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1.
Article in Chinese | WPRIM | ID: wpr-907690

ABSTRACT

Objective:To establish the UPLC fingerprint analysis method for Puerariae Lobamle Radix. Methods:The column was Agilent ZORBAX Eclipse Plus C18 column (2.1 mm×100 mm, 1.8 μm). The mobile phase consisted of acetonitrile (A)-0.1% formic acid (b), gradient elution, flow rate was 0.3 ml/min. The detection wavelength was 250 nm. The column temperature was set at 30 ℃. The sample volume was 2 μl, and the similarity evaluation system of traditional Chinese medicine fingerprint was adopted. The cluster analysis, principal component analysis and partial least square method in SPSS software were used to judge the differences of Pueraria lobata from different habitats.Results:With puerarin as reference peak, 22 common peaks were calibrated, and 6 peaks were identified. The similarity of 25 batches of samples was above 0.990 except S22 was 0.935, which indicated that the samples were with good consistency. Through cluster analysis, principal component analysis and partial least square analysis, 25 batches of Puerariae Lobamle Radix can be clustered into 4-5 categories, and different components such as 3'-hydroxy puerarin were found. The extraction process of Puerariae Lobamle Radix was optimized based on analytic hierarchy process and multi-index orthogonal test. The results showed that adding 50% ethanol 40 ml and refluxing for 40 min was the best extraction process. Conclusion:UPLC fingerprint is suitable for Puerariae Lobamle Radix, and the results are reliable. It can be used as a quality evaluation method for Puerariae Lobamle Radix.

2.
Article in Chinese | WPRIM | ID: wpr-907614

ABSTRACT

Objective:To optimize the blending method of Shuanghuanglian injection, and to investigate its stability in different solvents (0.9% sodium chloride injection, 5% glucose injection, 10% glucose injection, glucose and sodium chloride injection). Methods:By using orthogonal test to optimize the best dissolution method of Shuanghuanglian injection By measuring the content change of insoluble particles, pH value and principal components (baicalin, forsythione, chlorogenic acid) in the finished products to investigatethe stability of Shuanghuanglian injection in different solvents. Results:The optimal blending method of Shuanghuanglian injection was to add 5 ml sterilized water for injection into the vial and oscillate at 1 200 r/min frequency for 5 min. The main constituents of Shuanghuanglian injection were stable in 8 h in the infusion of four kinds of finished products. Insoluble particles in 0.9% sodium chloride infusion and 5% glucose infusion met the requirements within 8 h, and insoluble particles in 10% glucose infusion and 6 h glucose and sodium chloride infusion met the requirements. The pH value of 0.9% sodium chloride infusion within 8 h met the optimal requirements of the best compatibility, 5% glucose infusion within 2 h met the requirements, and 4 h sodium chloride infusion met the requirements of the best compatibility. Conclusion:This study optimized the best preparation method of Shuanghuanglian (freeze-dried) for injection. Sodium chloride injection should be used as the solvent to prepare finished infusion in clinical application, and 5% glucose injection should be prepared just before use.

