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1.
Rev. cuba. estomatol ; 58(2): e3162, 2021. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1289399

ABSTRACT

Introducción: Las enfermedades de la cavidad bucal en los pacientes con VIH/sida pueden verse agravadas dependiendo de la respuesta inmunitaria del paciente y los niveles de linfocitos. Objetivo: Relacionar los niveles de linfocitos T CD4 y las principales lesiones bucales en pacientes con el VIH/sida del Hospital Nacional Hipólito Unanue (Lima, Perú), durante el 2018. Métodos: Se realizó un estudio analítico y de corte transversal, entre julio y octubre del 2018, en 65 pacientes hospitalizados, a los cuales se realizó un examen clínico de la cavidad bucal. Se evaluó la presencia de manifestaciones bucales asociadas al VIH/sida; también se clasificó el nivel de linfocitos T CD4 en tres categorías (> 500 cel/mm3, entre 200-500 cel/mm3 y < 200 cel/mm3). Resultados: Un 70,8 por ciento de los pacientes no se encontraba con tratamiento antirretroviral al momento del examen. El nivel promedio de linfocitos T CD4 fue 237,65 cel/mm3, con mayor prevalencia en mujeres. El 56,9 por ciento de los pacientes presentaron lesiones bucales, el sexo masculino fue el más afectado (91 por ciento). La lesión más frecuente fue la candidiasis bucal (44,6 por ciento) y la categoría que presentó mayor frecuencia de lesiones bucales fue la < 200 cel/mm3 (38,5 por ciento; p < 0,05). Conclusiones: El sexo masculino presentó la mayor cantidad de lesiones bucales asociadas a bajos niveles de linfocitos T CD4. La mayor parte de lesiones bucales se presentaron en un nivel de linfocitos T CD4 < 200 cel/mm3. La candidiasis bucal fue la lesión que más se evidenció al momento de realizar el examen clínico(AU)


Introduction: Oral diseases may be aggravated in HIV/AIDS patients depending on their immune response and lymphocyte levels. Objective: Describe the relationship between CD4 T lymphocyte levels and the main oral lesions in HIV/AIDS patients from Hipólito Unanue National Hospital in Lima, Peru, during the year 2018. Methods: An analytical cross-sectional study was conducted of 65 hospitalized patients from July to October 2018. The patients underwent oral clinical examination. Evaluation was performed of the presence of HIV/AIDS-related oral manifestations, and CD4 T lymphocyte levels were classified into three categories: > 500 cell/mm3, 200-500 cell/mm3 and < 200 cell/lmm3. Results: Of the total patients studied, 70.8 percent were not under antiretroviral treatment at the moment of the examination. Average CD4 T lymphocyte level was 237.65 cell/mm3, with higher results among women. 56.9 percent of the patients had oral lesions. Males were more commonly affected (91 percent). The most frequent lesion type was oral candidiasis (44.6 percent), whereas the category presenting the highest frequency of oral lesions was < 200 cell/mm3 (38.5 percent; p < 0.05). Conclusions: Male patients presented the largest number of oral lesions associated to low CD4 T lymphocyte levels. Most of the oral lesions were found at a CD4 T lymphocyte level < 200 cell/mm3. Oral candidiasis was the lesion most commonly found by the clinical examination(AU)


Subject(s)
Humans , Male , Female , Candidiasis, Oral/etiology , T-Lymphocytes , Acquired Immunodeficiency Syndrome/epidemiology , Mouth/injuries , Cross-Sectional Studies
2.
Odontol. sanmarquina (Impr.) ; 24(1): 85-88, Ene-Mar. 2021.
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1150874

ABSTRACT

El objetivo de esta revisión es presentar el estado del conocimiento respecto a la capacidad que tiene Candida albicans de producir nitrosaminas y de cómo estas se relacionan con la posible malignización de lesiones de la cavidad bucal. La candidiasis oral (CO) es una infección frecuente de la mucosa bucal provocada por hongos de la familia Candida spp. Estos hongos son integrantes de la flora bucal habitual, pero, por diferentes factores locales y sistémicos, pueden producir micosis superficiales. Entre las distintas especies, albicans, es la más frecuente en la cavidad bucal.Posee gran variedad de factores de virulencia, uno de ellos la capacidad de nitrosación de nitratos y nitritos presentes en saliva que últimamente se ha considerado relacionada a la malignización de lesiones mucosas de la cavidad bucal y otras como el estómago.


This literature review was carried out to approach the state of knowledge regarding the capacity of Candida Albicans to produce nitrosamines and how these are related to the possible malignancy of lesions in the oral cavity. Oral candidiasis (CO) is a frequent infection of the oral mucosa caused by fungi of the family Candida spp. These fungi are part of the usual oral flora but, due to different local and systemic factors, can produce superficial mycosis. Among the different species, Albicans is the most frequent in the oral cavity. It has a great variety of virulence factors, one of them the nitrosation capacity of nitrates and nitrites present in saliva that lately has been considered related to the malignization of mucosal lesions of the oral cavity and others such as the stomach.

3.
Rev. epidemiol. controle infecç ; 10(3): 1-16, jul.-set. 2020. ilus
Article in English | LILACS | ID: biblio-1247638

ABSTRACT

Justificativa e Objetivos: A candidíase oral tem uma ocorrência comum em pacientes imunocomprometidos. No entanto, outras infecções emergentes tornaram-se cada vez mais habituais. O objetivo deste estudo foi investigar a prevalência, os determinantes de virulência e a suscetibilidade a antifúngicos de leveduras que colonizam a mucosa de pacientes imunocomprometidos na região Nordeste do Brasil. Métodos: A amostra foi composta por 60 pacientes HIV positivos atendidos no Serviço de Atendimento Especializado/Hospital Dia do Hospital Universitário Prof. Alberto Antunes, vinculado à Universidade Federal de Alagoas. As amostras foram coletadas em regiões subgengivais e semeadas em CHROMagar para confirmação presuntiva de Candida spp., seguido por PCR e sequenciamento. Além disso, testamos os determinantes de virulência fosfolipase e protease e avaliamos in vitro a concentração inibitória mínima dos antifúngicos anfotericina B e fluconazol. Este projeto foi aprovado pelo Comitê de ética em pesquisa do Centro de Estudos Superiores de Maceió. Resultados: Aproximadamente 63% dos pacientes foram colonizados por leveduras. A espécie C. albicans foi predominante, enquanto as espécies de Candida não-albicans representaram 49% dos isolados, sendo C. dubliniensis e C. parapsilosis as mais comuns. Entretanto, C. intermedia, Bullera penniseticola e Naganishia liquefaciens também foram encontrados. Os determinantes da virulência protease e/ou fosfolipase também foram produzidos por Candida spp. e alguns isolados oportunistas incomuns como Kodamaea ohmeri, N. liquefaciens e Saitozyma podzolica. Além disso, a maioria dos isolados de Candida spp. e algumas espécies oportunistas incomuns apresentaram altos valores de concentração inibitória mínima. Conclusão: Os resultados obtidos indicam que C. albicans continua a ser a espécie predominante na cavidade oral de pacientes imunodeficientes e, juntamente com outras espécies incomuns, pode apresentar alta resistência aos antifúngicos testados.(AU)


