ABSTRACT
Extensive researches exist in the recent years about attractive properties of nanoparticles and their applicability in design of the biosensors. In this area, development of simple and reliable biosensors for the determination of sulfhydryl compound is pertinent. In this trial, an enzyme-less amperometric biosensor was designed for the detection of cysteine and N-acetyl-L-cysteine. Nanoparticles of iron [III] oxide core-cobalt hexacyanoferrate shell were synthesized and employed as a signal transducer in the amperometric biosensor. The nanoparticles represented a good redox behavior and represented an efficient catalytic activity toward the oxidation of the substrates. Amperometric signals of the biosensor were successfully recorded for quantification of the substrates and the biosensor was successfully employed for the analysis of real samples or determination in blood serum and urine