3.
China Pharmacy ; (12): 832-838, 2021.
Article in Chinese | WPRIM | ID: wpr-875816

ABSTRACT

OBJECTIVE:To optimize the extraction technology of Zhideke granules. ME THODS:The extraction technology (water extraction ,alcohol extraction ,water extraction and ethanol precipitation )of Zhideke granules was initially screened by ammonia-induced cough experiment and xylene-induced ear swelling experiment in mice. Based on its preparation route ,the immersion time of medicinal materials containing volatile oil was investigated with water absorption as index firstly. The single factor test was adopted to investigate the amount of water added and the extraction time taking the volatile oil yield as index to optimize the extraction technology of medicinal materials containing volatile oil. Taking the contents of irisflorentin and total flavonoids as indicators ,on the basis of single factor investigation ,orthogonal test was adopted to examine the influence of three factors including the amount of water added ,extraction time and extraction frequency ,so as to optimize the water extraction technology of Zhideke granules and the validation tests were conducted. RESULTS :The results of pharmacodynamics experiment showed that the cough latency of mice in water extract low-dose and high-dose groups (6.34,12.68 g/kg,by crude drug )and water-extraction alcohol-precipitation extract high-dose group (12.68 g/kg,by crude drug )were significantly longer than those inmodel group ,and the number of cough within 2 minutes was significantly reduced (P<0.05 or P<0.01). Compared with model group , the ear swelling of mice in water extract low-dose and high-dose groups (6.34,12.68 g/kg,by crude drug),ethanol extract high-dose group (12.68 g/kg,by crude drug) and water-extraction alcohol-precipitation extract hig dose group (12.68 g/kg,by crude drug ) were decreased significantly (P<0.05 or P<0.01). The swelling inhibition rates were 42.26%,55.08%,33.49%,51.56%,39.57% and 44.36% in low-dose and high-dose groups of water extract ,alcohol extract , water-extraction and alcohol-precipitation extract respectively ,indicating that the water extract had better antitussive and anti-inflammatory effects. The optimal extraction technology of volatile oil was adding 5-fold water ,soaking for 30 minutes,and extracting for 3 hours. The optimal water extraction technology was adding 12-fold water ,extracting for 3 times after soaked for 50 min,lasting for 1 h each time. Results of 3 times of validation tests showed that average content of irisflorentin in the extract obtained by optimal technology was 76.47 μg/g(RSD= 2.15%,n=3)and the average content of total flavonoids was 92.45 mg/g(RSD=0.48%,n=3). CONCLUSIONS :The optimal extraction technology of Zhideke granules is stable and feasible.

4.
China Pharmacy ; (12): 2327-2335, 2021.
Article in Chinese | WPRIM | ID: wpr-886912

ABSTRACT

OBJECTIVE:To optimize the form ulation of Zuojin pectin c apsules,and to prepare modern Zuojin pectin capsules with protective effects against gastric ulcers. METHODS :The formulation of Zuojin pectin capsules was optimized with orthogonal test with the contents of pectin ,soluble starch and dextrin as factors ,using formability ,moisture absorption and flow ability as indicators. Zuojin pectin capsule was prepared by wet granulation filling method with Zuojin extract powder as raw material. The contents of palmatine hydrochloride ,berberine hydrochloride ,evodiamine and rutaecarpin were evaluated by HPLC. Basket method was used to investigate the release behavior of the capsule in 0.1 mol/L HCl solution. The gastric ulcer model of rats was established by intragastric administration of 75% ethanol. Gastric ulcer index ,the inhibition rate of gastric ulcer and the pathological sections were used as indexes to investigate the protective effect of Zuojin pectin capsules (the doses were 54,108, 216 mg/kg)on gastric ulcer. RESULTS :The optimal formulation of Zuojin pectin capsules included 45% pectin,12% soluble starch,27% dextrin and 1% xylitol. Results of in vitro drug , release showed that palmatine hydrochloride and berberine, hydrochloride in Zuojin pectin capsules released 53.76% and No.54.82% respectively within 1 h,completely released at about 8 h, and conformed to the zero-order release behavior. 2492109374@qq.com Different doses of Zuojin pectin capsule could improve the ulcer injury of gastric tissue in gastric ulcer model rats to different extent ,and significantly reduced the gastric ulcer index(P<0.01),significantly increased the inhibition rate of gastric ulcer and the percentage of positive expression area of Schiff ’s iodate staining (P<0.01). CONCLUSIONS :Zuojin pectin capsule with protective effect on gastric ulcer and certain sustained- release effect is successfully prepared.