Background and Objectives: Oral candidiasis has a common occurrence in immunocompromised patients. However, other emergent infections have become increasingly common. The aim of this study was to investigate the prevalence, virulence determinants and the antifungal susceptibility of yeast colonizing the mucosa of immunocompromised patients in Northeastern Brazil. Methods: Samples from sixty HIV-positive patients seen at the Specialized Service / Hospital Dia - Hospital Universitário Prof. Alberto Antunes from the Federal University of Alagoas were collected from subgingival sites and seeded on CHROMagar for presumptive confirmation of Candida spp. followed by PCR and sequencing. In addition, we tested virulence determinants, phospholipase and protease and evaluated in vitro the Minimum Inhibitory Concentration of antifungals amphotericin B and fluconazole. This project was approved by the Research Ethics Committee of the Center for Higher Studies in Maceió. Results: Approximately 63% of the patients were colonized by yeasts, with C. albicans as the predominant species, while non-Candida albicans species accounted for 49% of the isolates, with C. dubliniensis and C. parapsilosis being the commonest, but C. intermedia, Bullera penniseticola and Naganishia liquefaciens were also found. The virulence determinants protease and/or phospholipase were also produced by Candida spp. and some uncommon opportunistic isolates such as Kodamaea ohmeri, N. liquefaciens and Saitozyma podzolica. Furthermore, most of Candida spp. strains and some uncommon opportunistic species showed high values of minimal inhibitory concentration. Conclusion: Results obtained indicate that C. albicans continues to be the predominant species in oral cavity of immunodeficient patients and along with other unusual species may present high resistance to the antifungals tested.(AU)


Justificación y Objetivos: La candidiasis oral acomete con frecuencia a pacientes inmunocomprometidos. Sin embargo, otras infecciones emergentes se han vuelto cada vez más comunes. El objetivo de este estudio fue investigar la prevalencia, la producción de determinantes de virulencia y la susceptibilidad a antifúngicos de levaduras que colonizan la mucosa de pacientes inmunocomprometidos en la región Nordeste de Brasil. Métodos: Se colectaron muestras de sesenta pacientes VIH positivos atendidos en el Servicio de Atención Especializado/Hospital Día del Hospital Universitario Prof. Alberto Antunes, vinculado a la Universidad Federal de Alagoas. Se colectaron las muestras en las regiones subgingivales y las sembraron en CHROMagar para la presunta confirmación de Candida spp. seguido de PCR y secuenciación. Además, analizamos los determinantes de virulencia fosfolipasa y proteasa y evaluamos in vitro la concentración mínima inhibitoria de los antifúngicos anfotericina B y fluconazol. Este proyecto fue aprobado por el Comité de Ética en Investigación del Centro de Estudios Superiores de Maceió. Resultados: Aproximadamente el 63% de los pacientes fueron colonizados por levaduras, y la C. albicans fue la especie predominante, mientras que las especies de Candida no-albicans representaron el 49% de los aislamientos, de las cuales la C. dubliniensis y la C. parapsilosis fueron las más comunes. Sin embargo, también se encontraron C. intermedia, Bullera penniseticola y Naganishia liquefaciens. Los determinantes de virulencia de proteasa y/o fosfolipasa también fueron producidos por Candida spp. y algunos aislados oportunistas inusuales como Kodamaea ohmeri, N. liquefaciens y Saitozyma podzolica. Además, la mayoría de los asilados de Candida spp. y algunas especies oportunistas inusuales mostraron valores altos de concentración mínima inhibitoria. Conclusión: Los resultados obtenidos indican que C. albicans continúa siendo la especie predominante en la cavidad oral de pacientes inmunodeprimidos y, junto con otras especies poco comunes, puede presentar una alta resistencia a los antifúngicos evaluados.(AU)


Subject(s)
Humans , Virulence , Yeasts/virology , Candida , Candidiasis, Oral , Virulence Factors , Immunologic Deficiency Syndromes , Antifungal Agents , Prevalence , Acquired Immunodeficiency Syndrome
4.
J Cancer Res Ther ; 2020 Jul; 16(3): 470-477
Article | IMSEAR | ID: sea-213843

ABSTRACT

Objective: The aim of the study is to assess the effect of probiotic bacteria on oral Candida counts in cancer patients who are undergoing head- and neck-radiotherapy in a tertiary care center. Study Design: The study was a randomized clinical trial including 90 patients who just completed head- and neck-radiotherapy. Materials and Methods: Participants were randomly allocated into three equal sized groups, i.e., probiotics group, candid group, and combination groups. Oral rinse samples of the patients were collected before and after the intervention for the identification of Candida. The samples were incubated on Sabouraud's Dextrose Agar with Chloramphenicol at 37°C for 48 h, to assess the counts of colony-forming units/milliliter (CFU/ml) of Candida in saliva, and further on chrome agar plates to identify the Candida spp. Data were analyzed using mixed ANOVA to compare mean CFU/ml of Candida among three groups before and after the intervention. Results: A total of 86 patients were included in the final analysis and there was a statistically significant reduction in mean Candida spp. Counts (CFU/ml) after intervention in all the three groups (P = 0.000) and significant reductions identified in both probiotic and combination therapy groups. Apart from reduction in Candida albicans, significant decrease in Candida glabrata and Candida tropicalis was observed after probiotics usage compared to other groups. Conclusions: The present study suggests that probiotic bacteria were effective in reducing oral Candida spp which can be recommended alone or in combination with traditional antifungal agents for effective reduction in oral Candida in head- and neck-radiotherapy patients

5.
Rev. cuba. estomatol ; 57(1): e1965, ene.-mar. 2020. tab, graf
Article in Spanish | LILACS, CUMED | ID: biblio-1126482

