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Objective:To investigate the diagnostic values of human epididymis protein 4 (HE4), endothelial cell specific molecule-1 (ESM-1) and epidermal growth factor receptor (EGFR) for lung cancer.Methods:The clinical data of 90 patients with lung cancer and 50 patients with benign lung diseases diagnosed by the pathological examination in Tangshan People's Hospital from December 2019 to January 2021 were retrospectively analyzed, and 40 healthy physical examiners in the same period were selected as the controls. The serum HE4 levels were detected by electrochemiluminescence method. The serum ESM-1 and EGFR levels were tested by enzyme-linked immunosorbent assay. The differences in serum HE4, ESM-1 and EGFR levels between the three groups were compared; logistic regression analysis was used to screen out the effective indicators for the diagnosis of lung cancer and to construct a prediction model for the diagnosis of lung cancer. Using pathological diagnosis result as the gold standard, the receiver operating characteristic (ROC) curve was drawn, and the diagnostic efficacy of indicators for lung cancer was evaluated.Results:The levels of serum HE4 in lung cancer group, benign lung diseases group and healthy control group were 119.55 pmol/L (82.06 pmol/L, 189.00 pmol/L), 58.84 pmol/L (45.62 pmol/L, 69.41 pmol/L) and 42.67 pmol/L (37.09 pmol/L, 51.84 pmol/L), the levels of ESM-1 were 33.00 ng/ml (25.85 ng/ml, 47.40 ng/ml), 20.14 ng/ml (11.93 ng/ml, 28.90 ng/ml) and 15.39 ng/ml (11.84 ng/ml, 20.19 ng/ml), and the levels of EGFR were 46.60 pg/ml (37.45 pg/ml, 58.98 pg/ml), 32.77 pg/ml (26.27 pg/ml, 40.86 pg/ml) and 30.43 pg/ml (27.54 pg/ml, 35.75 pg/ml), and the differences in each indicator among the three groups were statistically significant (all P < 0.001). The levels of serum HE4, ESM-1 and EGFR in lung cancer group were higher than those in benign lung diseases group and healthy control group. In patients with lung cancer, logistic regression analysis was performed with HE4 (X 1), ESM-1 (X 2) and EGFR (X 3) as the independent variables and pathological diagnosis as the dependent variable, and a lung cancer prediction regression model was established: P = 0.171X 1+0.351X 2+0.184X 3-24.660. The accuracy of this model in predicting lung cancer could reach 98.5%, and serum HE4, ESM-1 and EGFR were risk factors for the occurrence of lung cancer (all P < 0.05). The area under ROC curve from high to low was HE4 (0.960), ESM-1 (0.942) and EGFR (0.859). The diagnostic sensitivity of serum HE4 63.67 pmol/L for lung cancer was 86.7%, and the specificity was 97.5%. Both serum HE4 ( r = 0.304, P = 0.004) and ESM-1 ( r = 0.416, P < 0.001) were correlated with EGFR. Conclusions:Serum HE4, ESM-1 and EGFR can be used as effective indicators for the diagnosis of lung cancer, and the prediction model established based on the three serum tumor markers is of good value for the diagnosis and prediction of lung cancer.
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Objective:To investigate the serum levels of soluble low-density lipoprotein receptor 11 (sLR11), endothelial cell-specific molecule-1 (ESM-1), and advanced glycosylation end products (AGE) in patients with preeclampsia (PE) and their ability to predict adverse pregnancy outcomes.Methods:A total of 141 PE patients (PE group) and 60 normal pregnant women (control group) who were admitted to the Zhangjiakou Maternal and Child Health Hospital from January 2020 to October 2022 were selected. Serum levels of sLR11, ESM-1, and AGE were detected in each group. PE patients were divided into mild preeclampsia (MP, n=78) and severe preeclampsia (SP, n=63) according to the severity of the disease. PE patients were also divided into an adverse pregnancy outcome group ( n=57) and a good pregnancy outcome group ( n=84) based on the occurrence of adverse pregnancy outcomes. Receiver operating characteristic (ROC) curve analysis was used to evaluate the ability of serum levels of sLR11, ESM-1, and AGE to predict adverse pregnancy outcomes in PE patients. Pearson correlation analysis was used to examine the correlation between serum levels of sLR11, ESM-1, and AGE in PE patients. Results:Serum levels of sLR11, ESM-1, and AGE were significantly higher in the PE group than in the control group (all P<0.001). Serum levels of sLR11, ESM-1, and AGE were significantly higher in the SP group than in the MP group (all P<0.001). Serum levels of sLR11, ESM-1, and AGE were significantly higher in the adverse pregnancy outcome group than in the good pregnancy outcome group (all P<0.001). ROC curve analysis showed that the combination of sLR11≥11.65 μg/L, ESM-1≥2.14 μg/L, and AGE≥57.38 ng/ml had the largest area under the curve (AUC) for predicting adverse pregnancy outcomes in PE patients (0.947, 95% CI: 0.890-0.995), with a sensitivity of 97.3% and specificity of 82.0%. Pearson correlation analysis showed that serum levels of sLR11, ESM-1, and AGE were positively correlated in PE patients (all P<0.001). Conclusions:Serum levels of sLR11, ESM-1, and AGE are significantly increased in PE patients and are closely related to disease severity. The combination of these three factors has good value in predicting adverse pregnancy outcomes in PE patients.