5.
China Pharmacy ; (12): 2223-2229, 2021.
Article in Chinese | WPRIM | ID: wpr-886803

ABSTRACT

OBJECTIVE:To establish the microwave processing technology of yellow wine-processed Curculigo orchioides , and compare it with traditional technology. METHODS :HPLC method was adopted to determine the contents of curculigoside , orcinol glucoside and orcinol gentiobioside in C. orchioides . Based on the single factor tests ,microwave processing technology was optimized and validated with orthogonal test combined with comprehensive weighted scoring method ,with the amount of yellow wine,microwave power ,wetting time and microwave time as factors ,using the contents of curculigoside ,orcinol glucoside , orcinol gentiobioside and ethanol soluble extract as the indexes. The contents of C. orchioides decoction pieces and processed products were compared. RESULTS :The optimal microwave processing technology included that the amount of yellow wine was 20%(the weight of C. orchioides decoction pieces was 20%),microwave power was 300 W,wetting time was 3 h,microwave time was 2 min. After 3 times of validation tests ,average contents of curculigoside,orcinol glucoside ,orcinol gentiobioside and ethanol soluble extract were 0.095 6%,0.723 9%,0.406 6%,10.115 3%,and RSD were 0.71%,0.54%,0.99%,1.44%(n=3). Average comprehensive score were 99.08(RSD=0.69%,n=3). Except for the content of ethanol soluble extract in traditional wine-processed product ,the contents of curculigoside and orcinol gentiobioside in traditional wine-processed product and microwave processed product as well as the content of ethanol soluble extract in microwave processed product were all significantly higher than C. orchioides decoction pieces ;the contents of curculigoside and orcinol gentiobioside in microwave processed product were both significantly higher than traditional wine-processed product (P<0.05). The contents of orcinol glucoside in 2 processed product were significantly lower than C. orchioides decoction pieces ,while the microwave processed product was significantly higher than traditional wine-processed product (P<0.05). CONCLUSIONS :Optimized microwave processing technology is stable and feasible ,and can be used for the processing of yellow wine-processed C. orchioides .

6.
Article in Chinese | WPRIM | ID: wpr-879013

ABSTRACT

On the basis of the previous work of the research group, the orthogonal design method was further used to optimize the processing technology for reducing toxicity of fried Tripterygium wilfordii in Lysimachia christinae Decoction. A total of 9 processed products of T.wilfordii in L.christinae decoction were prepared by four factors and three levels orthogonal design table. The contents of triptolide in T.wilfordii were determined by high performance liquid chromatography(HPLC) before and after processing: 4.27, 3.92, 3.57, 2.75, 2.42, 2.66, 3.51, 1.87, 1.75, 2.03 μg·g~(-1). On this basis, the above processed products were orally given to mice for 28 days. 12 hours after the last administration, food fasting except water was provided, and 24 hours later, the eyeballs were taken for blood and liver tissue. Serum biochemical indexes, liver lipid peroxidation and antioxidant related indexes were detected by kit method. Twenty-eight days after oral administration of raw T.wilfordii, the levels of serum alanine aminotransferase(AST), alanine aminotransferase(ALT), alkaline phosphatase(ALP) and liver malondialdehyde(MDA) in mice increased by 91%(P<0.01), 46%(P<0.05), 73%(P<0.01) and 99%(P<0.01), while the liver antioxidant indexes such as superoxide dismutase(SOD), glutathione(GSH), glutathione peroxidase(GPX) and glutathione-S transferase(GST) significantly decreased(P<0.01). After administration of the processed products, the above indexes were significantly reversed(P<0.01 or P<0.05). Especially, the processing conditions of A_3B_2C_1D_3 had the best detoxification effect on T.wilfordii, which decreased the high levels of AST, ALT, ALP and MDA by 49%(P<0.01), 32%(P<0.01), 42%(P<0.01), and 17%(P<0.05). Therefore, the best processing conditions for T.wilfordii in L.christinae decoction were A_3B_2C_1D_3, namely "15% mass fraction of L.christinae, 1 h moistening time, 160 ℃ frying temperature, and 9 min frying time".


Subject(s)
Animals , Antioxidants , Liver , Mice , Primulaceae , Technology , Tripterygium
7.
J Pharm Biomed Sci ; 2020 Feb; 10(2): 27-35
Article | IMSEAR | ID: sea-215710

