ABSTRACT
O abortamento na espécie equina é responsável por grandes perdas econômicas e, entre as possíveis causas, está a neosporose, uma enfermidade que nem sempre é investigada como provável diagnóstico. Assim, o objetivo deste trabalho foi relatar um caso de aborto equino aos 129 dias de gestação, resultante da infecção por Neospora spp.. Amostras sanguíneas da égua e do feto abortado foram enviadas para um laboratório especializado. À necrópsia, constatou-se edema gelatinoso e hemorrágico em subcutâneo, fígado ictérico e friável com autólise de alguns órgãos, como baço, rins e glândulas adrenais. Fragmentos dos órgãos coletados na necrópsia foram submetidos à histopatologia e à pesquisa de agentes infecciosos, em que se identificou hepatite e epicardite não purulentas e onfalite purulenta, bem como exame positivo para Neospora spp. pela técnica de reação em cadeia da polimerase (PCR) convencional e Nested. O soro sanguíneo obtido da égua foi submetido à pesquisa de Neospora spp. pela técnica de imunofluorescência indireta, de herpesvírus por soroneutralização em cultura de células e de Leptospira sp. por PCR, todas com resultados negativos. Dessa forma, é importante considerar a neosporose como diagnóstico diferencial em casos de abortamento e natimortalidade, uma vez que a presença de cães nas propriedades é extremamente comum, representando uma importante fonte de infecção.(AU)
Abortion in the equine species is responsible for major economic losses, and among the possible causes is neosporosis, a disease that is not always investigated as a probable diagnosis. Thus, the objective of this study was to report an abortion at 129 days of gestation resulting from Neospora spp. Blood samples from the mare and the aborted fetus were sent to a specialized laboratory. Under necropsy, gelatinous and hemorrhagic edema was detected in subcutaneous tissue, friable and jaundiced liver with autolysis in spleen, kidneys and adrenal glands. Fragments of the organs collected at necropsy were submitted to histopathology and infectious agent tests, which identified non-purulent hepatitis and epicarditis and purulent omphalitis. Also, a positive result for Neospora spp. by the conventional and nested Polymerase Chain Reaction (PCR) technique. Blood serum obtained from the mare was subjected to analyses to Neospora spp. by indirect immunofluorescence technique, herpesvirus by serum neutralization in cell culture and Leptospira sp. by PCR, all with negative results. Thus, it is important to consider neosporosis as a differential diagnosis in cases of abortion and stillbirth, since the presence of dogs in the properties is extremely common and represent an important source of infection.(AU)
Subject(s)
Animals , Female , Neospora/isolation & purification , Abortion, Veterinary/diagnosis , Aborted Fetus/microbiology , Horses/microbiology , Polymerase Chain Reaction/veterinaryABSTRACT
Relata-se o isolamento de Campylobacter jejuni de um feto ovino abortado no terço final da gestação. Em uma semana, observaram-se quatro casos de aborto em ovelhas com quatro meses de gestação. Imediatamente após o terceiro aborto, um dos fetos foi submetido a exame patológico, quando se realizou a colheita do conteúdo do abomaso para análise bacteriológica. A necropsia, somente foram evidenciadas alterações autolíticas. Apenas na microaerofilia encontrou-se crescimento bacteriano puro com características morfo-tintoriais de Campylobacter sp., identificado fenotípica e molecularmente como C. jejuni. Alerta-se para a possibilidade de quadros de abortos em ovinos serem causados por esse patógeno.
Subject(s)
Animals , Abortion, Veterinary/mortality , Campylobacter jejuni/isolation & purification , Aborted Fetus/anatomy & histology , Aborted Fetus/microbiology , Polymerase Chain Reaction , Polymerase Chain Reaction/veterinary , SheepABSTRACT
To study the pathological effect of Brucella organism on the organ's tissues of aborted sheep fetuses, 15 aborted fetuses were used. Brucella organisms were isolated and the fetuses which were positive, their organs were used for pathological study. Then Brucella was isolated from 12 aborted fetuses, examination showed that Br. abortus was the main cause of abortion [percentage 75%], while Br. melitensis was 25%. The histological exam to the organ's of the aborted fetuses which were infected with brucellosis showed that the main organs affected were as follow:- The Brain/ The lesions that were noticed are, diffuse haemorrahges, focal gliosis and neutrophils infiltration within the parenchyma which was in some spots disappeared. The Lungs/ Interstitial pneumonia represented by alveolar blood vessels congestion with inflammatory cells infiltration leading to alveolar wall thickness. The Liver/ Enlargement of the hepatic sinusoids which are filled with blood, the congestion of the central veins with inflammatory cells infiltration especially the neutrophils, also the focuses of small lymphocytic aggregates, and in some cases there was broad degenerative changes in the hepatic tissue. The Kidneys/ Interstitial nephritis represented by the monocytes infiltration especially lymphocytes within the interstitial tissue between the glomerulius and the renal tubules with congestion of the blood vessels and necrotic changes in the renal tubules. The Spleen/ Lymphoid hyperplasia of white pulp and inflammatory cells infiltration within the red pulp. The Testis/ Huge inflammatory cells infiltration especially lymphocyts and neutrophils leading to capsule thicknesses and there is some necrotic and degenerative changes in the seminal tubules. The Heart/ The histological exam of heart muscles showed no significant pathological changes