ABSTRACT

RESUMEN Introducción: Algunos tipos de cáncer suelen ser tratados con fármacos quimioterapéuticos que afectan de forma indirecta la cavidad bucal, los cuales susceptibilizan al paciente a contraer infecciones bucales como candidiasis bucal. Se ha evidenciado que una condición oncológica facilita una infección bucal por Candida albicans, sin embargo, otras especies de Candida se les relaciona poco. Objetivo: Identificar especies de Candida spp. en cavidad bucal de pacientes sometidos a quimioterapia. Métodos: Estudio descriptivo de corte transversal. Se tomaron 60 muestras de igual número de pacientes por conveniencia de un Instituto Oncológico en Barranquilla, Colombia. Previo a un examen clínico de diagnóstico de candidiasis bucal. Se tomaron hisopados en las superficies infectadas para realizar un examen directo -tinción de Gram y cultivo en agar dextrosa Sabouraud con cloranfenicol incubadas a 37 ºC durante 48 h y posteriormente cultivadas en medio CHROMagar® Candida- para identificación de diferentes especies. Se aplicó estadística descriptiva y de correlación. Resultados. El 55 por ciento de los pacientes correspondió al sexo femenino, con edades mayores a 60 años. El cáncer de mama fue el más frecuente en el 30 por ciento. La quimioterapia aplicada a todos fue el 5-fluorouracilo combinado con ciclofosfamida. El tipo clínico de candidiasis atrófica en lengua fue más frecuente en el 37 por ciento, seguida de candidiasis pseudomembranosa en lengua en el 33 por ciento. El 90 por ciento de muestras presentó algún tipo de Candida, crecimiento de una única especie y cultivos mixtos con más de una especie de Candida. Se evidenció el 13,3 por ciento de C. albicans y otro 13,3 por ciento de Candida spp. no albicans. Ninguno de los diagnósticos oncológicos tuvo una asociación estadísticamente significativa con las especies de Candida. Conclusiones: Se evidencia que la especie de C. albicans en candidiasis bucal en pacientes con cáncer, fue la más frecuente, con predominio en mayor proporción cultivos mixtos de Candida no albicans (C. glabrata, C. tropicalis) poco relacionados con este tipo de enfermedad(AU)


ABSTRACT Introduction: Some types of cancer are often treated with chemotherapeutic drugs which indirectly affect the oral cavity, thus making the patient vulnerable to infections such as oral candidiasis. Oral infection by Candida albicans has been found to be fostered by oncological conditions, but other Candida species have not been sufficiently analyzed in such a context. Objective: Identify Candida spp. in the oral cavity of patients undergoing cancer chemotherapy. Methods: A descriptive cross-sectional study was conducted of 60 samples of an equal number of patients selected by convenience sampling at an Oncological Institute in Barranquilla, Colombia, following diagnostic clinical examination for oral candidiasis. Swab samples were taken from the infected surfaces to perform direct examination - Gram staining and Sabouraud dextrose agar culture with chloramphenicol incubated at 37ºC for 48 h and then cultured in CHROMagar® Candida medium - for identification of various species. Analysis was based on correlation and descriptive statistics. Results: Of the total patients evaluated 55 percent were female and aged over 60 years. Breast cancer prevailed with 30%. The chemotherapy applied in all cases was 5-fluorouracil combined with cyclophosphamide. Atrophic candidiasis of the tongue was the most common clinical type with 37 percent, followed by pseudomembranous candidiasis of the tongue with 33 percent. Of the samples examined, 90 percent contained some sort of Candida, growth of a single species and mixed cultures with more than one Candida species. C. albicans represented 13.3 percent and non-albicans spp. another 13.3 percent. None of the oncological diagnostic analyses had a statistically significant association to Candida species. Conclusions: The species C. albicans was the most commonly found in oral candidiasis among cancer patients, with a predominance of non-albicans Candida spp. (C. glabrata, C. tropicalis) mixed cultures, a fact not generally related to this condition(AU)


Subject(s)
Humans , Candida albicans , Candidiasis, Oral/diagnosis , Neoplasms/drug therapy , Epidemiology, Descriptive , Cross-Sectional Studies , Observational Studies as Topic
6.
Rev. Pesqui. (Univ. Fed. Estado Rio J., Online) ; 12: 505-509, jan.-dez. 2020. tab
Article in English, Portuguese | LILACS, BDENF | ID: biblio-1097201

ABSTRACT

Objetivo: avaliar a eficácia antimicrobiana da terapia fotodinâmica no tratamento da candidíase oral em pessoas vivendo com HIV/aids. Método: estudo experimental, qualitativo e descritivo com 18 pessoas vivendo com HIV/aids que manifestavam a candidíase oral, maiores de 18 anos, que estavam em tratamento no Hospital Universitário Gaffrée e Guinle. Este grupo subdividiu-se em um grupo controle, composto por sete pessoas, que recebeu tratamento com a terapia fotodinâmica e antifúngicos, e um grupo experimental, com 11, que recebeu apenas a terapia fotodinâmica. A evolução do tratamento de cada participante foi acompanhada por registros fotográficos em duas consultas, inicial e final. Esta pesquisa foi aprovada pelo Comitê de Ética em Pesquisa do hospital, parecer número 2.431.107. Resultados: a maioria dos participantes apresentou melhora clínica, ainda que discreta, e em apenas um houve piora clínica. Conclusão: a terapia fotodinâmica antimicrobiana pode ser eficaz no tratamento da candidíase oral em pessoas vivendo com HIV/Aids


Objective: evaluate the antimicrobial efficacy of photodynamic therapy in the treatment of oral candidiasis in people living with HIV/ aids. Method: experimental, qualitative and descriptive study with 18 people living with HIV/aids who presented oral candidiasis, over 18 years of age, who were being treated at the Gaffrée and Guinle University Hospital. This group was subdivided into a control group, composed of seven people, who received treatment with photodynamic and antifungal therapy, and an experimental group, with 11, who received only the photodynamic therapy. The evolution of the treatment of each participant was followed by photographic registers in two appointments, initial and final. This research was approved by the Research Ethics Committee from the hospital, dictum number 2.431.107. Results: most of the participants showed clinical improvement, albeit discrete, and in only one there was clinical worsening. Conclusion: antimicrobial photodynamic therapy may be effective in the treatment of oral candidiasis in people living with HIV/Aids


Objetivo: evaluar la eficacia antimicrobiana de la terapia fotodinámica en el tratamiento de la candidiasis bucal en personas que viven con VIH/sida. Método: estudio experimental, cualitativo y descriptivo con 18 personas viviendo con VIH/sida que manifestaban la candidiasis bucal, mayores de 18 años, que estaban en tratamiento en el Hospital Universitario Gaffrée y Guinle. Este grupo se subdividió en grupo control, compuesto por siete personas, que recibió tratamiento con la terapia fotodinámica y antifúngicos, y un grupo experimental, con 11, que recibió sólo la terapia fotodinámica. La evolución del tratamiento de cada participante fue acompañada por registros fotográficos en dos consultas, inicial y final. La investigación fue aprobada por el Comité de Ética en Investigación del lugar, dictamen número 2.431.107. Resultados: la mayoría de los participantes presentó mejoría clínica, aunque discreta, y en apenas uno hubo empeoramiento clínico. Conclusión: la terapia fotodinámica antimicrobiana puede ser eficaz en el tratamiento de la candidiasis bucal en personas que viven con el VIH/Sida