ABSTRACT

Aim We want to increase the biological stability of short peptides by PEG modification. Methods Throughconnecting the maleimide group to one end of polyethylene glycol and adding a cysteine (Cys) to one end ofthe short peptide, the short peptide was finally modified by PEG through chemical bonds. We established areverse high-performance liquid chromatography (RP-HPLC) detection method to detect the change ofsubstances before and after the reaction; screened out the optimal detection method by orthogonal test;purified the modified short peptide by ultrafiltration; detected the reaction by infrared spectroscopy Changesin functional groups; tested the stability of RP-1 in rat plasma before and after modification. Result anddiscussion Through single factor test and orthogonal test, the pH in the reaction was 6, reaction temperaturewas 25 ˚C, reaction time was 1H, and reaction ratio was PEG: RP-1C 1:1.5. The solution does not contain RP-1Cafter ultrafiltration. Peripheral plasma stability testing found that the modified short peptides greatlyenhanced the stability. Conclusion Through experiments, we found the best conditions for the modification ofshort peptides, purification methods, and the stability of the modified short peptides was greatly improved.

8.
Article in Chinese | WPRIM | ID: wpr-799697

ABSTRACT

Objective@#This study takes hydroxypropyl-β-cyclodextrin (HP-β-CD) as the inclusion materials to optimize the preparation technic of tea tree oil (TTO) and evaluate its pharmaceutical performance.@*Methods@#Take the production rate of HP-β-CD tea tree oil inclusion and entrapment rate as the evaluation index, taking the orthogonal test method to optimize the production technic of tea tree oil (HP-β-CD inclusion and using infrared (IR), differential thermal scanning (DSC) method to characterize the inclusion compound to analyze the stability of TTO-HP-β-CD.@*Results@#The best technic to produce HP-β-CD tea tree oil is as follow: the ratio of TTO and HP-β-CD should be equal to 1/10, at 40 ℃, within 1 h. The average drug loading shoud be 9.25% ± 3.25%. The IR, DSC characterization results showed that the characteristic peak of tea tree oil disappeared after the microspheres, which indicated the HP-β-CD encapsulated the tea tree oil with good compatibility. In 80 ℃ water bath, the TTO-HP-β-CD was stable with the retention rate 40% after 8 h, the retention rate was 4.32 times than that of the unwrapped tea tree oil.@*Conclusions@#The HP-β-CD tea tree oil obviously has higher rate of inclusion and stability. Therefore, it’s worth to promoting and being used in the pharmacy preparations and cosmetics field.

9.
China Pharmacy ; (12): 2469-2472, 2020.
Article in Chinese | WPRIM | ID: wpr-829352

ABSTRACT

OBJECTIVE:To establish and optimize a extraction method of Fructus Gleditsiae Abnormallis ,and to analyze and identify chemical components of the extract simultaneously. METHODS :Fructus Gleditsiae Abnormallis was extracted with CO 2 supercritical fluid extraction (SFE)method. Based on single factor tests ,using extraction yield as index ,extraction temperature , extraction pressure and extraction time as investigation factors ,SFE technology was optimized with orthogonal test ,and validation test was performed. Chemical components in the extract were identified by GC-MS. Relative percentage of each component was calculated with area normalization method. RESULTS :The optimal SFE extraction technology of Fructus Gleditsiae Abnormallis was extraction temperature of 60 ℃,extraction pressure of 300 MPa and pression time of 15 min. Average extraction of 3 times of validation tests was 1.73%(RSD=1.78%,n=3). The 48 components in the extracts of Fructus Gleditsiae Abnormallis were identified,which accounted for 98.31% of the total amount of the extracts. The extracts of Fructus Gleditisae Abnormalis mainly included organic acids ,accounting for 36.99%,followed by alkaloids ,accounting for 12.59% in total. Main components were palmitic acid (16.62%),oleic acid (14.12%),N-aminotetrahydropyrrole(9.79%),2,6-dimethyloctane-1,7-dien-3-ol(5.95%), tetrahydropyran(3.83%),vanillin(3.39%),etc. CONCLUSIONS :SFE method of Fructus Gleditisae Abnormalis is established successfully,and the extract is mainly organic acids.

