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The current study aims to assess the content of coffee oil, diterpenes, caffeine, and chlorogenic acid in coffee beans, and to evaluate the effects of roasting degrees on bioactive compound levels in Coffea arabica and their associations with glycated hemoglobin levels and kidney function parameters in streptozotocin-induced diabetic rats. These constituents were quantified using Soxhlet, liquid–liquid extraction (LLE), and high-performance liquid chromatography (HPLC)-diode array detecto (DAD). The outcomes exhibited a positive correlation between levels of coffee oil and diterpenes with a roasting degree. However, it demonstrated a negative correlation between caffeine and chlorogenic acid with a roasting degree. The male rats were categorized into two sections: healthy (nondiabetic) and streptozotocin-induced diabetic groups. Rats have daily administrated 2 ml of coffee extract using an oral gavage subjected to different roasting degrees for 3 weeks. Fasting blood glucose (FBG), urea, and creatinine in serum were assayed at baseline and end of the experiment. The highest significant elevation (Pa = 0.016) in the mean urea and creatinine levels was noted in the diabetic group administrated with dark coffee extract whereas the lowest elevation was in the diabetic group administrated with green coffee extract. In conclusion, our study has shown that coffee roasting degree may have a potential negative effect on kidney function that may accelerate or exacerbate diabetic nephropathy in induced diabetic rats. A noteworthy change in urea levels was observed in diabetic rats treated with dark-roasted coffee, which might be attributed to the decline in antioxidant constituents: chlorogenic acids and diterpenes.
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A novel technique was developed for simultaneous quantification of clarithromycin (CLA) amoxicillin (AMO), and vonoprazan (VON) in a mixture using the reverse phase ultra-performance liquid chromatography (RP-UPLC) technique and validated as per International Council for Harmonization (ICH) guidelines as there was no literature published for its estimation by UPLC. The method was developed using an acquity UPLC system from waters corporation with Hibar Bis phosphonate C18 column (100 × 2.1 mm, 2 µm) at 35° C and tunable ultra-violet detector (TUV) with detection wavelength at 210 nm, has a run time of below 3 minutes. The mobile phase proportion of 60:40 of 0.1 N monobasic potassium phosphate buffer (pH 3.8) and acetonitrile at a flow velocity of 0.2 ml/ minute was utilized. Linearity was observed for CLA, AMO, and VON between the concentration ranges of 25–150, 25–150, and 1–6 µg/ml, respectively, and R2 was 0.999 for CLA, AMO, and VON. Accuracy and precision were within 2% of the coefficient of variation (RSD) for all drugs. The observed mean percentage recoveries for the CLA, AMO, and VON were determined to be 99.74%, 99.07%, and 99.8%, respectively. The stability of the approach was assessed using degradation studies by exposing it to acid, alkali, oxidizing agent, heat, Ultra Violet (UV) light, and water as per ICH guidelines.
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Background: Vault prolapse usually follows vaginal or abdominal hysterectomy and is usually associated with cystocele, rectocele and enterocele. Diagnosis is made clinically but magnetic resonance imaging can better detect enterocele than clinical finding.Methods: It was a prospective study over 3 years, so women presenting with vault prolapse, in which grading of vault prolapse was made clinically and by pelvic organ prolapse quantification (POPQ). Magnetic resonance imaging was performed for all cases to quantify prolapse and compare findings of MRI and POPQ with intraoperative findings of prolapse.Results: Mean age, parity, BMI were 58.4 years, 3.5, 22.71kg/m2 respectively. Most patients (75%) had vaginal hysterectomy while 25% had abdominal hysterectomy. Symptoms were pressure in lower abdomen and perineum (55%), bulge and feeling of mass descending in perineum (100%) and chronic constipation (60%). The type of prolapse was cystocele (100%), rectocele (100%), vault prolapse (100%) and enterocele (45%). MRI had agreement with intraoperative findings in 65% cases in cystocele, 45% cases in vault prolapse, 50% in rectocele but had much higher agreement of 88.8% for enterocele. While POPQ had higher agreement with intraoperative findings for cystocele (80%), vault prolapse (98%), rectocele (80%) but had poorer agreement for enterocele (66.6%).Conclusions: This study demonstrated that MRI was inferior in diagnosis of cystocele, rectocele and vault prolapse than POPQ but was superior in identification of enterocele than POPQ.
