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1.
Chinese Journal of School Health ; (12): 472-475, 2022.
Article in Chinese | WPRIM | ID: wpr-923155

ABSTRACT

Abstract@#Allostatic load (AL) is related to stress. Adverse childhood experiences(ACEs), as a common stress in childhood, can make a serious and lasting impact on it. Allostatic load can reflect the wear and tear of an individual s physiological system. This article mainly reviews the functional changes of several systems of AL who have experienced ACEs, including neuroendocrine, metabolism, immune, and cardiovascular systems, as well as the different effects of the occurrence time and subtypes of ACES on AL, providing some theoretical basis for the development of early intervention plans in the future and reducing the occurrence and development of deleterious outcomes.

2.
Rev. bras. educ. méd ; 46(1): e027, 2022. tab, graf
Article in Portuguese | LILACS-Express | LILACS | ID: biblio-1365620

ABSTRACT

Resumo: Introdução: A orientação comunitária apresenta, em estudos de avaliação dos atributos da atenção primária à saúde, baixa performance entre os usuários. Questiona-se, em especial, se a qualificação profissional em Medicina de Família e Comunidade (MFC) contribui para a otimização desse desempenho. Objetivo: Este estudo teve como objetivo verificar a associação entre qualificação profissional em MFC e o grau de orientação comunitária na perspectiva dos profissionais médicos atuantes na Estratégia Saúde da Família de Belo Horizonte. Método: Trata-se de estudo censitário que reuniu médicos de todas as regionais de Belo Horizonte. Variáveis sociodemográficas relativas à formação acadêmica e à situação ocupacional foram obtidas por meio de questionário. Utilizou-se o instrumento PCATool-Brasil para medir o desempenho do atributo. Resultado: A comparação entre desempenho do escore orientação comunitária e variáveis independentes foi feita pelo teste qui-quadrado, com correção de Fischer. O escore médio foi de 7,9 (DP ±1,2). Nenhuma variável independente foi associada com a orientação comunitária satisfatória. Houve correlação entre tempo de conclusão da residência médica em MFC e alto escore para orientação comunitária. Conclusão: A qualificação em MFC não determina, por si só, um alto grau de orientação comunitária. O maior tempo de formação em MFC na modalidade residência médica correlacionou-se com maior desempenho desse atributo derivado.


Abstract: Introduction: In studies evaluating the attributes of Primary Health Care, Community Orientation has shown weak performance among users. In particular, questions remain over whether professional qualification in Family Practice (FP) helps optimize this performance. Purpose: verify the association between professional qualification in FP and the degree of Community Orientation from the perspective of medical professionals working in the Family Health Strategy of Belo Horizonte. Methods: This census study was applied to doctors in Belo Horizonte. Sociodemographic variables related to academic training and occupational status were obtained through a questionnaire. The PCATool-Brasil was used to measure the performance of this attribute. Result: The comparison between the performance of the Community Orientation score and independent variables was made using the chi-square test, with Fischer correction. The average score was 7.9 (SD ± 1.2). No independent variable was associated with a satisfactory level of Community Orientation. There was a correlation between time of completion of medical residency in FP and a high Community Orientation score. Conclusion: Qualification in FP does not itself determine a high degree of Community Orientation. Longer training in FP medical residency was correlated to better performance of this derived attribute.

3.
Barbarói ; (60): 232-258, jul.-dez. 2021. ilus
Article in Portuguese | LILACS, INDEXPSI | ID: biblio-1361861

ABSTRACT

Neste artigo, discute-se sobre os instrumentos e mecanismos de gestão para o controle social no campo da saúde do trabalhador e da trabalhadora, com foco na identificação e a utilização dessas ferramentas de gestão na atuação do Conselho Municipal de Saúde (CMS), da Comissão Intersetorial de Saúde do Trabalhador e da Trabalhadora (Cistt) e do Centro de Referência em Saúde do Trabalhador (Cerest) no contexto de Chapecó /SC. Para este estudo, ocupou-se da cartografia, que compreendeu observação participante, levantamento documental e realização de entrevista coletiva. A análise temática de conteúdo apoiou a análise de informações colhidas. O exercício do controle social está ancorado por instrumentos e mecanismos para a gestão em várias áreas de políticas pública. Os instrumentos de gestão compõem o ordenamento oficial, de base legal e legítima para orientar processos deliberativos e fiscalizatórios de instâncias participativas no âmbito público. Já os mecanismos compreendem modos e dinâmicas na condução de processos, ancorados pela participação social, pelos debates e decisões das ações e a fiscalização decorrente, salvaguardando os interesses e necessidades coletivos. Temas, instrumentos e mecanismos foram identificados na atuação do CMS, da Cistt e do Cerest. Muito embora, avanços importantes para o campo da saúde do trabalhador e da trabalhadora marcados desde a CFB de 1988, sobretudo, pelo conjunto de instrumentos e mecanismos integrados na atuação dessas instâncias em apoio à participação e ao exercício do controle social, vêm sofrendo reveses com a flexibilização e desregulamentação trabalhista impostas no Brasil nos tempos atuais.(AU)


This article discusses the management of instruments and mechanisms for social control in the field of workers' health, focusing on the identification and use of these management tools in the performance of the Municipal Health Council (CMS) of the Intersectoral Commission for Occupational Health (Cistt) and the Reference Center for Occupational Health (Cerest) in the context of Chapecó/SC. For this study, cartography was used, which included participant observation, documental survey and collective interview. Thematic content analysis supported it by collecting information. The exercise of social control is realized by instruments and mechanisms for management in various areas of public policy. The management instruments are part of the official order, with a legal and legitimate basis to guide deliberative and inspection processes of participative instances in the public sphere. Mechanisms, on the other hand, comprise modes and dynamics in conducting processes, supported by social participation, by debates, decisions of the actions and the elapsing inspection, safeguarding collective interests and needs. Themes, instruments and mechanisms were identified in the performance of the CMS, Cistt and Cerest. Even though, important advances in the field of the workers' health marked since the CFB of 1988, above all, by the set of instruments and mechanisms integrated in the performance of these instances in support of participation and the exercise of social control have suffered setbacks with the flexibilization and deregulation of labor imposed in Brazil today.(AU)


