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1.
Braz. j. microbiol ; 45(1): 205-208, 2014. ilus
Article in English | LILACS | ID: lil-709453

ABSTRACT

The present study reports the first isolation of Actinobacillus seminis from a goat in Brazil. A four-year-old Moxotó breeding goat in a flock of 70 goats and 65 sheep reared together in the county of Patos, semiarid region of Northeastern Brazil, showed clinical signs of unilateral orchitis and epididymitis. Diagnosis of A. seminis infection was confirmed by association of clinical findings, bacterial isolation and 16S rRNA gene sequencing. This result suggests that A. seminis may be an additional cause of infertility in goats, and that sheep may be the source of infection because the mixed farming system allows the contact between sheep and goats in the semiarid region of Northeastern Brazil.


Subject(s)
Animals , Male , Actinobacillus Infections/veterinary , Actinobacillus seminis/isolation & purification , Epididymitis/veterinary , Goat Diseases/microbiology , Orchitis/veterinary , Actinobacillus Infections/complications , Actinobacillus Infections/microbiology , Actinobacillus seminis/classification , Actinobacillus seminis/genetics , Brazil , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Epididymitis/complications , Epididymitis/microbiology , Goats , Orchitis/complications , Orchitis/microbiology , /genetics , Sequence Analysis, DNA
2.
Braz. dent. j ; 18(3): 220-224, 2007. ilus, tab
Article in English | LILACS | ID: lil-471444

ABSTRACT

This study evaluated the transmission of Aggregatibacter actinomycetemcomitans (Aa) in women with severe chronic periodontitis and their children. Thirty women (mean age = 36.1±6.0 years) who were mothers of at least one child aged 7 to 16 years were enrolled. In order to investigate mother-child transmission of Aa, the children were also evaluated when their mothers were colonized by the bacterium. Subgingival plaque samples of each woman were collected from 3 sites (mean probing depth of 7.3±1.2 mm and mean clinical attachment level of 7.9±1.5 mm) and pooled in reduced transport fluid (RTF). These samples were processed, inoculated onto TSBV-agar selective medium and incubated at 37°C in microaerophilic atmosphere for 5 days. Aa was identified on the basis of colony morphology, Gram staining, catalase and oxidase reactions. Aa was found in 8 out of 30 women. Therefore, 8 children from these women (mean age= 12 ± 3.7 years) were evaluated, but Aa was found only in 2 of them. Aa strains of the two mother-child pairs were evaluated by arbitrarily-primed polymerase chain reaction (AP-PCR), although it was not found similarity between the amplitypes of each pair. No Aa transmission was found between Brazilian women with severe chronic periodontitis and their children.


Este estudo avaliou a transmissão de Aggregatibacter actinomycetemcomitans (Aa) entre mulheres com periodontite crônica severa e seus filhos. A amostra constituiu-se de 30 mulheres com idade média de 36,1 ± 6,0 anos, mães de filhos com idade entre 7 e 16 anos. Apenas crianças cujas mães haviam sido colonizadas por Aa foram incluídas. Amostras de placa dentária subgengival foram colhidas de três sítios com profundidade de sonagem média de 7,3 ± 1,2 mm e perda de inserção clínica média de 7,9 ±1,5 mm e agrupadas em fluido de transporte reduzido (RTF). Estas amostras foram processadas e semeadas em meio seletivo ágar TSBV e incubados a 37ºC em atmosfera de microaerofilia por 5 dias. Aa foi identificado baseado na morfologia colonial, coloração de Gram e testes da catalase e oxidase. Aa foi detectado em 8 das 30 mulheres . Assim, 8 filhos destas mulheres, com idade média de 12 ± 3,7 anos foram investigados, mas Aa foi detectado em apenas 2 deles. Cepas de Aa dos 2 pares de mães e filhos foram submetidos a análise pela técnica de reação em cadeia de polimerase usando primers arbitrários (AP-PCR), mas os amplitipos de cada par não demonstraram similaridade. Portanto, não foi encontrada transmissão de Aa entre mulheres brasileiras com periodontite crônica severa e seus filhos.


Subject(s)
Adolescent , Adult , Child , Female , Humans , Male , Aggregatibacter actinomycetemcomitans , Actinobacillus Infections/transmission , Chronic Periodontitis/microbiology , Dental Plaque/microbiology , Infectious Disease Transmission, Vertical , Actinobacillus Infections/complications , Brazil , Colony Count, Microbial , Chronic Periodontitis/complications
3.
Arq. bras. cardiol ; 51(6): 467-469, dez. 1988. tab
Article in Portuguese | LILACS, SES-SP | ID: lil-65542

ABSTRACT

Paciente do sexo masculino de 27 anos de idade, portador de bioprótese valvar cardíaca porcina em posiçäo aórtica há 2,5 anos, sofreu endocardite por Actinobacillus actinomycetemcomitans. A identificaçäo do agente etiológico nas hemoculturas foi possível no sétimo dia de incubaçäo. Houve boa resposta ao tratamento com ampicilina e gentamicina por 42 dias, e o paciente recebeu alta hospitalar


Subject(s)
Humans , Male , Adult , Actinobacillus Infections/complications , Heart Valve Prosthesis/adverse effects , Endocarditis, Bacterial/etiology , Bioprosthesis/adverse effects
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