 2019 infection.

Determining Sex from Thai Radius: Discriminant Function Analysis Including the Dry Bone Weight as an Effective Variant / Determinación del Sexo a Partir del Radio Tailandés: Análisis de Función Discriminante que Incluye el Peso del Hueso Seco como Variante Eficaz

SUMMARY: In forensic anthropology, the radius bone has been shown to determine the sex of human remains in a number of different populations. The dry mass and growth of long bones, including the radius, are associated with sex hormone levels; however, the use of bone weight to determine sex has not been sufficiently investigated. The aim of this study was to apply bone morphometric parameters, including maximum length of radius (MLR), circumference at the midshaft of radius (CMR), and weight of radius (WR), to 400 sample radii from a Northeastern Thai population. Univariate and multivariate discriminant functions of all parameters were systemically applied. Equations for calculating sex classification were also determined. Descriptive data analysis showed significant sexual dimorphism in all variables (p < 0.05). The canonical correlation was highest in CMR (0.772) and the ratio of weight to length (0.747). Multivariate discriminant function analysis showed that the measured indices of the right radius were slightly greater than those of the left radius. The parameters demonstrating the highest values of the standardized canonical discriminant function coefficients were CMR (Rt. = 0.496, Lt. 0.431) and WR (Rt. = 0.681, Lt. = 0.715). Moreover, the results of the multivariable (stepwise method) indicated that the best accuracy rates for using combinations of CMR and WR were 94 % (right side) and 92 % (left side). In conclusion, the weight of the radius (rather than the length) is an effective parameter in determining sex.

En antropología forense, se ha demostrado que el hueso radio determina el sexo de los restos humanos en varias poblaciones diferentes. La masa seca y el crecimiento de los huesos largos, incluido el radio, están asociados con los niveles de hormonas sexuales; sin embargo, el uso del peso de los huesos para determinar el sexo no se ha investigado suficientemente. El objetivo de este estudio fue aplicar parámetros morfométricos óseos, incluida la longitud máxima del radio (LMR), la circunferencia en la mitad del radio (CMR) y el peso del radio (PR), a 400 radios de muestra de una población del noreste de Tailandia. Se aplicaron sistémicamente funciones discriminantes univariadas y multivariadas de todos los parámetros. También se determinaron ecuaciones para calcular la clasificación por sexo. El análisis descriptivo de los datos mostró un dimorfismo sexual significativo en todas las variables (p < 0,05). La correlación canónica fue mayor en CMR (0,772) y la relación peso-longitud (0,747). El análisis de función discriminante multivariante mostró que los índices del radio derecho eran ligeramente mayores que los del radio izquierdo. Los parámetros que demostraron los valores más altos de los coeficientes de la función discriminante canónica estandarizada fueron CMR (Rt. = 0,496, Lt. 0,431) y PR (Rt. = 0,681, Lt. = 0,715). Además, los resultados del método multivariable (método paso a paso) indicaron que las mejores tasas de precisión al usar combinaciones de CMR y PR fueron del 94 % (lado derecho) y del 92 % (lado izquierdo). En conclusión, el peso del radio (más que la longitud) es un parámetro eficaz para determinar el sexo.

Sex Determination from the Patella in a Thai Population / Determinación del Sexo a Partir de la Rótula en una Población Tailandesa

SUMMARY: The aim of this study was to derive the equations for sex determination and to test the accuracy of discrimination between sexes using measurements of the patella in the Thai population. The sample comprised 254 Thai skeletons (134 males and 120 females) aged between 22 and 97 years, with an average age of 63.3years, obtained from the Osteology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai. Six variables were measured in 254 pairs of normal patellae. The mean values of patellar measurements in males were significantly greater than those in females for all variables (P<0.05). Statistical analysis further showed that all variables were useful in sex determination. Stepwise discriminant function analysis yielded an accuracy of 83.2 % (83.8 % male, 82.5 % female) and 85.5 % (85.3 % male, 85.8 % female) for the left and right patellae, respectively. These results suggested that measurement of the right patella is preferable for skeletal sexing. If this is not available, the forensic specialist can use the left patella instead, with an average accuracy of more than 80.0 % in giving a correct classification. The findings of this study demonstrate that the patella is an important bone in sex determination. They suggest that either the left or right patella can be used for sex determination, especially in the Northern Thai population.

El objetivo de este estudio fue derivar las ecuaciones para la determinación del sexo y probar la precisión de la discriminación entre sexos utilizando mediciones de la patela en la población tailandesa. La muestra estuvo compuesta por 254 esqueletos tailandeses (134 hombres y 120 mujeres) con edades comprendidas entre 22 y 97 años, con una edad promedio de 63,3 años, del Centro de Investigación y Capacitación en Osteología de la Facultad de Medicina de la Universidad de Chiang Mai, Chiang Mai. Se midieron seis variables en 254 pares de patelas normales. Los valores medios de las mediciones patelares en los hombres fueron significativamente mayores que los de las mujeres, para todas las variables (P <0,05). Además, el análisis estadístico mostró que todas las variables fueron útiles para la determinación del sexo. El análisis de la función discriminante por pasos arrojó una precisión del 83,2 % (83,8 % hombres, 82,5 % mujeres) y 85,5 % (85,3 % hombres, 85,8 % mujeres) para las patelas izquierda y derecha, respectivamente. Estos resultados sugieren que la medición de la patela derecha es preferible para la determinación del sexo en esqueletos. En caso de no estar disponible y para dar una clasificación correcta, el especialista forense puede utilizar la patela izquierda, con una precisión promedio de más del 80,0 %. Los hallazgos de este estudio demuestran que la patela es un hueso importante en la determinación del sexo. Se sugiere que se pueda utilizar la patela izquierda o derecha para determinar el sexo, especialmente en la población del norte de Tailandia.