En este artículo se analizan los instrumentos de gestión y los mecanismos de control social en el ámbito de la salud del trabajador y de la trabajadora, centrándose en la identificación y uso de estas herramientas de gestión en el desempeño del Consejo Municipal de Salud (CMS) de la Comisión Intersectorial de Salud Ocupacional (Cistt). ) y el Centro de Referencia en Salud Ocupacional (Cerest) en el contexto de Chapecó/SC. Para este estudio se utilizó la cartografía, que incluyó observación participante, encuesta documental y entrevista colectiva. El análisis de contenido temático apoyó el análisis de la información recopilada. El ejercicio del control social está anclado en instrumentos y mecanismos de gestión en diversas áreas de la política pública. Los instrumentos de gestión conforman el orden oficial, con base legal y legítima para orientar los procesos deliberativos y fiscalizadores de las instancias participativas en la esfera pública. Los mecanismos, por su parte, comprenden modos y dinámicas en la conducción de los procesos, anclados en la participación social, los debates y decisiones sobre las acciones y la fiscalización resultante, resguardando los intereses y necesidades colectivos. Se identificaron temas, instrumentos y mecanismos en la actuación de la CMS, Cistt y Cerest. A pesar de importantes avances en el campo de la salud del trabajador marcados desde la CFB de 1988, sobre todo, por el conjunto de instrumentos y mecanismos integrados en el desempeño de estas instancias en apoyo de la participación y el ejercicio del control social, han sufrido retrocesos con la flexibilización y desregulación laboral impuesta en Brasil en la actualidad.(AU)


Subject(s)
Humans , Public Policy , Social Control, Formal , Occupational Health , Population Health Management
4.
Article in Portuguese | LILACS-Express | LILACS | ID: biblio-1353210

ABSTRACT

Objetivos: Comparar os critérios de formação de preços de medicamentos no Brasil e em países selecionados, analisar o mecanismo de formação de preços de medicamentos no Brasil e analisar o mecanismo de formação de preços de medicamentos em países selecionados. Métodos: Foi realizada uma revisão narrativa da literatura por meio do levantamento de informações em bases de dados, em sites das agências nacionais e organismos internacionais e em literatura "cinzenta", a respeito dos sistemas de saúde e mecanismos de formação de preços de medicamentos no Brasil e nos países selecionados (Austrália, Canadá, Espanha, Estados Unidos, França, Grécia, Itália, Nova Zelândia e Portugal). Resultados: A maioria dos países pesquisados utiliza o referenciamento externo e interno de preços, realiza ajustes e correções de preços ao longo do tempo e faz estudos de avaliação econômica. O valor da terapia ou seu benefício para o paciente ou sistema de saúde é um fator importante tanto na determinação do preço como da incorporação no sistema de saúde. Conclusão: Este trabalho permitiu identificar as semelhanças entre as práticas recomendadas e implementadas internacionalmente e as realizadas no Brasil, bem como os problemas relacionados à definição de preços das novas terapias, além das lacunas no modelo regulatório atual


Objectives: To compare the criteria for setting prices of medicines in Brazil and in selected countries, to analyze the mechanism for setting prices of medicines in Brazil and to analyze the mechanism for setting prices of medicines in selected countries. Methods: A narrative review of the literature was carried out by collecting information in databases, on websites of national agencies and international organizations and in "gray" literature, regarding health systems and price formation mechanisms of medicines in Brazil and selected countries (Australia, Canada, Spain, United States, France, Greece, Italy, New Zealand and Portugal). Results: Most of the countries surveyed use external and internal price referencing, make price adjustments and corrections over time and carry out economic evaluation studies. The value of therapy or its benefit to the patient or health care system is an important factor in both pricing and incorporation into the health care system. Conclusion: This work allowed identify the similarities between the practices recommended and implemented internationally and those carried out in Brazil, as well as the problems related to the pricing of new therapies, in addition to the gaps in the current regulatory model

5.
Rev. Ciênc. Plur ; 7(3): 158-179, set. 2021. ilus
Article in Portuguese | LILACS, BBO | ID: biblio-1343520

ABSTRACT

Introdução:Em saúde pública deve-se considerar dados epidemiológicos e métodos de avaliação da assistência com o intuito de promover melhoria. Nesta perspectiva, há os modelos lógicos que associam atividades, entradas de recursos, saídas de produtos e resultados. Objetivo:Construir um modelo lógico ilustrativo do funcionamento de uma Unidade de Saúde da Família e validar o seu conteúdo. Metodologia:Foi realizada revisão de literatura, observação participante e conversa com informantes-chave. Ademais, houve validação do conteúdo por profissionais atuantes na Unidade de Saúde da Famíliaem uma roda de conversa. Resultados:Elaborou-se cinco dimensões (Gestão do Trabalho, Atividades não assistenciais, Atividades assistenciais, Educação permanente continuada e Atividades docentes assistenciais) relacionadas à missão e aos elementos de recursos (físicos, informação, parcerias e financeiros), caracterizando, assim, o modelo. Conclusões:Os impactos alcançados com a aplicação do modelo remetem a critérios de qualidade (legitimidade, empoderamento e resolutividade); ao modelo assistencial instituído (vigilância à saúde); a características do próprio modelo (Clínica ampliada, intersetorialidade, ações multiprofissionais), além da motivação profissional. Todavia, embora validado por Unidade de Saúde da Famíliaespecífica, o modelo orienta-se por uma Política Nacional, podendo contribuir para outras Unidades de Saúde da Família de diferentes localidades (AU).


Introduction:In public health, in order to improve quality, epidemiological data and methods for evaluation of care should be considered. Logical models that associate activities, expenditure of resources, output of products, and results can be a useful assessment tool. Objective:To designand validate a logical model representingthe operationof a Family HealthcareUnit. Methodology:A literature review was initially performed, followed by participant observation and conversation with key informants. In addition, model was validated by professionals working at the Family HealthcareUnit (FHU) in a conversation roundtable. Results:Five dimensions were conceived: Work management;Noncare-related activities;Care-related activities; Permanent education;and In-service teaching, which relate to the mission of the service and the relevant resources (physical, information, partnerships and financial), thus constituting the model. Conclusions:The impacts achieved with the application of the model concernthe quality criteria (legitimacy, empowerment andproblem-solving capacity); the instituted healthcare model (active surveillance); features of the model itself (extended clinic, intersectoriality, multiprofessional actions); as well as professional motivation. Although themodel was validated only in a local Health Center, it follows a National Policy and may contribute to quality improvement of other Health Centersfrom different areas (AU).


