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1.
Braz. j. biol ; 84: e253616, 2024. tab, graf
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1355880

ABSTRACT

Abstract This study evaluated the effect of the volatile oil of Alpinia zerumbet (VOAz) on caveolin-1 gene expression and muscular fibrosis. The rats were immobilized to induce fibrosis of the gastrocnemius muscle, and they were treated with VOAz. Collagen quality was assessed by histology and the expression of the caveolin-1 (CAV-1) gene was evaluated using qPCR. Histomorphological analysis indicated a significant reduction in the perimeter, width, and intensity of collagen in the treated groups, thus showing that the oil was effective in regulating the quality of collagen at the three concentrations. The results of expression levels suggested a decrease in the lesioned group and in two treatment groups (0.0115 µg/g and 0.009 µg/g). However, with the lowest concentration (0.0065 µg/g), no significant difference was observed, with levels similar to those found in healthy tissue. Therefore, the results showed that VOAz has the potential to be a non-invasive and low-cost alternative to aid in the treatment of muscular fibrosis.


Resumo Este estudo avaliou o efeito do óleo volátil de Alpinia zerumbet (OVAz) na expressão do gene da caveolina-1 e na fibrose muscular. Os ratos foram imobilizados para induzir a fibrose do músculo gastrocnêmio, e foram tratados com OVAz. A qualidade do colágeno foi avaliada com histologia e à expressão do gene caveolina-1 (CAV-1) foi avaliada usando qPCR. A análise histomorfológica indicou uma redução significativa no perímetro, largura e intensidade do colágeno nos grupos tratados. Os resultados dos níveis de expressão sugeriram diminuição nos grupos de lesão e em dois grupos de tratamento (0,0115 µg/g e 0,009 µg/g). No entanto, com a menor concentração (0,0065 µg/g), não foi observada diferença significativa, apresentando níveis semelhantes aos encontrados em tecido saudável. O uso do OVAz foi eficaz para reverter as alterações do colágeno causadas pela fibrose, e sua menor concentração apresentou uma possível tendência de aumento na expressão do CAV-1. Portanto, os resultados mostraram que o OVAz tem potencial para ser uma alternativa não invasiva e de baixo custo para auxiliar no tratamento da fibrose muscular.


Subject(s)
Animals , Rats , Oils, Volatile/pharmacology , Collagen/metabolism , Alpinia/chemistry , Caveolin 1/metabolism , Muscles/drug effects , Fibrosis , Plant Oils/pharmacology , Brazil , Rats, Wistar , Disease Models, Animal , Muscles/pathology
2.
China Pharmacy ; (12): 378-384, 2022.
Article in Chinese | WPRIM | ID: wpr-913099

ABSTRACT

The volatile oil of Magnolia liliflora ,as the main active part of the medicinal value ,has rich chemical constituents , mainly including cineole ,farnesol,α-pinene,β-pinene,terpineol,cadinene,linalool,citronellol,camphor. It has a wide range of pharmacological effects ,mainly anti-inflammatory and anti-allergic effects. Clinically ,it is mainly used to treat acute and chronic rhinitis,allergic rhinitis and sinusitis. This paper summarizes the research reports on the volatile oil of M. liliflora at home and abroad in the past 10 years,compares the chemical constituents of the volatile oil from M. liliflora under different conditions (origin,variety,part and extraction method ),combs its pharmacological effects and clinical application status ,and looks forward to its development prospect ,in order to provide reference for the further development of the medicinal value of the volatile oil from M. liliflora .

3.
Braz. j. biol ; 81(4): 881-886, Oct.-Dec. 2021. tab, graf
Article in English | LILACS | ID: biblio-1153454

ABSTRACT

Abstract Regarding the proven anticonvulsant effect of Zhumeria majdae essential oil (ZMEO) in previous studies we were prompted to investigate the ZMEO effects on the tolerance to the anticonvulsant effects of morphine and the morphine withdrawal syndrome. Tolerance to the morphine anticonvulsant effect was induced in mice by subcutaneous injection of 2.5 mg/kg of morphine for 4 days. Subsequent doses of ZMEO (20 mg/kg) were used to study the expression and development of morphine tolerance. Clonidine was used as the standard drug to inhibit the morphine withdrawal syndrome symptoms. To study the ZMEO effect on withdrawal syndrome, mice received appropriate morphine values for 4 days and on the fifth day, 60 min before administration of naloxone. The effective dose of ZMEO was determined and the number of jumps, stands and changes in the dry stool weight, as symptoms of withdrawal syndrome were evaluated. The dose of 20 mg/kg of ZMEO decreased the tolerance in development and expression groups significantly. Counting the number of jumping, standing and defecation were assessed 30 min after morphine and 1 h after the vehicle and clonidine. The dose of 40 mg/kg ZMEO decreased all the signs of withdrawal syndrome significantly. ZMEO was analyzed by GC/MS and linalool (53.1%) and camphor (23.8%) were characterized as the main components. The results suggest that ZMEO possesses constituent(s) that have activity against tolerance to the anticonvulsant effects of morphine and the morphine withdrawal symptoms.


