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1.
Rev. Soc. Bras. Med. Trop ; 55: e0373, 2022. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1376351

ABSTRACT

ABSTRACT Background: Aedes aegypti is currently controlled with synthetic larvicides; however, mosquitoes have become highly resistant to these larvicides and difficult to eradicate. Studies have shown that insecticides derived from fungal extracts have various mechanisms of action that reduce the risk of resistance in these mosquitoes. One possible mechanism is uncontrolled production of reactive oxygen species (ROS) in the larvae, which can cause changes at the cellular level. Thus, the crude extract of Xylaria sp. was evaluated to investigate the oxidative effect of this extract in A. aegypti larvae by quantifying the oxidative damage to proteins and lipids. Methods: The larvicidal potential of the crude extract of Xylaria sp. Was evaluated, and the extract was subsequently tested in human lung fibroblasts for cytotoxicity and ROS production. ROS level was quantified in the larvae that were killed following exposure to the extract in the larvicide test. Results: The crude extract of Xylaria sp. Caused cytotoxicity and induced ROS production in human lung fibroblasts and A. aegypti larvae, respectively. In the larvicide trial, the extract showed an LC50 of 264.456 ppm and an LC90 of 364.307 ppm, and was thus considered active. The extract showed greater oxidative damage to lipids and proteins, with LC90 values of 24.7 µmol MDA/L and 14.6278 ×10-3 nmol carbonyl/ mg protein, respectively. Conclusions: Crude extracts of Xylaria sp. induced oxidative stress that may have caused the mortality of A. aegypti larvae.

2.
Article in Chinese | WPRIM | ID: wpr-924050

ABSTRACT

Objective To study the effects of endophytic fungus Epichloë bromicola SH09 on the plant growth and accumulation of active components in Salvia miltiorrhiza, and improve the quality of medicinal plant S. miltiorrhiza. Methods E. bromicola SH09 solid bacterial fertilizer was prepared and co-cultured with S. miltiorrhiza for 60 d and 120 d. Four morphological indexes, fresh weight of roots, dry weight of roots, and the contents of four tanshinones and two phenolic acids in the roots of S. miltiorrhiza from treated group and control group were assayed, respectively. Results After 60 d and 120 d co-culture, E. Bromicola SH09 significantly increased the tiller number, plant height, leaf number, leaf area, fresh weight of roots, dry weight of roots, and the content of tanshinones and phenolic acids in S. miltiorrhiz. Conclusion The endophytic fungus E. bromicola SH09 can effectively promote the plant growth and improve the accumulation of active components in S. miltiorrhiza, which not only broadens the new ecological functions of endophytic fungi, but also improves the quality of medicinal plant S. miltiorrhiza.

3.
Article in Chinese | WPRIM | ID: wpr-879133

ABSTRACT

Nine secondary metabolites(S)-5-hydroxy-4-methylchroman-2-one(1), 4-methoxynaphthalene-1,5-diol(2), 8-methoxynaphthalene-1,7-diol(3), 1,8-dimethoxynaphthalene(4),(2R,4S)-2,3-dihydro-2-methyl-benzopyran-4,5-diol(5),(2R,4R)-3,4-dihydro-4-methoxy-2-methyl-2H-1-benzopyran-5-ol(6), 7-O-α-D-ribosyl-2,3-dihydro-5-hydroxy-2-methyl-chromen-4-one(7),(R)-3-methoxyl-1-(2,6-dihydroxyphenyl)-butan-1-one(8) and helicascolide A(9) were isolated from endophytic fungus Cladosporium sp. JJM22 by using column chromatographies of silica gel and ODS, and semi-preparative HPLC. Their structures were analyzed on the basis of spectroscopic and chemical data, especially NMR and MS. All isolated compounds were evaluated for their anti-inflammatory activities by examining the inhibitory activities on nitric oxide(NO) production induced by lipopolysaccharide in mouse macrophage RAW264.7 cells in vitro. Compounds 2-4 showed inhibitory activities.


