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Chinese Journal of Biotechnology ; (12): 862-875, 2018.
Article in Chinese | WPRIM | ID: wpr-687730


Santalene and santalol are the main components of valuable perfume sandalwood essential oil, and have good antibacterial, anti-oxidation and anti-tumor activities. Commercial sandalwood essential oil is mainly extracted from sandalwood tree that grows slowly and is difficult to cultivate. In addition, the extraction recovery of sandalwood essential oil from sandalwood tree is too low to meet the market demand. These factors make sandalwood essential oil expensive. An option is to use genetic engineering and molecular biological methods to heterologously express related synthase of santalene and santalol in microbial host. In this paper, the biosynthesis progress of santalene and santalol synthase, as well as the optimization of mevalonate metabolic pathways in the hosts are summarized. Furthermore, the strategies of applying protein engineering technology to carry out orthomutation of santalene synthase were also discussed, to provide reference for the optimal biosynthesis of santalene and santalol.

Article in Chinese | WPRIM | ID: wpr-853519


Objective :To establish the GC fingerprint of Qishen Yiqi Dropping Pills(QYDP)and Dalbergiaodorifera oiland identify the common chromatographic peaks by GC-MS. Methods: The GC analysis was performed on an Agilent HP-INNOWaxcapillary gas chromatography column (30 m × 0.25 mm, 0.25 μm). The temperature program was as follows: 100 ℃ for 2 min, 160 ℃ at 3 ℃/min, 190 ℃ at 1 ℃/min, 220 ℃ at 5 ℃/min, then 250 ℃ at 10 ℃/min; Nitrogen was used as the carrier gas with its flow rate 0.5 mL/min; The sample injection temperature was 250 ℃ and the split ratio was 5:1; The detector was FID with its temperature of 300 ℃. MS parameters: MS electron energy of 70 eV, ion source temperature of 230 ℃, full-ion scan mass rangem/z 35-600, solvent delay for 8 min. Results: Thirty-three common peaks were found from QYDP and 25 common peaks were found from D.odorifera oil in their GC fingerprints. Twenty-three common peaks in the fingerprint ofD.odorifera oil,such as trans-nerolidol,3,7,11-trimethyl-3,6-epoxy-1,10-dodecadien-7-ol isomers, farnesol isomers, α-santalene, norbornane, andβ-bisabolene were identified. The similarity of all batches of QYDP and D.odorifera oil were both over 0.979. Conclusion: This method is simple and reliable and can be used to evaluate the quality of QYDP and D.odorifera oil.