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There is a concern about perioperative bleeding with any surgical intervention in patient with congenital factor VII (FVII) deficiency. The optimum dosage regimen for recombinant activated factor VII (rFVIIa) use has been not clearly established. We report a woman with post hysterectomy vault prolapse management with congenital FVII deficiency who underwent obliterative vaginal procedure. A 50-year-women with congenital FVII deficiency was diagnosed as post hysterectomy vault prolapse and planned for partial colpocleisis. She was planned to monitor FVII activity and prothrombin time international normalized ratio (PT/NR) intraoperatively. At the start of surgery, FVII activity was 3% and PT was INR 35.3 with INR of 2.83. (0.8-1.2). Presuming the improvement of 25% activity, 5 mg (70 mcg/kg body wt.) rFVIIa was administered. The PT was improved to 28.4 sec, Surgery was successfully completed without unexpected bleeding. Post operatively there was no oozing hence FVII activity and PT-INR was not checked. An additional rFVIIa dose of 2mg was given prophylactically after 12 hours of surgery. The patient was discharged on the third day after surgery without postoperative complication. In this case, rFVIIa was used just twice and there were no bleeding events during the perioperative period. Previous reports suggested using 15-30 ?g/kg of rFVIIa before surgery and subsequent every 4-6 h in the first 24 h, then increasing the interval to 8-12 h. It is necessary to evaluate optimal dose of rFVIIa based on the risk and surgical invasiveness for each case. Our patient with congenital FVII deficiency uneventfully underwent obliterative vaginal procedure.
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Objective:To analyze the correlation between the expressions of human epidermal growth factor receptor 2(HER2)and carbohydrate antigen 153(CA153)and the technical parameter of acoustic palpation tissue imaging quantification(VTIQ)in patients with breast cancer.Methods:A total of 80 female patients with breast cancer admitted to The Third People's Hospital of Hefei from May 2018 to May 2020 were selected,including 14 cases at WHO stage Ⅰ,22 cases at WHO stage Ⅱ,31 cases at WHO stage Ⅲ and 13 cases at WHO stage Ⅳ.Another 53 female patients with benign breast diseases who were treated during the same period were selected as controls.At first,all patients underwent routine ultrasound examination,and then they entered the ultrasound VTIQ imaging mode to obtain the mean value of shear wave velocity(SWV).An immunohistochemistry was used to detect HER2 expressions in breast tissues,and Roche E411 electrochemiluminescence immunoassay analyzer was used to detect serum CA153 levels of them.Pearson method was used to analyze the correlation between serum CA153 levels and SWV mean values in patients with breast cancer.Results:Compared with benign patients,the SWV mean value of VTIQ technical parameter,serum CA153 level and HRR2 positive expression rate in patients with breast cancer were significantly higher,and the difference was statistically significant(F=39.107,78.353,P<0.05),respectively.Compared with patients at stages Ⅰ + Ⅱ of breast cancer,the SWV mean value of VTIQ technical parameter,serum CA153 level and HRR2 positive expression rate of patients at stages Ⅲ and Ⅳ of breast cancer significantly increased(t=2.685,3.556,8.326,10.455,P<0.05),respectively.Compared with patients at stage Ⅲ of breast cancer,the SWV mean value of VTIQ technical parameters,serum CA153 level and HRR2 positive expression rate of patients at Ⅳ stage of breast cancer were significantly higher(t=4.632,8.659,P<0.05),respectively.Compared with the SWV mean value of patients with HER2 negative expression of breast cancer,that of patients with HER2 positive expression of breast cancer was significantly higher(x2=59.751,P<0.05).There was a positive correlation between serum CA153 levels and SWV mean values in patients with breast cancer(r=0.501,P<0.05).Conclusion:The SWV mean value of VTIQ parameters is closely related to the expression levels of biomarkers HER2 and CA153 in patients with breast cancer.