Resumo Em relação ao efeito anticonvulsivante comprovado do óleo essencial de Zhumeria majdae (ZMEO) em estudos anteriores, fomos instigados a investigar os efeitos do ZMEO em relação à tolerância aos efeitos anticonvulsivantes da morfina e da síndrome de abstinência de morfina. A tolerância ao efeito anticonvulsivante da morfina foi induzida em camundongos por injeção subcutânea de 2,5 mg/kg de morfina por 4 dias. Doses subsequentes de ZMEO (20 mg/kg) foram utilizadas para estudar a expressão e o desenvolvimento da tolerância à morfina. A clonidina foi usada como droga padrão para inibir os sintomas da síndrome de abstinência da morfina. Para estudar o efeito do ZMEO na síndrome de abstinência, os camundongos receberam valores apropriados de morfina por 4 dias e, no 5º dia, 60 minutos antes da administração de naloxona. A dose efetiva de ZMEO foi determinada, e o número de saltos e de permanência e as alterações no peso das fezes secas, conforme os sintomas da síndrome de abstinência, foram avaliados. A dose de 20 mg/kg de ZMEO diminuiu significativamente a tolerância nos grupos de desenvolvimento e expressão. A contagem do número de saltos, permanência e defecação foi avaliada 30 minutos após a morfina e 60 minutos após o veículo e a clonidina. A dose de 40 mg/kg de ZMEO diminuiu significativamente todos os sinais da síndrome de abstinência. O ZMEO foi analisado por GC/MS, e linalol (53,1%) e cânfora (23,8%) foram caracterizados como os principais componentes. Os resultados sugerem que o ZMEO apresenta constituintes que possuem atividade contra a tolerância aos efeitos anticonvulsivantes da morfina e aos sintomas de abstinência da morfina.


Subject(s)
Animals , Rabbits , Substance Withdrawal Syndrome/drug therapy , Oils, Volatile , Pentylenetetrazole/toxicity , Pentylenetetrazole/therapeutic use , Seizures/chemically induced , Seizures/drug therapy , Morphine/therapeutic use , Anticonvulsants/therapeutic use
4.
Bol. latinoam. Caribe plantas med. aromát ; 20(3): 260-269, may. 2021. tab
Article in English | LILACS | ID: biblio-1342820

ABSTRACT

The aim of this study was to undertake a screening experiment on essential oils (EO) of Myrcia lundiana, Ocimum basilicum and Lippia alba against six food-spoiling pathogenic bacteria. Seventy-two (72) samples were initially analyzed fo antimicrobial activity based on the agar diffusion test. The minimum inhibitory (MIC) and bactericidal (MBC) concentrations were determined for the 12 samples which showed greatest antimicrobial potential in this stage. Two samples of L. alba, three samples of M. lundiana and seven samples of O. basilicum showed a MIC of 0.12-125 µL/mL for the six tested bacteria. Of these, the EO of O. basilicum cultivar Maria Bonita stood out with the lowest MIC and MBC. Thus, a mixture simulating this essential oil was prepared from commercial standards of the compounds (±)-linalool, geraniol and 1,8-cineole. Significantly higher MIC and MBC were detected in the simulation compared to the respective EO, suggesting a synergistic effect between compounds.


El objetivo de este estudio fue realizar un experimento de detección en aceites esenciales (AE) de Myrcia lundiana, Ocimum basilicum y Lippia alba contra seis bacterias patógenas que estropean los alimentos. Setenta y dos (72) muestras fueron analizadas inicialmente para la actividad antimicrobiana basada en la prueba de difusión en agar. Se determinaron las concentraciones mínimas inhibitoria (CMI) y bactericida (CMB) para las 12 muestras que mostraron el mayor potencial antimicrobiano en esta etapa. Dos muestras de L. alba, tres muestras de M. lundiana y siete muestras de O. basilicum mostraron un CMI de 0.12-125 µL/mL para las seis bacterias analizadas. De estos, el AE de O. basilicum cultivar Maria Bonita se destacó con el CMI y CMB más bajos. Por lo tanto, se preparó una mezcla que simula este aceite esencial a partir de los estándares comerciales de los compuestos de (±)-linalol, geraniol y 1,8-cineol. Se detectaron CMI y CMB significativamente más altos en la simulación en comparación con el AE respectivo, lo que sugiere un efecto sinérgico entre los compuestos.


Subject(s)
Bacteria/drug effects , Oils, Volatile/pharmacology , Ocimum basilicum/chemistry , Myrtaceae/chemistry , Lippia/chemistry , Anti-Bacterial Agents/pharmacology , Sesquiterpenes/analysis , Oils, Volatile/chemistry , Microbial Sensitivity Tests , Food , Anti-Bacterial Agents/chemistry
5.
Article in Chinese | WPRIM | ID: wpr-888103

ABSTRACT

Volatile oil is the main effective component and an important quality indicator of Artemisia argyi leaves. In this study, 100 germplasm resources of A. argyi were collected from all the related habitats in China. The total volatile oils in A. argyi leaves were extracted by steam distillation and the content was determined by GC-MS. The result demonstrated that the content of total volatile oils was in the range of 0.53%-2.55%, with the average of 1.43%. A total of 39 chemical constituents were identified from the volatile oils, including 13 shared by the 100 germplasm resources. Clustering analysis of the 39 constituents showed that the 100 A. argyi samples were categorized into groups Ⅰ(9), Ⅱ(2), Ⅲ(66) and Ⅳ(23), and group Ⅲ had the most volatile medicinal components, with the highest content. Five principal components(PCs) were extracted from 13 shared constituents, which explained 73.454% of the total variance. PC1, PC2, and PC3 mainly reflected the pharmacological activity of volatile oils and the rest two the aroma information. The volatile oils identified in this study lay a foundation for variety breeding of and rational utilization of volatile oils in A. argyi leaves.