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Objective This study was to evaluate the values of endothelial cell-specific moleculel (endocan),von Willebrand factor (vWF),and"A disintegrin-like and metalloprotease with thrombospondin type 1 motit"(ADAMTS-13),alone or in combination,in the risk stratification and disease severity assessment of patients with sepsis via comparing the differences of these markers in patients with systemic inflammatory reaction syndrome (SIRS),sepsis,severe sepsis,or septic shock,and healthy volunteers.Methods Clinical data of 301 patients with SIRS or sepsis treated in our Emergency Department from October 2014 to October 2015,were prospectively analyzed.These patients were divided into SIRS,sepsis,severe sepsis,and septic shock groups.40 healthy individuals were selected as control.Endocan,vWF,ADAMTS-13,vWF/ADAMTS-13,and Procalcitonin (PCT) levels were measured,and APACHE Ⅱ score,MEDS score as well as SOFA score were calculated.The all-cause death or survival of each patient was recorded during the 28-day follow-up.Results The endocan,vWF,and vWF/ADAMTS-13 levels significantly increased in patients with SIRS,sepsis,severe sepsis,or septic shock and were positively correlated with disease severity,and were also positively correlated with MEDS score,APACHE Ⅱ score,SOFA score.On the other way,the ADAMTS-13 level gradually declined during disease progression and was negatively correlated with the disease severity,it was also negatively correlated with MEDS score,APACHE Ⅱ score,SOFA score.Conclusions Endocan,vWF,ADAMTS-13,and vWF/ADAMTS-13 ratio are valuable in the risk stratification and disease severity evaluation of sepsis,providing novel sepsis biomarkers in clinic.
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Objective To explore the expression of endothelial cell specific molecule-1 (ESM-1) and its clinical significance in pituitary null cell adenoma (NCA).Methods Forty-six cases of NCA specimens,collected from resection of pituitary tumor via microscopic transsphenoidal approach in our hospital from January 2010 to May 2014,were chosen in our study;ESM-1 expressions in the NCA specimens was detected by immunohistochemical S-P method,and the relations of expression results with gender (male:29;female:17),age (18-45 years old:16;≥46 years old:30),maximum tumor diameter (1-3 cm:34;≥3 cm:11;＜1 cm:1),invasion state (invasive NCA:30;non-invasive NCA:16),Ki-67 labeling index (＜3％:29;≥3％:17) and pathological features were analyzed.Results ESM-1 expression was observed in endothelial cells and adenoma cells,with positive rates 100％ and 86.96％ (40/46);in the endothelial cells,high expression was noted in 32 patients;and in the adenoma cells,high expression was noted in 25 patients;no significant difference of high expression was noted between the two (P＞0.05).As compared with that ofnoninvasive NCA (7/16),the high expression of ESM-1 of invasive NCA (23/30) in endothelial cells was significantly increased (P＜0.05),while the high expression of ESM-1 in adenoma cells was not significantly increased (P＞0.05).In endothelial cells,high expression of ESM-1 was not significantly different in patients of different ages,gender,maximum tumor diameters or Ki-67 labeling indexes (P＞0.05);in adenoma cells,high expression of ESM-1 was not significantly different in patients of different ages,gender,maximum tumor diameters,Ki-67 labeling indexes or invasion states (P＞0.05).Conclusion ESM-1 in endothelial cells could be used as a marker of aggressive behavior in NCA.
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BACKGROUND: Endocan, previously called endothelial cell–specific molecule-1, is a soluble proteoglycan that is secreted from vascular endothelial cells. Elevated plasma endocan levels were shown to be associated with poor cardiovascular outcomes in patients with chronic kidney disease (CKD). We investigated the clinical relevance of plasma and urine endocan levels in patients with immunoglobulin A nephropathy (IgAN). METHODS: Sixty-four patients with IgAN and 20 healthy controls were enrolled in this study. Plasma and urine endocan levels were measured. Clinical parameters, pathologic grades, and renal outcomes were compared among subgroups with different plasma and urine endocan levels. RESULTS: Both plasma and urine endocan levels were significantly higher in patients with IgAN than in controls. Elevated serum phosphorus and C-reactive protein were independent determinants for plasma endocan, and elevated C-reactive protein was also an independent determinant for urine endocan levels in multivariate analysis. Plasma endocan level was not significantly different across CKD stages, but patients with higher plasma endocan levels showed adverse renal outcome. Urine endocan levels were also elevated in patients with poor renal function. Cox proportional hazard models showed that high plasma endocan was an independent risk factor for CKD progression after adjusting for the well-known predictors of outcome in patients with IgAN. CONCLUSION: This study suggested that plasma endocan might be useful as a prognostic factor in patients with IgAN.
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Humans , C-Reactive Protein , Endothelial Cells , Glomerulonephritis, IGA , Immunoglobulin A , Immunoglobulins , Multivariate Analysis , Phosphorus , Plasma , Prognosis , Proportional Hazards Models , Proteoglycans , Renal Insufficiency, Chronic , Risk FactorsABSTRACT
Objective To investigate the dynamic changes in endothelial cell specific molecule-1(ESM-1) in rat with sepsis and the relationship between ESM-1 level and renal injury.Methods Thirty-six SD rats were randomly divided into six groups: normal control group(n=6),sham operation group(n=6);CLP group(n=24),and in the latter group rats were divided into four subgroups according to time after CLP: CLP3h,CLP6h,CLP12h and CLP24h group(6 each).In rats of CLP group cecal ligation and puncture(CLP) was done to produce sepsis,and rats in sham operation group underwent the same surgical procedure as CLP group except the cesum was not ligated and punctured.The changes in ESM-1,blood urea nitrogen(BUN) and creatinine(Cr) level in peripheral blood were determined.Paraffin section and HE staining were used to examine pathological changes in kidney tissue,and immunohistochemisty was used to determine the ESM-1 expression in kidney tissue.Results Compared with normal control group,the serum level of ESM-1 increased significantly in CLP6h,CLP12h and CLP24h group(P