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1.
Article in English | WPRIM | ID: wpr-881068

ABSTRACT

Angelicae Sinensis Radix (Danggui) and Ligusticum Chuanxiong Rhizoma (Chuan Xiong) herb-pair (DC) have been frequently used in Traditional Chinese medicine (TCM) prescriptions for hundreds of years to prevent vascular diseases and alleviate pain. However, the mechanism of DC herb-pair in the prevention of liver fibrosis development was still unclear. In the present study, the effects and mechanisms of DC herb-pair on liver fibrosis were examined using network pharmacology and mouse fibrotic model. Based on the network pharmacological analysis of 13 bioactive ingredients found in DC, a total of 46 targets and 71 pathways related to anti-fibrosis effects were obtained, which was associated with mitogen-activated protein kinase (MAPK) signal pathway, hepatic inflammation and fibrotic response. Furthermore, this hypothesis was verified using carbon tetrachloride (CCl

2.
Article in Chinese | WPRIM | ID: wpr-873117

ABSTRACT

Coptidis Rhizoma-Evodia Fructus is a classic herb pair in traditional Chinese medicine prescriptions, the famous prescription is called Zuojinwan, which comes from Danxi Xinfa, is composed of Coptidis Rhizoma-Evodia Fructus (6∶1). In this formula, Coptidis Rhizoma has the effect of clearing heat and drying diuresis, purging fire to remove toxin and clearing heart. Evodia Fructus has the effects of expelling cold and alleviating pain, checking upward adverse flow of Qi tostop vomiting, and assisting yang to stop diarrhea. Coptidis Rhizoma has the properties of bitter and cold, and Evodia Fructus has the properties of pungent and calidus. Pungent drugs have divergent effects, and bitter drugs have sedimentation effect, when used in combination, they can clear the liver and purge fire, calm the adverse-rising energy and stop vomiting. On the basis of Zuojinwan, Coptidis Rhizoma-Evodia Fructus medicine has derived different compatibility ratios. Different ratios are different in terms of efficacy, usage, clinical application. Although with the application of modern analytical instruments and the development of molecular pharmacology theory, the chemical constituents and Pharmacological effects of Coptidis Rhizoma and Evodia Fructus have been fully studied, as to the principle of compatibility, and the study of pharmacological effects and chemical constituents after the compatibility of the two drugs in different proportions, there is still no comprehensive system summary. This article makes a systematic and comprehensive explanation of Coptidis Rhizoma-Evodia Fructus from the aspects of famous literature, chemical composition, pharmacological effects and clinical applicationthrough querying literature and ancient books. In order to make this herb pair more standardized, and provide reference materials for further research and development for this herb pair.

3.
Chinese Pharmaceutical Journal ; (24): 715-721, 2020.
Article in Chinese | WPRIM | ID: wpr-857718

ABSTRACT

OBJECTIVE: To investigate the absorption mechanism of paeoniflorin in Radix Paeoniae Alba, both of Radix Paeoniae Alba and Radix Angelica Sinensis and Siwu Decoction. METHODS: The circulatory perfusion technique was used in this study, the concentrations of paeoniflorin and phenol red in intestinal absorption circulation were respectively determined by HPLC and UV. The effects of pH value, drug concentration, absorption site and P-gp on the absorption of paeoniflorin were investigated separately. RESULTS: By comparing the absorption of paeoniflorin in Radix Paeoniae Alba, both of Radix Paeoniae Alba and Radix Angelica Sinensis, and Siwu Decoction, it was found that in the sample solution at the same absorption site, pH value and concentration (based on the concentration of paeoniflorin), the absorption, Ka and cumulative absorption of paeoniflorin in compound Siwu decoction group were significantly increased compared with the other groups (P<0.05), while t1/2 was significantly decreased (P<0.05). When combined with the inhibitor and inducer of P-gp, the absorption of paeoniflorin showed significant increase or decrease in the amount of absorption, Ka and cumulative absorption% compared with the control group (P<0.05), and t1/2 also showed significant decrease or increase (P<0.05). CONCLUSION: The absorption of paeoniflorin could be affected by P-gp, and under the same conditions, the absorption of paeoniflorin in complicated Chinese herbal formula is better than that in the single herb and herb-pair.

