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1.
Rev. colomb. biotecnol ; 23(2): 15-24, jul.-dic. 2021. tab, graf
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1360960

ABSTRACT

RESUMEN Varios virus con genoma de ARN en fases iniciales de la infección realizan la translocación de proteínas al interior del núcleo de la célula hospedera mediante la vía de las importinas α1. Este transporte es fundamental para el éxito de la replicación viral y se ha convertido en un blanco para la búsqueda y desarrollo de nuevos antivirales. El objetivo de este estudio fue determinar y caracterizar interacciones entre la Agatisflavona, Amentoflavona, Punicalina con el sitio mayor de unión de las Importinas α1 humanas mediante el análisis in silico del acoplamiento molecular y simulaciones de dinámica molecular. Las pruebas de acoplamiento molecular se realizaron entre estos fitoconstituyentes y la estructura de la importina α1 humana. Las afinidades de interacción fueron detectadas con la Agatisflavona, Amentoflavona y Punicalina (ΔG b = -8,8, -9,1 y -8,8 kcal.mol-1 respectivamente), con afinidades de interacción específicamente a los dominios ARM2-ARM5 (sitio mayor de unión) de las importinas α1. Las simulaciones de dinámica molecular revelaron interacciones significativamente favorables (P<0,001) con los ligandos Agatisflavona y Amentoflavona (ΔG b = -18,60±0,35 y -22,55±2,41 kcal.mol-1) mientras que la Punicalina registró mayores valores de energía de interacción (ΔG b = -5,33±1,72 kcal.mol-1). Los hallazgos obtenidos en este estudio computacional sugieren que las moléculas Agatisflavona y Amentoflavona presentan interacciones favorables con el sitio mayor de unión de las Importinas α1, en comparación a lo registrado con la Punicalina, sin embargo, se recomienda realizar ensayos in vitro a modo de confirmar estas observaciones.


ABSTRACT Several RNA-viruses during early stages of infection perform the translocation of proteins into the nucleus of host cell by the importin α1 pathway. This transport is essential for viral replication success and has become a target to search and development new antivirals. The objective of this study was to determine and characterize interactions between Agathisflavone, Amentoflavone and Punicalin with the major binding site of human importins α1 by in silico analysis of molecular docking and molecular dynamics simulations. Molecular docking tests were performed between these phytoconstituents and the structure of human importin α1. Interaction's affinity was detected with the Agathisflavone, Amentoflavone and Punicalin (ΔG b = -8.8, -9.1 and -8.8 kcal.mol-1 respectively), with binding affinity to ARM 2-ARM 5 domains (major binding site) of importins α1. Molecular dynamics simulations revealed significantly favorable interactions (P<0.001) with the ligands Agatisflavone and Amentoflavone (ΔG b = -18.60 ± 0.35 and -22.55 ± 2.41 kcal.mol-1) meanwhile Punicalin showed higher values of interaction free energy (ΔG b = -5.33 ± 1.72 kcal.mol-1). The findings obtained suggest that Agathisflavone and Amentoflavone could favorably interact to the major binding site of Importins α1 compared to that registered with Punicalin, however, it is recommended to perform in vitro assays to confirm these observations.

2.
Braz. j. oral sci ; 20: e214034, jan.-dez. 2021. ilus
Article in English | LILACS, BBO | ID: biblio-1282553

ABSTRACT

Aim: To compare Enterococcus faecalis reduction after antimicrobial photodynamic therapy (aPDT) used with methylene blue, toluidine blue, tannin, and curcumin as photosensitizers, an adjunct to endodontic chemomechanical preparation (CMP) in root canals of human teeth. Methods: A total of 120 single-rooted teeth were divided into 6 groups (n = 20): G1- CMP and 2.5% sodium hypochlorite (NaOCl); G2- CMP and saline solution; G3- CMP, 2.5% NaOCl, and aPDT with 0.005% methylene blue; G4- CMP, 2.5% NaOCl, and aPDT with 0.005% toluidine blue; G5- CMP, 2.5% NaOCl, and aPDT with 0.005% tannin; and G6- CMP, 2.5% NaOCl, and aPDT with 0.005% curcumin. A portable semiconductor laser was used (660 nm, 100 mW, 1.8 J, 180s) in groups 1, 2, 3, 4, 5, and a blue LED light-curing (420-480 nm, 1200 mV/cm2 ) in G6. For all groups, a 5 min pre-irradiation time was applied. Samples were collected before (initial collection), immediately after (intermediate collection) and 7 days after CMP (final collection) for colony-forming unit (CFU) counting. The Kruskal-Wallis test and analysis of variance (ANOVA) were performed (p < 0.05; 95% confidence interval). Results: In between-group comparisons, there was no significant difference observed in the number of CFUs at the initial (p >< 0.001) and final collections (p >< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies.>< 0.05; 95% confidence interval). Results: In between-group comparisons, there was no significant difference observed in the number of CFUs at the initial (p < 0.001) and final collections (p >< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies.>< 0.001) and final collections (p < 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies>< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies