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Photochemotherapy/statistics & numerical data , Candidiasis, Oral/therapy , AIDS-Related Opportunistic Infections/therapy , Photochemotherapy/methods , Candidiasis, Oral/epidemiology , Acquired Immunodeficiency Syndrome/therapy , HIV , Drug Resistance, Fungal
7.
Braz. J. Pharm. Sci. (Online) ; 56: e18456, 2020. tab, graf
Article in English | LILACS | ID: biblio-1249172

ABSTRACT

The aim of this study was evaluate the in vitro antifungal activity of crude extracts from Eugenia uniflora, Libidibia ferrea and Psidium guajava. The extracts were obtained by turbo-extraction using water (AQ) or acetone-water (AC-W) (7:3, v/v) as solvents and lyophilized to obtain the crude extracts (CE). The CE were characterized by UV-Vis, TLC and HPLC. The activity of CEs was investigated against clinical isolates of Candida spp. and the Minimum Inhibitory Concentration (MIC), MIC50 and MIC90 were determinated. The analysis by TLC showed that all CEs presented polyphenols (flavonoids and tannins). The CEs from E. uniflora showed higher amount of polyphenols (30.35 ± 2.15%, AC-W) and the HPLC analysis revealed the tannins in all extracts. The CEs of E. uniflora showed MIC range from 1.9 to 500.0 µg/mL, and lower values of MIC50 and MIC90 against non-albicans Candida isolates. Regarding L. ferrea and P. guajava, the results showing MIC from 3.9 to 1000.0 µg/mL (CE-AQ) against C. albicans. The results demonstrate antifungal performance from CE against various species of Candida spp., suggesting that the herbal species may be use as new potential antifungal agents. Additionally, the polyphenol content can play a pivotal role in the antifungal properties of CE.


Subject(s)
In Vitro Techniques/methods , Plant Extracts/adverse effects , Polyphenols/analysis , Phytochemicals , Antifungal Agents/administration & dosage , Flavonoids/pharmacokinetics , Microbial Sensitivity Tests/methods , Chromatography, High Pressure Liquid/methods
8.
Braz. dent. sci ; 23(4): 1-7, 2020. tab
Article in English | LILACS, BBO | ID: biblio-1121326

ABSTRACT

Objective: Oral candidiasis is an infection that occurs in the oral cavity and is caused by candida species, often Candida albicans. This infection commonly occurs in a condition of immunosuppression caused by dexamethasone. Due to the side effects of antifungal therapy, developing a standardized immunosuppressed animal model to induce oral candidiasis for new therapies is required. The aim of this study is to observe oral candidiasis in immunosuppressed Wistar rats post dexamethasone injection at 7.2 mg/kg and 16 mg/kg doses. Material and Methods: Twenty-one Wistar rats were divided into three groups: control group, treatment group 1 (injected with dexamethasone at a concentration of 7.2 mg/kg), and treatment group 2 (at a concentration of 16 mg/kg) for five days. Immunosuppression status was observed by leukocyte count and all the subjects' palates were inoculated with C. albicans 0.1 ml of 15x108 UFC/ml 24 hours later. The subjects' tongues were observed and confirmed by laboratory examination on day 10. A statistical analysis was performed using one way ANOVA, Kruskal­Wallis, Tukey HSD, and Mann-Whitney U tests. Results: A significant clinical appearance of the subjects' tongues was observed only between C and T1 (p=0.023; p<0.05). Significant hyphal formation was observed between C and T1 (p= 0.037; p<0.05) and between C and T2 (p=0.007; p<0.05), and no significant difference was observed between T1 and T2. A significant increase in the colony count was also observed in similar results. Conclusion: Dexamethasone injection at doses of 7.2 mg/kg and 16 mg/kg is effective in triggering immunosuppression to induce oral candidiasis in immunosuppressed Wistar rats. (AU)


Candidíase oral é uma infecção que ocorre na cavidade oral e é causada por espécies de Candida, freqüentemente Candida albicans. Esta infecção geralmente ocorre em uma condição de imunossupressão causada por dexametasona. Devido aos efeitos colaterais de terapia antifúngica, o desenvolvimento de um padrão de modelo animal imunossuprimido para induzir candidíase oral para testar novas terapias se faz necessário. O objetivo deste estudo é observar candidíase oral em ratos Wistar imunossuprimidos pós-injeção de dexametasona utilizando doses de 7,2 mg /kg e 16 mg / kg. Material e métodos: Vinte e um ratos Wistar foram divididos em três grupos: grupo controle, tratamento grupo 1 (injetado com dexametasona na concentração de 7,2 mg / kg), e tratamento grupo 2 (concentração de 16 mg / kg) por cinco dias. A imunossupressão foi observada pela contagem de leucócitos e todos os palatos dos animais foram inoculados com C. albicans 0,1 ml de 15x108 UFC / ml após 24horas. As línguas dos animais foram observadas e confirmadas por exame laboratorial após 10 dias. A análise estatística foi realizada usando os testes de ANOVA um fator, kruskal ­ Wallis, Tukey HSD e de Mann-Whitney. Resultados: Significante diferença na aparência clínica da língua dos animais foi observada apenas entre C e T1 (p = 0,023; p <0,05). Significativa diferença na formação de hifas foi observada entre C e T1(p = 0,037; p <0,05) e entre C e T2 (p = 0,007; p <0,05), e nenhuma diferença significativa foi observada entre T1 e T2. Um aumento significativo na contagem de colônias foi também observado com resultados semelhantes. Conclusão: Injeção de dexametasona utilizando doses de 7,2 mg / kg e 16 mg / kg é eficaz no desencadeamento da imunossupressão para induzir candidíase oral em ratos Wistar imunossuprimidos. (AU)


Subject(s)
Rats , Candidiasis, Oral , Dexamethasone , Immunosuppression
9.
Rev. patol. trop ; 49(2): 105-119, 2020. ilus, tab, graf
Article in English | LILACS | ID: biblio-1118279

ABSTRACT

This study aimed to identify Candida spp. collected from oral mucosa and maintained in culture collections, correlating the findings with the medical history of patients and comparing with data from the literature over the past five years. Seven hundred and eleven oral Candida spp. isolates, collected between 2013 and 2017, were selected and identified using traditional and molecular methods. In addition, a literature review was performed with the key words: "Oral", "Candida" and "Yeast". Seven species of the genus Candida: were identified C. albicans(73.3%); C. tropicalis (9.3%); C. parapsilosis (8.2%); C. glabrata (3.9%); C. guilliermondii(2.8%); C. krusei (1.7%) and C. lusitaniae (0.3%). The strains identified as C. albicans were submitted to molecular methods using specific primers and of these, 5.8% were identified as C. dubliniensis strains. The greatest diversity of strains was found in patients presenting no systemic diseases or HIV +, while the highest percentage of strains of Candidanon-albicanswere observed in cancer patients. This study reports a representative distribution of Candidaspecies among individuals exhibiting distinct clinical conditions, in order to contribute to the design of future research on details of aspects involved in the infections caused by these microorganisms. The correct identification of oral Candida strains contributes to a realistic epidemiological approach and future clinical protocols against these pathogens