Machine Learning Algorithms for Sex Classification by Using Variables of Orbital Structures: A Computed Tomography Study / Algoritmos de Aprendizaje Automático para la Clasificación del Sexo Mediante el Uso de Variables de Estructuras Orbitales: Un Estudio de Tomografía Computarizada

SUMMARY: Since machine learning algorithms give more reliable results, they have been used in the field of health in recent years. The orbital variables give very successful results in classifying sex correctly. This research has focused on sex determination using certain variables obtained from the orbital images of the computerized tomography (CT) by using machine learning algorithms (ML). In this study 12 variables determined on 600 orbital images of 300 individuals (150 men and 150 women) were tested with different ML. Decision tree (DT), K-Nearest Neighbour (KNN), Logistic Regression (LR), Random Forest (RF), Linear Discriminant Analysis (LDA), and Naive Bayes (NB) algorithms of ML were used for unsupervised learning. Statistical analyses of the variables were conducted with Minitab® 21.2 (64-bit) program. ACC rate of NB, DT, KNN, and LR algorithms was found as % 83 while the ACC rate of LDA and RFC algorithms was determined as % 85. According to Shap analysis, the variable with the highest degree of effect was found as BOW. The study has determined the sex with high accuracy at the ratios of 0.83 and 0.85 through using the variables of the orbital CT images, and the related morphometric data of the population under question was acquired, emphasizing the racial variation.

Dado que los algoritmos de aprendizaje automático dan resultados más fiables, en los últimos años han sido utilizados en el campo de la salud. Las variables orbitales dan resultados muy exitosos a la hora de clasificar correctamente el sexo. Esta investigación se ha centrado en la determinación del sexo utilizando determinadas variables obtenidas a partir de las imágenes orbitales de la tomografía computarizada (TC) mediante el uso de algoritmos de aprendizaje automático (AA). En este estudio se probaron 12 variables determinadas en 600 imágenes orbitales de 300 individuos (150 hombres y 150 mujeres) con diferentes AA. Se utilizaron algoritmos de AA de árbol de decisión (DT), K-Nearest Neighbour, regresión logística (RL), Random Forest (RF), análisis discriminante lineal (ADL) y Naive Bayes (NB) para el aprendizaje no supervisado. Los análisis estadísticos de las variables se realizaron con el programa Minitab® 21.2 (64 bits). La tasa de ACC de los algoritmos NB, DT, KNN y RL se encontró en % 83, mientras que la tasa de ACC de los algoritmos ADL y RFC se determinó en % 85. Según el análisis de Sharp, la variable con el mayor grado de efecto se encontró como BOW. El estudio determinó el sexo con alta precisión en las proporciones de 0,83 y 0,85 mediante el uso de las variables de las imágenes de TC orbitales, y se adquirieron los datos morfométricos relacionados de la población en cuestión, enfatizando la variación racial.

Factors influencing gender preference and awareness against sex determination in antenatal women in rural tertiary hospital in South India

Background: Discrimination begins in the womb even before a baby is born. Sex ratio is an important social indicator that measures extent of prevailing equity between males and females in society and a balanced sex ratio is essential for a stable society. The main objective of this study is to find out the awareness of sex determination and to study the factors influencing gender preference among antenatal women.Methods: A cross-sectional, hospital-based study was done among antenatal women at Adichunchanagiri institute of medical sciences, B.G. Nagara, Mandya. Information regarding the type of family, number of family members, socioeconomic status, gender preference, factors influencing gender preference and obstetric details was collected after taking consent from the antenatal women attending the outpatient department in Adichunchanagiri institute of medical sciences as well as inpatient antenatal women. Details of about the awareness of sex determination and the perception of women regarding sex determination were also collected.Results: Total 114 pregnant women were included in this study. Among multigravidas 31.4% of them had male preference based on the sex of 1st baby being female, 44.7% (51) participants had son preference and 36% (41) had daughter preference and 19.3% (22) participants had no gender preference. Among study participants 95 (83.3%) of them were aware of punishment regarding sex determination and 19 (16.7%) of them were unaware of legal punishment.Conclusions: Awareness regarding sex determination facilities was found to be increased and pregnant women were also aware of the punishable act regarding sex determination. Factors influencing gender preference mainly included the gender of the first baby in multigravidas and the socioeconomic status.