Subject(s)
Photochemotherapy , Tannins , Photosensitizing Agents , Curcumin , Endodontics
3.
J. coloproctol. (Rio J., Impr.) ; 41(2): 138-144, June 2021. graf, ilus
Article in English | LILACS | ID: biblio-1286992

ABSTRACT

Abstract Due to the ethnopharmacological use of Campsiandra laurifolia (Fabaceae), popularly known as Acapurana, to treat wounds and ulcers, associated with the lack of alternative treatments for intestinal inflammations such as ulcerative colitis (UC), the present work sought to characterize its phytochemical and antioxidant activities, and to evaluate remedial action in experimental colitis with acetic acid. Phytochemical analyzes were performed through qualitative and quantitative colorimetric tests of the main secondary metabolites. In the colitismodel, 24male Wistar rats aged±60 days oldwere used, divided into 4 groups: Control (CO) control+aqueous extract of C. laurifolia 50mg/kg (CO+A50); Colitis (CL); and Colitis+aqueous extract of C. laurifolia 50 mg/kg (CL+ A50).Measurement of sphincter anal pressure and histological tests of the large intestine, lipoperoxidation (LPO), enzymeactivity of superoxide dismutase (SOD), and levels of glutathione (GSH)were performed. For statistical analysis, the oxidative stress (OS) results were expressed as means±standard error, adopting a significance level of p < 0.05. The screening indicated the presence of flavonoids, saponins and tannins in the extract, with high levels of phenolic


Resumo Devido ao uso etnofarmacológico de Campsiandra laurifolia (Fabaceae), popularmente conhecida comoAcapurana, para tratar feridas e úlceras, associado à falta dealternativas de tratamentos para as inflamações intestinais como a retocolite ulcerativa (RCU), o presente trabalho buscou caracterizar sua constituição fitoquímica, sua atividade antioxidante, e avaliar sua ação reparadora na colite experimental com ácido acético. As análises fitoquímicas foram realizadas por meio de ensaios colorimétricos qualitativos e quantitativos dos principaismetabólitos secundários.Nomodelo de colite, foramutilizados 24 ratos machos Wistar de±60 dias de idade, divididos em 4 grupos: Controle (CO), controle+ extrato aquoso de C. laurifolia 50mg/kg (CO+A50); Colite (CL); e Colite+extrato aquoso de C. laurifolia (CL+ A50). Foram realizadas aferições da pressão anal esfincteriana e avaliações histológicas do intestino grosso, lipoperoxidação (LPO), atividade da enzima superóxido dismutase (SOD) e níveis da glutationa (GSH). Para a análise estatística, resultados do estresse oxidativo (EO) foram expressos em médias±erro padrão, adotando um nível de significância de p < 0,05. O screening indicou no extrato a presença de flavonoides, saponinas e taninos com altos teores de compostos fenólicos e taninos, relacionando-os a uma elevada capacidade antioxidante. Na análise histológica, o grupo CL apresentou perda das criptas, do edema e do infiltrado inflamatório. O uso do extrato de C. laurifolia reestruturou as criptas, diminuiu o edema e aumentou a pressão anal esfincteriana, com diminuição da LPO, da SOD, e aumento da GSH. Sugere-se que o uso do extrato de C. laurifolia diminui o EO por seu poder antioxidante, conferido pelos compostos fenólicos presentes no extrato.


Subject(s)
Animals , Rats , Colitis/chemically induced , Antioxidants , Tannins , Oxidative Stress , Phenolic Compounds , Fabaceae
4.
Rev. MVZ Córdoba ; 26(1): 15-24, Jan.-Apr. 2021. tab, graf
Article in English | LILACS | ID: biblio-1351545

ABSTRACT

ABSTRACT Objective. This research aimed to evaluate the inclusion of Cucurbita foetidissima (BG) leaves as a partial or total substitution of alfalfa hay (AH) in beef cattle diets on in vitro methane output, gas kinetics and volatile fatty acids production. Materials and Methods. Five experimental treatments were formulated with the inclusion of BG as alfalfa hay (AH) substitute at 0, 25, 50, 75 and 100% in experimental treatments denominated CON (control), BG25, BG50, BG75 and BG100, respectively. Results. Lignin and organic matter decreased with BG inclusion (p<0.05). However, NDF, CT and TPC increased with higher levels of BG (p<0.05). Maximum gas production diminished with BG inclusion (p<0.05); whereas, microbial protein production, specific gas production rate and latency period were not affected (p>0.05). Methane production decreased linearly with BG inclusion (p<0.05). Nevertheless, CO2 production showed no changes with inclusion of graded levels of BG in the experimental treatments (p>0.05). Additionally, acetate and butyrate were not affected by BG inclusion (p>0.05); although, propionate increased linearly among treatments (p<0.05). Furthermore, inclusion of 75% of BG reduced 31% in vitro methane production without changes in CO2 production, suggesting an inhibition of endogenous methanogenesis. Conclusions. These results promote the inclusion of BG leaves as a potential and environmentally friendly forage source for beef cattle feeding.