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Objective:To investigate the correlation between liver stiffness and histopathological changes in a rat model of acute hepatitis using virtual touch tissue imaging quantification (VTIQ) technology.Methods:A total of 100 SPF-grade SD rats were randomly divided into 3 groups: control ( n=30), low-dose ( n=35), and high-dose ( n=35) groups. Acute hepatitis models were induced in the low-dose and high-dose groups using 400 mg/kg and 600 mg/kg of Thioacetamide (TAA), respectively. Liver stiffness parameters of the right median lobe and right lobe were measured using VTIQ technology, Mean-H and Mean-L represent the liver lobes with higher and lower liver stiffness measurments, respectively, while Mean represent the average of the measurements from both liver lobes. Comparative analyses of liver stiffness parameters were performed across three groups and between the two lobes of the liver. The correlations between the Mean values of liver stiffness and semi-quantitative histopathological data were investigated. Ten rats were randomly selected from each of the 3 groups to test the repeatability of VTIQ values before and after euthanasia with intraperitoneal anesthesia. Subsequently, 10 rats after euthanasia from each 3 group were randomly chosen to assess the repeatability of VTIQ measurements for inter-observer and intra-observer variabilities. Results:VTIQ results showed statistically significant differences in Mean, Mean-H, and Mean-L among the 3 groups (all P<0.01). The high-dose group had higher measurements compared to the low-dose and control groups, with significant intergroup differences (all P<0.01). Significant differences in Mean-H and Mean-L were observed between the two liver lobes in both low and high-dose groups (all P<0.01). The Mean value showed significant positive correlations with semi-quantitative histopathological data of hepatocellular edema, periportal inflammatory cell infiltration, macrophage proliferation, and bile duct proliferation ( r=0.391, 0.648, 0.577, 0.542; all P<0.01). Multivariate linear regression analysis indicated that hepatocellular edema, eosinophilic change, and bile duct proliferation significantly and positively predicted the Mean value (β=-0.278, -0.196, -0.333; all P<0.05). There were no significant differences of VTIQ measurements befor and after euthanasia (all P>0.05), with repeatability coefficients of 0.166, 0.182, 0.185 for Mean, Mean-H, and Mean-L, respectively. Post-euthanasia, inter- and intra-observer VTIQ differences remained non-significant (all P>0.05), with Mean, Mean-H, Mean-L coefficients of 0.114, 0.194, 0.165 and 0.206, 0.322, 0.268, respectively. Conclusions:VTIQ technology demonstrates potential clinical value in assessing a rat model of acute hepatitis, offering a new perspective for non-invasive evaluation of acute hepatitis. However, its clinical application requires further validation.
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ObjectiveTo quantitatively analyze and evaluate the content of rehabilitation policy for people with disabilities in China. MethodsThis study focused on ten national policies of disability rehabilitation issued from 2021 to 2023. It employed text mining techniques to process policy texts and constructed a policy modeling consistency index model for disability rehabilitation policies in China. The relevant policies were evaluated and analyzed quantitatively. ResultsThe disability rehabilitation policies in China were relatively comprehensive in terms of policy transparency, operational mechanisms and policy nature. However, there was still a need for optimization in terms of policy perspectives, target groups, incentive mechanisms, and other aspects. ConclusionThe overall quality of disability rehabilitation policy texts at the national level in China is relatively good. There is a need to further enhance the predictability of policy objectives, clarify the responsibilities and division of labor among various departments, and improve policy incentive mechanisms in future policy formulation, which will further refine China's disability rehabilitation policy system and contribute to high-quality development of the disability cause.
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Objective To evaluate the gender differences in fat water fraction(FWF)related to fat metabolism in supraclavicular region of neck with iterative decomposition of water and fat with echo asymmetry and least square estimation iron quantification(IDEAL-IQ)sequence quantitatively.Methods Twenty healthy female and twenty healthy male volunteers were selected for a MRI examination with IDEAL-IQ,then the FWF of R2*,brown adipose tissue(BAT)and white adipose tissue(WAT)were obtained by post-processing.The differences of FWF between the two groups were compared by Mann-Whitney U test.Results There was sig-nificant difference in the FWF of BAT and WAT between the two groups(P<0.05).The FWF of BAT in the female was higher than that the male,and the FWF of WAT in the male was higher than that the female,there was no significant difference in the R2*between the two groups(P>0.05).Conclusion IDEAL-IQ sequence can be used to evaluate the FWF in supraclavicular region of neck quantitatively,and classify BAT and WAT,then provide clinical according to the quantitative study of fat content.