Subject(s)
Candida , HIV , Mouth Mucosa , Neoplasms
10.
Article | IMSEAR | ID: sea-210154

ABSTRACT

Background: Candida infections are known contributors to the high morbidity and mortality rates seen in HIV positive patients.Methodology: This was a descriptive cross-sectional study. The study was carried out at the Microbiology research laboratory, department of Medical Microbiology, University of Abuja Teaching Hospital, Gwagwalada. The population is made up of two hundred and ten (210) patients who presented with oral thrush between fifteen years and seventy years which comprises of 160 HIV seropositive and 50 non age and sex matched HIV seronegative patients. Culture, Microscopy and ELISA methods were used for isolation of Candida albicans. An interviewer-administered, structured questionnaire was used as the study tool Results: The mean age for the isolation of Candida albicanswas 30 ± 18.7 years, with the highest proportion of isolates within the age range of 21-30 years accounting for 27.1% of the study population recruited and the lowest proportion of isolates being 41-50 years accounting for 8.0%.From the 210 subjects withCandida albicansin the study, one hundred and sixty (160) representing 76.2 were isolated from HIV seropositive clients and Candida albicansisolation rate among HIV seronegative population was 23.8%.Conclusion: The sensitivity, specificity and positivepredictive value of using Grams reaction methods in the diagnosis of Candida albicanswas 22.9%, 95.2% and 82.6%. The sensitivity, specificity and positive predictive value of using ELISA methods was 25.7%, 86.7% and 65.9%.In this study, there was preponderance of Candida albicansisolate among the young and the old in HIV seropositive patients but largely isolated from older patients among HIV seronegative patients

12.
Article in Chinese | WPRIM | ID: wpr-750457

ABSTRACT

@#Oral infectious diseases include caries, periodontal disease, halitosis, candidiasis albicans and so on. Over the past few decades, probiotics have mainly been studied in the field of the gastrointestinal tract. In recent years, probiotics have begun to be used in the prevention and treatment of various oral diseases and have become a new field in the research of oral disease prevention and control technology. This paper reviews the research progress of probiotics applied in the prevention and treatment of various oral infectious diseases. A review of the literature shows that probiotics can prevent and cure dental caries by inhibiting the growth of Streptococcus mutans and competing with them for nutrition and attachment sites. Probiotics not only inhibit periodontal pathogens and reduce the production of sulfide, they also regulate the body’s immune function to alleviate halitosis and periodontal inflammation. Probiotics can inhibit periodontal caries by inhibiting them. Probiotics can inhibit them mycelial growth of Candida albicans and interfere with its adherence, thus playing a role in the prevention and treatment of oral candidiasis. Current studies have shown that probiotics play an auxiliary role in the treatment of caries, periodontitis, halitosis and oral candidiasis. However, the mechanism of probiotics in the prevention and treatment of oral infectious diseases is still unclear, and the safety of probiotics remains to be further studied. In the future, oral probiotics should be studied with reference to intestinal probiotics to better work to prevent and treat oral diseases.

13.
Article in English | WPRIM | ID: wpr-750350

ABSTRACT

@#Oral candidiasis is an opportunistic infection of the oral cavity. This study aimed to determine prevalence of oral candidiasis in patients with palatal coverage orthodontic appliances and prostheses and its risk factors. Three groups of patients were recruited after an informed consent. The two tested groups were the denture and the upper removable orthodontic appliance (URA) groups while patients with no prosthesis/appliance acted as control. The assessments included demographic profile, denture/URA age, night-time wearing and duration of wearing. Signs and symptoms of candidiasis were examined intra-orally. Unstimulated salivary flow rate test was also performed. Smears samples were taken from palatal mucosa and impression surfaces of denture/URA and stained with Periodic Acid Schiff (PAS). The data were analysed using descriptive and chisquare tests. A total of 86 patients were recruited; denture (n=30), URA (n=22), control (n=34). Denture and URA groups had significantly more positive cases of candidal infection (56.7 and 72.7% respectively) as compared to control group (20.6%). Among the subjects with oral candidiasis, denture patients were detected to have more severe amount of candidal hyphae (20%) than URA (13.6%) group. The relationships between oral candidiasis and these risk factors i.e. age, night-time wearing, daily total hours of wearing and denture/URA hygiene were statistically significant (p<0.05). Thus, patients who are wearing palatal coverage removable appliances or prostheses have higher prevalence of oral candidiasis. The risk is greater with confounding factors such as age, duration of wearing and oral hygiene

14.
Chinese Journal of Epidemiology ; (12): 1003-1005, 2019.
Article in Chinese | WPRIM | ID: wpr-805756

ABSTRACT

Objective@#To investigate the prevalence of oral mucosal diseases (OMD) in patients with cerebrovascular disease.@*Methods@#A total of 182 patients with cerebrovascular disease and 166 controls were examined for OMD to compare the differences of prevalence rates.@*Results@#The prevalence of OMD in patients with cerebrovascular disease appeared higher than that in the control group. Oral candidiasis was most commonly seen (11.1%, 20/182), followed by fissured tongue (5.0%, 9/182), traumatic ulcer (2.8%, 5/182), herpes labialis (2.2%, 4/182), recurrent oral ulcer (1.6%, 3/182), chronic cheilitis (1.6%, 3/182) and oral leukokeratosis (1.6%, 3/182).@*Conclusion@#Patients with cerebrovascular diseases were susceptible to OMDs, especially to oral candidiasis that called for more attention.

15.
Rev. Ciênc. Méd. Biol. (Impr.) ; 17(3): 315-321, nov 19, 2018. tab, ilus
Article in Portuguese | LILACS | ID: biblio-1247693

ABSTRACT

Introdução: a candidíase é uma infecção fúngica oportunista, causada pela proliferação e disseminação de espécies de Candida, que pode acometer a cavidade oral. Dentre os antifúngicos mais utilizados e de uso tópico, a nistatina é considerada o medicamento de primeira escolha. Objetivo: avaliar as propriedades físico-químicas de diferentes marcas de nistatina disponíveis no mercado, incluindo o pH, a acidez total titulável (ATT) e a determinação de sólidos solúveis totais (SST). Metodologia: trata-se de um estudo experimental in vitro, constituído por uma amostra de oito diferentes marcas de nistatina em suspensão oral de uso tópico. Foi analisado o potencial erosivo e cariogênico dessas soluções mediante a determinação de pH, ATT e SST (°Brix). Resultados: no tocante ao pH, verificou-se que a média obtida foi de 6,05 (± 0,66). Dois dos medicamentos analisados (marcas A e H) apresentaram pH abaixo do crítico para a dissolução do esmalte dental. Quanto à ATT das soluções, os valores variaram de 1,9 a 14,53 mL para atingir o pH neutro, indicando que as marcas B, C e E podem levar mais tempo para ser neutralizadas em razão da quantidade de solução necessária. A análise do °Brix revelou que a marca H apresentou o maior teor de açúcares em sua composição (44,9%). Conclusão: a formulação de nistatina da marca H apresentou pH endógeno mais crítico e percentual de sólidos solúveis totais elevado, sendo, portanto, a medicação com maior fator de risco para o desenvolvimento de cárie e erosão dentária, devendo ser consideradas as doses e frequências de uso, bem como os hábitos de higiene oral do paciente