10.
Chinese Pharmacological Bulletin ; (12): 727-731, 2020.
Article in Chinese | WPRIM | ID: wpr-856981

ABSTRACT

Aim To establish a method for determining the antithrombotic biological activity of Honghua injection, which can be used to evaluate and control its quality. Methods Collagen-adrenalin was used to induce mouse acute cerebral thrombosis model and hemiplegic protection rate of Honghua injection in mice as an index to investigate the antithrombotic activity of Honghua injection. The experimental conditions of the administration dosage, inducer dosage, and different strains of mice were investigated, and the experimental conditions were optimized by orthogonal design. Results Honghua injection which was made by 5. 0 g crude drug · kg-1and continuously ip for 3 d, had obvious protective effect on collagen-adrenalin-induced acute cerebral thrombosis in mice. The established biological activity limit method showed a good repeatability, intermediate precision, and reproducibility. Each sample showed different degrees of differences in biological activity. Conclusions The thrombus test in mice can be used as a method for measuring the biological activity of Honghua injection. It is recommended to use 5. 0 g crude drug · kg-1dose as the limit for bioactive process optimization and product quality controlment of Honghua injection.

11.
Article in Chinese | WPRIM | ID: wpr-878786

ABSTRACT

Orthogonal experiments were used to optimize the process parameters of curcumin TPP-PEG-PCL nanomicelles; the particle size, electric potential and morphology under the electron microscope were systematically detected for the curcumin TPP-PEG-PCL nanomicelles; and the stability and in vitro release of the curcumin TPP-PEG-PCL nanomicelles were investigated. With DID fluorescent dye as the fluorescent probe, flow cytometry was used to study the uptake of nanomicelles by breast cancer cells, and laser confocal microscopy was used to study the mitochondrial targeting and lysosomal escape functions of nanomicelles. Under the same dosage conditions, the effect of curcumin TPP-PEG-PCL nanomicelles on promoting the apoptosis of breast cancer cells was evaluated. The optimal particle size of curcumin TPP-PEG-PCL nanomicelle was(17.3±0.3) nm, and the Zeta potential was(14.6±2.6) mV in orthogonal test. Under such conditions, the micelle appeared as regular spheres under the transmission electron microscope. Fluorescence test results showed that TPP-PEG-PCL nanomicelles can promote drug uptake by tumor cells, escape from lysosomal phagocytosis, and target the mitochondria. The cell survival rate and Hoechst staining positive test results showed that curcumin TPP-PEG-PCL nanomicelles had a good effect on promoting apoptosis of breast cancer cells. The curcumin TPP-PEG-PCL micelles can significantly reduce the mitochondrial membrane potential of breast cancer cells, increase the release of cytochrome C, significantly increase the expression of pro-apoptotic protein Bcl-2 and reduce the expression of anti-apoptotic Bax protein. These test results were significantly better than those of curcumin PEG-PCL nanomicelles and curcumin, with statistically significant differences. The results revealed that curcumin TPP-PEG-PCL nanomicelles can well target breast cancer cell mitochondria and escape from the lysosomal capture, thereby enhancing the drug's role in promoting tumor cell apoptosis.


Subject(s)
Apoptosis , Breast Neoplasms/drug therapy , Cell Line, Tumor , Curcumin/pharmacology , Humans , Lysosomes , Micelles , Mitochondria , Phosphatidylethanolamines , Polyethylene Glycols
12.
Article in Chinese | WPRIM | ID: wpr-823102

ABSTRACT

Objective To optimize the extraction process of main iridoid glycosides in Damnacanthus officinarum Huang and evaluate the antioxidant activity of Damnacanthus officinarum Huang in vitro. Methods The classical heating-reflux extraction method was selected. The volume fraction of ethanol, the volume of solvent and extraction time were taken as the evaluation factors. The comprehensive score of extraction yield and the monotropein content were used as the evaluation indexes. An orthogonal test was designed to select the best extraction conditions. The total reducing capacity, DPPH clearance rate and hydroxyl radical scavenging rate were measured to determine its antioxidant activity in vitro. Results The optimal extraction process was the reflux with 6 times volume of 60% ethanol for 2 hours. Damnacanthus officinarum Huang has certain antioxidant capacity, and the activity of ethyl acetate part had the best effect. Conclusion The optimized extraction process is stable and feasible, which can be used for extraction of the iridoid glycosides from Damnacanthus officinarum Huang. This study has proved that Damnacanthus officinarum Huang has certain antioxidant activity.