Introducción: En salud pública, se deben considerar los datos epidemiológicos y los métodos de evaluación de la atención para promover la mejora. En esta perspectiva, existen modelos lógicos que asocian actividades, insumos de recursos, productos y resultados.Objetivo: Construir un modelo lógico que ilustre el funcionamiento de una unidad de salud familiar y validar su contenido. Metodología: Se realizó una revisión de la literatura, observación participante y conversación con informantes clave. Además, el contenido fue validado por profesionales que trabajan en la Unidad de Salud Familiar (FHU) en un círculo de conversación. Resultados: Se elaboraron cinco dimensiones (gestión del trabajo, actividades de no asistencia, actividades de asistencia, educación continua y actividades de asistencia a la enseñanza) relacionadas con la misión y los elementos de los recursos (físicos, de información, asociaciones y financieros), caracterizando, así, la modelo. Conclusiones: Los impactos logrados con la aplicación del modelo se refieren a criterios de calidad (legitimidad, empoderamiento y resolución); el modelo de atención establecido (vigilancia de la salud); las características del modelo en sí (clínica ampliada, intersectorialidad, acciones multiprofesionales), además de la motivación profesional. Sin embargo, aunque validado por una Centro de Saludespecífica, el modelo está guiado por una Política Nacional y puede contribuir a otrasCentros de Saludem diferentes ubicaciones (AU).


Subject(s)
Health Evaluation , Family Health , Health Care Evaluation Mechanisms , Family Health Strategy , Health Services Research/methods , Brazil/epidemiology , Qualitative Research , Evaluation Studies as Topic
6.
Rev. cuba. invest. bioméd ; 40(2): e808, 2021.
Article in Spanish | LILACS, CUMED | ID: biblio-1347463

ABSTRACT

Introducción: La COVID-19 es una enfermedad relativamente nueva, que puede causar afecciones en diferentes órganos. El daño renal se asocia a la mortalidad, por lo que debe ser identificado precozmente. Objetivo: Describir el daño renal en pacientes de COVID-19. Métodos: Se realizó una revisión bibliográfica de materiales publicados durante el periodo comprendido entre el 20 de abril al 25 de agosto de 2020. Se consultaron las bases de datos PubMed, SciELO, Ebsco y Clinical Key, así como el motor de búsqueda Google Académico. Los métodos utilizados fueron análisis-síntesis, inducción-deducción e histórico-lógico. Análisis e integración de la información: Para realizar un diagnóstico precoz que permita modificar el pronóstico y la historia natural de la mortalidad, se realizan numerosas investigaciones en los pacientes portadores de la COVID-19. En la literatura científica se reportan artículos sobre la importancia de la detección del daño renal. Se ha identificado que, tanto el daño renal previo, como el desarrollo de este durante la COVID-19, desempeñan un rol importante en el aumento de la tasa de letalidad. Conclusiones: La Afección renal crónica es una agravante en pacientes que padecen COVID-19. La descripción de los mecanismos causantes de la lesión renal es fundamental para establecer el pronóstico de estos pacientes, sin embargo, luego de la revisión, se comprobó que aún es escasa la literatura científica que aborde la relación del daño renal durante la COVID-19(AU)


Introduction: COVID-19 is a relatively new disease that affects various organs. Associated as it is to mortality, kidney damage should be identified as early as possible. Objective: Conduct a bibliographic review aimed at describing kidney damage in COVID-19 patients. Methods: A bibliographic review was conducted of materials about the topic published from 20 April to 25 August 2020. The search was carried out in the databases PubMed, SciELO, Ebsco and Clinical Key, as well as the search engine Google Scholar. Analytic-synthetic, inductive-deductive and historical-logical methods were used. Data analysis and integration: In order to obtain an early diagnosis allowing to modify the prognosis and natural evolution of mortality, a large number of studies are performed on patients carrying COVID-19. Papers are found in the scientific literature which refer to the importance of detecting kidney damage. It has been found that kidney damage, either developing before or during the course of the disease, plays an important role in the increase in lethality. Conclusions: Kidney damage is common in COVID-19 patients. Describing the mechanisms causing the kidney injury is a fundamental pillar to establish the prognosis for these patients. The description of kidney damage during COVID-19 is still scarce in the scientific literature(AU)


Subject(s)
Humans , Play and Playthings , Early Diagnosis , Data Analysis , Renal Insufficiency, Chronic/complications , COVID-19/complications
7.
Rev. colomb. biotecnol ; 23(1): 6-16, ene.-jun. 2021. tab, graf
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1289176

ABSTRACT

RESUMEN Las rizobacterias forman parte de la gran cantidad de microorganismos que actúan como agentes de biocontrol, produciendo metabolitos que inducen resistencia sistémica en las plantas que inhiben el crecimiento de patógenos. El objetivo de esta investigación fue evaluar la capacidad de diez rizobacterias de los géneros Rhizobium, Bradyrhizobium, Sinorhizobium, Ochrobactrum y Pseudomonas para producir ácido cianhídrico (HCN), sideróforos y ácido indol-acético (AIA), disolver fosfato, fijar nitrógeno e inhibir el crecimiento de fitopatógenos. Se realizaron todas las pruebas fisiológicas y bioquímicas correspondientes, así como la prueba de antagonismo in vitro contra los fitopatógenos Fusarium oxysporum, Colletotrichum gloeosporioides y Rhizoctonia solani. Cinco cepas produjeron una mayor cantidad de AIA en relación a las otras en presencia de triptófano, la cepa ES1 (Ochrobactrum sp.) produjo HCN, el 50 % de las cepas evaluadas liberaron sideróforos, el 60 % disolvió fósforo, y todas resultaron positivas para la fijación de nitrógeno. Nueve cepas inhibieron el crecimiento de F. oxysporum entre 40 % y 65 %, la cepa Alf (Pseudomonas fluorescens) inhibió además el crecimiento de C. gloeosporioides en un 22 %, y ninguna inhibió el crecimiento de R. solani. Los rizobios evaluados y la cepa de Pseudomonas fluorescens podrían ejercer efectos beneficiosos sobre las plantas a través de mecanismos directos e indirectos, o una combinación de ambos, lo que las convierte en una opción sostenible para la producción de cultivos.


ABSTRACT Rhizobacteria are part of the large number of microorganisms that act as biocontrol agents, producing metabolites that induce systemic resistance in plants and inhibit the growth of pathogens. The objective of this research was to evaluate the capacity of ten rhizobacteria of the genera Rhizobium, Bradyrhizobium, Sinorhizobium, Ochrobactrum and Pseudomonas to produce hydrogen cyanide (HCN), siderophores and indole acetic acid (IAA), dissolve phosphate, fix nitrogen and inhibit the growth of phytopathogens. All the corresponding physiological and biochemical tests were carried out, in addition to an in vitro antagonism test against the phytopathogens Fusarium oxysporum, Colletotrichum gloeosporioides and Rhizoctonia solani. Five strains produced a greater amount of IAA with respect to the others in the presence of tryptophan, the strain ES1 (Ochrobactrum sp.) produced HCN, 50% of the evaluated strains released siderophores, 60% solubilized phosphorus and all were positive for nitrogen fixation. Nine strains inhibited the growth of F. oxysporum by 40% to 65%. The Alf strain (Pseudomonas fluorescens) inhibited the growth of C. gloeosporioides by 22% while none inhibited the growth of R. solani. The rhizobia tested and the Pseudomonas fluorescens strain may have favorable effects on plants through direct and indirect mechanisms, or a combination of both, making them a sustainable option for crop production.