La superación del profesor de Educación Física para desarrollar la autodeterminación, en educandos con discapacidad intelectual

La superación continua de los profesores de Educación Física constituye una necesidad que ha transcendido diferentes generaciones de docentes, determinada por el desarrollo creciente de la ciencia y la técnica, las exigencias del tercer perfeccionamiento educacional cubano, las demandas en la preparación y capacitación del profesorado y la necesidad de su incidencia en la formación de un egresado apto y capaz de desarrollar no solo habilidades y capacidades físicas, sino también para la vida, que les permitan actuar en el medio social, familiar y laboral. El objetivo de esta investigación consistió en diseñar una estrategia pedagógica de superación para transformar los modos de actuación de los profesores de Educación Física, en la dirección del proceso de enseñanza-aprendizaje, en función del desarrollo de habilidades de autodeterminación en los educandos en situación de discapacidad intelectual. Los métodos investigativos utilizados en el orden teórico y empírico fueron el análisis y síntesis, el histórico-lógico, el inductivo-deductivo, la modelación y el sistémico-estructural-funcional; además de la entrevista, la observación, la encuesta, el análisis de documentos, el criterio de expertos y la triangulación múltiple, los que permitieron revelar las causales que inciden en el normal desarrollo de las habilidades de autodeterminación, así como elaborar la estrategia. Con su implementación se perfeccionó el desempeño profesional y personal de los profesores y los educandos, quienes egresaron con el empoderamiento de habilidades para la toma de decisiones, la realización de elecciones, la resolución de problemas y el establecimiento de metas y objetivos.

O aperfeiçoamento contínuo dos professores de Educação Física constitui uma necessidade que transcendeu as diferentes gerações de professores, determinada pelo crescente desenvolvimento da ciência e da tecnologia, pelas exigências do terceiro melhoramento educacional cubano, pelas exigências na preparação e formação de professores e pela necessidade de seu impacto na formação de um egresso qualificado, capaz de desenvolver não apenas competências e habilidades físicas, mas também para a vida, que lhe permitam atuar no ambiente social, familiar e de trabalho. Discapacidade intelectual. Os métodos investigativos utilizados na ordem teórica e empírica foram análise e síntese, histórico-lógico, indutivo-dedutivo, modelagem e sistêmico-estrutural-funcional; Além da entrevista, observação, inquérito, análise documental, peritagem e triangulação múltipla, que permitiram revelar as causas que afetam o normal desenvolvimento das competências de autodeterminação, bem como desenvolver a estratégia. Com a sua implementação melhorou-se o desempenho profissional e pessoal de professores e alunos, que se formaram com a capacitação de competências para tomar decisões, fazer escolhas, resolver problemas e estabelecer metas e objetivos.

The continuous improvement of Physical Education teachers constitutes a need that has transcended different generations of teachers, determined by the growing development of science and technology, the demands of the third Cuban educational improvement, the demands in the preparation and training of teachers and the need for its impact on the training of a qualified graduate capable of developing not only physical skills and abilities, but also for life, which allow them to act in the social, family and work environment. The objective of this research was to design a pedagogical improvement strategy to transform the modes of action of Physical Education teachers, in the direction of the teaching-learning process, based on the development of self-determination skills in students in situations of intellectual disability. The investigative methods used in the theoretical and empirical order were analysis and synthesis, historical-logical, inductive-deductive, modeling and systemic-structural-functional; In addition to the interview, observation, survey, document analysis, expert judgment and multiple triangulations, which allowed us to reveal the causes that affect the normal development of self-determination skills, as well as to develop the strategy. With its implementation, the professional and personal performance of teachers and students was improved, who graduated with the empowerment of skills for making decisions, making choices, solving problems and establishing goals and objectives.

Knowledge and attitude of antenatal mothers on the preconception and prenatal diagnostic techniques act attending Government Maternity Hospital in Andhra Pradesh: A cross-sectional study

Background: Girl children are vulnerable to gender biases throughout the economic and social strata. From the moment of conception, a girl’s existence is an ongoing struggle for survival, development, and growth. Gender equality has gained recognition in the past 10 years as essential to a country’s social and economic progress and overall health. Aims and Objectives: The aim of the study was to know the awareness and attitude of antenatal mothers regarding the preconception and prenatal diagnostic techniques (PCPNDT) act, gender preference, and their awareness regarding related social issues. Materials and Methods: A hospital-based cross-sectional study was conducted after obtaining ethics committee approval and consent from 200 antenatal mothers attending a maternity hospital from September to December 2022. Data were collected using a predesigned, pre-tested, semi-structured questionnaire. The analysis was done with Statistical Package for the Social Sciences software version 26.0. Results: Out of 200 study subjects, 80% were in the age group of 19–25 years, 60.5% were aware of the PCPNDT act, and 43.8% of mothers had knowledge about the PCPNDT act from hospital sources. Of the 56% of mothers who preferred a male child, 35.7% said it was first being a girl child. In this study, awareness of the PCPNDT act was significantly associated with age, area of living, religion, education, occupation, and socio-economic status, but not with type of family. Conclusion: Despite better knowledge regarding the PCPNDT act, the attitude toward sex determination, female foeticide, and son preference has to be changed. For this reason, strict implementation of the PCPNCDT act is essential.