Subject(s)
Animals , Benzopyrans , Cladosporium , Fungi , Mice , Molecular Structure , Rhizophoraceae
4.
Rev. argent. microbiol ; 52(1): 43-49, mar. 2020. graf
Article in English | LILACS-Express | LILACS | ID: biblio-1155684

ABSTRACT

Abstract Dark septate endophytes (DSE) are a heterogeneous group of fungi, mostly belonging to the Phylum Ascomycota, that are involved in a mutualistic symbiosis with plant roots. The aim of this study is to evaluate the behavior of two strains of DSE isolated from wheat roots of two cropping areas in the province of Buenos Aires, Argentina, against some agrochemicals. Of all the isolates obtained, two strains were identified as Alternaria alternata and Cochliobolus sp. These DSE were found to be tolerant to glyphosate, carbendazim and cypermethrin when evaluated at the recommended agronomic dose (AD), 2 AD and, in some cases, 10 AD. This work contributes to the study of the biology of this group of fungi and their tolerance in the presence of xenobiotics widely used in agriculture.© 2019 Asociaci´on Argentina de Microbiolog´ıa. Published by Elsevier Espa˜na, S.L.U. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/bync-nd/4.0/).


Resumen Los endófitos septados oscuros (DSE) son un grupo heterogéneo de hongos que participan de una simbiosis mutualista con raíces de plantas, perteneciendo principalmente al Phylum Ascomycota. El objetivo de este estudio fue aislar DSE de raíces de trigo proveniente de dos áreas de cultivo de la provincia de Buenos Aires y evaluar el comportamiento de dos cepas de DSE aisladas de raíces de trigo frente a algunos agroquímicos en dos áreas de cultivo de la provincia de Buenos Aires. De todos los aislamientos obtenidos se seleccionaron dos cepas que se identificaron como Alternaria alternata y Cochliobolus sp. Se encontró que estos DSE son tolerantes al glifosato, el carbendazim y la cipermetrina, evaluados a las dosis agronómicas recomendadas (AD), a 2x AD y, en algunos casos, a 10x AD. Este trabajo contribuye al conocimiento de la biología de este grupo de hongos y su tolerancia a xenobióticos ampliamente utilizados en la agricultura.

5.
Article in Chinese | WPRIM | ID: wpr-846037

ABSTRACT

Objective: To study the secondary metabolites of Penicillium oxalicum, an endophytic fungus isolated from the medicinal plant Pseudostellariaheterophylla. Methods: The secondary metabolites were isolated by silica gel, Sephadex LH-20 column chromatographies, and prep-TLC methods. Their structures were elucidated by using various spectroscopic techniques including HRESIMS and NMR spectra. Results: A benzofuran compound 5,7-dihydroxy-2-methylbenzofuran-3-carboxylic acid (1), five isocoumarins including (3R,4R)-(-)-4-hydroxymellein (2), O-methylmellein (3), acremonone G (4), decarboxycitrinone (5) and decarboxyhydroxycitrinone (6), four bisabolane-type sesquiterpenoids including (7S,11S)-(+)-12-acetoxysydonic acid (7), (S)-(+)-11-dehydrosydonic acid (8), sydonic acid (9) and 1-hydroxy-boivinianin A (10), as well as two meroterpenoid-type alkaloids decaturin D (11) and decaturinC (12) were isolated. Conclusion: Compound 1 is a new benzofuran compound and named as oxafuranone A, while compounds 2-6 and 10 are characterized from P. oxalicum for the first time.