4.
Article in Chinese | WPRIM | ID: wpr-823994

ABSTRACT

Wutou-Gancao herb-pair is extensively used to attenuate the toxicity and enhance the efficacy of aconite. In this study, potential synergic mechanism of the herb pair was investigated by utilizing multiple ap-proaches. In silico and in vitro Caco-2 cell models were applied to study the potential binding mode of bioactive ingredients existing in liquorice with P-glycoprotein (P-gp), as well as the inhibition effects on P-gp. Additionally, anti-inflammatory activity of aconitine (AC) combined with active ingredients of liquorice, as well as pharmacokinetic patterns of AC after co-administration was investigated. Anti-inflammatory effect of AC (1 mg/kg) in rats was enhanced in combination with bioactive ingredients of liquorice (10 mg/kg). In the meanwhile, the exposure of AC in vivo was altered, in terms of Cmax and AUC. For instance, the Cmax and AUC were increased to 1.9 and 1.3 folds, respectively, when used in combination with liquiritigenin. The in silico study revealed the potential binding mode with outward facing conformation of P-gp. The resulting data obtained from transport of rhodamine-123 (Rh-123) across Caco-2 cell monolayer further indicated that the function of P-gp was inhibited by chemicals in liquorice. The synergic effect was therefore proposed to be attributed to inhibition of P-gp by liquorice since AC has been demonstrated to be the substrate of P-gp. The resuls revealed that potential synergic mechanism of Wutou-Gancao herb-pair by inhibiting function of key efflux transporter P-gp to enhance the exposure of AC in systematic circulation, and further the anti-inflammatory effect, which helps clarify the compatibility rationale of these two herbs.

5.
Acta Pharmaceutica Sinica ; (12): 941-949, 2020.
Article in Chinese | WPRIM | ID: wpr-821670

ABSTRACT

This study analyzed the effect of the Radix Bupleuri-Radix Paeoniae Alba herb pair on endogenous metabolites in rats with chronic unpredictable mild stress (CUMS)-induced depression by using LC-MS liver metabolomics. Rats were randomly divided into 5 groups: a normal control group, a CUMS model group, a venlafaxine-positive group, and a high-low dose group for the Radix Bupleuri-Radix Paeoniae Alba herb pair, with continuous modeling and administration over 28 days. The efficacy of Radix Bupleuri-Radix Paeoniae Alba herb pair was evaluated by measuring traditional pharmacodynamic indicators of depression (body weight, open field test, sucrose preference test and forced swimming test). Animal experimentation was approved by the Committee on the Ethics of Animal Experiments of Shanxi University (SXULL2016036). Liver metabolic profiles were obtained by the UHPLC-Q Exactive Orbitrap-MS metabolomics technique. The results show that the Radix Bupleuri-Radix Paeoniae Alba herb pair can significantly decrease depression-like behavior of rats in the CUMS model group. Increases in 25 depression-related metabolites were identified by LC-MS metabonomics, and the Radix Bupleuri-Radix Paeoniae Alba herb pair could significantly decrease 16 of them. Metabolic pathway analysis showed that D-glutamine and D-glutamate metabolism, arginine and proline metabolism, alanine, aspartate and glutamate metabolism, and glutathione metabolism were the main metabolic pathways altered by this herb pair in CUMS model rats.