8.
Tempo psicanál ; 53(1): 30-57, jan.-jun. 2021.
Article in Portuguese | LILACS-Express | LILACS, INDEXPSI | ID: biblio-1341711

ABSTRACT

A neurose obsessiva é uma das duas psiconeuroses de defesa descritas por Freud e sobre as quais se baseou fundamentalmente a construção e desenvolvimento de seu modelo de aparelho psíquico. No presente artigo, nos propomos promover um estudo sobre a neurose obsessiva, sua dinâmica e mecanismos de defesa. Para tal, desenvolveremos uma revisão de literatura sobre o tema tomando como ponto de partida a consideração angular de Castel (2011), segundo a qual teria sido a neurose obsessiva e não a histeria a responsável pelo surgimento das bases da psicanálise e da construção da ideia de um aparelho psíquico. A fim de circunscrever a ideia proposta por Castel, nos dedicaremos prioritariamente a um percurso sobre a construção da neurose obsessiva pautados nas proposições freudianas. Seguiremos a linha temporal buscando lançar luz sobre o desenvolvimento de uma forma de organização própria a essa neurose e sua dinâmica do desejo em pauta. Por fim, apresentaremos as considerações de Abraham a partir das quais desenvolveremos a ideia de um movimento de regressão libidinal posta em questão nesses casos, e não uma lógica de fixação a um estágio específico do desenvolvimento libidinal.


Obsessive neurosis is one of the two defense psychoneuroses described by Freud and on which the construction and development of his model of psychic apparatus was fundamentally based. In this article, we propose to promote a study on obsessional neurosis, its dynamics and defense mechanisms. To this end, we will develop a literature review on the subject taking Castel's (2011) angular consideration as a starting point, according to which it was obsessive neurosis and not hysteria that was responsible for the emergence of the bases of psychoanalysis and the construction of the idea of a psychic apparatus. In order to circumscribe the idea proposed by Castel, we will dedicate ourselves primarily to a journey on the construction of obsessional neurosis based on Freudian propositions. We will follow the timeline seeking to shed light on the development of a form of organization specific to this neurosis and its dynamics of desire on the agenda. Finally, we will present Abraham's considerations from which we will develop the idea of a libidinal regression movement in question in these cases and not a logic of fixation to a specific stage of libidinal development.


La névrose obsessionnelle est l'une des deux psychonévroses de défense décrites par Freud et sur lesquelles reposait fondamentalement la construction et le développement de son modèle d'appareil psychique. Dans cet article, nous proposons de promouvoir une étude sur la névrose obsessionnelle, sa dynamique et ses mécanismes de défense. Pour cela, nous développerons une revue de la littérature sur le sujet en prenant comme point de départ la considération angulaire de Castel (2011), selon laquelle c'est la névrose obsessionnelle et non l'hystérie qui a été responsable de l'émergence des bases de la psychanalyse et de la construction de l'idée d'un appareil psychique. Afin de circonscrire l'idée proposée par Castel, nous nous consacrerons principalement à un voyage sur la construction de la névrose obsessionnelle à partir de propositions freudiennes. Nous suivrons la chronologie cherchant à éclairer le développement d'une forme d'organisation propre à cette névrose et sa dynamique du désir à l'ordre du jour. Enfin, nous présenterons les considérations d'Abraham à partir desquelles nous développerons l'idée d'un mouvement de régression libidinale en question dans ces cas et non une logique de fixation à un stade spécifique du développement libidinal.

9.
Rev. inf. cient ; 100(2): e3411, mar.-abr. 2021.
Article in Spanish | LILACS, CUMED | ID: biblio-1251822

ABSTRACT

RESUMEN Introducción: Múltiples reportes de la literatura científica avalan, que los trastornos del olfato y el gusto son cada vez más frecuentes desde el inicio de la COVID-19, ofreciendo diversos mecanismos fisiopatológicos para explicar estas alteraciones. Objetivo: Sistematizar las evidencias científicas disponibles, relacionadas con alteraciones del olfato y gusto producidas por SARS-CoV-2. Método: Se desarrolló una revisión bibliográfica basada en el análisis documental, la sistematización y contrastación de la información científica realizada sobre el tema. Las búsquedas se realizaron en bases de datos disponibles (SciELO, Medline, Scopus, Medscape), con el buscador Google Académico. Desarrollo: La información se estructuró en aspectos morfofuncionales de los sistemas del olfato y el gusto; estructura y replicación del SARS-CoV-2; interacción del virus con el sistema renina-angiotensina-aldosterona, su afinidad por el receptor enzima convertidora; mecanismos fisiopatológicos de estas disfunciones. Conclusiones: Aunque se describen las alteraciones del olfato y el gusto en pacientes con COVID-19, no están totalmente esclarecidos los mecanismos fisiopatogénicos relacionados con estas disfunciones quimiosensoriales. No obstante, su aparición en ausencia de otras enfermedades respiratorias, debe alertar al personal de salud sobre la posibilidad de infección por SARS-CoV-2.


ABSTRACT Introduction: Multiple scientific reports certified that disorders of taste and smell are more frequent since COVID-19 onset, reporting various pathophysiological mechanisms to explain these alterations. Objective: To methodize the available scientific evidence related to smell and taste disorders caused by SARS-CoV-2. Method: A bibliographic review, based on the documentary analyses, systematization and contrasting view of scientific information on the topic, was carried out. Search was conducted on available databases (SciELO, Medline, Scopus, Medscape), using the web search engine Academic Google. Development: Information gathered was performed based on taste and smell morphofunctional aspects; structure and replication of SARS-CoV-2; virus interaction with renin-angiotensin-aldosterone system and its affinity for the converting enzyme receptor; pathophysiological mechanisms of these dysfunctions. Conclusions: Although alterations of smell and taste are described in patients with COVID-19, the pathophysiological mechanisms associated with these chemosensory dysfunctions are not totally clarified. However, the onset of these symptoms in absence of other respiratory illnesses should alert health care personnel concerning to a possible SARS-CoV-2 infection.