6.
Article | IMSEAR | ID: sea-210487

ABSTRACT

Ceratobasidium ramicola IBRLCM127, an endophytic fungus isolated from the rhizome of the local medicinal plantCurcuma mangga Valeton & Zijp, was found to possess significant anti-candidal activity. This fungal endophyte wascultivated in submerged fermentation system using yeast sucrose medium supplemented with host plant water extractand cultivated at 25°C, agitated at 120 rpm for 12 days. The ethyl acetate was used as a solvent to extract compoundsin the fermentative broth. The fungal ethyl acetate extract demonstrated significant inhibitory zones toward cells ofCandida albicans with minimum inhibitory concentration (MIC) value of 2.5 mg/ml, of which exerting yeastocidaleffect. The time–kill study conducted at three distinct ethyl acetate concentrations (half MIC, MIC, and 2 MICvalues) revealed that the growth of C. albicans cells was concentration-dependent. Yeastostatic activity was shownat lower concentration and yeastocidal activity was shown at higher concentration. The structural degeneration of theC. albicans cells after treated with ethyl acetate extract was observed under the scanning and transmission electronmicroscopes and the results exhibited various cell deformities including severe damage of the cell extracellularly andintracellularly which led to cell death beyond repair, thus suggesting that the extract could be a potential antifungalagent.

7.
Article | IMSEAR | ID: sea-210572

ABSTRACT

Recently, endophytic fungi have attracted attention due to their ability to produce a variety of bioactive metabolites. Inthe course of our study on endophytic fungi associated with Albertisia papuana Becc, it has been found the endophyticfungus Xylaria sp. DAP KRI-5 produce cytochalasin D. Isolation of cytochalasin D was conducted by using anopen column chromatography method. The chemical structure of cytochalasin D was deduced from 1 dimension, 2dimension nuclear magnetic resonance, and ultra-performance liquid chromatography quadrupole time of flight massspectrometry analyses and also compared with the published data. The bioproduction capacity of cytochalasin D bythe fungus Xylaria sp. DAP KRI-5 was 0.05763 g/l.

8.
Article | IMSEAR | ID: sea-210551

ABSTRACT

Recently, endophytic fungi have attracted attention due to their ability to produce a variety of bioactive metabolites. Inthe course of our study on endophytic fungi associated with Albertisia papuana Becc, it has been found the endophyticfungus Xylaria sp. DAP KRI-5 produce cytochalasin D. Isolation of cytochalasin D was conducted by using anopen column chromatography method. The chemical structure of cytochalasin D was deduced from 1 dimension, 2dimension nuclear magnetic resonance, and ultra-performance liquid chromatography quadrupole time of flight massspectrometry analyses and also compared with the published data. The bioproduction capacity of cytochalasin D bythe fungus Xylaria sp. DAP KRI-5 was 0.05763 g/l.

9.
Article | IMSEAR | ID: sea-209791

ABSTRACT

The study was carried out to investigate the antibacterial activity of an endophytic fungal isolate, Lasiodiplodiapseudotheobromae IBRL OS-64 residing in leaves of a medicinal herb, Ocimum sanctum Linn. Qualitative screeningof the antimicrobial activity was done using an agar plug assay, and the results showed that the fungal isolate wasable to inhibit all the 13 test bacteria. Three Gram-positive bacteria (methicillin-resistant Staphylococcus aureus[MRSA] ATCC 33591, Staphylococcus aureus, and Streptococcus mutans) were the most susceptible species withthe inhibition zones of ≥21 mm. The other three (Bacillus cereus ATCC 10876, Bacillus subtilis IBRL A3, andStreptococcus agalactiae) showed the inhibition zones of 11–≤20 mm of diameter. As for Gram-negative bacteria,Yersinia enterocolitica was the most susceptible to the fungal isolate with the size of inhibition zone of ≥21 mm,followed by Klebsiella pneumoniae ATCC 13883, Salmonella typhimurium, and Shigella boydii ATCC 9207 withthe inhibition zones of 11–≤20 mm, whereas Escherichia coli IBRL 0157, Proteus mirabilis, and Pseudomonasaeruginosa ATCC 27883 were the least susceptible with the inhibition zones of ≤10 mm. Quantitative screeningusing disc diffusion assay showed that the fungal ethyl acetate extract prepared from the fermentative broth(extracellular) exhibited better antibacterial activity compared to the methanolic extract prepared from the fungalbiomass (intracellular). The results showed that the ethyl acetate extract exhibited antibacterial activity against all the13 test bacteria with the inhibition zone sizes of 20.0 ± 0.3–31.3 ± 1.2 mm in diameter for Gram-positive bacteria and10.31 ± 0.6–20.1 ± 0.6 mm in diameter for Gram-negative bacteria. On the other hand, the methanolic extract onlyinhibited three Gram-positive bacteria (MRSA ATCC 33591, S. aureus, and S. mutans) with the inhibition zones of9.0 ± 0.6–11.0 ± 0.3 mm in diameter, whereas only one Gram-negative (S. typhimurium) with the inhibition zone sizeof 13.3 ± 1.5 mm diameter. The minimal inhibitory concentration (MIC) and minimum bactericidal concentration(MBC) values of the ethyl acetate extract on Gram-positive bacteria were in the range of 62.50–125.00 and 62.50–500.00 µg/mL, respectively, whereas for the Gram-negative bacteria, the MIC and MBC values were 125.00–250.00and 250.0–1000.00 µg/mL, respectively. On the other hand, the MIC and MBC values for methanolic extract againstGram-positive bacteria were 250.00–500.00 µg/mL and against Gram-negative bacteria were 1000.00 µg/mL,respectively. Both of the extracts exhibited bactericidal effects on test bacteria with the MBC/MIC ratio ≤4. Further,detail of the effects of the ethyl acetate extract on the bacterial cells was observed from the scanning electronmicroscopy photomicrographs which revealed the severity of the morphological deterioration experienced by theextract-treated cells were beyond repair, and the most possible mode of actions were by interrupting the cell wallbiosynthesis and cell membrane permeability

