Mutagenesis of candida albicans by N-Methyl N-Nitro-N-Nitroso guanidine, ultraviolet and acriflavine

N-methyl-N-nitro N-nitroso guanidine [MNNG], Acriflavine [A C] and Ultraviolet [UV] have been used to induce mutation in 4 clinical isolates of C. albicans. Sixteen stable mutants were isolated with MNNG, 11 with AC and 4 mutants with UV. The most lethal effect was recorded, with MNNG [99.48%] and the least lethal action was with UV [77.70%]. UV gives the highest percentage of revertant [73.3%]. The results showed marked differences between the 4 strains to the mutagenic effects of MNNG, AC and UV. These differences could be attributed to differences in the genetic background of the four strains