ABSTRACT
OBJECTIVE@#To investigate the pathological change of mice organ intoxicated by Alangium Chinese and its poisoning mechanism.@*METHODS@#Mice were intoxicated by gavage with extract of Alangium Chinese. Then the histopathologic examination was made for evaluating the pathological changes in the organs of the poisoned mice by HE staining.@*RESULTS@#The main pathological changes included alveolar hemorrhage, pulmonary interstitial hemorrhage, sinus hepaticus expansion and congestion, hepatocyte edema, subarachnoid hemorrhage, congestion and hemorrhage of other organs.@*CONCLUSION@#The main target organs or tissue of Alangium Chinese are the lungs, liver and vascular smooth muscle. There is correlation between the toxic effect and the dosage.
Subject(s)
Animals , Female , Male , Mice , Acute Disease , Alangiaceae/chemistry , Brain/pathology , Disease Models, Animal , Dose-Response Relationship, Drug , Forensic Pathology , Hemorrhage/pathology , Hepatocytes/drug effects , Kidney/pathology , Lethal Dose 50 , Liver/pathology , Lung/pathology , Muscle, Smooth, Vascular/drug effects , Plant Extracts/toxicity , Plant Roots/chemistry , Random Allocation , Toxicity Tests, AcuteABSTRACT
Alangium salviifolium subsp hexapetalum is a medicinal plant which has been traditionally used for tonic and treatment of hemorrhoid. This plant showed promising antimicrobial activity in our preliminary experiments, this study was, therefore, conducted to investigate its inhibitory effect against dermatomycotic organisms and its toxicity. The lyophilized powder extract (4.59%) of pulverized wood was tested for its inhibitory effect by agar disc diffusion test. The extract gave inhibitory zone diameters of 25.23 and 14.78 mm against 26 and 14 isolates of dermatophytes and Candida albicans, respectively. Ketoconazole, used as a reference antifungal agent, had inhibitory zone diameters of 33.15 and 27.93 mm against dermatophytes and C. albicans, respectively. There was no significant difference between the extract and ketoconazole in their inhibition against dermatophytes (p > 0.01), but their difference was significant against C. albicans (p < 0.01). Using Buehler' s method, different amounts of extract (3, 6, and 9 mg/inch2 gauze pad) were tested in five male New Zealand white rabbits. All tested amounts of extract did not induce dermatitis among those rabbits within 1 week. The results demonstrated the inhibitory effect of Alangium salviifolium subsp hexapetalum against fungi without any local toxicity; a tendency to further develop a herbal preparation for the treatment of some dermatomycotic infections.