Biological Identification of Skulls in Indonesian and Thai Populations: Ancestry Estimation, Sex Determination, Stature Estimation, and Age Estimation / Identificación Biológica de Cráneos en Poblaciones de Indonesia y Tailandia: Estimación de Ascendencia, Determinación del Sexo, Estimación de Estatura y Estimación de Edad

SUMMARY: This review article will present an overview of biological profiles in forensic utilities. The biological profile of the skull in the existing literature can help to identify humans, especially if the condition of the victim found is a result of mutilation or a bomb explosion. When it comes to the precision of identifying skeletal remains, the human skull is frequently cited as being first in the estimation of age and ancestry and second in terms of sex and stature. It can be an alternative to assessing the following biological parameters: sex, age, stature, and ancestry. The implementation of biological profiles in the identification process is very important considering that some cases require the assistance of forensic anthropology. This review article shows the importance of the value of skulls. The method that can be applied is craniometry which can be used to determine sex, age, stature, and estimated ancestry. Different results will occur depending on the completeness of the skull. Therefore, estimation formulas have different accurate results. Discriminant function analysis has been performed on various measurement sets and its discriminant power has been validated by many researchers. Geometric morphometric analysis has become the main tool for shape analysis and many attempts have been made to use it in analyzing skulls. Several methods supported by technology have also been developed. It is hoped that the review article will show significant differences in results between studies in Thailand and Indonesia, even though they are in the same racial group.

Este artículo presenta una descripción general de los perfiles biológicos en las utilidades forenses. El perfil biológico del cráneo en la literatura existente puede ayudar a identificar a los humanos, especialmente si la condición en la que se encuentra la víctima es el resultado de una mutilación o la explosión de una bomba. Cuando se trata de la precisión en la identificación de restos óseos, el cráneo humano se cita con frecuencia como el primero en la estimación de edad y ascendencia y el segundo en términos de sexo y estatura. Puede ser una alternativa para evaluar los siguientes parámetros biológicos: sexo, edad, estatura y ascendencia. La implementación de perfiles biológicos en el proceso de identificación es importante considerando que algunos casos requieren la asistencia de la antropología forense. Este artículo de revisión muestra la importancia del valor de las cnezas óseas. El método que se puede aplicar es la craneometría para determinar el sexo, la edad, la estatura y la ascendencia estimada. Se pueden obtener diferentes resultados dependiendo de la integridad del cráneo. Por lo tanto, las fórmulas de estimación tienen resultados precisos diferentes. Se ha realizado un análisis de función discriminante en varios conjuntos de medidas y muchos investigadores han validado su poder discriminante. El análisis a través de la morfometría geométrica se ha convertido en la principal herramienta para el análisis de formas y se ha utilizado frecuentemente en el análisis de cráneos. También se han desarrollado varios métodos apoyados en la tecnología. Se espera que este trabajo muestre diferencias significativas en los resultados entre los estudios realizados en Tailandia e Indonesia, aunque pertenezcan al mismo grupo racial.

Sex Determination From Foramen Magnum Measurements - An Autopsy Based Study In North Kerala Population

Establishing identity from fragmented skeletal remains is difficult when compared to other techniques available. In cases like natural disasters, bomb explosions etc. where only few fragmented remains of the body are present, then forensic anthropology can be used to establish the identity. Even though determination of sex from skull is possible, if skull fragments are only available then the importance of foramen magnum for determination of sex comes into consideration if occipital bone is present. The current study was done to find the possibility of sex determination in North Kerala population using the measurements of foramen magnum. The sagittal and transverse diameters of foramen magnum were measured using digital Vernier callipers and the values were analysed and was found that sagittal diameter of foramen magnum can be used to differentiate the sex in the present study and that the sex of an individual cannot be determined based on transverse diameter of Foramen Magnum in the given population.

Radiomorphometric Analysis of Frontal Sinus for Gender Determination in Rayalaseema

Background: In the process of identifying unidentified human remains, sex determination is an important stage. In order to identify remains and determine gender, frontal sinus radiography has been performed. Aim: The present study was aimed to determine the frontal sinus measurement and assess its application in gender determination among the rayalaseema population. Materials and Methods: A total of 40 individuals (20-male and 20-female) aged between 25-30 years were included in the study for which height, width and area of frontal sinus was determined using posteroanterior view radiographs and were transferred to the Radiant Dicom viewer for the measurements of frontal sinuses. Statistical analysis used: The data thus obtained was analysed by Student’s t-test for comparison of the means of the dimensions measured for the two genders. The stepwise method was used to select the variables and composition of the mathematical model, where the variables were added and removed until the model was defined. Results: Significant differences were observed for frontal sinus height, width and area between males and females. Conclusion: Determining the sex of unidentified human remains is a crucial step in the identification process. Frontal sinus radiography has been carried out to locate the remains and establish the gender

In the same storm, but on different boats: The social determination of mental health during COVID-19 / Na mesma tempestade, mas em barcos diferentes: a determinação social da saúde mental na COVID-19

Objective The social and health scenario of the pandemic caused by COVID-19 had an impact on the mental health of the population, characterized by strong health inequities. Faced with this problem, this study aimed to analyze the predictor variables of mental health in Brazilians during the pandemic, identifying the most vulnerable groups. Method An online survey was carried out, with a non-probabilistic sample of 1.397 Brazilians, who answered a biodemographic and general health questionnaire, analyzed using descriptive and analytical statistics. Results It was identified that being female, non-heterosexual, unemployed, with low income, and history of mental health comorbidities are predictors of mental health problems. In addition to these, the sample comparisons revealed other groups with greater susceptibility: single and divorced, without religion, with history of COVID-19, maintaining social distancing, and bereaved. Conclusion There are groups with greater vulnerability to mental health problems, requiring health policies for prevention and health promotion that are appropriate for different social groups.