10.
Article in Chinese | WPRIM | ID: wpr-850691

ABSTRACT

Objective: To study the secondary metabolites of endophytic fungus Aspergillus fumigatus from Euphorbia royleana. Methods: The fermentation liquor of the fungal strain A. fumigatus was isolated and purified using various chromatographic methods. The structures of the compounds were identified by spectroscopic analysis. Results: Three compounds were isolated and their structures were identified as (R)-2-propylhexyl-2H-1,2,3-triazole-4-carboxylate (1), decumbenone A (2), and (+)-cyclopenol (3). Conclusion: Compound 1 is a new compound isolated from this fungus, which is identified as aspergillus triazolate A.

11.
Article in Chinese | WPRIM | ID: wpr-777479

ABSTRACT

In order to find new source of antifungal agents, eleven cultivable endophytic fungi were isolated from the roots,stems and leaves of Chelidonium majus by traditional method. Seven of them were identified as Colletotrichum(L1, L2, L3, S1, S3, S4, S5), and three of them were identified as Fusarium(R1,R2,R3) by morphological features and molecular biological technology. The antifungal activity test showed that all the tested fungi displayed some inhibitory activity against five common plant pathogens(C. gloeosporioides, Curvularia lunata, Pyricularia oryza, Alternaria alternate and A. brassicae), and their inhibition rate of some test items were over 60%. Among them, R1, S2, S3 and S4 were more potent than others. This study enriches the understanding of endophytes from Ch. majus and provides a basis for the study of new microbial fungicides.


Subject(s)
Alternaria , Virulence , Antibiosis , Ascomycota , Virulence , Chelidonium , Microbiology , Colletotrichum , Chemistry , Endophytes , Chemistry , Fusarium , Chemistry
12.
Natural Product Sciences ; : 228-232, 2019.
Article in English | WPRIM | ID: wpr-760568

ABSTRACT

Five secondary metabolites, including a new isopimarane derivative xylaroisopimaranin A (1), were isolated from the endophytic fungus Xylaralyce sp. (HM-1), and their structures were elucidated by 1D, 2D NMR, MS and CD spectra. Their bioactivities were performed to antibacterial, Hep G2 cells cytotoxicity and brine shrimp inhibition. The biological evaluation results showed that the xylaroisopimaranin A (1), xylabisboein B (2), griseofulvin (3) , 5-methylmellein (4) and mellein-5-carboxlic acid (5) displayed no significant Hep G2 cells cytotoxicity and antibacterial acitivity, but they inhibited the brine shrimp with IC₅₀ from 0.5 to 25 µmol/mL.