13.
China Pharmacy ; (12): 919-925, 2020.
Article in Chinese | WPRIM | ID: wpr-820838

ABSTRACT

OBJECTIVE:To optimize the water extraction and ethanol precipitation technology of Zhuang medicine Baijin granules. METHODS :HPLC method was adopted to determine the content of bergenin in Baijin granules extract. The extraction routes of Baijin granules (water decoction ,70% ethanol reflux extraction ,water decoction combined with 70% ethanol reflux extraction)was screened primarily with the yield of extract and the experiment of reducing uric acid of mice. The orthogonal test was adopted to optimize water extraction technology of Baijin granules with water multiple ,extraction time and extraction times as factors,taking the extraction yield and the bergenin content as index ,then the validation test was carried out. The orthogonal test was adopted to optimize the alcohol precipitation process of Baijin granules including the relative density of medicinal materials , alcohol content and the alcohol precipitation time ,then the validation test was carried out. By the experiment of reducing uric acid , the effects of medicinal materials extract of Baijin granules extract were compared before and after ethanol precipitation. RESLUTS:Established method for content determination of bergenin with linearity range of 0.007 2-0.288 mg/mL,had good precision,reproducibility,stability and accuracy. The initially chosen extraction process of Baijin granules was water decoction extraction. The optimal water extraction technology was soaked for 0.5 h,then decocted for 3 times with 14-fold water (mL/g)and 1.0 h each time. The optimal ethanol precipitation process was to concentrate the water extract to a relative density of 1.0 g/mL with alcohol content of liquid at 60% and precipitated for 12 h. Validation tests showed that RSDs of extract yield and beragenin content were all lower than 2%(n=3). The experiment of pharmacodynamics showed that water extract (before ethanol precipitation )and water extract after alcohol precipitation could significantly decrease the level of uric acid in hyperuricemia model mice (P<0.01). There was no statistical significance in the reduction of uric acid between 2 groups(P>0.05). CONCLUSIONS :The optimized water extraction technology can obtain good extract yield and bergenin content ,and combined with ethanol precipitation technology for removing excess impurities would not affect the pharmacodynamics. The water extraction and ethanol precipitation technology is feasible,and can be used for extracting the medicinal materials of Baijin granules and its edulcoration.

14.
Article in Chinese | WPRIM | ID: wpr-846525

ABSTRACT

Objective: To optimize the inclusion process for the volatile oil in Huaweishu Granules. Methods: Taking the ratio of volatile oil to β-cyclodextrin, inclusion temperature, stirring speed and stirring time as the factors, the inclusion process conditions were optimized by orthogonal test, the weight coefficients of each index were determined by entropy weight method, and the inclusion complex was verified by thin layer chromatography. Results: The inclusion rate of volatile oil obtained by stirring method was the highest, with the ratio of β-cyclodextrin to volatile oil of 10:1, the inclusion temperature was 40 ℃, the stirring time was 1 h, and the stirring rate was 400 r/min. Conclusion: The optimized volatile oil inclusion process is stable and feasible, with high inclusion rate of volatile oil and yield of inclusion compound.

15.
Article in Chinese | WPRIM | ID: wpr-790902

ABSTRACT

Objective To establish a method for determination and optimize the extraction process of the content of plantamajoside in plantain.Methods Plantamajoside content was determined by HPLC.The effects of ethanol concentration, ethanol amount and extraction time on the extraction of plantamajoside from plantain were studied by orthogonal design.Results The calibration curve was linear (r=0.999 6) over the range of 12.52-125.10μg/ml.The average recovery was 98.57% (RSD=1.45%).The optimum extraction process was as follows:60%ethanol, 10times volumes, extracted 2times, 1heach time.Conclusion The established method was simple, accurate and reproducible for determination of the content of plantamajoside in plantain.The optimal extraction process was stable and feasible.

