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J Genet ; 2020 Sep; 99: 1-11
Article | IMSEAR | ID: sea-215489


Genome sequence of Acinetobacter baumannii DS002 revealed the existence of seven contigs with features of indigenous plasmids. Of the seven contigs, three of them have shown size and sequence identity. They appeared to have been generated due to the unique recombination events leading to a large-scale recombination and sequence inversions. The rest of the indigenous plasmids have shown significant size variations and contained the genetic repertoire required for the detoxification of formaldehyde and biosynthesis of exopolysaccharides. Genetic modules encoding novel toxin–antitoxin systems were found in most of the plasmids to ensure their survival in the host. In some instances, the toxin and antitoxin coding sequences were found on two different plasmids promoting the cosegregation of these two plasmids into the daughter cells

Microbiology ; (12)1992.
Article in Chinese | WPRIM | ID: wpr-684189


In order to remove invasive plasmids from Shigella flexneri 2a 2457T and Shigella sonnei S7 based on the principle of plasmid incompatibility Ori and inc genes were amplified by PCR from S. flexneri 2a invasive plasmids, and then they were cloned into plasmid pMD18 T, resulting recombinant plasmids pMDori and pMDinc After S flexneri 2a 2457T and S sonnei S7 were transformed with pMDori or pMDinc respectively Invasive plasmids were removed from S flexneri 2a 2457T and S sonnei S7