RESUMO Introdução: Diversos relatos da literatura científica sustentam que os distúrbios do olfato e do paladar são cada vez mais frequentes desde o início da COVID-19, oferecendo vários mecanismos fisiopatológicos para explicar essas alterações. Objetivo: Sistematizar as evidências científicas disponíveis relacionadas às alterações de olfato e paladar produzidas pelo SARS-CoV-2. Método: Foi desenvolvida uma revisão bibliográfica a partir da análise documental, da sistematização e do contraste das informações científicas realizadas sobre o assunto. As buscas foram realizadas nas bases de dados disponíveis (SciELO, Medline, Scopus, Medscape), com a ferramenta de busca Google Scholar. Desenvolvimento: A informação foi estruturada em aspectos morfofuncionais dos sistemas olfativo e gustativo; estrutura e replicação de SARS-CoV-2; interação do vírus com o sistema renina-angiotensina-aldosterona, sua afinidade pelo receptor enzima conversora; mecanismos fisiopatológicos dessas disfunções. Conclusões: Embora as alterações do olfato e do paladar sejam descritas em pacientes com COVID-19, os mecanismos fisiopatogênicos relacionados a essas disfunções quimiossensoriais não estão totalmente esclarecidos. No entanto, seu aparecimento na ausência de outras doenças respiratórias deve alertar o pessoal de saúde para a possibilidade de infecção pelo SARS-CoV-2.


Subject(s)
Ageusia/physiopathology , SARS-CoV-2 , Anosmia/physiopathology , COVID-19/etiology , COVID-19/physiopathology
10.
Hematol., Transfus. Cell Ther. (Impr.) ; 43(1): 50-57, Jan.-Mar. 2021. tab, graf
Article in English | LILACS | ID: biblio-1154292

ABSTRACT

ABSTRACT Introduction: Chronic Myeloid Leukemia (CML) is a myeloproliferative disease that affects mainly adults between 50 and 55 years. In Brazil, information from the Sistema Único de Saúde (SUS) Outpatient Information System indicates that 12,531 patients had the Autorização de Procedimento Ambulatorial (APAC) approved for the CML treatment in 2017. Disease monitoring through molecular response evaluation is critical to the care of CML patients. The quantitative PCR test (real-time polymerase chain reaction) provides adequate evaluation parameters that allow the health professional to intervene at the right moments in order to reduce the chance of progression of the disease, providing the best outcome to the patient, including the possibility of treatment discontinuation for eligible patients. Although the test is included in the Clinical Protocol and Therapeutic Guidelines (PCDT) of CML, it is not possible to monitor the molecular response within SUS since there is no reimbursement for this test. Objective: Obtain expert recommendations on the importance, financing, and reimbursement of molecular monitoring in SUS. Methods: Six CML experts with different perspectives participated in the panel. The discussion was based in the main publications about the quantitative PCR test in CML monitoring. Results: Experts' recommendations: Molecular monitoring should be part of the integral treatment of patients with CML to reduce the chances of disease progression and costs to the health system; The government should put into practice what is provided in the PCDT of Chronic Myeloid Leukemia in Brazil: performing the monitoring of the molecular response via quantitative PCR; The government should create a code with adequate nomenclature and reimbursement value in SIGTAP, so that the test is carried out and covered by the public health network, as it is contained in the PCDT of the disease and the existing APAC does not cover the operational costs for its performance; Patients with chronic phase CML should perform a quantitative PCR every 3 months and, after reaching the MMR, should perform the examination every 6 months, as recommended by international guidelines; Patients should be monitored in reference laboratories that are standardized according to the international scale; The laboratories that are within the reference public centers could absorb all the test demand in Brazil, and other centers could be qualified through an ABHH accreditation; Adequate molecular monitoring may allow some patients to stop taking drugs and selffinancing the molecular test for all SUS patients Conclusion: A solution for the molecular test (BCR-ABL1) funding is urgent to ensure the monitoring of CML patients in SUS. The savings that might be generated with patients that stop taking the medication when adequately monitored may finance the test.


Subject(s)
Humans , Middle Aged , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/therapy , Real-Time Polymerase Chain Reaction , Reimbursement Mechanisms , Unified Health System , Brazil , Genes, abl
11.
Rev. bras. med. fam. comunidade ; 16(43): 2856, 20210126. tab
Article in Portuguese | LILACS | ID: biblio-1282025

ABSTRACT

Este artículo analiza el tema de la lista de pacientes como modelo para vincular a la población con los equipos de Atención Primaria de Salud (APS). Objetivo: destacar las características de la lista de pacientes como modelo de pago. Método: ensayo teórico sobre modelos de pago que analiza el piloto de lista de pacientes de la Secretaría Municipal de- Salud (SMS) de Florianópolis. Para fundamentar los principios de la lista de pacientes, su aplicación se presenta en dos contextos diferentes: (a) en el sistema de salud cerrado, como en las Organizaciones de Mantenimiento de la Salud (HMO) en los Estados Unidos; y (b) en el sistema de salud universal, utilizando el sistema de salud británico como modelo. Discusión: el proyecto piloto de lista de pacientes de la SMS de Florianópolis enfrenta dos problemas estructurales: (a) el sistema de salud universal que caracteriza a la APS brasileña; y (b) el modelo de pago de salarios. Estos dos componentes plantean el desafío de establecer un límite real para las listas de pacientes. Sin límite en el número de pacientes, no es posible ajustar la carga de trabajo de los profesionales de la salud. Adicionalmente, una lista de pacientes basada en el uso repetido y el atendimiento de casos de agudeza fuera de la lista tiende a incrementar la carga de trabajo de los profesionales con el tiempo. No obstante, la lista de pacientes propuesta proporciona una mayor visibilidad de la carga de trabajo facilitando tanto la gestión como el seguimiento de la presión asistencial y la redistribución interna de los pacientes entre los equipos de salud de la APS. También permite justificar la ampliación de los equipos de la Estrategia Salud de Familia (ESF) según datos más fiables sobre la realidad de los servicios e implementar programas de mejora de la calidad. Conclusión: la implementación de la modalidad de vinculación flexible en Florianópolis no tiene impactos positivos directos en la carga de trabajo de los equipos de salud, pero quizás sí de manera indirecta a través de la gestión inteligente de su sistema de APS.