Introduction: candidiasis is an opportunistic fungal infection caused by the proliferation and spread of Candida species that can affect the oral cavity. Among the most commonly used topical antifungal agents, nystatin is considered the first choice drug. Methodology: to evaluate the physical and chemical properties of different brands of nystatin available in the market, including pH, titratable acidity and determination of total soluble solids. Results: Regarding pH, it was verified that the mean obtained was 6.05 (± 0.66). Two of the analyzed drugs (A and H) presented pH below that considered critical for the dissolution of dental enamel. As for the titratable total acidity of the solutions, values ranged from 1.9 to 14.53 mL to reach neutral pH, indicating that the B, C and E marks may take longer to neutralize because of the amount of solution required. The analysis of ° Brix revealed that the H mark had the highest sugar content in its composition (44.9%). Conclusion: Nystatin brand H presented the worst indices in terms of endogenous pH and total sugar percentage, being therefore the medication with the highest risk factor for the development of caries and dental erosion.


Subject(s)
Tooth Erosion/chemically induced , Candidiasis, Oral/drug therapy , Cariogenic Agents/analysis , Nystatin/adverse effects , Antifungal Agents/adverse effects
16.
J. oral res. (Impresa) ; 7(8): 391-394, nov. 30, 2018. ilus
Article in English | LILACS | ID: biblio-1121123

ABSTRACT

Pseudomembranous candidiasis is the most frequent type of infection by candida spp., and candida albicans is the most common species to cause it. candidiasis can be due to other candida species less frequently, as is the case of candida tropicalis a pathogenic species that can cause infection in immunocompromised patients. the aim of this case report is to describe a pathological condition produce by candida tropicalis.


Subject(s)
Humans , Male , Adult , Candidiasis , Candidiasis, Oral/etiology , Candida tropicalis , Candida albicans , HIV
17.
Rev. chil. infectol ; 35(4): 377-385, ago. 2018. tab
Article in Spanish | LILACS | ID: biblio-978048

ABSTRACT

Resumen Introducción: Se desconocen los factores asociados a la candidiasis oral en población pediátrica con infección por VIH de los países en desarrollo. Objetivo: Identificar los factores asociados a la colonización por Candida, candidiasis oral y la susceptibilidad in vitro a antifúngicos, en niños y adolescentes con infección por VIH institucionalizados en la ciudad de Tijuana, México. Materiales y Métodos: Se examinó la cavidad oral de 30 niños y adolescentes con infección por VIH, se obtuvo una muestra de la mucosa oral para identificar las especies de Candida mediante cultivo y auxonograma. La susceptibilidad a los antifúngicos se determinó de acuerdo al CLSI. Los indicadores del estado inmunológico y falla virológica se clasificaron conforme a la OMS. Resultados: Se identificaron seis especies de Candida, 53% colonizantes y 47% causantes de candidiasis. Los factores asociados a candidiasis fueron alta carga viral (p = 0,001), menor recuento de LTCD4+ (p = 0,002) y esquema TARAA (p ≤ 0,014). La especie prevalente fue C. glabrata (33%); sin embargo, C. albicans (27%) fue más resistente a fluconazol (p = 0,001). Las especies resistentes a itraconazol se identificaron en esquemas que incluyen un INNTR (p = 0,041). Conclusiones: Los niños y adolescentes con infección por VIH institucionalizados mostraron una prevalencia elevada de Candida spp. colonizante y resistencia a los antifúngicos relacionada con los INNTR .


Background: Factors associated with candidiasis and colonization in HIV-positive children and adolescents in developing countries are not well understood. Aim: To identify the factors associated with oral Candida colonization and candidiasis in institutionalized HIV-positive children and adolescents in Tijuana, México, as well as the response of the isolates to antifungals. Materials and Methods: Sample of the oral mucosa of 30 HIV positive children and adolescents were obtained to isolate and identify Candida species by culture and metabolic profile. Antifungal drugs susceptibility was determined according to CLSI. Indicators of immunological and virologic failure were classified in accordance to WHO criteria. Results: Six Candida species were identified from oral mucosa, 53% colonizers and 47% in candidiasis. Factors associated with candidiasis and oral colonization were viral load (p = 0,001), CD4+ counts (p = 0,002) and HAART regimen (p ≤ 0,014). The most prevalent species was C. glabrata (33%), but C. albicans (27%) was more resistant to fluconazole (p = 0,001). Itraconazol resistant species were identified in regimens that include an NNRTI (p = 0,041). Conclusion: HIV-positive children and adolescents living in an orphanage showed high prevalence of colonizing Candida spp. and resistance to antifungals, related to NNRTI.


Subject(s)
Humans , Male , Female , Infant , Child, Preschool , Child , Adolescent , Adult , Young Adult , Candida albicans/isolation & purification , Candidiasis, Oral/microbiology , HIV Infections/complications , AIDS-Related Opportunistic Infections/microbiology , Mouth Mucosa/microbiology , Candida albicans/classification , Candidiasis, Oral/classification , Candidiasis, Oral/drug therapy , Fluconazole/therapeutic use , HIV Infections/drug therapy , Cross-Sectional Studies , Prospective Studies , Risk Factors , AIDS-Related Opportunistic Infections/drug therapy , Itraconazole/therapeutic use , Viral Load , Drug Resistance, Fungal , Mexico , Antifungal Agents/therapeutic use
18.
Rev. argent. dermatol ; 99(2): 1-10, jun. 2018. ilus
Article in Spanish | LILACS-Express | LILACS | ID: biblio-957915

ABSTRACT

HTLV-1 es un retrovirus endémico en Perú, relacionado ocasionalmente con algunas infecciones oportunistas aisladas. Presentamos el caso de un varón de 41 años, avicultor, con antecedente de TBC pulmonar tratado hace 6 meses. Ingresó a emergencia por presentar alteración de conciencia, disartria y diarrea acuosa. Al examen físico se evidenciaron placas confluentes en cavidad oral, lesiones máculo-papulares violáceas y placas costrosas; por biopsia de piel se confirmó sarcoma de Kaposi y sarna costrosa, además adenopatías cervicales que al estudio microscópico con test de Auramina mostró BAAR (++) y en el examen de heces con tinción Zielh Nielsen modificado, se evidenció ooquiste de Cystoisospora belli. Recibió trimetropin / sulfametozaxol, tratamiento antituberculoso. Se confirmó HTLV-1 por inmunofluorescencia. En el fondo de ojo se observó retinitis por citomegalovirus, recibió ganciclovir. A las tres semanas del ingreso hospitalario, falleció por insuficiencia respiratoria severa. Se discute la presencia de múltiples co-infecciones oportunistas en un paciente con inmunosupresión por HTLV-1.