RESUMEN Objetivo. El objetivo de este estudio fue evaluar la inclusión de hojas de Cucurbita foetidissima (BG) como sustituto parcial o total de la alfafa (AH) en la dieta de ganado de engorda sobre la cinética de producción de gas y producción ruminal in vitro de metano y ácidos grasos volátiles. Materiales y Métodos. Se formularon cinco dietas con la inclusión de BG como sustituto de heno de alfalfa a 0, 25, 50, 75 y 100% en los tratamientos experimentales que se denominaron CON (control), BG25, BG50, BG75 y BG100, respectivamente. Resultados. La lignina y la materia orgánica disminuyeron con la inclusión de BG (p<0.05). La máxima producción de gas disminuyó con la inclusión de BG (p<0.05); mientras que la producción de proteína microbiana, la tasa específica de producción de gas y el tiempo de latencia no mostraron cambios(p>0.05). La producción de metano disminuyó linealmente con la inclusión de BG (p<0.05). No obstante, la producción de CO2 no mostró cambios con la inclusión de BG en los tratamientos (p<0.05). Adicionalmente, el acetato y el butirato no fueron afectados por la inclusión de BG (p>0.05); a pesar de que el propionato se incrementó linealmente (p<0.05). Además, la inclusión de BG en un 75% redujo la producción de metano 31% sin afectar la producción de CO2, lo que sugiere una inhibición de la metanogenesis endógena. Conclusiones. Estos resultados promueven la inclusión de hojas de BG como una fuente de forraje potencial y amigable con el ambiente en la alimentación de ganado de engorda.


Subject(s)
Animals , Tannins , In Vitro Techniques , Cucurbita
5.
China Pharmacy ; (12): 933-939, 2021.
Article in Chinese | WPRIM | ID: wpr-876262

ABSTRACT

OBJECTIVE:To esta blish a method for si multaneous determination of 5 components in the branch and root of Juglans mandshurica as gallic acid ,ellagic acid ,1,6-di-O-galloyl-β-D-glucose,1,2,6-tri-O-galloyl-β-D-glucose and 1,2,3, 6-tetra-O-galloyl-β-D-glucose,and to analyze the content difference of above 5 components between the branch and root samples. METHODS:HPLC method was adopted. The determination was performed on Agilent Poroshell 120 SB-C18 column with mobile phase consisted of water (containing 0.2% formic acid )-acetonitrile (containing 0.2% formic acid ). A gradient elution was performed at a flow rate of 0.3 mL/min. The column temperature was 30 ℃ and the detection wavelength was 270 nm. The sample size was 5 μL. Independent samples t-test and partial least squares-discriminant analysis (PLS-DA)were applied for statistical analysis of 5 components. RESULTS :The linear range of gallic acid ,ellagic acid ,1,6-di-O-galloyl-β-D-glucose,1,2, 6-tri-O-galloyl-β-D-glucose and 1,2,3,6-tetra-O-galloyl-β-D-glucose were 0.989-63.3,1.58-101,1.01-64.7,3.31-212,3.34-214 μg/mL (r≥0.997 3),respectively. RSDs of precision ,reproducibility and stability tests (12 h)were all lower than 3.2%. The average recoveries of the 5 components were 103.2%(RSD=4.85%),99.1%(RSD=2.80%),101.5%(RSD=1.31%),102.9%(RSD= 2.73%)and 104.7%(RSD=1.28%),respectively. The average contents of the above components in the branch of J. mandshurica were 0.296 5,0.621 1,0.562 5,3.111 7 and 3.451 3 mg/g,respectively. The average contents of above components in the root were 0.673 4,2.755 5,0.964 0,2.946 6 and 4.836 4 mg/g,respectively. The total contents of the 5 components in the branch and roo t of J. mandshurica were 8.043 2 and 12.175 9 mg/g,respectively. The contents of gallic acid ,ellagic acid and 1,6-di-O-galloyl- β-D-glucose in roots were significantly higher than those in branches (P<0.05 or P<0.01). There were no significant differences in the contents of the other 2 components and the total contents of the 5 components in branches and roots (P>0.05). The cumulative interpretability (R 2X,R 2Y) and cumulative predictability (Q 2) of the model established by PLS-DA were 0.943,0.745,and 0.710 respectively. The model load diagram showed that the distance between the ellagic acid and the origin was the farthest ,and only variable projection importance of the content of the ellagic acid was greater than 1. CONCLUSIONS:The established method can be used for the content determination of 5 components in the branch and root of J. mandshurica . Except for 1,2,6-tri-O-galloyl-β-D-glucose,the contents of other 4 components and total contents of the 5 components in the root of J. mandshurica are higher than those of the branch. Ellagic acid is selected as the potential marker for discriminating the branch and root samples.