Subject(s)
Artemia , Fungi , Griseofulvin , Hep G2 Cells
13.
Article in Chinese | WPRIM | ID: wpr-802319

ABSTRACT

Objective:To study the secondary metabolites of the endophytic fungus Trichoderma harzianum,which was isolated from Physalis angulata. Method:The strains were cultured in a big-scale by oscillating incubator. Then the obtained culture liquid and mycelia were extracted by EtOAC and actone,respectively. The extracts were integrated after recovery of solvents, and then the active secondary metabolites were isolated and purified by comprehensive use of open ODS flash column,sephadex LH-20,HPLC,and LC-MS analysis techniques. Their structures were identified according to their physico-chemical properties and nuclear magnetic resonance (NMR) data. The cytotoxicities of the compounds were determined by methyl thiazolyl tetrazolium(MTT) assay. Result:Ten compounds isolated from T. harzianum were identified as destruxin A2(1),destruxin B2(2),3-isobutyl-pyrrolopiperazine-2,5-dione[cyclo (leu-pro dipeptide)](3),cyclo (Phen-pro) dipeptide(4),cyclonerodiol(5),brevianamide F(6),N-acetyltryptamine(7),9-hydroxyl-(2-methylpropyl) isobutyl phthalate(8),5-hydroxy-3-hydroxymethyl-2-methyl-7-methoxychromone(9),and phenylpropionic acid(10). Compounds 1-10 didn't exhibit significant cytotoxicity to lung cancer cell line A549 in the MTT assay (IC50 ≥ 20 mg·L-1). Conclusion:All compounds were isolated from T. harzianum for the first time.

14.
Article in Chinese | WPRIM | ID: wpr-801809

ABSTRACT

Objective: To study the chemical constituents in theendophytic fungus Nigrospora oryzae from Cordyceps. Method: It was cultured with brownrice,and isolated and purified by chromatographic procedures, and the compounds were identified by NMR,ESI-MS and other spectral methods. Result: Totally 15 compounds were identified as mellein (1),linoleic acid (2),2-(2-hydroxyethyl)phenol (3),(3R)-mellein methyl ether (4),(3R,4S)-4-hydroxymellein (5),2-hydroxybenzaldehyde (6),3-phenylpropane-1,2-diol (7),1-phenyl-1,2-ethanediol (8),cyclo-(R-Prommmm-S-Ile) (9),cyclo-(D-Pro-L-Leu) (10),2-hydroxybenzaldehyde (11),4-hydroxy-8-O-methylmellein (12),cyclo-(S-Pro-S-Phe) (13),cyclo-(D-Pro-L-Ile) (14),cyclo-(D-Pro-L-Leu) (15). Conclusion: Compounds 1-15 were isolated from this fungus for the first time.

15.
China Pharmacist ; (12): 10-14, 2018.
Article in Chinese | WPRIM | ID: wpr-705440

ABSTRACT

Objective: To study the chemical constituents of endophytic fungus Alternaria solani from Aconitum transsectum. Methods: The compounds were isolated and purified by column chromatography , and their structures were elucidated based on the spectroscopic analysis (1H-NMR, 13C-NMR and MS).Results:Totally 15 compounds were isolated from A.solani and characterized as 20-hydroxyl-ergosta-4,6,8(14),22-tetraen-3-one(1),ergone(2),stellasterin(3),episterol(4),ergosterol peroxide(5),cyclo(D)-Pro-(D)-Leu(6),cyclo-(L-Val-L-Pro)(7),cyclo-(S-Pro-S-Ile)(8),tentoxin(9),porrotoxin(10),7-dehydroxyl-zinniol(11),zinniol (12),8-zinniol methyl ether (13),3-methoxy-4-methyl-5-(3′,3′-dimethylallyloxy)-2-methoxymethyl benzoic acid (14) and pseudo-monas aeruginosa H2-sesquiterpene lactone (15).Conclusion: Totally 15 compounds are isolated from endophytic fungus Alternaria solani, and compounds 2-10 and 12-15 are isolated for the first time .