Objetivo O cenário social e sanitário da pandemia de COVID-19 repercutiram na saúde mental da população, marcada por fortes iniquidades em saúde. Diante desse problema, o presente estudo objetivou analisar as variáveis preditoras da saúde mental de brasileiros durante a pandemia, identificando os grupos mais vulneráveis. Método Realizou-se um levantamento online, com amostra não probabilística de 1.397 brasileiros, que responderam a um questionário biodemográfico e ao Questionário de Saúde Geral, analisados por meio de estatística descritiva e analítica. Resultados Identificou-se que ser do sexo feminino, não heterossexual, desempregado, com baixa renda e comorbidade prévia em transtornos mentais são preditores de problemas em saúde mental. Além desses, as comparações amostrais revelaram outros grupos com maior susceptibilidade: solteiros e divorciados, sem religião, com histórico de COVID-19, em distanciamento social e enlutados. Conclusão Existem grupos com maior vulnerabilidade a problemas de saúde mental, sendo necessárias políticas de saúde de prevenção e promoção da saúde adequadas aos diferentes grupos sociais.

Assessement of bone age agreement between the sauvegrain and greulich and pyle methods / Avaliação da concordância da idade óssea entre os métodos de sauvegrain e greulich e pyle

ABSTRACT Objective: To evaluate the intra and inter observer agreement of the Sauvegrain, Greulich and Pyle methods. Material and methods: This is an observational, retrospective and cross-sectional study ethically approved by opinion 6,192,391. 100 radiographic images of the elbow and 100 of the left wrist and hand were collected from children whose images were selected by a researcher who did not carry out the evaluations. The Sauvegrain, Greulich and Pyle methods were used to determine bone age. We provided a detailed explanation of each method and the evaluators received a file with the study images. After three weeks, the exams were randomized and the radiograms were reevaluated. Of the 100 patients in group A, 61 (61%) were boys and 39 (39%) were girls. In group B, 67 (67%) were boys and 33 (33%) were girls. Four statistical analyzes were used: correlation; intraclass correlation; analysis using the Bland-Altman graph; differences between groups. Results: Intra and interobserver agreement between groups was considered excellent. Conclusions: Despite the excellent agreement, group A presented a significantly better value than B. Biological ages show a greater difference compared to chronological ages in group A. In group B, skeletal and chronological ages do not show statistical difference according to the accuracy test. Level of Evidence III, Cross-Sectional Observational Study.

RESUMO Objetivo: Avaliar a concordância intra e interobservadores dos métodos de Sauvegrain e Greulich e Pyle. Material e métodos: Trata-se de um estudo observacional, retrospectivo e transversal, aprovado eticamente pelo parecer 6.192.391. Foram coletadas cem imagens radiográficas do cotovelo e cem do punho e mão esquerdos de crianças, selecionadas por um pesquisador que não realizou as avaliações. Utilizou-se os métodos de Sauvegrain e Greulich e Pyle para determinar a idade óssea. Uma explicação detalhada de cada método foi realizada, e os avaliadores receberam um arquivo com as imagens do estudo. Após três semanas, os exames foram randomizados e os radiogramas reavaliados. Dos cem pacientes do grupo A, 61(61%) eram meninos e 39(39%) meninas. No grupo B, 67(67%) eram meninos e 33(33%) meninas. Quatro análises estatísticas foram utilizadas: correlação; correlação intraclasse; análise pelo gráfico de Bland-Altman; e diferenças entre grupos. Resultados: A concordância intra e interobservador entre os grupos foi considerada excelente. Conclusões: Apesar da concordância excelente, o grupo A apresentou valor significantemente melhor que o B. As idades biológicas apresentam maior diferença frente as idades cronológicas no grupo A. No grupo B, as idades esqueléticas e cronológicas não apresentam diferença estatística segundo o teste de acurácia. Level of Evidence III, Cross-Sectional Observational Study .

Promoção de territórios saudáveis: assessoria técnica à habitação social no Setor 1 da Colônia Juliano Moreira / Promotion of healthy territories: Technical advice on social housing in Sector 1 of Colônia Juliano Moreira

RESUMO O artigo propõe contribuir para a reflexão sobre princípios e estratégias promotoras de saúde em territórios vulnerabilizados, tendo como objetivo específico avaliar o papel que pode ser desempenhado por uma política e serviço público de base local, permanente e participativo, desenvolvido por meio de assessoria técnica com enfoque na requalificação do espaço da moradia e do habitat que favoreça a população mais vulnerabilizada e promova soluções positivas em sua qualidade de vida. O trabalho tem sua fundamentação teórico-metodológica no campo da saúde urbana, referenciada na perspectiva crítica da complexidade do fenômeno saúde-doença, mais especificamente quando relaciona a vulnerabilidade socioambiental e os condicionantes do ambiente construído à saúde coletiva. O artigo utiliza como caso referência a experiência desenvolvida pela Fiocruz Mata Atlântica, da Fundação Oswaldo Cruz, e busca verificar a identificação dos fatores determinantes e de processos de determinação social da saúde, bem como a apropriação de princípios e estratégias de ação desse campo disciplinar. Os resultados parciais dessa experiência em curso apresentam avanços não somente nas transformações objetivas dos fatores determinantes relacionados com o ambiente construído, mas também na organização social e na articulação intersetorial nesse contexto, assim como na construção de referências para políticas públicas.