Subject(s)
Artemisia , Distillation , Oils, Volatile , Plant Breeding , Plant Leaves
6.
Article in Chinese | WPRIM | ID: wpr-888043

ABSTRACT

To study the material basis and mechanism of volatile oil from Alpinia oxyphylla in treating Alzheimer's disease(AD) based on GC-MS and network pharmacology. Ingredients of volatile oil from A.oxyphylla were analyzed by GC-MS. Targets of those ingredients were obtained through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP). Relevant targets of AD were obtained through such databases as DrugBank, STITCH, OMIM. Intersection targets of ingredients and diseases were obtained by Online Venny map, and PPI network was established by STRING to screen out core targets. Gene ontology(GO) functional enrichment analysis and KEGG pathway enrichment analysis were performed by DAVID. The "ingredients-target-pathway" network was constructed by software Cytoscape 3.8.1 to screen out potential active ingredients of volatile oil from A.oxyphylla in the treatment of AD. The results showed that a total of 6 active ingredients were screened from the volatile oil of A.oxyphylla by GC-MS, 17 targets corresponding to 6 active ingredients were found in TCMSP database, and 3 448 AD targets were found in DrugBank database. "Ingredients-target-pathway" network and PPI network showed there were 4 potential active ingredients in the treatment of AD and 4 core targets. GO analysis and KEGG analysis showed 34(P<0.05) and 5(P<0.05) pathways, respectively, including nerve ligand receptor interaction, calcium signaling pathway, cholinergic synapse and 5-hydroxytryptaminergic synapse. This suggested that volatile oil from A.oxyphylla could synergistically treat AD by regulating calcium balance, cholinergic balance and phosphorylation. This study provided reference and guidance for further study of volatile oil from A.oxyphylla in the treatment of AD.


Subject(s)
Alpinia , Alzheimer Disease/genetics , Drugs, Chinese Herbal , Gas Chromatography-Mass Spectrometry , Humans , Molecular Docking Simulation , Oils, Volatile
7.
Article in Chinese | WPRIM | ID: wpr-888014

ABSTRACT

The volatile oil of Curcumae Rhizoma has many active components,which are the key to the quality of Curcumae Rhizoma. Exploring the difference between volatile oil of different kinds of Curcumae Rhizoma facilitates the quality control and rational application of resources. In this study,GC-MS was applied to realize online qualitative and semi-quantitative analysis of the chemical composition spectrum of volatile oil from Curcuma wenyujin( CW),C. phaeocaulis( CP),and C. kwangsiensis( CK). Forty components were identified and their fingerprints were compared and evaluated. Hierarchical cluster analysis( HCA),principal component analysis( PCA),and orthogonal partial least squares discrimination analysis( OPLS-DA) were adopted to analyze the overall and outlier data. The results showed that the whole data could be divided into three kinds according to each analysis mode,and the volatile components of Curcumae Rhizoma vary greatly among species. PCA explored the difference between outliers and the mean value of the group and found that some volatile oils from CW may be greatly affected by the origin. By OPLS-DA,the samples from Zhejiang were able to gather,but those from Guizhou remained isolated,indicating the influence of growing environment on Curcumae Rhizoma metabolites. Based on VIP results combined with the heat map,characteristic volatile oil components of Curcumae Rhizoma from different varieties were screened out: curdione and linalool for CW; 2-undecanone for CP; humulene,γ-selinene,and zederone for CK. The GCMS method established in this study describes Curcumae Rhizoma samples comprehensively and accurately,and the characteristic components screened based on chemometrics can be used to distinguish Curcumae Rhizoma from different varieties and give them unique pharmacodynamic significance,which is fast,convenient,stable,and reliable and supports the rational application of Curcu-mae Rhizoma resources. It is found that the region of origin has great influence on CW,which is worthy of further study.


Subject(s)
Curcuma , Gas Chromatography-Mass Spectrometry , Oils, Volatile , Principal Component Analysis , Rhizome
8.
Article in Chinese | WPRIM | ID: wpr-888010

ABSTRACT

This study explores the emulsifying material basis of Angelicae Sinensis Radix volatile oil (ASRVO) based on partial least squares (PLS) method and hydrophile-lipophile balance (HLB) value.The turbidity of ASRVO emulsion samples from Gansu,Yunnan,and Qinghai was determined and the chemical components in the emulsion were analyzed by GC-MS.The PLS model was established with the chemical components as the independent variable and the turbidity as the dependent variable and evaluated with indexes R~2X and R~2Y.The chemical components which were in positive correlation with the turbidity were selected and the HLB values were calculated to determine the emulsification material basis of ASRVO.The PLS models for the 81 emulsion samples had high R~2X and R~2Y values,which showed good fitting ability.Seven chemical components,2-methoxy-4-vinylphenol,trans-ligustilide,3-butylidene-1(3H)-isobenzofuranone,dodecane,1-methyl-4-(1-methylethylidene)-cyclohexene,trans-beta-ocimene,and decane,had positive correlation with turbidity.Particularly,the HLB value of 2-methoxy-4-vinylphenol was 4.4,which was the HLB range of surfactants to be emulsifiers and 2-methoxy-4-vinylphenol was positively correlated with turbidity of the ASRVO emulsion samples from the main producing area.Therefore,2-methoxy-4-vinylphenol was the emulsifying material basis of ASRVO.The selected emulsifying substances can lay a foundation for exploring the emulsification mechanism and demulsification solution of ASRVO.