16.
China Pharmacy ; (12): 1483-1487, 2018.
Article in Chinese | WPRIM | ID: wpr-704827

ABSTRACT

OBJECTIVE:To identify lipid metabolites of endophytic fungus Aspergillus terreus from Polygonum capitatum, and to investigate their anti-multidrug resistant bacteria and anti-inflammatory effects. METHODS:GC-MS was used to analyze and identify lipid metabolites of A. terreus. MIC of lipid metabolites and main composition to 10 strains of multidrug resistant bacteria (Klebsiella pneumoniae,Proteus common,Staphylococcus epidermidis,Escherichia coli,Staphylococcus aureus,Enterobacter cloacae,Pseudomonas aeruginosa,Proteus mirabilis,Enterococcus faecium and Acinetobacter baumanii) were determined by 96-well plate microdilution method. The spread plate method was used to determine MBC of samples to bacteria. LPS-induced RAW264.7 inflammatory model was used to investigate the effects of different mass concentrations(50,100,200 μg/mL)of lipid metabolites on the release of NO and TNF-α after treated for 24 h. RESULTS:A total of 13 compounds were identified in lipid metabolites of A. terreus,among which palmitic acid,stearic acid,linoleic acid and oleic acid were main components,and relative percentages of them were 29.35%,10.87%,21.94%,34.85%. The lipid metabolites displayed anti-bacterial activity against E. coli and K. pneumonia with MICs of 12.5 mg/mL and 50 mg/mL,MBC of 25 mg/mL and 100 mg/mL,respectively. The main 4 compositions could inhibit the growth of E. coli,with MIC of 0.5-1 mg/mL,among which palmitic acid showed significant antibacterial activity,especially to E. faecium(MIC of 0.25 mg/mL). 50,100 μg/mL lipid metabolites could signifiantly inhibit the release of inflammatory factor of NO and TNF-α in RAW264.7 in vitro. CONCLUSIONS:The lipid metabolites of endophytic fungus A. terreus from P. capitatum show anti- multi-drug resistant bacteria and anti-inflammatory effects.

17.
Article in Chinese | WPRIM | ID: wpr-852250

ABSTRACT

Objective To study the antibacterial mechanisms of ethyl acetate extract (B06e) from the fermentation liquid of an endophytic fungus Alternaria spp. Alternaria spp isolated from the medicinal plant Humata tyermanni. Methods Double dilution method was adopted to measure the minimum inhibitory concentration (MIC) of B06e against Escherchia coli. Then, the changes of electric conductivity of bacterial culture, nucleic acid and protein concentration before and after treated by B06e were analysed, respectively. Besides, flow cytometry, scanning electron microscopy, gel retardation experiments, circular dichroism spectrum and Real-time quantitative PCR were introduced to study the antimicrobial mechanisms of B06e against E. coli. Results The results showed that MIC value of B06e against E. coli was 25 μg/mL. The electric conductivity of 3 × MIC treatment group was 1.01 times the value of the control group. The β-galactosidase activity of 3 × MIC treatment group was 11.6 times more than the value of the control group. Flow cytometry analysis showed that PI positive cells ratio of 3 × MIC treatment group was 286.5 times the value of the control group. Scanning electron microscopy showed that cell surface becomes rough after the treatment of B06e, a large number of cell membrane collapse. These results suggested that B06e can increase the permeability of cell membrane, destroy the integrity of cell membrane. The results of gel retardation experiments and circular dichroism spectrum applied that B06e can be inserted into DNA structure at particular position, however, can not cause DNA degradation. Real-time quantitative PCR results showed that the expressions of recA and recN genes were both up-regulated with the values of 2.9 and 3.7 times the value of the control group, respectively. This result suggested that B06e can destroy the DNA structure, which force E. coli to initiate SOS repair. Conclusion B06e can kill E. coli cell by destroying the cell membrane and damaging DNA structure.