6.
Article in Chinese | WPRIM | ID: wpr-906183

ABSTRACT

Objective:To investigate the antioxidant activity and chemical composition of 75% ethanol extract of <italic>Rosa cymosa</italic> roots and its different polar parts. Method:The 75% ethanol extract of <italic>R. cymosa</italic> roots was divided into dichloromethane, ethyl acetate, <italic>n</italic>-butanol and water parts by organic solvent extraction. <italic>In vitro</italic> antioxidant activity of each fraction was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging assays, as well as ferric reducing antioxidant power (FRAP) test. The contents of total triterpenes, total phenols, total tannins and condensed tannins in each fraction were determined by spectrophotometry. SPSS 24.0 software was used to conduct Pearson correlation analysis between the antioxidant activity of each fraction and the content of the main components, and then the main active fraction and the main active components were determined. The chemical constituents of the active fraction was analyzed by ultra performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS), and the structures of the main chromatographic peaks were predicted. Result:Each fraction of <italic>R. cymosa</italic> roots had certain antioxidant activity, and there was a significant dose-effect relationship within a certain concentration range, but the antioxidant activity of different polar parts was different. In DPPH and ABTS free radical scavenging tests, the antioxidant activity of each fraction and vitamin C (VC, the positive drug) was ranked as ethyl acetate fraction>VC><italic>n</italic>-butanol fraction>ethanol extract>water fraction>dichloromethane fraction. In FRAP test, the activity of ethyl acetate fraction was weaker than that of VC, and the other order was unchanged. The contents of total triterpenes, total phenols, total tannins and condensed tannins in ethyl acetate fraction were 3.81%, 50.33%, 3.32%, and 39.79%, in <italic>n</italic>-butanol fraction were 0.88%, 41.42%, 2.25% and 23.55%, in ethanol extract were 2.90%, 41.95%, 3.43% and 20.14%, in water fraction were 0, 26.80%, 16.90% and 7.57%, and in dichloromethane fraction were 21.23%, 12.90%, 1.59%, and 6.17%, respectively. Correlation analysis results showed that the contents of total phenols and condensed tannins were positively correlated with the antioxidant activity, the contents of total triterpenes were negatively correlated with the antioxidant activity, and the correlation between total tannins and antioxidant activity was not obvious. A total of 26 compounds were identified from the ethyl acetate fraction by UPLC-Q-TOF-MS/MS, including 11 condensed tannins, 4 hydrolysable tannins, 6 triterpenes, 3 flavonoids, 1 benzoic acid derivative and 1 chlorogenic acid analogue. Conclusion:Ethyl acetate fraction is the main antioxidant active site of <italic>R. cymosa</italic> roots, and phenols mainly composed of condensed tannins are the main active components. The results can provide experimental basis for the development of natural antioxidants.

7.
Article in Chinese | WPRIM | ID: wpr-921794

ABSTRACT

In this paper, the newly isolated tannins were sorted after a review of the literature concerning tannins in recent 10 years, and their research progress was summarized in terms of extraction, isolation, pharmacological activity and metabolism. Hydrolysable tannins and condensed tannins are the main structural types. Modern research shows that tannins have many pharmacological effects, such as bacteriostasis, antioxidation, antitumor, antivirus and blood glucose reduction, and have broad development prospects. They are usually extracted by water, ethanol and acetone and isolated and purified by macroporous resin and gel column chromatography. The packings commonly adopted for the column chromatography mainly included Sephadex LH-20, Diaion HP-20, MCI-gel CHP-20 and Toyopearl HW-40. Modern analytical techniques such as nuclear magnetic resonance spectroscopy(NMR), fast atom bombardment mass spectrometry(FAB-MS) and circular dichroism(CD) are generally used for the structural identification of tannins. Howe-ver, their isolation, purification and structural identification are still challenging. It is necessary to use a variety of high-throughput screening methods to explore their pharmacological activities and to explore the material basis responsible for their functions through experiments in vivo.


Subject(s)
China , Hydrolyzable Tannins , Medicine, Chinese Traditional , Proanthocyanidins , Tannins
8.
Article | IMSEAR | ID: sea-203807

ABSTRACT

Areca catechu, commonly known as betel nut tree belongs to the family Arecacea. Itgrows in much of the tropical Pacific, Africa and parts of East Asia. It has some beneficial partslike husk, leaves, nuts and sheath which can be used for different purpose. It contains alkaloidsand tannins that have anthelmintic, antibacterial, antifungal, anti-inflammatory andantioxidant activities. The plant contains phytochemicals and phenols that have potentialbioactivity against diseases in humans. Traditional use of the plant for medicinal applicationshas to be documented for their possible use as future medicines or drugs. The nuts and leavesof the plant have also proved to be effective in the removal of heavy metals and inbioremediation. In this article, the medical applications of the areca plant as an herbal drugand its therapeutics application have been elaborated.

