A new steroid from leaves of Dysoxylum binectariferum / 中草药

Objective: To study the constituents of the leaves of Dysoxylum binectariferum. Methods: The chemical constituents were isolated by various column chromatographic methods, and their structures were identified on the basis of MS and NMR spectral analysis. Results: Five steroids were obtained and their structures were elucidated as stigmast-4(5)-en-3β,6β,22-triol (1), stigmast-5(6)-en-3β,21,22-triol (2), 2α,3β,4β-trihydroxypregnan-16-one (3), 2α,3β-dihydroxy-4β-acetoxypregnan-16-one (4) and 2α,3β-dihydroxypregnan-17(20)-en-16-one (5). Conclusion: Compound 1 is identified as a new compound and named dybinstigm A. Compounds 2-5 are obtained from D. binectariferum for the first time.