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Objective To investigate the value of computer-assisted quantification of pulmonary embolism volume(PEV)in identifying mild-to-high-risk acute pulmonary embolism(APE).Methods We retrospectively enrolled 143 patients with suspected APE confirmed by computed tomography pulmonary angiography(CTPA)at Yan'an University Affiliated Hospital from January 2017 to December 2020.According to the 2018 Chinese Guidelines for Diagnosis,Treatment and Prevention of Pulmonary Thromboembolism,all the patients were divided into low-risk group(n=88)and mild-to-high-risk group(n=55).We collected the patients'basic demographic data,clinical manifestations,and serum levels of N-terminal-B type natriuretic peptide precursor(NT-proBNP)and D-dimer.Based on CTPA images,the degree of pulmonary thromboembolism was artificially evaluated to obtain the pulmonary artery occlusion index(PAOI).The thrombus was segmented using the pulmonary embolism detection tool based on digital lung,and PEV was calculated.We compared the differences in clinical and laboratory indicators and PAOI and PEV between the two risk groups.We analyzed the value of PAOI and PEV in identifying mild-to-high-risk APE using receiver operating characteristic(ROC)curves,and used Logistic regression analysis to identify independent risk factors in predicting mild-to-high-risk APE.Different models were established.Results Compared with the low-risk group,APE patients in the mild-to-high-risk group were older(P<0.05),had lower diastolic blood pressure(P<0.05),higher levels of D-dimer and NT-proBNP(P<0.05),lower levels of platelet count,arterial oxygen partial pressure and arterial carbon dioxide partial pressure(P<0.05),and higher levels of PAOI and PEV(P<0.001).ROC curve analysis showed that the area under the curve for PEV in identifying mild-to-high-risk APE was 0.809(95%CI:0.734-0.884),while that for PAOI was 0.753(95%CI:0.667-0.839).Logistic regression analysis showed that PEV and NT-proBNP were independent risk factors for mild-to-high-risk APE(P<0.05).Conclusion PEV and NT-proBNP are independent risk factors for mild-to-high-risk APE.
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Wide-Field Calcium Images (WFCI) directly reflect neuronal excitation, but their poor frame rate could be a drawback for time series analysis. This work was aimed at exploring the diagnostic capability retained by a time series obtained from calcium imaging data. To that purpose, we analyzed publicly available data from 2.88 hour continuous recordings of calcium images obtained from seven mice at different wake/sleep stages. Data were obtained from the Physionet portal and were submitted to Recurrence Quantification Analysis (RQA). The association between retrosplenial and parietal areas was also assessed. Nonlinear RQA analysis allowed to identify the right retrosplenial and parietal areas as particularly sensitive to changes in sleep walking condition. Specifically, our results suggested that the RQA feature lmean decreases in non-REM sleep_1 stage as compared to waking stage. Sleep (both sleep_1 stage and REM) apparently elicits an increase in the association between retrosplenial and parietal areas. Overall, these results suggest that RQA and association analysis are appropriate to assess modifications associated to changes in brain condition, in spite of the low sampling rate of WFCI signals(AU)
Las Imágenes de Calcio de Campo Ancho (Wide-Field Calcium Images, WFCI) reflejan directamente la excitación neuronal, pero su escasa resolución temporal pudiera resultar un impedimento para el análisis de series temporales. El presente trabajo tuvo por finalidad explorar la capacidad diagnostica que retiene una serie temporal extraída de imágenes de calcio. Para ello, se estudió una base de datos disponible en la red que contiene registros de 2.88 horas de duración de imágenes de calcio correspondientes a 7 ratones transgénicos a diferentes estadios de sueño/vigilia. Los datos fueron descargados del portal Physionet y sometidos a Análisis de Cuantificación Recurrente (Recurrent Quantification Analysis, RQA). La asociación entre las áreas retrosplenial y parietal derechas fue también evaluada. El análisis no lineal mediante RQA permitió identificar las áreas retrosplenial y parietal derechas como zonas particularmente sensibles a cambios en el estado de sueño/vigilia. Específicamente, nuestros resultados sugieren que el índice l mean se redujo en el estadio 1 de sueño no REM en comparación con el estado de vigilia. El estado de sueño, tanto REM como no-REM aparentemente induce un reforzamiento en la apreciación entre las áreas retrosplenial y parietal derechas. En su conjunto, estos resultados apuntan que el análisis de RQA y de asociación entre áreas son pertinentes para sensar las modificaciones asociadas a cambios en el estado del cerebro, a pesar de la baja resolución temporal de las señales WFCI(AU)
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Humans , NeurosciencesABSTRACT
A novel and simple hyphenated method was applied for the screening of alkaloids in S. flavescens. The approach combined easy sample extraction with an ultra-high-performance liquid chromatography (UPLC) and a high-resolution mass spectrometry (HRMS) in the data-dependent acquisition (DDA) method. With the optimized chromatographic program, ten alkaloids were screened. Among them, matrine, oxymatrine, oxysophoridine, sophocarpine, and oxysophocarpine were identified and quantified. The other five alkaloids were identified with accurate m/z value in MS1 and product ions in MS2. The quantification method was validated with specificity, linearity, recovery, and precision. The LOD and LOQ were determined. In twelve batches of collected S. flavescens roots, the identified ten alkaloids were all observed. The oxymatrine was the most abundant component, and sophocarpine was the least abundant in these samples. This comprehensive method is a powerful tool for the biochemical and clinical research of S. flavescens. It can also be adopted in resource utilization and related medicine quality control.
