ABSTRACT
Objective:To investigate the chemical constituents from sprout of Ziziphi jujuba var. spinosa,and explore the effect of germination on the activity of Z. jujuba var. spinosa seed from the perspective of material basis. Method:The dry sprout of Z. jujuba var. spinosa was crushed and extracted with petroleum ether to remove oils and fats,then the residues were dried and received reflux extraction with methanol. The solvent was decompressed and recovered to obtain methanol fractions. The fractions were dissolved, isolated and purified by column chromatography technologies such as silica gel,RP C18 and Sephadex LH-20,and the structures of the compounds were identified by nuclear magnetic resonance (NMR),mass spectrometry (MS) and reference liteature. Result:The 13 compounds were isolated from the sprout of Z. jujuba var. spinosa and identified as jujuboside B (1),jujuboside D (2),jujuboside A (3),betulinic acid (4),betulin (5),lupeol (6),alphitolic acid (7),ceanothic acid (8),oleanolic acid (9),ursolic acid (10),magnoflorine (11),nuciferine (12) and swertisin (13). Conclusion:All the above compounds were isolated from the sprout of of Z. jujuba var. spinosa for the first time. The study showed that the chemical constituents from the sprout were quite similar with those from the seeds of Z. jujuba var. spinosa,mainly including triterpenoids and saponins,flavonoids,alkaloid,etc.Overall,the results described in this study could provide certain chemical basis for further resources exploitation and utilization of the sprout of Z. jujuba var. spinosa.