HTLV-1 is an endemic retrovirus in Peru , occasionally associated with some isolated opportunistic infections. We present the case of a 41-year-old male poultry farmer with a history of pulmonary tuberculosis treated 6 months ago. He was admitted to emergency due to alteration of conscience, dysarthria and watery diarrhea; the examination revealed confluent plaques in the oral cavity, violaceous maculopapular lesions and crusted plaques. Skin biopsy confirmed Kaposi's sarcoma and crusted scabies; in addition, cervical lymphadenopathies showed evidence of BAAR (++) in the microscopic study with Auramine test, and in the examination of feces with modified Zielh Nielsen's stain, Cystoisospora belli oocyst was observed, and trimetropin / sulfametozaxol received antituberculous treatment. HTLV-1 was confirmed by immunofluorescence. In the fundus of the eye cytomegalovirus retinitis was evidenced, he received ganciclovir. At three weeks of hospital admission he died due to severe respiratory failure. We discuss the presence of multiple opportunistic co-infections in a patient with immunosuppression by HTLV-1.

19.
Araçatuba; s.n; 2018. 186 p. ilus, tab, graf.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1009528

ABSTRACT

O objetivo deste estudo foi avaliar a atividade antimicrobiana/antibiofilme e a viabilidade celular de nanopartículas de prata (NPsAg) obtidas por uma síntese 'green' associadas ou não ao ß-glicerofosfato de cálcio (GPCa) contra Streptococcus mutans e espécies de Candida (cepas de referência e isolados clínicos orais incluindo cepas resistentes ao fluconazol). O efeito destes nanocompostos em combinação com o tirosol (TIR), fluconazol (FLC), nistatina (NIT) e anfotericina B (AnB) também foi avaliado. Além disso, nós avaliamos comparativamente as alterações do transcriptoma de células de C. glabrata CBS138 após exposição às NPsAg e ao Íon prata (Ag+ ). Inicialmente, as NPsAg foram sintetizadas por meio da redução do nitrato de prata com extratos de diferentes partes (casca, folha e semente) de uma romã (Punica granatum L.) associadas ou não ao GPCa. A Concentração Inibitória Mínima (CIM) e as Concentrações Fungicida/Bactericida Mínima (CFM e CBM) dos nanocompostos (NPsAg e NPsAgGPCa) associados ou não ao TIR contra S. mutans e C. albicans foram determinadas pelo método de microdiluição. E, a ação das NPsAg combinadas com FLC, NIT e AnB também foi avaliada em condição planctônica contra isolados clínicos orais de espécies de Candida. O efeito dos nanocompostos associados ou não ao TIR sobre a viabilidade celular de fibroblastos da linhagem L929 e a produção de citocinas foi avaliado através dos ensaios de MTT e ELISA, respectivamente. O número de hifas foi quantificado em biofilmes de C. albicans formados na presença das NPsAg com ou sem soro fetal bovino (SFB). O efeito das NPsAg em inibir a formação do biofilme (em C. albicans e C. glabrata) também foi investigado através do ensaio de PrestoBlue e por meio da microscopia eletrônica de varredura. Os nanocompostos (NPsAg e NPsAg-GPCa) associados ou não ao TIR foram aplicados sobre biofilmes de S. mutans e de espécies de Candida (12 e 24 h) e, após 24 h de contato, sua atividade antibiofilme foi determinada por meio da enumeração das unidades formadoras de colônias (UFCs) e ensaio de PrestoBlue. Além disso, uma análise transcriptômica foi realizada utilizando microchips de DNA ('microarrays') a fim de avaliar quais genes estão mais ou menos expressos como resposta às NPsAg (no estado planctônico e formando biofilmes) e ao Íon Ag+ (formando biofilmes). As soluções antimicrobianas sintetizadas neste estudo (NPsAg e NPsAgGPCa) apresentaram atividade antimicrobiana contra os microrganismos testados. Além disso, menores valores de CIM foram obtidos quando estes nanocompostos foram associados ao TIR, FLC, NIT e AnB apresentando um efeito sinérgico. NPsAg e NPsAgGPCa não foram tóxicas às células L929, aumentaram a produção de fator de crescimento celular e não promoveram alterações significativas na liberação de Interleucina-6. Os nacompostos associados ao TIR não apresentaram efeito citotóxico sobre culturas de L929, exceto para a maior concentração (NPsAg ­ 39,05 µg/mL + TIR ­ 1,25 mM). A presença das NPsAg reduziu drasticamente o número de hifas em biofilmes de C. albicans na presença ou na ausência de SFB. A quantidade de biofilme das espécies de Candida formado na presença das NPsAg reduziu para mais de 50%. Após 24 h de tratamento com os nanocompostos (NPsAg e NPsAg-GPCa) em biofilmes de S. mutans, houve uma redução significativa no número de UFCs sendo similar à clorexidina. Uma redução na viabilidade de biofilmes de C. glabrata também foi observada após exposição às NPsAg (24 h). Entretanto, os nanocompostos (NPsAg e NPsAg-GPCa) associados ou não ao TIR não foram efetivos contra biofilmes de C. albicans. Após exposição às NPsAg e ao Íon Ag+ , alterações no transcriptoma de células de C. glabrata CBS138 foram observadas: no estado planctônico, houve uma superexpressão dos genes responsáveis pela biossíntese de metionina e de lisina e uma subexpressão dos genes relacionados com o transporte transmembrana; e, as células expostas às NPsAg responderam de forma distinta em relação ao Íon Ag+ , indicando que as NPsAg podem apresentar um mecanismo de ação diferente. Estes achados podem auxiliar nas decisões terapêuticas com formulações contendo NPsAg ou NPsAg-GPCa associadas ou não a diferentes drogas em pacientes com cárie dentária e candidíase oral(AU)