6.
Acta Pharmaceutica Sinica ; (12): 235-244, 2019.
Article in Chinese | WPRIM | ID: wpr-780354

ABSTRACT

@#Depression, a global disease with various pathogenic factors, is seriously affecting human physical and mental health. In Chinese traditional medical theory, the disease belongs to the category of "Yu Zheng". At present, on the one hand, adverse reactions to antidepressant western medicine are becoming more and more prominent. On the other hand, the study of their antidepressant active ingredients and compatibility mechanisms has become a difficult field in the traditional Chinese medicine compounds due to their complexity. The Chinese antidepressant herb-pairs has become a hot topic in the field of antidepressant research. Herb-pair is the core of traditional Chinese medicine compound. In addition, the compound itself is a herb-pair. The study of traditional Chinese antidepressant herb-pairs is more conducive to clarify the compatibility mechanism of herb interaction and the mechanism of antidepressants on the body.Therefore, this paper systematically expounds antidepressant herb-pairs from the study of material, pharmacokinetics and efficacy, which aims at providing theoretical support for the compatibility mechanism of antidepressant herb-pairs and the research of new antidepressant drugs.

7.
Acta Pharmaceutica Sinica ; (12): 1909-1917, 2019.
Article in Chinese | WPRIM | ID: wpr-780274

ABSTRACT

We evaluated the effects of Danggui-Chuanxiong (GX) herb pair with different proportions (1∶0, 3∶2, 1∶1, 2∶3, 0∶1) and preparation methods (water extract W, alcohol extract A, and water-alcohol extracts WA) on vasoactive substances and endothelial cell adhesion molecules in the serum of acute blood stasis in rats. An acute blood stasis model was co-replicated by ice water bath and subcutaneous injection of epinephrine hydrochloride in rats. The expressions of vasoactive substances (arachidonic acid metabolites, coagulation-fibrin system index) and adhesion molecules in the serum were detected by enzyme linked immunosorbent assay method; the Spearman method was used to analyze the correlation of those detection indicators; the partial least squares-discriminant analysis and multi-attribute comprehensive index method were used to comprehensively evaluate the total effect of GX herb pair samples with different proportions and preparation methods on vasoactive substances and adhesion molecules. The experimental scheme was approved by the Animal Experimental Ethics Committee of the First Affiliated Hospital of Henan University of Chinese Medicine. The results showed that GX 1∶1_WA had the strongest effect on the improvement of vasoactive substances and adhesion molecules in the serum of acute blood stasis in rats (the total effect value was 6.96). When extraction method was same, the overall effect of GX 1∶1 had better effect than that of other proportions; when the proportion of GX was same, the total effects of GX_WA and GX_A were better than GX_W. The combination of Danggui and Chuanxiong can significantly improve the expressions of vasoactive substances and adhesion molecules in the serum of blood stasis in rats. But the action strength of GX herb pairs was different when the proportions and preparations of GX herb pair were different. These findings provide a basis for clinical rational application of GX herb pair, and lay the foundation for in-depth research on GX herb pair for treatment of blood stasis related diseases.

8.
Acta Pharmaceutica Sinica ; (12): 1564-1573, 2019.
Article in Chinese | WPRIM | ID: wpr-780263

ABSTRACT

The herb pair is the simple and exquisite experience summary accumulated by generations of traditional Chinese medicine (TCM) experts in the process of long-term herbal prescription for curing diseases. Although it is only a combination of two herbs, it is cleverly matched and properly proportioned, which can well interpret TCM characteristics of "great combination and artful application". Moreover, the herb pair is an intermediate point between single herb and several TCM formulae, which implicates certain regularity and some connotation of many TCM formulae for diagnosis and treatment based on an overall analysis of the illness and the patient's condition. Our team has long been focusing on the complexity of drug interactions and the diversity of TCM components to carry out systematic research on herb pair by using modern scientific and technological knowledge and approaches. As results, a series of modern research approaches and strategies formed for the compatibility effect and bio-active components of the herb pair. By representing the principles and application characteristics of these approaches, this paper provides important support for elucidating scientific connotation of compatibility regularity of herb pairs and application of formulae optimization, as well as explorative ideas and approaches for modern research on other herb pairs.

