1.
Biol. Res
; 29(1): 1-11, 1996.
Article
in English
| LILACS
| ID: lil-228541
ABSTRACT
Chemical modification is an important approach to the study of enzyme active sites. This article presents an overview of the methods used. The basic concepts, applications and limitations of chemical modification are outlined. The use of reagents specific for different amino acid side chains is also discussed
Subject(s)
Humans , Amino Acids/chemistry , Enzyme Activation/physiology , Allosteric Site/physiology , Kinetics , Protein Structure, Tertiary
2.
Biol. Res
; 29(1): 47-56, 1996.
Article
in English
| LILACS
| ID: lil-228548
ABSTRACT
Chemical modification is usually employed to study enzyme active sites. Valuable information can also be obtained, however, when this technique is used to probe allosteric sites. This approach is discussed in this article, and it is exemplified in chemical modification studies of the allosteric enzyme phosphofructokinase