9.
Article | IMSEAR | ID: sea-210953

ABSTRACT

Present study was conducted in 240 day-old broiler chicks to assess the effect of condensed tannins (CT) containing guava leaf meal (GLM) supplemented diet on nutrient composition, litter metabolites viz. pH, moisture and ammonia concentration) and microbial counts after completion of feeding trial of 42 days. Chicks were randomly allocated into 4 dietary treatments, with 4 replicates of 15 chicks in each pen in a completely randomized block design (CRD). Chicks were fed basal diets supplemented with GLM @ 1.5, 2.5 and 3.5 percent, in T1, T2 and T3 groups, respectively, while, T0 group fed only basal diet. Litter materials of chicks showed good nutrient profiles and properly sterilized litter material act as alternative feed ingredient for ruminants feed. Supplementation of GLM in the diets of chicks significantly (P<0.05) reduced moisture, pH, NH3 concentration, harmful Eschericia coli and Clostridia spp. counts in the litter materials and reduced environmental pollution. The litter material of GLM supplemented (T1, T2 and T3) chicken showed significantly higher (P<0.05) CP contents as compared to un-supplemented control. It can be concluded that properly sterilized litter material of GLM supplemented chicks act as cheaper alternative feedstuffs for ruminant’s ration and reduce environmental pollution by lowering NH3 concentration, harmful E. coli and Clostridia counts in broiler litter material

10.
Rev. chil. nutr ; 47(2): 272-280, abr. 2020. tab, graf
Article in English | LILACS | ID: biblio-1115498

ABSTRACT

ABSTRACT The aim of this study was to develop and evaluate the antioxidant characteristics and sensory acceptance of Greek yogurt with added whole sorghum flours (WSF) from genotypes BRS305 (tannin) and BR501 (tannin-free). Five formulations were elaborated: 1) Control (without WSF); 2) 2% BR501; 3) 4% BR501; 4) 2% BRS305 and 5) 4% BRS305 and evaluated for antioxidant capacity, total anthocyanins, total phenols, condensed tannins, sensory acceptance and purchase intention. The 4% BRS305 formulation presented higher antioxidant properties, but lower sensory acceptance. No significant difference was observed for: color, flavor and overall acceptability between the control and 2% BR501; nor for texture and overall acceptability between the control and 4% BR501. Principal component analysis explained that all variables associated with antioxidant properties were positively correlated with the first major component (PC1: 82.7%). The 2% and 4% BR501 formulations were more highly correlated with PC1 (92.7%), as well as the control in relation to sensorial characteristics. The addition of WSF BRS305 improved the antioxidant properties of yogurts and the addition of WSF BR501 did not interfere with the sensory acceptance of the formulations. The similarity of the sensory acceptance of the yogurt containing WSF from the BR 501 genotype to the control, opens perspectives for the insertion of sorghum in human food, using dairy products as suitable matrices, adding potential functionality to this type of product.


RESUMEN El trabajo tuvo como propósito desarrollar y evaluar las características antioxidantes y la aceptación sensorial de yogurt griego con adición de harinas integrales de sorgo (HIS) de los genotipos BRS305 (con taninos) y BR501 (sin taninos). Cinco formulaciones fueron elaboradas: 1) Control (sin HIS); 2) 2% BR501; 3) 4% BR501; 4) 2% BRS305 y 5) 4% BRS305. Las formulaciones fueron evaluadas en función de su actividad antioxidante, antocianinas totales, fenólicos totales, taninos condensados, aceptación sensorial e intención de compra. La formulación 4% BRS305 presentó más propiedades antioxidantes, pero menor aceptación sensorial que las otras formulaciones. No se observó diferencia significativa entre el control y el 2% BR501 para el color, sabor e impresión global, ni tampoco para textura e impresión global en relación al 4% BR501. El análisis de componente principal ha explicado que todas las variables asociadas a las propiedades antioxidantes han sido positivamente correlacionadas con el primer componente principal (PC1: 82,7%). Las formulaciones 2% y 4% BR501 se han correlacionado más con el PC1 (92,7%), así como el control con las características sensoriales. La adición de HIS BRS305 ha mejorado las propiedades antioxidantes de los yogurts y la adición de HIS BR501 no ha interferido en la aceptación sensorial de las formulaciones. La similitud de la aceptación sensorial del yogurt que contiene el HIS del genotipo BR501 con el control, abre perspectivas para la inserción del sorgo en la alimentación humana, utilizando productos lácteos como matrices adecuadas, agregando funcionalidad potencial a este tipo de producto.