Subject(s)
China , Emulsions , Least-Squares Analysis , Oils, Volatile , Surface-Active Agents
9.
Article in Chinese | WPRIM | ID: wpr-906029

ABSTRACT

Objective:To explore the effect of different proportions of organic fertilizer and chemical fertilizer on the agronomic traits, yield and quality of Artemisiae Argyi Folium in Qichun county, and provide a theoretical basis for scientific fertilization of its planting. Method:Field plot experiment was carried out to set up 5 treatment methods with different proportions of organic fertilizers and chemical fertilizers[OM<sub>0</sub> (no combined application of biological organic fertilizer), OM<sub>17</sub> (combined application of 17% biological organic fertilizer), OM<sub>33</sub> (combined application of 33% biological organic fertilizer), OM<sub>67</sub> (combined application of 67% biological organic fertilizer), OM<sub>100</sub> (combined application of 100% biological organic fertilizer)]. The effects of different treatment methods on the agronomic characters, leaf yield, output rate of moxa, volatile oil content, flavonoid and phenolic acid contents and mineral element contents of Artemisiae Argyi Folium in Qichun county were determined. Result:With the increase of the proportion of organic fertilizer in application, the seedling number per unit area, plant height, stem diameter, leaf number, leaf width, leaf length, height of dead leaves and leaf yield of Artemisiae Argyi Folium were increased at first and then decreased. Among them, the yield of Artemisiae Argyi Folium in OM<sub>33</sub> treatment was 61.37% higher than that in OM<sub>0</sub> treatment. With the increase of the proportion of organic fertilizer, the output rate of moxa of Artemisiae Argyi Folium showed continuously increasing trend, contents of volatile oil and volatile components (eucalyptol, <italic>α</italic>-thujone, borneol, camphor and caryophyllene oxide) increased at first and then decreased, while the contents of <italic>α</italic>-caryophyllene and <italic>β</italic>-syringene decreased gradually, the contents of phenolic acids (neochlorogenic acid, chlorogenic acid, isochlorogenic acid B, A and C) increased at first and then decreased, while the contents of flavonoids (jaceosidin and eupatilin) increased continuously, and the contents of mineral elements (Ca, Cu and Zn) continued to increase, but the content of K decreased significantly at the high proportion of organic fertilizer. After treated with principal component analysis (PCA), it was found that OM<sub>17</sub> treatment had the highest quality, while OM<sub>100</sub> and OM<sub>0</sub> treatment had low quality. Conclusion:Based on comprehensive analysis of agronomic traits, yield and quality indexes of Artemisiae Argyi Folium in Qichun county, it is suggested that 17%-33% proportion of organic fertilizer should be used in its production, in order to improve the quality and efficiency of Artemisiae Argyi Folium industry in Qichun county.

10.
Article in Chinese | WPRIM | ID: wpr-905858

ABSTRACT

Objective:To explore the underlying mechanism of volatile oil from Sishenwan in treating chronic ulcerative colitis through the Toll-like receptor (TLR)/myeloid differentiation factor 88 (MyD88) signaling pathway. Method:The BALB/c mice were randomly divided into a normal group (normal), a model group [dextran sodium sulfate (DSS)], a Sishenwan volatile oil group, an Ershen pill volatile oil group, a Wuweizi powder volatile oil group, and a mesalazine control group. The chronic ulcerative colitis model was induced by DSS in mice. Seven days after intragastric administration, the efficacy was evaluated based on the body weight, colon weight, colon weight index, colon length, and pathological damage score under colonoscopy. The levels of interleukin (IL)-4, IL-10, IL-17A, IL-21, and interferon-<italic>γ </italic>(IFN-<italic>γ</italic>) in the supernatant of colon tissues were detected by enzyme-linked immunosorbent assay (ELISA). Western blot was used to detect the expression levels of proteins related to the TLR/MyD88 signaling pathway in the colon mucosa of mice, including TLR2, MyD88, Ras-related C3 botulinum toxin substrate 1 (Rac1), IL-1 receptor-associated kinase 4 (IRAK4), IRAK1, tumor necrosis factor receptor (TNFR)-associated factor 6 (TRAF6), transforming growth factor-<italic>β</italic>-activated kinase 1 binding protein 1 (TAB1), TAB2, mitogen-activated protein kinase kinase 6 (MKK6), p38 mitogen-activated protein kinase (p38 MAPK), and cyclic adenosine monophosphate response element-binding protein (CREB). Result:Compared with the normal group, the model group showed decreased colon length, increased colon weight, colon weight index, and pathological damage score under colonoscopy, decreased IL-10 level in the colon tissues, increased IL-4, IL-17A, IL-21, and IFN-<italic>γ</italic> levels (<italic>P<</italic>0.05, <italic>P<</italic>0.01), and up-regulated protein expression of TLR2, MyD88, Rac1, IRAK4, IRAK1, TRAF6, TAB1, TAB2, MKK6, p38MAPK, and CREB (<italic>P<</italic>0.01). Compared with the model group, the Sishenwan volatile oil group showed increased colon length, reduced colon weight, colon weight index, and pathological damage score under colonoscopy, elevated IL-10 level in the colon tissues, decreased IL-4, IL-17A, IL-21, and IFN-<italic>γ</italic> levels (<italic>P<</italic>0.05, <italic>P<</italic>0.01),and down-regulated protein expression of TLR2, MyD88, Rac1, IRAK4, IRAK1, TRAF6, TAB1, TAB2, MKK6, p38MAPK, and CREB (<italic>P<</italic>0.05, <italic>P<</italic>0.01). Conclusion:The volatile oil from Sishenwan can effectively improve the inflammatory response of chronic ulcerative colitis, which may be achieved by regulating the TLR/MyD88 signaling pathway.