9.
Chinese Pharmacological Bulletin ; (12): 1020-1024, 2019.
Article in Chinese | WPRIM | ID: wpr-857214

ABSTRACT

Aim To explore the effects of Zhimu-Huangbai herb pair( ZB) on insulin resistance. Methods The rat model of experimental T2DM was induced by intraperitoneal injection of streptozotocin after feeding with high fat and sucrose forage. Rats with T2DM were randomly divided into normal group, model group, metformin group and high-, low-dose ZB group(4. 5, 1. 5 g • kg"1) , with corresponding drugs administered orally by gastric gavage once a day for four weeks. The body weight, fasting blood glucose ( FBG ) , serum insulin level ( FINS ) and triglyceride content(TG) were measured, and the insulin sensitivity index(ISI) and insulin resistance index ( Homa-IR) were calculated. The HepG2 cells were treated with palmitate and oleate to induce insulin resistance, then divided into six groups: Control group, model group, metformin group and high-, middle-, low-dose ZB group(384, 192, 96 mg • L'1). Glucose consumption and TG content were assayed 24 h after administration. Results ZB not only markedly lowered the level of FBG, FINS, TG content (P <0. 05) and insulin resistance index ( P < 0. 01 ) , increased insulin sensitivity index(P <0. 05) in rats with T2DM, but also significantly decreased TG content (P <0. 05) and increased glucose consumption ( P < 0. 05 ) in HepG2 cell model with insulin resistance. Conclusions ZB can promote glucolipid metabolism and improve the insulin resistance by increasing insulin sensitivity.

10.
Article in Chinese | WPRIM | ID: wpr-851158

ABSTRACT

Objective To study the functional material basis of herb pair Paeoniae Radix Alba-Glycyrrhizae Radix et Rhizoma and analyze their mechanism of action by the systematic pharmacological method based on the platform of traditional Chinese medicine systematic pharmacological analysis (TCMSP) and the big data of traditional Chinese medicine. Methods TCMSP was used to search for the chemical constituents of the two Chinese herbal medicines, “Baishao” and “Gancao”. The molecular descriptors of the components were compared by the two tailed Mann-Whitney U test method, and the oral bioavailability (OB) and drug-likeness (DL) were used as indicators to screen the active components, targets and related diseases of the herb pair. The “component-target-disease” and PPI network models were constructed, and bioprocess and metabolic pathway were analyzed to explore the drug-based material basis and mechanism of action. Results Through the OB and DL parameters screening, 49 active pharmaceutical ingredients, 100 targets and 230 related diseases were obtained. The active ingredients with higher degree were formononetin, naringenin, and vestitol; The higher degree of targets were prostaglandin g/H synthase 2 (PTGS2), estrogen receptor (ESR1), and calmodulin (CALM); The higher-grade related diseases were unspecific cancer, inflammation, cardiovascular disease, unspecified, Alzheimer’s disease, and Parkinson’s disease, mainly involving 16 categories of diseases such as cancer, nervous system diseases, endocrine, nutritional and metabolic diseases and certain infectious diseases and parasitic diseases. Target proteins are involved in bioprocess including signal transduction, drug response, cell proliferation, RNA polymerase II promoter regulation, positive regulation of ERK1 and ERK2 cascades, regulating metabolic pathways such as PI3K-Akt and 5-hydroxytryptamine synapses. Conclusion This study preliminarily verified the basic pharmacological effects and mechanisms of the Paeoniae Radix Alba-Glycyrrhizae Radix et Rhizoma herb pair, and explored new ideas for the study of the herb pair and related traditional Chinese medicine prescriptions.