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Objetivo: Evaluar si la corrección simulada del prolapso apical determina la reducción del prolapso del compartimiento anterior y posterior, utilizando el sistema de cuantificación del prolapso de órganos pélvicos. Métodos: Estudio de corte transversal. La población estuvo constituida por pacientes acudieron al Hospital Universitario de Caracas con prolapso de órganos pélvicos estadio II o mayor con compromiso del compartimiento apical, entre 2021 y 2022. La selección de la muestra se realizó en forma no probabilística, secuencial. Se cuantificó el grado del prolapso antes y después de la simulación de soporte apical con la valva posterior de un espéculo de Graves. Se evaluaron los puntos Aa Ba Ap y Bp, antes y después de la simulación y la proporción de pacientes en quienes el estadio del prolapso anterior o posterior cambió. Resultados: Se incluyeron 100 pacientes, la mayoría con prolapsos estadio III y IV. Luego del soporte apical, los puntos del compartimiento anterior se corrigieron hasta un valor entre 0 y - 3 en la mayoría de los casos (p < 0,05) y los de la pared posterior cambiaron a - 3 en la mayoría de las pacientes (p < 0,05). El estadio del prolapso del compartimiento anterior cambió a un estadio II o menor en 99 % de los casos (p < 0,05) y en el posterior en el 100 % (p < 0,05). Conclusión: La simulación de soporte apical durante la cuantificación del prolapso demostró una corrección del prolapso del compartimiento anterior y posterior en un porcentaje elevado de pacientes(AU)
Objective: To evaluate whether simulated correction of apical prolapse determines the reduction or disappearance of anterior and posterior vaginal prolapse, using the Pelvic Organ Prolapse Quantification (POPQ) system. Methods: Cross-sectional study. The population consisted of patients who attended to the Hospital Universitario de Caracas with pelvic organ prolapse stage II or greater and who had involvement of the apical compartment between 2021 and 2022. The selection of the sample was made in a non-probabilistic, sequential way. Each patient underwent POPQ before and after simulation of apical support with the posterior blade of a standard Graves speculum. The Aa Ba Ap and Bp points were evaluated before and after simulation and the proportion of patients in whom the stage of anterior or posterior prolapse changed when simulating apical support was also determined. Results: We included 100 patients, most of them with prolapse stage III and IV. After apical support, the anterior compartment points were corrected to a value between 0 to -2 or -3 in most cases (p< 0.05). The points corresponding to the posterior vaginal wall changed to -3 in most patients (p< 0.05). The prolapse stage of the anterior compartment, changed to stage ≤ II in 99 % of cases (p< 0.05), and in 100% in the posterior compartment (p<0.05). Conclusion: Simulation of apical support during POPQ demonstrated correction of anterior and posterior compartment prolapse in a high percentage of patients(AU)
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Humans , Female , Middle Aged , Quality of Life , Cross-Sectional Studies , Pelvic Organ Prolapse , Patients , Obstetrics and Gynecology Department, Hospital , Uterine Prolapse , Simulation ExerciseABSTRACT
Background: In endemic regions of several countries, the prevalence of leprosy has not come down to the level of elimination. On the contrary, new cases are being detected in large numbers. Clinically, it is frequently noted that despite completion of multibacillary multidrug therapy for 12 months, the lesions remain active, especially in cases with high bacteriological indices. Aim: The present study focused on finding out the viable number of Mycobacterium leprae during the 12-month regimen of multibacillary multidrug therapy, at six and 12 months intervals and, attempting to determine their role in disease transmission. Methods: Seventy eight cases of multibacillary leprosy cases were recruited from leprosy patients registered at The Leprosy Mission hospitals at Shahdara (Delhi), Naini (Uttar Pradesh) and Champa (Chhattisgarh), respectively. Slit skin smears were collected from these patients which were transported to the laboratory for further processing. Ribonucleic acid was extracted by TRIzol method. Total Ribonucleic acid was used for real-time reverse transcription-polymerase chain reaction (two-step reactions). A standard sample with a known copy number was run along with unknown samples for a reverse transcription-polymerase chain reaction. Patients were further