Subject(s)
Tannins/genetics , Yogurt , Sorghum/chemistry , Flour , Antioxidants/chemistry , Phenols/analysis , Tannins/analysis , Taste , Dairy Products , Principal Component Analysis , Functional Food , Anthocyanins/analysis
11.
Article in Chinese | WPRIM | ID: wpr-873231

ABSTRACT

Objective:To analyze the main chemical components in the supernatant and precipitate of Sanajon oral liquid, so as to provide basis for establishing its quality standard and precipitation control technology. Method:UPLC-Q-TOF-MSE was used to analyze the chemical components in the supernatant and precipitate of this oral liquid. The analysis was performed on Waters ACQUITY UPLC BEH C18 column (2.1 mm×100 mm, 1.7 μm) with the mobile phase of 0.1% formic acid solution (A) and acetonitrile (B) for gradient elution (0-1 min, 2%B; 1-2 min, 2%-5%B; 2-4 min, 5%-7%B; 4-6 min, 7%-24%B; 6-10 min, 24%-42%B; 10-12 min, 42%-54%B; 12-15 min, 54%-76%B; 15-18 min, 76%-100%B), the flow rate was set to 0.3 mL·min-1, the column temperature was 30 ℃, the injection volume was 2 µL. The mass spectrographic analysis was used with electrospray ionization (ESI), sample MS data was acquired by time-dependent MSE in negative ion mode, the collection range was m/z 50-1 200 (supernatant) and m/z 50-3 000 (precipitate). Then the chemical constituents were identified by the information of retention time, accurate relative molecular mass and secondary mass spectrum fragment. Result:Totally 61 compounds were identified in the supernatant, including tannins, phenolic acids, flavonoids, amino acids, organic acids, fatty acids, etc. Totally 15 compounds were identified in the precipitate, including tannins, phenolic acids, flavonoids, fatty acids, etc. Conclusion:The hydrolyzed tannin of Sanajon oral liquid may be the potential material basis of its precipitate, and its precipitate is likely to be a complex precipitate mainly composed of ellagic acid and tanned red. The established UPLC-Q-TOF-MSE can quickly and comprehensively analyze the chemical composition of Sanajon oral liquid, which can provide a scientific basis for the researches of its material basis, precipitation mechanism and quality control.

12.
Article in Chinese | WPRIM | ID: wpr-846588

ABSTRACT

Objective: To study the chemical constituents of tannin from the roots of Rheum palmatum. Methods: Chemical constituents of tannins were isolated by silica gel column chromatography, medium pressure column chromatography, and semi-preparative HPLC from the roots of R. palmatum. The structures were elucidated on the basis of spectral data analysis. Results: Four tannins, 3-O-cinnamoyl-1-O-galloyl-β-D-glucopyranoside (1), 2-O-cinnamoyl-1-O-galloyl-β-D-glucopyranoside (2), 2-O-cinnamoyl-1,6-di-O-galloyl-β-D-glucopyranoside (3), and 6-O-cinnamoyl-1-O-galloyl-β-D-glucopyranoside (4) were isolated from the roots of R. palmatum. Conclusion: Compound 1 is a new compound named palmatoside.

13.
Article in Chinese | WPRIM | ID: wpr-846424

ABSTRACT

Objective: The effects of different decoction time and different decoction temperature on its chemical constituents of anthraquinones, anthrone, and tannins were revealed by pseudo-targeted metabolomics, which provided the basis for clinical use of rhubarb. Methods: Using LTQ-Orbitrap-MSn to accurately excavate the characteristic ions of various chemical components of rhubarb, the ion pairs of chemical components were determined as much as possible by characteristic ions to obtain the peak area of various components by using the MRM mode of QQQ-MS. The effects of different decoction methods on the chemical components of rhubarb was compared by multivariate statistical analysis combined with paired t test. Results: Both decoction time and decoction temperature have impacts on the chemical components of rhubarb decoction. Short-term decocting for 15 min was beneficial to the dissolution of dianthrone glycosides and anthraquinone glycosides, while long-term decocting for 60 min was beneficial to the dissolution of tannins; Compared with boiling water maceration, boiling water decoction was more favorable for the dissolution of anthraquinones and tannins. Conclusion: This paper adopts the method of pseudo-targeted metabolomics, and clearly points out that short-term decocting or maceration is conducive to exerting the laxative effect of rhubarb, and long-time boiling decocting is conducive to exerting clearing heat and detoxifying efficacy, which provides a reference for the clinical application of rhubarb.

14.
Article in Chinese | WPRIM | ID: wpr-846422

ABSTRACT

Terminalia chebula is a traditional Chinese herbal medicine, which distributed in Yunnan, Guangdong, Guangxi, Tibet and etc. T. chebula is widely used in the clinical medicine of Chinese medicine and it plays a significant role in the Mongolian medicine and the Tibetan medicine. The chemical composition of T. chebula is rich and diverse, including phenolic acids, tannins, triterpenoids, aliphatics, flavonoids, volatile oils, amino acids, trace elements, carbohydrates and so on. Modern pharmacological studies have shown that T. chebula extract has many pharmacological activities, such as anti-oxidation, anti-cancer, anti-tumor, detoxification, antibacterial, strong heart, anti-inflammation, immunomodulation, anti-microbial, and promoting bronchial smooth muscle contraction. From the aspects of textual research, chemical composition characteristics, pharmacological action and so on, this paper expounds the research progress of T. chebula. According to the core concept of Q-marker, we predicted and analyzed the quality markers of T. chebula from the aspects of chemical composition characteristics, traditional efficacy, medicinal properties, pharmacokinetics, new clinical use and measurable composition. It provides reference for the quality evaluation of T. chebula.