11.
Article in English | WPRIM | ID: wpr-691343

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the antipyretic mechanism of Herba Ephedrae (Eph)-Ramulus Cinnamomi (RC) herb pair on yeast-induced pyrexia in rats.</p><p><b>METHODS</b>Totally 30 qualified male SD rats were randomly assigned to the normal control (NC) group, the pyrexia model (model) group, the Eph, RC and Eph-RC treatment groups by a random digital table, 6 rats in each group. Each rat received a 20% aqueous suspension of yeast (10 mL/kg) except the NC group. The 3 treatment groups were administered 8.1, 5.4 and 13.5 g/kg Eph, RC and Eph-RC respectively at 5 and 12 h after yeast injection, the NC group and the model groups were administered equal volume of distilled water. Rectal temperatures were measured at 0, 6, 8, 10, 12, 15, 18, 24 and 30 h and urine was collected prior to yeast injection and at 6, 10, 18, 24, 30, and 36 h after yeast injection. Then urine metabolomic profiling by gas chromatography tandem mass spectrometry, coupled with multivariate statistical analysis and pattern recognition techniques were used to explore the antipyretic effects of Eph-RC. Partial least squares discriminate analysis was used to analyze the metabolomics dataset including classification and regression in metabolomics plot profiling.</p><p><b>RESULTS</b>Compared with the NC group, rectal temperatures were significantly higher in the model group (P<0.01), while 3 treatment groups decreased significantly compared with the model group (P<0.05 or P<0.01). Rectal temperatures of Eph-RC-treated rats started to go down at 6 h, and markedly decreased at 8, 12, 15, 18 and 24 h (P<0.05 or P<0.01), while those of the Eph and RC groups had decreased firstly at 8 h and were markedly lower at 12 h (P<0.05 or P<0.01). Seventeen potential biomarkers related to pyrexia were confirmed and identified, including pyruvic acid, L-phenylalanine, L-tyrosine, phenylacetic acid, hippuric acid, succinic acid, citrate and so on. Eight potential alterations of metabolic pathways including phenylalanine metabolism, citrate cycle, tryptophan metabolism, biosynthesis of valine, leucine and isoleucine, were identified in relation to the antipyretic effects of Eph-RC using MetPA software.</p><p><b>CONCLUSION</b>The antipyretic effect of Eph-RC herb pair on yeast-induced pyrexia in rats involved correction of perturbed amino acid, fatty acid, and carbohydrate metabolism according to the metabolic pathway analysis with MetPA.</p>

12.
Acta Pharmaceutica Sinica ; (12): 630-635, 2018.
Article in Chinese | WPRIM | ID: wpr-779917

ABSTRACT

The response surface method was used to evaluate the concentration range, property and degree of Scutellaria (HQ) and Coptis (HL) herb pair on improvement of glucose and lipid metabolism in this paper. Type 2 diabetes mellitus (T2DM) was induced in rats by low a dose of streptozotocin (STZ) in combination with high-fat diet feeding. The rats were administered with HQ-HL extract pairs at different ratio (1:0, 3:1, 2:1, 3:2, 1:1, 2:3, 1:2, 1:3, 0:1). To investigate the influence of herb pair extracts on glucose and lipid metabolism of T2DM rats, the multi index synthetic index method was adopted to integrate the indexes, then the response surface method was applied to analyze the integration results. The ratio of HQ and HL from 3:1 to 1:3 showed a synergistic effect (with the value between -0.2 and -0.8). Especially, when the proportion was between 3:2 and 1:3, herb pair extracts possessed stronger synergistic effects (value:-0.8). The high dose of HQ-HL with the ratio of 1:1 exhibited the strongest synergistic effect.

13.
Article in Chinese | WPRIM | ID: wpr-335870

ABSTRACT

Lifting and lowering theory is one of the important basis for guiding clinical medication. Through the study of ancient books and literature, we learned that lifting and lowering theory was originated in Huangdi Neijing, practiced more in the Shanghan Zabing Lun, established in Yixue Qiyuan, and developed in Compendium of Materia Medica and now. However, lifting and lowering theory is now mostly stagnated in the theoretical stage, with few experimental research. In the clinical study, the guiding role of lifting and lowering theory to prescriptions?mainly includes opposite?role?of lift and lower medicine property, mutual promotion of lift and lower medicine property, main role of lift medicine property and main role of lower medicine property. Under the guidance of lifting and lowering theory, the herb pair compatibility include herb combination of lift medicine property, herb combination of lift and lower medicine property and herb combination of lower medicine property. Modern biological technology was used in this study to carry out experimental research on the lifting and lowering theory, revealing the scientific connotation of it, which will help to promote clinical rational drug use.