18.
Article in Chinese | WPRIM | ID: wpr-852215

ABSTRACT

Objective To investigate the effect of ethyl acetate extract (B06e) from fermentation liquid of an endophytic fungus Alternaria spp. on the cell membrane integrity and the permeability of Staphylococcus aureus. Methods The minimum inhibitory concentration (MIC) of B06e against S. aureus was measured by double dilution method; The changes of electric conductivity of bacterial culture, A260 and A280 before and after treated by B06e were analyzed, respectively. Besides, the changes of cell membrane permeability before and after by B06e was measured by flow cytometry. The effect of B06e on the cell membrane structure was investigated by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). Results The results showed that MIC value of B06e against S. aureus was 50 μg/mL. The conductivity of 3 × MIC treatment group was 1.06 times of the value of the control group; after treatment of B06e, the values of A260 and A280 were significantly higher than those of the control group: The beta-galactosidase activity of 3 × MIC treatment group was 9.43 times more than the value of the control group; Flow cytometry analysis showed that 3 × MIC treatment group by propidium iodide (PI) staining of positive cells was 47.63 times more than the control group; SEM and FT-IR analysis showed that the structure of bacterial cell changed after B06e treatment. Conclusion B06e can kill S. aureus cell by increasing the permeability of its cell membrane and destroy cell membrane integrity.

19.
Article in Chinese | WPRIM | ID: wpr-852165

ABSTRACT

Objective Try to find the new biological compounds, the research on the chemical constituents in the fermentation products of an endophytic fungus Phomopsis fukushii had been carried out. Methods The chemical constituents in this fermentation products were isolated by silica gel, Sephadex LH-20 column chromatographies and RP-HPLC methods. Their structures were elucidated by using various spectroscopic techniques. Results Four pentylated diphenyl ethers (1-4) were isolated from this fermentation products, and the new compound (1) was evaluated for its anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) activity. Compounds 2-4 were identified as diorcinol C, diorcinol D, and diorcinol E. Conclusion Compounds 2-4 are isolated from the fermentation products of endophytic fungus Phomopsis fukushii for the first time. Compound 1 is a new compound named phomodiphenyl ether A and given the system name of 1-[4-(3-hydroxy-5-methylphenoxy)-2-methoxy-6-methylphenyl]-3-methylbut- 3-en-2-one. Compound 1 also shows strong anti-MRSA activity with MIC90 value of (54 ± 4) μg/mL. This valve is close to that of positive control, levofloxacin with MIC90 value of [≥ (56 ± 6) μg/mL].

20.
Article in Chinese | WPRIM | ID: wpr-851539

ABSTRACT

Objective To study the secondary metabolites of endophytic fungus Fusarium oxysporum form Paeonia ostii. Methods The fermentation liquor of the fungal strain F. oxysporum were isolated and purified using various chromatographic methods. The structures of the compounds were identified by spectrum analysis. Results Twenty-three compounds were isolated from the fungal and their structures were identified as (2S)-proline (1), (22E,24R)-ergosta-7,22-diene-3β,5α,6β-triol (2), cyclo-(S-Pro-S-Leu) (3), cyclo-(L-Ala-L-Pro) (4), cyclo-(Val-Pro) (5), cyclo-(R-Pro-S-Phe) (6), cyclo-(D-cis-Hyp-L-Phe) (7), cyclo-(trans-4-hydroxy- L-Pro-L-Phe) (8), cyclo-(Gla-Tyr) (9), cyclo-(trans-4-hydroxy-L-Pro-L-Leu) (10), L,L-cyclo-(Trp-Pro) (11), cyclo-(L-Leu-Gly) (12), N-(3-(1H-indol-3-yl)propyl)acetamide (13), indole-3-acetic acid (14), 2-piperidone (15), 2-pyrrolidinone (16), thymidine (17), (5S)-5-[(4-hydroxyphenyl)methyl]-2,4-imidazolidinedione (18), (3S,6S)-3-(butan-2-yl)-6-(1-hydroxyethyl) piperazine-2,5-dione (19), cyclo-(Ala-Leu) (20), cyclo-(S-Pro-S-Phe) (21), cyclo-(Tyr-Pro) (22), and cyclo-(L-Phe-L-Tyr) (23). Conclusion With the exception of compounds 2, 6, and 14, the other compounds are isolated from the fermentation liquor of the fungal strain F. oxysporum for the first time.

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