11.
Article in Chinese | WPRIM | ID: wpr-905849

ABSTRACT

Asari Radix et Rhizoma (ARR) is a traditional Chinese medicine for relieving exterior syndrome, and its roots and stems contain rich chemical components, including volatile oils (terpenoids, aromatics and aliphatics), lignans, flavonoids, etc. Clinically, it has been traditionally used for the treatment of diseases such as phlegm and cough, anemofrigid cold, rheumatic arthralgia due to its ability to spread cold. Modern pharmacological studies have shown that ARR played beneficial roles in analgesic, anti-inflammatory, antitussive, antiasthmatic, antiviral, antibacterial, sedative, antioxidative, and antidepressant responses, antihypertension, as well as tumor suppression. The current studies on the chemical composition of ARR mainly focused on volatile components, and little information is available for the occurrence and pharmacological effects of non-volatile components. In addition, there is a lack of clear classification of chemical components and the distribution of chemical components in medicinal parts and the origin of species. Therefore, in this study, the authors reviewed a large number of literature on the chemical compositions and pharmacological effects of ARR, and hoping to provide a reference for further pharmacological research and the new drug development of ARR.

12.
China Pharmacy ; (12): 2605-2612, 2021.
Article in Chinese | WPRIM | ID: wpr-904518

ABSTRACT

OBJECTIVE:To analysis the correlation between chrom aticity value and quality index of Atractylodis chinensis decoction piece powder stir-fired with bran ,and to determine its processing time. METHODS :The processed samples of 16 batches of A. chinensis decoction piece stir-fired with bran (S0-S15,S0 is the raw product of A. chinensis )were prepared ,and chromaticity values of all samples were determined ,such as lightness value (L*),yellow blue value (b*),red green value (a*). UPLC fingerprint of sample were analyzed ,and the contents of extract and volatile oil were also determined. Pearson correlation was used to analyze the correlation between the chromaticity value and quality index (relative peak area of each chromatographic peak in UPLC fingerprint ,water-soluble extract content ,alcohol-soluble extract content and volatile oil content ). Multivariate statistical analysis (principal component analysis ,cluster analysis ,partial least squares discriminant analysis )was carried out with chromaticity value and quality index ,and the processing time of A. chinensis decoction piece stir-fired with bran was determined by grey correlation method. RESULTS :In the process of bran frying ,with the extension of processing time ,L* and b* of decoction pieces powder decreased ,and a* increased first and then decreased ;relative areas of peak 1 and peak 2 increased first and then decreased,while relative areas of peak 3(5-hydroxymethyl furfural )increased,and the areas of the other peaks decreased. The content of the extract did not change significantly with time ,and the content of the volatile oil decreased. The results of correlation analysis showed that the relative peak area of peak 2-27,alcohol-soluble extract content and volatile oil content had a certain correlation with the chromaticity value ,while the relative peak area of peak 1 and water-soluble extract content had no linear correlation with the chromaticity value. Results of multivariate statistical analysis showed that the samples were divided into mild (S0-S5),excessive (S12-S15),moderate (S6-processing time of 18-33 min). The results of grey correlation method showed that the processing time of A. chinensis decoction piece stir-fired w ith bran should be controlled in the range of 18-24 min,and the optimal processing time was 18 min. CONCLUSIONS :There is a correlation between chromaticity value of A. chinensis decoction piece powder stir-fired with bran and the relative peak area of 27 chromatographic peaks ,and content of extract and volatile oil. It is suggested that the processing time should be 18 min.