ABSTRACT The article proposes to contribute to reflection on health promoting principles and strategies in vulnerable territories, aiming at the specific objective to evaluate the role that can be played by a local, permanent, and participatory policy and public service, developed through technical assistance with a focus on improving the housing space and the habitat that favors the most vulnerable population and promotes positive solutions in their quality of life. The work has its theoretical methodological foundation in the field of urban health, referenced from the critical perspective of the complexity of the health-disease phenomenon, more specifically when it relates the social and environmental vulnerability and the conditions of the built environment to collective health. The article uses as a reference the experience developed by FIOCRUZ Mata Atlântica, of the Oswaldo Cruz Foundation, and seeks to verify the identification of social determinants and processes of social determination of health as well as the appropriation of principles and strategies of action of this disciplinary field. The partial results of this ongoing experience have advances not only in the objective transformations of determining factors related to the built environment, but also in the social organization and intersectoral articulation in this context, as well as the construction of references for public policies.

A necessidade de construção de assistência e Vigilância em Saúde no contexto das mudanças climáticas - 'um passo à frente e você não estará mais no mesmo lugar' / The need to build health care and surveillance in the context of climate change - 'one step forward and you will no longer be in the same place'

RESUMO Artigo crítico-reflexivo, à luz da Determinação Social do processo saúde/doença, que objetivou articular as concepções e as abordagens da vigilância em saúde à problematização das questões complexas de saúde que exigem a produção de cuidado articulado à territorialidade, à intersetorialidade e à interseccionalidade, além de concatenado à prática assistencial na Atenção Primária à Saúde, em contexto global de intensas e incertas mudanças climáticas. Para alcançar o objetivo, realizou-se um ensaio teórico no qual se desenvolveram argumentos teórico-analíticos caracterizados por articular pesquisas empíricas em relação ao tema da necessidade de construção de assistência e vigilância em saúde territorialmente articuladas e coletivamente construídas no contexto das mudanças climáticas. Para tanto, este manuscrito se organiza em três itens: 1) problematizar a 'Determinação Social da Saúde e o enfrentamento dos agravos de relevância epidemiológica'; 2) debater as 'mudanças climáticas e os desafios persistentes e o enfrentamento dos agravos infectocontagiosas no Brasil'; 3) apresentar 'Estratégias participativas e construção de abordagens democráticas na assistência e na vigilância em saúde'. A Vigilância Popular em Saúde tem sido caracterizada como prática democrática que articula ações de vigilância concatenadas às realidades territoriais, definindo-se como experiência que tem apontado novos horizontes para uma prática territorialmente articulada e coletivamente construída.

ABSTRACT This critical-reflexive article, in light of the Social Determination of the health/disease process, aims to articulate the conceptions and approaches of health surveillance to problematize complex health issues that require the production of care articulated to territoriality, intersectorality and intersectionality, in addition to being linked to care practice in Primary Health Care, in a global context of intense and uncertain climate change. The methodological path outlined to achieve the objective was to carry out a theoretical essay, in which theoretical-analytical arguments were developed, characterized by articulating empirical research in relation to the theme of the need to build territorially articulated and collectively constructed health care and surveillance in the context of climate change. To this end, this manuscript is organized into three items: 1) problematize the 'Social Determination of Health and coping with diseases of epidemiological relevance'; 2) discuss 'climate change and persistent challenges and combating infectious diseases in Brazil'; 3) present 'Participatory strategies and construction of democratic approaches in health care and surveillance'. Popular Health Surveillance has been characterized as a democratic practice that articulates surveillance actions linked to territorial realities, characterizing itself as an experience that has pointed out new horizons for a territorially articulated and collectively constructed practice.

Analysis of chemical constituents and comprehensive quality evaluation of Rhamni Songoricae Fructus / 中国药房

OBJECTIVE To identify the chemical constituents of Rhamni Songoricae Fructus and to establish their fingerprints and the method for simultaneous determination of four constituents to comprehensively evaluate the quality of Rhamni Songoricae Fructus. METHODS The chemical constituents in Rhamni Songoricae Fructus were qualitatively analyzed by ultra-performance liquid chromatography quadrupole-time-of-flight tandem mass spectrometry. The fingerprints of 15 batches of Rhamni Songoricae Fructus were established by HPLC and chemometric analysis was performed by using SPSS 26.0 and SIMCA 14.1 software； the contents of quercetin， kaempferol， kaempferide and emodin were determined by the same method. RESULTS A total of 35 constituents were identified， including 28 kinds of flavonoids， 5 kinds of anthraquinones and 2 kinds of organic acids. A total of 19 common peaks were identified in the HPLC fingerprints， recognizing quercetin， kaempferol， kaempferide and emodin. The similarities between HPLC fingerprints of 15 batches of samples and control chromatograms were greater than 0.9. The results of cluster analysis showed that 15 batches of samples were divided into 2 classes， of which S1-S5， S7 and S9 were one class and the rest were one class， similar to the results of principal component analysis. The results of the orthogonal partial least squares- discriminant analysis showed that the variable importance projections of peaks No. 2， 6， 1， 11 （quercetin）， 3， 14， 8， 10， 19 （emodin）， 5 were all greater than 1. The contents of quercetin， kaempferol， kaempferide and emodin ranged from 0.710 to 10.478 mg/g， 0.236 to 0.660 mg/g， 0.334 to 3.039 mg/g， and 0.261 to 0.504 mg/g. CONCLUSIONS The constructed chemical constituent identification， fingerprint and content determination methods are simple， feasible and reproducible， which combined with chemometric analysis can be used for comprehensive evaluation of the quality of Rhamni Songoricae Fructus.

