ABSTRACT
Mold allergy in frequently involved in the etiopathogenesis of allergy respiratory diseases and it is important for us to determine the fungal classes that most frequently cohabit with men in our region, and under which condition of temperature and humidity they mostly develop. Mycological flora was studied bimonthly in 1989, in 8 homes from Santa Fe City, by exposing three dishes (90 mm) for 20 min. in bathrooms, kitchens and bedrooms. Afterwards, the dishes were incubated for 10 days at 28ºC. Taxonomic identification was made by microscopy of the mature sporulating colonies. Alternaria was the fungal class most frequently found (64 por ciento), followed by Cladospotium (11,4 por ciento), Aspergillus (9,2 por ciento), Curvularia (7 por ciento), Penicillium (4 por ciento), Fusarium (1,2 por ciento) and others (3,2 por ciento). Concerning the rooms, altemaria and Cladosporium were mostly found in kitchens, un like the classes Aspergillus, penicillium and Curvularia that were mostly found in bedrooms. Considering all the colonies as a whole, the totals of each room were: for kitchens 50 por ciento: for bedrooms 28,5 por ciento: and for bathrooms, 21,5 por ciento. The greatest concentration of total colonies was found to those month varied from 70 to 75 por ciento, and the average temperature was stated between 16 and 22ºC. According to the medium values for temperature and humidity in Santa Fe City, spring in the most suitable season for in home molds to develop, being the kitchen the room where a greater number of colonies was found
Subject(s)
Humans , Asthma/etiology , Fungi/pathogenicity , Allergens/immunology , Alternaria/immunology , Alternaria/isolation & purification , Alternaria/pathogenicity , Aspergillus/immunology , Aspergillus/isolation & purification , Aspergillus/pathogenicity , Cladosporium/immunology , Cladosporium/isolation & purification , Cladosporium/pathogenicity , Dust/adverse effects , Fungi/immunology , Fungi/isolation & purification , Humidity/adverse effects , Hypersensitivity/etiology , Hypersensitivity/immunologyABSTRACT
Se estudiaron tres cepas de hongos anemófilos de distintos géneros, que fueron: Alternaria, Rhizopus y Monilia. Encontramos que el medio de cultivo utilizado en esta investigación (Sabouraud líquido) perdía nutrientes a partir de los siete días del crecimiento de las cepas, pero sin afectarse la viabilidad de las mismas. Esto se comprobó por resiembras a los 15 y 60 días. Se elaboraron extractos alergénicos con micelios de esos hongos con siete, 15 y 60 días de crecimiento y se probaron dichos extractos en pacientes supuestamente sensibilizados, por medio de pruebas cutáneas por intradermorreacción, no encontrando diferencias significativas entre los habones obtenidos con estas pruebas. Se recomiendan investigaciones por periodos más prolongados (90,120,180 ó más días) y ver si ello incide en la viabilidad y alergenicidad de los hongos anemófilos.
Subject(s)
Fungi/growth & development , Fungi/immunology , Allergens , Alternaria/growth & development , Alternaria/immunology , Candida/growth & development , Candida/immunology , Cuba , Culture Media/analysis , Rhizopus/growth & development , Rhizopus/immunologyABSTRACT
Foi verificada a presença de esporos de Alternaria durante um período de 12 meses, porém em concentraçöes atmosféricas muito baixas e sem caráter estacional, sendo considerada desprezível