13.
China Pharmacy ; (12): 1995-2001, 2021.
Article in Chinese | WPRIM | ID: wpr-886584

ABSTRACT

OBJECTIVE:To establish a metho d for simultaneous determinat ion of α-pinene,β-pinene,myrcene,limonene, γ-terpinene and α-terpineol in volatile oil from Citrus medica . METHODS :GC-QAMS method was adopted. The determination was performed on Agilent DB- 17 capillary column. Flame ionization detector was adopted with nitrogen as carrier gas (purity 99.999%) at the flow rate of 0.5 mL/min. The flow rate of nitrogen (make-up gas )was 25 mL/min,the flow rate of hydrogen was 30 mL/min, and the flow rate of air was 400 mL/min. The inlet temperature was 250 ℃,and the detector temperature was 280 ℃(programmed temperature),and injection volume was 0.5 μL with split ratio of 35 ∶ 1. Using limonene as internal reference ,relative correction factors of α-pinene,β-pinene,myrcene and γ-terpinene were calculated. The contents of 4 components in the samples were calculated according to the relative correction factors. The results were compared between QAMS and internal standard method (ISM,using dodecane as internal substance ,6 components to be determined ). RESULTS :The linear range of α-pinene,β-pinene, myrcene,limonene,γ-terpinene and α-terpineol were 0.030 5-0.213 8,0.066 6-0.466 0,0.021 8-0.152 3,0.765 2-5.356 4,0.387 3- 2.710 8,0.034 2-0.239 6 mg/mL(all r>0.999). The limits of detection were 4.82,7.89,4.01,4.54,5.53,2.47 µg/mL,and the limits of quantification were 15.34,25.91,13.69,15.70,18.68,8.36 µg/mL,respectively. RSDs of precision ,repeatability and stability tests (24 h)were all less than 2%. The average recovery rates were 96.08%,97.48%,100.90%,101.22%,100.54%, 95.84%(all RSD <3%,n=6). The average relative correction factors of α-pinene,β-pinene,myrcene and γ-terpinene were 0.997 8, 1.530 7,0.952 4 and 1.025 5 (all RSD <1%). The contents of α-pinene,β-pinene,myrcene,limonene,γ-terpinene and α-terpineol by ISM were 3.296 9-20.994 1,11.300 6-39.440 9,3.684 2-11.291 4,174.857 8-511.611 8,0-285.127 3,0-48.858 6 mg/g, respectively. The contents of α-pinene,β-pinene,myrcene and γ-terpinene determined by QAMS were 3.296 8-20.994 0,11.300 3- 39.439 7,3.684 1-11.291 1,0-285.126 6 mg/g,respectively. Relative content errors of 4 components as α-pinene determined by 2 methods were all less than 1% . CONCLUSIONS : The Δ 基金项目:四川省科技厅应用基础研究项目(No.2021YJ0114); established GC-QAMS method is simple ,rapid,accurate and 泸州市重点科技项目 [No.2011-S-31(3/3)];合江县人民政府—西南医 科大学战略合作项目(No.2018-HJXNYD-2) repeatable,and can be used for quantitative analysis and *硕士研究生 。研究方向 :中药制备工艺及质量评价 。电话: quality control of volatile oil from C. medica . 0830-3162291。E-mail:493359958@qq.com

14.
China Pharmacy ; (12): 1734-1740, 2021.
Article in Chinese | WPRIM | ID: wpr-882145

ABSTRACT

OBJECTIVE:To optimize the inclusion technology of volatile oil from Ganmao qingre granules. METHODS : Guided by the concept of “quality by design ”,taking volatile oil inclusion rate and inclusion complex yield as key quality attribute,comprehensive score of above two indexes after weighting as response value ,inclusion temperature ,inclusion time ,the ratio of β-CD to volatile oil as key technology parameters ,Box-Burman response surface design was adopted to establish the design space of key technology parameters and key quality attributes. The design space was optimized and verified by 95% confidence interval. The stability of inclusion complex was investigated preliminarily. RESULTS :The optimal design space ,i. e. the optimal technology parameters rang ,included inclusion temperature 35-40 ℃,inclusion time 1.8-2.0 h,the ratio of β-CD to volatile oil 9.5∶1- 10∶1(g/mL). The results of 3 validation tests showed that the volatile oil inclusion rates were all over 62%,the yields of inclusion complex were all over 75%,and the comprehensive scores were all over 80 point. The results of preliminary stability showed that the inclusion rate of volatile oil ,the yield of inclusion complex and the comprehensive score did not change significantly. The difference in evaluation indicators within 7 days was within 5%. CONCLUSIONS :The optimized inclusion technology is feasible , and the obtained inclusion complex is stable.

15.
Article in Chinese | WPRIM | ID: wpr-921799

ABSTRACT

This study was designed to predict the Q-markers of Citri Reticulatae Pericarpium volatile oil and conduct quantitative analysis by GC-MS. The common components of Citri Reticulatae Pericarpium volatile oil were detected by GC-MS. The network pharmacology approaches were utilized for constructing the component-target network and protein-protein interaction(PPI) network, followed by the GO and KEGG pathway enrichment analysis to clarify the pharmacological effects of common components. Molecular docking was conducted to observe the biological activities of common components, thus identifying the Q-markers of Citri Reticulatae Pericarpium volatile oil. The obtained Q-markers were subjected to quantitative analysis by GC-MS. The GC-MS analysis of 19 batches of Citri Reticulatae Pericarpium volatile oil revealed three common components, namely, D-limonene, γ-terpinene, and myrcene. The common components were analyzed based on network pharmacology, and the results showed that Citri Reticulatae Pericarpium volatile oil mainly acted on the core targets GABRA1, GABRA6, GABRA5, GABRA3, and GABRA2 through D-limonene and γ-terpinene, with five important pathways such as nicotine addiction and GABAergic synapse involved. The core targets were mainly distributed in olfactory region, cerebral cortex, cerebellum, basal ganglia, hippocampus, and amygdala to exert the pharmacological effects. As revealed by molecular docking, D-limonene and γ-terpinene exhibited good biological activities, so they were identified as the Q-markers of Citri Reticulatae Pericarpium volatile oil. The results of quantitative analysis showed that the volume fraction of D-limonene was within the range of 0.77-1.03 μL·mL~(-1), and that of γ-terpinene within the range of 0.04-0.13 μL·mL~(-1). The prediction of D-limonene and γ-terpinene as the Q-markers of Citri Reticulatae Pericarpium volatile oil has laid an experimental foundation for the establishment of the quality evaluation standard for Citri Reticulatae Pericarpium volatile oil.