16.
Article in Chinese | WPRIM | ID: wpr-802535

ABSTRACT

Objective: To investigate the removal effect of 5 kinds of common flocculants on 6 kinds of toxic alkaloids in processing wastewater of Aconiti Radix. Method: HPLC was employed to determine contents of 6 kinds of alkaloids in the processing wastewater of Aconiti Radix before and after flocculation.The mobile phase consisted of acetonitrile-tetrahydrofuran(25:15)-0.1 mol·L-1 ammonium acetate solution(each 1 L contains 0.5 mL of glacial acetic acid) for gradient elution, volume flow rate was 1.0 mL·min-1, detection wavelength was 235 nm.The removal characteristics and effects of five common flocculants of ferric chloride, aluminum sulfate, polymeric ferric sulfate, polyaluminum chloride/polyacrylamide(PAC/PAM) and sodium polyacrylate were compared and preliminarily optimized. Result: The 5 kinds of flocculants had certain selectivity for different types of alkaloids, and the removal of 6 kinds of alkaloids showed obvious differences.Among them, the PAC/PAM attenuated relative superiority, when the pH 6, adding amount of 0.25 g·L-1 and PAC-PAM dosing ratio of 30:1, comprehensive removal effect was relatively good, removal rates of mesaconitine and hypaconitine was 85.4%and 58.3%, respectively. Conclusion: The flocculation method can be used as a pretreatment process to reduce the toxicity of processing wastewater of Aconiti Radix.

17.
Article in Chinese | WPRIM | ID: wpr-802315

ABSTRACT

Objective:To optimize the processing technology of Moslae Herba processed with ginger juice, and to explore the changes of its volatile components in processing process. Method:The volatile components in Moslae Herba, ginger juice and Moslae Herba processed with ginger juice were extracted by steam distillation. Volatile components in these products were analyzed by HS-GC-MS and identified by NIST 11 standard mass spectra library. Gas chromatographic conditions were as following:HP-5MS elastic quartz capillary column(0.25 mm×30 m, 0.25 μm), helium as the carrier gas, flow rate of 1.0 mL·min-1, injector temperature at 250℃, sample quantity of 0.2 μL, split ratio of 50:1, temperature program for initial temperature at 40℃, up to 60℃ with the heating rate at 5℃·min-1, keep 2 min, up to 160℃ with the heating rate at 5℃·min-1, keep 3 min, finally rise to 250℃ with the heating rate at 25℃·min-1, keep it for 2 min and finish, mass spectrometry conditions were as following:electron impact ionization(EI), electron collision energy of 70 eV, ion source temperature at 230℃, the interface temperature at 280℃, quadrupole temperature at 150℃, no delay of solvent, electronic multiplier voltage at 2.188 kV, taking full scan mode, scanning range of m/z 35-550.Taking frying time, solid-liquid ratio and moistening time as factors, orthogonal test was adopted to optimize the processing technology with the comprehensive score of relative contents of thymol and carvacrol, number of volatile components and extracting amount of volatile oil as index. Result:A total of 27 volatile components were detected in Moslae Herba. There were 81 volatile components in Zingiberis Rhizoma Recens. The processed products of orthogonal test(No. 1-9) had 31, 38, 29, 35, 38, 33, 34, 22 and 26 volatile components, respectively. Extracting amount of volatile oil was in the order of Moslae Herba processed with ginger juice > Zingiberis Rhizoma Recens > Moslae Herba. The best processing technology was as following:moistening Moslae Herba with equal volume of ginger juice for 6 h, stir-frying for 8 min. Conclusion:Processing has certain impact on the extracting amount of volatile oil in Moslae Herba and the types of volatile components. This optimized technology is stable and feasible, which can provide experimental data for the quality evaluation of processed products of Moslae Herba, and lay a foundation for clarifying its processing mechanism.

18.
Article in Chinese | WPRIM | ID: wpr-800655

ABSTRACT

Objective@#To optimize the extraction technology for Gegen-Shujin granules.@*Methods@#With yield of volatile oil as index, single factor tests were used to investigate effects of water, soaking time and distillation time on extraction technology of volatile oil. The water amount, extraction time and numbers of extraction as factors, the contents of puerarin and total solid as indexes, orthogonal test was employed to optimize the extraction technology of Gegen-Shujin granules.@*Results@#The optimical extraction technology conditions were as follows: Cinnamomi Ramulus, cinnamomi ramulus, notopterygium, turmeric were extracted to get volatile oil with eight-folds amount water of herbs for 6 hours; while the other herbs were boiled with ten-folds amount water of herbs and extrancted for two times, 1.5 hours each time.@*Conclusions@#This extraction process is reasonable and practical, and can guarantee the quality of preparation.