The aim of this study was to evaluate the antimicrobial/antibiofilm activities and the cell viability of silver nanoparticles (AgNPs) obtained by a 'green' synthesis associated or not to ß-calcium glycerophosphate (CaGP) against Streptococcus mutans and Candida species (reference strains and oral clinical isolates including azole-resistant strains). The effect of these nanocompounds in combination with tyrosol (TYR), fluconazole (FLC), nystatin (NYT) and amphotericin B (AmB) also was evaluated. Furthermore, we evaluated, comparatively, the transcriptome alterations of cells of C. glabrata CBS138 after exposition to AgNPs and to silver ion (Ag+ ). Initially, AgNPs were synthesized by reducing silver nitrate with extracts of different parts (peel, leaves and seeds) of a pomegranate (Punica granatum L.) associated or not to CaGP. Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal/Bactericidal Concentrations (MFC and MBC) of the nanocomposites (AgNPs and AgNPs-CaGP) associated or not to TYR against S. mutans and C. albicans were determined by the microdilution method. And, the action of the AgNPs combined with FLC, NYT and AmB also was evaluated in planktonic condition against oral clinical isolates of Candida species. The effect of the nanocomposites associated or not to TYR on cell viability of fibroblasts (L929) and cytokines production was evaluated through MTT and ELISA assays, respectively. The number of cells undergoing filamentation was quantified in C. albicans biofilms formed in the presence of AgNPs with or without fetal bovine serum (FBS). The effect of AgNPs in inhibit the formation of biofilm (in C. albicans and C. glabrata) also was investigated through PrestoBlue assay and scanning electron microscope. Biofilms of S. mutans and Candida species (12 and 24 h) were treated with nanocomposites (AgNPs and AgNPsCaGP) associated or not to TYR for 24 h and, then, the viability of these biofilms was determined through PrestoBlue assay and colony forming units (CFUs). Moreover, a transcriptome analysis was performed using microarrays to determine which genes are up- or down-regulated as response to AgNPs (in the planktonic state and forming biofilms) and to Ag+ ion (forming biofilms). Antimicrobial solutions synthesized in this study (AgNPs and AgNPs-CaGP) presented antimicrobial activity against tested microorganisms. Furthermore, lower values of MIC were obtained when these nanocompounds were associated to TYR, FLC, NYT and AmB showing a synergistic effect. AgNPs and AgNPs-CaGP were not toxic to L929 cells, increased the stem cell factor production and did not promote significant alterations in the Interleukine-6 release. The nanocomposites associated to TYR did not present cytotoxic effect on L929 cultures, except for the higher concentration (AgNPs ­ 39.05 µg/mL + TYR ­ 1.25 mM). The incubation in the presence of the AgNPs drastically reduced the number of cells exhibiting hyphae, this effect being observed either in the presence or absence of FBS. The amount of biofilm formed by Candida species in the presence of the AgNPs was reduced by more than 50% the one formed in the absence of the nanoparticles, this reduction being further increased to more than 90% when the concentration of the AgNPs was increased. After 24 h of treatment with the nanocompounds (AgNPs and AgNPs-CaGP) in S. mutans biofilms, there was a significant reduction in the number of CFUs being similar to chlorhexidine. A reduction in the viability of C. glabrata biofilms also was observed after exposition to AgNPs (24 h). However, the nanocomposites (AgNPs and AgNPs-CaGP) associated or not to TYR were not effective against C. albicans biofilms. After exposition to AgNPs and to Ag+ ion, alterations in the transcriptome of cells of C. glabrata CBS138 were observed: in the planktonic state, genes responsible by the methionine and lysine biosynthesis are up-regulated, and genes related with the transmembrane transport are down-regulated. And, finally, the cells exposed to AgNPs responded differently in relation to the Ag+ ion, indicating that the AgNPs might present a different mechanism of action. All these results may help guide therapeutic decisions with formulation containing AgNPs or AgNPs-CaGP associated or not with different compounds in patients with dental caries and oral candidiasis(AU)


Subject(s)
Silver , Calcium Phosphates , Candidiasis, Oral , Dental Caries , Nanoparticles , Streptococcus mutans , Candida albicans
20.
Rev. Ciênc. Méd. Biol. (Impr.) ; 16(3): 318-322, dez 19, 2017. fig, tab, ilus
Article in Portuguese, French | LILACS | ID: biblio-1293100

ABSTRACT

Objetivo: avaliar a frequência de candidíase oral em pacientes portadores de câncer de cabeça e pescoço submetidos à radio e (ou) quimioterapia em um serviço de referência vinculado ao Sistema Único de Saúde do município de Salvador (BA). Metodologia: trata-se de um estudo seccional, realizado em uma amostra de conveniência e não probabilística de pacientes portadores de câncer de cabeça e pescoço submetidos à radio e (ou) quimioterapia. Foram coletados dados relativos às pessoas com candidíase oral, tais como sexo, idade e o tipo de tratamento a que foram submetidas (quimioterapia, radioterapia ou ambos), bem como a localização e o diagnóstico clínico do tipo de candidíase. Resultados: do total de 36 pacientes que se enquadraram nos critérios de inclusão, 16 apresentaram candidíase (44,4%). Onze indivíduos (68,75%) eram do sexo masculino e 07 (43,75%) possuíam faixa etária situada entre a quinta e a sexta décadas de vida. O tipo de candidíase prevalente foi a pseudomembranosa (87,5%), e o local mais acometido foi o palato duro (50%). Dos 16 pacientes com candidíase oral, 14 (87,5%) realizaram a radioterapia associada à quimioterapia, enquanto que 02 (12,5%) realizaram apenas a radioterapia. Conclusão: a candidíase oral é uma condição clínica muito comum nos pacientes portadores de neoplasias submetidos aos tratamentos antineoplásicos. A presença do cirurgião-dentista na equipe multidisciplinar que acompanha os pacientes oncológicos é relevante, pois poderá auxiliar na prevenção e no tratamento da candidíase e de outras condições clínicas.


Objective: to assess the frequency of candidiasis in patients with head and neck cancer submitted to radio and (or) chemotherapy in a referral service linked to the Unified Health System of the city of Salvador, Bahia. Methodology: this is a cross-sectional study consisting of a non-probabilistic sample of patients with oral candidiasis diagnosed in a convenience group. Data were collected on people with oral candidiasis, such as gender, age and type of treatment (chemotherapy, radiotherapy or both), as well as the location and clinical diagnosis of the type of candidiasis. Results: Oo the total of 36 patients that met the inclusion criteria, 16 presented candidiasis (44.4%). Eleven individuals (68.75%) were males and 07 (43.75%) had an age group between the fifth and sixth decades of life. The most prevalent type of candidiasis was pseudomembranous (87.5%) and the most affected site was the hard palate (50%). Of the 16 patients with oral candidiasis, 14 (87.5%) underwent radiotherapy associated with chemotherapy, while 02 (12.5%) only underwent radiotherapy. Conclusion: oral candidiasis is a very common clinical condition in patients with neoplasia submitted to antineoplastic treatments. The presence of the dental surgeon in the multidisciplinary team that follows up oncological patients is relevant, as it may help in the prevention and treatment of candidiasis and other clinical conditions


Subject(s)
Candidiasis, Oral
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