assessed for their clinical and molecular parameters during 6th month and 12th month of therapy. Results: All 78 new cases showed the presence of a viable load of bacilli at the time of recruitment, but we were able to follow up only on 36 of these patients for one year. Among these, using three different genes, 20/36 for esxA, 22/36 for hsp18 and 24/36 for 16S rRNA cases showed viability of M. leprae at the time of completion of 12 months of multidrug therapy treatment. All these positive patients were histopathologically active and had bacillary indexes ranging between 3+ and 4+. Patients with a high copy number of the Mycobacterium leprae gene, even after completion of treatment as per WHO recommended fixed-dose multidrug therapy, indicated the presence of live bacilli. Limitations: Follow up for one year was difficult, especially in Delhi because of the migratory nature of the population. Patients who defaulted for scheduled sampling were not included in the study. Conclusion: The presence of a viable load of bacilli even after completion of therapy may be one of the reasons for relapse and continued transmission of leprosy in the community
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Background: Postpartum hemorrhage (PPH), a complication encountered during third stage of labour, contributes to 25% of maternal death worldwide. Despite various measures for prevention and management of PPH, burden of PPH still looms.Methods: Prospective randomized controlled clinical trial (RCT) was conducted in Vydehi Institute of Medical Sciences, among 128 patients predisposed to PPH, over 18 months. After meeting the inclusion and exclusion criteria, participants were randomized to receive tranexamic acid (TXA) intravenously 10 mg/kg along with 10 IU of oxytocin following the delivery. Patients were analysed for, blood loss, need for medical or surgical interventions.Results: Parameters like age, mean gestational age at haemoglobin estimation, and at delivery were similar among groups. The need or parenteral iron, blood transfusions, uterine artery ligation and compression suture were higher in controls group, but not statistically significant. Among the cesarean section (CS) group, most significant pre-disposing factors for PPH were previous CS (p value=0.012) and anaemia (p value =0.01). Incidence of PPH 0.69% (p value =0.031) and use of additional uterotonics were statistically significant (p value <0.05). Among the vaginal delivery (VD) group, most significant pre-disposing factors were anaemia (p value =0.002), thrombocytopenia (p value =0.045), and fetal-macrosomia (p value =0.020). Incidence of PPH 0.25% (p value <0.001) and use of additional uterotonics and hospital stay were statistically significant.Conclusions: We conclude that, anemic patients were at higher risk of PPH irrespective of the mode of delivery. Prophylactic TXA lowers incidence of PPH, blood loss, use of additional uterotonics and hospital stay even in the presence of pre-disposing factors. Quantification of blood loss is better estimated by drop in haemoglobin after 24 hours.
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Background and objective: Phytochemicals are plant-based bioactive ingredients found in tea, fruits, and vegetables with multiple health benefits. This study aimed to identify and quantify the detectable phytochemicals in selected products of chamomile herbal tea (CHT) using the Gas Chromatography - Flame Ionization Detector (GC-FID) approach. Methods: Selected CHTs were coded as CHT-A to CHT-G. CHT-F was crude and unbranded however, CHT-G was an oil extract of CHT. Ethanolic extracts of CHT were analysed and quantified for their phytoconstituents using the GC-FID method. Results: Phytochemicals detected in their order of abundance in most CHTs were flavonoids > glycosides > alkaloids > steroids > anti-nutrients > saponins > tannins > resveratrol. The flavonoids comprised rutin > flavanone > flavone > anthocyanin > epicatechin > kaempferol > naringenin > proanthocyanin, etc. where the glycosides found in each CHT involved cardiac and cyanogenic glycosides. Alkaloids were highest in CHT-A as ribalinidine > spartein > lunamarin with ephedrine detected just in CHT-B, CHT-C, CHT-D, CHT-E and CHT-F. Saponins occurred in four samples mainly as sapogenin. Anti-nutrients were detected in each CHT and included the phytates and oxalates. Resveratrol was detected in three samples only. Conclusion: Most of the CHTs evaluated showed the presence of flavonoids, glycosides, alkaloids, steroids, anti-nutrients, saponins, tannins and resveratrol.