14.
Article in Chinese | WPRIM | ID: wpr-275116

ABSTRACT

Herb pairs are usual clinical compatibility forms and one of compound prescription sources in Chinese medicine. Pharmacokinetic research in vivo is one of the important items in elucidating the mechanism for synergistic and attenuated mechanisms of herb pairs. The paper comprehensively summarized and systemized the pharmacokinetic researches of marker-ingredients about Danshen-Honghua and Danshen-Bingpian in order to elucidate the rationality and scientificity of herb pairs and provide some feasible suggestions on the pharmacokinetics of drugs in the future. In view of complicated system of Traditional Chinese medicines and a chemical system that is not separated from its natural state, comparative pharmacokinetic researches on marker-ingredients from the herb pairs are reasonable to elucidate the synergistic and attenuated mechanisms of monarch-subjects compatible herbs and monarch-guide compatible herbs. Such pharmacokinetic research can better explain the mechanism of drug compatibility, while the pharmacokinetic researches based on the monomer chemical compositions and marker-ingredients that have been separated from complex chemical environment of traditional Chinese Medicine are still unreasonable and should be discussed deeply.

15.
Article in Chinese | WPRIM | ID: wpr-613172

ABSTRACT

[Objective]The article summarizes the experience of Professor HE Lingzhi in treating purpura nephritis . [Methods]To collect and analyse the theory of HE's experience of herb pairs in treating purpura nephritis by studying from her for a long time and one medical record. [Results]Professor HE Lingzhi believes that the disease needs stage therapeutics and it is caused by pathogen wind pyretic pathogenic factor and poisonous evil in the stag of onset. Deficiency of both qi and yin in remission period. Syndrome of blood stasis consistently throughout purpura nephritis. Pro. HE treats purpura nephritis by pairing drugs,such as schizonepetae and honeysuckle, caulis sinomenii and saposhnikovia divaricata, astragalus membranaceus and eclipta alba, the root of red-rooted salvia and puccoon. [Conclusion] Professor HE's clinical experience in treating purpura nephritis from pairing drugs is well worth learning for it always gets outstanding effect.

16.
Article in Chinese | WPRIM | ID: wpr-696004

ABSTRACT

Xiaoqinglong decoction,first recorded by Zhang Zhongjing in traditional Chinese medicine (TCM) classic text Shang Han Lun (Treatise on Cold Damage Diseases),has eight herbal components including Herb Ephedrae (Mahuang),Radix Paeoniae Alba (Shaoyao),Radix Asari (Xixin),Rhizoma Zingiberis (Ganjiang),Glycyrrhiza Uralensis (Gancao),Ramulus Cinnamomi (Cuizhi),Rhizoma Pinelliae (Banxia) and Fructus Schisandrae (Wuweizi).Fingerprints of HPLC for single herb and herb-pair including Mahuang-Gancao,Mahuang-Guizhi,Shaoyao-Guizhi,Shaoyao-Gancao,Wuweizi-Xixin were analyzed.Based on the HPLC of single herb and herbal pair,we discussed the character of the Xiaoqinglong decoction and explored the scientific connotation of the compatibility of medicines in prescription.

17.
Chinese Herbal Medicines ; (4): 147-152, 2017.
Article in Chinese | WPRIM | ID: wpr-842189

ABSTRACT

Objective To investigate the metabolic routes and metabolites of Rehmannia glutinosa and Cornus officinalis herb pair produced by gut microbiome from rats. Methods A rapid and sensitive ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS) technique combined with Metabolynx™ software was established and successfully applied to identify the metabolites of the main bioactive components in the herb pair extract by rat intestinal bacteria. Results Four parent compounds (loganin, morroniside, catalpol, and acteoside) and their eight corresponding metabolites were detected and tentatively identified by the characteristics of their protonated ions. Hydrogenated and demethylated loganetin, dehydroxylated morronisid aglycone, caffeic acid, and its methylated product were the main metabolites. These metabolites suggested that the glycosides were firstly hydrolyzed to their aglycones by hydrolytic enzymes of the enteric microbial flora and subsequently to the other metabolites through hydrogenation, (de)-methylation, and de-hydroxylation. Conclusion The results may be helpful for the further investigation of the pharmacokinetic study of R. glutinosa and C. officinalis herb pair in vivo.