Comparison of Differential Saponin Contents and Pro-angiogenic Activities on Zebrafish Between Freeze-dried and Sun-dried Panacis Quinquefolii Radix Decoction Pieces / 中国实验方剂学杂志

ObjectiveTo analyze and determine the differential components of freeze-dried and sun-dried Panacis Quinquefolii Radix（PQR）， and to compare the differences in their pro-angiogenic activities. MethodFingerprints of freeze-dried and sun-dried PQR were established based on ultra performance liquid chromatography（UPLC）， and chemometrics methods such as principal component analysis（PCA） and orthogonal partial least squares-discriminant analysis（OPLS-DA） were combined to determine the differential saponin composition of the two decoction pieces， and six representative saponins were selected and their contents in freeze-dried and sun-dried PQR were determined by UPLC. Transgenic zebrafish line Tg（fli1a∶EGFP） embryos fertilized for 24 h were selected， and different doses of 70% methanol extracts of freeze-dried and sun-dried PQR（10， 30 mg·L-1） were used to intervene in normal zebrafish and in a zebrafish model of intersegmental vascular（ISV） injury induced by vascular endothelial growth factor（VEGF） receptor tyrosine kinase inhibitor Ⅱ（PTK787）， then the development of subintestinal vein（SIV） and ISV of zebrafish was observed， SIV diameter， mean number of crossings and mean number of germinations were determined， and the ISV vascular index was calculated， in order to compare the pro-angiogenic activities of the two decoction pieces. ResultThe similarity of the fingerprints of freeze-dried and sun-dried PQR decoction pieces was>0.950， and 17 common peaks were identified， of which 6 common peaks were designated as peak 6（ginsenoside Rg1）， peak 7（ginsenoside Re）， peak 8（ginsenoside Rb1）， peak 11（ginsenoside Rc）， peak 13（ginsenoside Rb2）， and peak 16（ginsenoside Rd）， respectively. A total of 11 differential saponin components were screened by PCA and OPLS-DA， indicating that there were some differences in the contents of the components in the two decoction pieces. The results of determination showed that the contents of ginsenosides Rg1， Re， Rb1 and Rb2 in freeze-dried PQR were higher than those in sun-dried PQR， while the contents of ginsenosides Rc and Rd were lower than those in sun-dried PQR（P<0.05， P<0.01）. In the study of the pro-angiogenic effect on normal zebrafish embryos， compared with the blank group， and the SIV vessel diameter， mean germination rate and mean crossover rate were significantly higher in the high-dose groups of freeze-dried and sun-dried PQR（P<0.01）， and the vessel diameter， mean numbers of crossings and germinations in the freeze-dried PQR group were higher than those of the sun-dried PQR group（P<0.05）. In the study of the pro-angiogenic effect on zebrafish embryos with ISV injury， the development of ISV in the model group was significantly inhibited when compared with the blank group， compared with the model group， different dose groups of freeze-dried and sun-dried PQR could promote the growth and sprouting of ISV， and the number of normal blood vessels in the freeze-dried PQR group was significantly higher than that in the sun-dried PQR group at the same dosage（P<0.05）. ConclusionFreeze-drying can effectively avoid the loss and secondary transformation of ginsenosides in PQR， and its angiogenic activity is better than that of sun-dried PQR， which can provide a reference for the production and development of high-quality PQR decoction pieces.

Simultaneous determination of three constituents in Chunyang Zhengqi capsules by HPLC / 药学实践与服务

Objective To establish method for simultaneous determination of hesperidin, cinnamaldehyde and eugenol in Chunyang Zhengqi capsules by high performance liquid chromatography. Methods The column was Agilent PorosheⅡ 120 EC-C18 (4.6 mm×150 mm, 4 μm). The mobile phase was acetonitrile-water with gradient elution. The column temperature was 35℃. The flow rate was 1.0 ml/min, and the detection wavelength was 284 nm. Results The methodological verification showed that hesperidin, cinnamaldehyde and eugenol had a good linearity (r≥0.999 9). The precisions were less than 2.0%. The average recovery was between 98.0% and 101.9%. The stability and repeatability of RSD were also less than 3.0%, which met the requirements of method validation. Conclusion The method is simple, stable, reproducible and accurate, which could be used to the quality control of Chunyang Zhengqi capsules.