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Background: To assess the relief of symptoms and efficacy of vaginal hysterectomy and pelvic floor repair surgeries in patients with pelvic organ prolapse.Methods: A total of 55 women participated in the study. Vaginal hysterectomy with or without anterior and posterior colporrhaphy and fothergills surgery were done in all cases. Patients were followed postoperatively about symptom relief and the anatomical correction was analyzed at 6 months using Pelvic Organ Prolapse-Quantification (POP-Q) System.Results: The highest incidence of POP was between 41 to 50 years of age and in multiparous women with parity 2 and more than 4. 36.36% had stage III prolapse when measured using the POPQ classification. Something coming out per vaginum was the commonest complaint seen in 100% of the patients. The improvement of all symptoms was significant (p value <0.05).Conclusions: There is significant anatomical restoration and symptom relief after surgery by POPQ.
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Objective: The objective of the study was to develop and validate a new diffuse reflectance infrared fourier transform (DRIFT) spectroscopic method for the quantification of Tramadol HCl in the pharmaceutical dosage form.Methods: The Fourier Transform Infrared (FTIR) analysis was carried out on Cary 630 FTIR spectrophotometer (Agilent Technologies, USA) equipped with a diffuse reflectance sampling interface (650-4000 cm-1, 32 scans, 8 cm-1 resolution). The solid-state samples were prepared by dilution in dry potassium bromide and were analysed by an FTIR spectrophotometer with the DRIFT sampling technique, which follows the Kubelka-Munk model depicting the theory of diffuse reflectance at scattering surfaces, relating band intensities to concentration for transmission measurements similar to Beer’s law.Results: A linear relationship was found in the selected wavenumber range of 3295-3298 cm-1denoting the carboxyl peak in the concentration range of 0.6-3.0% w/w with a good correlation coefficient of 0.997. The percent recovery of Tramadol HCl in the marketed dosage form was found to be 100.4%.Conclusion: The proposed method was found to be accurate, precise, reproducible, and eco-friendly. DRIFT spectroscopy may have the potential as an alternative method for qualitative and quantitative analysis of Tramadol HCl in its capsules.
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Objective To design an ultra-fast thermal cycle fluorescent quantitative PCR system for on-site detection of pathogenic nucleic acids and evaluate its performances.Methods An ultra-fast thermal cycle fluorescent quantitative PCR system was developed with the components of a flat reaction cup,an ultra-fast thermal cycle module,a fluorescence detection module with fixed optical path and a data processing module based on the smartphone platform.The ultra-fast thermal cycle module was composed of a heating unit and a cooling unit,of which the heating unit was made of ceramic sheet and Ag/Pb alloy and the cooling unit consisted of a high-speed magnetic levitation cooling fan and a double-curved throat;the fluorescence detection module with fixed optical path was prepared with injection molding process,and made up of a light source excitation unit and a light detector unit;the data processing module based on the smartphone platform included a Bluetooth serial port adapter unit and a smartphone App,which used C2540F256 chip from TI company for developing the Bluetooth serial port adapter and Android Studio for the App.The ultra-fast thermal cycle fluorescent quantitative PCR system was used to detect influenza A/B virus and SARS-CoV-2 to verify its performances.Results The ultra-fast thermal cycle fluorescent quantitative PCR system realized rapid nucleic acid detection of influenza A/B virus and SARS-CoV-2,and the detection results were in high agreement with those by conventional real-time quantitative PCR.Conclusion The ultra-fast thermal cycle fluorescence quantitative PCR system gains advantages in small size and light weight,and can be used for rapid on-site detection of pathogen nucleic acids.[Chinese Medical Equipment Journal,2023,44(11):15-20]