19.
Article in Chinese | WPRIM | ID: wpr-845303

ABSTRACT

Objective: To establish a method for the determination of oleanolic acid(OA)in Pfaffia glomerata to compare the contents of OA in different parts of P. glomerata from Guangxi, China, with different growth years and different habitats. Methods: HPLC method was adopted. The determination was performed on Phenomenex Luna C18(250 mm×4.6 mm, 5 μm)column with mobile phase consisting of methanol-water-glacial acetic acid-triethylamine(285:15: 0.2:0.1, V/V) at the flow rate of 1.0 ml/min. The column temperature was set at 30℃. The detection wavelength was set at 210 nm, and the injection volume was 20 μl. The content of OA in P. glomerata was determined by the established method, and the extraction process of OA was optimized by the orthogonal test. Results: The linear range of OA was 0.0206-2.060 mg/ml(r2=1.0000). RSDs for the precision, repeatability and stability tests were all lower than 2%(n=6 or n=7). The average recovery of OA was 100.89%(RSD=1.69%, n=9). The optimum extraction conditions of OA were as follows: 20-fold ethanol(80%, V/V), extracting for three times, refluxing 1.5 hour each time, and processing acid hydrolysis with 3.0% sulfuric acid(g/g)for 1 hour. Under these conditions, the OA had the highest extraction efficiency. The contents of OA in reed head, root, old stem, tender stem, leaf and flower of P. glomerata were 4.87, 4.61, 2.67, 0.99, 0.24 and 1.13 mg/g, respectively. The average contents of OA in the roots of P. glomerata aged 1, 2, 3, 4 and 5 years in Guangxi were 3.08, 4.07, 4.71, 4.62 and 4.46 mg/g, respectively. Conclusion: The established extraction process and detection method is suitable for the extraction and content determination of OA in P. glomerata. Although OA is distributed in all parts of P. glomerata, the contents significantly vary in different parts. The content of OA is highest in reed head and lowest in leaves. The OA content in P. glomerata becomes stable after 3 years of growth in Guangxi.

20.
Article in Chinese | WPRIM | ID: wpr-845281

ABSTRACT

Objective: To optimize the preparation process of Schisandrae Chinensis Fructus granules(SCFG) to obtain a stabilized and qualified SCFG-preparation. Methods: The Schisandrae Chinensis Fructus raw materials were extracted by decoction with water,and the extraction process was optimized by the orthogonal test. In the orthogonal test, the yield of total extract and the extraction rate of schizandrin were used as evaluation index,and the effect of solid-liquid ratio,decoction time and extraction times on the indexes were investigated to optimize the related parameters of these fac- tors. Meanwhile,the concentration process,drying process and granulation process were also investigated to finally opti- mize the preparation process of SCFG products. The content of schizandrin was determined by the HPLC method. Re- sults Under the HPLC conditions in the Pharmacopia of the People’s Republic of China(2015 edition),schizandrin showed a good linearity in the range of 0.03598-0.28784 μg(r=1.0000),with the average recovery rate 99.32% and RSD 2.17%. The conditions for the optimized extraction process were 3 times of extraction,with 0.5 h of each decoction time and with the 1:8,1:6 and 1:6 solid- liquid ratio in turn. The conditions for the optimized concentration process were concentrating at 60℃ and -0.08 MPa vacuum to a certain concentration. The conditions for the optimized drying pro- cess were drying at 60℃ and -0.09 MPa vacuum to a dryness. The conditions for the optimized granulation process were adding appropriate amount of dextrin,using 90% alcohol as moistening agent,and sifting with 14 mesh sieve and desic- cating at 60℃. Conclusion: The improved method is feasible,simple,stable and suitable for large-scale production of SCFG products.

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