ABSTRACT
Objective: To research the chemical constituents from Trigonella foenum-graecum. Methods: The chemical constituents were separated and purified by sephadex LH-20, silica gel, semi-prepared HPLC and other chromatography techniques. Their structures were elucidated by their physicochemical properties and NMR data. Results: Six steroidal saponins and flavonoids (1-6) were isolated from the ethanol extracts of T. foenum-graecum and identified as 22-methoxy-trigoneoside IIb (1), gitogenin (2), diosgenin (3), luteolin (4), cynaroside (5), and luteolin-7-O-rutinoside (6). Conclusion: Compound 1 is a new compound, and compounds 5 and 6 are obtained for the first time from T. foenum-graecum.
ABSTRACT
Objective To study chemical constituents from the flowers of Chrysanthemum indicum. Methods The chemical constituents of C. indicum were isolated and purified by various chromatographic techniques, including thin-layer chromatography, silica gel, ODS reversed-phase silica gel, and Sephadex LH-20 for column chromatography, and their structures were identified by NMR spectral analysis. Results Fourteen compounds were isolated and identified as stigmata-4-ene-3-one (1), calenduladiol-3β-O- palmitate (2), 16β,22α-dihydroxypseudotaraxasterol-3β-O-palmitate (3), α-amyrin (4), urs-12-ene-3β,16β-diol (5), 3β-hydroxyurs- 12-ene-11-one (6), arnidiol (7), maniladiol (8), 3β-hydroxyolean-12-ene-11-one (9), luteolin (10), apigenin (11), apigenin-7,4’- dimethyl ether (12), genkwanin (13), and 1-linoleic acid glycerate (14). Conclusion Compounds 1-6, 10-12, and 14 are isolated from the flowers of C. indicum for the first time.
ABSTRACT
Objective: To investigate the chemical constituents from Artemisiae Anomalae Herba (the aerial part of Artemisia anomala or A. actiflora). Methods: The chemical constituents from Artemisiae Anomalae Herba were isolated and purified by chromatography on silica gel and Sephadex LH-20 column, as well as preparative HPLC. Their structures were identified on the base of physicochemical properties and spectroscopic data analyses. Results: Fifteen constituents, including seven steroids and eight flavonoids, were isolated and identified as β-sitosterol (1), β-daucosterol (2), schleicheol 2 (3), α-spinasterol (4), 5α, 8α-epidioxy- ergosta-6, 22-dien-3β-ol (5), 5α, 8α-epidioxy-ergosta-6, 9(11), 22- trien-3β-ol (6), 22E-3β, 5α-dihydroxyergosta-7, 22-dien-6-one (7), naringenin (8), luteolin (9), kaempferol (10), chrysoeriol (11), diosmetin (12), jaceosidin (13), isorhamnetin 3-O-glucoside (14), and hesperetin-7-O-β-D-glucopyranoside (15). Conclusion: Compounds 5, 6, 12-14 are isolated from this plant for the first time, compound 3 is isolated from the plants of Artemisia L. for the first time, and compounds 7 and 15 are isolated from the plants in Compositae family for the first time.