15.
Article in Chinese | WPRIM | ID: wpr-846224

ABSTRACT

Objective: To study the chemical constituents of Changyanning Tablets, a high performance liquid chromatography-quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS/MS) was established to recognize and classify the ingredients accurately and rapidly. Methods: Agilent ZORBAX Eclipse XDB-C18 chromatographic column (250 mm × 4.6 mm, 5 μm) was employed and the separation was performed with the mobile phase consisting of methanol-0.05% acetic acid aqueous solution. The information of accurate mass and multistage fragment ions were obtained by the monitored simultaneously for positive and negative ions. The main chemical constituents of Changyanning Tablets were identified by high resolution mass spectrometry data, combining with Pubmed, Hmdb, Massbank network database, reference literature and comparing the reference. Results: Fifty-one chemical components were finally identified in this study, including two phenylpropanoids, eight iridoids, 12 flavonoids, four tannins, 23 organic acids, and two other classes. Conclusion: This study comprehensively studies the material basis in Changyanning Tablets, which provides a basis for improving the quality evaluation system of Changyanning Tablets and lays the foundation for elucidating the active components mechanism.

16.
Ciênc. rural (Online) ; 50(9): e20200095, 2020. tab
Article in English | LILACS-Express | LILACS | ID: biblio-1133325

ABSTRACT

ABSTRACT: The aim of this study was to evaluate the effect of the dietary inclusion of Acacia mearnsii tannin extract (TA) on nutrients intake and digestibility, and nitrogen (N) retention by sheep given a tropical grass-based diet. The trial was conducted with six castrated male sheep in a cross-over design in two 21-days experimental periods. The sheep were housed in metabolic cages and offered Tifton 85 hay (Cynodon dactylon) ad libitum plus concentrate at a rate of 12 g of dry matter (DM)/kg body weight (BW). The treatments were concentrate without (Control) or with 10 g TA/kg DM (Tannin). Concentration of TA in the diet was 3.8 g/kg DM and did not affect the feed intake or apparent digestibility. The TA decreased the true digestibility of n compounds (P<0.05) whereas did not impact the N retention, microbial N flow to the small intestine or the efficiency of rumen microbial protein synthesis. In conclusion, a low dietary concentration of TA did not impact the nutrients supply and N use by sheep fed with a tropical grass-based diet.


RESUMO: O objetivo do presente estudo foi avaliar o efeito da inclusão alimentar de extrato tanífero de Acacia mearnsii (TA) sobre o consumo, a digestibilidade e a retenção de nitrogênio (N) em ovinos alimentados com uma dieta a base de gramínea tropical. O experimento foi conduzido com seis ovinos machos castrados, em delineamento em reversão simples, com dois períodos experimentais de 21 dias cada. Os animais foram alojados em gaiolas metabólicas e alimentadas ad libitum com feno de Tifton 85 (Cynodon dactylon) mais concentrado oferecido a uma taxa de 12 g de matéria seca (MS)/kg de peso corporal. Os tratamentos foram: concentrado sem (Controle) ou com 10 g de TA/kg MS (Tanino). A concentração de TA na dieta foi de 3,8 g/kg MS e não afetou o consumo e nem a digestibilidade aparente dos nutrientes. O TA diminuiu a digestibilidade verdadeira do N (P<0,05), mas não afetou a retenção de N, o fluxo microbiano de N para o intestino delgado ou a eficiência de síntese de proteína microbiana no rúmen. Em conclusão, a inclusão de uma baixa dose de TA/kg MS na dieta não afetou a oferta de nutrientes nem o uso de N em ovinos alimentados com uma dieta baseada em uma gramínea tropical.

17.
Article | IMSEAR | ID: sea-203636

ABSTRACT

Background and Objective: Tapak Liman leaves have been long used traditionally for anemia. The dried powder form ofElephantropus scaber was extracted to produce ethanol. This study aimed to prove the hematopoietic effect of tapak limanleaf. Materials and Methods: The study was conducted for 28 days. The samples consisted of one non-anemic group andfour anemic groups induced by 130 mg/kgBW chloramphenicol given every day for 14 days. In the last day, group 2, 3 and 4were given the extract of tapak liman leaves for 10, 30 and 100 mg/kgBW; respectively. Group 5 (as a positive control) wasgiven physiological NaCl 0.9%. The testing doses were given once a day for 14 days, and the effects were observed on day1st , 14th, 21st, and 28th. Results: The parameters used in this study were erythrocyte, hemoglobin, reticulocyte andhematocrit counts in white mice that were analyzed by two-way ANOVA showing a significant value of (p<0.05).Conclusions: The extract of tapak liman leaves could increase hematopoietic activities; hence, it could be used as analternative therapy for anemia.