Subject(s)
Citrus , Drugs, Chinese Herbal/pharmacology , Gas Chromatography-Mass Spectrometry , Molecular Docking Simulation , Oils, Volatile/pharmacology
16.
Article in Chinese | WPRIM | ID: wpr-921749

ABSTRACT

Hot melt pressure-sensitive adhesive(HMPSA) has broad application potential in the field of traditional Chinese medicine(TCM) plasters due to its high drug loading, weak skin irritation, satisfactory adhesion, etc. compared with rubber plasters.However, the structure of HMPSA is prone to suffer from the damage caused by volatile oils in TCM plasters. In view of this, a kind of HMPSA with a stable structure was prepared by physical blending of DINCH, polypropylene wax and liquid rubber(LIR) in the present study, which is denoted as DPL. The dosage of cinnamon volatile oil(CVO), the model drug, was selected with viscosity, softening point and cohesion as evaluation indexes. The interaction between DPL and HMPSA was investigated by Fourier transform infrared spectroscopy(FT-IR) and differential scanning calorimetry(DSC). The compatibility of HMPSA with CVO and its transdermal ability were studied by in vitro transdermal test, adhesion, scanning electron microscopy( SEM) and rheological evaluation. The results showed that 5% CVO began to damage the structure of HMPSA. The initial adhesion and holding adhesion of DPL-modified HMPSA(DPL-HMPSA) were not significantly changed compared with those of HMPSA, whereas the 180° peel strength was decreased. FI-IR unraveled that DPL formed the n-π conjugated system with styrene-isoprene-styrene block copolymer(SIS), and there was no significant difference in the glass transition temperature according to DSC results, which indicated the good compatibility of DPL with HMPSA. With 5% CVO loaded, the drug content of DPL-HMPSA was 1. 14 times higher than that of HMPSA, and the decrease rate of drug content in DPL-HMPSA was 16% lower than that in HMPSA after 3 months. SEM demonstrated that CVO did not cause obvious structural damage to DPL-HMPSA. Rheological evaluation revealed that the storage modulus and loss factor of DPL-HMPSA were higher than those of HMPSA, and the cohesion was also stronger. The percutaneous penetration rate of cinnamaldehyde in DPL-HMPSA was 2. 25 times that of HMPSA. In conclusion, DPL-HMPSA had more stable structure, better compatibility with CVO, and higher in vitro transdermal efficiency of cinnamaldehyde than before the modification. This study can provide reference for the mitigation of the matrix structure damage caused by volatile oil components in TCM plasters and the enhancement of the content and in vitro transdermal rate of drug.


Subject(s)
Adhesives , Administration, Cutaneous , Cinnamomum zeylanicum , Oils, Volatile , Spectroscopy, Fourier Transform Infrared
17.
Article in Chinese | WPRIM | ID: wpr-921636

ABSTRACT

Moslae Herba is a commonly used aromatic Chinese medicinal with volatile oil as the main effective component and exhibits broad-spectrum antibacterial and antiviral effects. However, the irritation and instability of Moslae Herba volatile oil necessitate the preparation into a specific dosage form. In this study, the steam distillation method was employed to extract the Moslae Herba volatile oil. The content of thymol and carvacrol in Moslae Herba volatile oil was determined by HPLC as(0.111 9±0.001 0) and(0.235 4±0.004 7) mg·mL~(-1), respectively. Pseudo-ternary phase diagrams and surfactants compounding were applied in the selection of the optimal excipients(surfactant and cosurfactant). On this basis, a nanoemulsion was prepared from the Moslae Herba volatile oil and then loaded into pressure vessels to get sprays, whose stability and antibacterial activity were evaluated afterward. With clarity, viscosity, smell and body feeling as comprehensive indexes, the optimal formulation of the Moslae Herba volatile oil nanoemulsion was determined as follows: Moslae Herba volatile oil∶peppermint oil∶cremophor EL∶absolute ethanol∶distilled water 7.78∶1.58∶19.26∶6.15∶65.23. The as-prepared nanoemulsion was a light yellow transparent liquid, with Tyndall effect shown under the irradiation of parallel light. It has the pH of 5.50, conductivity of 125.9 μS·cm~(-1), average particle size of 15.45 nm, polydispersity index(PDI) of 0.156, and Zeta potential of-17.9 mV. Under a transmission electron microscope, the Moslae Herba volatile oil nanoemulsion was presented as regular spheres without adhesion and agglomeration. Stability test revealed that the Moslae Herba volatile oil nanoemulsion was stable at 4-55 ℃, which was free from demulsification and stratification within 30 days. After the centrifugation at 12 000 r·min~(-1) for 30 min, there was no stratification either. The nanoemulsion had good inhibitory effects on Escherichia coli, Staphylococcus aureus and resistant S. aureus strains, with the minimum inhibitory concentrations of 0.39, 3.12 and 1.56 mg·mL~(-1), respectively. The above results demonstrated that the nanoemulsion was prepared feasibly and showed stable physical and chemical properties and good antibacterial effects. This study provides a practicable technical solution for the development of anti-epidemic and anti-infection products from Moslae Herba volatile oil.