This article discusses the theme of patient list as a model for registering the population to the Primary Health Care (PHC) teams. Objective: to highlight the characteristics of patient list as a payment model. Method: theoretical essay on payment models that analyses the pilot of patient list of the Municipal Health Secretariat (MHS) of Florianopolis. To support the principles of the patient list, its application is presented in two different contexts: (a) in closed health systems, as in the Health Maintenance Organizations (HMO) in the United States; and (b) in universal health systems, using the British health system as a model. Discussion: the Florianopolis MHS patient list pilot-project faces two structural problems: (a) the universal health system that characterises Brazilian PHC; and (b) the salary payment model. These two components pose the challenge of establishing a real cap for patient lists. Without a limit on the number of patients, it is not possible to adjust health professionals' workload. Additionally, a list of patients based on repeated use and on the care of out-of-list acute cases tend to increase the workload of professionals over time. Nonetheless, the proposed list of patients provides greater visibility of the workload facilitating both management and monitoring of care pressure and the internal redistribution of patients among the PHC health teams. It also makes it possible to justify the expansion of Family Health Strategy (FHS) teams according to more reliable data on the reality of services and to implement quality improvement programmes. Conclusion: the implementation of the flexible registering modality in Florianopolis does not have direct positive impacts on the workload of health teams, but perhaps it does indirectly through intelligent management of its PHC system.


Este artigo discute o tema da lista de pacientes como modelo de vinculação da população às equipes de Atenção Primária à Saúde (APS). Objetivo: evidenciar as características da lista de pacientes enquanto modelo de pagamento. Método: ensaio teórico sobre modelos de pagamento que analisa o piloto de lista de pacientes da Secretaria Municipal de Saúde (SMS) de Florianópolis-SC. Para fundamentar os princípios da lista de pacientes apresenta-se sua aplicação em dois contextos diferentes: (a) em sistemas fechados de saúde, como nas Health Maintenance Organizations (HMO) nos Estados Unidos; e (b) em sistemas universais de saúde, utilizando o sistema de saúde britânico como modelo. Discussão: o projeto piloto de lista de pacientes da SMS/Florianópolis enfrenta dois problemas estruturais: (a) o sistema de saúde universal que caracteriza a APS brasileira; e (b) o modelo de pagamento salarial. Esses dois componentes impõem o desafio de se estabelecer um teto real para as listas de pacientes. Sem um limite no número de pacientes não é possível adequar a carga de trabalho dos profissionais de saúde. Adicionalmente, uma lista de pacientes construída com base na utilização repetida e no atendimento de casos agudos não fidelizados tende a aumentar a carga de trabalho dos profissionais com o passar do tempo. Entretanto, a proposta da lista de pacientes confere maior visibilidade da carga de trabalho para a gestão, facilitando o monitoramento da pressão assistencial e a redistribuição interna dos usuários entre as equipes de saúde da APS. Permite também justificar a expansão das equipes de ESF de acordo com dados mais fidedignos da realidade dos serviços e implementar programas de melhoria de qualidade. Conclusão: a implantação da modalidade de vinculação flexível em Florianópolis não traz impactos positivos diretos na carga de trabalho das equipes, mas talvez de forma indireta por meio de uma gestão inteligente da rede da APS.


Subject(s)
Reimbursement Mechanisms , Workload , Family Health Strategy , Contracts , Family Practice
12.
Rev. bras. med. fam. comunidade ; 16(43): 2768, 20210126. tab, ilus
Article in Portuguese | LILACS | ID: biblio-1282463

ABSTRACT

Introdução: O atributo de APS "Orientação Comunitária" (OC) envolve um processo contínuo de atendimento às demandas de saúde de uma comunidade definida, utilizando-se de planejamento epidemiológico. Porém, sua definição tem se tornado bastante confusa pois qualquer serviço direcionado a uma comunidade específica termina por ser caracterizado como tal. Pouco parece se produzir sobre a OC no Brasil e no mundo. Objetivo: Realizar revisão sistematizada acerca do atributo Orientação Comunitária na literatura nacional e internacional. Metodologia: Trata-se de uma revisão integrativa, utilizando a estratégia PICo para selecionar publicações nas bases de dados PubMed, LILACS, Scielo e CAPES utilizando o descritor OC. Resultados: Foram encontrados 44 artigos e após exclusão de artigos repetidos e aplicação dos critérios de inclusão sobraram 9 artigos, 6 nacionais e 3 internacionais, 6 utilizando o Primary Care Assessment Tool (PCATool). Discussão: Todos os artigos trazem definições similares da OC, destacando a importância da epidemiologia e do contato com a comunidade para a definição de estratégias. Apesar da ESF ser na teoria um serviço orientado à comunidade, os 6 trabalhos nacionais que usaram o PCATool obtiveram baixos escores. Os Agentes Comunitários de Saúde (ACS) foram apenas mencionados em 3 artigos. A discussão sobre novas formas de comunidade não apareceu nesta revisão.


Scope The attribute of PHC "Community Orientation" (CO) requires a continuous process of meeting the health needs of a defined community using epidemiological planning. However, its definition has become quite confusing since any community-oriented service ends up being classified as CO. Besides, not enough research has been done about it in Brazil nor the world. Purposes To conduct a systematic review about the attribute Community Orientation in both national and international literature. Methodology: It is an integrative review in which PICo strategy was used in order to find publications in databases such as PubMed, LILACS, Scielo and CAPES using the descriptor CO. Results 44 articles were found and after excluding repeated ones and applying the inclusion criteria, 9 articles remained, of which 6 national and 3 international ones, 6 using the Primary Care Assessment Tool (PCATool). Theoretical Approach: All articles have similar definitions about CO, highlighting the importance of epidemiology and involvement with the community to better define health strategies. Although, in theory, Brazilian Family Health Strategy (FHS) is a PHC community-oriented service, all 6 Brazilian publications that used PCATool reached low scores. The Community Health Workers (CHW) were only mentioned in 3 articles. The discussion about new shapes of communities did not appear in this review.


Introducción: El atributo de la APS "Orientación Comunitaria" (CO) implica un proceso continuo de satisfacción de las demandas de salud de una comunidad definida, utilizando la planificación epidemiológica. Sin embargo, su definición se ha vuelto bastante confusa ya que cualquier servicio dirigido a una comunidad específica acaba siendo caracterizado como tal. Poco parece producirse sobre OC en Brasil y en el mundo. Objetivo: Realizar una revisión sistemática del atributo Orientación comunitaria en la literatura nacional e internacional. Metodología: Se trata de una revisión integradora, utilizando la estrategia PICo para seleccionar publicaciones en las bases de datos PubMed, LILACS, Scielo y CAPES utilizando el descriptor OC. Resultados: se encontraron 44 artículos y luego de excluir los artículos repetidos y aplicar los criterios de inclusión, quedaron 9 artículos, 6 nacionales y 3 internacionales, 6 utilizando la Herramienta de Evaluación de Atención Primaria (PCATool). Discusión: Todos los artículos tienen definiciones similares de CO, destacando la importancia de la epidemiología y el contacto con la comunidad para la definición de estrategias. Si bien la ESF es en teoría un servicio orientado a la comunidad, los 6 estudios nacionales que utilizaron el PCATool obtuvieron puntuaciones bajas. Los agentes comunitarios de salud (CHA) solo se mencionaron en 3 artículos. La discusión de nuevas formas de comunidad no apareció en esta revisión.