18.
Article in Chinese | WPRIM | ID: wpr-798501

ABSTRACT

Objective:To study on the chemical constituents of total tannins from Spatholobi Caulis,and to analyze the pharmacodynamics and mechanism of total tannins from Spatholobi Caulis on cervical cancer HeLa cells. Method:UPLC-Q-TOF/MS was used to qualitatively analyze the composition of total tannins from Spatholobi Caulis.The appropriate concentration and time of administration were screened by 3 dimensional(3D) microfluidic chip.Flow cytometry was used to determine the effect of total tannins from Spatholobi Caulis on the cell cycle and apoptosis of cervical cancer HeLa cells and analyzed by FlowJo v10.0.7 and ModFit LT 3.2 software.Enzyme-linked immunosorbent assay(ELISA) was used to determine the changes of vascular endothelial growth factor(VEGF)-A and Caspase-3 factors in cervical cancer HeLa cells supernatant treated with total tannins from Spatholobi Caulis. Result:Total of 15 components in total tannins from Spatholobi Caulis were identified or inferred.The low,medium and high dosages of total tannins from Spatholobi Caulis were 0.5,1.0, 2.0 g·L-1 and the best time of administration was 36 h.The proportions of early and late apoptosis of cervical cancer HeLa cells increased significantly in the apoptosis analysis after being treated by total tannins from Spatholobi Caulis.The DNA synthesis early phase(G0/G1 phase) of cervical cancer HeLa cells significantly increased,and the DNA synthesis phase(S phase) and the DNA synthesis late phase(G2/M phase) reduced.After being treated with total tannins from Spatholobi Caulis,the expression of VEGF-A in cervical cancer HeLa cells supernatant was significantly decreased and the expression of Caspase-3 was significantly increased. Conclusion:Spatholobi Caulis is rich in tannins,which can significantly inhibit the proliferation of cervical cancer HeLa cells and promote its apoptosis.This paper can provide the basis for further research of total tannins from Spatholobi Caulis.

19.
Article in Chinese | WPRIM | ID: wpr-802210

ABSTRACT

Objective:To elucidate the characteristics of bidirectional regulation of raw and steamed products of Rhei Radix et Rhizoma and their active components, in order to provide scientific basis for clinical rational use of this decoction pieces. Method:Mice were randomly divided into the blank group(distilled water, 10 mL·kg-1), raw rhubarb group(1.62 g·kg-1), steamed rhubarb group(0.972 g·kg-1), raw rhubarb anthraquinone group(0.22 g·kg-1), steamed rhubarb anthraquinone group(0.19 g·kg-1), raw rhubarb tannin group(0.17 g·kg-1) and steamed rhubarb tannin group(0.027 g·kg-1). Each group was divided into 3 batches of 10 mice. Mice in each group were given the corresponding dose by continuous gavage for 7 days, the diarrhea index(EI) of mice in each group was recorded every day. The serum levels of motilin(MTL), vasoactive intestinal peptide(VIP) and epinephrine(EPI) were measured on day 1,3,7. Result:Compared with the blank group, EI of the rhubarb group increased significantly on the 3rd day(Pth day of administration(Prd day(Pth day(Pst day of administration, on the 3rd day of administration, the MTL level in the steamed rhubarb anthraquinone group increased significantly(PPPth day of administration, the MTL levels of the raw and steamed rhubarb tannin groups increased to the level in the blank group, the VIP level of the raw rhubarb anthraquinone group increased significantly(PPConclusion:The combined anthraquinone and hydrolyzable tannin in rhubarb can promote gastrointestinal motility and diarrhea. The effect of astringent intestines is caused by the monomer tannin produced by digestion and decomposition of condensed tannin, which may be one of the mechanisms of bidirectional regulation of rhubarb decoction pieces.

20.
Article in Chinese | WPRIM | ID: wpr-851186

ABSTRACT

Objective: To analyse the fouling mechanism of tannins simulated solution membrane process. Methods: Taken the simulated solution consisting of tannins and small molecularly effective substances (berberine and geniposide) as an object of this study, the flux, the removal rate of the macromolecular substances, the size distribution of the different simulated solutions, and the was investigate by Hermia model in this study. Results: For tannins simulated solution and tannins + berberine + gardenoside simulated solution, fouling occured within only one phase controlled by cake formation. Due to the presence of the cake layer, the permeation rate of berberine and geniposide was not ideal in the simulated solution (67.63% and 87.55%, respectively). Conclusion: The Hermia mathematical model could be used as an important method to study the mechanism of the membrane fouling of Chinese herbal extracts. The formation and presence of the filter cake layer may be one of the reasons for the serious pollution of the traditional Chinese medicines’ membrane process.

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