Subject(s)
Anti-Bacterial Agents/pharmacology , Emulsions , Methicillin-Resistant Staphylococcus aureus , Microbial Sensitivity Tests , Oils, Volatile , Particle Size
18.
Article in Chinese | WPRIM | ID: wpr-921629

ABSTRACT

The endophytes of medicinal plants play an important role in promoting the quality formation of the host. Therefore, this paper made a review of endophytes of medicinal plant Atractylodes lancea. According to previous studies, A. lancea boasts endophytes, such as fungi, bacteria, and actinomycetes, among which the beneficial microorganisms help the growth and development of A. lancea. There is a close interaction between the volatile oil of A. lancea and endophytes. Different endophytes vary in regulating the composition and content of the volatile oil of A. lancea, which might contribute to the quality formation of A. lancea. However, the information of the endophytic flora of A. lancea obtained by traditional culture and isolation is not enough to reflect the real situation of the endophytes of A. lancea. Little is known about the endophytes of A. lancea from different chemical types and different habitats, which is not conducive to the study of the ecological relationship between A. lancea and endophytes and limits the development and utilization of the endophytes. Therefore, at the end of this paper, the authors put forward suggestions for future research on endophytes in A. lancea, including:(1)mining the core endophyte resources of A. lancea by combining high-throughput sequencing with traditional culture and isolation;(2)exploring the relationship between the diversity of endophytes and chemical types of A. lancea;(3)strengthening the application of endophytes in A. lancea cultivation, in order to facilitate the cultivation efficiency and quality of A. lancea.


Subject(s)
Atractylodes , Endophytes , Fungi , Oils, Volatile , Plants, Medicinal
19.
Article in Chinese | WPRIM | ID: wpr-879015

ABSTRACT

The volatile oil from Mastiche and Olibanum medicinal materials was extracted by steam distillation, and the chemical components of the volatile oil were analyzed by GC-MS technology. The differences of the volatile oil components were compared and study on the Helicobacter pylori in vitro antimicrobial activitiy was conducted. The results showed that the yields of the volatile oil from Mastiche and Olibanum were 11.93% and 2.40%, respectively. A total of 46 compounds(91.31%) were identified from the volatile oil from Mastiche annd 35 compounds(92.49%) from Olibanum. The classification and comparison study of the components showed that the content of monoterpenes in the volatile oil from Mastiche was the highest(40.69%), followed by alcohols(28.48%); while the content of alcohols in the volatile oil from Olibanum was the highest(35.81%), followed by esters(24.92%). There were significant differences in the components of volatile oil from Mastiche and Olibanum, which might be one of the reasons for the difference in efficacy and application. In vitro bacteriostatic experiments showed that the minimum inhibitory concentration(MIC) of the volatile oil from Mastiche against H. pylori was 1 mg·mL~(-1), and the MIC of the volatile oil from Olibanum against H. pylori was more than 1 mg·mL~(-1). In combination with the results of the oil yield experiment, Mastiche had the advantage of inhibiting H. pylori activity. The research results provide scientific basis for the rational application of Mastiche and Olibanum.


Subject(s)
Anti-Bacterial Agents/pharmacology , Frankincense , Gas Chromatography-Mass Spectrometry , Helicobacter pylori , Monoterpenes/analysis , Oils, Volatile/pharmacology
20.
Article in Chinese | WPRIM | ID: wpr-879000

ABSTRACT

Due to worldwide abuse of chemical antibiotics and continuous emergence of "superbugs", the harm of bacterial drug resistance to human beings has become more and more serious. Therefore, it is of great significance to look for green antibiotics with a wide range of sources, broad antibacterial spectrum, non-toxicity or low toxicity, environmentally friendliness, diverse active components and low drug resistance. The volatile oil of traditional Chinese medicine is a kind of volatile oily liquid that exists in plants and can be distilled with steam and immiscible with water. Because of its good potential to resist drug-resistant pathogens, it is widely used in food, medicine and other fields. This paper summarized the antibacterial advantages and characteristics of volatile oil of traditional Chinese medicine, and the antibacterial effect and antibacterial mechanism of combined application of volatile oil of traditional Chinese medicine, in order to provide some theoretical basis and study ideas for solving the problem of bacterial drug resistance and developing natural and green antibiotics.


Subject(s)
Anti-Bacterial Agents/pharmacology , Anti-Infective Agents , Drugs, Chinese Herbal/pharmacology , Humans , Medicine, Chinese Traditional , Oils, Volatile/pharmacology
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