Subject(s)
Primary Health Care , Health Care Evaluation Mechanisms , Community Health Workers , Universal Access to Health Care Services , Family Practice
13.
Article in Chinese | WPRIM | ID: wpr-909227

ABSTRACT

In recent years, programmed death protein 1 and its ligand (PD-1/PD-L1) pathway as the main research direction of tumor therapy has brought changes to the treatment of non-small cell lung cancer in some patients. The curative effects of anti-PD-L1 inhibitors are positively correlated with the expression of PD-L1 in tumor tissues. However, different techniques are required to detect the expression of PD-L1 after application of different PD-1/PD-L1 inhibitors. Therefore, how to detect the expression of PD-L1 in tumor tissues efficiently and accurately is particularly important. This review summarizes the clinical significance and detection methods of PD-L1 expression in non-small cell lung cancer.

14.
Article in Chinese | WPRIM | ID: wpr-908732

ABSTRACT

Objective:To explore the protective effect of ulinastatin on sepsis-related acute lung injury (ALI) in mice and its related mechanism analysis.Methods:A total of 50 male mice aged 6-8 weeks were selected and randomly divided into 5 groups, with 10 mice in each group. Group A was the sham operation group, group B was the sham operation and ulinastatin (30 000 U/kg) intervention group, group C was the simple cecal ligation and perforation (CLP) group, group D was the CLP+low-dose ulinastatin (15 000 U/kg) treatment group, and group E was the CLP+high-dose ulinastatin (30 000 U/kg) treatment group. Ulinastatin in groups B, D and E was injected intraperitoneally at the corresponding dose 1 h before CLP operation. The hematological characteristics, lung edema and inflammatory changes of lung tissue in mice of each group were evaluated and the expression levels of interleukin(IL)-6, tumor necrosis factor(TNF)-ɑ, IL-1β and nuclear factor(NF)-κB p65 mRNA and protein expression in blood and lung tissue were compared among the five groups.Results:Compared with that in group A, the lung water content in group C was significantly increased: (78.68 ± 1.85)% vs. (51.98 ± 0.77)%, P<0.01. Compared with that in group C, pulmonary edema of the mice treated with ulinastatin was significantly reduced, with a certain dose-effect relationship. The wet weight/dry weight ratio of the lung tissue in group C was significantly higher than that of group A, B, D, E: 4.74 ± 0.28 vs. 2.23 ± 0.16, 2.25 ± 0.22, 2.89 ± 0.31, 2.09 ± 0.12, P<0.01, and group E had the lowest ratio. After ulinastatin intervention, the inflammatory manifestations of the lungs were reversed in a dose-dependent manner. The degree of structural destruction was improved, and edema and polymorphonuclear neutrophil infiltration were reduced. Ulinastatin could significantly reduce the neutrophil and lymphocyte counts of mice after CLP treatment, reduce the expression of IL-6, TNF-α, IL-1β inflammatory cytokines and MPO activity. The reverse transcription-polymerase chain reaction (RT-PCR) results and Western blot analysis showed that the mRNA and protein expressions of NF-κB p65 in group C of lung tissue was significantly increased. However, after treatment with ulinastatin, it was significantly reduced, and it had a certain dose-effect relationship. Conclusions:Ulinastatin pretreatment can significantly reduce the expression levels of IL-6, IL-1β and TNF-α in sepsis-related acute lung injury, and can alleviate the inflammatory response in the lung. The protective effect of ulinastatin may be related to the inhibition of NF-κB pathway activation.

15.
Article in Chinese | WPRIM | ID: wpr-908693

ABSTRACT

Objective:To explore the molecular mechanism of carbapenem antibiotic resistance in burns patients infected with Klebsiella pneumoniae.Methods:The patients who were diagnosed with Klebsiella pneumoniae in the burns department of Taizhou Central Hospital (Affiliated Hospital of Taizhou University) from May 2018 to October 2019 were enrolled.Ten carbapenem-resistant Klebsiella pneumoniae (CR-KP) bacterium were isolated, then the degree of resistance of CR-KP bacteria was analyzed.The common resistance genes was detected by polymerase chain reaction (PCR), the existence of resistance genes was verified through plasmid conjugation tests, and the expression of OmpK36 protein in the membrane pores was analyzed.Results:The 10 strains in this study all showed different degrees of drug resistance. The results of PCR showed that all drug resistant bacteria carried the NDM gene, and 4 strains carried the KPC gene. In addition, only a small number of resistant bacteria carried extended-spectrum β-lactamase (ESBLs) genes and AmpC genes. Plasmid conjugation experiments showed that most of the Zygomycetes were resistant to carbapenem antibiotics, and the Western blot results indicated that the protein expression of OMPK36 in the membrane pore of Zygomycetes was decreased.Conclusions:The resistance of CR-KP bacteria in burns patients was mainly caused by plasmid-mediated NDM gene insertion and reduced expression of OMPK36 protein in the membrane pore.

16.
Article in Chinese | WPRIM | ID: wpr-907702

ABSTRACT

Objective:By using the network pharmacology method to predict the active constituents and action targets of Suzi-Jiangqi Decoction in the treatment of COPD, and to explore its potential molecular mechanism with multi-component, multi-target and multi-pathway characteristics. Methods:The active constituents and targets of Suzi-Jiangqi Decoction were collected, screened and predicted according to the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and UniProt software. Search for the gene targets related to COPD in GeneCards, online human Mendelian genetic database (OMIM) and TTD database. The intersection targets of component targets and disease targets were obtained by Veen map online software. The network model with the sequence of active constituents-target-disease was constructed and analyzed by Cytoscape software, and the protein-protein interaction network (PPI) was constructed by STRING database. The gene ontology function annotation (GO) and Tokyo genome encyclopedia (KEGG) pathway enrichment analysis of common targets with metascape online tool. Results:A total of 163 active constituents of Suzi-Jiangqi Decoction were screened, 283 targets were predicted, and 159 targets involved in the treatment of COPD. Quercetin, kaempferol, naringin and luteolin were the key active ingredients. IL6, TNF, MAPK3, JUN, CASP3, CXCL8, CXCL10, MMP9 and MAPK1 were important gene targets. GO analysis showed that the biological processes involved in the enrichment of key gene targets included the response to bacteria, the cytokine mediated signaling pathway, the cell's response to inorganic substances, the response to oxidative stress, the response to LPS, and so on. The enrichment analysis of KEGG pathway showed that the signaling pathway of Suzi-Jiangqi Decoction in the treatment of COPD included TNF signaling pathway, IL-17 signaling pathway, cell cycle, Influenza A, HTLV-I infection, AGE-RAGE signaling pathway, Tuberculosis, Epstein-Barr virus infection and so on. Conclusion:Suzi-Jiangqi Decoction can treat COPD through multi-target and multi-pathway mechanisms of anti-inflammatory, anti infection and immune regulation, which lays a foundation for further study of its molecular mechanism.