Determination of the Contents of Three Lignans in Dendrobium fimbriatum Hook / 广州中医药大学学报

Objective To establish the method for content determination of three lignans of Dendrobium Fimbriatum Hook..Methods The lignans in Dendrobium tasselii were identified by high-performance liquid chromatography/multi-stage mass spectrometry(HPLC-ESI/MSn)coupled with ultraviolet absorption spectrometry(UV)coupled with retention time localization of high-performance liquid chromatography(HPLC).The separation was carried out on a Kromasil 100-5 C18 column(4.6 mm×250 mm,5 μm)using a gradient elution of acetonitrile-0.1%formic acid solution as the mobile phase,the volume flow rate was 0.8 mL·min-1 and the column temperature was 35℃,and the mass spectrometry was performed using an ESI ion source with the data collected in the negative ion mode.The HPLC content was determined on the same column as that of MS analysis,with the mobile phase methanol + acetonitrile(V/V=1∶1)-0.01 mol/L ammonium acetate solution,gradient elution,flow rate of 0.8 mL·min-1,column temperature of 40℃,and detection wavelength of 215 nm.Results Syringaresinol di-O-glucoside and(-)-Syringaresinol 4-O-β-D-glucopyranoside and DL-Syringaresinol were identified from Dendrobium fimbriatum Hook.,and the results of content determination showed that the linear ranges of above three components were respectively 0.1701-3.4020,0.1020-2.0400,0.0403-0.8060 μg(r≥0.9995),the average recoveries were in the range of 97.71%-101.67%,and the relative standard deviations(RSDs)were all less than 3.0%.The contents of Syringaresinol di-O-glucoside and(-)-Syringaresinol 4-O-β-D-glucopyranoside and DL-Syringaresinol in the 10 batches of samples were 0.7779-1.3852,0.0734-0.1966,0.0295-0.1882 mg·g-1.Conclusion This research method can provide a reference basis for the quality evaluation method of Dendrobium fimbriatum Hook..

GC Fingerprint and Multi-component Content Determination of Xiangsha Yangwei / 医药导报

Objective To establish the method of fingerprint and content determination of multi-component for Xiangsha Yangwei pill by gas chromatography(GC).Methods The GC fingerprint of Xiangsha Yangwei pill was found,and the peak attribution was carried out.The contents of limonene,eucalyptol,camphor,borneol,bornyl acetate,patchouli alcohol,pogostone,and α-cyperone were determined.Results The fingerprint similarity of 56 batches of Xiangsha Yangwei pill were 0.33-0.99,28 common peaks were confirmed,and 14 known components were identified.Limonene,eucalyptol,camphor,borneol,bornyl acetate,patchouli alcohol,pogostone and α-cyperone showed good linearity within the determined ranges(14.30-286.08,24.52-490.44,16.14-322.88,9.40-187.95,15.39-307.83,25.78-515.60,19.95-398.90,and 24.87-497.30 μg·mL-1).The average recoveries were 101.20％,97.90％,93.97％,94.23％,102.94％,100.54％,99.16％,and 98.31％;with the RSDs were 2.41％,1.48％,1.65％,2.00％,1.93％,2.30％,2.07％,and 2.38％,respectively.The concentrations of eight components were 0.2-959.1,0.3-420.4,1.0-542.6,0.0-64.5,0.0-364.2,0.0-339.6,0.0-130.7,0.0-82.0 μg·g-1,respectively.Conclusion The fingerprint and multi-component determination method can be used for the quality control and evaluation of Xiangsha Yangwei pill.

Content Determination of Alkaloids and Catalpol in Different Proportions of Qianjin Huanglian Pills / 医药导报

Objective To establish an HPLC method for the determination of alkaloids(epiberberine,coptisine,palma-tine,berberine)and catalpol in different ratios(1∶1,1∶10)of ancient and modern Qianjin Huanglian Pills,and to compare the differences in their contents.The content differences were compared to preliminarily evaluate the differences in the efficacy of Qianjin Huanglian Pills in the treatment of diabetes under different preparation processes and different ratios.Methods The alkaloid solvent was methanol∶ hydrochloric acid(100∶1).The detection conditions were as follows:C18 column,acetonitrile-0.05 mol·L-1 potassium dihydrogen phosphate solution(50∶50),detection wavelength 345 nm,column temperature 30℃,flow rate 1 mL·min-1,injection volume 10 μL.The catalpol solution was methanol∶ water(20∶80).The detection conditions were as follows:chromatographic column C18 column,methanol-0.1%phosphoric acid solution(1∶ 99),detection wavelength 210 nm,column temperature 30℃,flow rate 1 mL·min-1,injection volume 10 μL.Results The established method was spe-cific,and the separation effect of the five components was good.It exhibited a good linear relationship(R2＞0.999)in their respec-tive linear ranges.The repeatability,precision,stability,and sample recovery rate all met the requirements.The content of four alka-loids in the ancient method 1∶1 was the highest,and the content of catalpol was the lowest.The content of four alkaloids in the ancient method 1∶10 was the lowest;the content of 1∶1 in the present method was higher than that in the ancient method 1∶10,and the content of berberine in the present method 1∶10 was slightly lower than that in the present method 1∶1,and the rest were higher than that in the present method 1∶1.The PCA results showed that the chemical composition contents of the four kinds of Qianjin Huanglian pills were very different.Conclusion The method is simple,accurate,and reproducible,making it suitable for the quality control of Qianjin Huanglian Pills.It provides a theoretical basis for exploring the difference in efficacy of Qianjin Huanglian Pills.