18.
Article in Chinese | WPRIM | ID: wpr-852788

ABSTRACT

Objective: To demonstrate the range, properties and extent of the interaction for activating blood effects in herb pair containing Angelicae Sinensis Radix and Carthami Flos (ASR-RC) by response surface method. Methods: Based on the acute blood stasis rats model, the hemorheological indexes of ASR-RC with different proportions (1:0, 4:1, 2:1, 3:2, 1:1, 2:3, 1:2, 1:4, 0:1) and different concentration were observed. Then all data were integrated to the total activating blood circulation effect value by comprehensive index method. The interaction was analyzed by response surface method. Model parameters were estimated with nonlinear regression. The three-dimensional response surfaces were constructed with Matlab software. Results The proportion from 0.8:1 to 1.1:1 of ASR-RC had synergetic effects, and when the dose of Angelicae Sinensis Radix ranged from 0 g to 5 g, and Carthami Flos from 8 g to 11 g, the herb pair also had synergetic effects (with the value between -0.2 and -0.8). In addition, when the dose of Angelicae Sinensis Radix ranged from 0 g to 0.2 g, and Carthami Flos ranged from 0.2 g to 1 g, the herb pair had antagonism action (with the value of 0.2). Conclusion The research results could provide scientific evidence for clinical application of ASR-RC. And quantitative analysis on drug interactions of herb pair by response surface method could also provide references for relevant studies.

19.
Article in Chinese | WPRIM | ID: wpr-852407

ABSTRACT

As the basic form of Chinese materia medica (CMM) prescriptions, herb pair established a bridge linking single herb and CMM formula. Studying the compatibility mechanism of herb pair has important significance for revealing the compatibility mechanism of CMM formula. At present, many researchers studied the changes of the material basis, pharmacokinetics, and pharmacological effects after herb pair combined as the breakthrough point to explore compatibility mechanism of the herbal pair. In this paper, the research status were summarized and put forward prospects of the compatibility mechanism of the herbal pair and the foundation for modern research were expected to lay on compatibility mechanism and development of new drug based on the herb pair.

20.
Article in Chinese | WPRIM | ID: wpr-852346

ABSTRACT

Objective To study the mechanism of attenuated Strychni Semen (SS) and Glycyrrhizae Radix et Rhizoma (GRR) compatibility with the phase angle as the starting point. Methods The phase separation of SS single decoction and compatibility decoction was carried out by natural sedimentation, dialysis and high-speed centrifugation. UPLC-MS assay was used to study the differences in the distribution of brucine and strychnine in four different phases, and the change of the amount of toxic substances was analyzed. Results The compatibility of the decoction can be divided into four groups: true solution group, colloidal solution group, suspension group, and sediment group. Brucine and strychnine were well separated under the selected chromatographic conditions. The linear relationship was good in the range of 0.020-2.008 and 0.022-2.236 μg (r = 0.999 8), and the recovery was 96.84% and 97.62%, which indicated that the method was good. The distribution of brucine and strychnine in the different phase of the single decoction showed the same variation regularity, colloid group > solution group > sediment group > suspended group; Compatibility decoction of different phase of brucine and strychnine also showed the same distribution, sediment group > colloidal group > suspension group > solution group. Conclusion The contents of toxic substances in SS were decreased after compatibility with GRR, especially for the strongest toxicity of strychnine, which mainly concentrated in the sediment components. Studies on the changes of toxic substances in phase states indicated that the sustained release effect of the sediments components after compatibility with GRR is the key factor of reducing toxicity and improving efficiency.

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