17.
Article in Chinese | WPRIM | ID: wpr-907684

ABSTRACT

Objective:To explore the effective chemical constituents and target genes of the Sanhan-Qushi-Wenjing-Tongluo formula through the method of network pharmacology, and to further analyze the mechanism of treatoffing psoas fasciitis. Methods:The TCMSP database was used to search and screen the chemical active substances of Sanhan-Qushi-Wenjing-Tongluo formula and its target proteins acting on the human body. At the same time, the GeneCards database platform was used to predict the target of disease and the active ingredient-target network was constructed. Construct a PPI network through the STRING database, search for PPI core genes, and then perform GO enrichment analysis and KEGG enrichment analysis to find the signal pathways involved and construct a target-path network. Results:Through screening, a total of 23 key chemical components and 25 common target proteins was obtained in Sanhan-Qushi-Wenjing-Tongluo formula treating psoas fasciitis; gene analysis of enrichment analysis results include antibiotic response, cyclin-dependent proteins kinase holoenzyme complex, cytokine receptor binding, etc. Kyoto Encyclopedia of Genes and Genomes enrichment analysis results include AGE-RAGE signaling pathway, measles, endocrine resistance, inflammatory bowel disease, etc; the target genes gained which have a higher degree of matching with the above mentioned pathways include IL6, JUN, IL1B, CDK4, CCND1. Conclusion:Sanhan-Qushi-Wenjing-Tongluo formula could treat psoas fasciitis by regulating the target genes such as IL6, JUN, IL1B, CDK4 and CCND1.

18.
Article in Chinese | WPRIM | ID: wpr-907683

ABSTRACT

Objective:To analize the mechanism of Xiaoxianxiong Decoction on myocardial ischemiareperfusion injury by network pharmacological method and molecular docking technology. Methods:The effective components and corresponding target proteins of Xiaoxianxiong Decoction were screened by TCMSP, and the predicted target protein names were converted to gene names in UniProt database. The gene target of myocardial ischemia reperfusion injury was screened through the GeneCards and OMIM database. Venn online software was used to obtain the common target of drugs and diseases, then the visual analysis and the "compound-target" network diagram was constructed by using Cytoscape software. The protein interaction network was made by using STRING database and Cytoscape software, and the network topology was analyzed. Molecular docking software (autodock Vina) was used to verify the molecular docking between the top five active components and the top ten core targets, and the GO function of target genes and enrichment analysis of KEGG pathway were analyzed by Bioconductor R software package. Results:After the screening, 38 effective chemical components and 187 target genes of Xiaoxianxiong Decoction and 511 disease-related target genes were obtained. 72 common target genes of drug diseases were obtained. The core targets involved AKT1, MMP9, IL1B, EGF, etc. Go function analysis showed 1 095 biological processes, 24 cell components, 61 molecular functions, and KEGG pathway analysis found 111 related signal pathways. Conclusion:This study predicted that Xiaoxianxiong Decoction could treat myocardial ischemia-reperfusion injury through multiple targets such as AKT1, MMP9, IL1B, EGF, and multiple pathways such as IL-17 signaling pathway and PI3K-Akt signaling pathway, which laid a foundation for further study on the material basis and molecular mechanism of this compound.

19.
Article in Chinese | WPRIM | ID: wpr-907667

ABSTRACT

Objective:Network pharmacology was used to investigate the mechanism of the Sargassum treating thyroid nodule. Methods:The main active ingredients of Sargassum and the targets of active ingredients were obtained by TCMSP, and thyroid nodule disease targets were obtained through GeneCards and OMIM. The STRING database and Cytoscape 3.2.1 software were used to construct the active ingredients-disease-targets network and protein interaction network (PPI). The GO enrichment and KEGG pathway analysis of the targets were analyzed by using R version 4.0.0 software. Results:Two active ingredients were screened from Sargassum and 59 targets were related to thyroid nodule. Biological function analysis showed that the targets of Sargassum included DNA-binding transcription activator activity, RNA polymerase Ⅱ-specific, ubiquitin-like protein ligase binding. Sargassum played an important role in treating thyroid nodule by the gene targets such as RELA, CASP3, IL6, CASP9, EGFR, VEGFA and other targets mediating the signaling pathways such as PI3K-Akt signaling pathway, Kaposi sarcoma-associated herpesvirus infection and other pathways. Conclusion:The potential multi-target and multi-pathway network mechanism of Sargassum in the treatment of thyroid nodule provided theoretical basis for further research.

20.
Article in Chinese | WPRIM | ID: wpr-907666

ABSTRACT

Objective:To explore the targets and signal pathways of Maxing-Ersan Decoction in the treatment of COPD by using network pharmacology, and to reveal the mechanism of intervention of multiple targets and multiple pathways of Maxing-Ersan Decoction in the treatment of COPD. Methods:The active ingredients and corresponding targets of Maxing-Ersan Decoction were screened with the help of TCMSP database, and the targets related to COPD disease were screened with GeneCards database. The STRING database was used for protein-protein interaction (PPI) network analysis. By using Cytoscape to build compound target network and PPI network; by using Draw Venn Diagram website to draw a Venn diagram, and using R software to perform gene ontology (GO) functional enrichment analysis and Kyoto encyclopedia of genes and genomes (KEGG). Results:A total of 101 active ingredients were obtained, with 250 corresponding targets, including 214 targets related to COPD. Through GO and KEGG enrichment analysis, all together 48 signal pathways related to the main components of Maxing-Ersan Decoction were screened out. Conclusion:Based on the network pharmacological analysis, the effective chemical components of Maxing-Ersan Decoction were found, and it was found that Maxing-Ersan Decoction may act on COPD through multiple targets and multiple pathways such as inflammation and immune regulation.

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