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Objective:To explore the application of magnetic resonance(MR)quantitative analysis technique of fat in analyzing the relationships between liver fat fraction(LFF)and pancreatic fat fraction(PFF)and the severity of nonalcoholic fatty liver disease(NAFLD),and between them and liver function.Methods:A total of 185 patients who received MR whole-liver mDIXON sequence scan were selected,and their LFF and PFF were measured.They were divided into NAFLD group(160 cases)and control group(25 cases)according to the fat fraction(FF)of liver.The body mass index(BMI),alanine aminotransferase(ALT),triglycerides(TG),fasting blood glucose(FBG),low-density lipoprotein(LDL-c),high-density lipoprotein(HDL-c),LFF and PFF between two groups were compared.Spearman analysis method was adopted to analyze the correlation between LFF and various indicators,and between PFF and various indicators.Logistic regression analysis was used to analyze the influencing factors of NAFLD severity.Results:In 160 patients of NAFLD group,72 cases were mild degree(LFF WAS 5.1%-14.0%),and 76 cases were moderate degree(LFF was 14.1%-28.0%),and 12 cases were severe degree(LFF>28.0%).There were significant differences in LFF,PFF and BMI between NAFLD group and control group(tLFF=17.259,tPFF=9.058,tBMI=7.430,P<0.05),and the differences of ALT,TG,FBG,LDL-c and HDL-c between two groups also were significant(t=6.591,t=3.957,t=3.267,t=2.112,t=-3.727,P<0.05),respectively.There were significant differences in LFF,PFF,BMI,ALT,TG,FBG,LDL-c and HDL-c among mild group,moderate group and severe group of patients(F=21.944,F=16.391,F=5.872,F=30.240,F=3.984,F=3.863,F=3.398,F=1.214,P<0.05),respectively.BMI,ALT,TG and FBG appeared positive correlation with LFF and PFF of NAFLD patients(r=0.31,r=0.52,r=0.33,r=0.35,r=0.30,r=0.36,r=0.27,r=0.29,P<0.05),and HDL-c appeared negative correlation with them(r=-0.16,r=-0.3,P<0.05),respectively.Multivariate Logistic regression analysis showed that HDL-c was an independent protective factor of the severity of NAFLD(OR=0.004,P<0.05),and LFF,PFF,BMI,ALT,TG,LDL were independent risk factors of the severity of NAFLD(OR=2.252,OR=1.988,OR=1.404,OR=1.196,OR=1.025,OR=5.150,P<0.05),respectively.Conclusion:LFF and PFF are closely related to the severity of NAFLD and the indicators of liver function,and the increases of LFF and PFF are independent risk factors of the aggravation of NAFLD.The MR quantitative analyses of LFF and PFF values have a certain guiding significance in clinical monitoring for NAFLD severity and liver function.
ABSTRACT
Objective:To evaluate the systolic function of 24 segments in dichorionic diamniotic (DCDA) twin pregnancy by fetal heart qualification (fetal HQ) analysis, and to compare the difference with singleton pregnancies.Methods:Thirty-one cases of DCDA twin pregnancy (observation group) and 100 cases of singleton pregnancy (control group) matched in gestational age were enrolled. They underwent perinatal health care in the Third Affiliated Hospital of Zhengzhou University from February 2022 to February 2023.Fetal HQ was applied to track endocardial motion trajectories and automatically calculate the global strain (GS), fractional area change (FAC) and fractional shortening (FS) of 24 segments of left and right ventricles in order to compare the difference between DCDA fetuses and singleton fetuses. One case of twins with growth discordance were selected to demonstrate the utility of this technique.Results:①There were no statistically significant differences in GS, FAC and 24-segments FS of left and right ventricles between the two fetuses in the observation group (all P>0.05). ②The LV-FS of segments 1-10 were higher in the observation group than the control group, and the differences were statistically significant (all P<0.05). However, there were no statistically significant differences in GS and FAC of left and right ventricles, LV-FS of segments 11-24 and RV-FS of segments 1-24 between the observation group and the control group (all P>0.05). The intraclass correlation coefficient(ICC) was greater than 0.75, indicating good repeatability. ③Two fetuses with discordant growth in dichorionic diamniotic twins had abnormal FS in some segments of the left and right ventricles. Conclusions:Fetal HQ technology could easily and reliably evaluate fetal heart function in DCDA pregnancy with good repeatability. The changes of fetal cardiac function in twin pregnancy did not always follow the same pattern as in singleton pregnancy. The related data of singleton pregnancy should not be blindly used in the clinical management and scientific research of twin pregnancy.